164,577 results match your criteria isolated dna


Novel hyperthermophilic crenarchaeon Infirmifilum lucidum gen. nov. sp. nov., reclassification of Thermofilum uzonense as Infirmifilum uzonense comb. nov. and assignment of the family Thermofilaceae to the order Thermofilales ord. nov.

Syst Appl Microbiol 2021 Jul 9;44(4):126230. Epub 2021 Jul 9.

Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS, 7/2 Prospekt 60-letiya Oktyabrya, 117312 Moscow, Russia.

A novel hyperthermophilic crenarchaeon, strain 3507LT, was isolated from a terrestrial hot spring near Tinguiririca volcano, Chile. Cells were non-motile thin, slightly curved filamentous rods. It grew at 73-93 °C and pH range of 5 to 7. Read More

View Article and Full-Text PDF

Monomeric C-reactive protein promotes platelets to release mitochondrial DNA in anti-neutrophil cytoplasmic antibody-associated vasculitis.

Mol Immunol 2021 Jul 19;137:228-237. Epub 2021 Jul 19.

Department of Nephrology, Tianjin Medical University General Hospital, Tianjin, 300052, China. Electronic address:

Although high level of circulating C-reactive protein (pCRP) is considered as a biomarker for disease activity, the significance of CRP in the pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) has not been clarified. We once reported in AAV, pentameric CRP (pCRP) could dissociate into monomeric CRP (mCRP) and activate platelets. Recent studies have demonstrated that the activated platelets can release mitochondrial DNA (mtDNA). Read More

View Article and Full-Text PDF

Fusaricide is a Novel Iron Chelator that Induces Apoptosis through Activating Caspase-3.

J Nat Prod 2021 Jul 22. Epub 2021 Jul 22.

State Key Laboratory of Applied Organic Chemistry (SKLAOC), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu 730000, P. R. China.

Nonsmall cell lung cancer (NSCLC) has been a fatal and refractory disease worldwide. Novel therapeutic developments based on fundamental investigations of anticancer mechanisms underlie substantial foundations to win the fight against cancer diseases. In this study, we isolated a natural product fusaricide (FCD) from an endophytic fungus of , identified as . Read More

View Article and Full-Text PDF

sp. nov., isolated from poultry feather waste.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, Free State, South Africa.

A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic, yellow-pigmented bacterium was isolated from chicken feather waste collected from an abattoir in Bloemfontein, South Africa. A polyphasic taxonomy study was used to describe and name the bacterial isolate, strain 1_F178. The 16S rRNA gene sequence analysis and sequence comparison data indicated that strain 1_F178 was a member of the genus and was closely related to (99. Read More

View Article and Full-Text PDF

sp. nov., isolated from marine sediment.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain (CAU 1508) was isolated from marine sediment collected in the Republic of Korea. Growth was observed at 10-45 °C (optimum, 30 °C), pH 4.0-11. Read More

View Article and Full-Text PDF

sp. nov., isolated from Indian Ocean marine sediment.

Int J Syst Evol Microbiol 2021 Jul;71(7)

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.

A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148, was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 °C, pH 8.0 and in the presence of 1-3 % (w/v) NaCl. Read More

View Article and Full-Text PDF

sp. nov. and sp. nov., isolated from desert biocrust.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.

Two novel Gram-staining-negative, aerobic, cocci-shaped, non-motile, non-spore forming, pink-pigmented bacteria designated strains T6 and T18, were isolated from a biocrust (biological soil crust) sample from the vicinity of the Tabernas Desert (Spain). Both strains were catalase-positive and oxidase-negative, and grew under mesophilic, neutrophilic and non-halophilic conditions. According to the 16S rRNA gene sequences, strains T6 and T18 showed similarities with CGMCC 1. Read More

View Article and Full-Text PDF

Genome-Wide DNA (hydroxy)methylation reveals the individual epigenetic landscape importance on osteogenic phenotype acquisition in periodontal ligament cells.

