204 results match your criteria intravesical capsaicin

Effects of intravesical prostaglandin E on bladder function are preserved in capsaicin-desensitized rats.

Am J Physiol Renal Physiol 2021 02 7;320(2):F212-F223. Epub 2020 Dec 7.

Department of Biomedical Engineering, Duke University, Durham, North Carolina.

Prostaglandin E (PGE) instilled into the bladder generates symptoms of urinary urgency in healthy women and reduces bladder capacity and urethral pressure in both humans and female rats. Systemic capsaicin desensitization, which causes degeneration of C-fibers, prevented PGE-mediated reductions in bladder capacity, suggesting that PGE acts as an irritant (Maggi CA, Giuliani S, Conte B, Furio M, Santicioli P, Meli P, Gragnani L, Meli A. 145: 105-112, 1988). Read More

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February 2021

Responsiveness of lumbosacral superficial dorsal horn neurons during the voiding reflex and functional loss of spinal urethral-responsive neurons in streptozotocin-induced diabetic rats.

Neurourol Urodyn 2020 01 29;39(1):144-157. Epub 2019 Oct 29.

Department of Neurophysiology, Hyogo College of Medicine, Nishinomiya, Japan.

Aims: Sensory information from the lower urinary tract (LUT) is conveyed to the spinal cord to trigger and co-ordinate micturition. However, it is not fully understood how spinal dorsal horn neurons are excited during the voiding reflex. In this study, we developed an in vivo technique allowing recording of superficial dorsal horn (SDH) neurons concurrent with intravesical pressure (IVP) during the micturition cycle in both normal and diabetic rats. Read More

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January 2020

Effects of intravesical administration of sensory neuron-specific receptor agonist on voiding function in rats with cyclophosphamide-induced cystitis.

Cent European J Urol 2019 6;72(2):142-148. Epub 2019 May 6.

Tottori University Faculty of Medicine, Department of Urology, Yonago, Japan.

Introduction: The aim of this study was to investigate the urodynamic effects of intravesical administration of bovine adrenal medulla 8-22 (BAM8-22), a selective rat sensory neuron-specific receptor 1 agonist, on the micturition reflex in normal rats and rats with cyclophosphamide-induced bladder overactivity.

Material And Methods: Continuous cystometrograms (0.04 ml/min) were performed in urethane-anesthetized rats. Read More

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Neonatal Bladder Irritation Is Associated With Vanilloid Receptor TRPV1 Expression in Adult Rats.

Int Neurourol J 2018 Sep 28;22(3):169-176. Epub 2018 Sep 28.

Department of Urology, Yonsei University College of Medicine, Seoul, Korea.

Purpose: To evaluate whether mild chemical irritation of the bladder in neonatal rats is associated with persistent vanilloid receptor transient receptor potential vanilloid subfamily 1 (TRPV1) activity in adult rats.

Methods: Female Sprague-Dawley rats were used. Ten-day-old rat pups underwent bladder sensitization via intravesical infusion of 0. Read More

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September 2018

Reduced bladder responses to capsaicin and GSK-1016790A in retired-breeder female rats with diminished volume sensitivity.

Am J Physiol Renal Physiol 2018 11 18;315(5):F1217-F1227. Epub 2018 Jul 18.

Department of Urology, the Second Hospital of Shandong University, Jinan, Shandong, Peoples Republic of China.

Literature documents an age-related reduction of bladder sensory function. Transient receptor potential vanilloid (TRPV)1 or TRPV4 channels have been implicated in bladder mechanotransduction. To investigate contributions of TRPV1 or TRPV4 to the age-related reduction of bladder sensory function, bladder responses to capsaicin (CAP; TRPV1 agonist) and GSK-1016790A (GSK; TRPV4 agonist) in retired breeder (RB; 12-15 mo) and young adult (2-3 mo) female rats were compared using multiple methods. Read More

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November 2018

TRPV1 enhances the afferent response to P2X receptor activation in the mouse urinary bladder.

Sci Rep 2018 01 9;8(1):197. Epub 2018 Jan 9.

