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Neuroactive Type-A γ-Aminobutyric Acid Receptor Allosteric Modulator Steroids from the Hypobranchial Gland of Marine Mollusk, .

J Med Chem 2021 May 5. Epub 2021 May 5.

Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.

In a program to identify pain treatments with low addiction potential, we isolated five steroids, conosteroids A-E (-), from the hypobranchial gland of the mollusk . Compounds - were active in a mouse dorsal root ganglion (DRG) assay that suggested that they might be analgesic. A synthetic analogue was used for a detailed pharmacological study. Read More

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sp. nov., a Novel Hyperthermophilic Starch-Degrading Archaeon.

Biology (Basel) 2021 Apr 29;10(5). Epub 2021 Apr 29.

Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, D-24118 Kiel, Germany.

A novel hyperthermophilic archaeon, termed strain T7324, was isolated from a mixed sulfate-reducing consortium recovered from hot water produced from a deep North Sea oil reservoir. The isolate is a strict anaerobic chemo-organotroph able to utilize yeast extract or starch as a carbon source. The genes for a number of sugar degradation enzymes and glutamate dehydrogenase previously attributed to the sulfate reducing strain of the consortium ( strain 7324) were identified in the nearly completed genome sequence. Read More

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Tandem Character of Liquid Hot Water and Deep Eutectic Solvent to Boost Lignocellulose Deconstruction.

ChemSusChem 2021 May 4. Epub 2021 May 4.

Wuhan University of Science and Technology, School of Chemistry and Chemical Engineering, CHINA.

Pretreatment with efficient fractionation, eco-friendliness, and low-cost brings high-security to future biorefinery systems. Synergistic pretreatment is a compelling blueprint to tackle the compact structure of lignocellulose towards a high-level valorization. We designed a stepwise approach, using hydrothermal and deep eutectic solvent (DES) pretreatments, to hierarchically extract hemicelluloses and lignin from poplar, while delivering a cellulose-rich substrate that can be easily enzymatic hydrolysis to obtain fermentable glucose and residual lignin. Read More

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Analgesic and anti-inflammatory potential of Lupeol isolated from Indian traditional medicinal plant Crateva adansonii screened through in vivo and in silico approaches.

J Genet Eng Biotechnol 2021 May 4;19(1):62. Epub 2021 May 4.

Department of Biotechnology, Yeungnam University, Gyeongsan, South Korea.

Background: Lupeol, a triterpene bioactive compound isolated from Indian traditional plant Crateva adansonii acted as promising and alternative anti-inflammatory agent to treatments of diseases related to inflammation. The inflammatory process in the body serves an important function in the control and repair of injury. However, it is self-perpetuating in number of disease conditions, which must be prevented and treated. Read More

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Geographic Disparities in Late-Stage Breast Cancer Diagnosis Rates and Their Persistence Over Time.

J Womens Health (Larchmt) 2021 Apr 29. Epub 2021 Apr 29.

Division of Cancer Prevention and Control, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Other than skin cancer, breast cancer is the most common cancer in the United States. Lower uptake of mammography screening is associated with higher rates of late-stage breast cancers. This study aims to show geographic patterns in the United States, where rates of late-stage breast cancer are high and persistent over time, and examines factors associated with these patterns. Read More

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Harnessing the Rhizosphere of the Halophyte Grass for Halophilic Plant-Growth-Promoting Fungi and Evaluation of Their Biostimulant Activities.

Plants (Basel) 2021 Apr 16;10(4). Epub 2021 Apr 16.

Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.

Hydroponic systems have gained interest and are increasingly used in hot and dry desert areas. Numbers of benefits are offered by hydroponic systems such as the ability to save water, enhance nutrients use efficiency, easy environmental control, and prevention of soil-borne diseases. However, the high consumption of chemical fertilizers for nutrient solution and the sensitivity of closed hydroponic systems to salinity are issues that need solutions. Read More

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Site-Isolated Azobenzene-Containing Metal-Organic Framework for Cyclopalladated Catalyzed Suzuki-Miyuara Coupling in Flow.

ACS Appl Mater Interfaces 2021 Apr 29. Epub 2021 Apr 29.

Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, United States.

