5,533 results match your criteria lambs pcr

Echinococcus granulosus sensu stricto G1 is the predominant genotype in human and livestock isolates from Turkey and Iran, based on mitochondrial nad5 gene differentiation.

Parasit Vectors 2021 Jul 20;14(1):369. Epub 2021 Jul 20.

Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.

Background: Different genotypes of Echinococcus granulosus sensu stricto (s.s.) isolated from livestock and humans have been identified based on cox1 and nad1 genomic fragments. Read More

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Cloning, Expression and Serodiagnostic Potential of HSP70 of Taenia multiceps in Sheep.

Mol Biochem Parasitol 2021 Jul 17:111397. Epub 2021 Jul 17.

Department of Parasitology, Faculty of Veterinary Medicine, University of Bingöl, Bingöl, Turkey.

The aim of this study was to determine the efficacy of the recombinant protein expressed by the T. multiceps HSP70 gene in the immunodiagnosis of sheep coenurosis. Specific primers were designed to amplify the gene encoding HSP70 from the whole genome sequence of T. Read More

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A survey on equine tick-borne diseases: The molecular detection of Babesia ovis DNA in Turkish racehorses.

Ticks Tick Borne Dis 2021 Jul 12;12(5):101784. Epub 2021 Jul 12.

Selcuk University, Faculty of Veterinary Medicine, Department of Parasitology, Konya, Turkey. Electronic address:

Common vector-borne diseases of horses include equine piroplasmosis (EP) caused by Babesia caballi and Theileria equi, and equine granulocytic anaplasmosis (EGA) caused by Anaplasma phagocytophilum. Equine piroplasmosis leads to severe health issues in horses and restrictions on the movement of horses internationally. Anaplasma phagocytophilum causes an acute febrile illness in horses and is also of zoonotic importance. Read More

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Identification and expression analysis of the β-defensin genes in the goat small intestine.

Gene 2021 Jul 15:145846. Epub 2021 Jul 15.

Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, Jiangxi, China.

Defensins represent a family of cysteine-rich peptides that have broad-spectrum antimicrobial activities and serve as a typical kind of effector molecule in the immunity. Ruminant species have a large number of β-defensins in the absence of α- and θ-defensins. It is well-known that the genomes of sheep and cattle harbor at least 43 and 57 β-defensin genes, respectively. Read More

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Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait.

BMC Vet Res 2021 Jul 17;17(1):245. Epub 2021 Jul 17.

Virology lab., Veterinary Laboratories, PAAFR, 1307 Safat,, Rabyia, Farwanyia, Kuwait.

Background: Group A rotaviruses (RVA) are zoonotic pathogens responsible for acute enteritis in human and neonatal ruminants. This research aimed to determine the prevalence of RVA in ruminants (cattle, sheep, and goats) and investigate the circulating RVA genotypes in these animals in Kuwait. We conducted a cross-sectional study to detect RVA in ruminants, using an immunochromatography test (IC), direct sandwich ELISA test, and real-time RT-PCR (RT-qPCR) assay using fecal samples. Read More

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Molecular investigation and phylogeny of Theileria spp. from naturally infected sheep and the first report of Theileria sp. OT3 in Sulaymaniyah governorate/Iraq.

Pol J Vet Sci 2021 Jun;24(2):201-209

Department of Microbiology, College of Medicine, University of Sulaimani, Madame Mitterrand, Sulaymaniyah 46001, Kurdistan Region, Iraq.

Theileriosis is a significant hemoprotozoal disease of domestic and wild ruminants in tropical and subtropical regions of the world. Ovines are mainly infected by T. ovis and T. Read More

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Investigation of the Prevalence of in Southern Xinjiang, China.

J Vet Res 2021 Jun 23;65(2):155-160. Epub 2021 Apr 23.

College of Animal Science, Tarim University, Alar 843300, Xinjiang, People's Republic of China.

