643,293 results match your criteria involved synthesis

The effects of Zinc-Methionine on glucose metabolism and insulin resistance during late pregnancy in ewes.

Domest Anim Endocrinol 2021 Jul 1;77:106647. Epub 2021 Jul 1.

Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

This study investigated the effects of dietary supplements of Zinc-Methionine (Zn-Met) on the zinc concentration of the blood and indices such as insulin resistance and glucose tolerance in late-pregnancy ewes to provide a practical solution to prevent metabolic diseases associated with energy status. In this research, 18 Makouei pregnant ewes were selected and randomized into 3 experimental groups: Group 1: Basal diet containing 10.3 mg Zn/kgDM and no zinc supplementation = CTR (Control); Group 2: Basal diet supplemented with low-dose zinc equivalent to the pregnancy requirements (30 mg Zn/kgDM) = LZN; Group 3: Basal diet supplemented with high-dose zinc (300 mg Zn/kgDM) = HZN. Read More

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Dynamic FDG-PET in localization of focal epilepsy: A pilot study.

Epilepsy Behav 2021 Jul 23;122:108204. Epub 2021 Jul 23.

Neurology, University of Virginia, Charlottesville, VA, USA. Electronic address:

Epilepsy surgery remains underutilized, in part because non-invasive methods of potential seizure foci localization are inadequate. We used high-resolution, parametric quantification from dynamic 2-[18F] fluoro-2-deoxy-d-glucose positron emission tomography (dFDG-PET) imaging to locate hypometabolic foci in patients whose standard clinical static PET images were normal. We obtained dFDG-PET brain images with simultaneous EEG in a one-hour acquisition on seven patients with no MRI evidence of focal epilepsy to record uptake and focal radiation decay. Read More

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Cellular and molecular regulation of the programmed death-1/programmed death ligand system and its role in multiple sclerosis and other autoimmune diseases.

J Autoimmun 2021 Jul 23;123:102702. Epub 2021 Jul 23.

Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile. Electronic address:

Programmed Cell Death 1 (PD-1) receptor and its ligands (PD-Ls) are essential to maintain peripheral immune tolerance and to avoid tissue damage. Consequently, altered gene or protein expression of this system of co-inhibitory molecules has been involved in the development of cancer and autoimmunity. Substantial progress has been achieved in the study of the PD-1/PD-Ls system in terms of regulatory mechanisms and therapy. Read More

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Verification of the Versatility of the In Vitro Enzymatic Reaction Giving (+)-cis-12-Oxo-phytodienoic Acid.

Bioorg Med Chem Lett 2021 Jul 23:128284. Epub 2021 Jul 23.

Laboratory of Natural Product Chemistry, Division of Fundamental AgriScience Research, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address:

Jasmonic acid (JA) is a plant hormone involved in the defense response against insects and fungi. JA is synthesized from α-linolenic acid (LA) by the octadecanoid pathway in plants. 12-oxo-Phytodienoic acid (OPDA) is one of the biosynthetic intermediates in this pathway. Read More

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TRIM15 promotes the invasion and metastasis of pancreatic cancer cells by mediating APOA1 ubiquitination and degradation.

Biochim Biophys Acta Mol Basis Dis 2021 Jul 23:166213. Epub 2021 Jul 23.

Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China; Sino-German Laboratory of Personalized Medicine for Pancreatic Cancer, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Most pancreatic ductal adenocarcinomas (PDACs) are diagnosed at an advanced or metastatic stage. Metastasis is the one of the major obstacles to prolonging the survival time of patients with pancreatic cancer. The tripartite motif (TRIM) family member TRIM15 has been implicated in cancer development. Read More

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Fatty acids and beyond: Age and Alzheimer's disease related changes in lipids reveal the neuro-nutraceutical potential of lipids in cognition.

Neurochem Int 2021 Jul 23:105143. Epub 2021 Jul 23.

