1,307,555 results match your criteria involved

Statistical Development and Validation of Clinical Prediction Models.

Anesthesiology 2021 Jul 30. Epub 2021 Jul 30.

Summary: Clinical prediction models in anesthesia and surgery research have many clinical applications including preoperative risk stratification with implications for clinical utility in decision-making, resource utilization, and costs. It is imperative that predictive algorithms and multivariable models are validated in a suitable and comprehensive way in order to establish the robustness of the model in terms of accuracy, predictive ability, reliability, and generalizability. The purpose of this article is to educate anesthesia researchers at an introductory level on important statistical concepts involved with development and validation of multivariable prediction models for a binary outcome. Read More

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Impacts of HfZrO thickness and anneal temperature on performance of MoS2 negative-capacitance field-effect transistors.

Nanotechnology 2021 Jul 30. Epub 2021 Jul 30.

School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Wuhan, 430074, CHINA.

The MoS2 negative-capacitance field-effect transistor (NCFET) with the ultra-thin (3 nm) HfZrO (HZO) as NC layer and 2-nm Al2O3 as dielectric layer is successfully fabricated by optimizing the annealing temperature and the HZO thickness. Excellent subthreshold swing (SS = 33.5 mV/dec) is achieved, with an on/off current ratio of 1. Read More

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Comparison of aesthetic outcomes between open and endoscopically treated sagittal craniosynostosis.

J Neurosurg Pediatr 2021 Jul 30:1-7. Epub 2021 Jul 30.

Departments of1Neurological Surgery and.

Objective: In the last several decades, there has been much debate regarding the ideal treatment for sagittal synostosis. The purpose of this study was to compare perioperative, anthropometric, and subjective assessments of cosmetic outcomes between open and endoscopic management of isolated sagittal synostosis.

Methods: At their routine postoperative follow-up, pediatric patients with sagittal craniosynostosis were recruited to undergo digital cranial measurement and standardized photography for objective and subjective assessments of perioperative outcomes. Read More

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Lithium effects on Hippocampus volumes in patients with bipolar disorder.

J Affect Disord 2021 Jul 18;294:521-526. Epub 2021 Jul 18.

Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy; Department of Neurosciences and Mental Health, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. Electronic address:

Background: Lithium is one of the most effective medications for bipolar disorder episode prevention, but its mechanism of action is still largely unknown. The hippocampus is a subcortical cerebral structure involved in the formation of emotional responses, cognition and various primitive functions, altered during affective episodes. Deviations in the anatomy or physiology of the hippocampus would partially explain the symptomatology of bipolar subjects, and restoration may reflect treatment response. Read More

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Long-term low-dose oxytetracycline potentially leads to neurobehavioural changes.

Ecotoxicol Environ Saf 2021 Jul 27;223:112546. Epub 2021 Jul 27.

Department of Infectious Disease, Xinhua Children's Hospital, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China. Electronic address:

Trace levels of oxytetracycline (OTC)-a veterinary antibiotic and feed additive-are widespread in the environment. Studies revealed that OTC potentially impairs thyroid function, which may affect neurobehaviour; however, the impact of exposure to environmental concentrations of OTC on adult neurobehaviour is unknown. In this study, the effects of OTC on zebrafish after 30-day exposure were investigated. Read More

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Simultaneous determination of tetra-, penta- and hexachlorobutadienes in shellfish by gas chromatography-triple quadrupole mass spectrometry.

Environ Pollut 2021 Jul 24;289:117845. Epub 2021 Jul 24.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310000, China.

Polychloro-1,3-butadienes (polyCBDs) are attracting increasing concern due to their high toxicity. However, research on multiple polyCBDs in aquatic biota is still extremely limited. In this study, a sensitive method for simultaneous determination of nine polyCBD (Cl-Cl) congeners, including six tetrachlorobutadiene (TeCBD) isomers, two pentachlorobutadiene (PeCBD) isomers, and hexachlorobutadiene (HCBD), in shellfish was developed based on accelerated solvent extraction (ASE), solid-phase extraction (SPE) clean-up and gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS/MS). Read More

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Aging induces abnormal accumulation of Aβ in extracellular vesicle and/or intraluminal membrane vesicle-rich fractions in nonhuman primate brain.

Neurobiol Aging 2021 Jul 5;106:268-281. Epub 2021 Jul 5.

