226,555 results match your criteria labeled rhsod


Nanoscale Dynamics of Cellulose Digestion by the Cellobiohydrolase TrCel7A.

J Biol Chem 2021 Jul 30:101029. Epub 2021 Jul 30.

Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802. Electronic address:

Understanding the mechanism by which cellulases from bacteria, fungi, and protozoans catalyze the digestion of lignocellulose is important for developing cost effective strategies for bioethanol production. Cel7A from the fungus Trichoderma reesei is a model exoglucanase that degrades cellulose strands from their reducing ends by processively cleaving individual cellobiose units. Despite being one of the most studied cellulases, the binding and hydrolysis mechanisms of Cel7A are still debated. Read More

View Article and Full-Text PDF

Effect of Various Laser-Activated Irrigation Protocols on Endodontic Sealer Penetration into the Dentinal Tubules: A Confocal Laser Scanning Microscopy Study.

Photobiomodul Photomed Laser Surg 2021 Jul 30. Epub 2021 Jul 30.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, P.R. China.

To study the effect of various laser-activated irrigation protocols on endodontic sealer penetration into the dentinal tubules. Laser-activated irrigation is helpful for smear layer removal and thus might increase the penetration of endodontic sealer into the dentinal tubules. Forty-eight mandibular premolars with straight canals were instrumented and divided into four groups: C-I group, conventional needle irrigation with sodium hypochlorite (NaOCl) followed by ethylenediaminetetraacetic acid (EDTA); Er:YAG-I, Er,Cr:YSGG-I, and Nd:YAP-I groups, NaOCl and EDTA irrigations activated by erbium:yttrium-aluminum-garnet (Er:YAG), erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG), and neodymium:yttrium-aluminum-perovskite (Nd:YAP) lasers, respectively. Read More

View Article and Full-Text PDF

All-Optical Wide-Field Selective Imaging of Fluorescent Nanodiamonds in Cells, and .

ACS Nano 2021 Aug 2. Epub 2021 Aug 2.

Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Inage-ku, Chiba 263-8555, Japan.

Fluorescence imaging is a critical tool to understand the spatial distribution of biomacromolecules in cells and , providing information on molecular dynamics and interactions. Numerous valuable insights into biological systems have been provided by the specific detection of various molecular species. However, molecule-selective detection is often hampered by background fluorescence, such as cell autofluorescence and fluorescence leakage from molecules stained by other dyes. Read More

View Article and Full-Text PDF

Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.

Biochemistry 2021 Aug 2. Epub 2021 Aug 2.

Department of Chemical Biology, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany.

The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag, and CLIP-tag allow the covalent labeling of fusion proteins with synthetic molecules for applications in bioimaging and biotechnology. To guide the selection of an SLP-substrate pair and provide guidelines for the design of substrates, we report a systematic and comparative study of the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag, and CLIP-tag. HaloTag7 reaches almost diffusion-limited labeling rate constants with certain rhodamine substrates, which are more than 2 orders of magnitude higher than those of SNAP-tag for the corresponding substrates. Read More

View Article and Full-Text PDF

Micro-computed tomography analysis of root canal morphology and thickness of crown and root of mandibular incisors in Chinese population.

Clin Oral Investig 2021 Aug 2. Epub 2021 Aug 2.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, 14#, 3rd Section of RenMin South Road, Chengdu, 610041, China.

Objectives: The aim of this study was to investigate the root canal morphology and the thickness of crown and root of mandibular incisors in a Chinese subpopulation by micro-computed tomography (micro-CT).

Methods: In total, 208 mandibular incisors were scanned using micro-CT. The anatomical features of the canals (canal configuration, apical constriction, foramen-to-apex distance, accessory canal vertical distribution, and canal geometrical parameters) and the thickness of the crown and root 2/3 were evaluated. Read More

View Article and Full-Text PDF

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging.

J Vis Exp 2021 Jul 16(173). Epub 2021 Jul 16.

Molloy College.

Mesenchymal stem cells (MSC) have traditionally been studied for their regenerative properties, but more recently, their immunoregulatory characteristics have been at the forefront. They interact with and regulate immune cell activity. The focus of this study is the MSC regulation of macrophage phagocytic activity. Read More

View Article and Full-Text PDF

Facile surface functional polyetheretherketone with antibacterial and immunoregulatory activities for enhanced regeneration toward bacterium-infected bone destruction.

Drug Deliv 2021 Dec;28(1):1649-1663

Department of Orthopedic Surgery, Fujian Medical University Union Hospital, Fuzhou, China.

