27 results match your criteria ivf ecs

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Clinical validity and utility of preconception expanded carrier screening for the management of reproductive genetic risk in IVF and general population.

Hum Reprod 2021 May 22. Epub 2021 May 22.

GeneraLife, Center for Reproductive Medicine, Clinica Valle Giulia, Rome, Italy.

Study Question: What is the clinical validity and utility of preconception Expanded Carrier Screening (ECS) application on the management of prospective parents?

Summary Answer: The high detection rate of at-risk couples (ARCs) and the high proportion opting for IVF/preimplantation genetic testing (PGT) treatment demonstrate the clinical utility of ECS in the preconception space in IVF and general population.

What Is Known Already: About 2-4% of couples are at risk of conceiving a child with an autosomal recessive or X-linked genetic disorder. In recent years, the increasing cost-effectiveness of genetic diagnostic techniques has allowed the creation of ECS panels for the simultaneous detection of multiple recessive disorders. Read More

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Cannabis alters epigenetic integrity and endocannabinoid signalling in the human follicular niche.

Hum Reprod 2021 May 5. Epub 2021 May 5.

CReATe Fertility Centre, Toronto, ON, Canada.

Study Question: Do phytocannabinoids (PCs) affect follicular endocannabinoid signalling and the epigenome in the surrounding granulosa cells (GCs)?

Summary Answer: Exposure to PCs increases the expression of endocannabinoid receptors and reduces DNA methylation enzyme expression and global DNA methylation in naïve GCs.

What Is Known Already: Cannabis plant derivatives, known as PCs, are used for medicinal and recreational purposes. The main PC, tetrahydrocannabinol (THC), is the third most commonly used substance by women of childbearing age, hence knowledge of the effect it has on reproduction is of utmost importance. Read More

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A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions.

Sci Rep 2021 Apr 12;11(1):7917. Epub 2021 Apr 12.

School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.

The isolation of sperm cells from background cell populations and debris is an essential step in all assisted reproductive technologies. Conventional techniques for sperm recovery from testicular sperm extractions stagnate at the sample processing stage, where it can take several hours to identify viable sperm from a background of collateral cells such as white bloods cells (WBCs), red blood cells (RBCs), epithelial cells (ECs) and in some cases cancer cells. Manual identification of sperm from contaminating cells and debris is a tedious and time-consuming operation that can be suitably addressed through inertial microfluidics. Read More

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Expanded carrier screening in Chinese patients seeking the help of assisted reproductive technology.

Mol Genet Genomic Med 2020 09 23;8(9):e1340. Epub 2020 Jun 23.

Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

Background: Expanded carrier screening (ECS) has emerged as an effective approach to identify at-risk couples (ARCs)-before they initiate attempts at reproduction-who possess a high probability of having a child affected by severe recessive diseases. The objective of this study was to evaluate the clinical utility of ECS in Chinese patients seeking the help of assisted reproductive technology (ART).

Methods: An ECS test, which covers 201 genes implicated in 135 recessive (autosomal or X-linked) diseases, was routinely offered to all ART patients in a single genetics and in vitro fertilization clinic. Read More

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September 2020

Interest in expanded carrier screening among individuals and couples in the general population: systematic review of the literature.

Hum Reprod Update 2020 04;26(3):335-355

Department of Public Health and Primary Care, Centre for Biomedical Ethics and Law, KU Leuven, 3000 Leuven, Belgium.

Background: Through carrier screening, prospective parents can acquire information about whether they have an increased risk of conceiving a child affected with an autosomal recessive or X-linked condition. Within the last decade, advances in genomic technologies have facilitated a shift from condition-directed carrier screening to expanded carrier screening (ECS). Following the introduction of ECS, several studies have been performed to gauge the interest in this new technology among individuals and couples in the general population. Read More

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Expanded genetic carrier screening in clinical practice: a current survey of patient impressions and attitudes.

J Assist Reprod Genet 2019 Apr 13;36(4):709-716. Epub 2019 Feb 13.

Department of Obstetrics and Gynecology, Boonshoft School of Medicine, Wright State University, 128 Apple Street, Suite 3800 Weber CHE, Dayton, OH, 45409, USA.

