159,564 results match your criteria isolation three


Sociodemographic Characteristics and Physical Activity in Patients with COPD: A 3-Month Cohort Study.

COPD 2021 May 10:1-13. Epub 2021 May 10.

Department of Medicine, University of Washington, Seattle, WA, USA.

Decreased physical activity (PA) is associated with morbidity and mortality in COPD patients. In this secondary analysis of data from a 12-week longitudinal study, we describe factors associated with PA in COPD. Participants completed the Physical Activity Checklist (PAC) daily for a 7- to 8-day period. Read More

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PthAW1, a transcription activator-like effector of Xanthomonas citri subsp. citri, promotes host specific immune responses.

Mol Plant Microbe Interact 2021 May 10. Epub 2021 May 10.

University of Florida, Citrus Research and Education Center/Department of Microbiology and Cell Science, 700 Experiment Station Road, Lake Alfred, Florida, United States, 33850;

Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most destructive diseases in citrus. XccA causes canker disease in most commercial citrus varieties, whereas XccAW, which is genetically similar to XccA, infects only lime and alemow. Read More

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Cross-Cultural Comparison of Nonopioid and Multimodal Analgesic Prescribing in Orthopaedic Trauma.

J Am Acad Orthop Surg Glob Res Rev 2020 May;4(5):e2000051

From the Harvard Medical School (Mr. Young, Dr. Bhashyam, Dr. Dyer); the Harvard Combined Orthopaedic Residency Program (Dr. Bhashyam, Dr. Dyer); Boston, MA; the Department of Orthopaedic Surgery (Dr. Parisien), University of Pennsylvania; Philadelphia, PA; the Department of Trauma Surgery (Dr. Van der Vliet), University Medical Center Utrecht; Utrecht, the Netherlands; the Department of Orthopaedics, Nemours (Dr. Qudsi)/A.I. duPont Hospital for Children; Wilmington, DE; and the Department of Orthopaedic Surgery (Dr. Fils, Dr. Dyer), Brigham and Women's Hospital, Boston, MA.

Background: After musculoskeletal injury, US providers prescribe opioids more frequently and at higher dosages than prescribers in the Netherlands and Haiti; however, the extent of variation in nonopioid analgesic prescribing is unknown. The aim of our study was to evaluate how nonopioid prescribing by orthopaedic residents varies by geographic context.

Methods: Orthopaedic residents in three countries in which residents are the primary prescribers of postoperative analgesia in academic medical centers (Haiti, the Netherlands, and the United States) responded to surveys using vignette-based musculoskeletal trauma case scenarios. Read More

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Mitochondrial damage produced by phytotoxic chromenone and chromanone derivatives from endophytic fungus Daldinia eschscholtzii strain GsE13.

Appl Microbiol Biotechnol 2021 May 10. Epub 2021 May 10.

Instituto de Química, Departamento de Productos Naturales, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Delegación Coyoacán, 04510, Mexico City, Mexico.

Bioassay-guided fractionation of the organic extracts of the endophyte Daldinia eschscholtzii strain GsE13 led to the isolation of several phytotoxic compounds, including two chromenone and two chromanone derivatives: 5-hydroxy-8-methoxy-2-methyl-4H-chromen-4-one, 1; 5-hydroxy-2-methyl-4H-chromen-4-one, 2; 5-methoxy-2-methyl-chroman-4-one, 3; and 5-methoxy-2-methyl-chroman-4-ol, 4; as well as other aromatic compounds: 4,8-dihydroxy-1-tetralone, 5; 1,8-dimethoxynaphthalene, 6; and 4,9-dihydroxy-1,2,11,12-tetrahydroperyl-ene-3,10-quinone, 7. Compounds 1, 4, and 7 were isolated for the first time from D. eschscholtzii. Read More

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A Series of Surgical Infections and Review of the Literature.

Surg Infect (Larchmt) 2021 May 7. Epub 2021 May 7.

University of Maryland Shore Regional at Easton, Easton, Maryland, USA.

(HPI) is a rare and underreported pathogen. causes respiratory, soft tissue, and central nervous system (CNS) infections, and endocarditis. Little data on HPI surgical infections are available, especially for intra-abdominal infections (IAI). Read More

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First report of Corynespora cassicola causing black spot on Sarcandra glabra in China.