Sites isolation of active metals centers, systematically studied in homogeneous systems, has been an alternative to develop low metal consuming, highly active next generation catalysts in heterogeneous condition. Because of the high porosity and facile synthetic procedures, MOF-based catalysts are excellent candidates for heterogenization of well-defined homogeneous catalysts. Herein, we report the direct Pd coordination on the azobenzene linker within a MOF catalyst through a postsynthetic modification method for a Suzuki-Miyaura coupling reaction. Read More

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Mobile genetic elements mediate the mixotrophic evolution of novel Alicyclobacillus species for acid mine drainage adaptation.

Environ Microbiol 2021 Apr 29. Epub 2021 Apr 29.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410006, China.

Alicyclobacillus species inhabit diverse environments and have adapted to broad ranges of pH and temperature. However, their adaptive evolutions remain elusive, especially regarding the role of mobile genetic elements (MGEs). Here, we characterized the distributions and functions of MGEs in Alicyclobacillus species across five environments, including acid mine drainage (AMD), beverages, hot springs, sediments, and soils. Read More

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[Methanotrophs bacteria in special environment: A review.]

Ying Yong Sheng Tai Xue Bao 2021 Apr;32(4):1509-1517

College of Resource and Environmental Engineering, Guizhou University, Guiyang 550025, China.

Methanotrophs are important and unique prokaryotes widely distributed in the nature. They can oxidize methane and live at a moderate temperature in the conditions withpH=7. In addition, many methanotrophs have been isolated from various special conditions within a wide range of pH value and temperature, including acid sphagnum moss wetlands, acid forest soils, hot springs and high-temperature geothermal areas near volcanic craters, soda-saline lakes, and marine sediments. Read More

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Genomic Insight of FL18 Isolated From an Arsenic-Rich Hot Spring.

Front Microbiol 2021 8;12:639697. Epub 2021 Apr 8.

Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Naples, Italy.

Extreme environments are excellent places to find microorganisms capable of tolerating extreme temperature, pH, salinity pressure, and elevated concentration of heavy metals and other toxic compounds. In the last decades, extremophilic microorganisms have been extensively studied since they can be applied in several fields of biotechnology along with their enzymes. In this context, the characterization of heavy metal resistance determinants in thermophilic microorganisms is the starting point for the development of new biosystems and bioprocesses for environmental monitoring and remediation. Read More

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Complete Genome Sequences of Thermus thermophilus Strains HB5002 and HB5008, Isolated from Mine Hot Spring in Japan.

Microbiol Resour Announc 2021 Apr 22;10(16). Epub 2021 Apr 22.

RIKEN SPring-8 Center, Sayo, Hyogo, Japan

We isolated strains HB5002 and HB5008 from Mine Hot Spring in Japan. Whole-genome sequencing revealed that they showed ∼100% average nucleotide identity to each other, ≥98.53% to the strains originating from the same spot but ≤97. Read More

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[Epidemiological characteristics and outcome analysis of 266 patients with inhalation injuries combined with total burn area less than 30% total body surface area].

Zhonghua Shao Shang Za Zhi 2021 Apr;37(4):340-349

Burn Institute of PLA, Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Research Unit of Key Techniques for Treatment of Burns and Combined Burns and Trauma Injury, Chinese Academy of Medical Sciences, Shanghai 200433, China.

To explore the epidemiological characteristics and treatment outcomes of patients with inhalation injuries combined with total burn area less than 30% total body surface area (TBSA). A retrospective observational study was performed on medical records of 266 patients with inhalation injuries combined with total burn area less than 30%TBSA who were admitted to the First Affiliated Hospital of Naval Medical University from January 2008 to December 2016 and met the inclusion criteria. The following statistical data of the patients were collected, including gender, age, injury site, injurious factors of inhalation injury, degree of inhalation injury, combined total burn area, tracheotomy, time of tracheotomy, mechanical ventilation, whether stayed in intensive care unit (ICU) or not, microbial culture results of bronchoalveolar lavage fluid, length of hospital stay, length of ICU stay, mechanical ventilation days, and respiratory tract infections. Read More

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The exploitation of thermophile resources in hot springs: fluorescent carbon dots derived from for multicolour cellular imaging and selectivity detection of heavy metals.