Introduction: It is very important to monitor the infection of as a potential threat to the sheep industry. Southern Xinjiang is a major sheep breeding base in China, however, there is no relevant information concerning the infection of the region's ovine stock with this bacteria at present. This study aimed to address this knowledge gap. Read More

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Cattle connection: molecular epidemiology of BVDV outbreaks via rapid nanopore whole-genome sequencing of clinical samples.

BMC Vet Res 2021 Jul 12;17(1):242. Epub 2021 Jul 12.

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493, Greifswald - Insel Riems, Germany.

Background: As a global ruminant pathogen, bovine viral diarrhea virus (BVDV) is responsible for the disease Bovine Viral Diarrhea with a variety of clinical presentations and severe economic losses worldwide. Classified within the Pestivirus genus, the species Pestivirus A and B (syn. BVDV-1, BVDV-2) are genetically differentiated into 21 BVDV-1 and four BVDV-2 subtypes. Read More

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Analysis of environmental dust in goat and sheep farms to assess Coxiella burnetii infection in a Q fever endemic area: Geographical distribution, relationship with human cases and genotypes.

Zoonoses Public Health 2021 Jul 8. Epub 2021 Jul 8.

Department of Animal Health, NEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Derio, Spain.

Real-time PCR analysis of environmental samples (dust and aerosols) is an easy tool to investigate the presence of Coxiella burnetii in the farm environment. The aim of this study was to assess the distribution of C. burnetii DNA in dust collected inside animal premises from 272 small ruminant farms in Bizkaia (northern Spain), a region with recent reports of human Q fever cases and outbreaks. Read More

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Molecular Evidence of in Ticks in Border Areas of Xinjiang, China.

Front Vet Sci 2021 22;8:675457. Epub 2021 Jun 22.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

are gram-negative intracellular bacteria; certain species of can cause diseases in mammals and humans. Ticks play a major role in the transmission of . Xinjiang is the largest province in China according to land area and has one-third of the tick species in China; the infection rate of in ticks in the Xinjiang border areas has not been studied in detail. Read More

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Effect of the Gene on Preadipocyte Differentiation in Sheep.

Front Genet 2021 21;12:649140. Epub 2021 Jun 21.

Institute of Animal Biotechnology, Jilin Academy of Agricultural Science, Changchun, China.

Acetyl-CoA acyltransferase 1 () functions as a key regulator of fatty acid β-oxidation in peroxisomes by catalyzing the cleavage of 3-ketoacyl-CoA to acetyl-CoA and acyl-CoA, which participate in the extension and degradation of fatty acids. Thus, is an important regulator of lipid metabolism and plays an essential role in fatty acid oxidation and lipid metabolism. Our previous study findings revealed that is closely associated with the peroxisome proliferator-activated receptor () signaling and fatty acid metabolism pathways, which are involved in fat deposition in sheep, leading to our hypothesis that may be involved in fat deposition by regulating lipid metabolism. Read More

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Molecular Characterization and Phylogenetic Analysis of Pathogenic Theileria spp. Isolated from Cattle and Sheep based on Cytochrome b Gene in Iran.

Arch Razi Inst 2021 Jul 1;76(2):243-252. Epub 2021 Jul 1.

Department of Parasitic Vaccines Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

The present study investigated the phylogenetic relationship based on cytochrome b gene sequences among pathogenic Theileria species (spp.) in Iran, including Theileria annulata and Theileria lestoquardi, along with other data available in GenBank. A total of 136 (cattle) and 80 (sheep) blood samples suspected of piroplasm infection were obtained from six different provinces of Iran. Read More

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Detection of Fasciola Hepatica in Lori Sheep Using Polymerase Chain Reaction and Conventional Diagnostic Methods in Western Iran.

Arch Razi Inst 2021 Jul 1;76(2):223-229. Epub 2021 Jul 1.

Department of Biotechnology, Faculty of Basic Sciences, Ahvaz Azad University, Ahvaz, Iran.

