17 results match your criteria ipa attached

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meCLICK-Seq, a Substrate-Hijacking and RNA Degradation Strategy for the Study of RNA Methylation.

ACS Cent Sci 2020 Dec 29;6(12):2196-2208. Epub 2020 Oct 29.

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

The fates of RNA species in a cell are controlled by ribonucleases, which degrade them by exploiting the universal structural 2'-OH group. This phenomenon plays a key role in numerous transformative technologies, for example, RNA interference and CRISPR/Cas13-based RNA editing systems. These approaches, however, are genetic or oligomer-based and so have inherent limitations. Read More

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December 2020

Interfacial modification by multifunctional octocrylene for high efficiency and stable planar perovskite solar cells.

Chem Commun (Camb) 2020 Jun;56(49):6731-6734

School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China. and Beijing Key Laboratory of Special Melting and Preparation of High-End Metal Materials, University of Science and Technology Beijing, Beijing 100083, China.

Interfacial modification of the perovskite surface with octocrylene (2-ethylhexyl-2-cyano-3,3-diphenyl-2-propenoate, OCT) is capable of enhancing humidity stability and passivating the defects of perovskite films. In this study, octocrylene can be attached to the surface of the perovskite, and the carbonyl group (C[double bond, length as m-dash]O) in octocrylene achieved excellent passivation through the Lewis base electron passivation of Pb2+ ions. By increasing the concentration of the octocrylene/IPA solution, the modified device exhibited an optimal PCE of 20. Read More

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Revisit of solubility of oxytetracycline polymorphs. An old story in the light of new results.

Eur J Pharm Sci 2020 Apr 5;149:105328. Epub 2020 Apr 5.

Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre u. 9., Budapest H-1092 Hungary. Electronic address:

In the literature the therapeutic nonequivalence of oxytetracycline hydrochloride (OTCH) capsules and tablets was attributed to the different aqueous solubility of polymorphs without their comprehensive study. Our aim was to reveal the effects of polymorphism on equilibrium solubility, dissolution kinetics and the supersaturation of two OTCH polymorphs (stable Form A and metastable Form B).The equilibrium solubility was measured in biorelevant pH range 4-7. Read More

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High-throughput metabolomics investigates anti-osteoporosis activity of oleanolic acid via regulating metabolic networks using ultra-performance liquid chromatography coupled with mass spectrometry.

Phytomedicine 2018 Dec 6;51:68-76. Epub 2018 Oct 6.

Department of orthopaedics, The Fourth Hospital attached to Nanchang University, No. 133, Guangchang South Road, Xihu District, Nanchang, Jiangxi 330003, China. Electronic address:

Background: Osteoporosis has brought about heavy socio-economic burden in the morbidity and medical expenses associated with osteoporosis treatment and various restrictions on behavior of their social roles. Oleanolic acid (OA) is an anti-osteoporosis natural product, but molecular mechanisms of therapeutic effect are not still well known.

Purpose: In this study, we explore anti-osteoporosis activity of oleanolic acid and predict the underlying mechanisms by metabolomics strategy. Read More

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December 2018

An in vitro comparison of standard cleaning to a continuous passive disinfection cap for the decontamination of needle-free connectors.

Antimicrob Resist Infect Control 2018 5;7:50. Epub 2018 Apr 5.

University Hospitals Birmingham NHS Foundation Trust, Birmingham, B15 2TH UK.

Background: The optimal decontamination method for needle-free connectors is still unresolved. The objective of this study was to determine if a continuous passive disinfection cap is as effective as standard cleaning for the microbial decontamination of injection ports of two types of needle-free connectors.

Methods: The injection ports of needle-free connectors were inoculated with and allowed to dry. Read More

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Bioinformatics Analysis on Molecular Mechanism of Green Tea Compound Epigallocatechin-3-Gallate Against Ovarian Cancer.

Clin Transl Sci 2017 Jul 23;10(4):302-307. Epub 2017 May 23.

Department of Biological Sciences, National University of Singapore, Singapore, 1175432.

Epigallocatechin-3-gallate (EGCG) is the most abundant and biologically active catechin in green tea, and it exerts multiple effects in humans through mechanisms that remain to be clarified. The present study used bioinformatics to identify possible mechanisms by which EGCG reduces the risk of ovarian cancer. Possible human protein targets of EGCG were identified in the PubChem database, possible human gene targets were identified in the National Center for Biotechnology Information database, and then both sets of targets were analyzed using Ingenuity Pathway Analysis (IPA). Read More

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The psychological health and well-being of emergency medicine consultants in the UK.

Emerg Med J 2017 Jul 13;34(7):430-435. Epub 2016 Oct 13.

Emergency Department, Royal Devon & Exeter Foundation Trust, Exeter, UK.

Objective: To explore the experience of psychological distress and well-being in emergency medicine (EM) consultants.

Methods: A qualitative, interpretative phenomenological analysis (IPA) study based on 1:1 semistructured interviews with EM consultants working full time in EDs across South West England. Eighteen EM consultants were interviewed across five EDs, the mean (SD) age of participants being 43. Read More

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A novel strategy for the rapid preparation and isolation of intact immune complexes from peptide mixtures.

J Mol Recognit 2014 Sep;27(9):566-74

Proteome Center Rostock, University Medicine Rostock, Rostock, Germany.

The development and application of a miniaturized affinity system for the preparation and release of intact immune complexes are demonstrated. Antibodies were reversibly affinity-adsorbed on pipette tips containing protein G´ and protein A, respectively. Antigen proteins were digested with proteases and peptide mixtures were exposed to attached antibodies; forming antibody-epitope complexes, that is, immune complexes. Read More

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September 2014

Biofilm-forming skin microflora bacteria are resistant to the bactericidal action of disinfectants used during blood donation.

