6,585 results match your criteria interactions pseudomonas


Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.

Environ Res 2021 May 11:111321. Epub 2021 May 11.

Department of Biotechnology, Modern College of Arts, Science and Commerce (Savitribai Phule Pune University), Ganeshkhind, Pune, 411016, India; Department of Environmental Science, Savitribai Phule Pune University, Pune, 411007, India. Electronic address:

A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health. Unfortunately, ABR is no longer confined to nosocomial environments and is frequently reported from community microbes as well. The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics. Read More

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Denitrifying bacteria respond to and shape microscale gradients within particulate matrices.

Commun Biol 2021 May 13;4(1):570. Epub 2021 May 13.

Department of Earth, Atmospheric & Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.

Heterotrophic denitrification enables facultative anaerobes to continue growing even when limited by oxygen (O) availability. Particles in particular provide physical matrices characterized by reduced O permeability even in well-oxygenated bulk conditions, creating microenvironments where microbial denitrifiers may proliferate. Whereas numerical particle models generally describe denitrification as a function of radius, here we provide evidence for heterogeneity of intraparticle denitrification activity due to local interactions within and among microcolonies. Read More

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The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling.

Cell 2021 May 11. Epub 2021 May 11.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane. Read More

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The TIR-NBS protein TN13 associates with the CC-NBS-LRR resistance protein RPS5 and contributes to RPS5-triggered immunity in Arabidopsis.

Plant J 2021 May 13. Epub 2021 May 13.

State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Nucleotide binding-leucine rich repeat (NLR) proteins play important roles in plant innate immunity by recognizing pathogen effectors. The TIR-NBS (TN) proteins belong to a subtype of the atypical NLRs, but their function in plant immunity is poorly understood. The well-characterized Arabidopsis thaliana (Arabidopsis) typical CC-NBS-LRR (CNL) protein RESISTANCE TO PSEUDOMONAS SYRINGAE 5 (RPS5) is activated after recognizing the Pseudomonas syringae type III effector AvrPphB. Read More

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PaIntDB: Network-based omics integration and visualization using protein-protein interactions in Pseudomonas aeruginosa.

Bioinformatics 2021 May 12. Epub 2021 May 12.

Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada.

Summary: The Pseudomonas aeruginosa Interaction Database, PaIntDB, is an intuitive web-based tool for network-based systems biology analyses using protein-protein interactions (PPI) in this medically-important pathogen. It enables the integration and visualization of omics analyses including RNA-Seq and Tn-Seq. High-throughput datasets can be mapped onto PPI networks, which can be explored visually and filtered to uncover novel putative molecular pathways related to the conditions of study. Read More

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A Metabolite of Triggers Prophage-Selective Lysogenic to Lytic Conversion in .

J Am Chem Soc 2021 May 12. Epub 2021 May 12.

Department of Chemistry, Konstanz Research School Chemical Biology, Zukunftskolleg, University of Konstanz, 78457 Konstanz, Germany.

Bacteriophages have major impact on their microbial hosts and shape entire microbial communities. The majority of these phages are latent and reside as prophages integrated in the genomes of their microbial hosts. A variety of intricate regulatory systems determine the switch from a lysogenic to lytic life style, but so far strategies are lacking to selectively control prophage induction by small molecules. Read More

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Neutralizing antibody response against subcutaneously injected bacteriophages in rabbit model.

Virusdisease 2021 Mar 10;32(1):38-45. Epub 2021 Mar 10.

Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005 India.

Bacteriophage therapy is currently experiencing a renaissance. Therapeutic efficacy of bacteriophages depends on phage-bacterial and phage-host interactions. The appearance of neutralizing anti-phage antibody has been speculated to be one of the few reasons for bacteriophage therapy's failure. Read More

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Albumin-Based LL37 Peptide Nanoparticles as a Sustained Release System against Lung Infection.

ACS Biomater Sci Eng 2021 May 31;7(5):1817-1826. Epub 2020 Aug 31.

Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China.

(PA) has emerged as a pressing challenge to pulmonary infection and lung damage. The LL37 peptide is an efficient antimicrobial agent against PA strains, but its application is limited because of fast clearance , biosafety concerns, and low bioavailability. Thus, an albumin-based nanodrug delivery system with reduction sensitivity was developed by forming intermolecular disulfide bonds to increase LL37 performance against PA. Read More

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Geochemical, metagenomic, and physiological characterization of the multifaceted interaction between microbiome of an arsenic contaminated groundwater and aquifer sediment.

J Hazard Mater 2021 Jun 11;412:125099. Epub 2021 Jan 11.

