856 results match your criteria layered repair

Bio-engineering a prevascularized human tri-layered skin substitute containing a hypodermis.

Acta Biomater 2021 Jul 21. Epub 2021 Jul 21.

Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, University of Zurich, Zurich, Switzerland; Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland. Electronic address:

Severe injuries to skin including hypodermis require full-thickness skin replacement. Here, we bio-engineered a tri-layered human skin substitute (TLSS) containing the epidermis, dermis, and hypodermis. The hypodermal layer was generated by differentiation of human adipose stem cells (ASC) in a collagen type I hydrogel and combined with a prevascularized dermis consisting of human dermal microvascular endothelial cells and fibroblasts, which arranged into a dense vascular network. Read More

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Unilateral Silver-Loaded Silk Fibroin Difunctional Membranes as Antibacterial Wound Dressings.

ACS Omega 2021 Jul 30;6(27):17555-17565. Epub 2021 Jun 30.

Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, China.

Silk fibroin (SF) has been widely used as wound dressings due to its good biocompatibility. To enhance the antibacterial properties of the dressings, silver (Ag) is often added. However, an overdose of Ag may cause cytotoxicity and inhibit wound healing. Read More

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Screw Fixation of Pediatric Tibial Tubercle Fractures.

JBJS Essent Surg Tech 2021 Apr-Jun;11(2). Epub 2021 Jun 10.

Rady Children's Hospital, University of California San Diego, San Diego, California.

Background: Tibial tubercle fractures are rare injuries that account for <1% of physeal fractures. These fractures are thought to be increasing in frequency, particularly in young, adolescent males who participate in basketball and other sports with repeated running and jumping. The tibial apophysis becomes mechanically vulnerable as the proximal tibial physis closes from posteromedial to anterolateral, enabling the quadriceps to overpower the chondroepiphysis and avulse the proximal tibial epiphysis from the tibial metaphysis. Read More

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Wound Management following Total Knee Arthroplasty: An Updated Review.

J Knee Surg 2021 Jul 14. Epub 2021 Jul 14.

Northwell Health Orthopaedics, Lenox Hill Hospital, New York City, New York.

Optimal wound closure techniques following total knee arthroplasty (TKA) have focused on enhancing healing potential, preventing infection, yielding satisfactory cosmesis, and allowing early ambulation and functionality. An appropriate layered closure and management of the TKA typically involves addressing the (1) deep fascial layer; (2) subdermal layer; (3) intradermal layer, including the subcuticular region; and (4) final application of a specific aseptic dressing, each of which are covered here in detail. This focused critical review of the literature discusses traditional techniques used in all layers of wound closure following TKA while introducing several emerging popular techniques. Read More

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Abdominal hernia repair using the Rives-Stoppa technique: an abdominal reconstruction.

Cir Pediatr 2021 Jul 1;34(3):164-167. Epub 2021 Jul 1.

Pediatric Intensive Care Unit, Navarra Hospital Complex. Pamplona (Spain).

Introduction: Retromuscular mesh hernia repair using Rives-Stoppa technique has demonstrated the best results when it comes to repairing large midline hernias. We present the first pediatric case successfully treated with this technique.

Clinical Case: This is the case of a 9-year-old male patient with hernia following urgent midline laparotomy. Read More

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Adhesive and tough hydrogels: from structural design to applications.

J Mater Chem B 2021 Jul 13. Epub 2021 Jul 13.

Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.

In recent years, multifunctional hydrogels have garnered great interest. Usually, there is a contradiction between the toughness and interface adhesion of traditional hydrogels. In engineering and medical applications, hydrogels need to have good adhesive properties and toughness. Read More

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Extradural abscess following synthetic fabric duraplasty.

Surg Neurol Int 2021 25;12:234. Epub 2021 May 25.

School of Medicine, Gandaki Medical College, Pokhara, Gandaki, Nepal.

