48,819 results match your criteria investigated microbial


Adsorption and leaching of β-blockers in fluvo-aquic and black soil: Behavior characteristic and enantiomer selectivity.

Environ Res 2022 Aug 9:114062. Epub 2022 Aug 9.

MOE Key Laboratory of Regional Environment and Eco-restoration, College of Environment, Shenyang University, Shenyang, 110044, China.

β-blockers are widely used chiral pharmaceuticals to treat hypertension and cardiovascular diseases, which are ubiquitously detected in the water-soil environment. However, little is known about their biogeochemical behaviors and enantiomer selectivity during soil migration and transformation. In this study, the adsorption and leaching behaviors of β-blockers in fluvo-aquic soil and black soil were investigated. Read More

View Article and Full-Text PDF

Distinct effects of fiber and colon segment on microbiota-derived indoles and short-chain fatty acids.

Food Chem 2022 Jul 27;398:133801. Epub 2022 Jul 27.

Host-Microbe Interactomics Group, Department of Animal Sciences, Wageningen University, P.O. Box 17, 6700 AA Wageningen, the Netherlands. Electronic address:

Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem. Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment. Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin. Read More

View Article and Full-Text PDF

Drug susceptibility profiling and genetic determinants of drug resistance in Mycobacterium simiae isolates obtained from regional tuberculosis reference laboratories of Iran.

PLoS One 2022 12;17(8):e0267320. Epub 2022 Aug 12.

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background: Among Non-tuberculous mycobacteria (NTM) which generally cause opportunistic infections, especially in immunocompromised hosts, Mycobacterium simiae (M. simiae) is one of the most important NTM, associated with pulmonary disease. The main concern about M. Read More

View Article and Full-Text PDF

The microbial communities in , free amino acids in raw liquor, and their correlations for raw liquor production.

Food Sci Nutr 2022 Aug 8;10(8):2681-2693. Epub 2022 Apr 8.

College of Wuliangye Technology and Food Engineering & College of Modern Agriculture Yibin Vocational and Technical College Yibin China.

liquor (WLFL), a specific Chinese traditional liquor, one of the major type of global distilled spirits, offers a unique flavor system acquired across thousands of years of development. Free amino acids (FAAs), as major health factors, are considered to be primarily derive from the hydrolysis of protein from the (ZPs) by microbial populations during fermentation. Here, we investigated the changes of microbial communities in ZPs and FAAs in raw liquor (RL) directly related ZPs from different ages of WLFL fermentation pits by phospholipid fatty acid fingerprint (PLFA) and high performance liquid chromatography method. Read More

View Article and Full-Text PDF

Effects of Xinjiang wild cherry plum ( Ledeb) anthocyanin-rich extract on the plasma metabolome of atherosclerotic apoE-deficient mice fed a high-fat diet.

Front Nutr 2022 25;9:923699. Epub 2022 Jul 25.

Department of Pharmacy, Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

It is well-known that many vegetables and fruits have abundant polyphenols, such as anthocyanins, which benefit many cardiovascular diseases due to their anti-oxidative and anti-inflammatory effects. To explore the protective effect of anthocyanin on atherosclerosis from a metabolic perspective, alterations in plasma metabolic profiling of apoE-deficient (apoE) mice in response to treatment with anthocyanin extracts derived from Xinjiang wild cherry plum ( Ledeb) peel was investigated through UHPLC-Q-TOF/MS. The mice were fed with a normal diet or high-fat diet supplementation with or without anthocyanin extracts (ACNE, 75, 150, 250 mg/kg body weight) for 18 weeks, corresponding to control (Con), model (Mod), and treatment group (LD, low dose; MD, medium dose; HD, high dose), respectively, along with a positive control group (posCon, treatment with Atorvastatin, 0. Read More

View Article and Full-Text PDF

Soil pH Determines the Spatial Distribution, Assembly Processes, and Co-existence Networks of Microeukaryotic Community in Wheat Fields of the North China Plain.

Front Microbiol 2022 25;13:911116. Epub 2022 Jul 25.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.

Soil microeukaryotes play a pivotal role in soil nutrient cycling and crop growth in agroecosystems. However, knowledge of microeukaryotic community distribution patterns, assembly processes, and co-existence networks is greatly limited. Here, microbial eukaryotes in bulk and rhizosphere soils of the North China Plain were investigated. Read More

View Article and Full-Text PDF

Mercury Methylation Potentials in Sediments of an Ancient Cypress Wetland Using Species-Specific Isotope Dilution GC-ICP-MS.

