19,142 results match your criteria k562 cells



J Cell Biochem 2021 Jul 20;122(7):771. Epub 2021 Apr 20.

Retraction: "MicroRNA-103 confers the resistance to long-treatment of adriamycin to human leukemia cells by regulation of COP1," by Lin Wan, Yanlong Tian, Rui Zhang, Zhuo Peng, Jiangli Sun, and Wanggang Zhang, J Cell Biochem. 2018; 3843-3852: The above article, published online on 23 Oct 2017 in Wiley Online Library (https://onlinelibrary.wiley. Read More

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Combining AFM13, a bispecific CD30/CD16 antibody, with cytokine-activated cord blood-derived NK cells facilitates CAR-like responses against CD30+ malignancies.

Clin Cancer Res 2021 May 13. Epub 2021 May 13.

Institute of Bioinformatics & IBM Bio-computational Laboratory, Zhejiang University, China.

Purpose: Natural killer (NK) cell recognition and function against NK-resistant cancers remains substantial barriers to the broad application of NK cell immunotherapy. Potential solutions include bispecific engagers that target NK cell activity via an NK activating receptor when simultaneously targeting a tumor-specific antigen, as well as enhancing functionality using IL-12/15/18 cytokine pre-activation.

Experimental Design: We assessed single-cell NK cell responses stimulated by the tetravalent bispecific antibody AFM13 that binds CD30 on leukemia/lymphoma targets and CD16A on various types of NK cells using mass cytometry and cytotoxicity assays. Read More

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AKR1C3 decreased CML sensitivity to Imatinib in bone marrow microenvironment via dysregulation of miR-379-5p.

Cell Signal 2021 May 10:110038. Epub 2021 May 10.

Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu, China. Electronic address:

Background: Drug resistance is an important cause of death for most patients with chronic myeloid leukemia (CML). The bone marrow microenvironment is believed to be mainly responsible for resistance to BCR-ABL tyrosine kinase inhibitors. The mechanism involved, however, is still unclear. Read More

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Expanded natural killer cells augment the antimyeloma effect of daratumumab, bortezomib, and dexamethasone in a mouse model.

Cell Mol Immunol 2021 May 12. Epub 2021 May 12.

Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital and Chonnam National University Medical School, Gwangju, Korea.

The use of natural killer (NK) cells is a promising and safe immunotherapeutic approach in the field of cancer immunotherapy. However, combination treatments are required to enhance the effector functions and therapeutic efficacy of NK cells. In this study, we investigated the potential of daratumumab (Dara), bortezomib, and dexamethasone (Dvd) to augment the antitumor effects of NK cells in a multiple myeloma (MM) xenograft mouse model. Read More

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Metabolomics and Proteomics Reveal the Variation of Substances in Apheresis Platelets during Storage and Their Effects on Cancer Cell Proliferation.

Transfus Med Hemother 2021 Mar 25;48(2):79-90. Epub 2020 Sep 25.

Department of Blood Transfusion, Chinese PLA General Hospital, Beijing, China.

Introduction: Apheresis platelets (APs) are clinically and crucially important in the prevention and treatment of bleeding in patients with thrombocytopenia or cancer. However, few researchers have addressed the variation of supernatant metabolites and exosome proteins in APs during storage and their effects on cancer cell proliferation.

Objective: This study was designed to explore the change rules of the metabolites and exosomal proteins of APs during storage and their effects on cancer cell proliferation. Read More

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Relation between ABCB1 overexpression and COX2 and ALOX5 genes in human erythroleukemia cell lines.

Prostaglandins Other Lipid Mediat 2021 May 8:106553. Epub 2021 May 8.

Laboratório de Cultura Celular, ICB, FURG, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas, ICB, FURG, RS, Brazil. Electronic address:

This study aimed to characterize the relationship between the COX2 and ALOX5 genes, as well as their link with the multidrug resistance (MDR) phenotype in sensitive (K562) and MDR (K562-Lucena and FEPS) erythroleukemia cells. For this, the inhibitors of 5-LOX (zileuton) and COX-2 (acetylsalicylic acid-ASA) and cells with the silenced ABCB1 gene were used. The treatment with ASA caused an increase in the gene expression of COX2 and ABCB1 in both MDR cell lines, and a decrease in the expression of ALOX5 in the FEPS cells. Read More

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Optimization of CUT&Tag product recovery and library construction method.

