19,770 results match your criteria involved -atpase

Inoculation with Bacillus amyloliquefaciens and mycorrhiza confers tolerance to drought stress and improve seed yield and quality of soybean plant.

Physiol Plant 2021 May 8. Epub 2021 May 8.

Department of Agricultural Microbiology, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.

The present study aimed to evaluate the effect of Bacillus amyloliquefaciens and/or Arbuscular Mycorrhizal Fungi (AMF) as natural biofertilizers on biomass, yield and seed nutritive quality of soybean (Giza 111). The conditions investigated include a well-watered (WW) control and irrigation withholding at the seed development stage (R5, after 90 days from sowing) (DS). Co-inoculation with B. Read More

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Comparative Proteomic Analysis of Sweet Orange Petiole Provides Insights Into the Development of Huanglongbing Symptoms.

Front Plant Sci 2021 19;12:656997. Epub 2021 Apr 19.

The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Huanglongbing (HLB) is the most destructive citrus disease worldwide. This is associated with the phloem-limited bacterium Liberibacter, and the typical symptom is leaf blotchy mottle. To better understand the biological processes involved in the establishment of HLB disease symptoms, the comparative proteomic analysis was performed to reveal the global protein accumulation profiles in leaf petiole, where there are massive HLB pathogens of L. Read More

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Construction of a SUMOylation regulator-based prognostic model in low-grade glioma.

J Cell Mol Med 2021 May 5. Epub 2021 May 5.

Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, China.

Low-grade glioma (LGG) is an intracranial malignant tumour that mainly originates from astrocytes and oligodendrocytes. SUMOylation is one of the post-translational modifications but studies of SUMOylation in LGG is quite limited. Transcriptome data, single nucleotide variant (SNV) data and clinical data of LGG were derived from public databases. Read More

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Ciliary Ca2+ pumps regulate intraciliary Ca2+ from the action potential and may co-localize with ciliary voltage-gated Ca2+ channels.

J Exp Biol 2021 May 4;224(9). Epub 2021 May 4.

Department of Biology, University of Vermont, Burlington, VT 05405, USA.

Calcium ions (Ca2+) entering cilia through the ciliary voltage-gated calcium channels (CaV) during the action potential causes reversal of the ciliary power stroke and backward swimming in Paramecium tetraurelia. How calcium is returned to the resting level is not yet clear. Our focus is on calcium pumps as a possible mechanism. Read More

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Deletion of Cdh-16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse.

Am J Physiol Renal Physiol 2021 May 3. Epub 2021 May 3.

Department of Medicine/Nephrology, Yale School of Medicine, United States.

Ksp-cadherin (Cadherin-16) is an atypical member of the cadherin superfamily of cell adhesion molecules that is ubiquitously expressed on the basolateral membrane of epithelial cells lining the nephron and the collecting system of the mammalian kidney. The principal aim of the present study was to determine if Ksp-cadherin played a critical role in the development and maintenance of the adult mammalian kidney by generating and evaluating a mouse line deficient in Ksp-cadherin. Ksp-null mutant animals were viable and fertile, and kidneys from both neonates and adults showed no evidence of structural abnormalities. Read More

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NFAT5 Is Involved in GRP-Enhanced Secretion of GLP-1 by Sodium.

Int J Mol Sci 2021 Apr 12;22(8). Epub 2021 Apr 12.

Division of Renal Diseases & Hypertension, The George Washington University School of Medicine & Health Science, Washington, DC 20052, USA.

Gastrin, secreted by G-cells, and glucagon-like peptide-1 (GLP-1), secreted by L-cells, may participate in the regulation of sodium balance. We studied the effect of sodium in mice in vivo and mouse ileum and human L-cells, on GLP-1 secretion, and the role of NFAT5 and gastrin-releasing peptide receptor (GRPR) in this process. A high-sodium diet increases serum GLP-1 levels in mice. Read More

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Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome.

Front Mol Neurosci 2021 12;14:613161. Epub 2021 Apr 12.

Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Centre National de la Recherche Scientifique, Strasbourg, France.

The cerebellum harbors a circadian clock that can be shifted by scheduled mealtime and participates in behavioral anticipation of food access. Large-scale two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry was used to identify day-night variations in the cerebellar proteome of mice fed either during daytime or nighttime. Experimental conditions led to modified expression of 89 cerebellar proteins contained in 63 protein spots. Read More

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Structural insight into mutations at 155 position of valosin containing protein (VCP) linked to inclusion body myopathy with Paget disease of bone and frontotemporal Dementia.

Saudi J Biol Sci 2021 Apr 19;28(4):2128-2138. Epub 2021 Feb 19.

Department of Neurology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, China.

Mutations in have been implicated in the pathology linked to inclusion body myopathy, paget disease of bone and frontotemporal dementia (IBMPFD). VCP is an essential component of AAA-ATPase superfamily involved in various cellular functions. Advanced analysis was performed using prediction based servers to determine the most deleterious mutation. Read More

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Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion.

PLoS One 2021 28;16(4):e0250296. Epub 2021 Apr 28.

Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas, United States of America.

Salmonella Enteritidis is an intracellular foodborne pathogen that has developed multiple mechanisms to alter poultry intestinal physiology and infect the gut. Short chain fatty acid butyrate is derived from microbiota metabolic activities, and it maintains gut homeostasis. There is limited understanding on the interaction between S. Read More

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Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway.

Int J Mol Med 2021 Jun 28;47(6). Epub 2021 Apr 28.

Department of Cardiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.

Naringin, a natural bioflavonoid, has been shown to exert protective effects in multiple cardiovascular diseases; however, the protective effects of naringin against hypoxic/ischemia‑induced myocardial are not yet fully understood. Autophagy is a vital factor involved in the pathogenesis of myocardial injury. The aim of the present study was to investigate the protective effects of naringin on H9c2 cells against chemical hypoxia [cobalt chloride (CoCl)]‑induced injury. Read More

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Molecular basis of ventricular arrhythmogenicity in a Pgc-1α deficient murine model.

Mol Genet Metab Rep 2021 Jun 9;27:100753. Epub 2021 Apr 9.

Faculty of Health and Medical Sciences, University of Surrey, GU2 7AL Guildford, United Kingdom.

Mitochondrial dysfunction underlying metabolic disorders such as obesity and diabetes mellitus is strongly associated with cardiac arrhythmias. Murine Pgc-1α hearts replicate disrupted mitochondrial function and model the associated pro-arrhythmic electrophysiological abnormalities. Quantitative PCR, western blotting and histological analysis were used to investigate the molecular basis of the electrophysiological changes associated with mitochondrial dysfunction. Read More

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Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli.

Nat Commun 2021 04 23;12(1):2405. Epub 2021 Apr 23.

Indiana University School of Medicine, Department of Pediatrics, Division of Nephrology, Indianapolis, IN, USA.

Kidney intercalated cells are involved in acid-base homeostasis via vacuolar ATPase expression. Here we report six human intercalated cell subtypes, including hybrid principal-intercalated cells identified from single cell transcriptomics. Phagosome maturation is a biological process that increases in biological pathway analysis rank following exposure to uropathogenic Escherichia coli in two of the intercalated cell subtypes. Read More

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Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.

Cell Tissue Res 2021 Apr 22. Epub 2021 Apr 22.

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, CAL, T2N 4N1, Canada.

The sperm-derived oocyte activating factor, phospholipase C zeta (PLC ζ), is the only PLC isoform reported in cattle. The objectives were to (1) localize PLC ζ in fresh and capacitated bovine sperm and (2) investigate the activation of PLC ζ during bull sperm capacitation and contributions of PLC activity to this process. We confirmed interaction of testis-specific isoform of Na/K-ATPase (ATP1A4) with PLC ζ (immunolocalization and immunoprecipitation) and tyrosine phosphorylation (immunoprecipitation) of PLC ζ (a post-translational protein modification commonly involved in activation of PLC in somatic cells) during capacitation. Read More

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Identification of mutations in the ATP7B gene in 14 Wilson disease children: Case series.

