103,190 results match your criteria lectins mammals


3D Structural View of Pathogen Recognition by Mammalian Lectin Receptors.

Front Mol Biosci 2021 25;8:670780. Epub 2021 May 25.

Institute of Molecular Biomembrane and Glycobiology, Division of Structural Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan.

Humans and other mammals resist exogenous pathogens by recognizing them as non-self. How do they do this? The answer lies in the recognition by mammalian lectin receptors of glycans usually found on the surface of pathogens and whose chemical structure is species-specific. Some glycan components, such as galactofuranose, only occur in microbes, and is the principal means by which mammalian lectin receptors recognize non-self. Read More

View Article and Full-Text PDF

A noninvasive diagnostic model for significant liver fibrosis in patients with chronic hepatitis B based on CHI3L1 and routine clinical indicators.

Ann Palliat Med 2021 May;10(5):5509-5519

Clinical Laboratory, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, China.

Background: In consideration of the limitations of liver biopsy, the past years have seen a great advance in the application of noninvasive indices in assessing liver fibrosis. However, the accuracies of the existing indices to determine liver fibrosis in patients with chronic hepatitis B (CHB) are still unsatisfactory. Here, we established a noninvasive diagnostic model for assessing significant liver fibrosis (SLF) in CHB patients based on serum chitinase 3-like 1 (CH3L1) and routine clinical indicators. Read More

View Article and Full-Text PDF

THE ROLE OF GALECTIN-9 IN PATIENTS WITH CHRONIC VIRAL HEPATITIS C AND ITS CONNECTION WITH THE TYPE OF THERAPY, THE DEGREE OF FIBROSIS, CLINICAL, LABORATORY, AUTOIMMUNE AND INTEGRATIVE INDICATORS.

Wiad Lek 2021 ;74(5):1180-1188

SUMY STATE UNIVERSITY, SUMY, UKRAINE.

Objective: The aim: To establish the dependence of the concentration of galectin-9(CGal-9) in the serum of patients with chronic viral hepatitis C (CVHC) on the type of antiviral therapy (AVT), clinical-laboratory, autoimmune and integrative parameters, non-invasive methods of assessing the degree of fibrosis.

Patients And Methods: Materials and methods: CGal-9 in serum were determined in 68 patients with CVHC and 20 healthy individuals, and clinical-laboratory and integrative parameters, noninvasive methods for assessing the degree of fibrosis were studied.

Results: Results:There were three groups: baseline (I), pegylated interferon (PEG-IFN) with ribavirin (II), velpatasvir with sofosbuvir (III). Read More

View Article and Full-Text PDF

A holistic QBD approach to design galactose conjugated PLGA polymer and nanoparticles to catch macrophages during intestinal inflammation.

Mater Sci Eng C Mater Biol Appl 2021 Jul 14;126:112183. Epub 2021 May 14.

Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.

Recruited macrophages in inflammation attract various ligand-receptor drug delivery approaches. Galactose bound nanocarriers are promising to catch macrophages because of surface-expressed macrophage galactose type-lectin-C (MGL-2) receptor. The present study reported fabrication of galactose conjugated PLGA (GAL-PLGA) polymer and nanoparticles under quality by design (QBD) approach to investigate macrophages targeting potential at inflamed intestine. Read More

View Article and Full-Text PDF

Oxidized low-density lipoprotein stimulates dendritic cells maturation via LOX-1-mediated MAPK/NF-κB pathway.

Braz J Med Biol Res 2021 31;54(9):e11062. Epub 2021 May 31.

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.

Dendritic cells (DCs) play a crucial role as central orchestrators of immune system response in atherosclerosis initiation and progression. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is involved in the immune maturation of DCs, but the underlying mechanisms remain unclear. We isolated mouse bone marrow progenitors and stimulated them with granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4 to induce immature DCs. Read More

View Article and Full-Text PDF

Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells.

