2,747 results match your criteria investigated genotoxicity

Disruption of postnatal neurogenesis and adult-stage suppression of synaptic plasticity in the hippocampal dentate gyrus after developmental exposure to sterigmatocystin in rats.

Toxicol Lett 2021 Jun 11. Epub 2021 Jun 11.

Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan. Electronic address:

Exposure to sterigmatocystin (STC) raises concerns on developmental neurological disorders. The present study investigated the effects of maternal oral STC exposure on postnatal hippocampal neurogenesis of offspring in rats. Dams were exposed to STC (1. Read More

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Spectroscopic and Simulation Studies of the Sequence-Dependent DNA Destabilization by a Fungicide.

ACS Omega 2021 Jun 26;6(22):14371-14378. Epub 2021 May 26.

School of Chemical Sciences, Indian Association for the Cultivation of Science, Kolkata 700032, India.

The understanding of the structural change of DNA induced by fungicides is essential as the non-targeted action of fungicides causes genotoxicity, leading to several serious diseases such as cancer, behavioral change, and nausea. In this study, the binding of an important fungicide, namely, -dodecylguanidine acetate (dodine), with B-DNA having different sequences of nucleobases and its effect on the structure of B-DNA has been investigated using spectroscopic and simulation methods. In general, the addition of dodine destabilizes DNA; however, the binding of dodine causing the destabilization of DNA is highly sequence dependent. Read More

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Ameliorative role of ascorbic acid on the oxidative stress and genotoxicity induced by acetamiprid in Nile tilapia (Oreochromis niloticus).

Environ Sci Pollut Res Int 2021 Jun 14. Epub 2021 Jun 14.

Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt.

On juveniles of Oreochromis niloticus, the protective potential of ascorbic acid (Asc) against oxidative stress and genotoxicity induced by acetamiprid (Aceta) sub-lethal concentrations was investigated in this study. Fishes were divided into six groups and exposed to either Asc (50 ppm), 10 and 20 ppm Aceta, 10 ppm (Aceta)+Asc, 20 ppm (Aceta)+Asc, or the unexposed control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and their transcripts were assessed. Read More

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Support for Regulatory Assessment of Percutaneous Absorption of Retronecine-type Pyrrolizidine Alkaloids through Human Skin.

Planta Med 2021 Jun 11. Epub 2021 Jun 11.

Procter & Gamble Technical Centres Ltd, Reading, UK.

1,2-unsaturated pyrrolizidine alkaloids are found naturally in , well known as comfrey, which has a longstanding use for the topical treatment of painful muscle and joint complaints. Pyrrolizidine alkaloids (PA) are a relevant concern for the safety assessment due to their liver genotoxicity profile, and close attention is paid during manufacturing to minimizing their levels. Current regulatory risk assessment approaches include setting limits that derive from toxicity data coming from the oral route of exposure. Read More

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Investigation of genotoxic effects of rhododendron honey using three mammalian bioassays .

Drug Chem Toxicol 2021 Jun 8:1-10. Epub 2021 Jun 8.

Duzce Vocational School, Duzce University, Duzce, Turkey.

Rhododendron honey (RH) is obtained from the rhododendron plants are grown in many regions around the world, causes poisoning in humans due to the grayanotoxin (GTX) compound in its structure. It is used by the public as a therapeutic for some diseases. It was aimed to study the genotoxic and cytotoxic effects of RH in mouse bone-marrow and sperm cells by using three mammalian bioassays. Read More

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Biotransformation of Reactive Red 141 by KKW2-005 and examination of product toxicity.

J Microbiol Biotechnol 2021 Jun 1. Epub 2021 Jun 1.

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Klong Luang, Pathum Thani, Thailand, 12120.

A total of 37 bacterial isolates were obtained from dye contaminated soil samples at a textile processing factory in Nakhon Ratchasima province, Thailand. The potential of decolourisation and biotransformation of azo dye reactive red 141 (RR141) were investigated. The most potent bacterium was identified as KKW2-005 and achieved capable of decolourising 96. Read More

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Molecular docking and toxicity assessment of spirodiclofen: protective role of lycopene.

