187,537 results match your criteria leads formation


LAMP-H-responsive electrochemical ratiometric biosensor with minimized background signal for highly sensitive assay of specific short-stranded DNA.

Biosens Bioelectron 2021 Sep 22;195:113662. Epub 2021 Sep 22.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:

Herein, the sequence-specific short-stranded biomarker DNA (hDNA, 21-nt) is acted as targeting out-primer to implement the loop-mediated isothermal amplification for releasing hydrogen ions (LAMP-H). Using LAMP-H as signaling transducer, we report a highly sensitive electrochemical ratiometric biosensor for hDNA with minimized background signal, which is achieved via magnetic separation using AuNPs-modified FeO ([email protected]) as micro-reactor. In [email protected], a double-stranded complex of a pH-responsible strand (I*) and a substrate strand (S*) is bound via Au-N bonds, where the treatment with LAMP-H leads to I* folding into i-motif conformation and S* dehybridization. Read More

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September 2021

Selective elimination of CML stem/progenitor cells by picropodophyllin in vitro and in vivo is associated with p53 activation.

Biochem Biophys Res Commun 2021 Sep 17;579:1-7. Epub 2021 Sep 17.

School of Food Sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China; Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, People's Republic of China. Electronic address:

Chronic myeloid leukemia (CML) is a hematologic malignancy originating from BCR-ABL oncogene-transformed hematopoietic stem cells (HSCs) known as leukemia stem cells (LSCs). Therefore, targeting LSCs is of primary importance to eradicate CML. The present study demonstrates that picropodophyllin (PPP) effectively induces apoptosis and inhibits colony formation in CML stem/progenitor cells as well as quiescent CML progenitors resistant to imatinib therapy, while sparing normal hematopoietic cells in vitro. Read More

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September 2021

Metreleptin treatment of non-HIV lipodystrophy syndromes.

Presse Med 2021 Sep 24:104070. Epub 2021 Sep 24.

Department of Endocrinology, Diabetology and Metabolism, Lille University Hospital, F-59000 Lille, France; University of Lille, F-59000 Lille, France; INSERM U1190, European Genomic Institute for Diabetes, F-59000 Lille, France.. Electronic address:

Lipodystrophy syndromes (LS) constitute a group of rare diseases of the adipose tissue, characterized by a complete or selective deficiency of the fat mass. These disorders are associated with important insulin resistance, cardiovascular and metabolic comorbidities that impact patient's survival and quality of life. Management is challenging and includes diet, physical activity, and specific pharmacological treatment of LS-associated comorbidities. Read More

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September 2021

Cytokine exposure mediates transcriptional activation of the orphan nuclear receptor Nur77 in hematopoietic cells.

J Biol Chem 2021 Sep 24:101240. Epub 2021 Sep 24.

Department of Internal Medicine, Washington University, St Louis, Missouri, 63110, USA. Electronic address:

The orphan nuclear receptor Nur77 is an immediate-early response gene that based on tissue and cell context is implicated in a plethora of cellular processes, including proliferation, differentiation, apoptosis, metabolism, and inflammation. Nur77 has a ligand-binding pocket that is obstructed by hydrophobic side-groups. Naturally-occurring, cell-endogenous ligands have not been identified and Nur77 transcriptional activity is thought to be regulated through post-translational modification and modulation of protein levels. Read More

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September 2021

Human embryo polarization requires PLC signaling to mediate trophectoderm specification.

Elife 2021 Sep 27;10. Epub 2021 Sep 27.

Department of Physiology, Neuroscience and Development, University of Cambridge, Cambridgeshire, United Kingdom.

Apico-basal polarization of cells within the embryo is critical for the segregation of distinct lineages during mammalian development. Polarized cells become the trophectoderm (TE), which forms the placenta, and apolar cells become the inner cell mass (ICM), the founding population of the fetus. The cellular and molecular mechanisms leading to polarization of the human embryo and its timing during embryogenesis have remained unknown. Read More

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September 2021

Droplet Formation and Growth Mechanisms in Reaction-Induced Spontaneous Emulsification of 3-(Trimethoxysilyl) Propyl Methacrylate.

Langmuir 2021 Sep 27. Epub 2021 Sep 27.

Chemical and Biomolecular Engineering Department, Johns Hopkins University, Baltimore, Maryland 21218, United States.