J Periodontol 2021 Jul 22. Epub 2021 Jul 22.

Department of Dentistry, University of Taubaté, Taubaté, Brazil.

Background: Mesenchymal cells' biology has been an important investigative tool to maximize bone regeneration through tissue engineering. Here we used mesenchymal cells from periodontal ligament (PDLCs) with high (h-) and low (l-) osteogenic potential, isolated from different donors, to investigate the impact of the individual epigenetic and transcriptional profiles on the osteogenic potential.

Materials And Methods: Genome-wide and gene-specific DNA (hydroxy)methylation, mRNA expression and immunofluorescence analysis were carried out in h- and l-PDLCs at DMEM (non-induced to osteogenesis) and OM (induced - 3 and 10 days of osteogenic differentiation) groups in vitro. Read More

View Article and Full-Text PDF

Early detection of metastatic uveal melanoma by the analysis of tumor-specific mutations in cell-free plasma DNA.

Cancer Med 2021 Jul 21. Epub 2021 Jul 21.

Institute of Human Genetics, University Hospital Essen, University Duisburg-Essen, Essen, Germany.

Background: Eye salvaging therapy of malignant melanomas of the uvea can preserve the eye in most cases, but still about half of patients die from metastatic disease. Previous analyses of cell-free DNA from plasma had shown detectable levels of tumor-specific GNAQ/GNA11 mutations in patients with the clinical diagnosis of progressive disease. However, data on the time span that elapses from the detection of ctDNA in plasma to the clinical detection of metastases (diagnostic lead time) are missing. Read More

View Article and Full-Text PDF

Aegicerativicinus sediminis gen. nov., sp. nov., a novel carotenoid-producing marine bacterium in the family Flavobacteriaceae.

Antonie Van Leeuwenhoek 2021 Jul 22. Epub 2021 Jul 22.

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.

A novel bright-yellow pigmented bacterial strain SM2-F was isolated from a mangrove sediment collected at the mangrove coast of Luoyang estuary, Quanzhou, China. Strain SM2-F was Gram-stain-negative, catalase-weak positive, oxidase-positive, rod-shaped, non-flagellated and non-motile. Growth of strain SM2-F was observed at 20-40 °C (optimum, 30 °C), pH 6. Read More

View Article and Full-Text PDF

Identification and monitoring of Copy Number Variants (CNV) in monoclonal gammopathy.

Cancer Biol Ther 2021 Jul 21:1-9. Epub 2021 Jul 21.

Interdisciplinary Department of Medicine, University of Bari Aldo Moro, Bari, Italy.

Monoclonal gammopathy of undetermined significance (MGUS) represents the pre-clinical stage of Multiple Myeloma (MM) with the 5% of MGUS progresses to MM. Although the progression from MGUS to MM has not been completely characterized, it is possible to monitor the DNA modifications of patients diagnosed with MGUS to detect early specific genomic abnormalities, including copy number variations (CNV). The CNVs of chromosome 1q and chromosome 13q are associated with a worse prognosis in MM. Read More

View Article and Full-Text PDF

Burkholderia perseverans sp. nov., a bacterium isolated from the Restinga ecosystem, is a producer of volatile and diffusible compounds that inhibit plant pathogens.

Braz J Microbiol 2021 Jul 21. Epub 2021 Jul 21.

Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.

Gram-negative, aerobic, rod-shaped, non-spore-forming, motile bacteria, designated CBAS 719, CBAS 732 and CBAS 720 were isolated from leaf litter samples, collected in Espírito Santo State, Brazil, in 2008. Sequences of the 16S rRNA, gyrB, lepA and recA genes showed that these strains grouped with Burkholderia plantarii LMG 9035, Burkholderia gladioli LMG 2216 and Burkholderia glumae LMG 2196 in a clade of phytopathogenic Burkholderia species. Digital DNA-DNA hybridization experiments and ANI analyses demonstrated that strain CBAS 719 represents a novel species in this lineage that is very closely related with B. Read More

View Article and Full-Text PDF

Activation of Cryptic Secondary Metabolite Biosynthesis in Bamboo Suspension Cells by a Histone Deacetylase Inhibitor.

