11,865 results match your criteria investigation profiles

Multimodal investigation of rat hepatitis E virus antigenicity: implications for infection, diagnostics, and vaccine efficacy.

J Hepatol 2021 Jan 15. Epub 2021 Jan 15.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong; Carol Yu Centre for Infection, The University of Hong Kong; The Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong. Electronic address:

Background & Aims: Rat hepatitis E virus (Orthohepevirus species C; HEV-C1) is an emerging cause of viral hepatitis in humans. HEV-C1 is divergent from other HEV variants infecting humans that belong to Orthohepevirus species A (HEV-A). This study assessed HEV-C1 antigenic divergence from HEV-A and investigated the impact of this divergence on infection susceptibility, serological test sensitivity, and vaccine efficacy. Read More

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January 2021

Temozolomide with irinotecan versus temozolomide, irinotecan plus bevacizumab for recurrent medulloblastoma of childhood: Report of a COG randomized Phase II screening trial.

Pediatr Blood Cancer 2021 Apr 12:e29031. Epub 2021 Apr 12.

Saint Jude Children's Research Hospital, Memphis, Tennessee, USA.

Background: Approximately 30% of children with medulloblastoma (MB) experience recurrence, which is usually incurable. This study compared the overall survival (OS) of patients receiving temozolomide (TMZ) and irinotecan with that of patients receiving TMZ, irinotecan, and bevacizumab for recurrent MB/central nervous system (CNS) primitive neuroectodermal tumor (PNET).

Methods: Patients with relapsed/refractory MB or CNS PNET were randomly assigned to receive TMZ (150 mg/m /day PO on days 1-5) and irinotecan (50 mg/m /day IV on days 1-5) with or without bevacizumab (10 mg/kg IV on days 1 and 15). Read More

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Temperature but not ocean acidification affects energy metabolism and enzyme activities in the blue mussel, .

Ecol Evol 2021 Apr 5;11(7):3366-3379. Epub 2021 Mar 5.

Department of Biological Sciences University of North Carolina at Charlotte Charlotte NC USA.

In mosaic marine habitats, such as intertidal zones, ocean acidification (OA) is exacerbated by high variability of pH, temperature, and biological CO production. The nonlinear interactions among these drivers can be context-specific and their effect on organisms in these habitats remains largely unknown, warranting further investigation.We were particularly interested in (the blue mussel) from intertidal zones of the Gulf of Maine (GOM), USA, for this study. Read More

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Gut microbiota profiles and characterization of cultivable fungal isolates in IBS patients.

Appl Microbiol Biotechnol 2021 Apr 10. Epub 2021 Apr 10.

Department of Agriculture and Food Sciences, University of Bologna, Viale Fanin 44, 40127, Bologna, Italy.

Studies so far conducted on irritable bowel syndrome (IBS) have been focused mainly on the role of gut bacterial dysbiosis in modulating the intestinal permeability, inflammation, and motility, with consequences on the quality of life. Limited evidences showed a potential involvement of gut fungal communities. Here, the gut bacterial and fungal microbiota of a cohort of IBS patients have been characterized and compared with that of healthy subjects (HS). Read More

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Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation.

Aging (Albany NY) 2021 Apr 4;13. Epub 2021 Apr 4.

The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.

Autologous chondrocyte implantation (ACI) is an effective method for treating chronic articular cartilage injury and degeneration; however, it requires large numbers of hyaline chondrocytes, and human hyaline chondrocytes often undergo dedifferentiation . Moreover, although long non-coding RNAs (lncRNAs) regulate gene expression in many pathological and physiological processes, their role in human hyaline chondrocyte dedifferentiation remains unclear. Here, we examined lncRNA and mRNA expression profiles in human hyaline chondrocyte dedifferentiation using microarray analysis. Read More

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Extensive phytocannabinoid profiles of seized cannabis and cannabis-based medicines - Identification of potential distinguishing markers.

Forensic Sci Int 2021 Mar 31;322:110773. Epub 2021 Mar 31.

Institute of Legal Medicine, University Medical Center of the Johannes Gutenberg University Mainz, Am Pulverturm 3, 55131 Mainz, Germany.

