2,108 results match your criteria larval hatching

Lack of negative effects of fasting of gilthead seabream (Sparus aurata) breeders during the spawning period on maternal and egg nutrient composition, fertilization success, and early embryo/larval development.

Fish Physiol Biochem 2021 Aug 5;47(4):1257-1270. Epub 2021 Jul 5.

Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, P.O. Box 2214, Crete, 71003, Heraklion, Greece.

The effect of fasting on spawning performance, maternal, and egg nutrient composition, and on embryo/larval development was monitored in gilthead seabream (Sparus aurata). Two broodstocks were fasted during two consecutive years, for a period of 43 and 54 days within the spawning season, in a preliminary (year 1, 5-year-old breeders) and the main study (year 2, 6-year-old breeders), respectively. Mean daily fecundity showed a declining trend during fasting in the main study only, while fertilization success was high in both years and it was not affected by fasting, as was hatching and 5-day larval survival. Read More

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The impact of different plant extracts on biological parameters of Housefly [ (Diptera: Muscidae)]: Implications for management.

Saudi J Biol Sci 2021 Jul 2;28(7):3880-3885. Epub 2021 Apr 2.

Department of Agronomy, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Pakistan.

Housefly is a significant domestic pest, which causes nuisance. The use of insecticides is discouraged to manage housefly; therefore, alternative management strategies are inevitable. The current study investigated the impact of different plant extracts, i. Read More

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In vitro anthelmintic evaluation of three alkaloids against gastrointestinal nematodes of goats.

Vet Parasitol 2021 Aug 24;296:109505. Epub 2021 Jun 24.

Departamento de Saúde, Universidade Estadual de Feira de Santana, Av.Transnordestina s/n, 44036-900, Feira de Santana, BA, Brazil. Electronic address:

This study assessed the in vitro anthelmintic activity of the alkaloids berberine, harmaline and piperine on gastrointestinal nematodes (GIN) of goat and their possible cytotoxic effects in Vero cells. The anthelmintic evaluation was performed using the egg hatch (EHA) and larval motility (LMA) assays. Cytotoxicity was determined using the 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) assay. Read More

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Dietary Curcumin Promotes Gilthead Seabream Larvae Digestive Capacity and Modulates Oxidative Status.

Animals (Basel) 2021 Jun 3;11(6). Epub 2021 Jun 3.

Centro Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.

The larval stage is highly prone to stress due to the ontogenetic and metabolic alterations occurring in fish. Curcumin inclusion in diets has been shown to improve growth by modulating oxidative status, immune response, and/or feed digestibility in several fish species. The aim of the present work was to assess if dietary curcumin could promote marine fish larvae digestive maturation and improve robustness. Read More

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What Goes Wrong during Early Development of Artificially Reproduced European Eel ? Clues from the Larval Transcriptome and Gene Expression Patterns.

Animals (Basel) 2021 Jun 8;11(6). Epub 2021 Jun 8.

Animal Breeding and Genomics, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.

In eels, large variations in larval mortality exist, which would impede the viable production of juvenile glass eels in captivity. The transcriptome of European eel larvae was investigated to identify physiological pathways and genes that show differential regulation between non-viable vs. viable larvae. Read More

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Differences in Life History Traits in Rural vs. Urban Populations of a Specialist Ground Beetle, .

Insects 2021 Jun 10;12(6). Epub 2021 Jun 10.

Department of Agroecology, Aarhus University Flakkebjerg Research Centre, DK-4200 Slagelse, Denmark.

Urbanization is increasing worldwide and causes substantial changes in environmental parameters, generating various kinds of stress on arthropods, with several harmful consequences. We examined a forest specialist ground beetle, in forested habitats to evaluate the changes in four important life history traits between rural and urban populations. Analyzing beetles from the overwintered cohort in their first breeding season, we found no significant differences in body length or body mass between the rural and urban individuals. Read More

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Changes in digestive enzyme activities during the early ontogeny of the South American cichlid (Cichlasoma dimerus).

Fish Physiol Biochem 2021 Aug 25;47(4):1211-1227. Epub 2021 Jun 25.

Departamento de Fisiología, Facultad de Ciencias de La Salud, Universidad de Granada, Campus de Melilla, Calle Santander, 1, C.P. 52005, Melilla, Spain.