Appl Biochem Biotechnol 2021 Jul 21. Epub 2021 Jul 21.

Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.

Plants have evolved a diverse array of secondary metabolite biosynthetic pathways. Undifferentiated plant cells, however, tend to biosynthesize secondary metabolites to a lesser extent and sometimes not at all. This phenomenon in cultured cells is associated with the transcriptional suppression of biosynthetic genes due to epigenetic alterations, such as low histone acetylation levels and/or high DNA methylation levels. Read More

View Article and Full-Text PDF

In vitro cellular and proteome assays identify Wnt pathway and CDKN2A-regulated senescence affected in mesenchymal stem cells from mice after a chronic LD gamma irradiation in utero.

Radiat Environ Biophys 2021 Aug 21;60(3):397-410. Epub 2021 Jul 21.

Institute of Radiation Biology, Helmholtz Zentrum München GmbH, German Research Center for Environmental Health GmbH (HMGU), Ingolstaedter Landstraße 1, 85764, Neuherberg, Germany.

Reliable data on the effects of chronic prenatal exposure to low dose (LD) of ionizing radiation in humans are missing. There are concerns about adverse long-term effects that may persist throughout postnatal life of the offspring. Due to their slow cell cycle kinetics and life-long residence time in the organism, mesenchymal stem cells (MSCs) are more susceptible to low level genotoxic stress caused by extrinsic multiple LD events. Read More

View Article and Full-Text PDF

Newborn Screening for Severe Combined Immunodeficiency: Do Preterm Infants Require Special Consideration?

Int J Neonatal Screen 2021 Jul 8;7(3). Epub 2021 Jul 8.

Wisconsin State Laboratory of Hygiene, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.

The Wisconsin Newborn Screening (NBS) Program began screening for severe combined immunodeficiency (SCID) in 2008, using real-time PCR to quantitate T-cell receptor excision circles (TRECs) in DNA isolated from dried blood NBS specimens. Prompted by the observation that there were disproportionately more screening-positive cases in premature infants, we performed a study to assess whether there is a difference in TRECs between full-term and preterm newborns. Based on de-identified SCID data from 1 January to 30 June 2008, we evaluated the TRECs from 2510 preterm newborns (gestational age, 23-36 weeks) whose specimens were collected ≤72 h after birth. Read More

View Article and Full-Text PDF

Description of sp. nov., harboring antibiotic- and toxic compound-resistace genes, isolated from poultry manure.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.

A polyphasic taxonomic approach was used to characterize a Gram-stain-negative bacterium, designated strain CC-YST696, harbouring antibiotic- and toxic compound-resistace genes, isolated from poultry manure in Taiwan. Cells of CC-YST696 were short rods, motile with polar flagella, catalase- and oxidase-positive. Optimal growth occurred at 30 °С, pH 9 and with 1 % NaCl. Read More

View Article and Full-Text PDF

Description of sp. nov., isolated from deep sea sediment from the Mariana Trench, and emended description of the genus .

Int J Syst Evol Microbiol 2021 Jul;71(7)

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Institute of Oceanology, Qingdao, PR China.

In this study, we reported a Gram-stain-negative, orange-coloured, rod-shaped, motile and faculatively anaerobic bacterium named strain PB63, which was isolated from the deep-sea sediment from the Mariana Trench. Growth of PB63 occurred at 10-35 °C (optimum, 28 °C), pH 5.0-8. Read More

View Article and Full-Text PDF

Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting .

J Virol 2021 Jul 21:JVI0239120. Epub 2021 Jul 21.

Department of Animal Science, Texas A&M University, Kleberg Center, Suite 133 2471, 474 Olsen Blvd, College Station, TX 77843, USA.

