54,529 results match your criteria investigate cultural

[Bax inhibitor-1 inhibits calcification of vascular smooth muscle cells ].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Aug;41(8):1177-1182

Department of Cardiology, First Medical Center, General Hospital of PLA, Beijing 100853, China.

Objective: To investigate the effect of Bax inhibitor-1(BI-1)on calcification of vascular smooth muscle cells(VSMCs).

Methods: VSMCs were isolated from the thoracic aorta of SD rats.VSMCs or BI-1-overexpressing VSMCs(transfected with a BI-1-overexpressing plasmid) were cultured in normal medium or calcified medium containing β-glycerophosphate and calcium chloride, and the cell calcification was examined with Alizarin red staining. Read More

View Article and Full-Text PDF

[Modulatory effect of 2-arachidonoylglycerol on voltage-gated sodium currents in rat caudate nucleus neurons with kainic acid-induced injury].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Aug;41(8):1150-1157

Department of Functional Sciences, College of Medical Science, China Three Gorges University, Yichang 443002, China.

Objective: To investigate the modulatory effect of 2-arachidonoylglycerol (2-AG) on voltage-gated sodium currents(VGSCs) in rat caudate nucleus (CN) neurons with kainic acid (KA)-induced injury and explore the molecular mechanism underlying the neuroprotective effect of 2-AG.

Methods: Primary cultures of CN neurons isolated from neonatal SD rats were treated with KA, 2-AG+KA, RIM (a CB1 receptor antagonist) +2-AG+KA, or vehicle only (as control).After 7 days in primary culture, the neurons were treated with corresponding agents for 12 h (RIM and 2-AG were added at the same time; KA was added 30 min later) before recording of current density changes, current-voltage characteristics, activation and inactivation kinetics of VGSCs () using whole-cell patch clamp technique. Read More

View Article and Full-Text PDF

[Mechanism of high expression of high mobility group protein 1 in a rat model of knee osteoarthritis].

W Mei B Hong G Huang

Nan Fang Yi Ke Da Xue Xue Bao 2021 Aug;41(8):1142-1149

Department of Orthopedics and Traumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, China.

Objective: To investigate the molecular mechanism triggering pyroptosis of synovial fibroblast-like synoviocytes(FLSs)and the release of high mobility group protein 1(HMGB1)in a rat model of knee osteoarthritis(KOA).

Methods: Twelve SD rats were randomized equally into blank control group without any treatment and KOA group with anterior cruciate ligament amputation (ACLT) to induce KOA.HE staining and Mankin score were used to evaluate the damage of knee cartilage. Read More

View Article and Full-Text PDF

Impact of COVID-19 partial lockdown on household activities in a sample of Kuwaiti and Saudi populations.

Int Soc Sci J 2021 Jul 1. Epub 2021 Jul 1.

Social Work at Sociology and Social Work Department College of Social Science, Kuwait University.

Since the World Health Organization (WHO) declared COVID-19 a global pandemic, partial lockdown has been used as a strict mechanism to constrain the spread of the disease. This study aims to investigate whether there are significant differences between socio-demographic variables and household and family activities before and during the partial lockdown in Kuwait and the Kingdom of Saudi Arabia due to the COVID-19 pandemic. A descriptive cross-sectional method was used in this study. Read More

View Article and Full-Text PDF

Single-cell DNA and RNA sequencing reveals the dynamics of intra-tumor heterogeneity in a colorectal cancer model.

BMC Biol 2021 Sep 21;19(1):207. Epub 2021 Sep 21.

Division of Bioinformatics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan.

Background: Intra-tumor heterogeneity (ITH) encompasses cellular differences in tumors and is related to clinical outcomes such as drug resistance. However, little is known about the dynamics of ITH, owing to the lack of time-series analysis at the single-cell level. Mouse models that recapitulate cancer development are useful for controlled serial time sampling. Read More

View Article and Full-Text PDF
September 2021

Microbiota composition and susceptibility to florfenicol and oxytetracycline of bacterial isolates from mussels (Mytilus spp.) reared on different years and distance from salmon farms.

Environ Res 2021 Sep 18:112068. Epub 2021 Sep 18.