Medicine (Baltimore) 2021 Apr;100(16):e25463

Department of Neurology.

Introduction: Wilson Disease (WD) is an autosomal recessive inherited metabolic disease caused by mutations in the ATPase copper transporting beta gene (ATP7B). WD can cause fatal neurological and hepatic disorders if not diagnosed and treated.

Objective: To analyze the disease-causing mutations of 14 Chinese WD children, 11 of whom are diagnosed with hepatic disorders, 2 with neurological degeneration and 1 with both hepatic and neurological disorders. Read More

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AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.

Eur J Med Chem 2021 Apr 10;219:113446. Epub 2021 Apr 10.

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, United States. Electronic address:

ATPases Associated with Diverse Cellular Activity (AAA ATPase) are essential enzymes found in all organisms. They are involved in various processes such as DNA replication, protein degradation, membrane fusion, microtubule serving, peroxisome biogenesis, signal transduction, and the regulation of gene expression. Due to the importance of AAA ATPases, several researchers identified and developed small-molecule inhibitors against these enzymes. Read More

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[MutL Protein from the Neisseria gonorrhoeae Mismatch Repair System: Interaction with ATP and DNA].

Mol Biol (Mosk) 2021 Mar-Apr;55(2):289-304

Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119991 Russia.

The mismatch repair system (MMR) ensures the stability of genetic information during DNA replication in almost all organisms. Mismatch repair is initiated after recognition of a non-canonical nucleotide pair by the MutS protein and the formation of a complex between MutS and MutL. Eukaryotic and most bacterial MutL homologs function as endonucleases that introduce a single-strand break in the daughter strand of the DNA, thus activating the repair process. Read More

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Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering.

Front Cell Dev Biol 2021 1;9:636553. Epub 2021 Apr 1.

Department of Cardiology, The First Medical Center of PLA General Hospital, Beijing, China.

Our previous research has shown that type-2a Sarco/endoplasmic reticulum Ca-ATPase (SERCA2a) undergoes posttranscriptional oxidative modifications in cardiac microvascular endothelial cells (CMECs) in the context of excessive cardiac oxidative injury. However, whether SERCA2a inactivity induces cytosolic Ca imbalance in mitochondrial homeostasis is far from clear. Mitofusin2 (Mfn2) is well known as an important protein involved in endoplasmic reticulum (ER)/mitochondrial Ca tethering and the regulation of mitochondrial quality. Read More

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New insights on human Hsp70-escort protein 1: Chaperone activity, interaction with liposomes, cellular localizations and HSPA's self-assemblies remodeling.

Int J Biol Macromol 2021 Apr 16;182:772-784. Epub 2021 Apr 16.

Sao Carlos Institute of Chemistry, University of Sao Paulo, Sao Carlos, SP, Brazil. Electronic address:

The 70 kDa heat shock proteins (Hsp70) are prone to self-assembly under thermal stress conditions, forming supramolecular assemblies (SMA), what may have detrimental consequences for cellular viability. In mitochondria, the cochaperone Hsp70-escort protein 1 (Hep1) maintains mitochondrial Hsp70 (mtHsp70) in a soluble and functional state, contributing to preserving proteostasis. Here we investigated the interaction between human Hep1 (hHep1) and HSPA9 (human mtHsp70) or HSPA1A (Hsp70-1A) in monomeric and thermic SMA states to unveil further information about the involved mechanisms. Read More

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Synergistic Inhibition of SARS-CoV-2 Replication Using Disulfiram/Ebselen and Remdesivir.

ACS Pharmacol Transl Sci 2021 Apr 26;4(2):898-907. Epub 2021 Mar 26.

Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.