Int J Mol Sci 2021 May 13;22(10). Epub 2021 May 13.

Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, CZ 142 20 Prague 4, Czech Republic.

Galectin-3 (Gal-3) is a β-galactoside-binding protein that influences various cell functions, including cell adhesion. We focused on the role of Gal-3 as an extracellular ligand mediating cell-matrix adhesion. We used human adipose tissue-derived stem cells and human umbilical vein endothelial cells that are promising for vascular tissue engineering. Read More

View Article and Full-Text PDF

High Levels of the Cleaved Form of Galectin-9 and Osteopontin in the Plasma Are Associated with Inflammatory Markers That Reflect the Severity of COVID-19 Pneumonia.

Int J Mol Sci 2021 May 7;22(9). Epub 2021 May 7.

Department of Respiratory Medicine, Sendai City Hospital, Miyagi 982-8502, Japan.

Numbers of patients with coronavirus disease 2019 (COVID-19) have increased rapidly worldwide. Plasma levels of full-length galectin-9 (FL-Gal9) and osteopontin (FL-OPN) as well as their truncated forms (Tr-Gal9, Ud-OPN, respectively), are representative inflammatory biomarkers. Here, we measured FL-Gal9, FL-OPN, Tr-Gal9, and Ud-OPN in 94 plasma samples obtained from 23 COVID-19-infected patients with mild clinical symptoms (CV), 25 COVID-19 patients associated with pneumonia (CP), and 14 patients with bacterial infection (ID). Read More

View Article and Full-Text PDF

[Knockdown of calreticulin increases intracellular Ca concentration and promotes apoptosis of HSC-LX2 human hepatic stellate cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jun;37(6):501-506

Medical Experimental Research Center, College of Public Health, North China University of Science and Technology, Tangshan 063210, China.*Corresponding author, E-mail:

Objective To investigate the effect of silencing calreticulin (CALR) gene on the apoptosis and intracellular Ca concentration in HSC-LX2 human hepatic stellate cells. Methods Small interfering RNA (siRNA) targeting CALR was designed and transfected into HSC-LX2 cells by lipofectamine transfection, and then the cells with CALR knockdown were screened out. Apoptosis was detected by flow cytometry combined with annexin V-FITC/PI labeling. Read More

View Article and Full-Text PDF

Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.

Front Immunol 2021 14;12:641360. Epub 2021 May 14.

Center for Allergy & Clinical Immunology Research (ACIR), National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Human SP-D is a potent innate immune molecule whose presence at pulmonary mucosal surfaces allows its role in immune surveillance against pathogens. Higher levels of serum SP-D have been reported in the patients with severe acute respiratory syndrome coronavirus (SARS-CoV). Studies have suggested the ability of human SP-D to recognise spike glycoprotein of SARS-CoV; its interaction with HCoV-229E strain leads to viral inhibition in human bronchial epithelial (16HBE) cells. Read More

View Article and Full-Text PDF

Screening of somatic mutations in the JAK2 and CALR genes by high-resolution melting curve analysis.

Klin Lab Diagn 2021 May;66(5):315-320

Central Research Institute of Epidemiology Rospotrebnadzor.

Somatic mutations associated with oncological diseases, including Ph-myeloproliferative neoplasms (Ph-MPN), are very diverse, occur with different frequencies and different allelic burden levels. Therefore, at the initial stage of performing molecular-genetic diagnostic procedures, it is desirable to be able to conduct screening tests in the laboratory. This is especially important when analyzing rare and diverse mutations. Read More

View Article and Full-Text PDF

[Value of chitinase-like protein YKL-40 in bronchoalveolar lavage fluid for predicting refractory pneumonia in children].

Zhongguo Dang Dai Er Ke Za Zhi 2021 May;23(5):471-474

Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou 510010, China.

Objective: To evaluate the value of chitinase-like protein YKL-40 in bronchoalveolar lavage fluid (BALF) for predicting refractory pneumonia (RMPP) in children.