Centre for Urology Research, Faculty of Health Science and Medicine, Bond University, Gold Coast, Queensland, 4229, Australia.

Both TRPV1 and P2X receptors present on bladder sensory nerve fibres have been implicated in mechanosensation during bladder filling. The aim of this study was to determine possible interactions between these receptors in modulating afferent nerve activity. In wildtype (TRPV1) and TRPV1 knockout (TRPV1) mice, bladder afferent nerve activity, intravesical pressure, and luminal ATP and acetylcholine levels were determined and also intracellular calcium responses of dissociated pelvic DRG neurones and primary mouse urothelial cells (PMUCs). Read More

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January 2018

Intravesical vanilloids for treating neurogenic lower urinary tract dysfunction in patients with multiple sclerosis: A systematic review and meta-analysis. A report from the Neuro-Urology Promotion Committee of the International Continence Society (ICS).

Neurourol Urodyn 2018 01 15;37(1):67-82. Epub 2017 Jun 15.

Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, Zürich, Switzerland.

Aims: To systematically assess all available evidence on efficacy and safety of vanilloids for treating neurogenic lower urinary tract dysfunction (NLUTD) in patients with multiple sclerosis (MS).

Methods: This systematic review and meta-analysis was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Studies were identified by electronic search of Cochrane register, Embase, Medline, Scopus, (last search January 8, 2016). Read More

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January 2018

Manipulating the extracellular matrix: an animal model of the bladder pain syndrome.

Pain 2017 01;158(1):161-170

Neurorestoration Group, Wolfson CARD, King's College London, London, United Kingdom.

Bladder pain syndrome (BPS) is associated with breakdown of the protective uroepithelial barrier of the urinary bladder allowing urinary constituents access to bladder sensory neurons. Although there are several animal models of cystitis, none specifically relates to BPS. Here, we aimed to create such a model using enzymatic digestion of the barrier proteoglycans (PGs) in the rat. Read More

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January 2017

Decreased inflammatory response in rat bladder after intravesical administration of capsaicin-loaded liposomes.

An Acad Bras Cienc 2016 Sep 1;88(3):1539-47. Epub 2016 Sep 1.

Programa de Pós-Graduação em Saúde e Ambiente e Biotecnologia Industrial, Curso de Farmácia, Universidade Tiradentes, Av. Murilo Dantas, 300, Farolândia, 49032-490 Aracaju, SE, Brasil.

The objective of this work was to study the reduction in the capsaicin toxicity by encapsulation in liposomes. Capsaicin was extracted from peppers and characterized with high performance liquid chromatography (HPLC). We determined the zeta potential, polydispersivity index (PdI) and vesicle size of liposomes. Read More

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September 2016

Capsaicin triggers autophagic cell survival which drives epithelial mesenchymal transition and chemoresistance in bladder cancer cells in an Hedgehog-dependent manner.

Oncotarget 2016 Aug;7(31):50180-50194

School of Pharmacy, Experimental Medicine Section, University of Camerino, Camerino, Italy.

Bladder cancer (BC) is a common urologic tumor characterized by high risk of recurrence and mortality. Capsaicin (CPS), used as an intravesical drug for overactive bladder, was demonstrated to induce cell death in different cancer cells including BC cells.Here we found that treatment of high-grade BC cells with high dose of CPS triggers autophagy. Read More

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Botulinum toxin A for the Treatment of Overactive Bladder.

Toxins (Basel) 2016 Feb 29;8(3). Epub 2016 Feb 29.

Department of Urology, China Medical University Hospital, Taichung 40447, Taiwan.

The standard treatment for overactive bladder starts with patient education and behavior therapies, followed by antimuscarinic agents. For patients with urgency urinary incontinence refractory to antimuscarinic therapy, currently both American Urological Association (AUA) and European Association of Urology (EAU) guidelines suggested that intravesical injection of botulinum toxin A should be offered. The mechanism of botulinum toxin A includes inhibition of vesicular release of neurotransmitters and the axonal expression of capsaicin and purinergic receptors in the suburothelium, as well as attenuation of central sensitization. Read More

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February 2016

Social stress in mice induces urinary bladder overactivity and increases TRPV1 channel-dependent afferent nerve activity.

