215 results match your criteria isolates histoplasma

Equine Histoplasmosis in Ethiopia: Phylogenetic Analysis by Sequencing of the Internal Transcribed Spacer Region of rRNA Genes.

Front Cell Infect Microbiol 2022 8;12:789157. Epub 2022 Jul 8.

College of Veterinary Medicine and Agriculture, Addis Ababa University, Debre Zeit, Ethiopia.

Equine histoplasmosis commonly known as epizootic lymphangitis (EL) is a neglected granulomatous disease of equine that is endemic to Ethiopia. It is caused by variety , a dimorphic fungus that is closely related to variety c The objective of this study was to undertake a phylogenetic analysis of isolated from EL cases of horses in central Ethiopia and evaluate their relationship with isolates in other countries and/or clades using the internal transcribed spacer (ITS) region of rRNA genes. Clinical and mycological examinations, DNA extraction, polymerase chain reaction (PCR), Sanger sequencing, and phylogenetic analysis were used for undertaking this study. Read More

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A Langmuir-Blodgett Study of the Interaction between Amphotericin B and Lipids of .

Membranes (Basel) 2022 Apr 29;12(5). Epub 2022 Apr 29.

Grupo Interdisciplinario de Estudios Moleculares, Institute of Chemistry, Faculty of Exact and Natural Sciences, Universidad de Antioquia, Medellín 050010, Colombia.

is a dimorphic, thermal, and nutritional fungus. In the environment and at an average temperature of 28 °C, it develops as a mold that is composed of infecting particles. Once in the host or in cultures at 37 °C, it undergoes a transition into the parasitic form. Read More

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Histoplasmosis acquired in Alberta, Canada: an epidemiological and genomic study.

Lancet Microbe 2021 05 3;2(5):e191-e197. Epub 2021 Mar 3.

Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, AB, Canada.

Background: The classic geographical range of histoplasmosis in North America primarily includes the states and provinces adjacent to the Ohio, Mississippi, and St Lawrence riverways. Although Alberta, Canada is not typically considered a region of risk for histoplasmosis, cases with suspected local acquisition have been reported. We aimed to investigate the epidemiology and geographical distribution of cases of histoplasmosis in Alberta to assess evidence for local acquisition of infections, using genomic analysis for corroboration. Read More

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Considerations about the Geographic Distribution of Species.

Appl Environ Microbiol 2022 04 9;88(7):e0201021. Epub 2022 Mar 9.

Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico.

Histoplasmosis is a mycotic infection principally affecting pulmonary tissue; sometimes, histoplasmosis can progress into a systemic disease. This infection involves immunocompetent and immunosuppressed human and other mammalian hosts, depending on particular circumstances. Histoplasmosis infection has been documented worldwide. Read More

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Chromosome-Level Genome Assembly of a Human Fungal Pathogen Reveals Synteny among Geographically Distinct Species.

mBio 2022 Jan 4:e0257421. Epub 2022 Jan 4.

Department of Infectious Diseases, J. Craig Venter Institute, La Jolla, California, USA.

Histoplasma capsulatum, a dimorphic fungal pathogen, is the most common cause of fungal respiratory infections in immunocompetent hosts. is endemic in the Ohio and Mississippi River Valleys in the United States and is also distributed worldwide. Previous studies have revealed at least eight clades, each specific to a geographic location: North American classes 1 and 2 (NAm 1 and NAm 2), Latin American groups A and B (LAm A and LAm B), Eurasian, Netherlands, Australian and African, and an additional distinct lineage (H81) comprised of Panamanian isolates. Read More

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January 2022

An Indian lineage of Histoplasma with strong signatures of differentiation and selection.

Fungal Genet Biol 2022 01 21;158:103654. Epub 2021 Dec 21.

Department of Biology, University of North Carolina, Chapel Hill, NC, United States. Electronic address:

Histoplasma, a genus of dimorphic fungi, is the etiological agent of histoplasmosis, a pulmonary disease widespread across the globe. Whole genome sequencing has revealed that the genus harbors a previously unrecognized diversity of cryptic species. To date, studies have focused on Histoplasma isolates collected in the Americas with little knowledge of the genomic variation from other localities. Read More

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January 2022

Disseminated histoplasmosis in Peruvian non-HIV patients: an underrecognized endemic disease.

J Mycol Med 2021 Dec 28;31(4):101177. Epub 2021 Jul 28.