Laboratory of Microbiology and Probiotics, Institute of Nutrition and Food Technology (INTA), University of Chile, Chile; ANID - Millennium Science Initiative Program - Millennium Nucleus in the Biology of the Intestinal Microbiota, Chile. Electronic address:

Chilean aquaculture mainly produces salmonids and molluscs. Salmonid production has been questioned by its excessive use of antimicrobials. This study aimed to investigate the bacterial microbiota composition of Mytilus spp. Read More

View Article and Full-Text PDF
September 2021

ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.

Cancer Biol Med 2021 Sep 21. Epub 2021 Sep 21.

National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan.

Objective: The aim of the present study was to investigate the mechanisms responsible for the radiation-sensitizing effect of antennapedia proteins, ANTP-SMACN7, on lung cancer cells treated with accelerated carbon and Fe particle irradiation.

Methods: The ANTP-SMACN7 fusion peptide was synthesized and linked to fluorescein isothiocyanate to determine its ability to penetrate cells. A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Read More

View Article and Full-Text PDF
September 2021

The role of the adipose tissue-derived mesenchymal stem cells enriched with melatonin on pancreatic cellular regeneration.

Folia Morphol (Warsz) 2021 Sep 21. Epub 2021 Sep 21.

Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt.

Background: Adipose tissue-derived mesenchymal stem cells (AD-MSCs) were proved to differentiate into insulin-producing cells (IPCs), but the amount of insulin secreted was relatively low compared to the insulin secreted by mature pancreatic islets. Enrichment of MSCs culture with melatonin (MT) was detected to promote cartilage matrix synthesis, osteogenic and neuronal differentiation. Therefore, the present study was conducted to evaluate the potential role of MT pre-treated AD-MSCs in enhancing the treatment and regeneration of the islet cells of Langerhans in diabetic rats induced by streptozotocin (STZ). Read More

View Article and Full-Text PDF
September 2021

A dynamic microscale mid-throughput fibrosis model to investigate the effects of different ratios of cardiomyocytes and fibroblasts.

Lab Chip 2021 Sep 21. Epub 2021 Sep 21.

Departments of Biomedicine and Surgery, University Basel and University Hospital Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland.

Cardiac fibrosis is a maladaptive remodeling of the myocardium hallmarked by contraction impairment and excessive extracellular matrix deposition (ECM). The disease progression, nevertheless, remains poorly understood and present treatments are not capable of controlling the scarring process. This is partly due to the absence of physiologically relevant, easily operable, and low-cost models, which are of the utmost importance to uncover pathological mechanisms and highlight possible targets for anti-fibrotic therapies. Read More

View Article and Full-Text PDF
September 2021

Enhancement of pyocyanin production by subinhibitory concentration of royal jelly in .

F1000Res 2021 11;10:14. Epub 2021 Jan 11.

Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281, Indonesia.

, a multidrug-resistant Gram-negative bacterium, produces pyocyanin, a virulence factor associated with antibiotic tolerance. High concentrations of royal jelly have an antibacterial effect, which may potentially overcome antibacterial resistance. However, in some cases, antibiotic tolerance can occur due to prolonged stress of low-dose antibacterial agents. Read More

View Article and Full-Text PDF
January 2021

Prediction of Hepatic Drug Clearance with a Human Microfluidic Four-Cell Liver Acinus MicroPhysiology System.

Toxicology 2021 Sep 17:152954. Epub 2021 Sep 17.

Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA. Electronic address:

Predicting human hepatic clearance remains a fundamental challenge in both pharmaceutical drug development and toxicological assessments of environmental chemicals, with concerns about both accuracy and precision of in vitro-derived estimates. Suggested sources of these issues have included differences in experimental protocols, differences in cell sourcing, and use of a single cell type, liver parenchymal cells (hepatocytes). Here we investigate the ability of human microfluidic four-cell liver acinus microphysiology system (LAMPS) to make predictions as to hepatic clearance for seven representative compounds: Caffeine, Pioglitazone, Rosiglitazone, Terfenadine, Tolcapone, Troglitazone, and Trovafloxacin. Read More

View Article and Full-Text PDF
September 2021

Quantification of NADH:ubiquinone oxidoreductase (complex I) content in biological samples.

J Biol Chem 2021 Sep 17:101204. Epub 2021 Sep 17.