Spontaneous emulsification of 3-(trimethoxysilyl) propyl methacrylate (TPM) can produce complex and active colloids, nanoparticles, or monodisperse Pickering emulsions. Despite the applicability of TPM in particle synthesis, the nucleation and growth mechanisms of TPM emulsions are still poorly understood. We investigate droplet formation and growth of TPM in aqueous solutions under quiescent conditions. Read More

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September 2021

[Aciduria argininosuccínica: informe de un caso de inicio neonatal].

Arch Argent Pediatr 2021 Oct;119(5):e508-e512

Servicio de Crecimiento y Desarrollo, Hospital Pediátrico Dr. H. J. Notti, Mendoza, Argentina.

Urea cycle defects are inborn errors of metabolism produced by a defect in one of the enzymes responsible for the detoxification of ammonia, which generates its accumulation in the body. The clinical manifestations can present early, with high morbidity and mortality, or late onset. The heterogeneity of the symptoms and the lack of clinical suspicion in neonates leads to a wrong diagnosis, which can be confused with neonatal sepsis or cerebral hemorrhages. Read More

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October 2021

A quantitative multiscale perspective on primary olefin formation from methanol.

Phys Chem Chem Phys 2021 Sep 27. Epub 2021 Sep 27.

Cardiff Catalysis Institute, School of Chemistry, Cardiff University, Park Place, Cardiff CF10 3AT, UK.

The formation of the first C-C bond and primary olefins from methanol over zeolite and zeotype catalysts has been studied for over 40 years. Over 20 mechanisms have been proposed for the formation of the first C-C bond. In this quantitative multiscale perspective, we decouple the adsorption, desorption, mobility, and surface reactions of early species through a combination of vacuum and sub-vacuum studies using temporal analysis of products (TAP) reactor systems, and through studies with atmospheric fixed bed reactors. Read More

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September 2021

Dyslipidemia in Chronic Kidney Disease: Contemporary Concepts and Future Therapeutic Perspectives.

Am J Nephrol 2021 Sep 17:1-9. Epub 2021 Sep 17.

Department of Nephrology, General Hospital of Nikaia-Piraeus, Athens, Greece.

Background: Chronic kidney disease (CKD) is an increasingly prevalent disease state met with great morbidity and mortality primarily resulting from the high incidence of adverse cardiovascular outcomes. Therapeutic strategies in this patient population aim at controlling modifiable cardiovascular risk factors, including dyslipidemia.

Summary: In this review article, we first provide the latest pathophysiologic evidence regarding the altered dyslipidemia pattern in CKD, followed by its contemporary management according to the latest guidelines. Read More

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September 2021

Strong Acids or Bases Displaced by Weak Acids or Bases in Aerosols: Reactions Driven by the Continuous Partitioning of Volatile Products into the Gas Phase.

Acc Chem Res 2021 Sep 27. Epub 2021 Sep 27.

Key Laboratory of Cluster Science, Ministry of Education of China, The Institute of Chemical Physics, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P.R. China.

ConspectusAerosols are ubiquitous in the atmosphere and profoundly affect climate systems and human health. To gain more insights on their broad impacts, we need to comprehensively understand the fundamental properties of atmospheric aerosols. Since aerosols are multiphase, a dispersion of condensed matter (solid particles or liquid droplets, hereafter particles) in gas, partitioning of volatile matter between the condensed and the gas phases is one defining characteristic of aerosols. Read More

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September 2021

Intercalation of a Cationic Cyanine Dye Assisted by Anionic Surfactants within Mg-Al Layered Double Hydroxide.

ACS Omega 2021 Sep 10;6(37):23837-23845. Epub 2021 Sep 10.

Global Center for Science and Engineering, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8050, Japan.

An original route for the intercalation of a 1,1'-diethyl-2,2'-cyanine iodide (PIC) cationic dye, through the use of anionic surfactants as vector/carrier phases, within Mg-Al layered double hydroxide (LDH) was investigated. From the data acquired from complementary techniques (X-ray diffraction, infrared and UV-visible spectroscopies, thermogravimetry, and fluorimetry), it appears that both the intercalation and aggregation states of the cationic dye within the internal structure of LDH mainly depend on both the surfactant state (monomer form or spherical micelle) and its amount. The intercalation of PIC at a low molar ratio to the anionic surfactant leads to the formation of -aggregates with singular fluorescence properties that mainly depend on the nature of the anionic surfactant used for the co-intercalation process. Read More

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September 2021

Regenerative Medicine for the Treatment of Ischemic Heart Disease; Status and Future Perspectives.