Anal Methods 2021 04;13(15):1810-1815

Faculty of Land Resource Engineering, Kunming University of Science and Technology, Kunming 650500, China.

Microbial biomass, as an environmentally friendly resource, has attracted considerable attention as a green biomaterial for the production of unique and functionalised CDs; however, further exploration is required to characterise CDs derived from bacteria. In this study, a green biomaterial (fluorescence CDs-HS18) was successfully synthesised via a hydrothermal method from Ureibacillus thermosphaericus HS-18 specimens isolated from a hot spring. The prepared CDs-HS18 possess excellent photo-physical properties, outstanding fluorescence capabilities, and high biocompatibility, which make them desirable candidates for multi-mode imaging applications. Read More

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[Epidemiological characteristics and outcome analysis of inhalation injury patients combined with burn area less than 30% total body surface area in a single center from 2008 to 2016].

Zhonghua Shao Shang Za Zhi 2021 Mar 24;37:1-10. Epub 2021 Mar 24.

Burn Institute of PLA, Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Research Unit of Key Techniques for Treatment of Burns and Combined Burns and Trauma Injury, Chinese Academy of Medical Sciences, Shanghai 200433, China.

To explore the epidemiological characteristics and treatment outcomes of inhalation injury patients combined with burn area less than 30% total body surface area (TBSA) admitted to the First Affiliated Hospital of Naval Medical University. Retrospective observational study with performed on medical records of 266 inhalation injury patients combined with burn area less than 30% TBSA who were admitted to the First Affiliated Hospital of Naval Medical University from January 2008 to September 2016 and met the inclusion criteria. The gender, age, injured site, injurious factors of inhalation injury, degree of inhalation injury, combined total burn area, way of tracheotomy, time of tracheotomy, whether conducted mechanical ventilation or not, whether in intensive care unit (ICU) or not, microbial culture results of bronchoalveolar lavage, total hospitalization days, ICU days, mechanical ventilation days, and whether respiratory infections occurred or not. Read More

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In Vivo Anti-inflammatory, Analgesic, and Sedative Studies of the Extract and Naphthoquinone Isolated from (Persimmon).

ACS Omega 2021 Apr 1;6(14):9852-9856. Epub 2021 Apr 1.

Department of Chemistry University of Swabi, Swabi, Anbar 23430, KPK, Pakistan.

Anti-inflammatory, analgesic, and sedative medicine is used with numerous side effects, including peptic ulcer, headache, addiction, and other complications. In this regard, discovery research is undergoing a process to discover effective, safe, and economical drugs with no side effects. The aim of this study was to assess chloroform extracts and isolated compounds for anti-inflammatory, analgesic, and sedative activities in animal models. Read More

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16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients.

PeerJ 2021 2;9:e10995. Epub 2021 Apr 2.

Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

Microorganisms in geothermal springs can offer insights into the fundamental and applied study of extremophiles. However, low microbial abundance and culturing requirements limit the ability to analyze microbial diversity in these ecosystems. In this study, culture-dependent and culture-independent techniques were used to analyze sediment samples from the non-volcanic Tatta Pani hot springs in district Poonch of Azad Kashmir. Read More

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Sub-second Time-resolved Surface Enhanced Raman Spectroscopy Reveals Dynamic CO Intermediates during Electrochemical CO2 Reduction on Copper.

Angew Chem Int Ed Engl 2021 Apr 14. Epub 2021 Apr 14.

Utrecht University, Dept. of Inorganic Chemistry and Catalysis, Universiteitsweg 99, 3584 CG, Utrecht, NETHERLANDS.

Electrocatalytic reduction of carbon dioxide (CO2) into value-added products (e.g., ethylene) is a promising approach for greenhouse gas mitigation, but many details of electrocatalytic CO2 reduction reactions (CO2RR) remain elusive. Read More

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Does pericentral mu-rhythm "power" corticomotor excitability? - A matter of EEG perspective.

Brain Stimul 2021 Apr 20;14(3):713-722. Epub 2021 Apr 20.

Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital, Amager and Hvidovre, Hvidovre, Denmark; Department of Neurology, Copenhagen University Hospital Bispebjerg, Copenhagen, Denmark; Institute for Clinical Medicine, Faculty of Medical and Health Sciences, University of Copenhagen, Copenhagen, Denmark.

