8,036 results match your criteria lactococcus


Cyclic di-AMP Oversight of Counter-Ion Osmolyte Pools Impacts Intrinsic Cefuroxime Resistance in Lactococcus lactis.

mBio 2021 04 8;12(2). Epub 2021 Apr 8.

School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland, Australia

The broadly conserved cyclic di-AMP (c-di-AMP) is a conditionally essential bacterial second messenger. The pool of c-di-AMP is fine-tuned through diadenylate cyclase and phosphodiesterase activities, and direct binding of c-di-AMP to proteins and riboswitches allows the regulation of a broad spectrum of cellular processes. c-di-AMP has a significant impact on intrinsic β-lactam antibiotic resistance in Gram-positive bacteria; however, the reason for this is currently unclear. Read More

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Stimulation of regulatory T cells with Lactococcus lactis expressing enterotoxigenic E. coli colonization factor antigen 1 retains salivary flow in a genetic model of Sjögren's syndrome.

Arthritis Res Ther 2021 Apr 6;23(1):99. Epub 2021 Apr 6.

Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, P.O. Box 110880, Gainesville, FL, 32611, United States.

Background: Sjögren's syndrome (SjS), one of the most common autoimmune diseases, impacts millions of people annually. SjS results from autoimmune attack on exocrine (salivary and lacrimal) glands, and women are nine times more likely to be affected than men. To date, no vaccine or therapeutic exists to treat SjS, and patients must rely on lifelong therapies to alleviate symptoms. Read More

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Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ.

Open Biol 2021 Apr 7;11(4):200406. Epub 2021 Apr 7.

Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

The ATP-binding cassette transporter GlnPQ is an essential uptake system that transports glutamine, glutamic acid and asparagine in Gram-positive bacteria. It features two extra-cytoplasmic substrate-binding domains (SBDs) that are linked in tandem to the transmembrane domain of the transporter. The two SBDs differ in their ligand specificities, binding affinities and their distance to the transmembrane domain. Read More

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Proteome constraints reveal targets for improving microbial fitness in nutrient-rich environments.

Mol Syst Biol 2021 04;17(4):e10093

TiFN, Wageningen, the Netherlands.

Cells adapt to different conditions via gene expression that tunes metabolism for maximal fitness. Constraints on cellular proteome may limit such expression strategies and introduce trade-offs. Resource allocation under proteome constraints has explained regulatory strategies in bacteria. Read More

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Heat-Killed Lactic Acid Bacteria Inhibit Nitric Oxide Production via Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cells.

Probiotics Antimicrob Proteins 2021 Apr 5. Epub 2021 Apr 5.

MEDIOGEN, Co., Ltd., Jecheon, 27159, Korea.

Heat-killed lactic acid bacteria perform immunomodulatory functions and are advantageous as probiotics, considering their long product shelf-life, easy storage, and convenient transportation. In this study, we aimed to develop appropriate heat treatments for industrial preparation of probiotics with antioxidant activity. Among 75 heat-killed strains, Lactococcus lactis MG5125 revealed the highest nitric oxide inhibition (86. Read More

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Two ABC transport systems carry out peptide uptake in Enterococcus faecalis: their roles in growth and in uptake of sex pheromones.

Mol Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Dept of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, MN, 55455, USA.

Enterococcal pheromone-inducible plasmids encode a predicted OppA-family secreted lipoprotein. In the case of plasmid pCF10 this protein is PrgZ, which enhances the mating response to cCF10 pheromone. OppA proteins generally function with associated OppBCDF ABC transporters to import peptides. Read More

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Comparison of Microbial Communities in Colorado Potato Beetles ( Say) Collected From Different Sources in China.

Front Microbiol 2021 19;12:639913. Epub 2021 Mar 19.

Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China.

Microbial communities in insects are related to their geographical sources and contribute to adaptation to the local habitat. The Colorado potato beetle () (CPB) is a potato pest that causes serious economic losses in Xinjiang Uygur Autonomous Region (XJ) and Heilongjiang Province (HL), China. The influence of microorganisms in the invasion and dispersal of CPB is unclear. Read More

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Direct Evidence That Sunbirds' Gut Microbiota Degrades Floral Nectar's Toxic Alkaloids.

Front Microbiol 2021 11;12:639808. Epub 2021 Mar 11.

Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel.

Orange-tufted sunbirds () feed on the nectar of the tobacco tree () which contains toxic pyridine alkaloids characterized by high concentrations of anabasine and much lower concentrations of nicotine. We aimed at determining whether the gut microbiota of sunbirds harbors bacterial species that enable the birds to cope with these toxic alkaloids. An experiment that included 12 birds showed that inducing dysbiosis in sunbirds' guts by the addition of sulfamethoxazole and trimethoprim, significantly reduced the birds' ability to degrade anabasine ( = 3) compared to control birds ( = 3) with undisturbed microbiota. Read More

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Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies.

