416 results match your criteria klf6 expression

Distinct transcription factor networks control neutrophil-driven inflammation.

Nat Immunol 2021 Jul 19. Epub 2021 Jul 19.

Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.

Neutrophils display distinct gene expression patters depending on their developmental stage, activation state and tissue microenvironment. To determine the transcription factor networks that shape these responses in a mouse model, we integrated transcriptional and chromatin analyses of neutrophils during acute inflammation. We showed active chromatin remodeling at two transition stages: bone marrow-to-blood and blood-to-tissue. Read More

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Tumor-Derived Extracellular Vesicles Promote Activation of Carcinoma-Associated Fibroblasts and Facilitate Invasion and Metastasis of Ovarian Cancer by Carrying miR-630.

Front Cell Dev Biol 2021 30;9:652322. Epub 2021 Jun 30.

Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Ovarian cancer (OC) is a lethal gynecological malignancy. Extracellular vesicles (EVs) are crucial media in cell-to-cell communication by carrying microRNAs (miRs). The current study aims to investigate the underlying mechanism of miR-630 carried by OC cell-derived EVs in regard to invasion and metastasis of OC cells. Read More

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Analysis of differentially expressed genes responsible for the suppressive effect of anisomycin on cell proliferation of DLD-1 cells.

Biochem Biophys Rep 2021 Sep 5;27:101038. Epub 2021 Jun 5.

Department of Analytical Biochemistry, School of Pharmacy, Iwate Medical University, 1-1-1, Idaidori, Yahaba, Shiwa-gun, Iwate, 0283694, Japan.

Anisomycin is used as a chemical compound that possesses c-Jun N-terminal kinase (JNK)-activating effects. Recently, the potent anti-tumor effects of anisomycin have received much attention. In addition to its JNK-activating effects, anisomycin has been reported to affect gene expression in osteosarcoma, leukemia, hepatocellular carcinoma, ovarian cancer and other cancers. Read More

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September 2021

Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage Sirtuin 5/Nrf2/HO-1 Axis.

Front Aging Neurosci 2021 3;13:646729. Epub 2021 Jun 3.

Shenzhen Beike Biotechnology Research Institute, Shenzhen, China.

As a severe neurological deficit, intracerebral hemorrhage (ICH) is associated with overwhelming mortality. Subsequent oxidative stress and neurological dysfunction are likely to cause secondary brain injury. Therefore, this study sought to define the role of Krüppel-like factor 6 (KLF6) and underlying mechanism in oxidative stress and neurological dysfunction following ICH. Read More

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MiR-200b is upregulated in plasma-derived exosomes and functions as an oncogene by promoting macrophage M2 polarization in ovarian cancer.

J Ovarian Res 2021 Jun 2;14(1):74. Epub 2021 Jun 2.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Donghu District, 330006, Nanchang, Jiangxi, China.

Background: Ovarian cancer is the seventh most common cancer in women and the second most reason of gynecologic cancer-related death. Growing evidence showed that exosomal miRNA plays a crucial role in the progression of ovarian cancer.

Methods: Exosomes were identified using nanoparticle tracking analysis, transmission electron microscopy and marker proteins detection. Read More

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Krüppel-like factor 6-mediated loss of BCAA catabolism contributes to kidney injury in mice and humans.

Proc Natl Acad Sci U S A 2021 Jun;118(23)

Division of Nephrology, Department of Medicine, Stony Brook University, Stony Brook, NY 11794;

Altered cellular metabolism in kidney proximal tubule (PT) cells plays a critical role in acute kidney injury (AKI). The transcription factor Krüppel-like factor 6 (KLF6) is rapidly and robustly induced early in the PT after AKI. We found that PT-specific knockdown ( ) is protective against AKI and kidney fibrosis in mice. Read More

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Predictive value of transcriptional expression of Krüppel-like factor-6 (KLF6) in head and neck carcinoma patients treated with radiotherapy.