Departamento de Enfermedades Infecciosas, Tropicales y Dermatoloógicas, Hospital Cayetano Heredia, Lima, Perú; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.

Background: Histoplasmosis is a worldwide mycosis with endemic areas throughout Latin America. Disseminated histoplasmosis (DH) has been described in Peru, but there are few reports about its clinical presentation in non-HIV patients.

Methods: Case series was done with review of clinical and laboratory charts from Hospital Cayetano Heredia and Instituto de Medicina Tropical Alexander von Humboldt. Read More

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December 2021

New Diagnostic Assays Designed via Whole Genome Comparisons.

J Fungi (Basel) 2021 Jul 9;7(7). Epub 2021 Jul 9.

Cellular and Molecular Biology Unit, Corporación para Investigaciones Biológicas (CIB), Medellín 05534, Colombia.

Histoplasmosis is a systemic fungal disease caused by the pathogen spp. that results in significant morbidity and mortality in persons with HIV/AIDS and can also affect immunocompetent individuals. Although some PCR and antigen-detection assays have been developed, conventional diagnosis has largely relied on culture, which can take weeks. Read More

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Isolated from Bats Captured in Mexico Form a Sister Group to North American Class 2 Clade.

J Fungi (Basel) 2021 Jun 30;7(7). Epub 2021 Jun 30.

Unidad de Micología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, Mexico.

is a dimorphic fungus associated with respiratory and systemic infections in mammalian hosts that have inhaled infective mycelial propagules. A phylogenetic reconstruction of this pathogen, using partial sequences of , , , and protein-coding genes, proposed that has at least 11 phylogenetic species, highlighting a clade (BAC1) comprising three isolates from infected bats captured in Mexico. Here, relationships for each individual locus and the concatenated coding regions of these genes were inferred using parsimony, maximum likelihood, and Bayesian inference methods. Read More

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Evaluation of a New Histoplasma spp. Quantitative RT-PCR Assay.

J Mol Diagn 2021 06 9;23(6):698-709. Epub 2021 Mar 9.

Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Assistance Publique-Hôpitaux de Paris, Paris, France; Molecular Mycology Unit, CNRS UMR2000, Institut Pasteur, Paris, France; National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris, France; UFR de Médecine, Hôpital Necker Enfants malades, Assistance Publique-Hôpitaux de Paris, IHU Imagine, Paris Descartes University, Université de Paris, Paris, France.

Laboratory diagnosis of histoplasmosis is based on various methods, including microscopy, culture, antigen, and DNA detection of Histoplasma capsulatum var. capsulatum or Histoplasma capsulatum var. duboisii. Read More

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The global epidemiology of emerging species in recent years.

Stud Mycol 2020 Sep 2;97:100095. Epub 2020 Mar 2.

Laboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, 04023-062, Brazil.

Histoplasmosis is a serious infectious disease in humans caused by spp. (Onygenales), whose natural reservoirs are thought to be soil enriched with bird and bat guano. The true global burden of histoplasmosis is underestimated and frequently the pulmonary manifestations are misdiagnosed as tuberculosis. Read More

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September 2020

Cross-reactivity of a Histoplasma capsulatum antigen enzyme immunoassay in urine specimens from persons with emergomycosis in South Africa.

Med Mycol 2021 Jul;59(7):672-682

National Institute for Communicable Diseases (Centre for Healthcare-Associated Infections, Antimicrobial Resistance and Mycoses), a Division of the National Health Laboratory Service, Johannesburg, South Africa.

Histoplasma antigen detection in urine is a rapid diagnostic method for disseminated histoplasmosis, although cross-reactivity has been reported in specimens from patients with other thermally dimorphic fungal infections. We tested urine specimens, from persons with suspected invasive fungal infections, using a commercial monoclonal antibody Histoplasma enzyme immunoassay (EIA) at a South African national mycology reference laboratory from August 2014 through December 2018. Corresponding fungal culture and histopathology results were obtained from an electronic laboratory information system. Read More

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Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus .

J Venom Anim Toxins Incl Trop Dis 2020 Oct 28;26:e20200023. Epub 2020 Oct 28.

Department of Tropical Disease and Imaging Diagnosis, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.

Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or non-infectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. Read More

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October 2020

In Vitro Evaluation of the Effects of Selected Plants on the Growth of the Mycelial Form of Histoplasma capsulatum Variety farciminosum in Ethiopia.

J Equine Vet Sci 2020 08 30;91:103139. Epub 2020 May 30.