Environ Sci Pollut Res Int 2021 Jun 5. Epub 2021 Jun 5.

Faculty of Medicine, Department of Pharmacology, Giresun University, Giresun, Turkey.

In this study, toxic effects of spirodiclofen and protective role of lycopene against toxic effects were investigated by using physiological, cytogenetic, anatomical, and biochemical parameters. Allium cepa L. bulbs were used as test material. Read More

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N-vinyl compounds: studies on metabolism, genotoxicity, carcinogenicity.

Arch Toxicol 2021 Jun 6. Epub 2021 Jun 6.

BASF SE, Experimental Toxicology and Ecology, 67056, Ludwigshafen am Rhein, Germany.

Several N-vinyl compounds are produced in high volumes and are widely employed in the production of copolymers and polymers used in chemical, pharmaceutical, cosmetic and food industry. Hence, information on their genotoxicity and carcinogenicity is requisite. This review presents hitherto available information on the carcinogenicity and genotoxicity of N-vinyl compounds as well as their metabolism potentially generating genotoxic and carcinogenic derivatives. Read More

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Effects of Acrylamide-Induced Vasorelaxation and Neuromuscular Blockage: A Rodent Study.

Toxics 2021 May 24;9(6). Epub 2021 May 24.

Graduate Institute of Food Safety, National Chung Hsing University, Taichung 402, Taiwan.

Acrylamide (ACR), which is formed during the Maillard reaction, is used in various industrial processes. ACR accumulation in humans and laboratory animals results in genotoxicity, carcinogenicity, neurotoxicity, and reproductive toxicity. In this study, we investigated the mechanisms by which ACR may induce vasorelaxation and neuromuscular toxicity. Read More

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Growth Inhibition and Aflatoxin B Decontamination by Isolates and Their Metabolites.

Toxins (Basel) 2021 05 8;13(5). Epub 2021 May 8.

UMR Qualisud, University of Montpellier, 34095 Montpellier, France.

Aflatoxin B is a potent carcinogen produced by , mainly during grain storage. As pre-harvest methods are insufficient to avoid mycotoxin presence during storage, diverse curative techniques are being investigated for the inhibition of fungal growth and aflatoxin detoxification. spp. Read More

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Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application.

J Funct Biomater 2021 May 20;12(2). Epub 2021 May 20.

Department of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland.

Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5'-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Read More

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Cr(VI)-induced DNA damage is lessened by the modulation of hsp70 via increased GSH de novo synthesis in Drosophila melanogaster.

J Biochem Mol Toxicol 2021 May 30:e22819. Epub 2021 May 30.

Embryotoxicology Laboratory, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, Uttar Pradesh, India.

Hexavalent chromium [Cr(VI)] is a genotoxic chemical, and in the chemical-exposed organism, oxidative stress is one of the leading causative mechanisms of genotoxicity. Heat shock protein-70 (Hsp70) is reported to be modulated in environmental chemical exposed organisms. Inadequate information on the protective role of Hsp70 in chemical-induced DNA lesions prompted us to investigate this possibility in a well-studied genetically tractable in vivo model Drosophila melanogaster. Read More

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Reno-Hepatoprotective and Antidiabetic Properties of Methanol Leaf Extract of in Wistar Rats.

J Evid Based Integr Med 2021 Jan-Dec;26:2515690X211017464

Department of Biochemistry, Cancer Research and Molecular Biology Laboratories, 58987University of Ibadan, Oyo, Nigeria.

Toxicities due to exposure to arsenic-contaminated water and the occurrence of diabetes mellitus are major health concerns. Treatment of these concerns using therapeutic measures have recorded limited success. Traditionally, (LA) has been used in managing various diseases. Read More

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Plinia cauliflora (Mart.) Kausel (Jaboticaba) leaf extract: In vitro anti-Trypanosoma cruzi activity, toxicity assessment and phenolic-targeted UPLC-MS metabolomic analysis.

J Ethnopharmacol 2021 May 24;277:114217. Epub 2021 May 24.