Existing biologically inert or unmodified implants to treat infectious bone defects or osteomyelitis still cannot effectively solve bacterial infection and osseointegration. In this work, a simple co-deposition strategy was developed to modify porous polyetheretherketone (PEEK) with improved antibacterial activity and controllable immunoregulatory ability. After PEEK was treated by HSO to obtain porous PEEK (SPEEK), the self-polymerization of dopamine was operated on SPEEK in the solution of dopamine and gentamicin sulfate (GS) to prepare polydopamine (pDA) and GS layer-modified SPEEK (labeled as SPEEK-pDA-GS). Read More

View Article and Full-Text PDF
December 2021

Development of an Easily Bioconjugatable Water-Soluble Single-Photon Emission-Computed Tomography/Optical Imaging Bimodal Imaging Probe Based on the aza-BODIPY Fluorophore.

J Med Chem 2021 Aug 2. Epub 2021 Aug 2.

Institut de Chimie Moléculaire de l'Université de Bourgogne, ICMUB UMR CNRS 6302, Université Bourgogne Franche-Comté, Dijon 21000, France.

A water-soluble fluorescent aza-BODIPY platform (Wazaby) was prepared and functionalized by a polyazamacrocycle agent and a bioconjugable arm. The resulting fluorescent derivative was characterized and bioconjugated onto a trastuzumab monoclonal antibody as a vector. After bioconjugation, the imaging agent appeared to be stable in serum (>72 h at 37 °C) and specifically labeled HER-2-positive breast tumors slices. Read More

View Article and Full-Text PDF

A review of machine learning for cardiology.

Minerva Cardiol Angiol 2021 Aug 2. Epub 2021 Aug 2.

Computer Science Department, University of Turin, Turin, Italy.

This paper reviews recent cardiology literature and reports how Artificial Intelligence Tools (specifically, Machine Learning techniques) are being used by physicians in the field. Each technique is introduced with enough details to allow the understanding of how it works and its intent, but without delving into details that do not add immediate benefits and require expertise in the field. We specifically focus on the principal Machine Learning based risk scores used in cardiovascular research. Read More

View Article and Full-Text PDF

Tonotopic distribution and inferior colliculus projection pattern of inhibitory and excitatory cell types in the lateral superior olive of Mongolian gerbils.

J Comp Neurol 2021 Aug 2. Epub 2021 Aug 2.

Department of Anatomy and Neurobiology, Hearing Research Group, Northeast Ohio Medical University, Hearing Research Group, Rootstown, OH, United States.

Sound localization critically relies on brainstem neurons that compare information from the two ears. The conventional role of the lateral superior olive (LSO) is extraction of intensity differences; however, it is increasingly clear that relative timing, especially of transients, is also an important function. Cellular diversity within the LSO that is not well understood may underlie its multiple roles. Read More

View Article and Full-Text PDF

Fluorescence resonance energy transfer-based screening for protein kinase C ligands using 6-methoxynaphthalene-labeled 1,2-diacylglycerol-lactones.

Org Biomol Chem 2021 Aug 2. Epub 2021 Aug 2.

Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan.

Protein kinase C (PKC) is associated with a central cellular signal transduction pathway and disorders such as cancer and Alzheimer-type dementia and is therefore a target for the treatment of these diseases. The development of simple methods suitable for high-throughput screening to find potent PKC ligands is desirable. We have developed an assay based on fluorescence-quenching screening with a solvatochromic fluorophore attached to a competitive probe and its alternative method based on Förster/fluorescence resonance energy transfer (FRET) phenomena. Read More

View Article and Full-Text PDF

Vexing visits: Variables correlating with physician perceptions of patients as "difficult" and overall enjoyment of their delivery of primary ambulatory care.

Int J Psychiatry Med 2021 Aug 1:912174211032037. Epub 2021 Aug 1.

Department of Graduate Medical Education, 23071AdventHealth East Orlando, AdventHealth East Orlando, Orlando, FL, USA.

All physicians experience some patients described as "difficult." Their prevalence negatively impacts work satisfaction. Prior research identified factors present when physicians perceive patients as difficult. Read More

View Article and Full-Text PDF

Comparison of beta-ligands used in cattle production: structures, safety, and biological effects.

J Anim Sci 2021 Aug;99(8)

RLEConsult, Bolivia, NC, USA.

Technologies that increase the efficiency and sustainability of food animal production to provide meat for a growing population are necessary and must be used in a manner consistent with good veterinary practices, approved labeled use, and environmental stewardship. Compounds that bind to beta-adrenergic receptors (β-AR), termed beta-adrenergic receptor ligands (β-ligands), are one such technology and have been in use globally for many years. Though all β-ligands share some similarities in structure and function, the significance of their structural and pharmacological differences is sometimes overlooked. Read More

View Article and Full-Text PDF

Flower-like gold nanoparticles labeled and silver deposition rapid vertical flow technology for highly sensitive detection of antibodies.