Am J Physiol Regul Integr Comp Physiol 2015 Sep 29;309(6):R629-38. Epub 2015 Jul 29.

Department of Pharmacology, University of Vermont College of Medicine, Burlington, Vermont; Institute of Cardiovascular Sciences, University of Manchester, Manchester, United Kingdom.

Social stress has been implicated as a cause of urinary bladder hypertrophy and dysfunction in humans. Using a murine model of social stress, we and others have shown that social stress leads to bladder overactivity. Here, we show that social stress leads to bladder overactivity, increased bladder compliance, and increased afferent nerve activity. Read More

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September 2015

Pelvic organ cross-sensitization to enhance bladder and urethral pain behaviors in rats with experimental colitis.

Neuroscience 2015 Jan 15;284:422-429. Epub 2014 Oct 15.

Department of Urology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address:

Neural cross-sensitization has been postulated as a mechanism underlying overlaps of chronic pelvic pain disorders such as bladder pain syndrome/interstitial cystitis (BPS/IC) and irritable bowel syndrome (IBS). Animals with experimental colitis have been used to study the underlying mechanisms for overlapped pelvic pain symptoms, and shown to exhibit bladder overactivity evidenced by frequent voiding; however, it has not directly been evaluated whether pain sensation derived from the lower urinary tract is enhanced in colitis models. Also, the cross-sensitization between the colon and urethra has not been studied previously. Read More

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January 2015

Hydrogen sulfide plays a key role in the inhibitory neurotransmission to the pig intravesical ureter.

PLoS One 2014 21;9(11):e113580. Epub 2014 Nov 21.

Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.

According to previous observations nitric oxide (NO), as well as an unknown nature mediator are involved in the inhibitory neurotransmission to the intravesical ureter. This study investigates the hydrogen sulfide (H2S) role in the neurogenic relaxation of the pig intravesical ureter. We have performed western blot and immunohistochemistry to study the expression of the H2S synthesis enzymes cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), measurement of enzymatic production of H2S and myographic studies for isometric force recording. Read More

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December 2015

Selective inhibitory effect of imidafenacin and 5-hydroxymethyl tolterodine on capsaicin sensitive C fibers of the primary bladder mechanosensitive afferent nerves in the rat.

J Urol 2015 Apr 16;193(4):1423-32. Epub 2014 Sep 16.

Departments of Continence Medicine and Urology (TF, MS, HF, YH), University of Tokyo Graduate School of Medicine, Tokyo, Japan. Electronic address:

Purpose: Imidafenacin and fesoterodine are used to treat overactive bladder. Imidafenacin, fesoterodine and its active metabolite 5-hydroxymethyl tolterodine are muscarinic receptor antagonists. It is believed that these agents act on afferent nerves in addition to smooth muscle. Read More

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Use of vanilloids in urologic disorders.

Prog Drug Res 2014 ;68:307-17

The bladder is an organ rich in vanilloid targets: dense unmyelinated c-fibers partially responsible for bladder sensation and response to noxious stimuli. Drugs such as capsaicin and resiniferatoxin (RTX) interact with the VR1 vanilloid receptor subtype to initially excite then subsequently desensitize the c-fibers. This chapter examines the literature describing the use of vanilloid receptor agonists in the treatment of the following urological disorders: neurogenic bladder (NGB), overactive bladder (OAB), and interstitial cystitis/painful bladder syndrome (IC/PBS). Read More

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TRP channels in lower urinary tract dysfunction.

Br J Pharmacol 2014 May;171(10):2537-51

Lower urinary tract dysfunction (LUTd) represents a major healthcare problem. Although it is mostly not lethal, associated social disturbance, medical costs, loss of productivity and especially diminished quality of life should not be underestimated. Although more than 15% of people suffer from a form of LUTd to some extent, pathophysiology often remains obscure. Read More

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Sensory dysfunction of bladder mucosa and bladder oversensitivity in a rat model of metabolic syndrome.