Purpose: Expanded genetic carrier screening (ECS) is an important part of gynecological practice and preconception planning. We evaluated the awareness and attitudes among women regarding ECS and factors that may influence decision-making in a family planning context.

Methods: A 32-question survey in an academic university practice was given to 521 women who were either currently pregnant (n = 108), undergoing gynecologic care who were considering future fertility (n = 308), and considering or receiving fertility treatment (n = 105). Read More

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Characterization of embryonic cells obtained from multifetal reduction.

Front Biosci (Elite Ed) 2019 01 1;11:79-88. Epub 2019 Jan 1.

Center of Excellence for Stem Cells and Regenerative Medicine (CESC), Zewail City of Science and Technology, Giza, Egypt,

The multifetal reduction (MFR) procedure is usually reserved for high-order multiple pregnancies, and aspirated tissues are typically discarded. In this study, cells obtained from MFR tissue (termed multifetal reduction embryonic cells (MFR-ECs)), were characterized by genotypic and phenotypic analyses and tested by injection under the kidney capsule of nude mice. MFR-ECs were highly proliferative in culture and showed a normal karyotype by microarray CGH. Read More

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January 2019

Lifestyle causes of male infertility.

Arab J Urol 2018 Mar 13;16(1):10-20. Epub 2018 Feb 13.

Discipline of Physiology, Faculty of Medicine, Sungai Buloh Campus, Universiti Teknologi MARA, Selangor, Malaysia.

Objective: To examine the potential effects of lifestyle factors on male reproductive health. Evidence of a global decline in human sperm quality over recent decades has been accumulating. Environmental, occupational, and modifiable lifestyle factors may contribute to this decline. Read More

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Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs.

Proc Natl Acad Sci U S A 2017 12 4;114(52):E11111-E11120. Epub 2017 Dec 4.

Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305;

Patient-specific pluripotent stem cells (PSCs) can be generated via nuclear reprogramming by transcription factors (i.e., induced pluripotent stem cells, iPSCs) or by somatic cell nuclear transfer (SCNT). Read More

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December 2017

Clinical Utility of Expanded Carrier Screening: Reproductive Behaviors of At-Risk Couples.

J Genet Couns 2018 06 27;27(3):616-625. Epub 2017 Sep 27.

Counsyl, 180 Kimball Way, South San Francisco, 94080, CA, USA.

Expanded carrier screening (ECS) analyzes dozens or hundreds of recessive genes to determine reproductive risk. Data on the clinical utility of screening conditions beyond professional guidelines are scarce. Individuals underwent ECS for up to 110 genes. Read More

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Changes in endothelial progenitor cell subsets in normal pregnancy compared with preeclampsia.

J Chin Med Assoc 2015 Jun 23;78(6):345-52. Epub 2015 May 23.

Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: The results of studies measuring the number of endothelial progenitor cells (EPCs) in normal pregnancies and in preeclampsia have been highly controversial or even contradictory because of cross-sectional designs and different methodologies enumerating three distinct subsets of EPCs: circulating angiogenic cells (CAC), colony-forming unit endothelial cell (CFU-ECs), and endothelial colony forming cells (ECFCs). To provide a clear explanation for these underlying controversies, we designed a prospective study to compare the number of all EPC subsets between three trimesters of normal gestation and a case-control study to compare these values as preeclampsia occurs with those from gestational age (GA) matched normal pregnancy.

Methods: Samples from peripheral blood of nine women were taken during their three consecutive trimesters of normal pregnancy, and from eight women with preeclampsia. Read More

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Intrafollicular Oocyte Transfer (IFOT) of Abattoir-Derived and In Vitro-Matured Oocytes Results in Viable Blastocysts and Birth of Healthy Calves.

Biol Reprod 2015 Jun 29;92(6):150. Epub 2015 Apr 29.

Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany

There are still major differences between in vitro production (IVP)-derived and in vivo-derived bovine blastocysts. Therefore, intrafollicular oocyte transfer (IFOT) was used in the present study to allow early embryonic development within the physiological oviductal environment, in order to avoid subsequent harmful effects of the in vitro culture environment. Using modified ovum pickup equipment, in vitro-matured oocytes were transferred into the preovulatory follicle of synchronized heifers (follicular recipients), enabling subsequent ovulation, in vivo fertilization, and in vivo development. Read More

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Clinical significance of sperm DNA damage in assisted reproduction outcome.