Analyst 2021 Aug 2. Epub 2021 Aug 2.

College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China.

To prevent the transmission of brucellosis, rapid vertical flow technology (RVFT) was developed to detect brucellosis antibodies. To improve the sensitivity of the technique, lipopolysaccharides (LPS) were purified and used to detect brucellosis antibodies. To improve the sensitivity of serum antibody detection, a single multifunctional buffer was established in whole blood and other biological samples, and the advantages of the lateral flow immunoassay were retained. Read More

View Article and Full-Text PDF

External Validation of Two Nomograms Developed for Ga-PSMA-11 Applied to the Prostate-specific Membrane Antigen Tracer F-DCFPyl: Is Prediction of the Optimal Timing of Salvage Therapy Feasible?

Eur Urol Open Sci 2021 Jun 29;28:47-51. Epub 2021 Apr 29.

Department of Urology, The Netherlands Cancer Institute, Prostate Cancer Network Netherlands, Amsterdam, The Netherlands.

Two nomograms have been developed to predict the outcome of positron emission tomography (PET)/computed tomography (CT) imaging withGa-labeled ligands for prostate-specific membrane antigen (Ga-PSMA) for patients with rising prostate-specific antigen after radical prostatectomy (RP). These nomograms quantify the ability of PSMA PET/CT to detect prostate cancer recurrences, and therefore provide critical information in determining the optimal timing for PSMA PET/CT in guiding salvage therapies. We validated the ability of these nomograms to accurately predict PET/CT outcome using another ligand tracer, F-DCFPyL. Read More

View Article and Full-Text PDF

Towards data-driven stroke rehabilitation via wearable sensors and deep learning.

Proc Mach Learn Res 2020 Aug;126:143-171

Center for Data Science, Courant Institute of Mathematical Sciences, New York University.

Recovery after stroke is often incomplete, but rehabilitation training may potentiate recovery by engaging endogenous neuroplasticity. In preclinical models of stroke, high doses of rehabilitation training are required to restore functional movement to the affected limbs of animals. In humans, however, the necessary dose of training to potentiate recovery is not known. Read More

View Article and Full-Text PDF

An Integrated Deep Network for Cancer Survival Prediction Using Omics Data.

Front Big Data 2021 16;4:568352. Epub 2021 Jul 16.

Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, United States.

As a highly sophisticated disease that humanity faces, cancer is known to be associated with dysregulation of cellular mechanisms in different levels, which demands novel paradigms to capture informative features from different omics modalities in an integrated way. Successful stratification of patients with respect to their molecular profiles is a key step in precision medicine and in tailoring personalized treatment for critically ill patients. In this article, we use an integrated deep belief network to differentiate high-risk cancer patients from the low-risk ones in terms of the overall survival. Read More

View Article and Full-Text PDF

ExoHCR: a sensitive assay to profile PD-L1 level on tumor exosomes for immunotherapeutic prognosis.

Biophys Rep 2020 Dec 23;6(6):290-298. Epub 2020 Nov 23.

Center for Pharmaceutical Engineering and Sciences, Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298, USA.

Cancer immunotherapy has made recent breakthrough, including immune checkpoint blockade (ICB) that inhibits immunosuppressive checkpoints such as programmed cell death protein 1 (PD-1) and programmed death-ligand 1 (PD-L1). However, most cancer patients do not durably respond to ICB. To predict ICB responses for patient stratification, conventional immunostaining has been used to analyze the PD-L1 expression level on biopsied tumor tissues but has limitations of invasiveness and tumor heterogeneity. Read More

View Article and Full-Text PDF
December 2020

A sensitive homogeneous enzyme assay for euchromatic histone-lysine-N-methyltransferase 2 (G9a) based on terbium-to-quantum dot time-resolved FRET.

Bioimpacts 2021 8;11(3):173-179. Epub 2020 Jul 8.

NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, Orsay, France.

Histone modifying enzymes include several classes of enzymes that are responsible for various post-translational modifications of histones such as methylation and acetylation. They are important epigenetic factors, which may involve several diseases and so their assay, as well as screening of their inhibitors, are of great importance. Herein, a bioassay based on terbium-to-quantum dot (Tb-to-QD) time-resolved Förster resonance energy transfer (TR-FRET) was developed for monitoring the activity of G9a, the euchromatic histone-lysine N-methyltransferase 2. Read More

View Article and Full-Text PDF

A Survey of Unsupervised Deep Domain Adaptation.