Transfusion 2014 Nov 29;54(11):2974-82. Epub 2014 May 29.

Canadian Blood Services, Ottawa, Ontario, Canada.

Background: A one-step skin disinfection method containing 2% chlorhexidine-gluconate (CHG) and 70% isopropyl alcohol (IPA) is currently used by blood suppliers worldwide. Reports of bacterially contaminated platelet concentrates (PCs) indicate that skin disinfection is not fully effective. Approximately 20% of skin microflora exist as surface-attached aggregates (biofilms), known for displaying increased resistance to disinfectants. Read More

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November 2014

Phthalic acid chemical probes synthesized for protein-protein interaction analysis.

Int J Mol Sci 2013 Jun 24;14(7):12914-30. Epub 2013 Jun 24.

Department of Biotechnology, College of Life Science, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.

Plasticizers are additives that are used to increase the flexibility of plastic during manufacturing. However, in injection molding processes, plasticizers cannot be generated with monomers because they can peel off from the plastics into the surrounding environment, water, or food, or become attached to skin. Among the various plasticizers that are used, 1,2-benzenedicarboxylic acid (phthalic acid) is a typical precursor to generate phthalates. Read More

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Women's experiences of using drugs in weight management. An interpretative phenomenological analysis.

Appetite 2013 Jan 11;60(1):220-225. Epub 2012 Oct 11.

Department of Psychology, University of Wolverhampton, Wulfruna Street, Wolverhampton WV1 1LY, UK.

This study examines the experiences of women using drugs in weight management. Whilst some of the commonly used substances have been deemed to be safe, most, if not all, still have potentially dangerous side effects for the individual. Five women spoke of their experiences of using drugs in weight management through semi-structured interviews. Read More

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January 2013

Trypanosoma cruzi uses macropinocytosis as an additional entry pathway into mammalian host cell.

Microbes Infect 2012 Nov 23;14(14):1340-51. Epub 2012 Sep 23.

Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro - Av. Carlos Chagas Filho, 373, Bloco G - subsolo, Cidade Universitária, Ilha do Fundão, Rio de Janeiro 21941-902, Brazil.

Several intracellular pathogens are internalized by host cells via multiple endocytic pathways. It is no different with Trypanosoma cruzi. Evidences indicate that T. Read More

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November 2012

[IPA secretary and patron--Freud's patient, financial administrator and friend. Anton von Freund's letters to Sigmund Freud (1916-1919)].

Luzif Amor 2011 ;24(48):53-98

Although the letters, of which numerous and lengthy excerpts are presented in this paper, have repeatedly been used by scholars, they have so far remained unpublished. There are 45 items, written between 4. 1. Read More

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February 2012

Antibody detection employing sol-gel immobilized parasites.

J Immunol Methods 2008 Jun 27;335(1-2):65-70. Epub 2008 Mar 27.

Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Immunofluorescence assay (IFA) and immunoperoxidase assay (IPA) are useful diagnostic techniques for specific antibody detection for different diseases. Both involve several alternatives for immobilization of cells, such as solvent or heat fixation. Non-covalent immobilization implies rigorous storage conditions at -20 degrees C to preserve the slides, and usually numerous cells are detached during the washing steps, which can lead to inconsistencies in the results. Read More

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Health Technology Assessment of laparoscopic compared to conventional surgery with and without mesh for incisional hernia repair regarding safety, efficacy and cost-effectiveness.

GMS Health Technol Assess 2008 Mar 7;4:Doc01. Epub 2008 Mar 7.

Leibniz Universität Hannover, Forschungsstelle für Gesundheitsökonomie und Gesundheitssystemforschung, Hannover, Deutschland.

Introduction: Incisional hernias are a common complication following abdominal surgery and they represent about 80% of all ventral hernia. In uncomplicated postoperative follow-up they can develop in about eleven percent of cases and up to 23% of cases with wound infections or other forms of wound complications. Localisation and size of the incisional hernia can vary according to the causal abdominal scar. Read More

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Into the unknown: the anticipation and experience of membership of independent practitioner associations.

Authors:
Pauline Barnett

N Z Med J 2003 Apr 4;116(1171):U382. Epub 2003 Apr 4.

Department of Public Health and General Practice, Christchurch School of Medicine, Christchurch, New Zealand.

Aims: To understand why general practitioners (GPs) joined independent practitioner associations (IPAs), their concerns on joining, and the extent to which both positive and negative expectations have been realised.

Methods: A self-complete postal questionnaire to a sample of IPA rank-and-file members invited their views on their decision to join, their satisfaction with leadership, and the experience of being in an IPA.

Results: The most popular reasons for joining were related to the uncertainties of the health sector environment, including the prospect of contracting and the place of general practice within the health sector. Read More

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Usefulness of a small scale indirect 125I-labelled protein A binding assay for detection of monoclonal antibodies against hematopoietic cell surface antigens.

Leuk Res 1991 ;15(2-3):73-9

First Department of Internal Medicine, Kagawa Medical School, Japan.

We describe a small-scale solid-phase indirect 125iodine protein A binding assay (IPA) and discuss its usefulness for detection of hematopoietic cell surface antigens and for screening hybridoma clones producing monoclonal antibodies (McAbs) against K562 cell surface antigens correlated with differentiation induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA). This assay was performed in polystyrene Terasaki microtest plates instead of 96-well microtiter plates, using hematopoietic cells attached to the wells by air drying. This method facilitates washing procedures and reduces the radioactive waste. Read More

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