Hum Reprod 2010 Jul 6;25(7):1594-608. Epub 2010 May 6.

Centre for Public Health, Reproductive Medicine, Institute of Clinical Science, Queens University of Belfast, Grosvenor Road, Belfast BT12 6BJ, UK.

Background: Sperm DNA damage shows great promise as a biomarker of infertility. The study aim is to determine the usefulness of DNA fragmentation (DF), including modified bases (MB), to predict assisted reproduction treatment (ART) outcomes.

Methods: DF in 360 couples (230 IVF and 130 ICSI) was measured by the alkaline Comet assay in semen and in sperm following density gradient centrifugation (DGC) and compared with fertilization rate (FR), embryo cumulative scores (ECS(1)) for the total number of embryos/treatment, embryos transferred (ECS(2)), clinical pregnancy (CP) and spontaneous pregnancy loss. Read More

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The effect of long-term in vivo culture in bovine oviduct and uterus on the development and cryo-tolerance of in vitro produced bovine embryos.

Reprod Domest Anim 2010 Oct;45(5):832-7

Reproduction Centre - Wieselburg, University of Veterinary Medicine, Vienna, Austria.

The objective of this study was to compare the embryo production and quality carried out entirely in vitro or partly in vitro combined with short- vs long-term in vivo culture using the homologous cattle oviduct. The IVM oocytes were in vitro fertilized and cultured for 7 and 8 days (IVP-Group), or after IVF and 2-3 days of IVC, 4-8 cell stage embryos were endoscopically transferred into oviducts of synchronized heifers (In Vivo-Group) or IVM oocytes were co-incubated with spermatozoa for 3-4 h and transferred into the oviducts of synchronized heifers (GIFT-Group). Embryos of the In Vivo-Group and the GIFT-Group were recovered on day 7 from the oviducts and uterine horns. Read More

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October 2010

Tissue-specific effects of in vitro fertilization procedures on genomic cytosine methylation levels in overgrown and normal sized bovine fetuses.

Biol Reprod 2006 Jul 22;75(1):17-23. Epub 2006 Mar 22.

Institute of Molecular Animal Breeding and Biotechnology, Gene Center of the Ludwig-Maximilian University, D-81377 Munich, and Pediatric Endocrinology Section, University Hospital, Tuebingen, Germany.

Epigenetic perturbations are assumed to be responsible for phenotypic abnormalities of fetuses and offspring originating from in vitro embryo techniques. We studied 29 viable Day-80 bovine fetuses to assess the effects of two in vitro fertilization protocols (IVF1 and IVF2) on fetal phenotype and genomic cytosine methylation levels in liver, skeletal muscle, and brain. The IVF1 protocol employed 0. Read More

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Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification.

Theriogenology 2006 May 17;65(8):1551-62. Epub 2005 Oct 17.

Laboratorio de Producción in vitro de Embriones, Departamento de Producción Animal, INTA, CC 276 (7620) Balcarce, Argentina.

Unlabelled: The present study investigated the effect of estrous cow serum (ECS) during culture of bovine embryos on blastocyst development and survival after cryopreservation by slow freezing or vitrification. Embryos were derived from in vitro maturation (IVM) and in vitro fertilization (IVF) of abbatoir-derived oocytes. At Day 3, embryos were cultured in three different media: Charles Ronsenkrans medium + amino acids (CR1aa; without bovine serum albumin (BSA)) + 5% estrous cow serum (CR1-ECS), CR1aa + 3 mg/mL BSA (CR1-BSA) or CR1aa + 5% ECS + 3 mg/mL BSA (CR1-ECS-BSA). Read More

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Conversion of high-response gonadotropin intrauterine insemination cycles to in vitro fertilization results in excellent ongoing pregnancy rates.

Fertil Steril 2002 Apr;77(4):715-20

Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Objective: To determine whether conversion of gonadotropin/IUI cycles at high risk of high-order multiple pregnancies to IVF yields a pregnancy rate high enough to warrant the added intervention.

Design: Case-control study.