Fascioliasis is an emerging and important food and water-borne disease in human communities which has become one of the most important health challenges in countries, like Iran. It causes weight loss, a decrease in feed conversion ratio as well as milk and meat production, and also reduces fertility in animals the prevalence of fasciolosis is increasing in some regions of the world due to various factors. Different methods have been used for the detection of Fasciola hepatica in animals. Read More

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Molecular Identification and Genotyping of Theileria Orientalis Type 3 (Buffeli) Isolated from Cattle Using Nested-PCR Assay in Guilan Province, Iran.

Arch Razi Inst 2021 Jul 1;76(2):213-221. Epub 2021 Jul 1.

Veterinarian in Private Clinical Practice, Takistan, Qazvin Province, Iran.

Protozoan parasites of the genus Theileria are tick-borne parasites that have been found in many species of mammals. More than a dozen species of Theileria have been found in cattle, water buffalo, sheep, and goats. Theileria orientalis is a non-pathogenic blood protozoan parasite that was detected and identified during a regular investigation of piroplasmida infection in indigenous cattle in the spring of 2019 in Northern Provinces of Iran. Read More

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A Retrospective Survey of the Abortion Outbreak Event Caused by Brucellosis at a Blue Fox Breeding Farm in Heilongjiang Province, China.

Front Vet Sci 2021 15;8:666254. Epub 2021 Jun 15.

Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China.

Brucellosis is a common zoonosis in China, resulting in abortion in animals. Outbreaks of abortion in blue foxes caused by infection have rarely been reported. In the present study, 3-5 mL blood samples collected from the femoral veins of 10 abortuses of blue foxes were assessed by RBPT (Rose Bengal plate test) and SAT (serum tube agglutination test) to preliminarily investigate the source of infection for the clustering of abortion events at a blue fox farm in Heilongjiang Province. Read More

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Ovine Variation and Its Association With Flystrike Susceptibility.

Front Genet 2021 15;12:675305. Epub 2021 Jun 15.

Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Christchurch, New Zealand.

Flystrike is a major cost and a welfare issue for the New Zealand sheep industry. There are several factors that can predispose sheep to flystrike, such as having fleecerot, a urine-stained breech, and "dags" (an accumulation of fecal matter in the wool of the breech). The FABP4 gene () has been associated with variation in ovine fleecerot resistance, with a strong genetic correlation existing between fleecerot and flystrike occurrence. Read More

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Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC .

Front Immunol 2021 15;12:666543. Epub 2021 Jun 15.

Animal Production and Health Laboratory, Joint FAO/IAEA Agricultural and Biotechnology Laboratory, Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.

Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia and Africa with significant economic losses. SPPV is genetically and immunologically closely related to goatpox virus (GTPV) and lumpy skin disease virus (LSDV), which infect goats and cattle respectively. SPPV live attenuated vaccines (LAVs) are used for vaccination against SPP and goatpox (GTP). Read More

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Ticks and Tick-Borne Pathogens Associated with Dromedary Camels () in Northern Kenya.

Microorganisms 2021 Jun 30;9(7). Epub 2021 Jun 30.

International Centre of Insect Physiology and Ecology (icipe), Nairobi P.O. Box 30772-00100, Kenya.

Ticks and tick-borne pathogens (TBPs) are major constraints to camel health and production, yet epidemiological data on their diversity and impact on dromedary camels remain limited. We surveyed the diversity of ticks and TBPs associated with camels and co-grazing sheep at 12 sites in Marsabit County, northern Kenya. We screened blood and ticks (858 pools) from 296 camels and 77 sheep for bacterial and protozoan TBPs by high-resolution melting analysis and sequencing of PCR products. Read More

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Flock Management Risk Factors Associated with Q Fever Infection in Sheep in Saudi Arabia.

Animals (Basel) 2021 Jun 30;11(7). Epub 2021 Jun 30.