Plant Dis 2021 May 10. Epub 2021 May 10.

Guangxi Botanical Garden of Medicinal Plants, 248907, Nanning, Guangxi, China;

Sarcandra glabra is an important Chinese medicinal plant, which was widely cultivated under forest in south China. Guangxi province is the main producing areas of this herb. In June 2019, a serious leaf disease was found causing severe defoliation in the S. Read More

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First Report of Pectobacterium brasiliense causing soft rot on mizuna (Brassica rapa var. japonica) in the United States.

Plant Dis 2021 May 10. Epub 2021 May 10.

University of Hawai'i at Manoa, 3949, Plant and Environmental Protection Sciences, Honolulu, Hawaii, United States;

Mizuna (Brassica rapa var. japonica), a member of family Brassicaceae, is a leafy vegetable having phenolic and other compounds beneficial to human health, such as natural antioxidants (Khanam et al. 2012). Read More

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COVID-19 Awareness and Preparedness of Minnesota and Wisconsin Dairy Farms.

J Agromedicine 2021 May 9. Epub 2021 May 9.

University of Minnesota School of Public Health, Minneapolis, MN, USA.

: Investigate biosecurity principles on Minnesota and Wisconsin dairy farms in response to COVID-19 and the effects of the pandemic on the dairy industry.: Dairy farms that had participated in previous and ongoing projects with the National Farm Medicine Center (NFMC), Migrant Clinicians Network (MCN), and Upper Midwest Agricultural Safety and Health Center (UMASH) were asked to participate in a 17-question survey by phone or email. Three additional farms were recruited via a press release published in agricultural newsletters. Read More

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Isolation and identification of guaiacol producing Alicyclobacillus fastidiosus strains from orchards in Poland.

Acta Biochim Pol 2021 May 10. Epub 2021 May 10.

Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Warsaw, Poland.

The genus Alicyclobacillus comprises a group of Gram-positive, thermo-acidophilic bacteria that are capable of producing highly resistant endospores during unfavorable environmental conditions. The members of this genus inhabit natural environments, including hot springs and soils. The main reason behind the spoilage of final commercial fruit products by Alicyclobacillus is the contamination of fruits with soil at the time of harvesting. Read More

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Old age genetically confirmed frontotemporal lobar degeneration with TDP-43 has limbic predominant TDP-43 deposition.

Neuropathol Appl Neurobiol 2021 May 9. Epub 2021 May 9.

Department of Neurology, Mayo Clinic, 200 1stStreet SW, Rochester, MN, 55905, USA.

Aims: To assess the burden of transactive response DNA-binding protein of 43kDa (TDP-43) inclusions in a unique cohort of old-age patients with genetic-frontotemporal lobar degeneration (gFTLD-TDP) and compare these patients with sporadic old-age individuals with TDP-43, either in the presence of Alzheimer's disease (AD-TDP) or in isolation (pure-TDP).

Methods: The brain bank at Mayo Clinic-Jacksonville was searched for cases ≥75 years old at death with TDP-43 extending into middle frontal cortex. Cases were split into the following groups: 1) gFTLD-TDP (n=15) with progranulin (GRN)/C9ORF72 mutations; 2) AD-TDP (n=10)-cases with median Braak neurofibrillary tangle (NFT) stage VI, Thal phase V; 3) pure-TDP (n=10)-cases with median Braak NFT stage I, Thal phase I. Read More

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Case Report: Stepwise Anti-Inflammatory and Anti-SARS-CoV-2 Effects Following Convalescent Plasma Therapy With Full Clinical Recovery.

Front Immunol 2021 21;12:613502. Epub 2021 Apr 21.

Interregional Blood Transfusion SRC, Epalinges, Switzerland.

In these times of COVID-19 pandemic, concern has been raised about the potential effects of SARS-CoV-2 infection on immunocompromised patients, particularly on those receiving B-cell depleting agents and having therefore a severely depressed humoral response. Convalescent plasma can be a therapeutic option for these patients. Understanding the underlying mechanisms of convalescent plasma is crucial to optimize such therapeutic approach. Read More

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Genomics of Environmental : Engaging Students in the Microbiology and Bioinformatics of Foodborne Pathogens.

