28,053 results match your criteria interactions basic


Pro-inflammatory and proliferative microglia drive progression of glioblastoma.

Cell Rep 2021 Sep;36(11):109718

Department of Neurosurgery, The First Medical Center of Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China. Electronic address:

Scant understanding of the glioblastoma microenvironment and molecular bases hampers development of efficient treatment strategies. Analyses of gene signatures of human gliomas demonstrate that the SETD2 mutation is correlated with poor prognosis of IDH1/2 wild-type (IDH-WT) adult glioblastoma patients. To better understand the crosstalk between SETD2 mutant (SETD2-mut) glioblastoma cells and the tumor microenvironment, we leverage single-cell transcriptomics to comprehensively map cellular populations in glioblastoma. Read More

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September 2021

Glycogen synthase kinase-3 (GSK-3) a magic enzyme: it's role in diabetes mellitus and glucose homeostasis, interactions with fluroquionlones. A mini-review.

Braz J Biol 2021 10;83:e250179. Epub 2021 Sep 10.

Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, Khyber Pakhtunkhwa, Pakistan.

Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. Read More

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September 2021

Activation of gene expression by detergent-like protein domains.

iScience 2021 Sep 21;24(9):103017. Epub 2021 Aug 21.

College of Pharmacy and Health Sciences, Butler University, Indianapolis, IN 46208, USA.

The mechanisms by which transcriptional activation domains (tADs) initiate eukaryotic gene expression have been an enigma for decades because most tADs lack specificity in sequence, structure, and interactions with targets. Machine learning analysis of data sets of tAD sequences generated elucidated several functionality rules: the functional tAD sequences should (i) be devoid of or depleted with basic amino acid residues, (ii) be enriched with aromatic and acidic residues, (iii) be with aromatic residues localized mostly near the terminus of the sequence, and acidic residues localized more internally within a span of 20-30 amino acids, (iv) be with both aromatic and acidic residues preferably spread out in the sequence and not clustered, and (v) not be separated by occasional basic residues. These and other more subtle rules are not absolute, reflecting absence of a tAD consensus sequence, enormous variability, and consistent with surfactant-like tAD biochemical properties. Read More

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September 2021

Mechanistic insights into Cd(II) and As(V) sorption on Miscanthus biochar at different pH values and pyrolysis temperatures.

Chemosphere 2021 Sep 8;287(Pt 2):132179. Epub 2021 Sep 8.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address:

Biochar has received great attention as a biosorbent, but explanations of the underlying sorption mechanisms are still unclear. Here, batch sorption of cadmium (Cd(II)) and arsenate (As(V)) to Miscanthus biochar at different pH values and pyrolysis temperatures and the sorption mechanisms were comprehensively investigated. The maximum sorption capacities for both Cd(II) and As(V) were observed under alkaline conditions. Read More

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September 2021

Tolerance to glucose and lipid high metabolic reactions after burns in an obese rat model.

J Burn Care Res 2021 Sep 14. Epub 2021 Sep 14.

Department of Epidemiology and Health Statistics, School of Public Health, Zunyi Medical University, Zunyi, Guizhou, China.

The goal of this study was to determine what effect obese body weight and a burn injury can have on the metabolism of glucose and lipids in rats. We used a 3*3 factorial model design to provide basic glucose and lipid metabolic data characterizing the interaction between different weight and burn injury groups. Two hundred Sprague Dawley (SD) rats were categorized into three weight groups (normal, overweight, obese) and then further divided into control, second degree, and third degree burn groups. Read More

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September 2021

Endocytosis of abiotic nanomaterials and nanobiovectors: Inhibition of membrane trafficking.

Nano Today 2021 Oct 8;40:101279. Epub 2021 Sep 8.

The Graduate School, Augusta University, Augusta, GA 30912, United States.

Humans are exposed to nanoscopical nanobiovectors (e.g. coronavirus SARS-CoV-2) as well as abiotic metal/carbon-based nanomaterials that enter cells serendipitously or intentionally. Read More

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October 2021

A mathematical model for the dynamics of SARS-CoV-2 virus using the Caputo-Fabrizio operator.

