4,936 results match your criteria lab array


Faster super-resolution ultrasound imaging with a deep learning model for tissue decluttering and contrast agent localization.

Biomed Phys Eng Express 2021 Oct 13. Epub 2021 Oct 13.

Bioengineering, The University of Texas at Dallas, 800 W. Campbell, Richardson, Texas, 75080-3021, UNITED STATES.

Super-resolution ultrasound (SR-US) imaging allows visualization of microvascular structures as small as tens of micrometers in diameter. However, use in the clinical setting has been impeded in part by ultrasound (US) acquisition times exceeding a breath-hold and by the need for extensive offline computation. Deep learning techniques have been shown to be effective in modeling the two more computationally intensive steps of microbubble (MB) contrast agent detection and localization. Read More

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October 2021

Striatal topographical organization: Bridging the gap between molecules, connectivity and behavior.

Eur J Histochem 2021 Oct 13;65(s1). Epub 2021 Oct 13.

Brain Mapping Lab, Department of Biomedical, Dental Sciences and Morphological and Functional Images, University of Messina.

The striatum represents the major hub of the basal ganglia, receiving projections from the entire cerebral cortex and it is assumed to play a key role in a wide array of complex behavioral tasks. Despite being extensively investigated during the last decades, the topographical organization of the striatum is not well understood yet. Ongoing efforts in neuroscience are focused on analyzing striatal anatomy at different spatial scales, to understand how structure relates to function and how derangements of this organization are involved in various neuropsychiatric diseases. Read More

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October 2021

A high temporal/spatial resolution neuro-architecture study of rodent brain by wideband echo planar imaging.

Sci Rep 2021 Oct 12;11(1):20243. Epub 2021 Oct 12.

Graduate Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei, Taiwan.

Latest simultaneous multi-slice (SMS) methods greatly benefit MR efficiency for recent studies using parallel imaging technique. However, these methods are limited by the requirement of array coils. The proposed Coherent Wideband method, which employs an extended field of view to separate multiple excited slices, can be applied to any existing MRI instrument, even those without array coils. Read More

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October 2021

High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

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September 2021

A photofabricated honeycomb micropillar array for loss-free trapping of microfluidic droplets and application to digital PCR.

Lab Chip 2021 Oct 12;21(20):3933-3941. Epub 2021 Oct 12.

Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310023, China.

Droplet microfluidics is a promising platform for various biological and biomedical applications. Among which, droplet-based digital PCR (ddPCR) is one of the most challenging examples, with practical issues involving possible fusion/fission of droplets during PCR thermocycling and difficulties of indexing them for real-time monitoring. While spatially trapped droplet arrays may be helpful, they currently are either of low trapping density or suffer from high droplet loss. Read More

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October 2021

A Bioinspired Adhesive-Integrated-Agent Strategy for Constructing Robust Gas-Sensing Arrays.

Adv Mater 2021 Oct 10:e2106067. Epub 2021 Oct 10.

CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, CAS Center for Excellence in Nanoscience, Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CAS-HKU Joint Laboratory on New Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Gas sensors based on organic molecules are attractive for their tailored molecular structures and controllable functions, but weak interfacial adhesion between sensing materials and supporting substrates has severely hampered their practical applications, particularly in harsh environments. Here, inspired by the combined anchoring-recognizing feature of natural olfactory systems, an adhesive-integrated-agent strategy to integrate the adhesive unit (poly(dimethylsiloxane)) with the sensing unit (organoplatinum(II)) into one chemistry entity, creating robust and sensitive nanobelt array gas sensors is demonstrated. Systematic theoretical and experimental studies reveal that incorporating adhesive units significantly enhances the interfacial adhesion of the array sensors and gas-bridged super-exchange electronic couplings of sensing units ensure their efficient gas-sensing performance. Read More

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October 2021

Vertically Stacked Full Color Quantum Dots Phototransistor Arrays for High-Resolution and Enhanced Color-Selective Imaging.

Adv Mater 2021 Oct 11:e2106215. Epub 2021 Oct 11.