Laboratory of Environmental Mutagenesis, Department of Biophysics and Biometry, Rio de Janeiro State University, Rio de Janeiro, Brazil.

Ethnopharmacological Relevance: Plinia cauliflora (Mart.) Kausel, known as Brazilian grape or jaboticaba, is widely used in Brazilian traditional medicine to treat infectious and inflammatory disorders. However, several aspects of its biological potential remain unclear, such as toxicity and effects on pathogenic protozoa. Read More

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Mass spectrometry investigation of nucleoside adducts of fatty acid hydroperoxides from oxidation of linolenic and linoleic acids.

J Chromatogr A 2021 Jul 11;1649:462236. Epub 2021 May 11.

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China. Electronic address:

The widespread presence of lipid hydroperoxides in foodstuffs and biological samples has aroused great attentions in recent years, while it remains challenging for analysis of the fragility of O - O bond linkage of peroxides. In this present study, we explored the utility of electrospray ionization mass spectrometry (ESI-MS) for characterization of two fatty acid hydroperoxides from oxidation of linoleic acid and α-linolenic acid, which are the essential fatty acids abundant in many seeds and vegetable oils. The results indicated that in-source fragmentation occurred in the detection of the two fatty acid hydroperoxides in both positive and negative ion modes, which yielded characteristic fragments for ESI-MS analysis. Read More

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Unburned tobacco cigarette smoke alters rat ultrastructural lung airways and DNA.

Nicotine Tob Res 2021 May 24. Epub 2021 May 24.

Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Via Irnerio, Bologna, Italy.

Introduction: Recently, the Food and Drug Administration (FDA) authorized the marketing of IQOS Tobacco Heating System as a Modified Risk Tobacco Product (MRTP) based on an electronic heat-not-burn technology that purports to reduce the risk.

Methods: Sprague-Dawley rats were exposed in a whole-body mode to IQOS aerosol for 4 weeks. We performed the chemical characterization of IQOS mainstream and we studied the ultrastructural changes in trachea and lung parenchyma of rats exposed to IQOS stick mainstream and tissue pro-inflammatory markers. Read More

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Cytotoxicity and genotoxicity of coated-gold nanoparticles on freshwater algae Pseudokirchneriella subcapitata.

Aquat Toxicol 2021 Jul 14;236:105865. Epub 2021 May 14.

Emerging Contaminants Ecological and Risk Assessment (ECERA) Research Group, Department of Chemical Engineering, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa. Electronic address:

Gold engineered nanoparticles (nAu) are increasingly detected in ecosystems, and this raises the need to establish their potential effects on aquatic organisms. Herein, cytotoxic and genotoxic effects of branched polyethylenimine (BPEI)- and citrate (cit)-coated nAu (5, 20, and 40 nm) on algae Pseudokirchneriella subcapitata were evaluated. The apical biological endpoints: growth inhibition and chlorophyll a (Chl a) content were investigated at 62. Read More

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Chronic and transgenerational effects of polystyrene microplastics at environmentally relevant concentrations on earthworms (Eisenia fetida).

Environ Toxicol Chem 2021 May 25. Epub 2021 May 25.

Global Centre for Environmental Remediation (GCER), University of Newcastle, College of Engineering, Science and Environment, Callaghan, NSW, 2308, Australia.

Recently, soil contamination with microplastics has emerged as a serious global environmental concern which necessitated more research on their potential impacts on soil biota. In this study, we investigated the acute and chronic toxicity of two different polystyrene (PS) microplastics, pure vs commercial (0-0.5% w/w in soil), with the sizes of 65-125 µm on the earthworm mortality, reproduction and genotoxicity. Read More

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Chemical composition of Callistemon citrinus (Curtis) Skeels aerial part essential oil and its pharmacological applications, neurodegenerative inhibitory, and genotoxic efficiencies.

J Food Biochem 2021 May 24:e13767. Epub 2021 May 24.

Medicinal Aromatic and Economic Plants Group, Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (NEIST), Jorhat, Assam, India.