The Florey Institute of Neuroscience and Mental Health, 30 Royal Parade, Parkville, Victoria, 3052, Australia; School of Biosciences, University of Melbourne, Parkville, Victoria, 3010, Australia. Electronic address:

Lipids are essential in maintaining brain function, and lipid profiles have been reported to be altered in aged and Alzheimer's disease (AD) brains as compared to healthy mature brains. Both age and AD share common metabolic hallmarks such as increased oxidative stress and perturbed metabolic function, and age remains the most strongly correlated risk factor for AD, a neurodegenerative disease. A major accompanying pathological symptom of these conditions is cognitive impairment, which is linked with changes in lipid metabolism. Read More

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Heparan sulfate promotes differentiation of white adipocytes to maintain insulin sensitivity and glucose homeostasis.

J Biol Chem 2021 Jul 23:101006. Epub 2021 Jul 23.

Department of Pharmacology, Tohoku University Graduate School of Medicine, 2-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan. Electronic address:

Heparan sulfate (HS), a highly sulfated linear polysaccharide, is involved in diverse biological functions in various tissues. Although previous studies have suggested a possible contribution of HS to the differentiation of white adipocytes, there has been no direct evidence supporting this. Here, we inhibited the synthesis of HS chains in 3T3-L1 cells using CRISPR/Cas9 technology, resulting in impaired differentiation of adipocytes with attenuated BMP4 (bone morphogenetic protein 4)/FGF1 (fibroblast growth factor 1) signaling pathways. Read More

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Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.

Microb Biotechnol 2021 Jul 26. Epub 2021 Jul 26.

College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.

Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and immunosuppressive activities. However, the yield of edeines in wild-type (WT) Brevibacillus is extremely low, and chemical synthesis of edeines is a time-consuming process. Genetic engineering has proven to be an effective approach to produce antibiotics with high yield. Read More

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Insufficient Iron in Floral Buds Impairs Pollen Development by Disrupting Tapetum Function.

Plant J 2021 Jul 26. Epub 2021 Jul 26.

Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 11529, Taiwan.

Reduction of crop yield due to iron (Fe) deficiency has always been a concern in agriculture. How insufficient Fe in floral buds affects pollen development remains unexplored. Here, plants transferred to Fe-deficient medium at the reproductive stage had reduced floral Fe content and viable pollen and showed a defective pollen outer wall, all restored by supplying floral buds with Fe. Read More

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New high-quality peach (Prunus persica L. Batsch) genome assembly to analyze the molecular evolutionary mechanism of volatile compounds in peach fruits.

Plant J 2021 Jul 26. Epub 2021 Jul 26.

The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Fruit Tree Breeding Technology), Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Peach (Prunus persica L. Batsch) is an economically important fruit crop worldwide. Although a high-quality peach genome has previously been published, Sanger sequencing was used for its assembly, which generated short contigs. Read More

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Apolipoprotein E deficiency activates thermogenesis of white adipose tissues in mice through enhancing β-hydroxybutyrate production from precursor cells.

FASEB J 2021 Aug;35(8):e21760

Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan.

White adipose tissue (WAT) has the capacity to undergo a white-to-beige phenotypic switch, known as browning, in response to stimuli such as cold. However, the mechanism underlying beige adipocyte formation is largely unknown. Apolipoprotein E (ApoE) is highly induced in WAT and has been implicated in lipid metabolism. Read More

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Tailored or adapted interventions for adults with chronic obstructive pulmonary disease and at least one other long-term condition: a mixed methods review.

Cochrane Database Syst Rev 2021 Jul 26;7:CD013384. Epub 2021 Jul 26.

Physiotherapy, Alfred Health, Melbourne, Australia.

Background: Chronic obstructive pulmonary disease (COPD) is a chronic respiratory condition characterised by shortness of breath, cough and recurrent exacerbations. People with COPD often live with one or more co-existing long-term health conditions (comorbidities). People with more severe COPD often have a higher number of comorbidities, putting them at greater risk of morbidity and mortality. Read More

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Predictive value of ERCC2, ABCC2 and MMP2 of response and long-term survival in locally advanced head and neck cancer patients treated with chemoradiotherapy.

Cancer Chemother Pharmacol 2021 Jul 26. Epub 2021 Jul 26.