Methods: A total of 50 children with common pneumonia (MPP) and 22 children with RMPP were enrolled. The two groups were compared in terms of clinical features, laboratory examination results, imaging findings, and YKL-40 levels in BALF. Read More

View Article and Full-Text PDF

DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist.

PLoS Pathog 2021 05 20;17(5):e1009576. Epub 2021 May 20.

Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, Grenoble, France.

The efficient spread of SARS-CoV-2 resulted in a unique pandemic in modern history. Despite early identification of ACE2 as the receptor for viral spike protein, much remains to be understood about the molecular events behind viral dissemination. We evaluated the contribution of C-type lectin receptors (CLRS) of antigen-presenting cells, widely present in respiratory mucosa and lung tissue. Read More

View Article and Full-Text PDF

Distinct temporal characteristics of circulating alveolar epithelial and endothelial injury markers in ARDS with COVID-19.

Crit Care 2021 05 17;25(1):169. Epub 2021 May 17.

Department of Anesthesiology and Critical Care Medicine, School of Medicine, Yokohama City University, 3-9, Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan.

View Article and Full-Text PDF

Galectin-3: A factotum in carcinogenesis bestowing an archery for prevention.

Tumour Biol 2021 ;43(1):77-96

Regional Cancer Centre, Thiruvananthapuram, Kerala, India.

Cancer metastasis and therapy resistance are the foremost hurdles in oncology at the moment. This review aims to pinpoint the functional aspects of a unique multifaceted glycosylated molecule in both intracellular and extracellular compartments of a cell namely galectin-3 along with its metastatic potential in different types of cancer. All materials reviewed here were collected through the search engines PubMed, Scopus, and Google scholar. Read More

View Article and Full-Text PDF

Plasma Lectin Pathway Complement Proteins in Patients With COVID-19 and Renal Disease.

Front Immunol 2021 29;12:671052. Epub 2021 Apr 29.

Department of Biomedicine, Aarhus University, Aarhus, Denmark.

We do not understand why non-white ethnicity and chronic kidney disease increase susceptibility to COVID-19. The lectin pathway of complement activation is a key contributor to innate immunity and inflammation. Concentrations of plasma lectin pathway proteins influence pathway activity and vary with ethnicity. Read More

View Article and Full-Text PDF

Water-soluble lectin (WSMoL) from Moringa oleifera seeds treatment recovers glycemic levels and improves left ventricular ejection fraction on Type-2 Diabetes mice model.

An Acad Bras Cienc 2021 10;93(3):e20201596. Epub 2021 May 10.

Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, Brazil.

Moringa oleifera, a plant widely used in traditional medicine as well as for water purification, contains a lectin on its seeds named WSMoL which modulates several immune characteristics and has shown cardiac safe properties. Here, we tested the hypothesis that WSMoL is able to recover fasting glucose levels and to improve the cardiac left ventricular (LV) function in a type 2 diabetes mellitus (T2DM) mice model. T2DM was induced in adult C57BL/6 mice by combining a high fat diet and low doses of Streptozotocin. Read More

View Article and Full-Text PDF

Asymmetric cell division shapes naive and virtual memory T-cell immunity during ageing.

Nat Commun 2021 05 11;12(1):2715. Epub 2021 May 11.

Institute of Microbiology, ETH Zürich, Zurich, Switzerland.

Efficient immune responses rely on heterogeneity, which in CD8 T cells, amongst other mechanisms, is achieved by asymmetric cell division (ACD). Here we find that ageing, known to negatively impact immune responses, impairs ACD in murine CD8 T cells, and that this phenotype can be rescued by transient mTOR inhibition. Increased ACD rates in mitotic cells from aged mice restore the expansion and memory potential of their cellular progenies. Read More

View Article and Full-Text PDF

Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect.