As the frequency of cannabis-based therapy increases, the ability to distinguish intake of cannabis-based medicines from recreational cannabis use becomes desirable. Minor cannabinoids have been suggested to indicate recreational cannabis use in biological matrices but are unreliable when presumably also present in directly plantderived medicines. Thus, for therapeutics such as medical cannabis, Sativex® and Dronabinol, a more thorough investigation of cannabinoid profiles is required to identify possible distinguishing markers. Read More

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Genetic and serological characterization of capsular antigen untypeable Vibrio parahaemolyticus strains reveal novel K serotypes and epidemiological characteristics in Shandong, China.

Int J Food Microbiol 2021 Mar 31;347:109188. Epub 2021 Mar 31.

TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin 300457, PR China. Electronic address:

Vibrio parahaemolyticus, which is commonly found in marine and estuarine environments worldwide and isolated from aquatic products, is one of the most important food-borne pathogens. Among the various typing methods, serotyping is widely accepted and utilized by infectious disease specialists and infection control agencies for the detection and epidemiological investigation of this pathogen. Thus far, 13 O serotypes and 71 K serotypes have been defined; however, untypeable strains are frequently isolated during routine detection, and some new O and/or K antigens have been identified and characterized. Read More

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Determination of antimicrobial resistance profiles and mechanisms of using a Raman spectroscopy-based metabolomic approach.

Appl Environ Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

Rapid identification of antimicrobial resistance (AMR) profiles and mechanisms is critical for clinical management and drug development. However, the current AMR detection approaches take up to 48 h to obtain a result. Here we demonstrate a Raman spectroscopy-based metabolomic approach to rapidly determine the AMR profile of , a major cause of foodborne gastroenteritis worldwide. Read More

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Transcriptomic investigation of embryonic pectoral muscle reveals increased myogenic processes in Shitou geese compared to Wuzong geese.

Br Poult Sci 2021 Apr 9. Epub 2021 Apr 9.

College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Embryonic stages before birth are crucial for poultry muscle development, as this determines muscle mass in adulthood. This study characterised the distinction in embryonic pectoral muscle development between Wuzong (WZE, small) and Shitou (STE, large) geese (two indigenous goose breeds in Guangdong Province, China) at embryonic days 15 (E15), 23 (E23) and the day of hatching (P1) to gain insights into the regulatory mechanisms of muscle development.The results showed that STE had significantly higher myofibre density during E15-P1 and had significantly larger myofibre diameter at E15 than WZE. Read More

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A hybrid multi-objective whale optimization algorithm for analyzing microarray data based on Apache Spark.

PeerJ Comput Sci 2021 25;7:e416. Epub 2021 Mar 25.

Faculty of Computers and Information, Assiut University, Egypt.

A microarray is a revolutionary tool that generates vast volumes of data that describe the expression profiles of genes under investigation that can be qualified as Big Data. Hadoop and Spark are efficient frameworks, developed to store and analyze Big Data. Analyzing microarray data helps researchers to identify correlated genes. Read More

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LSD1 as a Biomarker and the Outcome of Its Inhibitors in the Clinical Trial: The Therapy Opportunity in Tumor.

J Oncol 2021 25;2021:5512524. Epub 2021 Mar 25.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

Tumors are the foremost cause of death worldwide. As a result of that, there has been a significant enhancement in the investigation, treatment methods, and good maintenance practices on cancer. However, the sensitivity and specificity of a lot of tumor biomarkers are not adequate. Read More

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Characterization of simultaneous uptake of xylose and glucose in Caldicellulosiruptor kronotskyensis for optimal hydrogen production.

Biotechnol Biofuels 2021 Apr 8;14(1):91. Epub 2021 Apr 8.

Division of Applied Microbiology, Lund University, P.O. Box 124, 221 00, Lund, Sweden.

Background: Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was to investigate the growth profiles of C. kronotskyensis in the presence of mixtures of glucose-xylose. Read More

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Altered subcutaneous adipose tissue parameters after switching ART-controlled HIV+ patients to raltegravir/maraviroc.

AIDS 2021 Apr 6. Epub 2021 Apr 6.