Environmental Microbiology and Genomics Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India. Electronic address:

Geomicrobiological details of the interactions between groundwater microbiome (GWM) and arsenic (As)-rich aquifer sediment of Bengal basin was investigated through microcosm incubations. Role of key microorganisms and their specific interactions with As-bearing minerals was demarcated under organic carbon- amended and -unamended conditions. Acinetobacter (50. Read More

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High-Throughput Approaches for the Identification of Antivirulents.

mBio 2021 05 4;12(3). Epub 2021 May 4.

Department of Biosciences, Rice University, Houston, Texas, USA

Antimicrobial resistance is a serious medical threat, particularly given the decreasing rate of discovery of new treatments. Although attempts to find new treatments continue, it has become clear that merely discovering new antimicrobials, even if they are new classes, will be insufficient. It is essential that new strategies be aggressively pursued. Read More

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Distinctive features of the Gac-Rsm pathway in plant-associated Pseudomonas.

Environ Microbiol 2021 May 3. Epub 2021 May 3.

Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ-CSIC), Granada, Spain.

Productive plant-bacteria interactions, either beneficial or pathogenic, require that bacteria successfully sense, integrate and respond to continuously changing environmental and plant stimuli. They use complex signal transduction systems that control a vast array of genes and functions. The Gac-Rsm global regulatory pathway plays a key role in controlling fundamental aspects of the apparently different lifestyles of plant beneficial and phytopathogenic Pseudomonas as it coordinates adaptation and survival while either promoting plant health (biocontrol strains) or causing disease (pathogenic strains). Read More

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Root Exudates Alter the Expression of Diverse Metabolic, Transport, Regulatory, and Stress Response Genes in Rhizosphere .

Front Microbiol 2021 14;12:651282. Epub 2021 Apr 14.

School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, United States.

Plants live in association with microorganisms that positively influence plant development, vigor, and fitness in response to pathogens and abiotic stressors. The bulk of the plant microbiome is concentrated belowground at the plant root-soil interface. Plant roots secrete carbon-rich rhizodeposits containing primary and secondary low molecular weight metabolites, lysates, and mucilages. Read More

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A γ-lactam siderophore antibiotic effective against multidrug-resistant Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter spp.

Eur J Med Chem 2021 Apr 8;220:113436. Epub 2021 Apr 8.

Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA; Department of Biochemistry, Case Western Reserve University, Cleveland, OH, 44106, USA; Department of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA; Geriatric Research, Education and Clinical Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, 44106, USA; Departments of Pharmacology, Molecular Biology & Microbiology, And Proteomics & Bioinformatics, Case Western Reserve University, Cleveland, OH, 44106, USA; CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, OH, 44106, USA. Electronic address:

Serious infections caused by multidrug-resistant (MDR) organisms (Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii) present a critical need for innovative drug development. Herein, we describe the preclinical evaluation of YU253911, 2, a novel γ-lactam siderophore antibiotic with potent antimicrobial activity against MDR Gram-negative pathogens. Penicillin-binding protein (PBP) 3 was shown to be a target of 2 using a binding assay with purified P. Read More

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Streptomyces strains modulate dynamics of soil bacterial communities and their efficacy in disease suppression caused by Phytophthora capsici.

Sci Rep 2021 Apr 29;11(1):9317. Epub 2021 Apr 29.

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

The responses of rhizosphere bacterial communities of Streptomyces (SS14 and IT20 stains) treated-pepper plants following inoculation by Phytophthora capsici (PC) was investigated using Illumina MiSeq sequencing. Distinct modulation of the bacteriome composition was found for PC samples with the highest relative abundance (RA) of Chitinophaga (22 ± 0.03%). Read More

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Mayaro Virus: The Potential Role of Microbiota and .

Pathogens 2021 Apr 27;10(5). Epub 2021 Apr 27.

Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Instituto René Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil.

The Mayaro virus (MAYV) is an arbovirus that circulates mainly in tropical forests or rural areas in Latin America and is transmitted mainly by mosquitoes. The objective of this study was to evaluate the vector competence, microbiome, and the presence of in three populations infected with MAYV. The vector competence was assessed based on viral infection and transmission by RT-qPCR. Read More

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Molecular Modifications of the Pseudomonas Quinolone Signal in the Intermicrobial Competition with .

J Fungi (Basel) 2021 Apr 28;7(5). Epub 2021 Apr 28.

Infectious Diseases Research Laboratory, California Institute for Medical Research, San Jose, CA 95128, USA.