Int J Mol Sci 2021 Mar 16;22(6). Epub 2021 Mar 16.

Institute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain.

A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies-IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories (an engineered form of ) and mainly forming part of IBs and partially recovered under non-denaturing conditions. After the purification by affinity chromatography of solubilized MMP-9, four protein peaks were obtained. Read More

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Evaluation of the Estimation Capability of Response Surface Methodology and Artificial Neural Network for the Optimization of Bacteriocin-Like Inhibitory Substances Production by Gh1.

Microorganisms 2021 Mar 12;9(3). Epub 2021 Mar 12.

Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia.

Bacteriocin-like inhibitory substances (BLIS) produced by Gh1 had shown antimicrobial activity against ATCC 15313. Brain Heart Infusion (BHI) broth is used for the cultivation and enumeration of lactic acid bacteria, but there is a need to improve the current medium composition for enhancement of BLIS production, and one of the approaches is to model the optimization process and identify the most appropriate medium formulation. Response surface methodology (RSM) and artificial neural network (ANN) models were employed in this study. Read More

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The Effects of Dietary Supplementation of Strain Plasma on Skin Microbiome and Skin Conditions in Healthy Subjects-A Randomized, Double-Blind, Placebo-Controlled Trial.

Microorganisms 2021 Mar 9;9(3). Epub 2021 Mar 9.

Research and Development Strategy Department, Kirin Holdings Company, Ltd., Tokyo 164-0001, Japan.

(1) Background: strain Plasma (LC-Plasma) is a unique strain which directly activates plasmacytoid dendritic cells, resulting in the prevention against broad spectrum of viral infection. Additionally, we found that LC-Plasma intake stimulated skin immunity and prevents epicutaneous infection. The aim of this study was to investigate the effect of LC-Plasma dietary supplementation on skin microbiome, gene expression in the skin, and skin conditions in healthy subjects. Read More

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GABA-Producing Strains Isolated from Camel's Milk as Starters for the Production of GABA-Enriched Cheese.

Foods 2021 Mar 17;10(3). Epub 2021 Mar 17.

Dairy Research Institute (IPLA-CSIC), Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.

The multiple health benefits attributed to the bioactive compound γ-aminobutyric acid (GABA) have prompted the food industry to investigate the development of functional GABA-rich foods via the use of GABA-producing microorganisms. This study reports the isolation of six GABA-producing strains from camel's milk; this is the first time that such microorganisms have been isolated from milk. The sequencing and analysis of their genomes, and the characterisation of their technological and safety properties, confirmed their potential as starters. Read More

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Bio-Engineered Nisin with Increased Anti- and Selectively Reduced Anti- Activity for Treatment of Bovine Mastitis.

Int J Mol Sci 2021 Mar 27;22(7). Epub 2021 Mar 27.

APC Microbiome Ireland, T12 YT20 Cork, Ireland.

Bovine mastitis is a significant economic burden for dairy enterprises, responsible for premature culling, prophylactic and therapeutic antibiotic use, reduced milk production and the withholding (and thus wastage) of milk. There is a desire to identify novel antimicrobials that are expressly directed to veterinary applications, do not require a lengthy milk withholding period and that will not have a negative impact on the growth of lactic acid bacteria involved in downstream dairy fermentations. Nisin is the prototypical lantibiotic, a family of highly modified antimicrobial peptides that exhibit potent antimicrobial activity against many Gram-positive microbes, including human and animal pathogens including species of and . Read More

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Extract: A Non-Invasive Strategy to Obtain Adjunct Attenuated Cultures.

Foods 2021 Mar 11;10(3). Epub 2021 Mar 11.

Department of Food and Drug, University of Parma, Parco Area delle Scienze 49/A, 43124 Parma, Italy.

This study aims at proposing the use of commonly known as Spirulina, extract as a non-invasive method to attenuate the growth rate of non-starter adjunct cultures, thus preventing the over-acidification that may occur during cheese manufacturing. A preliminary screening using four different concentrations (0.20%, 0. Read More

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Dietary copper/zinc-loaded montmorillonite improved growth performance and intestinal barrier and changed gut microbiota in weaned piglets.

J Anim Physiol Anim Nutr (Berl) 2021 Apr 1. Epub 2021 Apr 1.

College of animal science, Zhejiang University, Hangzhou, China.

The effects of copper/zinc-loaded montmorillonite (Cu/Zn-Mt) on growth performance, intestinal barrier and gut microbiota of weaned pigs were investigated in the present study. A total of 108 piglets (Duroc × Landrace × Yorkshire; 6.36 kg; weaned at 21 ± 1 d age) were used in this experiment. Read More

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Subacute safety assessment of recombinant Lactococcus lactis on the gut microbiota of male Sprague-Dawley rats.