Clin Transl Oncol 2021 Jun 1. Epub 2021 Jun 1.

Genomics of Complex Diseases, Research Institute Hospital Sant Pau, IIB Sant Pau, Barcelona, Spain.

Purpose: To analyse the relationship between the transcriptional expression of Krüppel-like factor-6 (KLF6) and local response to treatment with radiotherapy in patients with head and neck squamous cell carcinoma (HNSCC).

Methods: We determined the transcriptional expression of KLF6 in tumour biopsies obtained before treatment with radiotherapy in 83 HNSCC patients. The KLF6 expression was categorized according to the local control of the disease with a recursive partitioning analysis. Read More

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NRF2-mediated signaling is a master regulator of transcription factors in bovine granulosa cells under oxidative stress condition.

Cell Tissue Res 2021 May 19. Epub 2021 May 19.

Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.

Transcription factors (TFs) are known to be involved in regulating the expression of several classes of genes during folliculogenesis. However, the regulatory role of TFs during oxidative stress (OS) is not fully understood. The current study was aimed to investigate the regulation of the TFs in bovine granulosa cells (bGCs) during exposure to OS induced by HO in vitro. Read More

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Co-occupancy identifies transcription factor co-operation for axon growth.

Nat Commun 2021 05 5;12(1):2555. Epub 2021 May 5.

Department of Biomedical Sciences, Marquette University, Milwaukee, WI, USA.

Transcription factors (TFs) act as powerful levers to regulate neural physiology and can be targeted to improve cellular responses to injury or disease. Because TFs often depend on cooperative activity, a major challenge is to identify and deploy optimal sets. Here we developed a bioinformatics pipeline, centered on TF co-occupancy of regulatory DNA, and used it to predict factors that potentiate the effects of pro-regenerative Klf6 in vitro. Read More

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RNA-Seq Reveals Function of Bta-miR-149-5p in the Regulation of Bovine Adipocyte Differentiation.

Animals (Basel) 2021 Apr 22;11(5). Epub 2021 Apr 22.

College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Intramuscular fat is a real challenge for the experts of animal science to improve meat quality traits. Research on the mechanism of adipogenesis provides invaluable information for the improvement of meat quality traits. This study investigated the effect of bta-miR-149-5p and its underlying mechanism on lipid metabolism in bovine adipocytes. Read More

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PVT1 knockdown inhibited the biological behavior of LPS-induced cardiac fibroblasts by regulating miR-24.

Genes Genomics 2021 Apr 27. Epub 2021 Apr 27.

Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyushan South Road, Xincheng District, Xinjiang Uygur Autonomous Region, Urumqi, 830054, China.

Background: The heart is one of the target organs vulnerable to sepsis. About 50% of sepsis patients will suffer from myocardial injury and cardiac dysfunction, which will aggravate the sepsis and affect its prognosis.

Objectives: Here, we attempt to investigate the function of long non coding RNA PVT1 in LPS-induced cardiac fibroblasts in vitro, and explore its potential mechanism. Read More

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The protective effects of azilsartan against oscillatory shear stress-induced endothelial dysfunction and inflammation are mediated by KLF6.

J Biochem Mol Toxicol 2021 Jun 1;35(6):1-8. Epub 2021 Apr 1.

Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.

Background And Purpose: Atherosclerosis is a common cardiovascular disease with high morbidity and mortality. It is reported to be related to oscillatory shear stress (OSS)-induced endothelial dysfunction and excessive production of inflammatory factors. Azilsartan, a specific antagonist of the angiotensin II receptor, has been approved for the management of hypertensive subjects with diabetes mellitus type II (DMII). Read More

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Quaking 5 suppresses TGF-β-induced EMT and cell invasion in lung adenocarcinoma.

EMBO Rep 2021 06 26;22(6):e52079. Epub 2021 Mar 26.

Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.