ACM Trans Intell Syst Technol 2020 Sep 5;11(5):1-46. Epub 2020 Jul 5.

Washington State University, USA.

Deep learning has produced state-of-the-art results for a variety of tasks. While such approaches for supervised learning have performed well, they assume that training and testing data are drawn from the same distribution, which may not always be the case. As a complement to this challenge, single-source unsupervised domain adaptation can handle situations where a network is trained on labeled data from a source domain and unlabeled data from a related but different target domain with the goal of performing well at test-time on the target domain. Read More

View Article and Full-Text PDF
September 2020

Prevalence of Strongyle Infection and Associated Risk Factors in Horses and Donkeys in and around Mekelle City, Northern Part of Ethiopia.

Vet Med Int 2021 21;2021:9430824. Epub 2021 Jul 21.

College of Veterinary Medicine, Samara University, P.O. Box 132, Samara, Ethiopia.

Background: In Ethiopia, equines serve in traction power, carting, recreation, festival packing, riding, transportation, and other activities since time immemorial. Strongyles are common equine health problems in Ethiopia though research based data on equine strongyles are limited particularly in the study areas, in and around Mekelle city. Therefore, the present study was intended to estimate the prevalence of common equine strongyles in and around Mekelle city from November 2018 to April 2019 and to assess risk factors associated with infection of strongyle parasites as well. Read More

View Article and Full-Text PDF

Prevalence of the SNP rs10774671 of the OAS1 gene in Mexico as a possible predisposing factor for RNA virus disease.

Int J Mol Epidemiol Genet 2021 15;12(3):52-60. Epub 2021 Jun 15.

Universidad Autónoma de Nuevo León, Centro de Investigación y Desarrollo en Ciencias de la Salud Monterrey, Nuevo León, México.

The COVID-19 pandemic has revealed the susceptibility of certain populations to RNA virus infection. This variety of agents is currently the cause of severe respiratory diseases (SARS-CoV2 and Influenza), Hepatitis C, measles and of high prevalence tropical diseases that are detected throughout the year (Dengue and Zika). The rs10774671 polymorphism is a base change from G to A in the last nucleotide of intron-5 of the OAS1 gene. Read More

View Article and Full-Text PDF

Recovery of surfaces and functions in high dimensions: sampling theory and links to neural networks.

SIAM J Imaging Sci 2021 10;14(2):580-619. Epub 2021 May 10.

Department of Electrical and Computer Engineering, University of Iowa, Iowa City, IA 52242.

Several imaging algorithms including patch-based image denoising, image time series recovery, and convolutional neural networks can be thought of as methods that exploit the manifold structure of signals. While the empirical performance of these algorithms is impressive, the understanding of recovery of the signals and functions that live on manifold is less understood. In this paper, we focus on the recovery of signals that live on a union of surfaces. Read More

View Article and Full-Text PDF

Low-dose total body irradiation facilitates antitumoral Th1 immune responses.

Theranostics 2021 16;11(16):7700-7714. Epub 2021 Jun 16.

Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tuebingen, Germany.

CD4 T helper cells are capable of mediating long-term antitumoral immune responses. We developed a combined immunotherapy (COMBO) using tumor antigen-specific T helper 1 cells (Tag-Th1), dual PD-L1/LAG-3 immune checkpoint blockade, and a low-dose total body irradiation (TBI) of 2 Gy, that was highly efficient in controlling the tumor burden of non-immunogenic RIP1-Tag2 mice with late-stage endogenous pancreatic islet carcinomas. In this study, we aimed to explore the impact of 2 Gy TBI on the treatment efficacy and the underlying mechanisms to boost CD4 T cell-based immunotherapies. Read More

View Article and Full-Text PDF

Combination of Xuesaitong and Aspirin Based on the Antiplatelet Effect and Gastrointestinal Injury: Study Protocol for a Randomized Controlled Noninferiority Trial.

Evid Based Complement Alternat Med 2021 13;2021:5552506. Epub 2021 Jul 13.

Department of Pharmacy, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Background: Aspirin is the first-line medication for prevention and treatment of coronary heart disease (CHD). However, long-term use of aspirin resulting in gastrointestinal mucosal injury and bleeding limits the regularity of medication. Xuesaitong is a marketed Chinese medicine contained main active component in Panax notoginseng saponins (PNS), which can significantly inhibit platelet aggregation in patients with CHD. Read More

View Article and Full-Text PDF

Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons.