The majority of previously described bacteriophages belong to three major groups: P68-like podophages, Twort-like or K-like myophages, and a more diverse group of temperate siphophages. Here we present three novel "jumbo" phages: MarsHill, Madawaska, and Machias. These phages were isolated from swine production environments in the United States and represent a novel clade of myophage. Read More

View Article and Full-Text PDF

RNA Sequencing of CD4 T Cells in Relapsing-Remitting Multiple Sclerosis Patients at Relapse: Deciphering the Involvement of Novel genes and Pathways.

J Mol Neurosci 2021 Jul 21. Epub 2021 Jul 21.

Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poorsina St, 16 Azar St., Enghelab Ave, Tehran, Iran.

CD4 T cells are known as a noteworthy potential modulator of inflammation in multiple sclerosis (MS). In the current study, we investigated the transcriptome profile of CD4 T cells in patients with relapsing-remitting MS (RRMS) at the relapse phase. We performed RNA sequencing of CD4 T cells isolated from four relapsing-remitting MS (RRMS) patients at the relapse phase and four age- and sex-matched healthy controls. Read More

View Article and Full-Text PDF

Gene Regulatory Network of Human GM-CSF-Secreting T Helper Cells.

J Immunol Res 2021 3;2021:8880585. Epub 2021 Jul 3.

Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, ki.se Karolinska University Hospital & Science for Life Laboratory, 17176 Solna, Stockholm, Sweden.

GM-CSF produced by autoreactive CD4-positive T helper cells is involved in the pathogenesis of autoimmune diseases, such as multiple sclerosis. However, the molecular regulators that establish and maintain the features of GM-CSF-positive CD4 T cells are unknown. In order to identify these regulators, we isolated human GM-CSF-producing CD4 T cells from human peripheral blood by using a cytokine capture assay. Read More

View Article and Full-Text PDF

Clinical and Molecular Characteristics of Carbapenem-Resistant Hypervirulent Isolates in a Tertiary Hospital in Shanghai, China.

Infect Drug Resist 2021 14;14:2697-2706. Epub 2021 Jul 14.

Department of Clinical Laboratory Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai, People's Republic of China.

Background: The convergence of carbapenem-resistance and hypervirulence in has led to a significant public health challenge. In recent years, there have been more and more reports on carbapenem-resistant hypervirulent (CR-hvKP) isolates.

Materials And Methods: Clinical data of patients infected with CR-hvKP from January 2019 to December 2020 in a tertiary hospital were retrospectively evaluated. Read More

View Article and Full-Text PDF

Genomic Insights into a Complete Deletion of the mgrB Locus in Colistin-Resistant Klebsiella pneumoniae ST2268 Isolated from a Human Infection.

J Glob Antimicrob Resist 2021 Jul 17. Epub 2021 Jul 17.

Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China. Electronic address:

Objectives: The emergence of colistin-resistant Klebsiella pneumoniae (CoRKp) is a serious public health issue because colistin is the last line of defense against infections caused by multidrug-resistant Gram-negative bacteria. Here, we generated a draft genome sequence for CoRKp strain P094-1, isolated from a sputum sample from an infected patient.

Methods: Whole genomic DNA of strain P094-1 was sequenced using the Pacific Biosciences platform. Read More

View Article and Full-Text PDF

Museum genomics reveals the Xerces blue butterfly () was a distinct species driven to extinction.

Biol Lett 2021 Jul 21;17(7):20210123. Epub 2021 Jul 21.

Department of Entomology, Cornell University, Ithaca, NY 14853, USA.

The last Xerces blue butterfly was seen in the early 1940s, and its extinction is credited to human urban development. This butterfly has become a North American icon for insect conservation, but some have questioned whether it was truly a distinct species, or simply an isolated population of another living species. To address this question, we leveraged next-generation sequencing using a 93-year-old museum specimen. Read More

View Article and Full-Text PDF

sp. nov. and sp. nov., isolated from salt lake and saline soil.

Int J Syst Evol Microbiol 2021 Jul;71(7)

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.

