19,652 results match your criteria layer cultures

Matrigel® enhances 3T3-L1 cell differentiation.

Adipocyte 2021 Dec;10(1):361-377

Department of Pediatrics and the Graduate Program in Medical Sciences, McMaster University, Hamilton, ON, Canada.

Culturing cells on bio-gels are believed to provide a more -like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene surfaces. Growth on a 250-μm thick layer of Matrigel® facilitated the formation of cellular aggregates of 3T3-L1 cells. Read More

View Article and Full-Text PDF
December 2021

Enhenced cell adhesion on collagen I treated parylene-C microplates.

J Biomater Sci Polym Ed 2021 Jul 21:1-12. Epub 2021 Jul 21.

Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, PR China.

On account of unique mechanical property and inertia, parylene-C has become a promising material for microdevices especially in three-dimensional microstructures loaded with cells. However, parylene-C is not favorable for cell adhesion, and a routine procedure is to modify it with a new adhesive layer. Herein, the parylene-C substrates with or without collagen I (Col-I) coating were adopted to estimate the influence of micro-environment change on cell attachment and spreading. Read More

View Article and Full-Text PDF

Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA.

Bio Protoc 2018 Jun 20;8(12):e2879. Epub 2018 Jun 20.

Institute for Molecular Infection Biology (IMIB), University of Wuerzburg, Wuerzburg, Germany.

The mucosal surfaces of the gastrointestinal, respiratory, reproductive, and urinary tracts, and the surface of the eye harbor a resident microflora that lives in symbiosis with their host and forms a complex ecosystem. The protection of the vulnerable epithelium is primarily achieved by mucins that form a gel-like structure adherent to the apical cell surface. This mucus layer constitutes a physical and chemical barrier between the microbial flora and the underlying epithelium. Read More

View Article and Full-Text PDF

Facile Route for 3D Printing of Transparent PETg-Based Hybrid Biomicrofluidic Devices Promoting Cell Adhesion.

ACS Biomater Sci Eng 2021 Jul 20. Epub 2021 Jul 20.

Regenerative Medicine and Stem Cell Laboratory (RMS), Department of Biomedical Engineering, Indian Institute of Technology Hyderabad (IITH), Kandi, Sangareddy 502285, Telangana, India.

3D printing has emerged as a promising fabrication technique for microfluidic devices, overcoming some of the challenges associated with conventional soft lithography. Filament-based polymer extrusion (popularly known as fused deposition modeling (FDM)) is one of the most accessible 3D printing techniques available, offering a wide range of low-cost thermoplastic polymer materials for microfluidic device fabrication. However, low optical transparency is one of the significant limitations of extrusion-based microfluidic devices, rendering them unsuitable for cell culture-related biological applications. Read More

View Article and Full-Text PDF

New method for the protection and restoration of calcareous cultural heritage stones by polyelectrolytes and hydroxyapatite nanocrystals.

J Colloid Interface Sci 2021 Jul 6;604:604-615. Epub 2021 Jul 6.

Climate and Atmosphere Research Center, The Cyprus Institute, Nicosia 2121, Cyprus; Faculty of Civil Engineering and Geosciences, Delft University of Technology, Delft 2628 CN, the Netherlands.

We have investigated the feasibility of a new two-step protocol for the restoration of marbles. The process employs a polyelectrolyte multilayer film that enhances the chemical affinity between the treated stone and restorative material (hydroxyapatite nanocrystals), through functionalization, while at the same time it attributes an acid resistant property to the resulting system. Surface functionalization and material deposition is achieved through spraying; a simple and versatile application method suitable for objects of various sizes and geometries. Read More

View Article and Full-Text PDF

Hyaluronic acid-functionalized poly-lactic acid (PLA) microfibers regulate vascular endothelial cell proliferation and phenotypic shape expression.

Colloids Surf B Biointerfaces 2021 Jul 7;206:111970. Epub 2021 Jul 7.

Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China.