Clinical Pharmacology Group, Health Research Institute of Santiago de Compostela (IDIS), Trav. Choupana s/n, 15706, Santiago, Spain.

Purpose: Genetic variants in genes involved in the distribution, metabolism, accumulation or repair of lesions are likely to influence the response of drugs used in the treatment of Head and Neck Cancer (HNC). We examine the effect of 36 SNPs on clinical outcomes in patients with locally advanced HNC who were receiving platinum-based chemoradiotherapy (CRT).

Methods: These SNPs were genotyped in 110 patients using the iPLEX Gold assay on the MassARRAY method in blood DNA samples and used Kaplan-Meier and Cox regression analyses to compare genotype groups with the survival. Read More

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Metabolomic biomarkers of low BMD: a systematic review.

Osteoporos Int 2021 Jul 26. Epub 2021 Jul 26.

Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Due to the metabolic nature of osteoporosis, this study was conducted to identify metabolomic studies investigating the metabolic profile of low bone mineral density (BMD) and osteoporosis. A comprehensive systematic literature search was conducted through PubMed, Web of Science, Scopus, and Embase databases up to April 08, 2020, to identify observational studies with cross-sectional or case-control designs investigating the metabolic profile of low BMD in adults using biofluid specimen via metabolomic platform. The quality assessment panel specified for the "omics"-based diagnostic research (QUADOMICS) tool was used to estimate the methodologic quality of the included studies. Read More

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The role of mTOR in age-related diseases.

J Enzyme Inhib Med Chem 2021 Dec;36(1):1679-1693

Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czechia.

The ageing population is becoming a significant socio-economic issue. To address the expanding health gap, it is important to deepen our understanding of the mechanisms underlying ageing in various organisms at the single-cell level. The discovery of the antifungal, immunosuppressive, and anticancer drug rapamycin, which possesses the ability to extend the lifespan of several species, has prompted extensive research in the areas of cell metabolic regulation, development, and senescence. Read More

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December 2021

Acetate Promotes Lipogenesis in Adipocytes but not in Hepatocytes of Chickens.

Br Poult Sci 2021 Jul 26. Epub 2021 Jul 26.

College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.

The role of acetate in lipogenesis of chickens remains largely unknown. This trial investigated the effect of sodium acetate (SA) on chicken fat metabolism and experiments.The results indicated that supplementation of SA (1. Read More

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[Diversity and PICRUSt2-based Predicted Functional Analysis of Bacterial Communities During the Start-up of ANAMMOX].

Huan Jing Ke Xue 2021 Aug;42(8):3875-3885

Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330096, China.

Bacterial communities are vital for efficient nitrogen removal in an anaerobic ammonium oxidation (ANAMMOX) system. However, the diversity and functional characteristics of a bacterial community during the start-up of ANAMMOX has not been reported. In this study, an up-flow anaerobic sludge bed reactor was used to start-up the ANAMMOX system, and 16S rRNA high-throughput gene sequencing, combined with PICRUSt2-based functional prediction analysis, was used to investigate the dynamic changes in diversity and function of the bacterial community at different times (d0, d30, d60, and d90) during the start-up. Read More

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[Gene expression profiles of myopic mouse scleral fibroblasts: a bioinformatics analysis based on single-cell RNA sequencing].

J Yu Y Mo

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jul;41(7):1087-1092

Department of Ophthalmology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.

Objective: To investigate the changes in gene expression profiles of mouse scleral fibroblasts after myopia using single-cell RNA sequencing technology and explore the mechanism of dysfunction of the scleral fibroblasts in myopia.

Methods: Normal healthy C57BL/6J mice were randomly divided into negative control group and myopia model group (=6), and in the latter group, form deprivation myopia was induced in the right eye using translucent goggles. Single cell capture was performed in the right eye to obtain the scleral fibroblasts for RNA sequencing. Read More

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Placental insufficiency induces a sexually dimorphic response in the expression of cardiac growth and metabolic signalling molecules upon exposure to a postnatal western diet in guinea pigs.

J Dev Orig Health Dis 2021 Jul 26:1-13. Epub 2021 Jul 26.