Background: Duraplasty refers to the neurosurgical process of reconstructing dural defect. Variety of materials is used for such reconstruction, including natural, semisynthetic, and synthetic materials. Although synthetic materials are readily available and easy to apply, these are associated with foreign body reaction which may lead to serious consequences in some cases. Read More

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A Novel Combined Transorbital Transnasal Endoscopic Approach for Reconstruction of Posttraumatic Complex Anterior Cranial Fossa Defect.

Asian J Neurosurg 2021 Jan-Mar;16(1):136-140. Epub 2021 Mar 20.

Department of Neurosurgery, Madras Medical College, Chennai, Tamil Nadu, India.

The transorbital neuroendoscopic surgery (TONES), endoscopic transnasal skull base procedures, and the concept of multiportal minimal access neurosurgery are novel, rapidly evolving approaches in the management of complex skull base lesions. A 27-year-old male presented with a history of road traffic accident with nasal bleed, cerebrospinal fluid (CSF) rhinorrhea, and left eye deformity. There was left upper eyelid ecchymosis, orbital dystopia, left pupil was dilated, and fixed with no extraocular movements. Read More

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Mesenchymal stem cells loaded on 3D-printed gradient poly(ε-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering.

Regen Biomater 2021 Jun 16;8(3):rbab019. Epub 2021 May 16.

Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair, Department of Orthopedics, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.

Cartilage has limited self-repair ability due to its avascular, alymphatic and aneural features. The combination of three-dimensional (3D) printing and tissue engineering provides an up-and-coming approach to address this issue. Here, we designed and fabricated a tri-layered (superficial layer (SL), middle layer (ML) and deep layer (DL)) stratified scaffold, inspired by the architecture of collagen fibers in native cartilage tissue. Read More

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Skin Substitutes for Adults With Diabetic Foot Ulcers and Venous Leg Ulcers: A Health Technology Assessment.


Ont Health Technol Assess Ser 2021 4;21(7):1-165. Epub 2021 Jun 4.

Background: Wounds may be caused in a variety of ways. Some wounds are difficult to heal, such as diabetic foot ulcers and venous leg ulcers. We conducted a health technology assessment of skin substitutes for adults with neuropathic diabetic foot ulcers and venous leg ulcers, which included an evaluation of effectiveness, safety, cost-effectiveness, the budget impact of publicly funding skin substitutes, and patient preferences and values. Read More

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3D-bioprinted gradient-structured scaffold generates anisotropic cartilage with vascularization by pore-size-dependent activation of HIF1α/FAK signaling axis.

Nanomedicine 2021 Jun 24;37:102426. Epub 2021 Jun 24.

Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.

Articular cartilage injury is one of the most common diseases in orthopedics, which seriously affects patients' life quality, the development of a biomimetic scaffold that mimics the multi-layered gradient structure of native cartilage is a new cartilage repair strategy. It has been shown that scaffold topography affects cell attachment, proliferation, and differentiation; the underlying molecular mechanism of cell-scaffold interaction is still unclear. In the present study, we construct an anisotropic gradient-structured cartilage scaffold by three-dimensional (3D) bioprinting, in which bone marrow stromal cell (BMSC)-laden anisotropic hydrogels micropatterns were used for heterogeneous chondrogenic differentiation and physically gradient synthetic poly (ε-caprolactone) (PCL) to impart mechanical strength. Read More

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The Lesser Tuberosity Osteotomy Exposure for Total Shoulder Arthroplasty.

JBJS Essent Surg Tech 2021 Jan-Mar;11(1). Epub 2021 Feb 11.

Department of Orthopedic Surgery, Columbia University Irving Medical Center, New York, NY.

Background: The lesser tuberosity osteotomy (LTO) is a commonly employed technique for mobilizing the subscapularis tendon during anatomic total shoulder arthroplasty that is performed through a deltopectoral approach. During this procedure, the lesser tuberosity is osteotomized from the proximal aspect of the humerus while maintaining the strong tendon-to-bone attachment of the subscapularis tendon insertion. After the shoulder arthroplasty is performed, the lesser tuberosity osseous fragment is then resecured to the proximal aspect of the humerus with heavy nonabsorbable suture, which allows for direct bone-to-bone compression and healing of the fragment to the proximal aspect of the humerus. Read More

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February 2021

A biomimetic triple-layered biocomposite with effective multifunction for dura repair.