BMC Ophthalmol 2021 May 11;21(1):208. Epub 2021 May 11.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Jinsui Road 7, Tianhe District, 510000, Guangzhou, China.

Background: A reliable and effective method is required to deliver agent that can aid the in vivo imaging of retinal vessels. The aim of the present study was to evaluate retro-orbital (RO) injection of fluorescein-labeled isothiocyanate dextran (FITC-dextran) as a method of demonstrating retinal neovascularization (NV) and avascular areas in oxygen-induced retinopathy (OIR) mice.

Methods: Different concentrations of FITC-dextran were used to compare the efficacy of this agent in perfusing the retinal vessels. Read More

View Article and Full-Text PDF

Emerging role of galectin family in inflammatory autoimmune diseases.

Autoimmun Rev 2021 Jul 7;20(7):102847. Epub 2021 May 7.

Department of Rheumatology and Immunology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China. Electronic address:

Galectin family is a group of glycan-binding proteins. Members in this family are expressed in different tissues, immune or non-immune cells. These molecules are important regulators in innate and adaptive immune response, performing significantly in a broad range of cellular and pathophysiological functions, such as cell proliferation, adhesion, migration, and invasion. Read More

View Article and Full-Text PDF

Involvement of Galectin-1 Tumor Microenvironment in Radiosensitivity.

Anticancer Res 2021 May;41(5):2321-2331

Department of Radiation Oncology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan, R.O.C.;

Background/aim: The mechanisms of galectin-1 in radioresistance may not only involve intracellular but also extracellular effects because galectin-1 can be secreted into the extracellular matrix. We, therefore, aimed to investigate the role of the galectin-1 tumor microenvironment on radiosensitivity in a murine tumor model.

Materials And Methods: Wild-type or stable galectin-1-down-regulated cancer cells (melanoma (B16F10) and lung cancer (LLC1)) were injected (subcutaneous injection) into wild-type or knockout (galectin-1, B cells, and T cells) mice that were subject to 0 or 8 Gy irradiation. Read More

View Article and Full-Text PDF

Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection.

mBio 2021 05 4;12(3). Epub 2021 May 4.

School of Dentistry, Division of Foundational Sciences, University of Alberta, Edmonton, Alberta, Canada

The outbreak of SARS-CoV-2 infection has enormously impacted our lives. Clinical evidence has implicated the emergence of cytokine release syndrome as the prominent cause of mortality in COVID-19 patients. In this study, we observed massive elevation of plasma Galectin-9 (Gal-9) in COVID-19 patients compared to healthy controls (HCs). Read More

View Article and Full-Text PDF

Exploring the Ion Channel TRPV2 and Testicular Macrophages in Mouse Testis.

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

Cell Biology-Anatomy III, Biomedical Center Munich (BMC), Faculty of Medicine, Ludwig-Maximilian-University (LMU), D-82152 Planegg-Martinsried, Germany.

The cation channel TRPV2 is known to be expressed by murine macrophages and is crucially involved in their functionality. Macrophages are frequent cells of the mouse testis, an immune-privileged and steroid-producing organ. TRPV2 expression by testicular macrophages and possible changes associated with age or inflammation have not been investigated yet. Read More

View Article and Full-Text PDF

Detecting the Mechanism behind the Transition from Fixed Two-Dimensional Patterned Sika Deer () Dermal Papilla Cells to Three-Dimensional Pattern.

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

School of Life Sciences, Jilin University, Changchun 130012, China.

The hair follicle dermal papilla is critical for hair generation and de novo regeneration. When cultured in vitro, dermal papilla cells from different species demonstrate two distinguishable growth patterns under the conventional culture condition: a self-aggregative three dimensional spheroidal (3D) cell pattern and a two dimensional (2D) monolayer cell pattern, correlating with different hair inducing properties. Whether the loss of self-aggregative behavior relates to species-specific differences or the improper culture condition remains unclear. Read More

View Article and Full-Text PDF

Insights on the Functional Role of Beta-Glucans in Fungal Immunity Using Receptor-Deficient Mouse Models.