Front Cell Dev Biol 2021 10;9:704903. Epub 2021 Sep 10.

Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Cardiovascular disease is now the leading cause of adult death in the world. According to new estimates from the World Health Organization, myocardial infarction (MI) is responsible for four out of every five deaths due to cardiovascular disease. Conventional treatments of MI are taking aspirin and nitroglycerin as intermediate treatments and injecting antithrombotic agents within the first 3 h after MI. Read More

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September 2021

Disruption of Photomorphogenesis Leads to Abnormal Chloroplast Development and Leaf Variegation in .

Front Plant Sci 2021 9;12:720800. Epub 2021 Sep 9.

Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China.

cv. 'Yanlingyinbiancha' is a leaf-variegated mutant with stable genetic traits. The current study aimed to reveal the differences between its albino and green tissues, and the molecular mechanism underlying the variegation. Read More

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September 2021

Insights Into the Molecular Mechanisms of Polycystic Kidney Diseases.

Front Physiol 2021 8;12:693130. Epub 2021 Sep 8.

Department of Physiology, Augusta University, Augusta, GA, United States.

Autosomal dominant (AD) and autosomal recessive (AR) polycystic kidney diseases (PKD) are severe multisystem genetic disorders characterized with formation and uncontrolled growth of fluid-filled cysts in the kidney, the spread of which eventually leads to the loss of renal function. Currently, there are no treatments for ARPKD, and tolvaptan is the only FDA-approved drug that alleviates the symptoms of ADPKD. However, tolvaptan has only a modest effect on disease progression, and its long-term use is associated with many side effects. Read More

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September 2021

Nerve Growth Factor-Based Therapy in Alzheimer's Disease and Age-Related Macular Degeneration.

Front Neurosci 2021 9;15:735928. Epub 2021 Sep 9.

Research Laboratories in Ophthalmology, IRCCS-Fondazione Bietti, Rome, Italy.

Alzheimer's disease (AD) is an age-associated neurodegenerative disease which is the most common cause of dementia among the elderly. Imbalance in nerve growth factor (NGF) signaling, metabolism, and/or defect in NGF transport to the basal forebrain cholinergic neurons occurs in patients affected with AD. According to the cholinergic hypothesis, an early and progressive synaptic and neuronal loss in a vulnerable population of basal forebrain involved in memory and learning processes leads to degeneration of cortical and hippocampal projections followed by cognitive impairment with accumulation of misfolded/aggregated Aβ and tau protein. Read More

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September 2021

Protein Immobilization on Heterogeneous (SiO/ZnO) Hollow-Mesoporous Nanocapsules Prepared by Imprinting CPMV: Drug Delivery and Possibility of Immunological Applications.

ACS Biomater Sci Eng 2021 Sep 25. Epub 2021 Sep 25.

School of Biomedical Engineering, Indian Institute of Technology (BHU), Varanasi 220 051, India.

Herein, we report the protein immobilization and stability studies of heterogeneous hollow mesoporous nanocapsules () for drug and protein delivery. The final results of the diverse precursors such as TEOS, TMOS, APTES, and zinc acetate on the formation of the hollow-mesoporous architecture of nanocapsules have been assimilated in this work. Three types of of various sizes were synthesized. Read More

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September 2021

Wetting-Induced Polyelectrolyte Pore Bridging.

Membranes (Basel) 2021 Aug 31;11(9). Epub 2021 Aug 31.

DWI-Leibniz-Institute for Interactive Materials, Forckenbeckstrasse 50, 52074 Aachen, Germany.

Active layers of ion separation membranes often consist of charged layers that retain ions based on electrostatic repulsion. Conventional fabrication of these layers, such as polyelectrolyte deposition, can in some cases lead to excess coating to prevent defects in the active layer. This excess deposition increases the overall membrane transport resistance. Read More

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KLF15 Regulates Oxidative Stress Response in Cardiomyocytes through NAD.

Metabolites 2021 Sep 13;11(9). Epub 2021 Sep 13.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77004, USA.

