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Plasmid 2021 Mar 18;114:102554. Epub 2021 Jan 18.

Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, USA; Department of Medicine, Division of Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pharmacology, Vanderbilt University, Nashville, TN, USA. Electronic address:

TcBuster is a hAT-family DNA transposon from the red flour beetle, Tribolium castaneum. The TcBuster transposase is of interest for genome engineering as it is highly active in insect and mammalian cells. To test the predicted catalytic triad of TcBuster, each residue of the catalytic triad of a haemagglutinin-tagged TcBuster transposase was individually mutated to a structurally conserved amino acid. Read More

Nat Commun 2020 07 22;11(1):3677. Epub 2020 Jul 22.

York Computational Immunology Lab, University of York, York, UK.

Through the formation of concentration gradients, morphogens drive graded responses to extracellular signals, thereby fine-tuning cell behaviors in complex tissues. Here we show that the chemokine CXCL13 forms both soluble and immobilized gradients. Specifically, CXCL13 follicular reticular cells form a small-world network of guidance structures, with computer simulations and optimization analysis predicting that immobilized gradients created by this network promote B cell trafficking. Read More

Accid Anal Prev 2020 Jul 15;142:105563. Epub 2020 May 15.

Washington State University, United States.

The Family Climate for Road Safety Scale (FCRSS) was developed to measure parenting behaviors specific to the driving context. The original validation study found a scale structure composed of seven factors. However, this structure has not been consistently replicated. Read More

Neuroimage 2020 08 29;217:116864. Epub 2020 Apr 29.

Department of Radiology, Stanford University, Stanford, CA, 94035, USA. Electronic address:

Collegiate football athletes are subject to repeated head impacts. The purpose of this study was to determine whether this exposure can lead to changes in brain structure. This prospective cohort study was conducted with up to 4 years of follow-up on 63 football (high-impact) and 34 volleyball (control) male collegiate athletes with a total of 315 MRI scans (after exclusions: football n ​= ​50, volleyball n ​= ​24, total scans ​= ​273) using high-resolution structural imaging. Read More

Mol Cell 2019 04 27;74(2):245-253.e6. Epub 2019 Feb 27.

Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA; Committee on Higher Degrees in Biophysics, Harvard University, Cambridge, MA 02138, USA; Program in Biological and Biomedical Sciences, Harvard University, Cambridge, MA 02138, USA; Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA. Electronic address:

Transcription factors (TFs) control gene expression by binding DNA recognition sites in genomic regulatory regions. Although most forkhead TFs recognize a canonical forkhead (FKH) motif, RYAAAYA, some forkheads recognize a completely different (FHL) motif, GACGC. Bispecific forkhead proteins recognize both motifs, but the molecular basis for bispecific DNA recognition is not understood. Read More

Biomed Opt Express 2019 Feb 15;10(2):584-599. Epub 2019 Jan 15.

Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.

Deep imaging of vasculature requires small, bright, and photostable fluorophores suitable for multiphoton microscopy (MPM). Although semiconducting polymer dots (pdots) are an emerging class of highly fluorescent contrast agents with favorable advantages for the next generation of imaging, their use for deep MPM has never before been demonstrated. Herein, we characterize the multiphoton properties of three pdot variants and perform deep MPM imaging of cortical rodent microvasculature. Read More

Curr Opin Biomed Eng 2017 Dec 27;4:32-39. Epub 2017 Sep 27.

Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.

We present a review of imaging deep-tissue structures with multiphoton microscopy. We examine the effects of light scattering and absorption due to the optical properties of biological sample and identify 1,300 nm and 1,700 nm as ideal excitation wavelengths. We summarize the availability of fluorophores for multiphoton microscopy as well as ultrafast laser sources to excite available fluorophores. Read More

Neuropsychol Rehabil 2019 Sep 26;29(8):1256-1272. Epub 2017 Oct 26.

e Department of Clinical Neurosciences , University of Cambridge , Cambridge , UK.

Subjective changes are increasingly recognised as important in recovery and rehabilitation following traumatic brain injury. Accumulation of subjective changes over time has led many to examine the question of "continuity of self" post-injury. Vacillation between feeling the same and different is common and often at odds with the medical narrative preparing families for permanent change. Read More

J Virol 2017 11 27;91(22). Epub 2017 Oct 27.

Department of Genetics, University of Leicester, Leicester, United Kingdom

The genomes of human herpesvirus 6A (HHV-6A) and HHV-6B have the capacity to integrate into telomeres, the essential capping structures of chromosomes that play roles in cancer and ageing. About 1% of people worldwide are carriers of chromosomally integrated HHV-6 (ciHHV-6), which is inherited as a genetic trait. Understanding the consequences of integration for the evolution of the viral genome, for the telomere, and for the risk of disease associated with carrier status is hampered by a lack of knowledge about ciHHV-6 genomes. Read More

Bull Math Biol 2017 Oct 27;79(10):2258-2272. Epub 2017 Jul 27.