Displays and Devices Research Lab. School of Electrical and Electronics Engineering, Chung-Ang University, Seoul, 06974, Republic of Korea.

Color-selective multi-functional and multiplexed photodetectors have attracted considerable interest with the increasing demand for color filter free optoelectronics which can simultaneously process multispectral signal via minimized system complexity. The low efficiency of color-filter technology and conventional laterally pixelated photodetector array structures often limit opportunities for widespread realization of high-density photodetectors. Here, we develop low-temperature solution-processed vertically stacked full color quantum dot (QD) phototransistor arrays on plastic substrates for high-resolution color-selective photosensor applications. Read More

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October 2021

The value of human data annotation for machine learning based anomaly detection in environmental systems.

Water Res 2021 Sep 27;206:117695. Epub 2021 Sep 27.

Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland; Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

Anomaly detection is the process of identifying unexpected data samples in datasets. Automated anomaly detection is either performed using supervised machine learning models, which require a labelled dataset for their calibration, or unsupervised models, which do not require labels. While academic research has produced a vast array of tools and machine learning models for automated anomaly detection, the research community focused on environmental systems still lacks a comparative analysis that is simultaneously comprehensive, objective, and systematic. Read More

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September 2021

Isolation of Human Neutrophils from Whole Blood and Buffy Coats.

J Vis Exp 2021 09 17(175). Epub 2021 Sep 17.

Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children's Hospital Boston, and Dana-Farber/Harvard Cancer Center; Department of Microbiology, Faculty of Science, Mahidol University;

Neutrophils (PMNs) are the most abundant leukocytes in human circulation, ranging from 40 to 70% of total blood leukocytes. They are the first cells recruited at the site of inflammation via rapid extravasation through vessels. There, neutrophils perform an array of functions to kill invading pathogens and mediate immune signaling. Read More

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September 2021

Administration of pilocarpine by microneedle patch as a novel method for cystic fibrosis sweat testing.

Bioeng Transl Med 2021 Sep 3;6(3):e10222. Epub 2021 Apr 3.

School of Chemical and Biomolecular Engineering Georgia Institute of Technology Atlanta Georgia USA.

The sweat test is the gold standard for the diagnosis of cystic fibrosis (CF). The test utilizes iontophoresis to administer pilocarpine to the skin to induce sweating for measurement of chloride concentration in sweat. However, the sweat test procedure needs to be conducted in an accredited lab with dedicated instrumentation, and it can lead to inadequate sweat samples being collected in newborn babies and young children due to variable sweat production with pilocarpine iontophoresis. Read More

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September 2021

Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer.

STAR Protoc 2021 Dec 17;2(4):100841. Epub 2021 Sep 17.

Cancer Epigenetic Biology and Therapeutics Lab, Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia.

Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell "omic platforms," both in an unbiased manner or after selection of a specific cell population. For complete details on the use and execution of this protocol, please refer to Valdes-Mora et al. Read More

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December 2021

Phase transitions in Virology.

Rep Prog Phys 2021 Sep 29. Epub 2021 Sep 29.

Institute for Integrative Systems Biology, Catedrático Agustín Escardino, Valencia, 46980, SPAIN.

Viruses have stablished relationships with almost every other living organism on Earth and at all levels of biological organization: from other viruses up to entire ecosystems. In most cases, peacefully coexisting with their hosts, but in most relevant cases, parasitizing them and inducing diseases and pandemics, such as the AIDS and the most recent avian influenza and COVID-19 pandemic events, with a huge impact on health, society, and economy. Viruses are playing an essential role in shaping the eco-evolutionary dynamics of their hosts, and have been also involved in some of the major evolutionary innovations either by working as vectors of genetic information or by being themselves coopted by the host into their genomes. Read More

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September 2021

A bioinspired, passive microfluidic lobe filtration system.

Lab Chip 2021 09 28;21(19):3762-3774. Epub 2021 Sep 28.

Department of Chemical & Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA.