J Sci Food Agric 2021 Mar 31. Epub 2021 Mar 31.

Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, China.

Background: Lactococcus lactis strain pGSMT/MG1363 is a genetically modified microorganism (GMM) that constitutively expresses human metallothionein-I fusion protein to combine with intracellular lead. Unlike traditional probiotics, pGSMT/MG1363 lacks a history of safe use in food. Administration of microorganism could influence the gut microbial community and consequently confer health benefits or cause disadvantages to the host. Read More

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An unexpected link: The role of mammary and gut microbiota on breast cancer development and management (Review).

Oncol Rep 2021 May 31;45(5). Epub 2021 Mar 31.

Department of Basic and Clinical Sciences, University of Nicosia Medical School, CY‑1700 Nicosia, Cyprus.

Breast cancer (BC) impacts 2.3 million women each year, making it the most frequent cancer diagnosed among the female population. An unexpected link has been discovered between BC and alterations in the mammary and gut microbiota, suggesting their possible role in BC development, prevention and management. Read More

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Salivary microbial community alterations due to probiotic yogurt in preschool children with healthy deciduous teeth.

Arch Microbiol 2021 Mar 30. Epub 2021 Mar 30.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, 310006, Zhejiang, China.

Probiotics are considered valuable to human health since they improve intestinal microbial balance. Probiotics are orally taken and affect the oral microbiota, which is one of the most important parts of the human microbial community. However, there is little information on the effects of probiotics on the oral microbiota. Read More

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Metataxonomic Profiling of Native and Starter Microbiota During Ripening of Gouda Cheese Made With -Contaminated Unpasteurized Milk.

Front Microbiol 2021 12;12:642789. Epub 2021 Mar 12.

Division of Food Processing Science and Technology, Office of Food Safety, U. S. Food and Drug Administration, Bedford Park, IL, United States.

Unpasteurized milk is used to produce aged artisanal cheeses, which presents a safety concern due to possible contamination with foodborne pathogens, especially . The objective of this study was to examine the composition of the bacterial community in unpasteurized milk used to prepare Gouda cheese artificially contaminated with (~1 log CFU/ml) and assess the community dynamics and their potential interaction with during a 90-day ripening period using targeted 16S rRNA sequencing. The diversity of bacterial taxa in three batches of unpasteurized milk was not significantly different, and the microbiomes were dominated by species of , , , and . Read More

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Microbial community structure shifts and potential Symbiodinium partner bacterial groups of bleaching coral Pocillopora verrucosa in South China Sea.

Ecotoxicology 2021 Mar 27. Epub 2021 Mar 27.

CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, China.

The community structure of coral associated microorganisms will change greatly in coral bleaching. However, the relationship between specific bacteria groups and Symbiodinium, which is easy to be found in the bleaching process, has been ignored for a long time. In this study, the changes of coral microbial community during a natural bleaching event in the South China Sea were studied by 16S rRNA gene high-throughput sequencing. Read More

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Effect of peroxyacetic acid spray and storage temperature on the microbiota and sensory properties of vacuum packed subprimal cuts.

Appl Environ Microbiol 2021 Mar 26. Epub 2021 Mar 26.

Canadian Cattlemen's Association, #180, 6815-8th Street NE, Calgary, Alberta, Canada T2E 7H7.

We investigated the impact of peroxyacetic acid (PAA; 200 ppm) spray on the microbiota and shelf life of commercial vacuum packed beef stored at chiller temperatures. Ribeye cuts (n=147) were collected from a local beef plant on the day of production for two consecutive days, with one set collected at the start of work with the PAA spray nozzles turned off (control) and during the routine production with the PAA spray nozzles turned on (PAA) on each day. Packs were stored at 4, 2 and -1°C for up to 34, 104 and 180 days, and sampled at appropriate intervals for sensory assessment, microbial enumeration and microbial profiling by 16S rRNA gene amplicon analysis. Read More

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The Cheese Production Facility Microbiome Exhibits Temporal and Spatial Variability.

Front Microbiol 2021 9;12:644828. Epub 2021 Mar 9.

Department of Food Science and Technology, Oregon State University, Corvallis, OR, United States.

A primary goal of modern cheese manufacturing is consistent product quality. One aspect of product quality that remains poorly understood is the variability of microbial subpopulations due to temporal or facility changes within cheese production environments. Therefore, our aim was to quantify this variability by measuring day-day and facility-facility changes in the cheese facility microbiome. Read More

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First report on genetic characterization, cell-surface properties and pathogenicity of Lactococcus garvieae, emerging pathogen isolated from cage-cultured cobia (Rachycentron canadum).