Acta Biomater 2021 08 10;130:248-267. Epub 2021 Jun 10.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China. Electronic address:

Dura mater defect and subsequent cerebrospinal fluid (CSF) leakage usually appear in trauma or neurosurgical procedures and are followed by a series of serious complications and even death. The use of a qualified dura mater substitute with multifunction of leakage blockade, adhesion prevention, and dura reconstruction is one of the promising treatment methods. However, even though some products have been used in the clinic, none of the substitutes achieved the required multifunction. Read More

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Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects.

Int J Nanomedicine 2021 3;16:3803-3818. Epub 2021 Jun 3.

Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.

Background: Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall.

Materials And Methods: In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). Read More

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A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings.

Wound Repair Regen 2021 Jun 9. Epub 2021 Jun 9.

Center for Microbial Communities, Aalborg University, Aalborg East, Denmark.

Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. Read More

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Systematic analysis of molecular characterization and clinical relevance of m6A regulators in digestive system pan-cancers.

Exp Biol Med (Maywood) 2021 Jun 8:15353702211019681. Epub 2021 Jun 8.

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing 100032, China.

Digestive system tumors, which mainly include esophagus, stomach, colorectum, liver, pancreas, bile duct, and some other tumors, often have a poor prognosis. N6-methyladenosine (m6A) has critical functions in development and tumorigenesis and may help improve the molecular mechanisms of digestive system tumors. However, current understanding of the reconstitution of m6A in digestive system tumors is far from comprehensive. Read More

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Preclinical assessment on neuronal regeneration in the injury-related microenvironment of graphene-based scaffolds.

NPJ Regen Med 2021 Jun 2;6(1):31. Epub 2021 Jun 2.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

As the application of graphene nanomaterials gets increasingly attractive in the field of tissue engineering and regenerative medicine, the long-term evaluation is necessary and urgent as to their biocompatibility and regenerative capacity in different tissue injuries, such as nerve, bone, and heart. However, it still remains controversial about the potential biological effects of graphene on neuronal activity, especially after severe nerve injuries. In this study, we establish a lengthy peripheral nerve defect rat model and investigate the potential toxicity of layered graphene-loaded polycaprolactone scaffold after implantation during 18 months in vivo. Read More

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Evaluation of osteochondral-like tissues using human freeze-dried cancellous bone and chondrocyte sheets to treat osteochondral defects in rabbits.

Biomater Sci 2021 Jul 21;9(13):4701-4716. Epub 2021 May 21.

Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand. and Department of Chemical Engineering, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.

Human freeze-dried cancellous bone combined with human chondrocyte sheets have recently been used to construct an osteochondral-like tissue, which resembled a cartilage layer on a subchondral bone layer. Nevertheless, the efficacy of these human tissues in a xenogeneic model has been rarely reported. Therefore, this study aimed to evaluate the potential of human freeze-dried cancellous bones combined with human chondrocyte sheets for the treatment of osteochondral defects in rabbits. Read More

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Fabrication of dual layered biocompatible herbal biopatch from biological waste for skin - tissue regenerative applications.

Int J Biol Macromol 2021 Jul 10;183:1106-1118. Epub 2021 May 10.

Department of Biomedical Engineering, K.P.R. Institute of Engineering and Technology, Coimbatore 641407, India.