Math Biosci Eng 2021 07;18(5):6095-6116

Department of Mathematics and General Sciences, Prince Sultan, University, Riyadh, Saudi Arabia.

The pandemic of SARS-CoV-2 virus remains a pressing issue with unpredictable characteristics which spread worldwide through human interactions. The current study is focusing on the investigation and analysis of a fractional-order epidemic model that discusses the temporal dynamics of the SARS-CoV-2 virus in a community. It is well known that symptomatic and asymptomatic individuals have a major effect on the dynamics of the SARS-CoV-2 virus therefore, we divide the total population into susceptible, asymptomatic, symptomatic, and recovered groups of the population. Read More

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Pseudogenes and their potential functions in hematopoiesis.

Exp Hematol 2021 Sep 10. Epub 2021 Sep 10.

State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & School of Basic Medicine, Peking Union Medical College (PUMC), Beijing 100005, China; Key Laboratory of RNA and Hematopoietic Regulation, Chinese Academy of Medical Sciences, Beijing 100005, China; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. Electronic address:

Pseudogenes are DNA regions comprising defective copies of functional genes, the majority of which were generated by RNA- or DNA-level duplications. They exist across almost all forms of life and account for about one-quarter of the annotated genes in the human genome. Although they have been considered non-functional for decades, a growing number of pseudogenes have been shown to be transcribed and to play crucial regulatory roles. Read More

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September 2021

Discovery, development and application of drugs targeting BCL-2 pro-survival proteins in cancer.

Biochem Soc Trans 2021 Sep 13. Epub 2021 Sep 13.

La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.

The discovery of a new class of small molecule compounds that target the BCL-2 family of anti-apoptotic proteins is one of the great success stories of basic science leading to translational outcomes in the last 30 years. The eponymous BCL-2 protein was identified over 30 years ago due to its association with cancer. However, it was the unveiling of the biochemistry and structural biology behind it and its close relatives' mechanism(s)-of-action that provided the inspiration for what are now known as 'BH3-mimetics', the first clinically approved drugs designed to specifically inhibit protein-protein interactions. Read More

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September 2021

Interaction of Human C5a with the Major Peptide Fragments of C5aR1: Direct Evidence in Support of "Two-Site" Binding Paradigm.

ACS Omega 2021 Sep 26;6(35):22876-22887. Epub 2021 Aug 26.

Chemical Biology Laboratory, School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Odisha 752050, India.

The C5a receptor's (C5aR1) physiological function in various tissues depends on its high-affinity binding to the cationic proinflammatory glycoprotein C5a, produced during the activation of the complement system. However, an overstimulated complement can quickly alter the C5a-C5aR1 function from physiological to pathological, as has been noted in the case of several chronic inflammation-induced diseases like asthma, lung injury, multiorgan failure, sepsis, and now COVID-19. In the absence of the structural data, the current study provides the confirmatory biophysical validation of the hypothesized "two-site" binding interactions of C5a, involving (i) the N-terminus (NT) peptide ("Site1") and (ii) the extracellular loop 2 (ECL2) peptide of the extracellular surface (ECS) of the C5aR1 ("Site2"), as illustrated earlier in the reported model structural complex of C5a-C5aR1. Read More

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September 2021

Revisiting the "Paradox of Stereotaxic Surgery": Insights Into Basal Ganglia-Thalamic Interactions.

Front Syst Neurosci 2021 27;15:725876. Epub 2021 Aug 27.

Department of Neurology, University of Michigan, Ann Arbor, MI, United States.

Basal ganglia dysfunction is implicated in movement disorders including Parkinson Disease, dystonia, and choreiform disorders. Contradicting standard "rate models" of basal ganglia-thalamic interactions, internal pallidotomy improves both hypo- and hyper-kinetic movement disorders. This "paradox of stereotaxic surgery" was recognized shortly after rate models were developed, and is underscored by the outcomes of deep brain stimulation (DBS) for movement disorders. Read More

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Identification of SARS-CoV-2 inhibitors from extracts of Thunb.