Molecules 2022 Aug 1;27(15). Epub 2022 Aug 1.

Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA.

Wetlands are of a considerable environmental value as they provide food and habitat for plants and animals. Several important chemical transformations take place in wetland media, including the conversion of inorganic mercury (Hg) to monomethylmercury (MeHg), a toxic compound with a strong tendency for bioconcentration. Considering the fact that wetlands are hotspots for Hg methylation, we investigated, for the first time, Hg methylation and demethylation rates in an old growth cypress wetland at Sky Lake in the Mississippi Delta. Read More

View Article and Full-Text PDF

Effects of Different Microbial Fertilizers on Growth and Rhizosphere Soil Properties of Corn in Newly Reclaimed Land.

Plants (Basel) 2022 Jul 29;11(15). Epub 2022 Jul 29.

Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

Land reclamation may expand the supply of usable land for food security. Soil microorganisms have been considered as an amendment in immature soil to improve its quality. However, different microbial fertilizers' effects on plant growth in immature soil have largely been unexplored. Read More

View Article and Full-Text PDF

Therapeutic Role of Curcumin in Diabetes: An Analysis Based on Bioinformatic Findings.

Nutrients 2022 Aug 8;14(15). Epub 2022 Aug 8.

Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: Diabetes is an increasingly prevalent global disease caused by the impairment in insulin production or insulin function. Diabetes in the long term causes both microvascular and macrovascular complications that may result in retinopathy, nephropathy, neuropathy, peripheral arterial disease, atherosclerotic cardiovascular disease, and cerebrovascular disease. Considerable effort has been expended looking at the numerous genes and pathways to explain the mechanisms leading to diabetes-related complications. Read More

View Article and Full-Text PDF

Alterations in Intestinal Brush Border Membrane Functionality and Bacterial Populations Following Intra-Amniotic Administration () of Nicotinamide Riboside and Its Derivatives.

Nutrients 2022 Jul 29;14(15). Epub 2022 Jul 29.

Department of Food Science, Cornell University, Ithaca, NY 14853, USA.

Nicotinamide riboside (NR) acts as a nicotinamide adenine dinucleotide (NAD+) precursor where NR supplementation has previously been shown to be beneficial. Thus, we synthesized and characterized nicotinamide riboside tributyrate chloride (NRTBCl, water-soluble) and nicotinamide riboside trioleate chloride (NRTOCl, oil-soluble) as two new ester derivatives of nicotinamide riboside chloride (NRCl). NRCl and its derivatives were assessed in vivo, via intra-amniotic administration (), with the following treatment groups: (1) non-injected (control); and injection of (2) deionized HO (control); (3) NRCl (30 mg/mL dose); (4) NRTBCl (30 mg/mL dose); and (5) NRTOCl (30 mg/mL dose). Read More

View Article and Full-Text PDF

Enhanced Natural Attenuation of Groundwater Cr(VI) Pollution Using Electron Donors: Yeast Extract vs. Polyhydroxybutyrate.

Int J Environ Res Public Health 2022 Aug 4;19(15). Epub 2022 Aug 4.

Water Research Institute, Italian National Research Council (IRSA-CNR), 70132 Bari, BA, Italy.

Remediation interventions based on the native bacteria's capability to reduce Cr(VI) represent a valid strategy in terms of economic and environmental sustainability. In this study, a bioremediation test was carried out using viable microcosms set with groundwater and deep soil (4:1), collected from the saturated zone of an industrial site in Southern Italy that was polluted by ~130 µg L of Cr(VI). Conditions simulating the potential were compared to the induced by supplying yeast extract or polyhydroxybutyrate. Read More

View Article and Full-Text PDF

The Dual Roles of Nano Zero-Valent Iron and Zinc Oxide in Antibiotics Resistance Genes (ARGs) Spread in Sediment.

Int J Environ Res Public Health 2022 Jul 31;19(15). Epub 2022 Jul 31.

College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China.

Nanoparticles (NPs) are widely used and ubiquitous in the environment, but the consequences of their release into the environment on antibiotics resistance genes (ARGs), microbial abundance, and community, are largely unknown. Therefore, this study examined the effect of nano zero-valent iron (nZVI) and zinc oxide (nZnO) on tetracycline resistance genes (tet-ARGs) and class 1 integron () in sediment under laboratory incubation. The coexistence of NPs and tetracycline (TC) on tet-ARGs/ was also investigated. Read More

View Article and Full-Text PDF

The Mutual Influence of Predominant Microbes in Sourdough Fermentation: Focusing on Flavor Formation and Gene Transcription.