Quaking (QKI) proteins belong to the signal transduction and activation of RNA (STAR) family of RNA-binding proteins that have multiple functions in RNA biology. Here, we show that QKI-5 is dramatically decreased in metastatic lung adenocarcinoma (LUAD). QKI-5 overexpression inhibits TGF-β-induced epithelial-mesenchymal transition (EMT) and invasion, whereas QKI-5 knockdown has the opposite effect. Read More

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Meiotic nuclear divisions 1 (MND1) fuels cell cycle progression by activating a KLF6/E2F1 positive feedback loop in lung adenocarcinoma.

Cancer Commun (Lond) 2021 Jun 18;41(6):492-510. Epub 2021 Mar 18.

Department of Thoracic Surgery, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, Jiangsu, 210009, P. R. China.

Background: Considering the increase in the proportion of lung adenocarcinoma (LUAD) cases among all lung cancers and its considerable contribution to cancer-related deaths worldwide, we sought to identify novel oncogenes to provide potential targets and facilitate a better understanding of the malignant progression of LUAD.

Methods: The results from the screening of transcriptome and survival analyses according to the integrated Gene Expression Omnibus (GEO) datasets and The Cancer Genome Atlas (TCGA) data were combined, and a promising risk biomarker called meiotic nuclear divisions 1 (MND1) was selectively acquired. Cell viability assays and subcutaneous xenograft models were used to validate the oncogenic role of MND1 in LUAD cell proliferation and tumor growth. Read More

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Identifying loci with different allele frequencies among cases of eight psychiatric disorders using CC-GWAS.

Nat Genet 2021 04 8;53(4):445-454. Epub 2021 Mar 8.

Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, MA, USA.

Psychiatric disorders are highly genetically correlated, but little research has been conducted on the genetic differences between disorders. We developed a new method (case-case genome-wide association study; CC-GWAS) to test for differences in allele frequency between cases of two disorders using summary statistics from the respective case-control GWAS, transcending current methods that require individual-level data. Simulations and analytical computations confirm that CC-GWAS is well powered with effective control of type I error. Read More

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Febuxostat Prevents the Cytotoxicity of Propofol in Brain Endothelial Cells.

ACS Omega 2021 Mar 15;6(8):5471-5478. Epub 2021 Feb 15.

Department of Anesthesiology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Shenyang 110042, Liaoning Province, PR China.

: A high risk of brain injury has been reported with the usage of general anesthetics such as propofol in infants. Experimental data indicated that oxidative stress and inflammation are involved in the neurotoxicity induced by propofol. Febuxostat is a novel anti-gout agent recently reported to exert an anti-inflammatory effect. Read More

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Promotion of corticospinal tract growth by KLF6 requires an injury stimulus and occurs within four weeks of treatment.

Exp Neurol 2021 May 14;339:113644. Epub 2021 Feb 14.

Department of Biomedical Sciences, Marquette University, Milwaukee, WI 53233, USA. Electronic address:

Axons in the corticospinal tract (CST) display a limited capacity for compensatory sprouting after partial spinal injuries, potentially limiting functional recovery. Forced expression of a developmentally expressed transcription factor, Krüppel-like factor 6 (KLF6), enhances axon sprouting by adult CST neurons. Here, using a pyramidotomy model of injury in adult mice, we confirm KLF6's pro-sprouting properties in spared corticospinal tract neurons and show that this effect depends on an injury stimulus. Read More

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Exploring Human Skin Aging at the Single-Cell Level.

Dev Cell 2021 Feb;56(3):253-254

Immunology Discovery, Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA. Electronic address:

In this issue of Developmental Cell, Zou et al. utilize eyelid samples to examine human skin aging at the single-cell level. They discover photo- and inflammation-related changes already in middle age and find that restoring youthful expression of KLF6 and HES1 may dial back some age-associated changes. Read More

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February 2021

lncRNA-Xist/miR-101-3p/KLF6/C/EBPα axis promotes TAM polarization to regulate cancer cell proliferation and migration.