This work was designed to evaluate the efficacy of hyaluronic acid (HA) functionalized tubular poly-lactic acid (PLA) microfibers in directing the luminal pre-endothelialization of vascular endothelial cells (ECs). Tubular HA/PLA microfibers with hierarchical architecture were prepared by electrospinning and chemical cross-linking process. A layer of HA microfibrous film coating was fixed on the inner wall surface of the tubular HA/PLA microfibers, resulting in higher anisotropy wettability and relatively lower surface energy and roughness. Read More

View Article and Full-Text PDF

Fabrication and Characterization of 3D Printed, 3D Microelectrode Arrays for Interfacing with a Peripheral Nerve-on-a-Chip.

ACS Biomater Sci Eng 2021 Jul 10;7(7):3018-3029. Epub 2020 Dec 10.

NanoScience Technology Center (NSTC), University of Central Florida, Orlando, Florida 32816, United States.

We present a nontraditional fabrication technique for the realization of three-dimensional (3D) microelectrode arrays (MEAs) capable of interfacing with 3D cellular networks . The technology uses cost-effective to fabricate the 3D MEAs with 3D printed base structures with the metallization of the microtowers and conductive traces being performed by stencil mask evaporation techniques. A biocompatible lamination layer insulates the traces for realization of 3D microtower MEAs (250 μm base diameter, 400 μm height). Read More

View Article and Full-Text PDF

Doping gadolinium versus lanthanum into hydroxyapatite particles for better biocompatibility in bone marrow stem cells.

Chem Biol Interact 2021 Jul 15:109579. Epub 2021 Jul 15.

Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, PR China. Electronic address:

Lanthanide ions (Ln) doped hydroxyapatite (HAP) particles are well established in biomedical areas. Although Ln elements are closely located in the periodic table and have plenty of similar characteristics, the minor differences in the effective ionic radii could cause alterations in the physicochemical and biological properties of HAP substitutes. The present study synthesized lanthanum-(La-) and gadolinium-(Gd-) doped HAP particles (La-HAP and Gd-HAP). Read More

View Article and Full-Text PDF

Isolation and long-term expansion of murine epidermal stem-like cells.

PLoS One 2021 16;16(7):e0254731. Epub 2021 Jul 16.

Department of Environmental and Public Health Sciences, College of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of America.

Epidermis is the most outer layer of the skin and a physical barrier protecting the internal tissues from mechanical and environmental insults. The basal keratinocytes, which, through proliferation and differentiation, supply diverse cell types for epidermal homeostasis and injury repair. Sustainable culture of murine keratinocyte, however, is a major obstacle. Read More

View Article and Full-Text PDF

A human-computer collaboration for COVID-19 differentiation: combining a radiomics model with deep learning and human auditing.

Ann Palliat Med 2021 Jul 9. Epub 2021 Jul 9.

Department of Radiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: This study aimed to build a radiomics model with deep learning (DL) and human auditing and examine its diagnostic value in differentiating between coronavirus disease 2019 (COVID-19) and community-acquired pneumonia (CAP).

Methods: Forty-three COVID-19 patients, whose diagnoses had been confirmed with reverse-transcriptase polymerase-chain-reaction (RT-PCR) tests, and 60 CAP patients, whose diagnoses had been confirmed with sputum cultures, were enrolled in this retrospective study. The candidate regions of interest (ROIs) on the computed tomography (CT) images of the 103 patients were determined using a DL-based segmentation model powered by transfer learning. Read More

View Article and Full-Text PDF

Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System.

ACS Appl Mater Interfaces 2021 Jul 14. Epub 2021 Jul 14.

Graduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan 333, Taiwan.

Hypoxia is a nonphysiological oxygen tension which is common in most malignant tumors. Hypoxia stimulates complicated cell signaling networks in cancer cells, e.g. Read More

View Article and Full-Text PDF

HtrA4 is up-regulated during trophoblast syncytialization and BeWo cells fail to syncytialize without HtrA4.

Sci Rep 2021 Jul 13;11(1):14363. Epub 2021 Jul 13.

Implantation and Placental Development Laboratory, Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, 3168, Australia.

The outer layer of the human placenta comprises syncytiotrophoblast, which forms through fusion of cytotrophoblasts (syncytialization), and plays a critical role in maternal-fetal communication including nutrient/oxygen transportation and hormone secretion. Impairment in syncytialization inevitably affects pregnancy outcomes. High temperature requirement factor A 4 (HtrA4) is a placental-specific protease, expressed by various trophoblasts including syncytiotrophoblast, and significantly elevated in preeclampsia at disease presentation. Read More

View Article and Full-Text PDF

Influence of the mesenchymal stromal cell source on the hematopoietic supportive capacity of umbilical cord blood-derived CD34-enriched cells.