Two halophilic archaeal strains, Gai3-2 and NJ-3-1, were isolated from salt lake and saline soil samples, respectively, collected in PR China. The 16S rRNA gene sequences of the two strains were 97.5% similar to each other. Read More

View Article and Full-Text PDF

gen. nov., sp. nov., a novel bacterium in the family , isolated from desert soil, and reclassification of to a new genus as comb. nov., and transfer of the genera and from the family to the family .

Int J Syst Evol Microbiol 2021 Jul;71(7)

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.

A novel pale orange-coloured bacterium, designated strain SYSU D00532, was isolated from sandy soil collected from the Gurbantunggut desert in Xinjiang, PR China. Cells of strain SYSU D00532 were found to be aerobic, Gram-stain-negative, oxidase-positive, catalase-positive, motile and rod-shaped with a single polar or subpolar flagellum. Growth occurred at 15-45 °C (optimum, 28-37 °C, pH 5. Read More

View Article and Full-Text PDF

sp. nov., a thermophilic bacterium isolated from compost.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.

A Gram-stain-positive, yellow-pigmented, non-motile actinobacterial strain, designated as BIT-GX5, was isolated from a sesame husks compost collected in Beijing, PR China. This bacterium was found to be able to grow in the temperature range from 16 to 50 °C and had an optimal growth temperature at 45 °C. Its taxonomic position was analysed using a polyphasic approach. Read More

View Article and Full-Text PDF

First Report of Maize Ear Rot Caused by Exserohilum rostratum in Hainan Province in Southern China.

Plant Dis 2021 Jul 20. Epub 2021 Jul 20.

Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Dongguan street NO.437, Baoding, Hebei, China, +8613930861039.

Maize (Zea mays L.) is one of three major grain crops in China, with production reaching 261 million tons in 2019(NBS, 2020). Some fungi cause maize ear rot which lead to significant yield and quality losses. Read More

View Article and Full-Text PDF

Antimicrobial activity of bacteria associated with the rhizosphere and phyllosphere of Avena fatua and Brachiaria reptans.

Environ Sci Pollut Res Int 2021 Jul 19. Epub 2021 Jul 19.

School of Biological Sciences, University of the Punjab, Lahore, 54590, Pakistan.

Environmental pollution especially heavy metal-contaminated soils adversely affects the microbial communities associated with the rhizosphere and phyllosphere of plants growing in these areas. In the current study, we identified and characterized the rhizospheric and phyllospheric bacterial strains from Avena fatua and Brachiaria reptans with the potential for antimicrobial activity and heavy metal resistance. A total of 18 bacterial strains from the rhizosphere and phyllosphere of A. Read More

View Article and Full-Text PDF

Litoribacter populi sp. nov., isolated from the soil of a Populus euphratica forest.

Arch Microbiol 2021 Jul 19. Epub 2021 Jul 19.

Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Xinjiang Laboratory of Special Environmental Microbiology, Urumqi, 830091, Xinjiang, China.

A Gram-stain-negative, rod-shaped, and orange-pigmented bacterium, designated XAAS-A1, was isolated from the soil of a Populus euphratica forest. The main respiratory quinone of XAAS-A1 is MK-7, and the principal cellular fatty acids are iso-C, C ω6c, Iso-C 3OH, and summed feature 3 (Cω6c and/or Cω7c). The major polar lipids are phosphatidylethanolamine, phosphatidylcholine, and two unidentified lipids. Read More

View Article and Full-Text PDF

sp. nov. and sp. nov., isolated from Tibetan Plateau wildlife.

Int J Syst Evol Microbiol 2021 Jul;71(7)

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

Four mesophilic and Gram-stain-positive strains (zg-686/zg-691 and HY186/HY189) isolated from Tibetan Plateau wildlife (PR China) belong to the genus according to 16S rRNA gene and genomic sequence-based phylogenetic/genomic results. They have a DNA G+C content range of 67.4-68. Read More

View Article and Full-Text PDF