Yi Chuan 2021 Apr;43(4):362-374

Novoprotein Scientific Inc., Wujiang 215200, China.

The emerging cleavage under target and tagment (CUT&Tag) technology uses Tn5 transposase to cleavage near the DNA binding site of target protein and study the generated DNA fragments by the next-generation sequencing. It can quickly identify protein-DNA interactions, which greatly simplifies the experimental process of ChIP-Seq. After CUT&Tag tagment reaction, DNA recovery or other post-processing is required to perform library construction PCR. Read More

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Extract Suppress Glioblastoma Growth through Promotion of Genotoxicity and Apoptosis: and Studies.

Int J Med Sci 2021 22;18(11):2417-2430. Epub 2021 Apr 22.

Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan, ROC.

Glioblastoma (GBM) is the most common malignant primary brain tumor in humans, exhibiting highly infiltrative growth and drug resistance to conventional chemotherapy. (CAt) extract has been shown to decrease postoperative pain and inhibit the growth of K562 leukemia cells. The aim of this study was to assess the anti-GBM activity and molecular mechanism of CAt extract and . Read More

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Investigation of water-insoluble hydrophobic polyethylenimines as RNAi vehicles in chronic myeloid leukemia therapy.

J Biomed Mater Res A 2021 May 8. Epub 2021 May 8.

Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Canada.

The discovery of RNA interference (RNAi) more than two decades ago opened avenues for avant-garde cancer treatments that possess the ability to evade issues hampering current chemotherapeutic strategies, owing to its specific gene sequence-driven mechanism of action. A potent short interfering RNA (siRNA) delivery vehicle designed to overcome physiological barriers is imperative for successful RNAi therapy. For this purpose, this study explored the characteristics and therapeutic efficacy of low-molecular weight (MW) polyethylenimine (PEI) with high cholesterol substitution, yielding water-insoluble polymers, in chronic myeloid leukemia (CML) K562 cells. Read More

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Prodigiosin induced the caspase-dependent apoptosis in human chronic myelogenous leukemia K562 cell.

Res Pharm Sci 2021 Feb 30;16(1):26-34. Epub 2020 Dec 30.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, I.R. Iran.

Background And Purpose: Chronic myeloid leukemia (CML) as a myeloproliferative disease is characterized by increased cellularity of bone marrow. Implementing the latest treatment protocols is currently accompanied by serious and life-threatening side effects. There are worldwide attempts to find new effective and potent therapeutic agents with minimal side effects on CML patients. Read More

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February 2021

Sialic acid-binding immunoglobulin-like lectin (Siglec)-15 is a rapidly internalised cell-surface antigen expressed by acute myeloid leukaemia cells.

Br J Haematol 2021 May 5. Epub 2021 May 5.

Department of Microbiology and Immunology (DMI), The University of Melbourne, Melbourne, Australia.

Sialic acid-binding immunoglobulin-like lectin (Siglec)-15 has recently been identified as a critical tumour checkpoint, augmenting the expression and function of programmed death-ligand 1. We raised a monoclonal antibody, A9E8, specific for Siglec-15 using phage display. A9E8 stained myeloid leukaemia cell lines and peripheral cluster of differentiation (CD)33 blasts and CD34 leukaemia stem cells from patients with acute myeloid leukaemia (AML). Read More

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Ampelopsin Inhibits Cell Proliferation and Induces Apoptosis in HL60 and K562 Leukemia Cells by Downregulating AKT and NF-κB Signaling Pathways.

Int J Mol Sci 2021 Apr 20;22(8). Epub 2021 Apr 20.

Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea.

Leukemia is a type of blood cancer caused by the rapid proliferation of abnormal white blood cells. Currently, several treatment options, including chemotherapy, radiation therapy, and bone marrow transplantation, are used to treat leukemia, but the morbidity and mortality rates of patients with leukemia are still high. Therefore, there is still a need to develop more selective and less toxic drugs for the effective treatment of leukemia. Read More

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Quantification of Intracellular Thiols by HPLC-Fluorescence Detection.

Molecules 2021 Apr 19;26(8). Epub 2021 Apr 19.

Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 1130033, Japan.

Biothiols, such as cysteine and glutathione, play important roles in various intracellular reactions represented by the redox equilibrium against oxidative stress. In this study, a method for intracellular thiol quantification using HPLC-fluorescence detection was developed. Thiols were derivatized with a thiol-specific fluorescence derivatization reagent, viz. Read More

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Hypoxia-Driven HIF-1α Activation Reprograms Pre-Activated NK Cells towards Highly Potent Effector Phenotypes via ERK/STAT3 Pathways.