Foods 2022 Aug 8;11(15). Epub 2022 Aug 8.

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

The interplay between microorganisms generally plays a vital role in food fermentation. In this study, the mutual influence of and the two predominant microbes in the sourdough ecosystem, were investigated in situ during fermentation. Doughs fermented with , or their combination were compared regarding acid production, microbial density, and volatiles. Read More

View Article and Full-Text PDF

A Minimally Invasive Approach for Preventing White Wine Protein Haze by Early Enzymatic Treatment.

Foods 2022 Jul 28;11(15). Epub 2022 Jul 28.

Department of Agriculture and Forest Sciences (DAFNE), Tuscia University, Via S. Camillo de Lellis snc, 01100 Viterbo, Italy.

Protein stability in bottled white wine is an essential organoleptic property considered by consumers. In this paper, the effectiveness of an early enzymatic treatment was investigated by adding a food-grade microbial protease at two different stages of winemaking: (i) at cold settling, for a short-term and low temperature (10 °C) action prior to alcoholic fermentation (AF); (ii) at yeast inoculum, for a long-lasting and medium temperature (18 °C) action during AF. The results reveal that protease sufficiently preserved its catalytic activity at both operational conditions: 10 °C (during cold settling) and 18 °C (during AF). Read More

View Article and Full-Text PDF

Active and Intelligent Packaging for Enhancing Modified Atmospheres and Monitoring Quality and Shelf Life of Packed Gilthead Seabream Fillets at Isothermal and Variable Temperature Conditions.

Foods 2022 Jul 27;11(15). Epub 2022 Jul 27.

Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens (NTUA), 15780 Athens, Greece.

The study investigated the effect of active modified atmosphere packaging (20% CO-60% N-20% O) with CO emitters (MAP-PAD) and conventional MAP (MAP) on the quality and shelf-life of gilthead seabream fillets during chill storage, while the most appropriate enzymatic Time Temperature Integrators (TTI) were selected for monitoring their shelf-life at isothermal and variable temperature storage conditions ( = 4.8 °C). The concentration of CO and O in the headspace of the package, volatile compounds and of the microbial population were monitored during storage. Read More

View Article and Full-Text PDF

Screening of Microbial Fermentation Products for Anti- Activity.

Animals (Basel) 2022 Jul 31;12(15). Epub 2022 Jul 31.

State Key Laboratory Breeding Base for The Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China.

Tuberculosis (TB), caused by , is the leading infectious cause of mortality worldwide. The emergence of drug-resistant has made the control of TB more difficult. In our study, we investigated the ability of microorganism fermentation products from the soil to inhibit . Read More

View Article and Full-Text PDF

The effect of ensiled paulownia leaves in a high-forage diet on ruminal fermentation, methane production, fatty acid composition, and milk production performance of dairy cows.

J Anim Sci Biotechnol 2022 Aug 12;13(1):104. Epub 2022 Aug 12.

Department of Animal Nutrition, Poznań University of Life Sciences, Wołyńska 33, 60-637, Poznań, Poland.

Background: The use of industrial by-products rich in bioactive compounds as animal feeds can reduce greenhouse gas production. Paulownia leaves silage (PLS) was supplemented to dairy cows' diet and evaluated in vitro (Exp. 1; Rusitec) and in vivo (Exp. Read More

View Article and Full-Text PDF

A study on the antibacterial activity and antimicrobial resistance of pyridinium cationic pillar[5]arene against Staphylococcus aureus and Escherichia coli.

Int Microbiol 2022 Aug 12. Epub 2022 Aug 12.

College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, People's Republic of China.

An increasing number of infections caused by multidrug-resistant (MDR) Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) have severely affected human society. Read More

View Article and Full-Text PDF

ZBTB46 in ILC3: shared transcriptional infrastructure defines gut-protective capabilities.

Trends Immunol 2022 Aug 8. Epub 2022 Aug 8.

The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia. Electronic address:

Group 3 innate lymphoid cells (ILC3s) are distributed along the gastrointestinal tract at the interface between the immune system and the gut lumen, which carries a significant microbial burden. In a new study, Zhou et al. investigated the expression of transcription factor ZBTB46, normally thought to be restricted to classical dendritic cells (cDCs), and discovered that ZBTB46 expression by ILC3s in the mouse colon forms an essential part of the gastrointestinal armory to calibrate inflammatory responses. Read More

View Article and Full-Text PDF

Molecular approaches to uncover phage-lactic acid bacteria interactions in a model community simulating fermented beverages.