Cichlasoma dimerus is a neotropical cichlid that has been used as a biological model for neuroendocrinology studies. However, its culture is problematic in terms of larval feeding to allow having enough fry quantity and quality. Larviculture requires full knowledge about the digestive system and nutrition; therefore, this study was intended to assess the digestive enzymes' changes at different ages during the early ontogeny. Read More

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Trophic transfer and toxicity of heavy metals from dengue mosquito Aedes aegypti to predator dragonfly Tramea cophysa.

Ecotoxicology 2021 Aug 24;30(6):1108-1115. Epub 2021 Jun 24.

Department of Entomology, UCA & ES, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.

Heavy metal pollution in aquatic habitats can be detrimental to both prey and predators in a food web. To investigate the potential for bio-transfer and bioaccumulation of heavy metals between specific trophic levels, 3rd instar larvae of Aedes aegypti were exposed to mercury (Hg), lead (Pb), cadmium (Cd), copper (Cu), and zinc (Zn) for three consecutive generations and fed to dragonfly (Tramea cophysa) nymphs. Exposure to Hg caused the highest mortality in A. Read More

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Morphology of the first three zoeal stages of the deep-sea caridean shrimp Yang, Chan & Kumar, 2018 (Crustacea, Decapoda, Pandalidae).

Zookeys 2021 4;1042:23-34. Epub 2021 Jun 4.

Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202301, Taiwan National Taiwan Ocean University Keelung Taiwan.

The larvae of the deep-sea pandalid shrimp Yang, Chan & Kumar, 2018 were successfully hatched and cultured to the third zoeal stage. The larvae reached the third zoeal stage nine days after hatching at a water temperature of 21 ± 1 °C. Although members of A. Read More

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Anthelmintic activity of aminoalcohol and diamine derivatives against the gastrointestinal nematode Teladorsagia circumcincta.

Vet Parasitol 2021 Aug 12;296:109496. Epub 2021 Jun 12.

Instituto de Ganadería de Montaña, CSIC-Universidad de León, 24346, Grulleros, León, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071, León, Spain. Electronic address:

Gastrointestinal nematodes (GIN) infections are a serious problem in livestock production due to the great economic losses they cause. Their control is increasingly difficult because of the rapid development of drug resistance and the limited number of available drugs. Therefore, this study evaluated 18 aminoalcohol and 16 diamine derivatives against eggs, first and third stage larvae from a susceptible and a resistant isolate of Teladorsagia circumcincta collected from sheep. Read More

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Larval Performance of Amphidromous and Landlocked Atyid Shrimp Species in the Genus under Different Feeding Conditions.

Zool Stud 2020 15;59:e70. Epub 2020 Dec 15.

Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan, Minato, Tokyo 108-8477, Japan. E-mail: (Hamasaki); (Okada); (Nishimoto); (Dan).

Freshwater shrimps in the family Atyidae exhibit two life history traits: 1) amphidromy, with many small embryos hatching into planktotrophic larvae that develop in the sea, and 2) landlocked, with few large embryos hatching into non-feeding lecithotrophic larvae, or facultative lecithotrophic larvae that develop in freshwater. The lecithotrophy of larvae is considered an adaptation to limited food conditions in the freshwater environment. Furthermore, faster development and settlement behaviours that large larvae exhibit are considered adaptations that allow the larvae to stay in or near parental habitats in fast-flowing streams. Read More

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December 2020

Insecticide resistance exerts significant fitness costs in immature stages of Anopheles gambiae in western Kenya.

Malar J 2021 Jun 9;20(1):259. Epub 2021 Jun 9.

Department of Medical Microbiology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.

Background: Despite increasing documentation of insecticide resistance in malaria vectors against public health insecticides in sub-Saharan Africa, there is a paucity of information on the potential fitness costs of pyrethroid resistance in malaria vectors, which is important in improving the current resistant management strategies. This study aimed to assess the fitness cost effects of insecticide resistance on the development and survival of immature Anopheles gambiae from western Kenya.

Methods: Two-hour old, first instar larvae (L1) were introduced and raised in basins containing soil and rainwater in a semi-field set-up. Read More

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Temperature and food sources influence subadult development and blood-feeding response of Culicoides obsoletus (sensu lato) under laboratory conditions.