Section of Cell Biology and Pathology, Department of Alzheimer's Disease Research, Center for Development of Advanced Medicine for Dementia, Obu, Aichi, Japan; Laboratory of Experimental Animals, Research and Development Management Center, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan; Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba, Ibaraki, Japan. Electronic address:

Aβ metabolism in the brain is mediated by endocytosis, one part of the intracellular membrane trafficking system. We previously showed that aging attenuates the interaction of dynein with dynactin, which disrupts the endosomal/lysosomal trafficking pathway involved in Aβ metabolism, resulting in intracellular accumulation of Aβ. Several studies have shown that in Alzheimer's disease (AD), intraneuronal accumulation of Aβ precedes extracellular Aβ depositions. Read More

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A combinatorial drug screen in PDX-derived primary rhabdomyosarcoma cells identifies the NOXA - BCL-XL/MCL-1 balance as target for re-sensitization to first-line therapy in recurrent tumors.

Neoplasia 2021 Jul 27;23(9):929-938. Epub 2021 Jul 27.

Department of Oncology and Children's Research Center, University Children's Hospital, Zurich, Switzerland. Electronic address:

First-line therapy for most pediatric sarcoma is based on chemotherapy in combination with radiotherapy and surgery. A significant number of patients experience drug resistance and development of relapsed tumors. Drugs that have the potential to re-sensitize relapsed tumor cells toward chemotherapy treatment are therefore of great clinical interest. Read More

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Biomarker-based assessment of sublethal toxicity of organic UV filters (ensulizole and octocrylene) in a sentinel marine bivalve Mytilus edulis.

Sci Total Environ 2021 Jul 26;798:149171. Epub 2021 Jul 26.

Department of Marine Biology, Institute for Biological Sciences, University of Rostock, Rostock, Germany; Department of Maritime Systems, Interdisciplinary Faculty, University of Rostock, Rostock, Germany. Electronic address:

The global occurrence of organic UV filters in the marine environment is of increasing ecotoxicological concern. Here we assessed the toxicity of UV filters ensulizole and octocrylene in the blue mussels Mytilus edulis exposed to 10 or 100 μg l of octocrylene and ensulizole for two weeks. An integrated battery of biochemical and molecular biomarkers related to xenobiotics metabolism and cellular toxicity (including oxidative stress, DNA damage, apoptosis, autophagy and inflammation) was used to assess the toxicity of these UV filters in the mussels. Read More

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Towards a more efficient Hydrothermal Carbonization: Processing water recirculation under different conditions.

Waste Manag 2021 Jul 27;132:115-123. Epub 2021 Jul 27.

Laboratory of Process Engineering and Industrial Systems (LR11ES54), National School of Engineering of Gabes, University of Gabes, Medenine Street 6029, Tunisia.

The use of Processing Water (PW) on the Hydrothermal Carbonization (HTC) of tunisian pine cones was investigated under different temperature (200 and 240 °C) and residence time conditions (3 and 20 h). Recirculating PW improved the HC (hydrochar) solid yield, without being detrimental to the carbon content of the HC. The heating value of the HCs, that with a single reaction already involved a rise of this parameter in the range 22. Read More

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CD30L classical monocytes play a pro-inflammatory role in the development of ulcerative colitis in patients.

Mol Immunol 2021 Jul 27;138:10-19. Epub 2021 Jul 27.

Department of Immunology, China Medical University, 110122, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, PR China. Electronic address:

Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disease that affects the large intestine and exhibits a relapsing and remitting course. It is a complex immune-mediated disease of the gastrointestinal tract that increases morbidity and negatively influences the quality of life. Although our previous studies have indicated that CD30L is involved in the adaptive immune response in UC, it remains unclear whether it participates in the innate immune response. Read More

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Overview of oral cavity squamous cell carcinoma: Risk factors, mechanisms, and diagnostics.

Oral Oncol 2021 Jul 27;121:105451. Epub 2021 Jul 27.

Department of Biotechnology, National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), An Institute of National Importance, Government of India, Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Palaj, Opp. Air Force Station, Gandhinagar 382355, Gujarat, India. Electronic address:

Oral cavity squamous cell carcinoma (OCSCC) is the most common malignancy of the oral cavity. The substantial risk factors for OCSCC are the consumption of tobacco products, alcohol, betel quid, areca nut, and genetic alteration. However, technological advancements have occurred in treatment, but the survival decreases with late diagnosis; therefore, new methods are continuously being investigated for treatment. Read More

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Targeting COVID-19 (SARS-CoV-2) main protease through active phytocompounds of ayurvedic medicinal plants - Emblica officinalis (Amla), Phyllanthus niruri Linn. (Bhumi Amla) and Tinospora cordifolia (Giloy) - A molecular docking and simulation study.