Transbound Emerg Dis 2021 Mar 23. Epub 2021 Mar 23.

Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan.

The diseased cage-cultured cobia (Rachycentron canadum) displayed clinical signs, haemorrhagic eyes, dorsal darkness and gross pathological lesions, enlargement of spleen and liver. Haemorrhages were found in brain, heart and liver with cumulative mortality rates ranging from 20% to 50%. Extensive congestion in the heart, liver, spleen, kidney, and brain were observed histopathologically. Read More

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The number of catalytic cycles in an enzyme's lifetime and why it matters to metabolic engineering.

Proc Natl Acad Sci U S A 2021 Mar;118(13)

Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, University of Western Australia, Crawley, WA 6009, Australia.

Metabolic engineering uses enzymes as parts to build biosystems for specified tasks. Although a part's working life and failure modes are key engineering performance indicators, this is not yet so in metabolic engineering because it is not known how long enzymes remain functional in vivo or whether cumulative deterioration (wear-out), sudden random failure, or other causes drive replacement. Consequently, enzymes cannot be engineered to extend life and cut the high energy costs of replacement. Read More

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Effect of frozen storage on some characteristics of kefir samples made from cow's and goat's milk.

Food Sci Technol Int 2021 Mar 22:10820132211003710. Epub 2021 Mar 22.

Department of Food Engineering, Faculty of Engineering, Bolu Abant İzzet Baysal University, Bolu, Turkey.

This study was aimed to determine the changes in kefir samples (CK and GK) made from cow's and goat's milk during frozen storage. The CK and GK samples were first stored at +4 °C for 14 and 21 days. Thereafter, all the samples were frozen at -35 °C for 24 h and kept at -18 °C for 45 days. Read More

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High-efficiency production of 5-aminovalerate in engineered Escherichia coli controlled by an anaerobically-induced nirB promoter.

Biochem Biophys Res Commun 2021 May 19;552:170-175. Epub 2021 Mar 19.

Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, College of Food and Biological Engineering, Chengdu University, Chengdu, 610106, PR China. Electronic address:

Biobased production of 5-aminovalerate (5AVA) from biomass can support a sustainable and economic biorefinery process to produce bio-based nylon 5 for food packaging materials. Cost-competitive production of 5AVA from biomass is a key factor in the successful commercialization of nylon 5. Bioproduction of 5AVA is a promising candidate for the industrial process to the current petrochemical route. Read More

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Recombination Lactococcus lactis expressing Helicobacter pylori neutrophil-activating protein A attenuates food allergy symptoms in mice.

FEMS Microbiol Lett 2021 Mar 22. Epub 2021 Mar 22.

Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Background: Food allergy has been a significant public health issue with growing severity, prevalence and limited treatments. The neutrophil-activating protein A subunit (NapA) of Helicobacter pylori, a Th1-activating immune modulator, has been shown to have therapeutic potential in allergic diseases.

Objective: We adopted Lactococcus lactis (L. Read More

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Study of microbiome changes in patients with ulcerative colitis in the Central European part of Russia.

Heliyon 2021 Mar 10;7(3):e06432. Epub 2021 Mar 10.

Department of Genetics, Cytology and Bioengineering, Voronezh State University, 394018 Voronezh, Russia.

Ulcerative colitis (UC) is an inflammatory disease that affects the colon and rectum. Recently, evidence has emerged about the influence of microbiota on the development of this disease. However, studies on the role of intestinal microbiota in the pathogenesis of UC have been incomplete. Read More

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Gene-Trait Matching and Prevalence of Nisin Tolerance Systems in .

Front Bioeng Biotechnol 2021 3;9:622835. Epub 2021 Mar 3.

R&D, Chr. Hansen A/S, Hørsholm, Denmark.

cheese starter cultures typically contain a mix of many strains and may include variants that produce and/or tolerate the antimicrobial bacteriocin nisin. Nisin is well-established as an effective agent against several undesirable Gram-positive bacteria in cheese and various other foods. In the current study, we have examined the effect of nisin on 710 individual strains during milk fermentations. Read More

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Comparative profiling of lactic acid bacteria isolates in optimized and spontaneous fermentation of cowpea leaves.

Food Sci Nutr 2021 Mar 23;9(3):1651-1664. Epub 2021 Jan 23.

Department of Food Science, Nutrition and Technology University of Nairobi Nairobi Kenya.

In as much as spontaneous fermentation of cowpea leaves enhances product diversification, the process is rather slow with poor product quality. Limited work has been undertaken to provide input toward standardization of the process and enhancing of product quality. The current study sought to evaluate the in-process fermentative bacteria profile changes due to the effect of optimization of fermentation process of cowpea leaves. Read More

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