Saudi J Biol Sci 2021 Sep 6. Epub 2021 Sep 6.

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Saudi Arabia.

Thunb., a perennial herb belonging to the Saururaceae family is a well-known ingredient of Traditional Chinese medicine (TCM) with several therapeutic properties. During the severe acute respiratory syndrome (SARS) outbreak in China, it was one of the approved ingredients in SARS preventative formulations and therefore, the plant may contain novel bioactive chemicals that can be used to suppress the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a virus for which there are currently no effective drugs available. Read More

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September 2021

Upregulated lncRNA Cyclin-dependent kinase inhibitor 2B antisense RNA 1 induces the proliferation and migration of colorectal cancer by miR-378b/CAPRIN2 axis.

Bioengineered 2021 Dec;12(1):5476-5490

Department of Gastrointestinal Surgery, Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University, Fuzhou, China.

LncRNA Cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) plays a role in the progression of multiple cancers like cholangiocarcinoma, osteosarcoma and several gastrointestinal tumors. Few studies have linked its function and mechanism to the development of colorectal cancer (CRC). The expression of CDKN2B-AS1, microRNA (miR)-378b, and cytoplasmic activation/proliferation-associated protein 2 (CAPRIN2) was analyzed in CRC patients and cell lines. Read More

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December 2021

A three-dimensional bioprinting technique, based on a gelatin/alginate hydrogel, for the tissue engineering of hair follicle reconstruction.

Int J Biol Macromol 2021 Sep 9. Epub 2021 Sep 9.

Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address:

Hair loss remains a challenging clinical problem that influences the quality of life. Three-dimensional (3D) bioprinting has become a valuable tool for fabricating tissue constructs for transplantation and other biomedical applications. Although some simple organs, such as skin and cartilage, have been successfully simulated, it remains challenging to make hair follicles (HFs), which are highly complex organs. Read More

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September 2021

The evolution of evolutionary processes in organismal and cancer evolution.

Authors:
Frank H Laukien

Prog Biophys Mol Biol 2021 Sep 9. Epub 2021 Sep 9.

Bruker Corporation, 40 Manning Road, Billerica, MA, 01821, USA; Visiting Scholar, Harvard University, Origins of Life Initiative, Department of Chemistry & Chemical Biology, 12 Oxford Street, Cambridge, MA, 02138, USA; Member, The Oncology Think Tank (TOTT), Columbia University, New York, NY, USA. Electronic address:

The evolution of early life and of contemporary viruses has been driven in significant part by random genetic mutations, while modern unicellular and organismal evolution primarily leverages evolved, efficient and active cell biology processes for adaptive changes prior to selection. Random mutations are often buffered by cell homeostasis, or they have a negative role, e.g. Read More

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September 2021

Spectroscopic, calorimetric and in silico insight into the molecular interactions of Memantine with human transferrin: Implications of Alzheimer's drugs.

Int J Biol Macromol 2021 Sep 8;190:660-666. Epub 2021 Sep 8.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India. Electronic address:

Human transferrin (Tf) is an iron-binding blood plasma glycoprotein that controls free iron in biological fluids. Tf is a liver-produced protein that binds iron very tightly but reversibly and is the most significant iron pool. Memantine is an orally administrative N-methyl-d-aspartate glutamate receptor antagonist used to slow the progression of moderate-to-severe Alzheimer's disease (AD) and dementia. Read More

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September 2021

EP300/CBP is crucial for cAMP-PKA pathway to alleviate podocyte dedifferentiation via targeting Notch3 signaling.

Exp Cell Res 2021 Sep 10;407(2):112825. Epub 2021 Sep 10.

Department of Pathology, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, 200032, China; Shanghai Key Laboratory of Kidney Disease and Blood Purification, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. Electronic address:

Podocyte injury is the hallmark of proteinuric glomerular diseases. Notch3 is neo-activated simultaneously in damaged podocytes and podocyte's progenitor cells of FSGS, indicating a unique role of Notch3. We previously showed that activation of cAMP-PKA pathway alleviated podocyte injury possibly via inhibiting Notch3 expression. Read More

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September 2021

Networks of Causal Linkage Between Eigenmodes Characterize Behavioral Dynamics of Caenorhabditis elegans.