PLoS One 2012 19;7(9):e45578. Epub 2012 Sep 19.

Division of Urology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Purpose: To study the role of sensory dysfunction of bladder mucosa in bladder oversensitivity of rats with metabolic syndrome.

Materials And Methods: Female Wistar rats were fed a fructose-rich diet (60%) or a normal diet for 3 months. Based on cystometry, the fructose-fed rats (FFRs) were divided into a group with normal detrusor function or detrusor overactivity (DO). Read More

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JTS-653 blocks afferent nerve firing and attenuates bladder overactivity without affecting normal voiding function.

J Urol 2013 Mar 8;189(3):1137-46. Epub 2012 Oct 8.

Central Pharmaceutical Research Institute, Japan Tobacco, Inc., Osaka, Japan.

Purpose: We evaluated the role of TRPV1 in bladder overactivity based on afferent nerve firing and urodynamic parameters using the selective TRPV1 antagonist JTS-653.

Materials And Methods: We evaluated the effects of JTS-653 on the increased pelvic nerve discharge and intravesical pressure induced by intravesical infusion of 100 μM capsaicin in anesthetized rats. The effects of JTS-653 on the urodynamic parameters of bladder overactivity induced by intravesical infusion of 30 nM resiniferatoxin or 0. Read More

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The use of cystometry in small rodents: a study of bladder chemosensation.

J Vis Exp 2012 Aug 21(66):e3869. Epub 2012 Aug 21.

Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven, Belgium.

The lower urinary tract (LUT) functions as a dynamic reservoir that is able to store urine and to efficiently expel it at a convenient time. While storing urine, however, the bladder is exposed for prolonged periods to waste products. By acting as a tight barrier, the epithelial lining of the LUT, the urothelium, avoids re-absorption of harmful substances. Read More

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[Standard pharmacological treatment and new therapies for overactive bladder].

Urologia 2012 ;79(1):6-13

Neuro-Urologia, Azienda Ospedaliero-Universitaria Careggi, Firenze, Italy.

The prevalence of overactive bladder (OAB) in adult males varies from 10.2% to 17.4%, and in females from 7. Read More

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Effects of TRPV4 cation channel activation on the primary bladder afferent activities of the rat.

Neurourol Urodyn 2012 Jan 28;31(1):148-55. Epub 2011 Oct 28.

Department of Continence Medicine, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.

Aims: Transient receptor potential vanilloid 4 (TRPV4) may affect afferent pathways innervating the bladder. We investigated the effects of GSK1016790A (GSK) and RN1734, a TRPV4 agonist and antagonist, respectively, and P2X-purinoceptor antagonists (TNP-ATP and PPADS) on cystometry (CMG), and the effect of GSK on single afferent fiber activities (SAAs) of the rat bladder and its relationship with capsaicin (Cap)-sensitivity.

Methods: Conscious female Sprague-Dawley rats were used for CMG measurements. Read More

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January 2012

Effect of partial and complete blockade of vanilloid (TRPV1-6) and ankyrin (TRPA1) transient receptor potential ion channels on urinary bladder motor activity in an experimental hyperosmolar overactive bladder rat model.

J Physiol Pharmacol 2011 Jun;62(3):321-6

Department of Pathophysiology, Jagiellonian University, Medical College, Cracow, Poland.

Unlabelled: The study investigated the mechanisms through which the hyperosmolarity might induce detrusor overactivity (DO). We compared the bladder activity in response to partial and complete blockade of TRPV1-6 and TRPA1 receptors. Experiments were performed on 42 rats. Read More

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Sensory neurone-specific receptor-mediated regulation of micturition reflex in urethane-anaesthetized rats.

BJU Int 2012 Feb 5;109(4):628-33. Epub 2011 Jul 5.

Department of Urology, Tottori University School of Medicine, Yonago, Japan.

Objective: •  To investigate the effect of sensory neurone-specific receptors (SNSRs) activation on the micturition reflex in rats.

Materials And Methods: •  Continuous cystometrograms (CMGs, 0.04 mL/min) were performed in female Sprague-Dawley rats under urethane anaesthesia. Read More

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February 2012

Therapeutic targeting of TRPV1 by resiniferatoxin, from preclinical studies to clinical trials.