Callistemon citrinus aerial part essential oil (CCEO) was analyzed for chemical compositions using GC/MS. Pharmacological activities such as neurodegenerative inhibitory, antioxidant, anti-inflammatory, antimicrobial, and acetylcholinesterase activities were evaluated using the standard methodologies. Genotoxicity was investigated using Allium cepa assay. Read More

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Biodistribution and safety studies of a bicistronic plasmid for nerve repair.

Tissue Eng Part C Methods 2021 May 20. Epub 2021 May 20.

Moscow State University, 64935, Medical Research and Education Center, Moscow, Russian Federation;

Background: Gene therapy is one of the promising approaches for regenerative medicine. Local and long-term expression of essential growth factors allows to achieve the desired therapeutic effect. However, some aspects of prolonged usage of genetic constructs encoding growth factors, such as toxicity, mutagenicity, genotoxicity and ability to disseminate from the injection site and mediate ectopic expression of therapeutic proteins, are poorly investigated. Read More

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Protective effects of apigenin against edifenphos-induced genotoxicity and cytotoxicity in rat hepatocytes.

J Biomol Struct Dyn 2021 May 17:1-16. Epub 2021 May 17.

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

Edifenphos (EDF) is an organophosphorus pesticide with antifungal and anti-insecticidal properties. However, EDF accumulates in various agricultural products and causes potential hazards to human health. Although numerous reports have indicated EDF accumulation in agricultural products, toxic effects on cellular system is poorly understood. Read More

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Propolis loaded and genipin-crosslinked PVA/chitosan membranes; characterization properties and cytocompatibility/genotoxicity response for wound dressing applications.

Seda Ceylan

Int J Biol Macromol 2021 Jun 12;181:1196-1206. Epub 2021 May 12.

Bioengineering Department, Faculty of Engineering, Adana Alparslan Türkeş Science and Technology University, Adana, Turkey. Electronic address:

Loading propolis by a simple process using genipin as a crosslinking agent and fabrication of a novel PVA/Chitosan-Propolis membrane scaffolds were reported for wound dressing applications. The research is focused on the effects of propolis on characterization properties of membrane such as chemical structure, surface morphology, degradation ratio, crystallinity, hydrophilicity, water uptake capacity, water vapour transmission rate and mechanical aspect. It was noticed that water uptake capacity and hydrophilicity properties of membrane considerably affected by the propolis. Read More

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Radiobiological risks following dentomaxillofacial imaging: should we be concerned?

Dentomaxillofac Radiol 2021 May 14:20210153. Epub 2021 May 14.

Belgian Nuclear Research Centre (SCK CEN), Radiobiology Unit, Boeretang 200, Mol, Belgium.

Objectives: This review aimed to present studies that prospectively investigated biological effects in patients following diagnostic dentomaxillofacial radiology (DMFR).

Methods: Literature was systematically searched to retrieve all studies assessing radiobiological effects of using X-ray imaging in the dentomaxillofacial area, with reference to radiobiological outcomes for other imaging modalities and fields.

Results: There is a lot of variability in the reported radiobiological assessment methods and radiation dose measures, making comparisons of radiobiological studies challenging. Read More

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Nanoplastic-Induced Genotoxicity and Intestinal Damage in Freshwater Benthic Clams (): Comparison with Microplastics.

ACS Nano 2021 May 14. Epub 2021 May 14.

The Key Laboratory of Water and Sediment Sciences, Ministry of Education, School of Environment, Beijing Normal University, Beijing 100875, P.R. China.

With the wide application of plastics in daily life, nanoplastics (NPs) are ubiquitous in freshwater environments. However, to date, few studies have focused on the mechanism underlying the toxicity of NPs, and the differences between this mechanism and that governing the toxicity of MPs have also not been thoroughly characterized. In this study, the genotoxicity, intestinal damage, and intestinal flora in exposed to micro/nanoplastics were investigated through RNA sequencing, histopathology, and 16S rRNA sequencing, respectively. Read More

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Differential effects of cisplatin combined with the flavonoid apigenin on HepG2, Hep3B, and Huh7 liver cancer cell lines.