Food Microbiol 2022 Oct 9;107:104069. Epub 2022 Jun 9.

Normandie Univ, UNICAEN, UNIROUEN, ABTE, 14000, Caen, France. Electronic address:

Food microbial diversity and fluxes during the fermentation processes are well studied whereas phages-bacteria interactions are still poorly described in the literature. This is especially true in fermented beverages, and especially in cider, which is an alcoholic fermented apple beverage. The transcriptomic and proteomic responses of the lactic acid bacterium (LAB) Liquorilactobacillus mali UCMA 16447 to a lytic infection by phage UCMA 21115, both isolated from cider, were investigated, in order to get a better understanding of phages-bacteria interactions in such fermented beverage. Read More

View Article and Full-Text PDF
October 2022

Spoilage potential of bacterial species from chilled vacuum-packed lamb.

Food Microbiol 2022 Oct 16;107:104093. Epub 2022 Jul 16.

Centre for Food Safety and Innovation, Tasmanian Institute of Agriculture, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia.

The objective of this study was to establish whether specific organisms play important roles in the spoilage rate of vacuum-packed (VP) lamb at low storage temperatures. The spoilage potential of representative organisms (n = 13) of the spoilage community of VP lamb were investigated through a series of shelf-life challenge trials. Each isolate was individually inoculated onto sterile (irradiated) and non-sterile (i. Read More

View Article and Full-Text PDF
October 2022

Biochar facilitated bacterial reduction of Cr(VI) by Shewanella Putrefaciens CN32: Pathways and surface characteristics.

Environ Res 2022 Aug 8:113971. Epub 2022 Aug 8.

State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. Electronic address:

Biochar can facilitate the microbial reduction of various pollutants in soil and groundwater environments, but its impact on Cr(VI) reduction by dissimilatory metal reducing bacteria (DMRB) remains to be systematically investigated. In this study, we prepared biochars at 500 °C and 700 °C from wheat straw and grass, and investigated the impact of these biochars on Cr(VI) reduction by a model DMRB, Shewanella Putrefaciens CN32 (CN32). Pristine biochars abiotically reduced Cr(VI), which decreased the concentration and toxicity of chromium to CN32 cells, and brought about higher overall Cr(VI) removal extent after CN32 were added sequentially; on the other hand, no enhancement effect were observed when biochars and CN32 were added simultaneously. Read More

View Article and Full-Text PDF

Cuprous Oxide Nanoparticles Decorated Fabric Materials with Anti-biofilm Properties.

ACS Appl Bio Mater 2022 Aug 11. Epub 2022 Aug 11.

Department of Chemistry, Faculty of Exact Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

Considering the global spread of bacterial infections, the development of anti-biofilm surfaces with high antimicrobial activities is highly desired. This work unraveled a simple, sonochemical method for coating CuO nanoparticles (NPs) on three different flexible substrates: polyester (PE), nylon 2 (N2), and polyethylene (PEL). The introduction of CuO NPs on these substrates enhanced their surface hydrophobicity, induced ROS generation, and completely inhibited the growth of sensitive ( and ) and drug-resistant (MDR and MRSA) planktonic and biofilm. Read More

View Article and Full-Text PDF

Bioaccessibility of Microplastic-Associated Antibiotics in Freshwater Organisms: Highlighting the Impacts of Biofilm Colonization an Protocol.

Environ Sci Technol 2022 Aug 11. Epub 2022 Aug 11.

College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China.

Microplastics in the environment can be colonized by microbes capable of forming biofilms, which may act as reactive coatings to affect the bioaccessibility of pollutants in organisms. This study investigated the dynamic evolution of biofilm colonization on microplastics and its impacts and mechanisms on the bioaccessibility of microplastic-associated sulfamethazine (SMT) microcosm incubation in surface water and sediment. After 60 days of incubation, the microbial communities formed in microplastics were distinct and more diverse than those untethered in surroundings, and photoaging treatment decreased the affinity of biofilms on microplastics due to decreased hydrophobicity. Read More

View Article and Full-Text PDF

Suppression of trimethylamine N-oxide with DMB mitigates vascular dysfunction, exercise intolerance, and frailty associated with a Western-style diet in mice.

J Appl Physiol (1985) 2022 Aug 11. Epub 2022 Aug 11.

Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United States.