Background: Electroencephalography (EEG) and single-pulse transcranial magnetic stimulation (spTMS) of the primary motor hand area (M1-HAND) have been combined to explore whether the instantaneous expression of pericentral mu-rhythm drives fluctuations in corticomotor excitability, but this line of research has yielded diverging results.

Objectives: To re-assess the relationship between the mu-rhythm power expressed in left pericentral cortex and the amplitude of motor potentials (MEP) evoked with spTMS in left M1-HAND.

Methods: 15 non-preselected healthy young participants received spTMS to the motor hot spot of left M1-HAND. Read More

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Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors.

Front Endocrinol (Lausanne) 2021 25;12:622647. Epub 2021 Mar 25.

Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, Aurora, CO, United States.

Proinsulin is an abundant protein that is selectively expressed by pancreatic beta cells and has been a focus for development of antigen-specific immunotherapies for type 1 diabetes (T1D). In this study, we sought to comprehensively evaluate reactivity to preproinsulin by CD4 T cells originally isolated from pancreatic islets of organ donors having T1D. We analyzed 187 T cell receptor (TCR) clonotypes expressed by CD4 T cells obtained from six T1D donors and determined their response to 99 truncated preproinsulin peptide pools, in the presence of autologous B cells. Read More

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Identification and quantification of oleanane triterpenoid saponins and potential analgesic and anti-inflammatory activities from the roots and rhizomes of .

J Ginseng Res 2021 Mar 18;45(2):305-315. Epub 2020 May 18.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

Background: represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from . Read More

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Planning and Design of a Full-Outer-Air-Intake Natural Air-Conditioning System for Medical Negative Pressure Isolation Wards.

J Healthc Eng 2021 25;2021:8872167. Epub 2021 Mar 25.

Department of Electronic Engineering, National Taipei University of Technology, No. 1 Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan.

In the beginning of 2020, the coronavirus (COVID-19) pandemic started to spread globally, causing panic to the lives of people around the world; many countries executed lockdown of cities or even total lockdown of the entire countries. The coronavirus disease (COVID-19) is transmitted via air droplets. In medical environments that use traditional hermetic ventilation systems, medical personnel who come in contact with patients are more susceptible to infection compared to regular staff; therefore, the air flow and air quality of hermetic negative pressure isolation wards are highly critical. Read More

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Effects of scorpion venom heat-resistant peptide on the hippocampal neurons of kainic acid-induced epileptic rats.

Braz J Med Biol Res 2021 2;54(5):e10717. Epub 2021 Apr 2.

Department of Neurosurgery, The 900th Hospital of Joint Logistic Support Force, PLA, Fuzhou, China.

Scorpion venom is a Chinese medicine for epilepsy treatment, but the underlying mechanism is not clear. Scorpion venom heat-resistant peptide (SVHRP), a peptide isolated from the venom of Buthus martensii Karsch, has an anti-epileptic effect by reducing seizure behavior according to a modified Racine scale. The present study aimed to investigate the molecular mechanism of SVHRP on temporal lobe epilepsy. Read More

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Are pathogenic Legionella non-pneumophila a common bacteria in Water Distribution Networks?

Water Res 2021 May 7;196:117013. Epub 2021 Mar 7.

Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid, España. Electronic address:

The present study analyzes at the national level, the presence of circulating Legionella in the artificial aquatic systems of different facilities of all of them state-owned centers throughout Spain for 12 months. 1754 water samples from various state-owned centers were collected from January to December 2014. Samples were collected from the cooling towers and evaporative condensers (CTC), and water distribution networks such as domestic hot water (DHW), cold water for human consumption (CW), sprinkler irrigation systems (SIS), fire sprinkler systems (FSS), and water from decorative fountains (DF). Read More

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Macromolecular Model of the Pectic Polysaccharides Isolated from the Bark of Norway Spruce ().

Polymers (Basel) 2021 Mar 31;13(7). Epub 2021 Mar 31.

Division of Wood Chemistry and Pulp Technology, Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Teknikringen 56, SE-100 44 Stockholm, Sweden.