Early Origins of Adult Health Research Group, Health and Biomedical Innovation, UniSA: Clinical and Health Sciences, University of South Australia, Adelaide, SA, Australia.

There is a strong relationship between low birth weight (LBW) and an increased risk of developing cardiovascular disease (CVD). In postnatal life, LBW offspring are becoming more commonly exposed to the additional independent CVD risk factors, such as an obesogenic diet. However, how an already detrimentally programmed LBW myocardium responds to a secondary insult, such as an obesogenic diet (western diet; WD), during postnatal life is ill defined. Read More

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A quinoxaline-based compound ameliorates bone loss in ovariectomized mice.

Exp Biol Med (Maywood) 2021 Jul 25:15353702211032133. Epub 2021 Jul 25.

Department of Basic Medicine, Xi'an Medical University, Xi'an 710021, PR China.

DMB (6,7-dichloro-2-methylsulfonyl-3-Ntert-butylaminoquinoxaline) is a quinoxaline-based compound that has been investigated as a glucagon-like peptide-1 receptor (GLP-1R) agonist. To clarify anti-osteoporosis effect of DMB, an osteoporotic mice model was established by ovariectomy (OVX) operation. The OVX mice were given intraperitoneally DMB, exendin-4 (EX-4), or 17β-estradiol (E) for two months. Read More

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Preparation of a Gangue-Based X-type Zeolite Molecular Sieve as a Multiphase Fenton Catalyst and Its Catalytic Performance.

ACS Omega 2021 Jul 2;6(28):18414-18425. Epub 2021 Jul 2.

Inner Mongolia Key Laboratory of Environmental Chemistry, College of Chemistry and Environmental Science, Inner Mongolia Normal University, Hohhot 010022, China.

In this study, a series of X-type zeolite molecular sieve catalysts, modified with copper (Cu-X), were prepared by an alkali fusion-hydrothermal synthesis using coal gangue from Inner Mongolia. These catalysts were used in the degradation of the methylene blue dye by a Fenton-like reaction. Characterization results showed that Cu is considered to be present in the surface structure of the zeolite in the form of doped Cu ions and metal oxide. Read More

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Pickering Emulsions Based on the pH-Responsive Assembly of Food-Grade Chitosan.

ACS Omega 2021 Jul 7;6(28):17915-17922. Epub 2021 Jul 7.

State-Key Laboratory of Chemical Engineering and Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

Few natural, biocompatible, and inexpensive emulsifiers are available because such emulsifiers must satisfy severe requirements, be produced synthetically rather than naturally, be nontoxic, and require minimal effort to produce. Therefore, the synthesis of food-grade and biocompatible nanoparticles as an alternative to surfactants has recently received attention in the industry. However, many previous efforts involved chemical modification of materials or the introduction of secondary cocomponents for emulsion formation. Read More

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Structural and Functional Elucidation of IF-3 Protein of Involved in Protein Biosynthesis: An Approach.

Biomed Res Int 2021 1;2021:9050026. Epub 2021 Jul 1.

School of Psychiatry, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia.

is a thermophilic bacterium that produces a multitude of proteins within its genome. Bioinformatics strategies can facilitate comprehending this organism through functional and structural interpretation assessments. This study is aimed at allocating the structure and function through an approach required for bacterial protein biosynthesis. Read More

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Variations in Microbial Diversity and Metabolite Profiles of Female Landrace Finishing Pigs With Distinct Feed Efficiency.

Front Vet Sci 2021 9;8:702931. Epub 2021 Jul 9.

Animal Science and Technology College, Beijing University of Agriculture, Beijing, China.

To enhance pig production, feed efficiency (FE) should be improved; however, the mechanisms by which gut microbes affect FE in pigs have not been fully elucidated. To investigate the differences between the composition and functionality of the gut microbiota associated with low and high FE, microbial compositions were characterized using 16S rRNA sequencing, functional annotations were performed by shotgun metagenomics, and metabolomic profiles were created by GC-TOF-MS from female Landrace finishing pigs with low and high feed conversion ratios (FCRs). was enriched in the gut microbiota of individuals with low FCRs (and thus high FE), while abundance was significantly higher in individuals with high FCRs (and thus low FE). Read More

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Construction and Analysis of Coexpression Network to Understand Biological Responses in Chickens Infected by .