Cancers (Basel) 2021 Apr 15;13(8). Epub 2021 Apr 15.

Department of Biochemistry and Molecular Biology, College of Medicine, Korea University, Seoul 02841, Korea.

NK cells are the predominant innate lymphocyte subsets specialized to kill malignant tumor cells. In patients with advanced cancer, hypoxic stress shapes NK cells toward tumor-resistant and immunosuppressive phenotypes, hence a strategy to restore NK function is critical for successful tumor immunotherapy. Here, we present evidence that pre-activation and subsequent HIF-1α-dependent metabolic shift of NK cells from oxidative phosphorylation into glycolysis are keys to overcome hypoxia-mediated impairment in NK cell survival, proliferation, and tumor cytotoxicity. Read More

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Natural Trienoic Acids as Anticancer Agents: First Stereoselective Synthesis, Cell Cycle Analysis, Induction of Apoptosis, Cell Signaling and Mitochondrial Targeting Studies.

Cancers (Basel) 2021 Apr 10;13(8). Epub 2021 Apr 10.

Institute of Petrochemistry and Catalysis, Russian Academy of Sciences pr. Oktyabrya 141, 450075 Ufa, Russia.

The first Z-stereoselective method was developed for the synthesis of unsaturated acids containing a 1Z,5Z,9Z-triene moiety in 61-64% yields using the new Ti-catalyzed cross-coupling of oxygen-containing and aliphatic 1,2-dienes as the key synthetic step. It was shown for the first time that trienoic acids with non-methylene-interrupted Z-double bonds show moderate cytotoxic activities against tumor cell lines (Jurkat, K562, U937, HL60, HeLa), human embryonic kidney cells (Hek293), normal fibroblasts and human topoisomerase I (hTop1) inhibitory activity in vitro. The synthesized acids efficiently initiate apoptosis of Jurkat tumor cells, with the cell death mechanism being activated by the mitochondrial pathway. Read More

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New Succinimides with Potent Anticancer Activity: Synthesis, Activation of Stress Signaling Pathways and Characterization of Apoptosis in Leukemia and Cervical Cancer Cells.

Int J Mol Sci 2021 Apr 21;22(9). Epub 2021 Apr 21.

Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Polish Academy of Sciences, 112 Sienkiewicza, 90-363 Lodz, Poland.

Based on previously identified dicarboximides with significant anticancer and immunomodulatory activities, a series of 26 new derivatives were designed and synthesized by the Diels-Alder reaction between appropriate diene and maleimide or hydroxymaleimide moieties. The resulting imides were functionalized with alkanolamine or alkylamine side chains and subsequently converted to their hydrochlorides. The structures of the obtained compounds were confirmed by 1H and 13C NMR and by ESI MS spectral analysis. Read More

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Combination of PKCδ Inhibition with Conventional TKI Treatment to Target CML Models.

Cancers (Basel) 2021 Apr 2;13(7). Epub 2021 Apr 2.

Université Côte d'Azur, Institut National de la Santé et de la Recherche Médicale (Inserm) U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), 06204 Nice, France.

Numerous combinations of signaling pathway blockades in association with tyrosine kinase inhibitor (TKI) treatment have been proposed for eradicating leukemic stem cells (LSCs) in chronic myeloid leukemia (CML), but none are currently clinically available. Because targeting protein kinase Cδ (PKCδ) was demonstrated to eliminate cancer stem cells (CSCs) in solid tumors, we evaluated the efficacy of PKCδ inhibition in combination with TKIs for CML cells. We observed that inhibition of PKCδ by a pharmacological inhibitor, by gene silencing, or by using K562 CML cells expressing dominant-negative (DN) or constitutively active (CA) PKCδ isoforms clearly points to PKCδ as a regulator of the expression of the stemness regulator BMI1. Read More

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Isomalabaricane Triterpenes from the Marine Sponge sp.

Mar Drugs 2021 Apr 6;19(4). Epub 2021 Apr 6.

National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan.

The marine sponge of the genus , , , and are characterized chemically by a variety of isomalabaricane triterpenes. This class of compounds drew spotlights in marine lead discovery due to their profound anti-proliferative properties. Further research on exploring its chemical diversity led to the identifications of two new isomalabaricane-type triterpenes rhabdastin H () and rhabdastin I (). Read More

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Photocytotoxic Activity of Ruthenium(II) Complexes with Phenanthroline-Hydrazone Ligands.