Front Microbiol 2021 22;12:592422. Epub 2021 Apr 22.

Department of Biology, James Madison University, Harrisonburg, VA, United States.

We have developed and implemented an undergraduate microbiology course in which students isolate, characterize, and perform whole genome assembly and analysis of from stream sediments and poultry litter. In the development of the course and over three semesters, successive teams of undergraduate students collected field samples and performed enrichment and isolation techniques specific for the detection of . Eighty-eight strains were confirmed using standard microbiological methods and PCR of the gene. Read More

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Isolation and HPLC assisted quantification of two iridoid glycoside compounds and molecular DNA fingerprinting in critically endangered medicinal Royle ex Benth: implications for conservation.

Physiol Mol Biol Plants 2021 Apr 26;27(4):727-746. Epub 2021 Mar 26.

Department of Botany, University of Delhi, 110007 New Delhi, Delhi India.

is a medicinally important, high altitude perennial herb, endemic to the Himalayas. It possesses strong hepato-protective bioactivity that is contributed by two iridoid picroside compounds viz Picroside-I (P-I) and Picroside-II (P-II). Commercially, many based hepato-stimulatory Ayurvedic drug brands that use different proportions of P-I and P-II are available in the market. Read More

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Fabrication of PNIPAm-based thermoresponsive hydrogel microwell arrays for tumor spheroid formation.

Mater Sci Eng C Mater Biol Appl 2021 Jun 14;125:112100. Epub 2021 Apr 14.

Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA. Electronic address:

Complex three-dimensional (3D) cell cultures are being increasingly implemented in biomedical research as they provide important insights into complex cancer biology, and cell-cell and cell-matrix interactions in the tumor microenvironment. However, most methods used today for 3D cell culture are limited by high cost, the need for specialized skills, low throughput and the use of unnatural culture environments. We report the development of a unique biomimetic hydrogel microwell array platform for the generation and stress-free isolation of cancer spheroids. Read More

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Extensive pollen-mediated gene flow across intensively managed landscapes in an insect-pollinated shrub native to semiarid habitats.

Mol Ecol 2021 May 9. Epub 2021 May 9.

Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, E-23071, Jaén, Spain.

Our knowledge of the impact of landscape fragmentation on gene flow patterns is mainly drawn from tropical and temperate ecosystems, where landscape features, such as the distance of a tree to the forest edge, drive connectivity and mating patterns. Yet, the structure of arid and semiarid plant communities - with open canopies and a scattered distribution of trees - differs greatly from those that are well-characterized in the literature. As a result, we ignore whether the documented consequences of landscape fragmentation on plant mating and gene flow patterns also hold for native plant communities in arid and semiarid regions. Read More

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Use of virtual reality in the inpatient rehabilitation of COVID-19 patients.

Gen Hosp Psychiatry 2021 Apr 29;71:76-81. Epub 2021 Apr 29.

Department of Medicine, Weill Cornell Medicine, New York, NY; Division of Hospital Medicine, Ascension Saint Thomas Hospital West, Nashville, TN.

Introduction: Use of virtual reality (VR) in healthcare has expanded in recent years. The challenges faced by patients with prolonged COVID-19-related hospitalizations - social isolation, disability, neurologic sequelae, adjustment-related anxiety, depression, and stress - may be mitigated by the novel use of VR as one modality of a comprehensive rehabilitation plan. This descriptive study aimed to understand patient satisfaction and perceived benefit of virtual reality on a COVID-19 recovery unit, as well as the logistical and operational feasibility of providing VR content for patients and staff. Read More

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"Accidents waiting to happen" - insights from a simple model on the emergence of infectious agents in new hosts.

Transbound Emerg Dis 2021 May 8. Epub 2021 May 8.

Biological Interactions, Universidade Federal do Paraná, Curitiba, PR, 81531-980, Brazil.

This study evaluates through modeling the possible individual and combined effect of three populational parameters of pathogens (reproduction rate; rate of novelty emergence; and propagule size) on the colonization of new host species - putatively the most fundamental process leading to the emergence of new infectious diseases. The results are analyzed under the theoretical framework of the Stockholm Paradigm using IBM simulations to better understand the evolutionary dynamics of the pathogen population and the possible role of Ecological Fitting. The simulations suggest that all three parameters positively influence the success of colonization of new hosts by a novel parasite population but contrary to the prevailing belief, the rate of novelty emergence (e. Read More

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A review of the impact of hearing interventions on social isolation and loneliness in older people with hearing loss.