Department of Pediatrics, Division of Neonatology, Columbia University Medical Center, New York, NY 10032, USA. Electronic address:

Impairments in mitochondrial energy metabolism have been implicated in human genetic diseases associated with mitochondrial and nuclear DNA mutations, neurodegenerative and cardiovascular disorders, diabetes, and aging. Alteration in mitochondrial complex I structure and activity has been shown to play a key role in Parkinson's disease and ischemia/reperfusion tissue injury, but significant difficulty remains in assessing the content of this enzyme complex in a given sample. The present study introduces a new method utilizing native polyacrylamide gel electrophoresis in combination with flavin fluorescence scanning to measure the absolute content of complex I, as well as α-ketoglutarate dehydrogenase complex (KGDHC), in any preparation. Read More

View Article and Full-Text PDF
September 2021

A Three-Year Analysis of Ocular Infections in a Tertiary Care University Hospital in Turkey.

Clin Lab 2021 Sep;67(9)

Background: Ocular infections may result in severe vision and eye loss. Especially in keratitis and endophthalmitis, it is essential to identify the causative microorganism and treat it with appropriate antimicrobials. This study aims to investigate microorganisms isolated from various samples in ocular infections and their sensitivity to antibiotics. Read More

View Article and Full-Text PDF
September 2021

Knowledge in Transition in Healthcare.

Eur J Investig Health Psychol Educ 2020 Jul 17;10(3):733-748. Epub 2020 Jul 17.

Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche, Università degli Studi di Pavia, 27100 Pavia, Italy.

Organizations are challenged by the need to transform Dynamic Knowledge, embedded in each worker, into Static Knowledge, rooted in factual documental information. However, innovation and knowledge creation seem to be facilitated by the personal knowledge and life experiences of people, which appear to be dynamic. The tensions between Dynamic and Static Knowledge in facilitating the transfer and sharing of knowledge arise as compelling research as well as practical topic for organizations. Read More

View Article and Full-Text PDF

Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infection.

Physiol Rep 2021 Sep;9(18):e14994

Division of Paediatric Respiratory Medicine and Allergology, Department of Paediatrics, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.

Nasal epithelial cells (NECs) are among the first cells to be exposed to air pollutants and respiratory viruses. Although it is known that air pollution exposure and rhinovirus infections increase the risk for asthma development independently, it is unclear how these risk factors interact on a cellular level. Therefore, we aimed to investigate how exposure to diesel particulate matter (DPM) modifies the response of primary NECs to rhinovirus (RV) infection in vitro. Read More

View Article and Full-Text PDF
September 2021

The impact of poverty and deprivation at the end of life: a critical review.

Palliat Care Soc Pract 2021 12;15:26323524211033873. Epub 2021 Sep 12.

Professor, Te Ārai Palliative Care and End of Life Research Group, School of Nursing, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.

This critical review interrogates what we know about how poverty and deprivation impact people at the end of life and what more we need to uncover. While we know that people in economically resource-rich countries who experience poverty and deprivation over the life course are likely to die younger, with increased co-morbidities, palliative care researchers are beginning to establish a full picture of the disproportionate impact of poverty on how, when and where we die. This is something the Covid-19 pandemic has further illustrated. Read More

View Article and Full-Text PDF
September 2021

Human osteoclast formation and resorptive function on biomineralized collagen.

Bioact Mater 2022 Feb 15;8:241-252. Epub 2021 Jul 15.

Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6229 ER, Maastricht, The Netherlands.

Biomineralized collagen composite materials pose an intriguing alternative to current synthetic bone graft substitutes by offering a biomimetic composition that closely resembles native bone. We hypothesize that this composite can undergo cellular resorption and remodeling similar to natural bone. We investigate the formation and activity of human osteoclasts cultured on biomineralized collagen and pure collagen membranes in comparison to cortical bone slices. Read More

View Article and Full-Text PDF
February 2022

Skin-Lightening Products: Consumer Preferences and Costs.

Cureus 2021 Aug 17;13(8):e17245. Epub 2021 Aug 17.

Dermatology, Boston University School of Medicine, Boston, USA.

Disorders of skin hyperpigmentation have an appreciable impact on quality of life, carrying social and cultural importance that influences widespread consumer demand for cosmetic lightening products. We sought to investigate product factors that may influence consumer preference when choosing an over-the-counter cream for skin-lightening purposes. The keyword "lightening cream" was searched in the "Beauty and Personal Care category" of online retailer Amazon. Read More

View Article and Full-Text PDF

Impact of dietary level and ratio of n-6 and n-3 fatty acids on disease progression and mRNA expression of immune and inflammatory markers in Atlantic salmon () challenged with .