Molecules 2021 Apr 6;26(7). Epub 2021 Apr 6.

Departamento de Química, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Belo Horizonte (MG) 31270-901, Brazil.

This paper reports on the synthesis and characterization of two new polypyridyl-hydrazone Schiff bases, ()-'-(6-oxo-1,10-phenanthrolin-5(6)-ylidene)thiophene-2-carbohydrazide () and ()-'-(6-oxo-1,10-phenanthrolin-5(6)-ylidene)furan-2-carbohydrazide (), and their two Ru(II) complexes of the general formula [RuCl(DMSO)(phen)(L)](PF6). Considering that hydrazides are a structural part of severa l drugs and metal complexes containing phenanthroline derivatives are known to interact with DNA and to exhibit antitumor activity, more potent anticancer agents can be obtained by covalently linking the thiophene acid hydrazide or the furoic acid hydrazide to a 1,10-phenanthroline moiety. These ligands and the Ru(II) complexes were characterized by elemental analyses, electronic, vibrational, H NMR, and ESI-MS spectroscopies. Read More

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[CRISPR/Cas9-mediated microRNA-21 knockout increased imatinib sensitivity in chronic myeloid leukemia cells].

Zhonghua Xue Ye Xue Za Zhi 2021 Mar;42(3):243-249

Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fuzhou 350001, China.

To observe the effects of miR-21 knockout on proliferation and drug resistance in K562/G01 cells, and to preliminarily explore the mechanism of imatinib sensitivity by knocking out miR-21 in K562/G01 cells. Using CRISPR/Cas9 to knock out the miR-21 gene in K562/G01 cells, and single-cell-derived clones of miR-21 knockout were obtained by genomic DNA PCR screening, Sanger sequencing, and real-time PCR. We used MTT and cell colony formation assays to assess the cell proliferation, and determined imatinib sensitivity by MTT assay and Annexin-Ⅴ-APC/7-AAD double staining flow cytometry. Read More

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The Apoptotic Properties of Leaf Extracts of Simarouba glauca against Human Leukemic Cancer Cells.

Asian Pac J Cancer Prev 2021 Apr 1;22(4):1305-1312. Epub 2021 Apr 1.

Department of Dentistry, Oral Health Institute, Hamad Medical corporation, Doha, Qatar.

Background And Objective: Simarouba glauca is a plant belonging to the family of Simaroubaceae. It is a potent source of secondary metabolites. The aim of this study was to evaluate the apoptotic properties of leaf extracts of Simarouba glauca against human leukemic cancer cells. Read More

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Chromatin occupancy and target genes of the haematopoietic master transcription factor MYB.

Sci Rep 2021 Apr 26;11(1):9008. Epub 2021 Apr 26.

Department of Biosciences, University of Oslo, Blindern, PO Box 1066, 0316, Oslo, Norway.

The transcription factor MYB is a master regulator in haematopoietic progenitor cells and a pioneer factor affecting differentiation and proliferation of these cells. Leukaemic transformation may be promoted by high MYB levels. Despite much accumulated molecular knowledge of MYB, we still lack a comprehensive understanding of its target genes and its chromatin action. Read More

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Preliminary study on the function of TMEM50A and its correlation with the RH genes.

Transfus Med 2021 Apr 25. Epub 2021 Apr 25.

Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objectives: The purpose of this study was to investigate the association and impact of TMEM50A on RH genes activity and function.

Background: SMP1 is located on chromosome 1p36.11 in the RH gene locus, between the RHD and RHCE gene, where its position may be linked to RH haplotypes and contribute to selective pressures regarding certain RH haplotypes. Read More

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CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells.

Nat Commun 2021 04 23;12(1):2437. Epub 2021 Apr 23.

Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.

CRISPR-Cas9 cytidine and adenosine base editors (CBEs and ABEs) can disrupt genes without introducing double-stranded breaks by inactivating splice sites (BE-splice) or by introducing premature stop (pmSTOP) codons. However, no in-depth comparison of these methods or a modular tool for designing BE-splice sgRNAs exists. To address these needs, we develop SpliceR ( http://z. Read More

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Leukemia Cells Resistant to Glutamine Deprivation Express Glutamine Synthetase (GS) Protein.