PLoS Comput Biol 2021 Sep 10;17(9):e1009329. Epub 2021 Sep 10.

Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California, United States of America.

Behavioral phenotyping of model organisms has played an important role in unravelling the complexities of animal behavior. Techniques for classifying behavior often rely on easily identified changes in posture and motion. However, such approaches are likely to miss complex behaviors that cannot be readily distinguished by eye (e. Read More

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September 2021

Healthcare professionals' practice and interactions in older peoples' cross-sectoral clinical care trajectories when acutely hospitalized - a qualitative observation study.

BMC Health Serv Res 2021 Sep 9;21(1):944. Epub 2021 Sep 9.

Research Unit for Health Promotion, Department of Public Health, University of Southern Denmark, Degnevej 14, 6705, Esbjerg, Denmark.

Background: Healthcare services have become more complex, globally and nationally. Denmark is renowned for an advanced and robust healthcare system, aiming at a less fragmented structure. However, challenges within the coordination of care remain. Read More

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September 2021

Interfacial Water Structure of Binary Liquid Mixtures Reflects Nonideal Behavior.

J Phys Chem B 2021 Sep 10. Epub 2021 Sep 10.

Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.

The evaporation of molecules from water-organic solute binary mixtures is key for both atmospheric and industrial processes such as aerosol formation and distillation. Deviations from ideal evaporation energetics can be assigned to intermolecular interactions in solution, yet evaporation occurs from the interface, and the poorly understood interfacial, rather than the bulk, structure of binary mixtures affects evaporation kinetics. Here we determine the interfacial structure of nonideal binary mixtures of water with methanol, ethanol, and formic acid, by combining surface-specific vibrational spectroscopy with molecular dynamics simulations. Read More

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September 2021

A Novel Regulatory Player in the Innate Immune System: Long Non-Coding RNAs.

Int J Mol Sci 2021 Sep 2;22(17). Epub 2021 Sep 2.

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Long non-coding RNAs (lncRNAs) represent crucial transcriptional and post-transcriptional gene regulators during antimicrobial responses in the host innate immune system. Studies have shown that lncRNAs are expressed in a highly tissue- and cell-specific- manner and are involved in the differentiation and function of innate immune cells, as well as inflammatory and antiviral processes, through versatile molecular mechanisms. These lncRNAs function via the interactions with DNA, RNA, or protein in either cis or trans pattern, relying on their specific sequences or their transcriptions and processing. Read More

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September 2021

Mechanisms of Binding Specificity among bHLH Transcription Factors.

Int J Mol Sci 2021 Aug 24;22(17). Epub 2021 Aug 24.

Neurogenomics Group, Research Programme on Biomedical Informatics (GRIB), Hospital del Mar Medical Research Institute (IMIM), DCEXS, Universitat Pompeu Fabra, 08003 Barcelona, Spain.

The transcriptome of every cell is orchestrated by the complex network of interaction between transcription factors (TFs) and their binding sites on DNA. Disruption of this network can result in many forms of organism malfunction but also can be the substrate of positive natural selection. However, understanding the specific determinants of each of these individual TF-DNA interactions is a challenging task as it requires integrating the multiple possible mechanisms by which a given TF ends up interacting with a specific genomic region. Read More

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Possible Link between Higher Transmissibility of Alpha, Kappa and Delta Variants of SARS-CoV-2 and Increased Structural Stability of Its Spike Protein and hACE2 Affinity.

Int J Mol Sci 2021 Aug 24;22(17). Epub 2021 Aug 24.

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology (IIT) Delhi, New Delhi 110016, India.

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak in December 2019 has caused a global pandemic. The rapid mutation rate in the virus has created alarming situations worldwide and is being attributed to the false negativity in RT-PCR tests. It has also increased the chances of reinfection and immune escape. Read More

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Local Piezoelectric Properties of Doped Biomolecular Crystals.