A dual layered herbal biopolymeric patch (biopatch) with enhanced wound healing efficiency and skin mimicking functions was fabricated for skin-tissue regenerative applications. In this study, hoof keratin (KE) extracted from biological waste and gelatin (GE) was employed for KE-GE biosheet fabrication using a simple casting method. Further, the top layer of the fabricated KE-GE biosheet was coated with bioactive Matricaria recutita (Chamomile flower) extract (CH) with gelatin through an electrospraying method. Read More

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MgFe-LDH Nanoparticles: A Promising Leukemia Inhibitory Factor Replacement for Self-Renewal and Pluripotency Maintenance in Cultured Mouse Embryonic Stem Cells.

Adv Sci (Weinh) 2021 05 25;8(9):2003535. Epub 2021 Feb 25.

Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Orthopaedic Department of Tongji Hospital School of Life Science and Technology Tongji University 389 Xincun Road Shanghai 200065 P. R. China.

Leukemia inhibitory factor (LIF), an indispensable bioactive protein that sustains self-renewal and pluripotency in stem cells, is vital for mouse embryonic stem cell (mESC) culture. Extensive research is conducted on reliable alternatives for LIF as its clinical application in stable culture and large-scale expansion of ESCs is limited by its instability and high cost. However, few studies have sought to replace LIF with nanoparticles to provide a xeno-free culture condition. Read More

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Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study.

BMC Mol Cell Biol 2021 May 10;22(1):28. Epub 2021 May 10.

Department of Physiology and Biochemistry and Centre of Molecular Medicine and Biobanking, Faculty of Medicine and Surgery, University of Malta, Msida, MSD2080, Malta.

Background: Chronic leg ulcerations are associated with Haemoglobin disorders, Type2 Diabetes Mellitus, and long-term venous insufficiency, where poor perfusion and altered metabolism develop into a chronic inflammation that impairs wound closure. Skin equivalent organotypic cultures can be engineered in vitro to study skin biology and wound closure by modelling the specific cellular components of the skin. This study aimed to develop a novel bioactive platelet-rich plasma (PRP) leukocyte depleted scaffold to facilitate the study of common clinical skin wounds in patients with poor chronic skin perfusion and low leukocyte infiltration. Read More

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Preparation and evaluation of an innovative antibacterial bi-layered composite dressing for skin wound healing.

J Tissue Viability 2021 Aug 30;30(3):454-461. Epub 2021 Apr 30.

Weihai Food and Drug Inspection Testing Center, No.52, Xin Wei Road, Weihai, Shandong Province, 264200, PR China.

Aim Of The Study: The aim of the current study was to develop collagen-based bi-layered composite dressings with antibacterial property and evaluate the efficiency for wound healing.

Materials And Methods: A bi-layered composite wound dressing was fabricated using two marine biomacromolecules (collagen and chitosan or carboxymethyl chitosan). Non-crosslinked and N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/N-Hydroxy succinimide (EDC/NHS) cross-linked collagen sponges fabricated by vacuum freeze-drying technology was used as the inner layer. Read More

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Transvaginal primary layered repair of postsurgical urethrovaginal fistula.

Int Urogynecol J 2021 Jul 5;32(7):1941-1943. Epub 2021 May 5.

ASST Monza, Ospedale San Gerardo, via G.B. Pergolesi, 33 20900, Monza, Italy.

Introduction And Hypothesis: Urethrovaginal fistula (UVF) is a rare disorder, which implies the presence of an abnormal communication between the urethra and the vagina.

Methods: Surgical repair options include transurethral, transabdominal and transvaginal procedures, either with or without tissue interposition. The vaginal route is considered a safe and effective option to correct UVF. Read More

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Biomechanical and Mechanostat analysis of a titanium layered porous implant for mandibular reconstruction: The effect of the topology optimization design.

Mater Sci Eng C Mater Biol Appl 2021 May 24;124:112056. Epub 2021 Mar 24.

College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310023, China; Key Laboratory of Special Purpose Equipment and Advanced Processing Technology, Ministry of Education and Zhejiang Province, Zhejiang University of Technology, Hangzhou 310023, China; National International Joint Research Center of Special Purpose Equipment and Advanced Processing Technology, Zhejiang University of Technology, Hangzhou 310023, China.