Turk J Haematol 2021 Apr 22. Epub 2021 Apr 22.

Istanbul Medeniyet University, Medical Faculty, Department of Medical Biochemistry, İstanbul, Turkey.

Aim: Low glutamine level has been shown in tumor environment for several cancer subtypes. Therefore, it has been suggested that cancer cells rewire their metabolism to adopt low nutrient level for survival and proliferation. Although glutamine is a non-essential amino acid and can be synthesized de novo, many cancer cells including malignant hematopoietic cells have been indicated to be addicted to glutamine. Read More

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Trienone analogs of curcuminoids induce fetal hemoglobin synthesis via demethylation at γ-globin gene promoter.

Sci Rep 2021 Apr 20;11(1):8552. Epub 2021 Apr 20.

Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

The reactivation of γ-globin chain synthesis to combine with excess free α-globin chains and form fetal hemoglobin (HbF) is an important alternative treatment for β-thalassemia. We had reported HbF induction property of natural curcuminoids, curcumin (Cur), demethoxycurcumin (DMC) and bis-demethoxycurcumin (BDMC), in erythroid progenitors. Herein, the HbF induction property of trienone analogs of the three curcuminoids in erythroleukemic K562 cell lines and primary human erythroid progenitor cells from β-thalassemia/HbE patients was examined. Read More

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Optimizing Integration and Expression of Transgenic Bruton's Tyrosine Kinase for CRISPR-Cas9-Mediated Gene Editing of X-Linked Agammaglobulinemia.

CRISPR J 2021 Apr;4(2):191-206

Department of Pediatrics, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California, USA; Departments of University of California, Los Angeles, Los Angeles, California, USA.

X-linked agammaglobulinemia (XLA) is a monogenic primary immune deficiency characterized by very low levels of immunoglobulins and greatly increased risks for recurrent and severe infections. Patients with XLA have a loss-of-function mutation in the Bruton's tyrosine kinase () gene and fail to produce mature B lymphocytes. Gene editing in the hematopoietic stem cells of XLA patients to correct or replace the defective gene should restore B cell development and the humoral immune response. Read More

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Human Wharton's Jelly Stem Cell Secretions Inhibit Human Leukemic Cell Line K562 by Inducing Cell Cycle Arrest and Apoptosis.

Front Cell Dev Biol 2021 18;9:614988. Epub 2021 Mar 18.

Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Emerging resistance to the tyrosine kinase inhibitors that target the BCR-ABL1 oncoprotein has prompted research for novel therapeutics against chronic myeloid leukemia (CML). Herein, we evaluated the tumor inhibitory properties of the human Wharton's jelly stem cells (hWJSCs) co-culture (hWJSC-CC) and their extracts, namely, the hWJSC-conditioned medium (hWJSC-CM; 100%) and hWJSC-lysate (hWJSC-L; 15 μg/ml), on a CML cell line K562 . The hWJSCs expressed mesenchymal stem cell (MSC)-related cluster of differentiation (CD) markers and demonstrated mesodermal tissue differentiation potential. Read More

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TriBAFF-CAR-T cells eliminate B-cell malignancies with BAFFR-expression and CD19 antigen loss.

Cancer Cell Int 2021 Apr 17;21(1):223. Epub 2021 Apr 17.

Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong Province, 510317, China.

Background: To investigate the effect of TriBAFF-CAR-T cells on hematological tumor cells.

Methods: TriBAFF-CAR-T and CD19-CAR-T cells were co-cultured with BAFFR-bearing B-cell malignancies at different effector/target ratios to evaluate the anti-tumor effects. In vivo, TriBAFF-CAR-T and CD19-CAR-T cells were intravenously injected into Raji-luciferase xenograft mice. Read More

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The selective cytotoxicity of silver thiosulfate, a silver complex, on MCF-7 breast cancer cells through ROS-induced cell death.

Pharmacol Rep 2021 Apr 17. Epub 2021 Apr 17.

Division of Pharmacokinetics and Pharmacodynamics, Department of Pharmacology, Toxicology and Therapeutics, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.

Background: Silver is a transition metal that is known to be less toxic than platinum. However, only few studies have reported the anticancer effects of some silver complexes and their possibility as an alternative to platinum complex. This study investigated the anticancer effects of the silver thiosulfate complex (STS), [Ag(SO)], consisting of silver and sodium thiosulfate. Read More

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