KLF15 has recently emerged as a central regulator of metabolism. Although its connection to oxidative stress has been suspected, there has not been any study to date that directly demonstrates the molecular link. In this study, we sought to determine the role of KLF15 in cardiac oxidative stress. Read More

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September 2021

Dual role of a dedicated GAPDH in the biosynthesis of volatile and non-volatile metabolites- novel insights into the regulation of secondary metabolism in Trichoderma virens.

Microbiol Res 2021 Sep 9;253:126862. Epub 2021 Sep 9.

Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India. Electronic address:

Trichoderma virens produces viridin/viridiol, heptelidic (koningic) acid, several volatile sesquiterpenes and gliotoxin (Q strains) or gliovirin (P strains). We earlier reported that deletion of the terpene cyclase vir4 and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH, designated as vGPD) associated with the "vir" cluster abrogated the biosynthesis of several volatile sesquiterpene metabolites. Here we show that, the deletion of this GAPDH also impairs the biosynthesis of heptelidic acid (a non-volatile sesquiterpene), viridin (steroid) and gliovirin (non-ribosomal peptide), indicating regulation of non-volatile metabolite biosynthesis by this GAPDH that is associated with a secondary metabolism gene cluster. Read More

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September 2021

Protective Effects of Melatonin against Obesity-Induced by Leptin Resistance.

Behav Brain Res 2021 Sep 23:113598. Epub 2021 Sep 23.

Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India. Electronic address:

Consumption of an exceedingly high-fat diet with irregular eating and sleeping habits is typical in the current sedentary lifestyle, leading to chronic diseases like obesity and diabetes mellitus. Leptin is a primary appetite-regulating hormone that binds to its receptors in the hypothalamic cell membrane and regulates downstream appetite-regulating neurons NPY/AgRp and POMC in the hypothalamus. Based on the fat content of the adipose tissue, leptin is secreted, and excess accumulation of fat in adipose tissue stimulates the abnormal secretion of leptin. Read More

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September 2021

Selective and marked blockade of endothelial sprouting behavior using paclitaxel and related pharmacologic agents.

Am J Pathol 2021 Sep 23. Epub 2021 Sep 23.

Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida School of Medicine, Tampa, FL 33612. Electronic address:

Here, we investigate whether alterations in the microtubule cytoskeleton affect the ability of ECs to sprout and form branching networks of tubes. Using defined bioassays of human EC tubulogenesis, where both sprouting behavior and lumen formation can be rigorously evaluated, we demonstrate that addition of the microtubule stabilizing drugs, paclitaxel, docetaxel, ixabepilone, and epothilone B completely interfere with EC tip cells and sprouting behavior, while allowing for EC lumen formation. In bioassays mimicking vasculogenesis using single or aggregated ECs, these drugs induce ring-like lumens from single cells or cyst-like spherical lumens from multicellular aggregates with no evidence of EC sprouting behavior. Read More

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September 2021

Kynurenine derivative 3-HAA is an agonist ligand for transcription factor YY1.

J Hematol Oncol 2021 Sep 25;14(1):153. Epub 2021 Sep 25.

Basic Medical Institute, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The 3-hydroxyanthranilic acid (3-HAA), a derivative of kynurenine, was reported to suppress tumor growth. However, the function of 3-HAA largely remains unclear. Here, we report that 3-hydroxyanthranilic acid (3-HAA) is lower in tumor cells, while adding exogenous 3-HAA induces apoptosis in hepatocellular carcinoma by binding YY1. Read More

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September 2021

E484K mutation in SARS-CoV-2 RBD enhances binding affinity with hACE2 but reduces interactions with neutralizing antibodies and nanobodies: Binding free energy calculation studies.

J Mol Graph Model 2021 Sep 17;109:108035. Epub 2021 Sep 17.

The Sixth Laboratory, National Vaccine and Serum Institute, Beijing, 101111, China. Electronic address:

The pandemic of the COVID-19 disease caused by SARS-CoV-2 has led to more than 200 million infections and over 4 million deaths worldwide. The progress in the developments of effective vaccines and neutralizing antibody therapeutics brings hopes to eliminate the threat of COVID-19. However, SARS-CoV-2 continues to mutate, and several new variants have been emerged. Read More

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September 2021

Deep blue autofluorescence reveals the instability of human transthyretin.

Int J Biol Macromol 2021 Sep 22. Epub 2021 Sep 22.

Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.