Materials (Basel) 2021 Aug 30;14(17). Epub 2021 Aug 30.

Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Herzl St 234, Rehovot 7610001, Israel.

Piezoelectricity is the ability of certain crystals to generate mechanical strain proportional to an external electric field. Though many biomolecular crystals contain polar molecules, they are frequently centrosymmetric, signifying that the dipole moments of constituent molecules cancel each other. However, piezoelectricity can be induced by stereospecific doping leading to symmetry reduction. Read More

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Insights into Conformational Dynamics and Allostery in DNMT1-H3Ub/USP7 Interactions.

Molecules 2021 Aug 25;26(17). Epub 2021 Aug 25.

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

DNA methyltransferases (DNMTs) including DNMT1 are a conserved family of cytosine methylases that play crucial roles in epigenetic regulation. The versatile functions of DNMT1 rely on allosteric networks between its different interacting partners, emerging as novel therapeutic targets. In this work, based on the modeling structures of DNMT1-ubiquitylated H3 (H3Ub)/ubiquitin specific peptidase 7 (USP7) complexes, we have used a combination of elastic network models, molecular dynamics simulations, structural residue perturbation, network modeling, and pocket pathway analysis to examine their molecular mechanisms of allosteric regulation. Read More

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Carotenoids Do Not Protect Bacteriochlorophylls in Isolated Light-Harvesting LH2 Complexes of Photosynthetic Bacteria from Destructive Interactions with Singlet Oxygen.

Molecules 2021 Aug 24;26(17). Epub 2021 Aug 24.

Institute of Basic Biological Problems RAS, 142290 Pushchino, Russia.

The effect of singlet oxygen on light-harvesting (LH) complexes has been studied for a number of sulfur (S) and nonsulfur (S) photosynthetic bacteria. The visible/near-IR absorption spectra of the standard LH2 complexes (B800-850) of (.) (S), (. Read More

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Exploring the global vaginal microbiome and its impact on human health.

Microb Pathog 2021 Sep 6:105172. Epub 2021 Sep 6.

Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France; IHU-Méditerranée Infection, Marseille, France. Electronic address:

Around the world, more than 175,000,000 women are diagnosed every year with gynaecological disease, in many cases contributing to high morbidity and mortality. For this reason, knowledge of the composition of the vaginal microbiome and its variations represents a real health challenge, as this is key to improving therapeutic management. This review traces the history of the poorly known vaginal microbiome and focuses on the latest findings concerning this ecosystem. Read More

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September 2021

Nucleolar localization of c-Jun.

FEBS J 2021 Sep 9. Epub 2021 Sep 9.

Department of Biology, York University, Toronto, ON, M3J 1P3, Canada.

Nucleoli are well-defined for their function in ribosome biogenesis but only a small fraction of the nucleolar proteome has been characterized. Here, we report that the proto-oncogene, c-Jun, is targeted to the nucleolus. Using live cell imaging in myogenic cells, we document that the c-Jun basic domain contains a unique, evolutionarily conserved motif that determines nucleolar targeting. Read More

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September 2021

Determining the Phase Separation Characteristics of Plant Proteins.

Curr Protoc 2021 Sep;1(9):e237

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Eukaryotic cells contain numerous membrane-bound and membraneless organelles that provide spatiotemporal control for diverse biological processes. The liquid-liquid phase separation of proteins has been proposed as the driving force behind the formation of membraneless organelles. Here, we describe a method to determine the phase separation activities of proteins in plants. Read More

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September 2021

Using Biotinylated -Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate-Protein Interactions with Surface-Based Biosensors.

Biochemistry 2021 Sep 9. Epub 2021 Sep 9.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125 Berlin, Germany.

Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins to regulate biological functions, such as protein-protein interactions. PP-InsPs are strongly negatively charged and interact with highly basic surface patches in proteins, making their quantitative biochemical analysis challenging. Read More

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September 2021