Setting: Brigham and Women's Hospital assisted reproductive technology program. Read More

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Efficient in vitro production of cat embryos in modified synthetic oviduct fluid medium: effects of season and ovarian status.

Biol Reprod 2001 Jul;65(1):9-13

Institute of Molecular Animal Breeding, Gene Center, Ludwig-Maximilian University, D-85764 Oberschleissheim, Germany.

The effects of season (January-March = I; April-June = II; July-September = III; October-December = IV) and ovarian status (freshly ovulated, follicular, luteal, intermediate, or inactive) on the efficiency of the in vitro production of domestic cat embryos were evaluated. Ovaries and testes from cats with access to daylight were collected at local veterinary clinics. A total of 6843 cumulus-oocyte complexes (COCs) were recovered from 363 pairs of ovaries, matured in TCM 199 supplemented with BSA and gonadotropins (IVM), fertilized with epididymal sperm in a medium based on Tyrode albumin lactate pyruvate (IVF), and cultured in synthetic oviduct fluid (SOF) medium supplemented with 10% estrous cow serum (ECS) and essential and nonessential amino acids. Read More

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Cytogenetic analysis of bovine parthenotes after spontaneous activation in vitro.

Theriogenology 1998 Mar;49(4):779-85

Agricultural University of Poznań, Department of Genetics and Animal Breeding, Poland.

We conducted a cytogenetic study of bovine parthenotes derived from oocytes matured and cultured in vitro. In vitro maturation was carried out by culturing follicular oocytes for 24 h in TCM199 supplemented with estrous cow serum (ECS) and hormones at 39 degrees C in 5% CO2. Matured oocytes were incubated for 20 h in sperm TALP without the addition of spermatozoa, after which they were cultured in maturation droplets for 48 to 72 h. Read More

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Evaluation of mitomycin-treated vero cells as a co-culture system for IVM/IVF-derived bovine embryos.

Theriogenology 1997 Aug;48(3):377-89

Center for Food and Animal Research, Agriculture and Agri-Food Canada Experimental Farm and Department of Physiology, University of Ottawa, Ottawa, Ontario, Canada.

Support of the in vitro development of IVM/IVF-derived bovine embryos by Vero cells was evaluated by comparing the following treatment groups: 1) proliferating (Unt-Vero) vs nonproliferating (Mit-Vero) cells; 2) supplementation of medium with estrous cow serum (ECS) vs bovine serum albumin (BSA); 3) Mit-Vero cells vs bovine oviduct epithelial cells (BOECs); and 4) addition of leukemia inhibitory factor (LIF) to Mit-Vero cell co-cultures at Day 1 vs Day 4. Mit-Vero cells stimulated higher rates of blastocysts (Day 7, 40 vs 27%) and hatched blastocyst (Day 10, 38 vs 12%) formation than Unt-Vero cells. These rates were comparable to those obtained with BOECs; blastocyst hatching was slightly higher following co-culture with Mit-Vero cells (36%) than BOECs (29%). Read More

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Cytogenetic evaluation of in vitro matured bovine oocytes collected from ovaries of individual donors.

Theriogenology 1996 Mar;45(4):865-72

Department of Genetics and Animal Breeding, Agricultural University of Poznań, Poland.

Oocytes were collected after slaughter by aspiration from pairs of ovaries of individual donors. A total of 656 oocytes was selected for IVM from 74 pairs of ovaries (8.9 oocytes per pair, ranging between 1 and 25). Read More

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In vitro maturation and fertilization of equine oocytes recovered during the breeding season.

Theriogenology 1996 Feb;45(3):547-60

Institute of Biology of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Bari, Italy.

The aim of this study was to develope an efficient and reproducible procedure for in vitro maturation (IVM) and fertilization (IVF) in the horse. Cumulus-oocyte complexes (COCs) recovered from the ovaries of mares slaughtered during the breeding season were morphologically evaluated, and those showing a compact cumulus and homogeneously appearing cytoplasm were selected for culture. Effects on the maturation of estrous mare serum (EMS) versus estrous cow serum (ECS) as medium supplement were also evaluated (Experiment 1). Read More

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February 1996

The effect of serum on in vitro maturation, in vitro fertilization and steroidogenesis of bovine oocytes co-cultured with granulosa cells.