Front Physiol 2021 15;12:644929. Epub 2021 Jul 15.

Department of Anatomy and Physiology, University of Melbourne, Melbourne, VIC, Australia.

Piezo2 is a mechanically gated ion-channel that has a well-defined role in innocuous mechanical sensitivity, but recently has also been suggested to play a role in mechanically induced pain. Here we have explored a role for Piezo2 in mechanically evoked bone nociception in Sprague Dawley rats. We have used an electrophysiological bone-nerve preparation to record the activity of single Aδ bone afferent neurons in response to noxious mechanical stimulation, after Piezo2 knockdown in the dorsal root ganglia with intrathecal injections of Piezo2 antisense oligodeoxynucleotides, or in control animals that received mismatch oligodeoxynucleotides. Read More

View Article and Full-Text PDF

Multiple Sources of Cholinergic Input to the Superior Olivary Complex.

Front Neural Circuits 2021 15;15:715369. Epub 2021 Jul 15.

Department of Anatomy and Neurobiology, Hearing Research Focus Group, Northeast Ohio Medical University, Rootstown, OH, United States.

The superior olivary complex (SOC) is a major computation center in the brainstem auditory system. Despite previous reports of high expression levels of cholinergic receptors in the SOC, few studies have addressed the functional role of acetylcholine in the region. The source of the cholinergic innervation is unknown for all but one of the nuclei of the SOC, limiting our understanding of cholinergic modulation. Read More

View Article and Full-Text PDF

Physical Activity and Total Daily Energy Expenditure in Older US Adults: Constrained versus Additive Models.

Med Sci Sports Exerc 2021 Jul 30. Epub 2021 Jul 30.

Center for Health Promotion Disease Prevention, University of North Carolina-Chapel Hill, Chapel Hill, NC Department of Nutrition, Gillings School of Global Public Health, University of North Carolina-Chapel Hill, Chapel Hill, NC Division of Endocrinology, Metabolism, and Diabetes, University of Colorado Anschutz Medical Campus, Aurora, CO Anschutz Health and Wellness Center, University of Colorado Anschutz Medical Campus, Aurora, CO Division of Epidemiology and Genetics, Metabolic Epidemiology Branch, National Cancer Institute, Bethesda, MD Department of Kinesiology and Public Health, California Polytechnic State University, San Luis Obispo, CA Department of Evolutionary Anthropology, Duke University, Durham, NC Duke Global Health Institute, Duke University, Durham, NC Nutritional Sciences, University of Wisconsin-Madison, Madison, WI Risk Factor Assessment Branch, Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, MD.

Purpose: To examine the shape of the relationship between physical activity (PA) and total energy expenditure (TEE) and to explore the role of energy balance status (negative, stable, positive) in influencing this association.

Methods: Cross-sectional. Participants were 584 older adults (50-74 yrs. Read More

View Article and Full-Text PDF

Determination of Guanfacine in Oral Fluid and Serum of Children and Adolescents With Attention-Deficit/Hyperactivity Disorder: A Short Communication.

Ther Drug Monit 2021 Jul 30. Epub 2021 Jul 30.

Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany Institute for Pharmacy and Food Chemistry, University of Würzburg, Würzburg, Germany.

Background: Guanfacine, a selective α2A-adrenoreceptor agonist, is a second-line medication for treating children and adolescents with attention-deficit/hyperkinetic disorder (ADHD). The dosage administered as milligram per body weight to balance the potential benefits and risks of treatment. Therapeutic drug monitoring (TDM) is useful for identifying a patient's therapeutic window to optimize individual drug dosing and reduce the risk of adverse drug reactions. Read More

View Article and Full-Text PDF

Diagnostic Performance of Automated MRI Volumetry by icobrain dm for Alzheimer's Disease in a Clinical Setting: A REMEMBER Study.

J Alzheimers Dis 2021 Jul 27. Epub 2021 Jul 27.

Reference Center for Biological Markers of Dementia (BIODEM), Laboratory of Neurochemistry and Behavior, University of Antwerp, Antwerp, Belgium.

Background: Magnetic resonance imaging (MRI) has become important in the diagnostic work-up of neurodegenerative diseases. icobrain dm, a CE-labeled and FDA-cleared automated brain volumetry software, has shown potential in differentiating cognitively healthy controls (HC) from Alzheimer's disease (AD) dementia (ADD) patients in selected research cohorts.

Objective: This study examines the diagnostic value of icobrain dm for AD in routine clinical practice, including a comparison to the widely used FreeSurfer software, and investigates if combined brain volumes contribute to establish an AD diagnosis. Read More

View Article and Full-Text PDF