The SARS-CoV-2 replication and transcription complex (RTC) comprising nonstructural protein (nsp) 2-16 plays crucial roles in viral replication, reducing the efficacy of broad-spectrum nucleoside analog drugs such as remdesivir and evading innate immune responses. Most studies target a specific viral component of the RTC such as the main protease or the RNA-dependent RNA polymerase. In contrast, our strategy is to target conserved domains of the RTC to prevent SARS-CoV-2 genome replication and to create a high barrier to viral resistance and/or evasion of antiviral drugs. Read More

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Identify Candidate Genes in the Interaction between Abdominal Aortic Aneurysm and Type 2 Diabetes Mellitus by Using Biomedical Discovery Support System.

Chin Med Sci J 2021 Mar;36(1):50-56

Department of Vascular Surgery, Tian Jin First Center Hospital, Tianjin 300192, China.

Objective To explore the candidate genes that play significant roles in the interconnection between abdominal aortic aneurysm (AAA) and type 2 diabetes mellitus (DM). Methods We used the Biomedical Discovery Support System (BITOLA) to screen out the candidate intermediate molecular (CIM) "Gene or Gene Product" that are related to AAA and DM. The dataset of GSE13760, GSE7084, GSE57691, GSE47472 were used to analyze the differentially expressed genes (DEGs) of AAA and DM compared to the healthy status. Read More

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A positive charge region of Salmonella FliI is required for ATPase formation and efficient flagellar protein export.

Commun Biol 2021 Apr 12;4(1):464. Epub 2021 Apr 12.

Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.

The FliHFliI complex is thought to pilot flagellar subunit proteins from the cytoplasm to the transmembrane export gate complex for flagellar assembly in Salmonella enterica. FliI also forms a homo-hexamer to hydrolyze ATP, thereby activating the export gate complex to become an active protein transporter. However, it remains unknown how this activation occurs. Read More

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Functional interpretation of ATAD3A variants in neuro-mitochondrial phenotypes.

Genome Med 2021 Apr 12;13(1):55. Epub 2021 Apr 12.

Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.

Background: ATPase family AAA-domain containing protein 3A (ATAD3A) is a nuclear-encoded mitochondrial membrane-anchored protein involved in diverse processes including mitochondrial dynamics, mitochondrial DNA organization, and cholesterol metabolism. Biallelic deletions (null), recessive missense variants (hypomorph), and heterozygous missense variants or duplications (antimorph) in ATAD3A lead to neurological syndromes in humans.

Methods: To expand the mutational spectrum of ATAD3A variants and to provide functional interpretation of missense alleles in trans to deletion alleles, we performed exome sequencing for identification of single nucleotide variants (SNVs) and copy number variants (CNVs) in ATAD3A in individuals with neurological and mitochondrial phenotypes. Read More

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Structural insight into phospholipid transport by the MlaFEBD complex from P. aeruginosa.

J Mol Biol 2021 Apr 9:166986. Epub 2021 Apr 9.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing100101, China. Electronic address:

The outer membrane (OM) of Gram-negative bacteria, which consists of lipopolysaccharides (LPS) in the outer leaflet and phospholipids (PLs) in the inner leaflet, plays a key role in antibiotic resistance and pathogen virulence. The maintenance of lipid asymmetry (Mla) pathway is known to be involved in PL transport and contributes to the lipid homeostasis of the OM, yet the underlying molecular mechanism and the directionality of PL transport in this pathway remain elusive. Here, we reported the cryo-EM structures of the ATP-binding cassette (ABC) transporter MlaFEBD from P. Read More

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Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H-ATPase Activity and Gene Expression.

Front Plant Sci 2021 25;12:659694. Epub 2021 Mar 25.

Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czechia.

A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, although it is unclear whether host H-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), , on H-ATPase activity, and gene expression of trifoliate orange () seedlings subjected to well-watered (WW) and drought stress (DS), together with the changes in leaf gas exchange, root morphology, soil pH value, and ammonium content. Soil drought treatment dramatically increased H-ATPase activity of leaf and root, and AMF inoculation further strengthened the increased effect. Read More

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Effect of Dynamic and Static Load on the Concentration of Myokines in the Blood Plasma and Content of Sodium and Potassium in Mouse Skeletal Muscles.