The Pseudomonas quinolone signal (PQS) is an important quorum-sensing molecule for that regulates virulence factors, chelates iron, and is an important factor in interactions with eukaryotes, including fungi and mammalian hosts. It was previously shown to inhibit or boost , depending on the milieu iron concentration. We studied several molecular modifications of the PQS molecule, and their effects on biofilm metabolism and growth in vitro, and the effects of iron supplementation. Read More

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Expiration Date of Ready-to-Eat Salads: Effects on Microbial Load and Biochemical Attributes.

Foods 2021 Apr 25;10(5). Epub 2021 Apr 25.

Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3036 Limassol, Cyprus.

When minimally processed vegetables reach their expiration date, expose an increased microbial load. This includes mainly spoilage microorganisms but also foodborne pathogens, thus affecting the quality and safety of highly consumed ready-to-eat salads. A total of 144 ready-to-eat salads from the Cypriot market were analyzed in an attempt to determine the effects of the expiration date on the microbial load and plant metabolic variables of the salads. Read More

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and : Emerging Pathogens Well-Armed for Life in the Cystic Fibrosis Patients' Lung.

Genes (Basel) 2021 Apr 21;12(5). Epub 2021 Apr 21.

HydroSciences Montpellier, CNRS, IRD, Univ Montpellier, Service de Microbiologie et Hygiène Hospitalière, CHU Nîmes, 34093 Nîmes, France.

In patients with cystic fibrosis (CF), the lung is a remarkable ecological niche in which the microbiome is subjected to important selective pressures. An inexorable colonization by bacteria of both endogenous and environmental origin is observed in most patients, leading to a vicious cycle of infection-inflammation. In this context, long-term colonization together with competitive interactions among bacteria can lead to over-inflammation. Read More

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Milieu matters: An wound milieu to recapitulate key features of, and probe new insights into, mixed-species bacterial biofilms.

Biofilm 2021 Dec 3;3:100047. Epub 2021 Apr 3.

Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India.

Bacterial biofilms are a major cause of delayed wound healing. Consequently, the study of wound biofilms, particularly in host-relevant conditions, has gained importance. Most studies employ refined laboratory media to study biofilms, representing conditions that are not relevant to the infection state. Read More

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December 2021

Genomic insights into the molecular mechanisms of a Pseudomonas strain significant in its survival in Kongsfjorden, an Arctic fjord.

Mol Genet Genomics 2021 Apr 28. Epub 2021 Apr 28.

National Centre for Polar and Ocean Research, Ministry of Earth Sciences, Headland Sada, Vasco da Gama, 403804, Goa, India.

Whole-genome sequence of Pseudomonas sp. Kongs-67 retrieved from Kongsfjorden, an Arctic fjord, has been investigated to understand the molecular machinery required for microbial association and survival in a polar fjord. The genome size of Kongs-67 was 4. Read More

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Collective colony growth is optimized by branching pattern formation in Pseudomonas aeruginosa.

Mol Syst Biol 2021 04;17(4):e10089

Department of Biomedical Engineering, Duke University, Durham, NC, USA.

Branching pattern formation is common in many microbes. Extensive studies have focused on addressing how such patterns emerge from local cell-cell and cell-environment interactions. However, little is known about whether and to what extent these patterns play a physiological role. Read More

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The intracellular phase of extracellular respiratory tract bacterial pathogens and its role on pathogen-host interactions during infection.

Curr Opin Infect Dis 2021 Jun;34(3):197-205

Interfaculty Institute for Genetics and Functional Genomics, Department of Functional Genomics, University Medicine Greifswald, Greifswald, Germany.

Purpose Of Review: An initial intracellular phase of usually extracellular bacterial pathogens displays an important strategy to hide from the host's immune system and antibiotics therapy. It helps the bacteria, including bacterial pathogens of airway diseases, to persist and eventually switch to a typical extracellular infection. Several infectious diseases of the lung are life-threatening and their control is impeded by intracellular persistence of pathogens. Read More

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Chorismate mutase peptide antibody enables specific detection of Acanthamoeba.

PLoS One 2021 23;16(4):e0250342. Epub 2021 Apr 23.

Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Korea.

Accurate and rapid diagnosis of Acanthamoeba keratitis (AK) is difficult. Although the diagnostic procedure for AK has improved, further development and effective diagnostic tool utilization for AK need to continue. Chorismate mutase is a key regulatory enzyme involved in the shikimate pathway, a metabolic pathway absent in mammals but central for amino acid biosynthesis in bacteria, fungi, algae, and plants. Read More

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The vesicular trafficking system component MIN7 is required for minimizing Fusarium graminearum infection.