Int J Mol Sci 2021 Apr 30;22(9). Epub 2021 Apr 30.

Laboratory of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Understanding the host anti-fungal immunity induced by beta-glucan has been one of the most challenging conundrums in the field of biomedical research. During the last couple of decades, insights on the role of beta-glucan in fungal disease progression, susceptibility, and resistance have been greatly augmented through the utility of various beta-glucan cognate receptor-deficient mouse models. Analysis of dectin-1 knockout mice has clarified the downstream signaling pathways and adaptive effector responses triggered by beta-glucan in anti-fungal immunity. Read More

View Article and Full-Text PDF

Examination of Surfactant Protein D as a Biomarker for Evaluating Pulmonary Toxicity of Nanomaterials in Rat.

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyusyu 807-8555, Fukuoka, Japan.

This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high toxicity, nanomaterials of two types of titanium dioxides and zinc oxide were classified as having low toxicity, and rat biological samples obtained from 3 days to 6 months after intratracheal instillation of those nanomaterials and micron-particles of crystalline silica were used. There were different tendencies of increase between the high- and low-toxicity materials in the concentration of SP-D in bronchoalveolar-lavage fluid (BALF) and serum and in the expression of the SP-D gene in the lung tissue. Read More

View Article and Full-Text PDF

Myocardial Accumulations of Reg3A, Reg3γ and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Department of Cardiac Surgery, Kerckhoff Heart Center, Benekestr. 2-8, 61231 Bad Nauheim, Germany.

Cardiac sarcoidosis (CS) is a poorly understood disease and is characterized by the focal accumulation of immune cells, thus leading to the formation of granulomata (GL). To identify the developmental principles of fatal GL, fluorescence microscopy and Western blot analysis of CS and control patients is presented here. CS is visualized macroscopically by positron emission tomography (PET)/ computed tomography (CT). Read More

View Article and Full-Text PDF

Melanoma Associated Chitinase 3-Like 1 Promoted Endothelial Cell Activation and Immune Cell Recruitment.

Int J Mol Sci 2021 Apr 10;22(8). Epub 2021 Apr 10.

Department of Dermatology and Venerology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany.

Chitinase 3-like 1 (CHI3L1) is an enzymatically inactive mammalian chitinase that is associated with tumor inflammation. Previous research indicated that CHI3L1 is able to interact with different extracellular matrix components, such as heparan sulfate. In the present work, we investigated whether the interaction of CHI3L1 with the extracellular matrix of melanoma cells can trigger an inflammatory activation of endothelial cells. Read More

View Article and Full-Text PDF

Degraded Arabinogalactans and Their Binding Properties to Cancer-Associated Human Galectins.

Int J Mol Sci 2021 Apr 14;22(8). Epub 2021 Apr 14.

Department of Pharmaceutical Biology, Pharmaceutical Institute, Christian-Albrechts-University of Kiel, 24118 Kiel, Germany.

Galectins represent β-galactoside-binding proteins with numerous functions. Due to their role in tumor progression, human galectins-1, -3 and -7 (Gal-1, -3 and -7) are potential targets for cancer therapy. As plant derived glycans might act as galectin inhibitors, we prepared galactans by partial degradation of plant arabinogalactan-proteins. Read More

View Article and Full-Text PDF

Algae-Derived Bioactive Molecules for the Potential Treatment of SARS-CoV-2.

Molecules 2021 Apr 8;26(8). Epub 2021 Apr 8.

School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.

The recently emerged COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has adversely affected the whole world. As a significant public health threat, it has spread worldwide. Scientists and global health experts are collaborating to find and execute speedy diagnostics, robust and highly effective vaccines, and therapeutic techniques to tackle COVID-19. Read More

View Article and Full-Text PDF