Department of Mathematics, Florida State University, 1017 Academic Way, Tallahassee, FL, 32306, USA.

We apply two different sensitivity techniques to a model of bacterial colonization of the anterior nares to better understand the dynamics of Staphylococcus aureus nasal carriage. Specifically, we use partial rank correlation coefficients to investigate sensitivity as a function of time and identify a reduced model with fewer than half of the parameters of the full model. The reduced model is used for the calculation of Sobol' indices to identify interacting parameters by their additional effects indices. Read More

JMIR Res Protoc 2015 Sep 11;4(3):e108. Epub 2015 Sep 11.

Exercise Science and Health Promotion, School of Nutrition and Health Promotion, Arizona State University, Phoenix, AZ, United States.

Background: Walking is a widely accepted and frequently targeted health promotion approach to increase physical activity (PA). Interventions to increase PA have produced only small improvements. Stronger and more potent behavioral intervention components are needed to increase time spent in PA, improve cardiometabolic risk markers, and optimize health. Read More

Medicare Medicaid Res Rev 2014 2;4(3). Epub 2014 Oct 2.

RTI International.

Background: Prior to the implementation of the Hospital-Acquired Condition-Present on Admission (HAC-POA) payment policy, concerns regarding its potential impact were raised by a number of organizations and individuals. The purpose of this study was to explore direct and indirect effects of the HAC-POA payment policy on hospitals, patients, and other payers during the policy's first 3 years of implementation.

Methods: The study included semi-structured telephone interviews with representatives of national organizations, hospitals, patient advocacy organizations, and other payers. Read More

J Fish Biol 2013 Nov;83(5):1221-33

Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Room 236D, Jarrett Building, 464 Bearsden Road, Glasgow, G61 1QH, U.K.

This study examined the potential biochemical and mechanical structures that may contribute to egg adhesion in European whitefish Coregonus lavaretus. Experiments showed that eggs from a population of C. lavaretus from Loch Eck remained non-adhesive in a solution chemically similar to ovarian fluid but became adhesive seconds after contact with water. Read More

J Pers Disord 2014 Apr 20;28(2):225-46. Epub 2013 Jun 20.

Changes in personality trait levels often parallel episodes of major depressive disorder (MDD), whereas trait factor structures and substantial retest correlations are preserved. The authors explicated this dual state/trait nature of personality assessments among adults with recurrent MDD (N = 351) receiving cognitive therapy (CT) by testing stability and change with the Schedule for Nonadaptive and Adaptive Personality, 2nd Edition (SNAP-2; Clark, Simms, Wu, & Casillas, in press), separating state and trait variance, and predicting depressive symptoms and clinical outcomes. Many SNAP scale scores changed in CT (e. Read More

Nat Prod Commun 2012 Dec;7(12):1587-8

Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.

Five flavonoids, 5-hydroxy-(6:7,3':4')-di(2,2-dimethylpyrano)flavone 1, carpachromene 2, cycloartocarpesin 3, norartocarpetin 4 and 2'-hydroxy-4,4',6'-trimethoxychalcone 5, along with three triterpenes, friedelin 6, lupeol 7 and 13-sitosterol 8 were isolated for the first time from the leaves of Artocarpus fulvicortex F.M. Jarrett. Read More

J Shoulder Elbow Surg 2011 Apr 12;20(3):477-80. Epub 2010 Oct 12.

Emory University School of Medicine Dept. of Orthopaedic Surgery, Atlanta, GA, USA.

Background: An anatomic study specifically investigating the optimal location for proximal biceps tenodesis and detailing the topographic relationship to neurovascular structures has not been conducted.

Methods: Twelve cadaveric upper extremities were dissected to identify the proximal biceps musculotendinous junction and topographic relationships to neighboring neurovascular structures.

Results: The musculotendinous junction of the long head of the biceps tendon was on average 2. Read More

J Asian Nat Prod Res 2010 Feb;12(2):106-12

Department of Chemistry, Universiti Putra Malaysia 43400 UPM, Selangor, Malaysia.

Two new xanthones, pyranocycloartobiloxanthone A (1) and dihydroartoindonesianin C (2), were isolated from the stem bark of Artocarpus obtusus Jarrett by chromatographic separation. Their structures were determined by using spectroscopic methods and comparison with known related compounds. Pyranocycloartobiloxanthone A (1) showed strong free radical scavenging activity by using DPPH assay as well as cytotoxicity towards K562, HL-60, and MCF7 cell lines. Read More

Am J Psychiatry 2007 Nov;164(11):1729-36

Sackler Institute, Weill Cornell Medical College of Cornell University, 1300 York Ave., Box 140, New York, NY 10021, USA.