Eur Arch Otorhinolaryngol 2021 May 7. Epub 2021 May 7.

Department of ENT, Tameside and Glossop Integrated Care NHS Foundation Trust, Ward 29 Charlesworth Building, Fountain Street, Ashton-under-Lyne, OL6 9RW, UK.

Purpose: Hearing loss affects many older people and is associated with social isolation and loneliness. The impact of hearing interventions however has not been established. The objective of this review is to determine the impact of hearing interventions in older people with hearing loss on social isolation and loneliness. Read More

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The use of dual oxygen concentrator system for mechanical ventilation during COVID-19 pandemic in Sabah, Malaysia.

Int J Emerg Med 2021 May 7;14(1):30. Epub 2021 May 7.

Department of Community Emergency Health and Paramedic Practice, Faculty of Medicine, Nursing and Health Sciences, Monash University, Frankston, Victoria, Australia.

Sabah in Malaysian Borneo is among the Malaysian states which reported a high number of detected COVID-19 cases during the current pandemic. Due to geographical challenges and limited resources, clinicians developed novel strategies for managing patients. The use of a dual oxygen concentrator system for mechanical ventilation is one of the innovations developed by retrieval team members from the Emergency Department (ED) of the Sabah Women and Children's Hospital. Read More

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Long-term shedding of viable SARS-CoV-2 in kidney transplant recipients with COVID-19.

Am J Transplant 2021 May 7. Epub 2021 May 7.

Department of Virology, Strasbourg University Hospital, Strasbourg, France.

The exact duration of viable SARS-CoV-2 shedding in kidney transplant recipients (KTRs) remains unclear. Here, we retrospectively investigated this issue using cell cultures of SARS-CoV-2 RT-PCR-positive nasopharyngeal samples (n = 40) obtained from 16 KTRs with symptomatic COVID-19 up to 39 days from symptom onset. A length of viable SARS-CoV-2 shedding >3 weeks from the onset of symptoms was identified in four KTRs (25%). Read More

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Cutibacterium modestum and "Propionibacterium humerusii" represent the same species that is commonly misidentified as Cutibacterium acnes.

Antonie Van Leeuwenhoek 2021 May 7. Epub 2021 May 7.

Division of Clinical Bacteriology and Mycology, University Hospital Basel, University of Basel, Petersgraben 4, 4031, Basel, Switzerland.

Cutibacterium spp. play an increasing role in soft tissue and implant-associated infections. We isolated a novel Cutibacterium spp. Read More

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[Position paper of the Italian Society of Cardiology (SIC) on COVID-19 vaccine priority in patients with cardiovascular diseases].

G Ital Cardiol (Rome) 2021 May;22(5):363-375

Società Italiana di Cardiologia, Roma - Mediterranea Cardiocentro, Napoli - Dipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli "Federico II", Napoli.

In over a year, the COVID-19 pandemic caused 2.69 million deaths and 122 million infections. Social isolation and distancing measures have been the only prevention available for months. Read More

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[50 years of electrophysiology and cardiac pacing].

G Ital Cardiol (Rome) 2021 May;22(5):341-351

U.O.C. Cardiologia, Ospedale G.B. Grassi, Ostia (RM).

During the last 50 years a continuous improvement was observed in the field of cardiac pacing and electrophysiology as for as both technological and clinical aspects were concerned. We moved from the first recording of the His bundle electrogram to the identification of the various mechanisms and sites of supraventricular and ventricular tachyarrhythmias, to three-dimensional mapping and ablation of different reentry circuits and eventually to pulmonary vein isolation for the treatment of atrial fibrillation. As far as cardiac pacing is concerned, we moved from single chamber to dual chamber pacing, to ventricular pacing and implantable automatic defibrillators provided by sophisticated diagnostic and therapeutic algorithms and by remote control. Read More

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Verrucous Carcinoma of the Esophagus Is A Genetically Distinct Subtype of Esophageal Squamous Cell Carcinoma.