PeerJ 2021 31;9:e12028. Epub 2021 Aug 31.

Institute of Marine Research, Bergen, Norway.

The aim of the study was to investigate the influence of dietary level and ratio of n-6/n-3 fatty acids (FA) on growth, disease progression and expression of immune and inflammatory markers in Atlantic salmon () following challenge with . Fish (80 g) were fed four different diets with different ratios of n-6/n-3 FA; at 1.3, 2. Read More

View Article and Full-Text PDF

Isolation and identification of the bacterium producing antitumor and antimicrobial compounds derived from Iranian swamp frog skin.

Iran J Microbiol 2021 Jun;13(3):372-380

Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Background And Objectives: Cancer incidence and recurrence, antibiotic resistance, and overuse of antibiotics have become a global concern. The purpose of this study was to identify and isolate bacteria from the skin of the , Iranian swamp frog, which has produced antimicrobial compounds, and investigate its cytotoxic activity on the breast (MCF7) and glioblastoma (U87) cancer cell line.

Materials And Methods: An antibiotic-producing bacterium was isolated from the frog skin. Read More

View Article and Full-Text PDF

Study on the value of molecular biology combined with liquid MGIT culture method in clinical examination of mycobacterium tuberculosis.

Jinyou Qi Wei Fan

Am J Transl Res 2021 15;13(8):9757-9763. Epub 2021 Aug 15.

Department of Laboratory Medicine, Huai'an Tumor Hospital Jiangsu, China.

Objective: To investigate the clinical value of molecular biology (xpert MTB/RIF) combined with a liquid mycobacterium culture tube (MGIT) in the clinical examination of Mycobacterium tuberculosis (MTB).

Methods: A total of 782 patients with suspected pulmonary tuberculosis who came to our hospital from February 2018 to February 2020 were selected as the research subjects. Sputum samples of all patients were taken in the morning, and BACTEC MGIT culture and xpert MTB/RIF, Lowenstein-Jenden (L-J) culture and acid fast staining microscopy were used respectively to detect the sputum samples. Read More

View Article and Full-Text PDF

The CD200/CD200R mechanism in mesenchymal stem cells' regulation of dendritic cells.

Am J Transl Res 2021 15;13(8):9607-9613. Epub 2021 Aug 15.

The Second Department of Hematology, Cangzhou Central Hospital 16 West Xinhua Road, Yunhe, Cangzhou, China.

Objective: To investigate the CD200/CD200R pathway mechanism in mesenchymal stem cells' (MSC) regulation of dendritic cells (DC) (MSc).

Methods: We collected marrow samples from 40 patients admitted to our hospital from January 2018 to December 2019. The bone marrow MSCs were cultivated, and the peripheral blood mononuclear cells (PBMC) and peripheral blood DC were isolated to establish an in vitro immune response model. Read More

View Article and Full-Text PDF

Reduced Risk of Atrial Fibrillation Following Cholecystectomy: A Nationwide Population-Based Study.

Front Aging Neurosci 2021 2;13:706815. Epub 2021 Sep 2.

Graduate Institute of Clinical Medicine Science and School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan.

: Gallstone disease (GD) is associated with a high risk of cardiovascular disease. However, it is unknown whether GD contributes to atrial fibrillation (AF). We aimed to investigate the association between GD and AF. Read More

View Article and Full-Text PDF
September 2021

Thymus Extracellular Matrix-Derived Scaffolds Support Graft-Resident Thymopoiesis and Long-Term In Vitro Culture of Adult Thymic Epithelial Cells.

Adv Funct Mater 2021 May 12;31(20):2010747. Epub 2021 Mar 12.

Department of Biomedicine University Hospital Basel University of Basel Basel 4031 Switzerland.

The thymus provides the physiological microenvironment critical for the development of T lymphocytes, the cells that orchestrate the adaptive immune system to generate an antigen-specific response. A diverse population of stroma cells provides surface-bound and soluble molecules that orchestrate the intrathymic maturation and selection of developing T cells. Forming an intricate 3D architecture, thymic epithelial cells (TEC) represent the most abundant and important constituent of the thymic stroma. Read More

View Article and Full-Text PDF

Between Handholding and Hand-held Devices: Marketing Through Smartphone Innovation and Women's Entrepreneurship in Post Conflict Economies in Times of Crisis.