The bark of Norway spruce () contains up to 13% pectins that can be extracted by pressurized hot water, which constitute a valuable renewable resource in second-generation lignocellulosic biorefineries. This article proposes, for the first time, structural molecular models for the pectins present in spruce bark. Pectin fractions of tailored molar masses were obtained by fractionation of the pressurized hot water extract of the inner bark using preparative size-exclusion chromatography. Read More

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Peppers: A "Hot" Natural Source for Antitumor Compounds.

Molecules 2021 Mar 10;26(6). Epub 2021 Mar 10.

Laboratory of Design and Synthesis of Bioactive Substances, Department of Pharmacy, University of São Paulo, Prof. Lineu Prestes Avenue 580, Bl.13, Butantã, SP 05508-900, Brazil.

, , and are the main genera of peppers consumed worldwide. The traditional use of peppers by either ancient civilizations or modern societies has raised interest in their biological applications, including cytotoxic and antiproliferative effects. Cellular responses upon treatment with isolated pepper-derived compounds involve mechanisms of cell death, especially through proapoptotic stimuli in tumorigenic cells. Read More

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Isolation, Characterization, and Medicinal Potential of Polysaccharides of .

Molecules 2021 Mar 8;26(5). Epub 2021 Mar 8.

Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Mushroom polysaccharides are active medicinal compounds that possess immune-modulatory and anticancer properties. Currently, the mushroom polysaccharides krestin, lentinan, and polysaccharopeptides are used as anticancer drugs. They are an unexplored source of natural products with huge potential in both the medicinal and nutraceutical industries. Read More

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Purification, structural characterization and immunostimulatory activity of polysaccharides from Umbilicaria esculenta.

Int J Biol Macromol 2021 Mar 30;181:743-751. Epub 2021 Mar 30.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China. Electronic address:

In this study, an active component UP1-1 was isolated from Chinese Huangshan Umbilicaria esculenta via hot water extraction and purified by anion-exchange and gel-filtration chromatography. UP1-1 mainly composed of galactose, mannose and glucose in a molar ratio of 0.8:1. Read More

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Advances in Detecting Low Prevalence Somatic Promoter Mutations in Papillary Thyroid Carcinoma.

Front Endocrinol (Lausanne) 2021 12;12:643151. Epub 2021 Mar 12.

Genetic Bases of Thyroid Tumors Laboratory, Department of Morphology and Genetics, Division of Genetics, Universidade Federal de São Paulo, São Paulo, Brazil.

Background: Two recurrent (telomerase reverse transcriptase) promoter mutations, C228T and C250T, have been reported in thyroid carcinomas and were correlated with high-risk clinicopathological features and a worse prognosis. Although far more frequent in the poorly differentiated and undifferentiated thyroid cancer, the promoter mutations play a significant role on PTC recurrence and disease-specific mortality. However, the prevalence varies considerably through studies and it is uncertain if these differences are due to population variation or the methodology used to detect mutations. Read More

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Gene expression of microbial gelatinase activity for porcine gelatine identification.

Food Chem 2021 Sep 13;355:129586. Epub 2021 Mar 13.

Malaysia Genome Institute (MGI), Jalan Bangi, 43000 Kajang, Selangor, Malaysia.

In order to invent a porcine gelatine detection device using microbial resources, bacterial enzymes with a preference towards porcine gelatine and their candidate genes were evaluated. Five (n = 5) bacterial strains isolated from hot spring water and wet clay, Malaysia were screened for their gelatinase activity. The gelatinase enzyme was extracted and purified using ammonium sulphate precipitation prior to performing gelatinase assay on porcine, bovine and fish gelatine medium substrates. Read More

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September 2021

Multidrug-, methicillin-, and vancomycin-resistant Staphylococcus aureus isolated from ready-to-eat meat sandwiches: An ongoing food and public health concern.

Int J Food Microbiol 2021 May 17;346:109165. Epub 2021 Mar 17.

Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

Methicillin-resistant S. aureus (MRSA) and their antimicrobial resistance pose exacerbating global health threats and endangering everyone. Thus, the prevalence, molecular characterization of virulence genes, and antimicrobial resistance patterns of strains isolated from 225 beef burger and hot dog sandwiches vended in Mansoura city, Egypt were determined. Read More

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