Size-based microfluidic filtration systems can be affected by clogging, which prevents their use in high-throughput and continuous applications. To address these concerns, we have developed two microfluidic lobe filters bioinspired by the filtration mechanism of two species of manta ray. These chips enable filtration of particles around 10-30 μm with precise control and high throughput by using two arrays of equally spaced filter lobes. Read More

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September 2021

Real-World Outcomes Using a Spinal Cord Stimulation Device Capable of Combination Therapy for Chronic Pain: A European, Multicenter Experience.

J Clin Med 2021 Sep 10;10(18). Epub 2021 Sep 10.

Boston Scientific Neuromodulation, Valencia, CA 91355, USA.

Given the differing mechanisms thought to underlie therapeutic sub- and supra-perception-based neurostimulative modalities, Spinal Cord Stimulation (SCS) systems designed for combined delivery of these approaches may help improve analgesic outcomes and quality of life, and reduce treatment failures. This multicenter, observational case-series evaluated 188 patients with chronic back and/or leg pain implanted with an SCS device capable of sequential or simultaneous delivery of sub-perception and supra-perception stimulation programming (i.e. Read More

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September 2021

Fusing MEMS technology with lab-on-chip: nanoliter-scale silicon microcavity arrays for digital DNA quantification and multiplex testing.

Microsyst Nanoeng 2020 5;6:82. Epub 2020 Oct 5.

Robert Bosch GmbH, Corporate Sector Research, Microsystems and Nanotechnologies, Robert-Bosch-Campus 1, 71272 Renningen, Germany.

We report on the development of a microfluidic multiplexing technology for highly parallelized sample analysis via quantitative polymerase chain reaction (PCR) in an array of 96 nanoliter-scale microcavities made from silicon. This PCR array technology features fully automatable aliquoting microfluidics, a robust sample compartmentalization up to temperatures of 95 °C, and an application-specific prestorage of reagents within the 25 nl microcavities. The here presented hybrid silicon-polymer microfluidic chip allows both a rapid thermal cycling of the liquid compartments and a real-time fluorescence read-out for a tracking of the individual amplification reactions taking place inside the microcavities. Read More

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October 2020

High-resolution imaging and fast number estimation of suspended particles using dewetted polymer microlenses in a microfluidic channel.

Micron 2021 Sep 16;151:103148. Epub 2021 Sep 16.

Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi, UP, 221005, India. Electronic address:

We have fabricated polymer micro-lens array by self-organized dewetting inside the microchannel, which shows remarkable enhancement in the resolution, contrast and more than 10 times add-on magnification to a microscope. These lenses are demonstrated to resolve sub-micrometer features and detect moving micro-particles when suspension is flown in a microchannel. Polystyrene (PS) micro-lenses are fabricated on a polydimethylsiloxane (PDMS) substrate using the controlled dewetting of PS thin film then this PDMS substrate is used to close the microchannel with inverted micro-lenses on it. Read More

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September 2021

Detection of transdermal biomarkers using gradient-based colorimetric array sensor.

Biosens Bioelectron 2021 Sep 20;195:113650. Epub 2021 Sep 20.

Center for Bioelectronics and Biosensors, The Biodesign Institute, Arizona State University, USA; Department of Electrical Engineering and Computer Science, South Dakota State University, Brookings, SD, 57007, USA. Electronic address:

Accurate assessment of dietary macronutrients intake is critical for the effective management of multiple diseases, such as obesity, diabetes, cardiovascular disease, metabolic disease, and cancer. Conventional self-reporting method is burdensome, inaccurate, and often biased. Though blood analysis and breath analysis can provide evidence-based information, they are either invasive or subject to human errors. Read More

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September 2021

Human neuromuscular junction on micro-structured microfluidic devices implemented with a custom micro electrode array (MEA).

Lab Chip 2021 Sep 24. Epub 2021 Sep 24.

IGMM, University of Montpellier, CNRS, Montpellier, France.

In the neuromuscular system, signal transmission from motor neurons (MNs) to innervated muscle fibers is crucial for their synaptic function, viability, and maintenance. In order to better understand human neuromuscular junction (hNMJ) functionality, it is important to develop on-a-chip devices with human cells. To investigate this cell network, microfluidic platforms are useful to grow different cell types in isolated compartments. Read More

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September 2021

Functional Characterization of Type III-A CRISPR-Cas in a Clinical Human Methicillin-R Strain.