Objective: Many studies have linked the structure and function of frontostriatal circuitry to cognitive control deficits in attention deficit hyperactivity disorder (ADHD). Few studies have examined the role of white matter tracts between these structures or the extent to which white matter tract myelination and regularity correlate in family members with the disorder.

Method: Functional imaging maps from a go/nogo task were used to identify portions of the ventral prefrontal cortex and striatum involved in suppressing an inappropriate action (i. Read More

Urology 2004 May;63(5):981-2

Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.

We report the first case of appendiceal mucinous cystadenoma associated with the kidney. A 57-year-old man with chronic renal failure presented with a right renal mass. During laparoscopic nephrectomy, frozen analysis of a tubular structure extending from the mass showed appendiceal tissue. Read More

J Endourol 2002 Oct;16(8):621-5

Brady Urological Institute, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA.

Background And Purpose: High-intensity focused ultrasound (HIFU) is a noninvasive technology capable of inducing thermal coagulative necrosis of subsurface structures without injuring intervening tissues. We have previously reported on the feasibility of HIFU vasectomy in a canine model. In this study, we evaluated the feasibility of HIFU ablation of the canine epididymis as an alternative to vasectomy. Read More

J Urol 2002 Jun;167(6):2613-7

Department of Pathology, Brady Urological Institute, The Johns Hopkins Medical Institutions, Baltimore, MD, USA.

Purpose: High intensity focused ultrasound is an ablative technology capable of producing thermal coagulative necrosis of sub-surface structures without injuring intervening tissues. We assessed the feasibility of using high intensity focused ultrasound to produce occlusion of the canine vas deferens.

Materials And Methods: A high intensity focused ultrasound transducer was incorporated into a hand held clip specially designed to grasp the vas deferens transcutaneously. Read More

Cancer Res 2001 Nov;61(21):7978-84

The Lombardi Cancer Research Center, Georgetown University School of Medicine, Washington, DC 20007, USA.

A second adenomatous polyposis coli (APC)-like gene, APC2/APCL, was recently described and localized to chromosome 19. We have fine mapped APC2 to a small region of chromosome 19p13.3 containing markers D19S883 and WI-19632, a region commonly lost in a variety of cancers, particularly ovarian cancer. Read More

Physiol Rev 1999 Jul;79(3):661-82

Department of Biochemistry, University of Tennessee, Memphis, Tennessee, USA.

Intracellular Ca2+ is normally maintained at submicromolar levels but increases during many forms of cellular stimulation. This increased Ca2+ binds to receptor proteins such as calmodulin (CaM) and alters the cell's metabolism and physiology. Calcium-CaM binds to target proteins and alters their function in such a way as to transduce the Ca2+ signal. Read More

Biochemistry 1994 Nov;33(46):13571-80

Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139-4211.

The binding of the FK506/FKBP-12 complex to calcineurin (CN), its putative target for immunosuppression, involves recognition of solvent-exposed regions of the ligand as well as FKBP-12 residues near the active site. The R42K, H87V double mutation of FKBP-12 decreases the CN affinity of the complex by 550-fold [Aldape, R. A. Read More

J Biol Chem 1991 Apr;266(12):7950-6

Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

The domains on clathrin responsible for interaction with the plasma membrane-associated assembly protein AP-2 have been studied using a novel cage binding assay. AP-2 bound to pure clathrin cages but not to coat structures already containing AP that had been prepared by coassembly. Binding to preassembled cages also occurred in the presence of elevated Tris-HCl concentrations (greater than or equal to 200 mM) which block AP-2 interactions with free clathrin. Read More

Arch Surg 1989 Jun;124(6):673-5

Department of Surgery, University of Pittsburgh School of Medicine, PA.

Inflammatory aneurysms of the abdominal aorta (IAAAs) have distinctive clinical and physical characteristics that separate them from typical atherosclerotic aneurysms. They were identified in 19 (7.2%) of 265 patients undergoing abdominal aortic aneurysm repair. Read More

Proc Natl Acad Sci U S A 1978 Sep;75(9):4209-13

Exposure of adipocytes to antibodies to the insulin receptor results in a blockade of (125)I-labeled insulin binding, stimulation of glucose oxidation, and many more insulin-like effects. Allowing for differences in purity, antireceptor antibody is equipotent with insulin on a molar basis. Both the bivalent F(ab')(2) and monovalent Fab' fragments of the antireceptor antibody are fully active in inhibiting (125)I-labeled insulin binding. Read More