Wild-type human transthyretin (TTR) is a tetrameric protein that transports thyroxine and retinol in the blood and brain. However, a number of mutations or aging leads to destabilization of the quaternary structure of TTR, which results in dissociation of TTR tetramers to monomers, followed by oligomerization and subsequent amyloid formation. TTR amyloid is a pathogenic factor underlying several diseases. Read More

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September 2021

Pediatric Drug Development Studies for Familial Hypercholesterolemia Submitted to the US Food and Drug Administration Between 2007-2020.

J Clin Pharmacol 2021 Sep 25. Epub 2021 Sep 25.

Office of Pediatric Therapeutics, Commissioner's Office, US Food and Drug Administration, Silver Spring, MD, USA.

Familial hypercholesterolemia (FH) is an autosomal dominant genetic disorder of lipoprotein metabolism that leads to an increased risk of developing atherosclerosis and coronary artery disease. Hypercholesterolemia in pediatric patients is typically due to FH. Treatment of pediatric FH is achieved through lifestyle modifications, lipid-modifying pharmacotherapy, and/or apheresis. Read More

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September 2021

Nanosystems and exosomes as future approaches in treating Multiple sclerosis.

Eur J Neurosci 2021 Sep 24. Epub 2021 Sep 24.

Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland.

Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system which leads to neurological dysfunctions and severe disabilities. MS pathology is characterized by damage of the blood-brain barrier and infiltration of autoreactive T cells that over-activate glial cells, thereby initiating neuroinflammation accompanied by the formation of demyelinating plaques and neurodegeneration. Clinical deficits in this multifactorial disease depend on the progression of myelin loss, the stage of inflammation, the status of axons and the activity of oligodendrocyte precursor cells (OPCs). Read More

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September 2021

Reduced menin expression leads to decreased ERα expression and is correlated with the occurrence of human luminal B-like and ER-negative breast cancer subtypes.

Breast Cancer Res Treat 2021 Sep 24. Epub 2021 Sep 24.

Univ Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France.

Purpose: Menin, encoded by the MEN1 gene, was recently reported to be involved in breast cancers, though the underlying mechanisms remain elusive. In the current study, we sought to further determine its role in mammary cells.

Methods: Menin expression in mammary lesions from mammary-specific Men1 mutant mice was detected using immunofluorescence staining. Read More

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September 2021

X-Linked Hypophosphatemic Rickets: Report of a Novel PHEX Mutation and Cinacalcet as Adjuvant Therapy in the Mineral Metabolism Control.

Mod Rheumatol Case Rep 2021 Sep 25. Epub 2021 Sep 25.

Department of Endocrinology, Instituto Português de Oncologia de Lisboa Francisco Gentil, Lisbon, Portugal.

X-linked hypophosphatemic rickets (XLH) is a rare disease caused by a mutation in the PHEX gene, located on the X chromosome. This gene encodes the phosphate regulating endopeptidase, and its inactivation leads to increased levels of circulating phosphatonins responsible for renal phosphate loss. The treatment of XLH is still carried out with long-term administration of phosphate and calcitriol, which can be complicated by hyperparathyroidism, nephrocalcinosis, renal failure and hypertension. Read More

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September 2021

Disulfiram-loaded lactoferrin nanoparticles for treating inflammatory diseases.

Acta Pharmacol Sin 2021 Sep 24. Epub 2021 Sep 24.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Sepsis is a dysregulated immune response to infection and potentially leads to life-threatening organ dysfunction, which is often seen in serious Covid-19 patients. Disulfiram (DSF), an old drug that has been used to treat alcohol addiction for decades, has recently been identified as a potent inhibitor of the gasdermin D (GSDMD)-induced pore formation that causes pyroptosis and inflammatory cytokine release. Therefore, DSF represents a promising therapeutic for the treatment of inflammatory disorders. Read More

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September 2021

Giant Left Atrial Thrombus Despite Anticoagulation with Apixaban in a Patient with Mitral Stenosis and Atrial Fibrillation.

Am J Case Rep 2021 Sep 25;22:e933162. Epub 2021 Sep 25.

Collegium Medicum, Jan Kochanowski University, Kielce, Poland.

BACKGROUND In patients with atrial fibrillation (AF), the presence of a left atrial thrombus correlates with the highest risk of stroke. Mitral stenosis (MS) is an acquired disease that leads to atrial pressure overload and subsequent significant anatomical and electrical remodeling of the left atrium. This promotes the occurrence of AF and atrial thrombus formation. Read More

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September 2021