Stem Cell Res Ther 2021 Jul 13;12(1):399. Epub 2021 Jul 13.

Department of Bioengineering and iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Background: Umbilical cord blood (UCB) is a clinically relevant alternative source of hematopoietic stem/progenitor cells (HSPC). To overcome the low cell number per UCB unit, ex vivo expansion of UCB HSPC in co-culture with mesenchymal stromal cells (MSC) has been established. Bone marrow (BM)-derived MSC have been the standard choice, but the use of MSC from alternative sources, less invasive and discardable, could ease clinical translation of an expanded CD34 cell product. Read More

View Article and Full-Text PDF

Multilayered Cell Sheets of Cardiac Reprogrammed Cells for the Evaluation of Drug Cytotoxicity.

Tissue Eng Regen Med 2021 Jul 12. Epub 2021 Jul 12.

School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea.

Background: Various cell-culture systems have been used to evaluate drug toxicity in vitro. However, factors that affect cytotoxicity outcomes in drug toxicity evaluation systems remain elusive. In this study, we used multilayered sheets of cardiac-mimetic cells, which were reprogrammed from human fibroblasts, to investigate the effects of the layer number on drug cytotoxicity outcomes. Read More

View Article and Full-Text PDF

A Continuous Battle for Host-Derived Glycans Between a Mucus Specialist and a Glycan Generalist and .

Front Microbiol 2021 24;12:632454. Epub 2021 Jun 24.

Laboratory of Microbiology, Wageningen University, Wageningen, Netherlands.

The human gastrointestinal tract is colonized by a diverse microbial community, which plays a crucial role in human health. In the gut, a protective mucus layer that consists of glycan structures separates the bacteria from the host epithelial cells. These host-derived glycans are utilized by bacteria that have adapted to this specific compound in the gastrointestinal tract. Read More

View Article and Full-Text PDF

Luminescence chronology for the Paleolithic site of Xinmiaozhuang Locality 1 (XMZ1) in the Nihewan Basin, northern China, and its paleoenvironmental and archaeological implications.

J Hum Evol 2021 Jul 6;157:103033. Epub 2021 Jul 6.

Hebei Provincial Institute of Cultural Relics and Archaeology, Shijiazhuang, 050031, China.

In contrast to the prevailing view that the Chinese Paleolithic has been dominated by the Mode 1 technology-with a slow and conservative development from the Early to the Late Pleistocene-recent discoveries indicate that the lithic technology might have developed into an 'advanced' phase in some parts of China, at least since the early Late Pleistocene. The Xinmiaozhuang Locality 1 (XMZ1), located on the southern edge of the Nihewan Basin in northern China, is one of the examples belonging to such an 'advanced' phase. Although the stone artifacts at this site still belong to the long-existing 'small-tool' industry (core-and-flake) in this basin, some 'advanced' traits, including discoidal cores, elongated flakes, and 'Mousterian-like' triangular points and scrapers, are present. Read More

View Article and Full-Text PDF

Spatially-resolved pharmacokinetic/pharmacodynamic modelling of bystander effects of a nitrochloromethylbenzindoline hypoxia-activated prodrug.

Cancer Chemother Pharmacol 2021 Jul 10. Epub 2021 Jul 10.

Auckland Cancer Society Research Centre, University of Auckland, 85 Park Rd, Grafton, Auckland, 1023, New Zealand.

Purpose: Hypoxia-activated prodrugs (HAPs) have the potential for eliminating chemo- and radiation-resistant hypoxic tumour cells, but their activity is often compromised by limited penetration into hypoxic zones. Nitrochloromethylbenzindoline (nitroCBI) HAPs are reduced in hypoxic cells to highly cytotoxic DNA minor groove alkylating aminoCBI metabolites. In this study, we investigate whether a lead nitroCBI, SN30548, generates a significant bystander effect through the diffusion of its aminoCBI metabolite and whether this compensates for any diffusion limitations of the prodrug in tumour tissue. Read More

View Article and Full-Text PDF

Valorization of date juice by the production of lipopeptide biosurfactants by a Bacillus mojavensis BI2 strain: bioprocess optimization by response surface methodology and study of surface activities.