Consumption of a Western-style diet (WD; high fat, high sugar, low fiber) is associated with impaired vascular function and increased risk of cardiovascular diseases (CVD), which could be mediated partly by increased circulating concentrations of the gut microbiome-derived metabolite trimethylamine N-oxide (TMAO). We investigated if suppression of TMAO with 3,3-dimethyl-1-butanol (DMB; inhibitor of microbial TMA lyase) in mice could prevent: 1) WD-induced vascular endothelial dysfunction and aortic stiffening; and 2) WD-induced reductions in endurance exercise tolerance and increases in frailty, as both are linked to WD, vascular dysfunction, and increased CVD risk. C57BL/6N mice were fed standard chow or WD (41% fat, ~25% sugar, 4% fiber) for 5 months beginning at ~2 months of age. Read More

View Article and Full-Text PDF

Insights into the human gut virome by sampling a population from the Indian subcontinent.

J Gen Virol 2022 Aug;103(8)

Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad-Gurugram Expressway, Faridabad-121 001, Haryana, India.

Gut virome plays an important role in human physiology but remains poorly understood. This study reports an investigation of the human gut DNA-virome of a previously unexplored ethnic population through metagenomics of faecal samples collected from individuals residing in Northern India. Analysis shows that, similar to the populations investigated earlier, majority of the identified virome belongs to bacteriophages and a smaller fraction (<20 %) consists of viruses that infect animals, archaea, protists, multiple domains or plants. Read More

View Article and Full-Text PDF

Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field.

Appl Environ Microbiol 2022 Aug 11:e0092922. Epub 2022 Aug 11.

School of the Earth, Ocean, and Environment, University of South Carolina, Columbia, South Carolina, USA.

Alkaline fluids venting from chimneys of the Lost City hydrothermal field flow from a potentially vast microbial habitat within the seafloor where energy and organic molecules are released by chemical reactions within rocks uplifted from Earth's mantle. In this study, we investigated hydrothermal fluids venting from Lost City chimneys as windows into subseafloor environments where the products of geochemical reactions, such as molecular hydrogen (H), formate, and methane, may be the only available sources of energy for biological activity. Our deep sequencing of metagenomes and metatranscriptomes from these hydrothermal fluids revealed a few key species of archaea and bacteria that are likely to play critical roles in the subseafloor microbial ecosystem. Read More

View Article and Full-Text PDF

Gastrointestinal Biogeography of Luminal Microbiota and Short-Chain Fatty Acids in Sika Deer (Cervus nippon).

Appl Environ Microbiol 2022 Aug 11:e0049922. Epub 2022 Aug 11.

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.

The gut microbiota of sika deer has been widely investigated, but the spatial distribution of symbiotic microbes among physical niches in the gastrointestinal tract remains to be established. While feces are the most commonly used biological samples in these studies, the accuracy of fecal matter as a proxy of the microbiome at other gastrointestinal sites is as yet unknown. In the present study, luminal contents obtained along the longitudinal axis of deer gastrointestinal tract (rumen, reticulum, omasum, abomasum, small intestine, cecum, colon, and rectum) were subjected to 16S rRNA gene sequencing for profiling of the microbial composition, and samples from the rumen, small intestine, and cecum were subjected to metabolomic analysis to evaluate short-chain fatty acid (SCFA) profiles. Read More

View Article and Full-Text PDF

Propionate stimulates the secretion of satiety hormones and reduces acute appetite in a cecal fistula pig model.

Anim Nutr 2022 Sep 17;10:390-398. Epub 2022 Jun 17.

Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Short-chain fatty acids (SCFA) can regulate appetite by stimulating the secretion of satiety hormones. However, the impact of short-chain fatty acid propionate on the release of gut satiety hormones and appetite regulation in pigs is not completely understood. In this study, 16 pigs were infused with saline or sodium propionate through a fistula in the caecum during a 28-day experimental period. Read More

View Article and Full-Text PDF
September 2022

Combining high pressure homogenization with free nitrous acid pretreatment to improve anaerobic digestion of sewage sludge.

J Environ Manage 2022 Sep 1;318:115635. Epub 2022 Jul 1.

Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China. Electronic address:

Single pretreatment of sewage sludge, either physical, chemical or biological, has its own drawbacks in term of poor sanitization, energy intensity and high operational and capital cost. To tackle these drawbacks, combined high pressure homogenization (HPH) and free nitrous acid (FNA) pretreatment for sludge solubilization and further biodegradation in anaerobic digestion was investigated. Synergistic effect of combined HPH (40 MPa) and FNA (2. Read More

View Article and Full-Text PDF
September 2022