Sorbonne Université, Inserm, Faculty of Medicine, Centre de Recherche Saint-Antoine (CRSA), ICAN, Paris, France Department of Biochemistry-Pharmacology-Molecular Biology, APHP, Henri-Mondor Hospital, Université Paris Est Créteil, France Sorbonne Université, Inserm, Faculty of Medicine, Nutrition and obesities: systemic approaches (NutriOmics), Paris, France Assistance Publique Hôpitaux de Paris, Sorbonne Université, Nutrition department, Pitié-Salpétrière hospital, CRNH Ile-de-France, GH APHP-Sorbonne Université, Paris, France Department of Endocrinology, CRMR Prisis, Saint-Antoine Hospital, GH APHP-Sorbonne Université, Paris France Sorbonne Université, Inserm, Faculty of Medicine, Institut Pierre Louis d'Epidémiologie et de Santé Publique (IPLESP), Paris, France IRD, Sorbonne University, UMMISCO, Bondy, France Department of Infectious Diseases, Pitié-Salpétrière hospital, GH APHP-Sorbonne Université, Paris France.

Objective: To evaluate the effect on anthropometric, metabolic and adipose tissue parameters of switching ART-controlled persons living with HIV (PLWH) from a protease-inhibitor regimen to raltegravir/maraviroc.

Design: Substudy of ROCnRAL-ANRS157 with investigation of subcutaneous abdominal adipose tissue (SCAT) biopsy at inclusion and study end.

Methods: We performed lipoaspiration of paired SCAT samples, histology on fresh/fixed samples and examined the transcriptomic profile analyzed using Illumina microarrays after RNA extraction. Read More

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Toward Exotic Silicon Doping with a Low Thermal Budget and Flexible Profile Control by Liquid-Phase Epitaxy.

ACS Appl Mater Interfaces 2021 Apr 8. Epub 2021 Apr 8.

Department of Nanoengineering, University of California San Diego, La Jolla, California 92093, United States.

We investigate semiconductor p-n junction formation by liquid-phase epitaxy (LPE) using metallic pastes incorporating traditional and nontraditional dopants. The LPE technique enables us to control the shape of doping profiles with a low thermal budget through the choice of solvent, total amount of solvent deposited, and process temperature. We focus here on the Al-B, Zn-P, and Sn-Ga chemistries to dope silicon regions using the chemicophysical properties of a low-eutectic-temperature metallic solvent acting as a matrix for the dissolution of a high concentration of a dopant. Read More

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Pineapple consumption reduced cardiac oxidative stress and inflammation in high cholesterol diet-fed rats.

Nutr Metab (Lond) 2021 Apr 7;18(1):36. Epub 2021 Apr 7.

Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.

Background: Hypercholesterolemia is a major risk factor for cardiovascular disease. It has been reported that pineapple contains healthy nutrients and phytochemicals associated with antioxidant and anti-inflammatory capacities. No investigation exists concerning the effect of pineapple consumption modulating hypercholesterolemia-induced cardiac damage in high-cholesterol diet (HCD)-fed rats. Read More

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Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures.

PLoS Negl Trop Dis 2021 Apr 7;15(4):e0009320. Epub 2021 Apr 7.

Infectious Bacterial Disease Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, Iowa, United States of America.

Background: Leptospirosis is a zoonotic, bacterial disease, posing significant health risks to humans, livestock, and companion animals around the world. Symptoms range from asymptomatic to multi-organ failure in severe cases. Complex species-specific interactions exist between animal hosts and the infecting species, serovar, and strain of pathogen. Read More

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Variations in IgG antibody subclass responses to oral bacteria: Effects of periodontal disease and modifying factors.

J Periodontal Res 2021 Apr 7. Epub 2021 Apr 7.

Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA.

Background And Objective: Local and systemic IgG antibodies or oral bacteria have been described with periodontitis. We extended these observations by assessing the impact of a range of intrinsic factors on serum IgG subclass antibodies to both commensal and pathogenic oral bacteria that would contribute to variations in immune protection or disease susceptibility in periodontitis have not been described.

Methods: Subjects (n = 278) were classified as healthy, gingivitis, or periodontitis and categorized as mild, moderate, and severe periodontitis. Read More

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J Wildl Dis 2021 Apr;57(2):345-356

Graduate Institute of Veterinary Clinical Science, National Taiwan University, 153, section 3, Keelung Rd., Taipei, 106, Taiwan.