A porous scaffold/implant is considered a potential method to repair bone defects, but its mechanical stability and biomechanics during the repair process are not yet clear. A mandibular titanium implant was proposed and designed with layered porous structures similar to that of the bone tissue, both in structure and mechanical properties. Topology was used to optimize the design of the porous implant and fixed structure. Read More

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Fabrication of Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model.

Biomacromolecules 2021 05 26;22(5):1885-1900. Epub 2021 Apr 26.

Centre for Biomedical Engineering, Indian Institute of Technology, Delhi, New Delhi 110016, India.

Diabetes mellitus (DM)-associated impairments in wound healing include prolonged inflammation, the overexpression of matrix metalloproteases (MMPs), and low levels of growth factors at the wound site. To this end, a layer-by-layer scaffold (S) made of cross-linked silk fibroin and hyaluronic acid is developed to deliver chlorhexidine, an antimicrobial agent and an MMP-9 inhibitor, along with the PDGF-BB protein. S exhibited highly porous morphology. Read More

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3D Silk Fiber Construct Embedded Dual-Layer PEG Hydrogel for Articular Cartilage Repair - Assessment.

Front Bioeng Biotechnol 2021 24;9:653509. Epub 2021 Mar 24.

Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul, South Korea.

Since articular cartilage does not regenerate itself, researches are underway to heal damaged articular cartilage by applying biomaterials such as a hydrogel. In this study, we have constructed a dual-layer composite hydrogel mimicking the layered structure of articular cartilage. The top layer consists of a high-density PEG hydrogel prepared with 8-arm PEG and PEG diacrylate using thiol-norbornene photo-click chemistry. Read More

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A competitive nature-derived multilayered scaffold based on chitosan and alginate, for full-thickness wound healing.

Carbohydr Polym 2021 Jun 8;262:117921. Epub 2021 Mar 8.

Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman. Electronic address:

The aim of this study was to evaluate a bioactive multilayer wound dressing, based on chitosan and alginate. To enhance healing potential, Dracaena Cinnabari and Aloe Vera were loaded as separate layers into the scaffold. The bare and bioactive multilayered scaffolds were fabricated by an iterative layering freeze-drying technique. Read More

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[Application of three-staged paramedian forehead flap in reconstruction and repair of full-thickness nasal defect].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Apr;56(4):374-380

Department of Maxillofacial Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.

To evaluate the application of three-staged paramendian forehead flap technique in reconstruction of severe full-thickness nasal defect. Clinical data of 7 cases with nasal reconstruction by three-staged forehead flap technique in the First Affiliated Hospital of Xinjiang Medical University and HongKong University Shenzhen Hospital between June 2016 and October 2019 was retrospectively reviewed. All were males aged from 10 to 71 years. Read More

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A Cost-Effective Task Trainer for Complex Lip Laceration Repair.

Cureus 2021 Mar 2;13(3):e13659. Epub 2021 Mar 2.

Department of Emergency Medicine, Vanderbilt University Medical Center, Nashville, USA.

Facial laceration repair is a common emergency department procedure with important cosmetic implications for patients. In instances where the vermillion border is violated special attention must be paid to accurate opposition, as little as 1 mm of misalignment can result in poor cosmetic results. We sought to construct and evaluate an affordable, effective, and easily reproduced simulation trainer of full-thickness lip laceration requiring vermillion border repair primarily for Emergency Medicine resident education. Read More

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Nanotechnology, and scaffold implantation for the effective repair of injured organs: An overview on hard tissue engineering.

J Control Release 2021 05 3;333:391-417. Epub 2021 Apr 3.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

The tissue engineering of hard organs and tissues containing cartilage, teeth, and bones is a widely used and rapidly progressing field. One of the main features of hard organs and tissues is the mineralization of their extracellular matrices (ECM) to enable them to withstand pressure and weight. Recently, a variety of printing strategies have been developed to facilitate hard organ and tissue regeneration. Read More

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