Mol Ther Nucleic Acids 2021 Mar 10;23:536-551. Epub 2020 Dec 10.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang City, 110122 Liaoning Province, China.

The phenotypic switch in tumor-associated macrophages (TAMs) mediates immunity escape of cancer. However, the underlying mechanisms in the TAM phenotypic switch have not been systematically elucidated. In this study, long noncoding RNA (lncRNA)-Xist, CCAAT/enhancer-binding protein (C/EBP)α, and Kruppel-like factor 6 (KLF6) were upregulated, whereas microRNA (miR)-101 was downregulated in M1 macrophages-type (M1). Read More

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Circular RNA CircMTO1 suppressed proliferation and metastasis of osteosarcoma through miR-630/KLF6 axis.

Eur Rev Med Pharmacol Sci 2021 01;25(1):86-93

Department of Orthopaedics, Hong Hui Hospital affiliated to Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.

Objective: Circular RNAs (circRNAs) could regulate gene expression which may induce tumor occurrence and progression. In the current study, we first investigated the expression of circMTO1 in osteosarcoma, and the underlying mechanism was further elucidated.

Patients And Methods: Circular RNA microarrays were used to identify the differential expression of circRNAs in osteosarcoma tissues and the corresponding normal tissues. Read More

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January 2021

lncRNA MIR4435-2HG promoted clear cell renal cell carcinoma malignant progression via miR-513a-5p/KLF6 axis.

J Cell Mol Med 2020 09 30;24(17):10013-10026. Epub 2020 Jul 30.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Long non-coding RNAs (lncRNAs) take various biological effects in clear cell renal cell carcinoma (ccRCC) mostly through sponging with microRNAs (miRNAs). lncRNA MIR4435-2HG is found to promote tumour progression in gastric cancer, glioblastoma and hepatocellular carcinoma. However, the role of lncRNA MIR4435-2HG in ccRCC progression remains unknown. Read More

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September 2020

SP1-induced long non-coding RNA SNHG6 facilitates the carcinogenesis of chondrosarcoma through inhibiting KLF6 by recruiting EZH2.

Cell Death Dis 2021 01 11;12(1):59. Epub 2021 Jan 11.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, P.R. China.

Small nucleolar RNA host gene 6 (SNHG6) is a newly discovered long non-coding RNA (lncRNA), while the regulatory mechanism of SNHG6 in chondrosarcoma is largely unknown. Here we found that SNHG6 expression was upregulated and showed positive correlation with the progression of chondrosarcoma. Functional assays demonstrated that SNHG6 was required for the proliferation, migration, and invasion of chondrosarcoma cells. Read More

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January 2021

Cell autonomous angiotensin II signaling controls the pleiotropic functions of oncogenic K-Ras.

J Biol Chem 2021 Jan-Jun;296:100242. Epub 2021 Jan 8.

Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA. Electronic address:

Oncogenic K-Ras (K-Ras) promotes senescence in normal cells but fuels transformation of cancer cells after the senescence barrier is bypassed. The mechanisms regulating this pleiotropic function of K-Ras remain to be fully established and bear high pathological significance. We find that K-Ras activates the angiotensinogen (AGT) gene promoter and promotes AGT protein expression in a Kruppel-like factor 6-dependent manner in normal cells. Read More

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January 2021

MicroRNA-2355-5p regulates γ-globin expression in human erythroid cells by inhibiting KLF6.

Br J Haematol 2021 Apr 24;193(2):401-405. Epub 2020 Dec 24.

Department of Medical Genetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.

Krüppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised γ-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34 ) cells. Read More

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Maternal undernutrition modulates hepatic MicroRNAs expression in the early life of offspring.

Exp Cell Res 2021 Mar 19;400(2):112450. Epub 2020 Dec 19.