Bioprocess Biosyst Eng 2021 Jul 9. Epub 2021 Jul 9.

Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisie.

Lipopeptides biosurfactants (BioS) are natural surface-active compounds produced by a variety of microorganisms. They have great interest in environmental, biomedical and agro-industrial fields. However their large-scale application and production is limited by the cost of culture media and the low yield of production. Read More

View Article and Full-Text PDF

[Directed differentiation of porcine induced pluripotent stem cells into forebrain GABAergic neuron progenitors].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jun;41(6):820-827

School of Laboratory Medicine Bengbu Medical College, Bengbu 233000, China.

Objective: To establish an efficient protocol for directed differentiation of miniature-swine induced pluripotent stem cells (iPSCs) into GABAergic progenitors in a chemically defined system.

Objective: We adopted a two-stage protocol for inducing the differentiation of porcine iPSCs. In the first stage, embryoid bodies (EBs) derived from porcine iPSCs after 3 days of suspension culture were induced in neural induction medium (containing SB431542, DMH1 and FGF2) till day 12 to differentiate into primitive neuroepithelia cells (NECs). Read More

View Article and Full-Text PDF

Mesenchymal Stem Cells (MSCs) in Targeted Drug Delivery: Literature Review and Exploratory Data on Migrating and Differentiation Capacities of Bone MSCs into Hepatic Progenitor Cells.

Curr Top Med Chem 2021 Jul 7. Epub 2021 Jul 7.

Department of Surgical Oncology, Shaanxi Provincial People's Hospital (Third Hospital of Medical College of Xi'an Jiaotong University), Xi'an 710068, China.

Background And Objective: Mesenchymal stem cells (MSCs), particularly bone MSCs (BMSCs) offer great potentials for targeted therapeutic applications due to their migratory and differentiation capacities. Significant advances have been achieved in the differentiation of hepatocyte or hepatocyte-like cells both in vitro and in vivo. However, there is limited knowledge on the differentiation of BMSCs into bipotential hepatic progenitor cells or cholangiocytes. Read More

View Article and Full-Text PDF

Antiretroviral Tenofovir Induces Senescence-Associated β-Galactosidase Activity in Primary Human Brain Vascular Cells in Multi-Layer Three-Dimensional Co-Culture.

Cureus 2021 May 30;13(5):e15327. Epub 2021 May 30.

Psychiatry, University of California San Diego, La Jolla, USA.

Introduction In the current context of early diagnosis of HIV infection, immediate initiation of antiretroviral (ARV) therapy, and lifelong chronic treatment, the potential ARV toxicity is of particular concern. Emtricitabine (FTC) and tenofovir (TFV) are commonly used as backbone drugs in ARV regimens recommended for initial therapy of HIV infection. Here we assessed the effects of FTC and TFV exposure on senescence-associated β-galactosidase (SA-β-Gal) activity, a marker of cellular senescence, in human brain vascular cells. Read More

View Article and Full-Text PDF

A novel engineered vascular construct of stem cell-laden 3D-printed PGSA scaffold enhances tissue revascularization.

Biofabrication 2021 Jul 7. Epub 2021 Jul 7.

Institute of Cellular and System Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli County, 35053, TAIWAN.

Development of transplantable engineered tissue has been hampered by lacking vascular network within the engineered tissue. Three-dimensional (3D) printing has emerged as a new technology with great potential in fabrication and customization of geometric microstructure. In this study, utilizing digital light processing system, we manufactured a recently designed novel 3D architecture scaffold with poly(glycerol sebacate) acrylate (PGSA). Read More

View Article and Full-Text PDF

Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells.

Sci Rep 2021 Jul 6;11(1):13893. Epub 2021 Jul 6.

Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, 31116, Republic of Korea.

Several studies have shown how different cell lines can influence the differentiation of stem cells through co-culture systems. The House Ear Institute-Organ of Corti 1 (HEI-OC1) is considered an important cell line for in vitro auditory research. However, it is unknown if HEI-OC1 cells can promote the differentiation of embryonic stem cells (ESCs). Read More

View Article and Full-Text PDF

The Presence or Absence of Alkaline Phosphatase Activity to Discriminate Pluripotency Characteristics in Porcine Epiblast Stem Cell-Like Cells.