Comput Biol Med 2021 Jul 24;136:104683. Epub 2021 Jul 24.

Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, 570 020, Karnataka, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002, Uttar Pradesh, India. Electronic address:

Coronavirus Disease-2019 (COVID-19), a viral disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was declared a global pandemic by WHO in 2020. In this scenario, SARS-CoV-2 main protease (COVID-19 M), an enzyme mainly involved in viral replication and transcription is identified as a crucial target for drug discovery. Traditionally used medicinal plants contain a large amount of bioactives and pave a new path to develop drugs and medications for COVID-19. Read More

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DNA end resection during homologous recombination.

Curr Opin Genet Dev 2021 Jul 27;71:99-105. Epub 2021 Jul 27.

Department of Microbiology & Immunology, Columbia University Irving Medical Center, New York, NY 10032, USA; Department of Genetics & Development, Columbia University Irving Medical Center, New York, NY 10032, USA. Electronic address:

Exposure to environmental mutagens but also cell-endogenous processes can create DNA double-strand breaks (DSBs) in a cell's genome. DSBs need to be repaired accurately and timely to ensure genomic integrity and cell survival. One major DSB repair mechanism, called homologous recombination, relies on the nucleolytic degradation of the 5'-terminated strands in a process termed end resection. Read More

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Integrating RNA-seq with functional expression to analyze the regulation and characterization of genes involved in monoterpenoid biosynthesis in Nepeta tenuifolia Briq.

Plant Physiol Biochem 2021 Jul 23;167:31-41. Epub 2021 Jul 23.

College of Pharmacy, Nanjing University of Chinese Medicine, Medicine, Nanjing, 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210023, China. Electronic address:

Nepeta tenuifolia Briq. (Lamiaceae) is a medicinal plant historically used in the East Asia region to treat cold and fever, and it is currently used as a clinically effective treatment for respiratory diseases. We previously found that monoterpenoids are the dominant volatile secondary metabolites in N. Read More

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Experienced critical incidents affecting lead-times in the stroke care chain for low-priority patients - A qualitative study with critical incident technique.

Int Emerg Nurs 2021 Jul 27;58:101040. Epub 2021 Jul 27.

Institute of Health and Care Sciences, Sahlgrenska Academy, University of Gothenburg, Sweden; Sahlgrenska Universitetssjukhuset, Gothenburg, Sweden; Chalmers University of Technology, Gothenburg, Sweden.

Background: The time elapsing from the alarm being raised to arrival at the stroke unit is crucial for patients suffering a conceivable stroke. More knowledge is needed about critical incidents-both favourable and unfavourable-affecting the lead time in the care chain.

Aim: To explore favourable and unfavourable critical incidents (CIs), affecting lead times in the care chain from the alerted ambulance to the stroke unit, as experienced by nurses, for low-priority patients suffering a conceivable acute stroke. Read More

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Pulmonary rehabilitation outcomes in individuals with chronic obstructive pulmonary disease: a systematic review.

Ann Phys Rehabil Med 2021 Jul 27:101564. Epub 2021 Jul 27.

Lab3R - Respiratory Research and Rehabilitation Laboratory, School of Health Sciences (ESSUA), University of Aveiro, Aveiro, Portugal; iBiMED: Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal. Electronic address:

Background: The magnitude of response to pulmonary rehabilitation (PR) is influenced by the selection of outcomes and measures.

Objectives: This systematic review aimed to review all outcomes and measures used in clinical trials of PR for individuals with chronic obstructive pulmonary disease (COPD).

Methods: The review involved a search of Scopus, Web of Knowledge, Cochrane Library, EBSCO, Science Direct and PubMed databases for studies of stable individuals with COPD undergoing PR. Read More

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Adult-born proopiomelanocortin neurons derived from Rax-expressing precursors mitigate the metabolic effects of congenital hypothalamic proopiomelanocortin deficiency.

Mol Metab 2021 Jul 27:101312. Epub 2021 Jul 27.

Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, Tufts Medical Center, 800 Washington St, Boston, MA, 02111, USA.

Objective: Proopiomelanocortin (POMC) neurons of the hypothalamic arcuate nucleus are essential regulators of energy balance. Selective loss of POMC production in these cells results in extreme obesity and metabolic comorbidities. While neurogenesis occurs in the adult hypothalamus, it remains uncertain whether functional POMC neurons emerge during adulthood in physiologically significant numbers. Read More

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Computational model of trachea - alveoli gas movement during spontaneous breathing.