Department of Clinical Studies, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia. Electronic address:

Epizootic lymphangitis is prevalent in equines in Ethiopia, causing remarkable economic and welfare impacts but often neglected. Lack of effective treatment contributed to its continued occurrence, and hence, search for an effective treatment should be considered a priority area to minimize its impacts. Previous ethnobotanical studies have reported that Curcuma longa, Phytolacca dodecandra, and Datura stramonium were used to treat cutaneous fungal infections and reduce their incidence. Read More

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Histoplasma capsulatum chemotypes I and II induce IL-8 secretion in lung epithelial cells in distinct manners.

Med Mycol 2020 Nov;58(8):1169-1177

Department of Microbiology, Immunology, and Parasitology, Escola Paulista de Medicina - Universidade Federal de São Paulo, São Paulo - SP, Brazil.

The cell wall is one of the most important structures of pathogenic fungi, enabling initial interaction with the host and consequent modulation of immunological responses. Over the years, some researchers have shown that cell wall components of Histoplasma capsulatum vary among fungal isolates, and one of the major differences is the presence or absence of α-(1,3)-glucan, classifying wild-type fungi as chemotypes II or I, respectively. The present work shows that an isolate of H. Read More

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November 2020

Postpartum histoplasmosis in an HIV-negative woman: a case report and phylogenetic characterization by internal transcribed spacer region analysis.

Rev Soc Bras Med Trop 2020 27;53:e20190364. Epub 2020 Jan 27.

Departamento de Saúde Comunitária, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.

The present report describes the first case of postpartum disseminated histoplasmosis in a 24-year-old HIV-negative woman. On the tenth day after vaginal delivery, the patient presented with dyspnea, fever, hypotension, tachycardia, and painful hepatomegaly. Yeast-like Histoplasma capsulatum features were isolated in the buffy coat. Read More

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Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions.

Braz J Infect Dis 2020 Jan - Feb;24(1):44-50. Epub 2020 Jan 25.

Universidad Nacional Autónoma de México (UNAM), Facultad de Medicina, Departamento de Microbiología-Parasitología, Ciudad de México, Mexico. Electronic address:

The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Read More

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Frequency of the Mating-Type (MAT1) in Histoplasma capsulatum Isolates from Buenos Aires, Argentina.

Mycopathologia 2020 Feb 31;185(1):169-174. Epub 2019 Oct 31.

Centro de Micología, Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2150, Piso 11, Buenos Aires, Argentina.

Sex is genetically determined in Histoplasma capsulatum, governed by a sex-specific region in the genome called the mating-type locus (MAT1). We investigate the distribution of isolates of two H. capsulatum mating types in the clades circulating in Buenos Aires, Argentina. Read More

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February 2020

Novel clinical and dual infection by Histoplasma capsulatum genotypes in HIV patients from Northeastern, Brazil.

Sci Rep 2019 08 13;9(1):11789. Epub 2019 Aug 13.

Instituto Nacional de Infectologia Evandro Chagas (INI), FIOCRUZ - Fundação Oswaldo Cruz, Laboratório de Micologia, 21045-900, Rio de Janeiro, RJ, Brazil.

Histoplasmosis is a worldwide-distributed deep mycosis that affects healthy and immunocompromised hosts. Severe and disseminated disease is especially common in HIV-infected patients. At least 11 phylogenetic species are recognized and the majority of diversity is found in Latin America. Read More

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Molecular epidemiology of Colombian isolates obtained from human and chicken manure samples.

Heliyon 2019 Jul 23;5(7):e02084. Epub 2019 Jul 23.

Grupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Antioquia, Colombia.

The thermally dimorphic fungus is the causative agent of histoplasmosis, one of the most prevalent endemic mycosis in the Americas. In tropical regions, agro-ecosystems require organic matter replacement, therefore, the use of organic fertilizers has increased disregarding the fact that certain number of such fertilizers might be contaminated with the fungus, and with their handling resulting in human cases and even outbreaks of histoplasmosis. Additionally, in Colombia, chicken manure is the most common raw material used in the production of organic fertilizers. Read More

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Analyses of the genetic diversity and population structures of Histoplasma capsulatum clinical isolates from Mexico, Guatemala, Colombia and Argentina, using a randomly amplified polymorphic DNA-PCR assay.

Epidemiol Infect 2019 01;147:e204

Departamento de Microbiología-Parasitología,Unidad de Micología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM),Mexico City,Mexico.