Parasit Vectors 2021 Jun 5;14(1):300. Epub 2021 Jun 5.

Sciensano, Enzootic, Vector-Borne and Bee Diseases, Groeselenberg 99, 1180, Brussels, Belgium.

Background: Culicoides obsoletus (s.l.) is the most abundant Culicoides species in northern Europe and an important vector of bluetongue virus and Schmallenberg virus. Read More

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Effects of sulfometuron-methyl on zebrafish at early developmental stages.

Ecotoxicol Environ Saf 2021 Sep 1;220:112385. Epub 2021 Jun 1.

Ganzhou Key Laboratory for Drug Screening and Discovery, School of Geography and Environmental Engineering, Gannan Normal University, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Ji'an 343009, Jiangxi, China. Electronic address:

Sulfometuron methyl (SM) is a widely used herbicide and thus leading to accumulation in the environment. The toxicity assessments of SM in model organisms are currently rare. In the present study, zebrafish were utilized for evaluating the detrimental effects of SM in aquatic vertebrates. Read More

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September 2021

Cooling of Siamese fighting fish Betta splendens (Teleostei, Osphronemidae) embryos at low temperatures.

Cryobiology 2021 Jun 1. Epub 2021 Jun 1.

Laboratorio de Ictiofisiología y Acuicultura, Instituto Tecnológico de Chascomús, (CONICET-UNSAM), Intendente Marino Km. 8.200, B7130IWA, Chascomús, Buenos Aires, Argentina. Electronic address:

The Siamese fighting fish (Betta splendens) has great importance as an ornamental aquarium fish as well as laboratory model species. Due to its rapid development, a cooling-embryo protocol could provide some advantages in their transportation, embryonic synchronization, and optimization of hatcheries. In this context, this work aimed to develop a protocol to storage B. Read More

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Genotoxic and mutagenic effects of methyl ether dillapiole on the development of Aedes aegypti (Diptera: Culicidae).

Med Vet Entomol 2021 Jun 2. Epub 2021 Jun 2.

Graduate Program in Genetics, Conservation, and Evolutionary Biology - PPG - GCBEv, Manaus, Amazonas, Brazil.

Dillapiole, extracted from Piper aduncum essential oil and its derivatives, has been shown to be a potential alternative to the control of Aedes aegypti, which has become resistant to synthetic insecticides. Methyl ether dillapiole (MED) and temephos (TM) were compared to complement the data on the genotoxicity and developmental changes of Ae. aegypti. Read More

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Historical control data in ecotoxicology: Eight years of tests with the copepod Acartia tonsa.

Environ Pollut 2021 Sep 27;284:117468. Epub 2021 May 27.

Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy; Italian Institute for Environmental Protection and Research, ISPRA, Via del Cedro, 38, 57123, Livorno, Italy. Electronic address:

The calanoid copepod Acartia tonsa is one of the most frequently used organisms in acute, short-term bioassays to assess effects induced by marine matrices or chemicals on different life stages. Physiological responses in such tests can be highly variable and historical control data (HCD), values recorded from previous studies performed under similar conditions, can be useful to recognise the average responses over time. Here, we analysed egg hatching success and larval (naupliar) immobilisation/mortality of A. Read More

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September 2021

Influence of oceanic and climate conditions on the early life history of European seabass Dicentrarchus labrax.

Mar Environ Res 2021 Jul 14;169:105362. Epub 2021 May 14.

Centre for Functional Ecology - Science for People & the Planet (CFE), Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal.

Understanding how marine fish early-life history is affected in the long-term by environmental and oceanographic factors is fundamental given its importance to population dynamics and connectivity. This work aimed at determining the influence of these processes on the interannual variability in hatch day and early-life growth patterns of European seabass, over a seven-year period (2011-2017) in the Atlantic Iberian coast. To accomplish this, otolith microstructure analysis was used to determine seabass hatch day and to develop early-growth correlations. Read More

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Expression of long-chain polyunsaturated fatty acids biosynthesis genes during the early life-cycle stages of the tropical gar Atractosteus tropicus.

Comp Biochem Physiol B Biochem Mol Biol 2021 May 28;256:110628. Epub 2021 May 28.

Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, C.P.86139 Villahermosa, Tabasco, Mexico; Consejo Nacional de Ciencia y Tecnología, CDMX, Mexico. Electronic address:

Long-chain (≥C) polyunsaturated fatty acids (LC-PUFA), including eicosapentaenoic acid (EPA, 20:5n-3), arachidonic acid (ARA, 20:4n-6) and docosahexaenoic acid (DHA, 22:6n-3), are essential in multiple physiological processes, especially during early development of vertebrates. LC-PUFA biosynthesis is achieved by two key families of enzymes, fatty acyl desaturases (Fads) and elongation of very long-chain fatty acid (Elovl). The present study determined the expression patterns of genes encoding desaturases (fads1 and fads2) and elongases (elovl2 and elovl5) involved in the LC-PUFA biosynthesis during early life-stages of the tropical gar Atractosteus tropicus. Read More

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Sediment from lake with missing egg bank is toxic to hatchlings of model zooplankton: A reason to consider obligate dormancy in toxicological assessment.

Aquat Toxicol 2021 Jul 11;236:105862. Epub 2021 May 11.

Department of Biology and Marine Biology, University of North Carolina at Wilmington, 601 S College Rd., Wilmington, North Carolina 28403, United States. Electronic address:

Over the last 60 years, valuable progress was made in the standardization of environmental monitoring with model zooplankton. However, obligate dormancy in zooplankton life cycles is not yet considered in standardized toxicology methods. Most zooplankton from coastal and inland waters use dormancy as a critical ecological strategy, and exposure to toxicants during dormancy or resurrection from dormancy alters developmental patterning and hatching success. Read More

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Embryonic toxicity of epoxiconazole exposure to the early life stage of zebrafish.

Sci Total Environ 2021 Jul 16;778:146407. Epub 2021 Mar 16.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address:

Epoxiconazole (EPX), as a broad-spectrum triazole fungicide, is widely used in agriculture to resist pests and diseases, while it may have potential toxicity to non-target organisms. In the present study, early developmental stage zebrafish were used as the subject organisms to assess the toxicity of EPX, and the possible mechanism of toxicity was also discussed by biochemical and transcriptomic analysis. Through embryo toxicity test, we had made it clear that the 96 h LC of embryo was 7. Read More

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Protaspid larvae are unique to trilobites.

Arthropod Struct Dev 2021 Jul 22;63:101059. Epub 2021 May 22.

Czech Academy of Sciences, Institute of Geology, Rozvojová 269, 165 00, Prague 6, Czech Republic; Institute of Geology and Palaeontology, Faculty of Science, Charles University, Albertov 6, Prague, 12843, Czech Republic. Electronic address:

Trilobites represent a model for 'evo-devo' studies in fossil euarthropods, owing to a rare developmental trait: the biomineralization of the dorsal exoskeleton soon after hatching. Many fossilized trilobite ontogenies thus feature early stages - the protaspides - characterized by non-articulated, calcified dorsal exoskeletons. The recent discovery of a protaspid-like fossil occurring with aglaspidid remains in Middle Ordovician strata of Siberia has been interpreted as evidence for the presence of protaspides in these distant relatives of trilobites. Read More

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Ontogeny and development of the gastrointestinal system in Indian walking catfish (Clarias magur) during its early development.

Fish Physiol Biochem 2021 Aug 14;47(4):1033-1052. Epub 2021 May 14.

ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India.

Ontogeny of the digestive tract and its accessory organs and their further development in the Indian walking catfish (Clarias magur) were examined in larvae, starting from the day of hatching until 35 days post-hatching (dph) reared at 28-29 °C. Many organs at their primordial stage were seen on the day of hatching. These include opened oral cavity with monolayered epithelial lining and very few newly emerging taste buds and goblet cells, primordial pharyngeal teeth on slightly stratified epithelia of the pharyngeal plate, stomach anlage with some degree of the mucosal fold, and a few newly forming gastric glands embedded under its mucosa, primordial anterior and posterior intestine with the smooth mucosal surface, anal opening, and primordial liver and pancreas. Read More

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Thermal requirements of immature stages of Chrysomya albiceps (Diptera: Calliphoridae) as a common forensically important fly.