Front Vet Sci 2021 9;8:688684. Epub 2021 Jul 9.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

Coccidiosis, caused by various species, is a major parasitic disease in chickens. Our understanding of how chickens respond to coccidian infections is highly limited at both the molecular and cellular levels. In this study, coexpression modules were identified by weighted gene coexpression network analysis in chickens infected with . Read More

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Vagus Nerve Stimulation Promotes Epithelial Proliferation and Controls Colon Monocyte Infiltration During DSS-Induced Colitis.

Front Med (Lausanne) 2021 7;8:694268. Epub 2021 Jul 7.

Translational Research Center for Gastrointestinal Disorders (TARGID), Lab for Intestinal Neuro-Immune Interaction, Department of Chronic Diseases, Metabolism and Ageing, Katholieke Universiteit Leuven - University of Leuven, Leuven, Belgium.

We previously showed increased susceptibility to dextran sulfate sodium (DSS)-induced colitis in vagotomized mice. Here, we evaluated whether vagus nerve stimulation (VNS) is able to reduce the severity of DSS colitis and aimed to unravel the mechanism involved. Colitis was induced in wild type mice by 2. Read More

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Accessing Mitochondrial Protein Import in Living Cells by Protein Microinjection.

Front Cell Dev Biol 2021 7;9:698658. Epub 2021 Jul 7.

Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia.

Mitochondrial protein biogenesis relies almost exclusively on the expression of nuclear-encoded polypeptides. The current model postulates that most of these proteins have to be delivered to their final mitochondrial destination after their synthesis in the cytoplasm. However, the knowledge of this process remains limited due to the absence of proper experimental real-time approaches to study mitochondria in their native cellular environment. Read More

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GLP-1-Induced AMPK Activation Inhibits PARP-1 and Promotes LXR-Mediated ABCA1 Expression to Protect Pancreatic β-Cells Against Cholesterol-Induced Toxicity Through Cholesterol Efflux.

Front Cell Dev Biol 2021 7;9:646113. Epub 2021 Jul 7.

Department of Gastrointestinal Surgery, The Third Xiangya Hospital, Central South University, Changsha, China.

T2DM (Type 2 diabetes) is a complex, chronic disease characterized as insulin resistance and islet β-cell dysfunction. Bariatric surgeries such as Roux-en-Y gastric bypass (RYGB) surgery and laparoscopic sleeve gastrectomy (LSG) have become part of a critical treatment regimen in the treatment of obesity and T2DM. Moreover, GLP-1 increase following bariatric surgery has been regarded as a significant event in bariatric surgery-induced remission of T2DM. Read More

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Gold-Catalyzed Complementary Nitroalkyne Internal Redox Process: A DFT Study.

Front Chem 2021 9;9:689780. Epub 2021 Jul 9.

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.

Gold-catalysis, in this century, is one of the most emerging and promising new areas of research in organic synthesis. During the last two decades, a wide range of distinct synthetic methodologies have been unveiled employing homogeneous gold catalysis and aptly applied in the synthesis of numerous natural products and biologically active molecules. Among these, the reactions involving α-oxo gold carbene/α-imino gold carbene intermediates are of contemporary interest, in view of their synthetic potential and also due to the need to understand the bonding involved in these complexes. Read More

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Transcription Factors and Methylation Drive Prognostic miRNA Dysregulation in Hepatocellular Carcinoma.

Front Oncol 2021 1;11:691115. Epub 2021 Jul 1.

Laboratory for Comparative Genomics and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China.

Many dysregulated microRNAs (miRNAs) have been suggested to serve as oncogenes or tumor suppressors to act as diagnostic and prognostic factors for HCC patients. However, the dysregulated mechanisms of miRNAs in HCC remain largely unknown. Herein, we firstly identify 114 disordered mature miRNAs in HCC, 93 of them are caused by dysregulated transcription factors, and 10 of them are driven by the DNA methylation of their promoter regions. Read More

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