CRISPR J 2021 Oct 23;4(5):686-698. Epub 2021 Sep 23.

Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, P.R. China; University of Copenhagen, Copenhagen, Denmark.

CRISPR with its genes is an adaptive immune system that protects prokaryotes against foreign genetic elements. The type III-A CRISPR-Cas system is rarely found in , and little is known about its function in . Here, we describe the genome characteristics of the clinical methicillin-resistant (MRSA) strain TZ0912, carrying a type III-A CRISPR-Cas system. Read More

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October 2021

CXCL8, CXCL9, and CXCL10 serum levels increase in syphilitic patients with seroresistance.

J Clin Lab Anal 2021 Sep 23:e24016. Epub 2021 Sep 23.

Center of Clinical Laboratory Medicine, The Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Zhejiang, China.

Background: Recently, the rise of syphilitic seroresistance brings great confusion to the clinical diagnosis and treatment of syphilis, and no clear diagnostic marker has been found to distinguish syphilitic seroresistance from other progression of syphilis. This study evaluated the serum chemokines levels of CCL2, CXCL8, CXCL9, and CXCL10 and its correlation with blood routine, coagulation, and biochemical indexes in seroresistant syphilitic patients.

Method: Serum levels of chemokines were quantitatively determined by Flow Cytometric Bead Array (CBA). Read More

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September 2021

Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.

Plant Cell Rep 2021 Sep 23. Epub 2021 Sep 23.

Key Laboratory of Horticultural Plant Biology Ministry of Education, Huazhong Agricultural University, Wuhan, People's Republic of China.

Key Message: Here, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Read More

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September 2021

Low-temperature grown vertically aligned carbon nanotube array for an optimal infrared bolometer.

Nanotechnology 2021 Oct 11;32(50). Epub 2021 Oct 11.

Center for Nanostructures, Santa Clara University, Santa Clara, CA, United States of America.

Vertically aligned carbon nanotube (VACNT) arrays have been explored as an absorber of thermal-type photodetectors. A long and dense VACNT array absorbs a wide spectral range of incident light with high absorption rate, but has a high thermal mass that results in a low response speed. To achieve a small thermal mass, a shorter and less dense VACNT array is needed. Read More

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October 2021

Robust three-dimensional nanotube-in-micropillar array electrodes to facilitate size independent electroporation in blood cell therapy.

Lab Chip 2021 Sep 21. Epub 2021 Sep 21.

Center for Biomedical Engineering and Rehabilitations, Institute for Micromanufacturing, Louisiana Tech University, PO Box 10137, Ruston, LA, 71272, USA.

Blood is an attractive carrier for plasmid and RNA-based medicine in cell therapy. Electroporation serves as a favorable delivery tool for simple operation, quick internalization, minimum cell culture involvement, and low contamination risk. However, the delivery outcome of electroporation heavily depends on the treated cells such as their type, size, and orientation to the electric field, not ideal for highly heterogeneous blood samples. Read More

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September 2021

Planar hydrodynamic traps and buried channels for bead and cell trapping and releasing.

Lab Chip 2021 09 28;21(19):3686-3694. Epub 2021 Sep 28.

Laboratory of Microsystems LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

We present a novel concept for the controlled trapping and releasing of beads and cells in a PDMS microfluidic channel without obstacles present around the particle or in the channel. The trapping principle relies on a two-level microfluidic configuration: a top main PDMS channel interconnected to a buried glass microchannel using round vias. As the fluidic resistances rule the way the liquid flows inside the channels, particles located in the streamlines passing inside the buried level are immobilized by the round with a smaller diameter, leaving the object motionless in the upper PDMS channel. Read More

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September 2021

Autoantibody profiles associated with clinical features in psychotic disorders.

Transl Psychiatry 2021 09 13;11(1):474. Epub 2021 Sep 13.