Respir Physiol Neurobiol 2021 Jul 27:103767. Epub 2021 Jul 27.

Universidad EIA, Envigado, Antioquia, Colombia. Electronic address:

A computational model of the transport of gases involved in spontaneous breathing, from the trachea inlet to the alveoli was developed for healthy patients. Convective and diffusive transport mechanisms were considered simultaneously, using a diffusion coefficient (D) that has considered the four main species of gases present in the exchange carried out by the human lung, nitrogen (N), oxygen (O), carbon dioxide (CO) and water vapor (HO). A Matlab® script was programmed to simulate the trachea-alveolus gas exchange model under three respiratory frequencies: 12, 24 and 40 breaths per minute (BPM), each with three diaphragmatic movements of 2 cm, 4 cm, and 6 cm. Read More

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The lamprey respiratory network: some evolutionary aspects.

Respir Physiol Neurobiol 2021 Jul 27:103766. Epub 2021 Jul 27.

Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, Università degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy. Electronic address:

Breathing is a complex behaviour that involves rhythm generating networks. In this review, we examine the main characteristics of respiratory rhythm generation in vertebrates and, in particular, we describe the main results of our studies on the role of neural mechanisms involved in the neuromodulation of the lamprey respiration. The lamprey respiratory rhythm generator is located in the paratrigeminal respiratory group (pTRG) and shows similarities with the mammalian preBötzinger complex. Read More

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PCNA inhibition enhances the cytotoxicity of β-lapachone in NQO1-Positive cancer cells by augmentation of oxidative stress-induced DNA damage.

Cancer Lett 2021 Jul 27. Epub 2021 Jul 27.

Department of Radiation Oncology, Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. Electronic address:

β-Lapachone is a classic quinone-containing antitumor NQO1-bioactivatable drug that directly kills NQO1-overexpressing cancer cells. However, the clinical applications of β-lapachone are primarily limited by its high toxicity and modest lethality. To overcome this side effect and expand the therapeutic utility of β-lapachone, we demonstrate the effects of a novel combination therapy including β-lapachone and the proliferating cell nuclear antigen (PCNA) inhibitor T2 amino alcohol (T2AA) on various NQO1 cancer cells. Read More

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Study on the alleviation of Fengshi Gutong capsule on rheumatoid arthritis through integrating network pharmacology and experimental exploration.

J Ethnopharmacol 2021 Jul 27:114471. Epub 2021 Jul 27.

The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Ethnopharmacological Relevance: Fengshi Gutong (FSGT) capsule, a traditional Chinese medicine formula, has effects including warming meridians and dispersing cold, and relieving pain by dredging collaterals. FSGT is generally used for the treatment of rheumatoid arthritis (RA) in clinic in China.

Aim Of The Study: This study aims to investigate the alleviation provided by FSGT capsule on RA in vivo and the engaged mechanism. Read More

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Analgesic effect of the main components of Corydalis yanhusuo (yanhusuo in Chinese) is caused by inhibition of voltage gated sodium channels.

J Ethnopharmacol 2021 Jul 27:114457. Epub 2021 Jul 27.

School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, Shenyang, Liaoning, 110016, China. Electronic address:

Ethnopharmacology Relevance: Pain often causes a series of abnormal changes in physiology and psychology, which can lead to disease and even death. Drug therapy is the most basic and commonly used method for pain relief and management. Interestingly, at present, hundreds of traditional Chinese medicines have been reported to be used for pain relief, most of which are monomer preparations, which have been developed into new painkillers. Read More

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Effects of dopamine transporter changes in the ventral tegmental area of the midbrain on cognitive function in aged rats.

J Chem Neuroanat 2021 Jul 27:102009. Epub 2021 Jul 27.

Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, 832000, China. Electronic address:

The pathogenesis of Perioperative neurocognitive disorders (PND) is a synergistic effect of many factors. Up to now, the exact mechanism remains unclear. The dopamine pathway in the brain is one of the paths involved in the means of cognitive function. Read More

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Biochemical, crystallographic and biophysical characterization of histidine triad nucleotide-binding protein 2 with different ligands including a non-hydrolyzable analog of Ap4A.

Biochim Biophys Acta Gen Subj 2021 Jul 27:129968. Epub 2021 Jul 27.

Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.

Background: Human HINT2 is an important mitochondrial enzyme involved in many processes such as apoptosis and bioenergetics, but its endogenous substrates and the three-dimensional structure of the full-length protein have not been identified yet.

Methods: An HPLC assay was used to test the hydrolytic activity of HINT2 against various adenosine, guanosine, and 2'-deoxyguanosine derivatives containing phosphate bonds of different types and different leaving groups. Data on binding affinity were obtained by microscale thermophoresis (MST). Read More

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Biased and allosteric modulation of bone cell-expressing G protein-coupled receptors as a novel approach to osteoporosis therapy.

Pharmacol Res 2021 Jul 27:105794. Epub 2021 Jul 27.

Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, 6905126, Israel; Adelson School of Medicine, Ariel University, Ariel, 4077625, Israel. Electronic address:

On the cellular level, osteoporosis (OP) is a result of imbalanced bone remodeling, in which osteoclastic bone resorption outcompetes osteoblastic bone formation. Currently available OP medications include both antiresorptive and bone-forming drugs. However, their long-term use in OP patients, mainly in postmenopausal women, is accompanied by severe side effects. Read More

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Competitive binding of extracellular accumulated heparan sulfate reduces lysosomal storage defects and triggers neuronal differentiation in a model of Mucopolysaccharidosis IIIB.

Biochim Biophys Acta Mol Cell Res 2021 Jul 27:119113. Epub 2021 Jul 27.

Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Sergio Pansini 5, 80131, Naples, Italy. Electronic address:

Mucopolysaccharidoses (MPSs) are a group of inherited lysosomal storage disorders associated with the deficiency of lysosomal enzymes involved in glycosaminoglycan (GAG) degradation. The resulting cellular accumulation of GAGs is responsible for widespread tissue and organ dysfunctions. The MPS III, caused by mutations in the genes responsible for the degradation of heparan sulfate (HS), includes four subtypes (A, B, C, and D) that present significant neurological manifestations such as progressive cognitive decline and behavioral disorders. Read More

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Thrombospondin 1 and 2 along with PEDF inhibit angiogenesis and promote lymphangiogenesis in intrahepatic cholangiocarcinoma.

J Hepatol 2021 Jul 27. Epub 2021 Jul 27.

Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy. Electronic address:

Background & Aims: Intrahepatic cholangiocarcinoma (iCCA) microenvironment is hypovascularized, with a prominent development of lymphatic vessels. This leads to a rapid cancer spread into regional lymph nodes and the liver parenchyma, precluding curative treatments. Here, we investigated which factors released in the iCCA stroma drive the inhibition of angiogenesis, promoting lymphangiogenesis. Read More

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Experimental assessment of inter-centre variation in stopping-power and range prediction in particle therapy.

Radiother Oncol 2021 Jul 27. Epub 2021 Jul 27.

OncoRay - National Center for Radiation Research in Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Helmholtz-Zentrum Dresden - Rossendorf, Dresden, Germany; Helmholtz-Zentrum Dresden - Rossendorf, Institute of Radiooncology - OncoRay, Dresden, Germany; Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; German Cancer Consortium (DKTK), partner site Dresden, and German Cancer Research Center (DKFZ), Heidelberg, Germany.

Purpose: Experimental assessment of inter-centre variation and absolute accuracy of stopping-power-ratio (SPR) prediction within 17 particle therapy centres of the European Particle Therapy Network.

Material And Methods: A head and body phantom with seventeen tissue-equivalent materials were scanned consecutively at the participating centres using their individual clinical CT scan protocol and translated into SPR with their in-house CT-number-to-SPR conversion. Inter-centre variation and absolute accuracy in SPR prediction were quantified for three tissue groups: lung, soft tissues and bones. Read More

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Interferon-beta, interferon-gamma and their fusion interferon of Siberian tigers (Panthera tigris altaica) in China are involved in positive-feedback regulation of interferon production.

Dev Comp Immunol 2021 Jul 27:104211. Epub 2021 Jul 27.

College of wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China. Electronic address:

As a group of cytokines, interferons are the first line of defense in the antiviral immunity. In this study, Siberian tiger IFN-β (PtIFN-β) and IFN-γ (PtIFN-γ) were successfully amplified, and the two were fused (PtIFN-γ) by overlap extension polymerase chain reaction (SOE-PCR). Bioinformatics analysis disclosed that PtIFN-β and PtIFN-γ have species-specificity and conservation in the course of evolution. Read More

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