Histopathology 2021 May 7. Epub 2021 May 7.

Department of Pathology.

Aims: Esophageal verrucous carcinoma (VSCC) is a rare and morphologically distinct type of esophageal squamous cell carcinoma (SCC). Diagnosing VSCC on biopsy material is challenging given the lack of significant atypia and the presence of keratinizing epithelium and exophytic growth. The molecular pathogenesis of VSCC remains unclear. Read More

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A new oleanane-skeleton triterpene isolated from .

Nat Prod Res 2021 May 7:1-7. Epub 2021 May 7.

Department of Chemical Technology, Ho Chi Minh University of Technology and Education, Ho Chi Minh City, Vietnam.

Extensive fractionation of -hexane extract from the dried powdered-trunks of Pierre ex A.Froehner (Rubiaceae) led to the isolation of a new oleanane-skeleton triterpene, coffecanolic acid (), along with three known analogues sumaresinolic acid (), oleanolic acid (), and 3--acetyloleanolic acid (). The chemical structures were elucidated using FT-IR, 1D and 2D NMR and HR-ESI-MS data analysis. Read More

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The influence of semantic context on false memories.

Mem Cognit 2021 May 6. Epub 2021 May 6.

Department of Psychology, University of Texas at Arlington, Arlington, TX, 76019, USA.

Although false memories have largely been examined with the Deese-Roediger-McDermott (DRM) paradigm, little research has focused on the semantic context in which associates are encoded. Across three experiments, we varied semantic context during a sentence-processing task with DRM associates embedded within sentences. More meaningful sentences resulted in greater memory errors (Experiment 1). Read More

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Molecular Typing of Community-acquired Methicillin-Resistant Isolated from 2- to 6-year old Children by Staphylococcal Protein A and Typing in Isfahan, Iran.

Adv Biomed Res 2021 27;10. Epub 2021 Jan 27.

Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Methicillin-resistant (MRSA) has become a considerable public health concern in the entire world due to the rapid spread of this bacterium in human community; also the epidemiology of MRSA has changed, as the isolation of MRSA strains from healthy and non-healthy patients. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of community-acquired (CA)-MRSA nasal carriage in the Iranian samples.

Materials And Methods: A total of 25 CA-MRSA were isolated from the anterior nares of 410 healthy preschool children. Read More

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January 2021

Exploring Biocontrol Agents From Microbial Keystone Taxa Associated to Suppressive Soil: A New Attempt for a Biocontrol Strategy.

Front Plant Sci 2021 19;12:655673. Epub 2021 Mar 19.

Pest Integrated Management Key Laboratory of China Tobacco, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.

Recent studies have observed differing microbiomes between disease-suppressive and disease-conducive soils. However, it remains unclear whether the microbial keystone taxa in suppressive soil are critical for the suppression of diseases. Bacterial wilt is a common soil-borne disease caused by that affects tobacco plants. Read More

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[Postoperative Inguinal Hernia after Robot-Assisted Radical Prostatectomy with Prevention Technique Using Spermatic Cord Isolation].

Hinyokika Kiyo 2021 Mar;67(3):91-95

The Department of Urology, Hiroshima City Asa Hospital.

We retrospectivelyevaluated postoperative inguinal hernias (PIHs) after robot-assisted radical prostatectomy(RARP) with a technique for preventing hernias byspermatic cord isolation. Among the RARPs performed from 2016 to 2018, 191 cases were evaluated 12 or more months after surgery. In all the cases, the peritoneum was isolated from the spermatic cord by5 cm or more as a hernia prevention technique during RARP. Read More

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Assessment of captive rearing conditions on loggerhead hatchlings: Effect of handling frequency and stocking density.

J Exp Zool A Ecol Integr Physiol 2021 May 6. Epub 2021 May 6.

ECOAQUA UI, University of Las Palmas de Gran Canaria. Parque Científico Tecnológico Marino, Las Palmas, Spain.

Frequently, stranded sea turtles require rehabilitation under controlled conditions. Currently, few publications have described the conditions under which rehabilitation is to take place, particularly with respect to the hatchling life stage. To address this paucity of data, we conducted some experiments to assist rehabilitating facilities assess their handling of hatchlings. Read More

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