We studied the genetic diversity and the population structure of human isolates of Histoplasma capsulatum, the causative agent of histoplasmosis, using a randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) assay to identify associations with the geographic distribution of isolates from Mexico, Guatemala, Colombia and Argentina. The RAPD-PCR pattern analyses revealed the genetic diversity by estimating the percentage of polymorphic loci, effective number of alleles, Shannon's index and heterozygosity. Population structure was identified by the index of association (IA) test. Read More

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January 2019

Immunohistochemical Cross-Reactivity Between Arthrographis kalrae and Highly Pathogenic Coccidioides posadasii, Histoplasma capsulatum, and Paracoccidioides Fungal Species.

Mycopathologia 2019 Jun 14;184(3):393-402. Epub 2019 Jun 14.

Ryukyu Animal Medical Center, Yone 5-27, Tomigusuku, Okinawa, 901-0224, Japan.

Recently, we have reported serological cross-reactivity between paracoccidioidomycosis ceti and paracoccidioidomycosis, histoplasmosis, and coccidioidomycosis. However, data on the interaction of Arthrographis kalrae with the above pathogenic fungal infections are lacking. A. Read More

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Extracellular Vesicle-Mediated RNA Release in .

mSphere 2019 03 27;4(2). Epub 2019 Mar 27.

Departments of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA

Eukaryotic cells, including fungi, release extracellular vesicles (EVs). These lipid bilayered compartments play essential roles in cellular communication and pathogenesis. EV composition is complex and includes proteins, glycans, pigments, and RNA. Read More

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A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in .

mSphere 2019 03 20;4(2). Epub 2019 Mar 20.

Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil

Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of and on solid media. Nanoparticle tracking analysis in combination with transmission electron microscopy revealed that and produced EVs in solid media. Read More

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Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients.

Rev Inst Med Trop Sao Paulo 2019 Feb 14;61:e8. Epub 2019 Feb 14.

Fundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Setor Imunodiagnóstico, Rio de Janeiro, Rio de Janeiro, Brazil.

Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Read More

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February 2019

Recent admixture between species of the fungal pathogen .

Evol Lett 2018 Jun 22;2(3):210-220. Epub 2018 Jun 22.

Biology Department University of North Carolina Chapel Hill North Carolina 27599.

Hybridization between species of pathogens has the potential to speed evolution of virulence by providing the raw material for adaptation through introgression or by assembling new combinations of virulence traits. Fungal diseases are a source high morbidity, and remain difficult to treat. Yet the frequency of hybridization between fungal species has rarely been explored, and the functional role of introgressed alleles remains largely unknown. Read More

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Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types.

Mem Inst Oswaldo Cruz 2018 17;113(10):e180340. Epub 2018 Sep 17.

Fundação Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Setor de Imunodiagnóstico, Rio de Janeiro, RJ, Brasil.

Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. Read More

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October 2018

Immunohistochemical Cross-Reactivity Between Paracoccidioides sp. from Dolphins and Histoplasma capsulatum.

Mycopathologia 2018 Oct 30;183(5):793-803. Epub 2018 Aug 30.

Ryukyu Animal Medical Center, Yone 5-27, Tomigusuku, Okinawa, 901-0224, Japan.

Paracoccidioidomycosis ceti is a cutaneous disease of cetaceans caused by uncultivated Paracoccidioides brasiliensis or Paracoccidioides spp. Serological cross-reactions between paracoccidioidomycosis ceti and paracoccidioidomycosis, paracoccidioidomycosis and histoplasmosis, and paracoccidioidomycosis and coccidioidomycosis have been reported before. The present study aimed to detect immunohistochemical cross-reaction between antibodies to Paracoccidioides sp. Read More

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October 2018

A proposal for antifungal epidemiological cut-off values against Histoplasma capsulatum var. capsulatum based on the susceptibility of isolates from HIV-infected patients with disseminated histoplasmosis in Northeast Brazil.

Int J Antimicrob Agents 2018 Aug 26;52(2):272-277. Epub 2018 Mar 26.

Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; College of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.

Epidemiological cut-off values (ECVs) have been used as a tool to detect the acquisition of resistance mechanisms to antifungal drugs. In this context, the objective of this study was to determine the ECVs for classic antifungals against Histoplasma capsulatum var. capsulatum isolates from human immunodeficiency virus (HIV)-infected patients with a diagnosis of disseminated histoplasmosis. Read More

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