Inf Syst Front 2021 Sep 15:1-23. Epub 2021 Sep 15.

Excelia Business School, CERIIM, La Rochelle, France.

There is a wide gender gap in developing countries due to a range of cultural, economic and political barriers. This is even more accentuated in post conflict economies in times of crisis. Smartphones and mobile applications can help to narrow this gap. Read More

View Article and Full-Text PDF
September 2021


Wiad Lek 2021 ;74(8):1839-1843


Objective: The aim: Was to investigate human-beta-defensin-1 level in blood serum depending on tuberculosis severity and treatment ef f ectiveness.

Patients And Methods: Materials and methods: 100 patients with pulmonary tuberculosis and 20 healthy persons were included to the study. HBD-1 level was measured by ELISA in all the healthy persons and in all the patients at the treatment onset and at the end of initial phase of treatment. Read More

View Article and Full-Text PDF
September 2021

Applications of Brain Organoids for Infectious Diseases.

J Mol Biol 2021 Sep 15:167243. Epub 2021 Sep 15.

Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA19104, USA; Department of Developmental and Cell Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA19104, USA; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA19104, USA. Electronic address:

Brain organoids are self-organized three-dimensional aggregates generated from pluripotent stem cells. They exhibit complex cell diversities and organized architectures that resemble human brain development ranging from neural tube formation, neuroepithelium differentiation, neurogenesis and gliogenesis, to neural circuit formation. Rapid advancements in brain organoid culture technologies have allowed researchers to generate more accurate models of human brain development and neurological diseases. Read More

View Article and Full-Text PDF
September 2021

Current status of group B infection in neonates: a multicenter prospective study.

Zhongguo Dang Dai Er Ke Za Zhi 2021 Sept 15;23(9):889-895

Department of Neonatology, Women and Children's Hospital, School of Medcine, Xiamen University/Xiamen Maternal and Child Care Hospital/Xiamen Key Laboratory of Perinatal-Neonatal Infection, Xiamen, Fujian 361003, China (Lin X-Z, Email: [email protected] 163. com).

Objectives: To investigate the incidence of maternal group B (GBS) colonization and neonatal early-onset GBS disease (GBS-EOD), and to study the factors associated with the development of GBS-EOD in the offspring of pregnant women with GBS colonization.

Methods: A total of 16 384 pregnant women and 16 634 neonates delivered by them were enrolled prospectively who had medical records in Xiamen Maternal and Child Care Hospital, Beijing Obstetrics and Gynecology Hospital of Capital Medical University, and Zhangzhou Zhengxing Hospital from May 1, 2019 to April 30, 2020. Unified GBS screening time, culture method, and indication for intrapartum antibiotic prophylaxis (IAP) were adopted in the three hospitals. Read More

View Article and Full-Text PDF
September 2021

Microrna analysis of human decidua mesenchymal stromal cells from preeclampsia patients.

Placenta 2021 Sep 10;115:12-19. Epub 2021 Sep 10.

Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul, Turkey. Electronic address:

Introduction: In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed to abnormally high levels of oxidative stress and inflammatory factors circulating in the maternal blood. MicroRNAs (miRNAs) have been shown to have a significant impact on the differentiation, maturation and function of mesenchymal stromal cells (MSCs). Our aim in the present study is firstly to investigate differentially expressed miRNA levels to be used as a biomarker in the early detection of PE and secondly to investigate whether those differentially expressed miRNAs in hDMSCs have an effect on the pathogenesis of PE. Read More

View Article and Full-Text PDF
September 2021

Kidney toxicity of the BRAF-kinase inhibitor vemurafenib is driven by off-target ferrochelatase inhibition.

Kidney Int 2021 Sep 14. Epub 2021 Sep 14.

Division of Pharmaceutics and Pharmacology, College of Pharmacy & Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA. Electronic address:

A multitude of disease and therapy related factors drive the frequent development of kidney disorders in cancer patients. Along with chemotherapy, the newer targeted therapeutics can also cause kidney dysfunction through on and off-target mechanisms. Interestingly, among the small molecule inhibitors approved for the treatment of cancers that harbor BRAF-kinase activating mutations, vemurafenib can trigger tubular damage and acute kidney injury. Read More

View Article and Full-Text PDF
September 2021