Parasite infection is one of the most important factors in wildlife conservation. However, fecal parasite profiles of threatened Asiatic black bears (Ursus thibetanus) are only sporadically reported, and the effect of parasitic diseases on the survival of the locally endangered Formosan black bear (Ursus thibetanus formosanus) in Taiwan remains undetermined. The study objective was to investigate the gastrointestinal parasite profiles of Formosan black bears in Yushan National Park, the only known high-density habitat for the species in Taiwan. Read More

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Latent Class Analysis of Individual-Level Characteristics Predictive of Intervention Outcomes in Urban Male Adolescents.

Res Child Adolesc Psychopathol 2021 Apr 5. Epub 2021 Apr 5.

RTI International, Research Triangle Park, Durham, North Carolina, USA.

Preventive intervention research dictates that new techniques are needed to elucidate what types of interventions work best for whom to prevent behavioral problems. The current investigation applies a latent class modeling structure to identify the constellation of characteristics-or profile-in urban male adolescents (n = 125, aged 15) that interrelatedly predict responses to a brief administration of an evidence-based program, Positive Adolescent Choices Training (PACT). Individual-level characteristics were selected as predictors on the basis of their association with risk behaviors and their implication in intervention outcomes (e. Read More

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Emotions of COVID-19: A Study of Self-Reported Information and Emotions during the COVID-19 Pandemic using Artificial Intelligence.

J Med Internet Res 2021 Apr 1. Epub 2021 Apr 1.

Research Centre for Data Analytics and Cognition, La Trobe University, Kingsbury Drive, Bundoora, AU.

Background: The COVID-19 pandemic has disrupted human societies across the world. Starting with a public health emergency, followed by a significant loss of human life, and the ensuing social restrictions leading to loss of employment, lack of interactions and burgeoning psychological distress. As physical distancing regulations were introduced to manage outbreaks, individuals, groups and communities engaged extensively on social media to express their thoughts and emotions. Read More

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Systematic Characterization of Expression Profiles and Prognostic Values of the Eight Subunits of the Chaperonin TRiC in Breast Cancer.

Front Genet 2021 17;12:637887. Epub 2021 Mar 17.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: Chaperonin-containing TCP-1 (TRiC or CCT) was demonstrated to be involved in oncogenesis of cancers carcinogenesis and development of various malignancies. Increasing experimental evidence indicated that dysregulation of TRiC was implicated in the tumor progression of breast cancer (BCa). However, few definitive studies have addressed the diverse expression patterns and prognostic values of eight TRiC subunits. Read More

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Immune Cell Infiltration-Based Characterization of Triple-Negative Breast Cancer Predicts Prognosis and Chemotherapy Response Markers.

Front Genet 2021 19;12:616469. Epub 2021 Mar 19.

Department of Anatomy, Harbin Medical University, Harbin, China.

Breast cancer represents the number one cause of cancer-associated mortality globally. The most aggressive molecular subtype is triple negative breast cancer (TNBC), of which limited therapeutic options are available. It is well known that breast cancer prognosis and tumor sensitivity toward immunotherapy are dictated by the tumor microenvironment. Read More

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Effects of metabolizable protein concentration, amino acid profile, and fiber source on the messenger RNA expression of skeletal muscle in peripartum dairy cows.

J Dairy Sci 2021 Apr 1. Epub 2021 Apr 1.

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691. Electronic address:

After parturition, dairy cows mobilize AA from skeletal muscle to meet metabolizable protein (MP) requirements. High mobilization may compromise cow health and longer-term milk production. Postpartum diets with higher MP concentrations, improved AA profiles, or MP increased at the expense of forages rather than nonforage fiber sources may attenuate muscle catabolism; however, the molecular mechanisms responsible need investigation. Read More

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New Polyketides from the Antarctic Fungus sp. HSX2#-11.

Mar Drugs 2021 Mar 22;19(3). Epub 2021 Mar 22.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266200, China.

The species is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus sp. Read More

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Triterpenes and Phenolic Compounds from the Fungus : Isolation, Structure Determination and Biological Activity.

Molecules 2021 Mar 16;26(6). Epub 2021 Mar 16.

Department of Pharmacognosy, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary.