Biochemistry (Mosc) 2021 Mar;86(3):370-381

National Research Tomsk State University, Tomsk, 634050, Russia.

Modulation of cytokine production by physical activity is of considerable interest, since it might be a promising strategy for correcting metabolic processes at both cellular and systemic levels. The content of IL-6, IL-8, and IL-15 in the plasma and the concentration of monovalent cations in the skeletal muscles of trained and untrained mice were studied at different periods after static and dynamic exercises. Dynamic loads caused an increase in the IL-6 content and decrease in the IL-15 content in the plasma of untrained mice, but produced no effect on the concentration of IL-8. Read More

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Exploiting pyocyanin to treat mitochondrial disease due to respiratory complex III dysfunction.

Nat Commun 2021 04 8;12(1):2103. Epub 2021 Apr 8.

Department of Biology, University of Padova, Padova, Italy.

Mitochondrial diseases impair oxidative phosphorylation and ATP production, while effective treatment is still lacking. Defective complex III is associated with a highly variable clinical spectrum. We show that pyocyanin, a bacterial redox cycler, can replace the redox functions of complex III, acting as an electron shunt. Read More

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Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle.

J Virol 2021 Apr 7. Epub 2021 Apr 7.

Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA.

The classical swine fever virus (CSFV) glycoprotein E2 is the major structural component of the virus particle. E2 is involved in several functions such as virus adsorption to the cell, the elicitation of protective immune responses, and virus virulence in swine. Using a yeast two-hybrid system, we previously identified the swine host protein Torsin-1A, an ATPase protein residing in the endoplasmic reticulum and inner nucleus membrane of the cell, as a specific binding partner for E2. Read More

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Disposition of Proteins and Lipids in Synaptic Membrane Compartments Is Altered in Q175/Q7 Huntington's Disease Mouse Striatum.

Front Synaptic Neurosci 2021 18;13:618391. Epub 2021 Mar 18.

Department of Neurology, Massachusetts General Hospital, Boston, MA, United States.

Dysfunction at synapses is thought to be an early change contributing to cognitive, psychiatric and motor disturbances in Huntington's disease (HD). In neurons, mutant Huntingtin collects in aggregates and distributes to the same sites as wild-type Huntingtin including on membranes and in synapses. In this study, we investigated the biochemical integrity of synapses in HD mouse striatum. Read More

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HBx-upregulated MAFG-AS1 promotes cell proliferation and migration of hepatoma cells by enhancing MAFG expression and stabilizing nonmuscle myosin IIA.

FASEB J 2021 May;35(5):e21529

Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China.

To identify hepatitis B virus (HBV)-related lncRNA(s), we previously examined the transcription profiles of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells by RNA deep sequencing and identified 38 upregulated long noncoding RNAs (lncRNAs). In the present study, the lncRNA MAFG-AS1 is investigated in detail because its gene is located adjacent to the MAFG gene, which is an important transcription factor involved in cell proliferation. The level of MAFG-AS1 is significantly higher in HCC tissue than in nontumor tissues. Read More

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Defects in KCNJ16 Cause a Novel Tubulopathy with Hypokalemia, Salt Wasting, Disturbed Acid-Base Homeostasis, and Sensorineural Deafness.

J Am Soc Nephrol 2021 Apr 2. Epub 2021 Apr 2.

Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.

Background: The transepithelial transport of electrolytes, solutes, and water in the kidney is a well-orchestrated process involving numerous membrane transport systems. Basolateral potassium channels in tubular cells not only mediate potassium recycling for proper Na,K-ATPase function but are also involved in potassium and pH sensing. Genetic defects in cause EAST/SeSAME syndrome, characterized by renal salt wasting with hypokalemic alkalosis associated with epilepsy, ataxia, and sensorineural deafness. Read More

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