Curr Top Med Chem 2011 ;11(17):2159-70

Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

In primary sensory neurons, the capsaicin receptor TRPV1 functions as a molecular integrator for a broad range of seemingly unrelated chemical and physical noxious stimuli, including heat and altered pH. Indeed, TRPV1 is thought to be a major transducer of the thermal hyperalgesia that follows inflammation and tissue injury as this response is impaired in TRPV1-deficient mice. Following the molecular cloning of TRPV1 in 1997, over a dozen companies embarked on efforts to find clinically useful TRPV1 antagonists, but side-effects and limited efficacy have thus far prevented any compounds from progressing beyond phase II. Read More

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An exploration of the control of micturition using a novel in situ arterially perfused rat preparation.

Front Neurosci 2011 13;5:62. Epub 2011 May 13.

School of Physiology and Pharmacology, University of Bristol Bristol, UK.

Our goal was to develop and refine a decerebrate arterially perfused rat (DAPR) preparation that allows the complete bladder filling and voiding cycle to be investigated without some of the restrictions inherent with in vivo experimentation [e.g., ease and speed of set up (30 min), control over the extracellular milieu and free of anesthetic agents]. Read More

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Are antimuscarinic drugs effective against urinary frequency mediated by atropine-resistant contractions?

J Pharmacol Sci 2011 22;115(3):364-73. Epub 2011 Feb 22.

Department of Applied Pharmacology, Tokushima Research Center, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.

In the disease states of urinary frequency and urgency, atropine-resistant contractions are known to be involved, in addition to contractions mediated by cholinergic nerves. This study was undertaken to investigate the mechanism underlying the development of atropine-resistant contractions using the representative antimuscarinic drugs solifenacin and tolterodine and also propiverine that has Ca(2+) channel-antagonizing activity in addition to antimuscarinic activity. Rat models of urinary frequency were established by intravesical infusion of acetylcholine (ACh) (cholinergic nerve-mediated urinary frequency model), acetic acid (AcOH) [non-adrenergic non-cholinergic nerve (NANC)-mediated urinary frequency model], or CaCl(2) (atropine-resistant contractions-mediated urinary frequency model). Read More

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October 2011

[Capsaicin and lidocaine usage in functional disorders of urinary bladder].

Folia Med Cracov 2011 ;51(1-4):91-8

Katedra Patofizjologii, Uniwersytet Jagielloński Collegium Medicum, Kraków.

Most of the drugs in the treatment of functional disorders of the urinary bladder has a peripheral effect. Their work consists mainly in reducing detrusor contractility of the bladder, or effects on the afferent innervation. Anticholinergics are the first drugs of choice. Read More

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September 2012

Effects of stimulation of muscarinic receptors on bladder afferent nerves in the in vitro bladder-pelvic afferent nerve preparation of the rat.

Brain Res 2010 Nov 16;1361:43-53. Epub 2010 Sep 16.

Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Effects of a muscarinic receptor agonist oxotremorine-M (oxo-M) on bladder afferent nerve (BAN) activity were studied in an in vitro bladder-pelvic nerve preparation. Distension of the bladder induced rhythmic bladder contractions that were accompanied by multiunit afferent firing. Intravesical administration of 25 and 50 μM oxo-M significantly increased afferent firing from 41 ± 2 spikes/s to 51 ± 4 spikes/s and 60. Read More

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November 2010

Management of Low Compliant Bladder in Spinal Cord Injured Patients.

Won Hee Park

Low Urin Tract Symptoms 2010 Sep 30;2(2):61-70. Epub 2010 Jul 30.

Department of Urology, Inha University College of Medicine, Incheon, Korea.

Low bladder compliance means an abnormal volume and pressure relationship, and an incremental rise in bladder pressure during the bladder filling. It is well known that at the time bladder capacity decreases, intravesical pressure increases, and the risk of upper deterioration increases. Hypocompliance is usually thought to be the range from 1. Read More

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September 2010