Sci Justice 2021 May 25;61(3):227-234. Epub 2021 Feb 25.

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Entomological material may be used to estimate the time since death occurred (postmortem interval, PMI) in forensically obscure cases. The method that is commonly used to calculate minimum post-mortem interval (mPMI, i.e. Read More

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Insecticidal Effect of Phosphine on Eggs of the Khapra Beetle (Coleoptera: Dermestidae).

J Econ Entomol 2021 06;114(3):1389-1400

Institute of Bio-economy and Agri-technology (iBO), Center for Research and Technology, Volos, Magnesia, Greece.

Trogoderma granarium Everts (Coleoptera: Dermestidae) is one of the most important quarantine pests of stored grains. Control of this insect species can be achieved through a gaseous insecticide, phosphine. Many studies focus on the effect of phosphine on different developmental stages of insects, with most of them highlighting eggs as the most tolerant stage. Read More

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Domestication affected stress and immune response markers in Perca fluviatilis in the early larval stage.

Fish Shellfish Immunol 2021 Jul 1;114:184-198. Epub 2021 May 1.

Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland. Electronic address:

It is already known that domestication modifies stress and immune responses in juveniles and adults of several fish species. However, there is a lack of information on whether these modulations result from adaptability along the life cycle or if they are pre-determined in very early developmental stages. To shed light on mechanisms that help to explain the process of domestication, a study was conducted to analyze comparatively Eurasian perch larval performance, stress, and immune status between wild and domesticated specimens. Read More

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Embryonic ethanol exposure on zebrafish early development.

Brain Behav 2021 06 3;11(6):e02062. Epub 2021 May 3.

Physiology and Behavior Department, Federal University of Rio Grande do Norte, Natal, Brazil.

Introduction: Embryonic exposure to ethanol leads to a condition of physical, behavioral, and cognitive deficiencies named fetal alcohol spectrum disorders (FASD). The most severe variations are in fetal alcohol syndrome (FAS), which is easier to diagnose and not studied in animal models. On the other side, the pFAS (partial fetal alcohol syndrome) includes cases of alcohol-related congenital disabilities and neurodevelopmental disorder with an inconclusive diagnosis. Read More

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Larval morphology of three syntopic species of emKaloula/em Gray (Anura: Microhylidae) from Vietnam.

Zootaxa 2021 Apr 8;4952(1):zootaxa.4952.1.4. Epub 2021 Apr 8.

A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, 33, Leninsky prospect, Moscow 117071, Russia Joint Russian-Vietnamese Tropical Research and Technological Centre, Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Vietnam.

The external larval morphology of three species of the microhylid genus Kaloula (K. indochinensis, K. mediolineata, and K. Read More

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Experimental manipulation of cavity temperature produces differential effects on parasite abundances in blue tit nests at two different latitudes.

Int J Parasitol Parasites Wildl 2021 Apr 22;14:287-297. Epub 2021 Mar 22.

Evolutionary Ecology. Museo Nacional de Ciencias Naturales CSIC, Madrid, Spain.

Although different predictive models forecast that climate change will alter the distribution and incidence of parasitic diseases, few studies have investigated how microclimatic changes may affect host-parasite relationships. In this study, we experimentally increased the temperature inside nest boxes of the blue tit during the nestling period at two different latitudes (central Spain and central Germany) to determine its effect on parasite abundance. The two localities have contrasting climate conditions: the southern one in Spain is warmer and drier than the northern one in Germany. Read More

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Sex dimorphism in European sea bass (Dicentrarchus labrax L.): New insights into sex-related growth patterns during very early life stages.

PLoS One 2021 22;16(4):e0239791. Epub 2021 Apr 22.

MARBEC, CNRS, Ifremer, IRD, Univ Montpellier, Palavas-les-Flots, France.

The European sea bass (Dicentrarchus labrax) exhibits female-biased sexual size dimorphism (SSD) early in development. New tagging techniques provide the opportunity to monitor individual sex-related growth during the post-larval and juvenile stages. We produced an experimental population through artificial fertilization and followed a rearing-temperature protocol (~16°C from hatching to 112 days post-hatching, dph; ~20°C from 117 to 358 dph) targeting a roughly balanced sex ratio. Read More

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