Al Ahsa Veterinary Diagnostic Lab, Ministry of Environment, Water and Agriculture, Al-Ahsa 31982, Saudi Arabia.

Q fever is a zoonotic disease caused by (), an intracellular, Gram-negative bacterium that infects humans and domestic ruminants. Information on flock management factors associated with Q fever seropositivity in Saudi Arabia is very scarce. Therefore, the objective of this study was to identify the animal and flock management factors associated with Q fever seropositivity. Read More

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Whole-Genome Sequencing for Tracing the Genetic Diversity of and Isolated from Livestock in Egypt.

Pathogens 2021 Jun 16;10(6). Epub 2021 Jun 16.

Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany.

Brucellosis is a highly contagious zoonosis that occurs worldwide. Whole-genome sequencing (WGS) has become a widely accepted molecular typing method for outbreak tracing and genomic epidemiology of brucellosis. Twenty-nine spp. Read More

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Cerebrospinal Fluid and Plasma Small Extracellular Vesicles and miRNAs as Biomarkers for Prion Diseases.

Int J Mol Sci 2021 Jun 25;22(13). Epub 2021 Jun 25.

Laboratorio de Genética Bioquímica (LAGENBIO), Instituto Agroalimentario de Aragón (IA2), Instituto de Investigación Sanitaria de Aragón (IISAragón), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.

Diagnosis of transmissible spongiform encephalopathies (TSEs), or prion diseases, is based on the detection of proteinase K (PK)-resistant PrP in post-mortem tissues as indication of infection and disease. Since PrP detection is not considered a reliable method for in vivo diagnosis in most TSEs, it is of crucial importance to identify an alternative source of biomarkers to provide useful alternatives for current diagnostic methodology. Ovine scrapie is the prototype of TSEs and has been known for a long time. Read More

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Synergistic Application of Molecular Markers and Community-Based Microbial Source Tracking Methods for Identification of Fecal Pollution in River Water During Dry and Wet Seasons.

Front Microbiol 2021 14;12:660368. Epub 2021 Jun 14.

State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Beijing, China.

It is important to track fecal sources from humans and animals that negatively influence the water quality of rural rivers and human health. In this study, microbial source tracking (MST) methods using molecular markers and the community-based FEAST (fast expectation-maximization microbial source tracking) program were synergistically applied to distinguish the fecal contributions of multiple sources in a rural river located in Beijing, China. The performance of eight markers were evaluated using 133 fecal samples based on real-time quantitative (qPCR) technique. Read More

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Determining the presence of undeclared animal species using Real-time PCR in canned and ready-to-eat meat products in South Africa.

J Food Sci Technol 2021 Jul 10;58(7):2699-2704. Epub 2020 Sep 10.

Human Molecular Biology Unit, School of Biomedical Sciences, University of the Free State, PO Box 339 (G2), Bloemfontein, 9301 South Africa.

DNA based PCR is the most widely used technique for the detection of animal species in processed meat products. However, the detection of animal species in highly processed meat products, specifically, canned meat, has been reported to be challenging due to the presence of highly degraded DNA and/or the inability to extract sufficient amount of amplifiable DNA. The aim of this study was to evaluate the use of Real-time PCR to detect animal species in ready-to-eat meat products which represent highly processed complex food matrices. Read More

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Multidrug-Resistant Extended-Spectrum β-Lactamase-Producing Pathotypes in North Eastern Region of India: Backyard Small Ruminants-Human-Water Interface.

Microb Drug Resist 2021 Jun 29. Epub 2021 Jun 29.

Central Agricultural University, Imphal, India.

A total of 648 diarrheagenic (DEC) were isolated from calves ( = 219), lambs ( = 87), kids ( = 103), human ( = 193), and water ( = 46) samples. The presence of enteropathogenic (EPEC), enterotoxigenic (ETEC), and shigatoxigenic (STEC) was confirmed by PCR-based detection of the Shiga toxin, intimin, hemolysin, and enterotoxin genes. All the isolates were tested for antimicrobial resistance (AMR) by disc diffusion assay. Read More

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Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles.