Braz J Med Biol Res 1995 Feb;28(2):213-7

Departamento de Apoio, Produção e Saúde Animal, Universidade Estadual Paulista, Araçatuba, SP, Brasil.

The influence of fetal calf serum alone (FCS) or associated with proestrous (FCS+PCS), estrous (FCS+ECS) or metaestrous (FCS+MCS) cow serum added to the culture medium and of the steroids produced by co-cultured granulosa cells were evaluated in terms of the in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes. Supplementation of the medium with FCS+ECS and FCS+MCS resulted in higher proportions of oocytes that reached metaphase II (96.0% and 93. Read More

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February 1995

Evaluation of culture systems containing bovine oviduct epithelial cells or granulosa cells to mature and maintain the developmental competence of bovine oocytes in vitro.

Theriogenology 1994 Aug;42(2):261-72

Centre for Food and Animal Research, Agriculture and Agrifood Canada Ottawa, Ontario, Canada K1A OC6.

The effects of estrous cow serum (ECS), bovine oviduct epithelial cells (BOEC), and bovine granulosa cells (GC) on in vitro maturation (IVM) of immature oocyte-cumulus complexes (OCCs) were evaluated. Selected OCCs were cultured for 24 to 26 h in microdroplets of culture medium (CM; TCM 199 + 25 mM HEPES + 100 mug gentamicin sulfate/ml) or in CM medium supplemented or conditioned with 20% ECS, BOEC +/- 20% ECS or GC + 20% ECS. Supplemented media were incubated for 2 h before addition of OCCs, whereas media were conditioned by incubation with 20% ECS or BOEC +/- 20% ECS for 6 d, or with 20% ECS +/- GC for 24 or 48 h before addition of OCCs. Read More

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Human follicular fluid maturity and endothelial cell chemotaxis.

Hum Reprod 1994 Jul;9(7):1226-30

Monash University Department of Obstetrics and Gynaecology, Clayton, Victoria, Australia.

Follicular fluid of varying maturity was collected from the largest Graafian follicle of 23 ovulatory patients during laparoscopy (five blood-stained samples were discarded) and from five patients undergoing oocyte collection for assisted reproduction. The endothelial cell (EC) chemotactic potentials of the samples were tested with bovine aortic ECs in modified Boyden Chambers and an EC density score was determined for each sample. Intra-assay coefficients of variation (CV) varied from 2. Read More

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A serum-free, cell-free culture system for development of bovine one-cell embryos up to blastocyst stage with improved viability.

Theriogenology 1994 ;41(5):1033-43

Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Swedish University of Agricultural Sciences, Box 7039, S-750 07 Uppsala, Sweden.

With the aim of developing a serum-free, cell-free culture system for embryo development, in vitro-matured (IVM) and -fertilized (IVF) bovine oocytes were cultured in TCM 199 with the following supplements: 1) BSA alone (10 mg/ml); 2) BSA with ITS (5 mug/ml insulin, 5 mug/ml transferrin and 5 ng/ml selenium; BSAITS medium); 3) estrous cow serum alone (ECS; 10%); or 4) ECS with BOEC (bovine oviduct epithelial cells) (Experiment 1). In Experiment 2, embryos were cultured in BSAITS medium with or without feeding with fresh medium on Day 4 (day of insemination = Day 0). Embryos were evaluated on Day 2 for first cleavage, on Day 7 for morulae and blastocysts, and on Day 8 for blastocysts. Read More

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October 2012

Culture medium and protein supplementation influence in vitro fertilization and embryo development in the domestic cat.

J Exp Zool 1991 Mar;257(3):350-9

National Zoological Park, Smithsonian Institution, Washington, District of Columbia 20008.

The influence of culture medium and protein supplements on in vitro fertilization (IVF) and morula-to-blastocyst development in culture was examined in the domestic cat. In Study I, follicular oocytes were fertilized and cultured in 1) modified Krebs Ringer bicarbonate (mKRB); 2) modified Tyrode's solution (TALP) without phosphate or glucose; or 3) Ham's F10. All media contained bovine serum albumin (BSA). Read More

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