Discipline of Psychiatry, Adelaide Medical School, University of Adelaide, Adelaide, SA, Australia.

Autoimmune processes are suspected to play a role in the pathophysiology of psychotic disorders. Better understanding of the associations between auto-immunoglobulin G (IgG) repertoires and clinical features of mental illness could yield novel models of the pathophysiology of psychosis, and markers for biological patient stratification. We undertook cross-sectional detection and quantification of auto-IgGs in peripheral blood plasma of 461 people (39% females) with established psychotic disorder diagnoses. Read More

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September 2021

Droplet contact-based spheroid transfer technique as a multi-step assay tool for spheroid arrays.

Lab Chip 2021 Sep 13. Epub 2021 Sep 13.

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Hanging drop plates and low-attachment well plates are suitable for a high throughput screening model of a spheroid, because each drop (or well) contains a single spheroid and the spheroid environment are separated from each other. However, uniform spheroid culture on these devices is difficult as the liquid around the spheroid is replaced by direct pipetting, which can cause spheroid damage or loss, and well-to-well variation. If spheroids need to be cultured for a long time or analyzed through chemical treatment of immunostaining, it becomes a more considerable problem as the number of pipetting action increases. Read More

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September 2021

Graphene Oxide-Supported Microwell Grids for Preparing Cryo-EM Samples with Controlled Ice Thickness.

Adv Mater 2021 Sep 12:e2102991. Epub 2021 Sep 12.

School of Chemical and Biological Engineering, and Institute of Chemical Processes (ICP), Seoul National University, Seoul, 08826, Republic of Korea.

Cryogenic-electron microscopy (cryo-EM) is the preferred method to determine 3D structures of proteins and to study diverse material systems that intrinsically have radiation or air sensitivity. Current cryo-EM sample preparation methods provide limited control over the sample quality, which limits the efficiency and high throughput of 3D structure analysis. This is partly because it is difficult to control the thickness of the vitreous ice that embeds specimens, in the range of nanoscale, depending on the size and type of materials of interest. Read More

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September 2021

Glycosylation reduces the glycan-independent immunomodulatory effect of recombinant Orysata lectin in Drosophila S2 cells.

Sci Rep 2021 09 9;11(1):17958. Epub 2021 Sep 9.

Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, Belgium.

Several plant lectins, or carbohydrate-binding proteins, interact with glycan moieties on the surface of immune cells, thereby influencing the immune response of these cells. Orysata, a mannose-binding lectin from rice, has been reported to exert immunomodulatory activities on insect cells. While the natural lectin is non-glycosylated, recombinant Orysata produced in the yeast Pichia pastoris (YOry) is modified with a hyper-mannosylated N-glycan. Read More

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September 2021

A Review of Recent Advances in Fractional-Order Sensing and Filtering Techniques.

Sensors (Basel) 2021 Sep 2;21(17). Epub 2021 Sep 2.

Automation Department, Technical University of Cluj-Napoca, 400114 Cluj-Napoca, Romania.

The present manuscript aims at raising awareness of the endless possibilities of fractional calculus applied not only to system identification and control engineering, but also into sensing and filtering domains. The creation of the device has enabled the physical realization of a new array of sensors capable of gathering more information. The same fractional-order electronic component has led to the possibility of exploring analog filtering techniques from a practical perspective, enlarging the horizon to a wider frequency range, with increased robustness to component variation, stability and noise reduction. Read More

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September 2021

Precise and convenient size barcode on microfluidic chip for multiplex biomarker detection.

Analyst 2021 Sep 27;146(19):5892-5897. Epub 2021 Sep 27.

School of Electronic and Electrical Engineering, Wuhan Textile University, Wuhan 430200, People's Republic of China.

The existing multiplex biomarker detection methods are limited by the high demand for coding material and expensive detection equipment. This paper proposes a convenient and precise coding method based on a wedge-shaped microfluidic chip, which can be further applied in multiplex biomarker detection. The proposed microfluidic chip has a microchannel with continuously varying height, which can naturally separate and code microparticles of different sizes. Read More

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September 2021