Front Genet 2021 11;12:678825. Epub 2021 Jun 11.

College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Background: Inner Mongolian cashmere goats have hair of excellent quality and high economic value, and the skin hair follicle traits of cashmere goats have a direct and important effect on cashmere yield and quality. Circular RNA has been studied in a variety of tissues and cells.

Result: In this study, high-throughput sequencing was used to obtain the expression profiles of circular RNA (circRNA) in the hair follicles of Inner Mongolian cashmere goats at different embryonic stages (45, 55, 65, and 75 days). Read More

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Vaccination with Neospora caninum-cyclophilin and -profilin confers partial protection against experimental neosporosis-induced abortion in sheep.

Vaccine 2021 Jul 24;39(32):4534-4544. Epub 2021 Jun 24.

Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705, United States. Electronic address:

The purpose of this study was to evaluate the protective efficacy of a vaccine consisting of recombinant Neospora caninum-cyclophilin (NcCyP) and -profilin (NcPro) in sheep. At 42 d and 21 d prior to mating, adult Dorset ewes were immunized with the rNcCyP-rNcPro vaccine (Group 1) or co-purifying non-recombinant (NR) control vaccine (Group 2). At 90 days post-mating, all immunized ewes and were challenged by intravenous injection with 10Nesopora caninum Illinois tachyzoites (NcTZ). Read More

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Occurrence, virulence, carbapenem resistance, susceptibility to disinfectants and public health hazard of Pseudomonas aeruginosa isolated from animals, humans and environment in intensive farms.

J Appl Microbiol 2021 Jun 25. Epub 2021 Jun 25.

Animal Health Research Institute, Giza, Egypt.

Aims: This work aimed to determine the occurrence, virulence, antibiogram, carbapenem resistance genes and susceptibility to disinfectants of Pseudomonas aeruginosa isolated from animals, environment and workers in intensive farms.

Methods And Results: A total of 610 samples from intensive beef cattle and sheep farms in Kafr El Sheikh Governorate, Egypt were screened for the presence of P. aeruginosa using bacteriological assays. Read More

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Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan.

J Vet Sci 2021 Jun 3. Epub 2021 Jun 3.

Department of Zoology, Faculty of Sciences, PMAS-Arid Agriculture University, Rawalpindi 46000, Pakistan.

Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2).

Objectives: This study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods.

Methods: Blood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. Read More

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Viral infections shared between water buffaloes and small ruminants in Switzerland.

J Vet Diagn Invest 2021 Jun 24:10406387211027131. Epub 2021 Jun 24.

Institute of Virology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.

Importation of exotic animals that may harbor infectious agents poses risks for native species with potentially severe impacts on animal health and animal production. Although the Asian water buffalo () population in Europe is steadily increasing, its susceptibility to viral infections and its role for interspecies transmission is largely unknown. To identify viral infections that are shared between exotic water buffaloes and native small ruminants, we collected blood samples from 3 Swiss farms on which water buffaloes were kept either without, or together with, sheep or goats. Read More

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Epizootiology and Molecular Identification of Trypanosome Species in Livestock Ruminants in the Gambia.

Acta Parasitol 2021 Jun 23. Epub 2021 Jun 23.

Department of Biochemistry, Ahmadu Bello University, P.M.B 1044, Zaria, Kaduna State, Nigeria.

Introduction: African Animal Trypanosomiasis (AAT) or nagana in animals, is caused by the blood-borne parasitic protozoa called trypanosomes, and is potentially fatal. It is estimated that Africa loses $4‒5 billion annually due to the death of livestock to nagana in the tsetse belt.

Purpose: Although The Gambia lies within this belt, there is scanty data regarding the epizootiology of nagana in The Gambia. Read More

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