J Exp Bot 2021 Apr 20. Epub 2021 Apr 20.

Biointeractions and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.

Plants have developed intricate defense mechanisms, referred to as innate immunity, to defend themselves against a wide range of pathogens. Plants often respond rapidly to pathogen attack by the synthesis and delivery of various antimicrobial compounds, proteins and small RNA in membrane vesicles to the primary infection sites. Much of the evidence regarding the importance of vesicular trafficking in plant-pathogen interactions comes from the studies involving model plants whereas this process is relatively understudied in crop plants. Read More

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Patterns of bacterial motility in microfluidics-confining environments.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Faculty of Engineering, Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada;

Understanding the motility behavior of bacteria in confining microenvironments, in which they search for available physical space and move in response to stimuli, is important for environmental, food industry, and biomedical applications. We studied the motility of five bacterial species with various sizes and flagellar architectures (, , , , and ) in microfluidic environments presenting various levels of confinement and geometrical complexity, in the absence of external flow and concentration gradients. When the confinement is moderate, such as in quasi-open spaces with only one limiting wall, and in wide channels, the motility behavior of bacteria with complex flagellar architectures approximately follows the hydrodynamics-based predictions developed for simple monotrichous bacteria. Read More

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Gene-gene interactions dictate ciprofloxacin resistance in and facilitate prediction of resistance phenotype from genome sequence data.

Antimicrob Agents Chemother 2021 Apr 19. Epub 2021 Apr 19.

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

Ciprofloxacin is one of the most widely used antibiotics for treating infections. However acquires mutations that confer ciprofloxacin resistance, making treatment more difficult. Resistance is multifactorial, with mutations in multiple genes influencing the resistance phenotype. Read More

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N-Acyl Homoserine Lactone-Mediated Quorum Sensing Regulates Species Interactions in Multispecies Biofilm Communities.

Front Cell Infect Microbiol 2021 18;11:646991. Epub 2021 Mar 18.

Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.

Bacterial biofilms are important medically, environmentally and industrially and there is a need to understand the processes that govern functional synergy and dynamics of species within biofilm communities. Here, we have used a model, mixed-species biofilm community comprised of PAO1, Pf-5 and KP1. This biofilm community displays higher biomass and increased resilience to antimicrobial stress conditions such as sodium dodecyl sulfate and tobramycin, compared to monospecies biofilm populations. Read More

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Global identification of RsmA/N binding sites in by UV CLIP-seq.

RNA Biol 2021 Apr 27:1-16. Epub 2021 Apr 27.

Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.

harbours two redundant RNA-binding proteins RsmA/RsmN (RsmA/N), which play a critical role in balancing acute and chronic infections. However, binding sites on target transcripts and the overall impact on the physiology remains unclear. In this study, we applied UV crosslinking immunoprecipitation followed by RNA-sequencing (UV CLIP-seq) to detect RsmA/N-binding sites at single-nucleotide resolution and mapped more than 500 binding sites to approximately 400 genes directly bound by RsmA/N in . Read More

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The theoretical adhesion of Pseudomonas aeruginosa and Escherichia coli on some plumbing materials in presence of distilled water or tap water.

Folia Microbiol (Praha) 2021 Apr 17. Epub 2021 Apr 17.

Bioprocess and Bio-Interfaces Laboratory, Faculty of Science and Techniques, Sultan Moulay Slimane University, BP 523, Beni Mellal, Morocco.

The main aim of this work was to determine the most appropriate materials for the installation of a water system according to the characteristics of the water that passes through it. To this end, we conducted an investigation of the effect of two types of water (SDW: sterile distilled water and STW: sterile tap water) on the properties of bacterial surfaces and the theoretical adhesion of two bacteria (Pseudomonas aeruginosa and Escherichia coli) on six plumbing materials. Contact angle measurements were used to determine the surface energies of bacteria and materials. Read More

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A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance.

Planta 2021 Apr 15;253(5):102. Epub 2021 Apr 15.

Plant-Microbe Interactions, Department of Biology, Science4Life, Utrecht University, 800.56, 3508 TB, Utrecht, The Netherlands.

Main Conclusion: Overexpression of pathogen-induced cysteine-rich transmembrane proteins (PCMs) in Arabidopsis thaliana enhances resistance against biotrophic pathogens and stimulates hypocotyl growth, suggesting a potential role for PCMs in connecting both biological processes. Plants possess a sophisticated immune system to protect themselves against pathogen attack. The defense hormone salicylic acid (SA) is an important player in the plant immune gene regulatory network. Read More

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