Mutat Res 2021 Jun 24;866:503352. Epub 2021 Mar 24.

Laboratory of Experimental Surgery and Surgical Research, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, 68 100, Greece; Postgraduate Program in Hepatobiliary and Pancreatic Surgery, 2nd Department of Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, 68 100, Greece.

The potential of apigenin (APG) to enhance cisplatin's (CDDP) chemotherapeutic efficacy was investigated in HepG2, Hep3B, and Huh7 liver cancer cell lines. The presence of 20 μM APG sensitized all cell lines to CDDP treatment (degree of sensitization based on the MTT assay: HepG2>Huh7>Hep3B). As reflected by sister chromatid exchange levels, the degree of genetic instability as well as DNA repair by homologous recombination differed among cell lines. Read More

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Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor.

Food Chem Toxicol 2021 Jul 10;153:112258. Epub 2021 May 10.

Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, ENVT, INP-Purpan, UPS, 31027, Toulouse, France.

Aflatoxins are a group of mycotoxins that have major adverse effects on human health. Aflatoxin B1 (AFB1) is the most important aflatoxin and a potent carcinogen once converted into a DNA-reactive form by cytochrome P450 enzymes (CYP450). AFB1 biosynthesis involves the formation of Versicolorin A (VerA) which shares structural similarities with AFB1 and can be found in contaminated commodities, often co-occurring with AFB1. Read More

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The screening of the safety profile of polymeric based amoxicillin nanoparticles in various test systems.

Toxicol Lett 2021 May 10;348:1-9. Epub 2021 May 10.

Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Istanbul, Turkey. Electronic address:

Nanotechnology-based drugs show superiority over conventional medicines because of increased bioavailability, lower accumulation in non-target tissues, and improved therapeutic index with increased accumulation at target sites. However, it is important to be aware of possible problems related to the toxicity of these products, which have therapeutically superior properties. Accordingly, the present study was designed to investigate the safety profile of amoxicillin nanoparticles (AmxNPs) that we developed to increase the oral bioavailability of amoxicillin (Amx) in poultry. Read More

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A harmonized and standardized in vitro approach produces reliable results on silver nanoparticles toxicity in different cell lines.

J Appl Toxicol 2021 May 12. Epub 2021 May 12.

Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy.

Despite the widespread use of silver nanoparticles (AgNPs) in different fields and the amount of investigations available, to date, there are many contradictory results on their potential toxicity. In the present study, extensively characterized 20-nm AgNPs were investigated using optimized protocols and standardized methods to test several toxicological endpoints in different cell lines. The agglomeration/aggregation state of AgNPs in culture media was measured by dynamic light scattering (DLS). Read More

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Acute toxicity analysis of an inhibitor of BCL2, Disarib, in rats.

Sci Rep 2021 May 11;11(1):9982. Epub 2021 May 11.

Institute of Bioinformatics and Applied Biotechnology, Electronics City, Bangalore, 560100, India.

Apoptosis or programmed cell death is a highly regulated process, which eliminates unwanted and damaged cells. Inhibition of apoptosis is a hallmark of cancer cells. BCL2 family proteins are known to play a vital role in the regulation of apoptosis. Read More

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In vitro biochemical characterization and genotoxicity assessment of Sapindus saponaria seed extract.

J Ethnopharmacol 2021 Aug 28;276:114170. Epub 2021 Apr 28.

Laboratory of Biochemistry and Function of Plant Proteins, Research Center on Agricultural Systems, Department of Botany, Federal University of the State of Rio de Janeiro, Av. Pasteur, 458, Urca, Brazil. Electronic address:

Ethnopharmacological Relevance: Sapindus saponaria, also popularly known as soapberry, has been used in folk medicinal values because of its therapeutic properties and several compounds in its composition, which represent a target in potential for drug discovery. However, few data about its potential toxicity has been reported.

Aim Of The Study: Plant proteins can perform essential roles in survival, acting as defense mechanism, as well functioning as important molecular reserves for its natural metabolism. Read More

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