Investigation of the methanol extract of the poroid fungus resulted in the isolation of a novel triterpene, fuscoporic acid (), together with inoscavin A and its previously undescribed isomer ( and ), 3,4-dihydroxy-benzaldehide (), osmundacetone (), senexdiolic acid (), natalic acid (), and ergosta-7,22-diene-3-one (). The structures of fungal compounds were determined on the basis of NMR and MS spectroscopic analyses, as well as molecular modeling studies. Compounds , - were examined for their antibacterial properties on resistant clinical isolates, and cytotoxic activity on human colon adenocarcinoma cell lines. Read More

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Large Scale Molecular Studies of Pituitary Neuroendocrine Tumors: Novel Markers, Mechanisms and Translational Perspectives.

Cancers (Basel) 2021 Mar 19;13(6). Epub 2021 Mar 19.

Latvian Biomedical Research and Study Centre, Ratsupites Str. 1-k1, LV-1067 Riga, Latvia.

Pituitary neuroendocrine tumors (PitNETs) are non-metastatic neoplasms of the pituitary, which overproduce hormones leading to systemic disorders, or tumor mass effects causing headaches, vertigo or visual impairment. Recently, PitNETs have been investigated in large scale (exome and genome) molecular analyses (transcriptome microarrays and sequencing), to uncover novel markers. We performed a literature analysis on these studies to summarize the research data and extrapolate overlapping gene candidates, biomarkers, and molecular mechanisms. Read More

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Microbiologic Profiles of Patients with Dental Prosthetic Treatment and Periodontitis before and after Photoactivation Therapy-Randomized Clinical Trial.

Microorganisms 2021 Mar 30;9(4). Epub 2021 Mar 30.

Department of Dental Technology, "Grigore T. Popa" University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania.

Fixed prosthodontic dental restorations can potentially affect the periodontal tissues and vice versa, the periodontium can influence the longevity and esthetic appearance of dental restorations. We proposed an investigation on total bacterial load, specific periodontal pathogens, and periodontal clinical parameters in patients with dental fixed prosthesis and different degrees of periodontal tissue loss that followed photoactivation therapy (PDT) adjunctive to scaling and root planing. The study was conducted on 160 subjects, which were randomly assigned to scaling and root planing (SRP) alone (52 subjects, 256 sites), SRP and chlorhexidine rinsing (58 subjects, 276 sites), and SRP plus PDT (50 subjects, 318 sites). Read More

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Hesperidin and Naringin Improve Broiler Meat Fatty Acid Profile and Modulate the Expression of Genes Involved in Fatty Acid β-oxidation and Antioxidant Defense in a Dose Dependent Manner.

Foods 2021 Mar 31;10(4). Epub 2021 Mar 31.

Laboratory of Animal Breeding and Husbandry, Department of Animal Science, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.

The beneficial properties of the flavanones hesperidin and naringin as feed additives in poultry have lately been under investigation. In broilers, both flavanones have been shown to exhibit antioxidant properties while their individual effects on fatty acid (FA) composition and the underlying molecular mechanisms of their activity have not been explored. Here, we studied their effects on broiler meats' FA profiles and on the expression of genes related to lipid metabolism, antioxidant defense and anti-inflammatory function. Read More

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Shelf-Life Evolution of the Fatty Acid Fingerprint in High-Quality Hazelnuts ( L.) Harvested in Different Geographical Regions.

Foods 2021 Mar 23;10(3). Epub 2021 Mar 23.

Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, I-10125 Torino, Italy.

Hazelnuts are characterized by a relatively high abundance of oleic acid and poly-unsaturated fatty acids, which give this fruit a high nutritional value. As a counterbalance, such a lipid profile is more susceptible to autoxidation and/or degradation reactions under enzymatic catalysis. Lipid oxidation occurs on fatty acids (FAs), both esterified on triacylglycerols and in free form (after lipolysis), but with favorable kinetics on the latter. Read More

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D-α-Tocopherol-Based Micelles for Successful Encapsulation of Retinoic Acid.

Pharmaceuticals (Basel) 2021 Mar 4;14(3). Epub 2021 Mar 4.

Department of Pharmacy (DiFAR), University of Genoa, Viale Cembrano 4, 16148 Genova, Italy.

All--retinoic acid (ATRA) represents the first-choice treatment for several skin diseases, including epithelial skin cancer and acne. However, ATRA's cutaneous side effects, like redness and peeling, and its high instability limit its efficacy. To address these drawbacks and to improve ATRA solubilization, we prepared ATRA-loaded micelles (ATRA-TPGSs), by its encapsulation in D-α-tocopheryl-polyethylene-glycol-succinate (TPGS). Read More

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