Department of Endocrinology, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address:

Emerging studies revealed that a poor intrauterine environment elicited by maternal nutrient restriction (MNR) is associated with an increased risk of metabolic diseases in adulthood. Previous research has shown that microRNAs (miRNAs) exert pivotal roles in modulating molecular pathways involved in disease pathogenesis and progression. In this respect, we herein examined miRNA profiles in samples of liver from offspring whose mothers were fed either with a 50% food-restricted diet or standard laboratory chow during pregnancy. Read More

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A Single-Cell Transcriptomic Atlas of Human Skin Aging.

Dev Cell 2021 Feb 24;56(3):383-397.e8. Epub 2020 Nov 24.

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Skin undergoes constant self-renewal, and its functional decline is a visible consequence of aging. Understanding human skin aging requires in-depth knowledge of the molecular and functional properties of various skin cell types. We performed single-cell RNA sequencing of human eyelid skin from healthy individuals across different ages and identified eleven canonical cell types, as well as six subpopulations of basal cells. Read More

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February 2021

ZZEF1 is a Histone Reader and Transcriptional Coregulator of Krüppel-Like Factors.

J Mol Biol 2021 01 21;433(2):166722. Epub 2020 Nov 21.

Center for Epigenetics, Van Andel Institute, Grand Rapids, MI 49503, USA. Electronic address:

The ZZ-type zinc finger and EF-hand domain protein 1 (ZZEF1) is a multidomain-containing protein. Mutations of ZZEF1 has been implicated in several kinds of human diseases such as diabetes and cancers. However, the function of the ZZEF protein remains largely unknown. Read More

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January 2021

Krüppel-like factor 6 promotes odontoblastic differentiation through regulating the expression of dentine sialophosphoprotein and dentine matrix protein 1 genes.

Int Endod J 2021 Apr 16;54(4):572-584. Epub 2021 Jan 16.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.

Aim: To investigate the potential role of Krüppel-like factor 6 (KLF6) in the odontoblastic differentiation of immortalized dental papilla mesenchymal cells (iMDP-3) cells.

Methodology: Alizarin Red S (ARS) and Alkaline phosphatase (ALP) staining was used to examine the mineralization effect of iMDP-3 cells after odontoblastic induction. Real-time PCR and Western blotting were employed to analyse dentine sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP1), RUNX family transcription factor 2 (RUNX2), ALP and KLF6 expression during this process. Read More

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Single-Cell Transcriptome Analysis Highlights a Role for Neutrophils and Inflammatory Macrophages in the Pathogenesis of Severe COVID-19.

Cells 2020 10 29;9(11). Epub 2020 Oct 29.

College of Health & Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), PO Box 34110, Doha, Qatar.

Cumulative data link cytokine storms with coronavirus disease 2019 (COVID-19) severity. The precise identification of immune cell subsets in bronchoalveolar lavage (BAL) and their correlation with COVID-19 disease severity are currently being unraveled. Herein, we employed iterative clustering and guide-gene selection 2 (ICGS2) as well as uniform manifold approximation and projection (UMAP) dimensionality reduction computational algorithms to decipher the complex immune and cellular composition of BAL, using publicly available datasets from a total of 68,873 single cells derived from two healthy subjects, three patients with mild COVID-19, and five patients with severe COVID-19. Read More

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October 2020

Apremilast ameliorates ox-LDL-induced endothelial dysfunction mediated by KLF6.

Aging (Albany NY) 2020 Oct 14;12(19):19012-19021. Epub 2020 Oct 14.

Department of General Practice, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.

Apremilast is a phosphodiesterase 4 (PDE4) inhibitor used in the treatment of psoriasis and several other inflammatory diseases. Interest has been expressed in seeking out therapies that address both psoriasis and atherosclerosis. In the present study, we explored the effects of apremilast in human aortic endothelial cells (HAECs) exposed to oxidized low-density lipoprotein (ox-LDL) to simulate the atherosclerotic microenvironment . Read More

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October 2020