Cell Reprogram 2021 Jul 2. Epub 2021 Jul 2.

Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.

Porcine embryonic stem cells (pESCs) would provide potentials for agricultural- and biotechnological-related applications. However, authentic pESCs have not been established yet because standards for porcine stem cell-specific markers and culture conditions are not clear. Therefore, the present study reports attempts to derive pluripotent epiblast stem cells either from or derived porcine embryos. Read More

View Article and Full-Text PDF

Core-shell PLA/Kef hybrid scaffolds for skin tissue engineering applications prepared by direct kefiran coating on PLA electrospun fibers optimized via air-plasma treatment.

Mater Sci Eng C Mater Biol Appl 2021 Aug 10;127:112248. Epub 2021 Jun 10.

Department of Engineering, University of Palermo, RU INSTM, Viale delle Scienze, Palermo, Italy; ATeN Center, University of Palermo, Viale delle Scienze, Palermo, Italy.

Over the recent years, there is a growing interest in electrospun hybrid scaffolds composed of synthetic and natural polymers that can support cell attachment and proliferation. In this work, the physical and biological properties of polylactic acid (PLA) electrospun mats coated with kefiran (Kef) were evaluated. Gravimetric, spectroscopic (FTIR-ATR) and morphological investigations via scanning electron microscopy confirmed the effective formation of a thin kefiran layer wrapped on the PLA fibers with an easy-tunable thickness. Read More

View Article and Full-Text PDF

Transcriptome Dynamics of Epidermal Reprogramming during Direct Shoot Regeneration in Torenia fournieri.

Plant Cell Physiol 2021 Jul 5. Epub 2021 Jul 5.

Botanical Gardens, Graduate School of Science, The University of Tokyo, 3-7-1 Hakusan, Bunkyo-ku, Tokyo 112-0001, Japan.

Shoot regeneration involves reprogramming of somatic cells and de novo organization of shoot apical meristems (SAMs). In the best-studied model system of shoot regeneration using Arabidopsis, regeneration occurs mediated by auxin-responsive pluripotent callus formation from pericycle or pericycle-like tissues according to the lateral root development pathway. In contrast, shoot regeneration can be induced directly from fully differentiated epidermal cells of stem explants of Torenia fournieri (Torenia) without intervening callus mass formation in culture with cytokinin, yet its molecular mechanisms remain unaddressed. Read More

View Article and Full-Text PDF

Therapeutic effect of autologous concentrated growth factor on lower-extremity chronic refractory wounds: A case report.

World J Clin Cases 2021 Jun;9(18):4797-4802

Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, Shenzhen 518110, Guangdong Province, China.

Background: Management of chronic refractory wounds is one of the toughest clinical challenges for surgeons. Because of poor blood supply, less tissue coverage, and easy exposure, the lower leg is a common site for chronic refractory wounds. The current therapeutic regimens often lead to prolonged hospital stay and higher healthcare costs. Read More

View Article and Full-Text PDF

Isolation and Characterization of an Endophytic Fungus Producing Tyrosol From Thunb. Using ITS2 RNA Secondary Structure and Molecular Docking Study.

Front Bioeng Biotechnol 2021 17;9:650247. Epub 2021 Jun 17.

Mycology and Plant Pathology Laboratory, Department of Botany, Gauhati University, Guwahati, India.

An endophytic fungus isolated from healthy leaf tissues of Thunb., an ethnomedicinal plant of North East India, showed a considerable amount of antimicrobial activity. The ethyl acetate extract of the fungal culture filtrates displayed promising antimicrobial activity against a panel of clinically significant pathogens including , , and . Read More

View Article and Full-Text PDF

Vitamin D Deficiency Is Common in Ghana despite Abundance of Sunlight: A Multicentre Comparative Cross-Sectional Study.

J Nutr Metab 2021 10;2021:9987141. Epub 2021 Jun 10.

Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Background: Vitamin D is a steroid hormone important for the normal functioning of the body. It is produced through skin exposure to sunlight and from the diet. Although Ghana is located in the tropics where sunlight is abundant, factors like culture, diet, skin pigmentation, variation in the ozone layer, and geographical area influence the optimization of vitamin D concentration. Read More

View Article and Full-Text PDF