646 results match your criteria interaction seeding

NMR unveils an N-terminal interaction interface on acetylated-α-synuclein monomers for recruitment to fibrils.

Proc Natl Acad Sci U S A 2021 May;118(18)

Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854

Amyloid fibril formation of α-synuclein (αS) is associated with multiple neurodegenerative diseases, including Parkinson's disease (PD). Growing evidence suggests that progression of PD is linked to cell-to-cell propagation of αS fibrils, which leads to seeding of endogenous intrinsically disordered monomer via templated elongation and secondary nucleation. A molecular understanding of the seeding mechanism and driving interactions is crucial to inhibit progression of amyloid formation. Read More

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Synthesis of FAU-Zeolite Membrane by a Secondary Growth Method: Influence of Seeding on Membrane Growth and Its Performance in the Dehydration of Isopropyl Alcohol-Water Mixture.

ACS Omega 2021 Apr 31;6(14):9834-9842. Epub 2021 Mar 31.

Department of Applied Chemistry, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Y-type zeolite membranes were prepared on a porous tubular α-alumina support by a secondary growth process. Various experimental conditions such as seed size, pH of seed solution, and degassing of support were examined for understanding their influence on the membrane deposition process. The experimental results showed that the potential of alumina support surface and the USY seed slurry plays a significant role in controlling the electrostatic interaction between seed particles and support surface and also the aggregation of USY seed particles in the slurry. Read More

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In situ architecture of neuronal α-Synuclein inclusions.

Nat Commun 2021 04 14;12(1):2110. Epub 2021 Apr 14.

Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

The molecular architecture of α-Synuclein (α-Syn) inclusions, pathognomonic of various neurodegenerative disorders, remains unclear. α-Syn inclusions were long thought to consist mainly of α-Syn fibrils, but recent reports pointed to intracellular membranes as the major inclusion component. Here, we use cryo-electron tomography (cryo-ET) to image neuronal α-Syn inclusions in situ at molecular resolution. Read More

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Myeloid cell-derived PROS1 inhibits tumor metastasis by regulating inflammatory and immune responses via IL-10.

J Clin Invest 2021 Apr 13. Epub 2021 Apr 13.

Institute of Dental Sciences, Hebrew University of Jerusalem, Jerusalem, Israel.

Stimulation of TAM (TYRO3, AXL and MERTK) Receptor Tyrosine Kinases promotes tumor progression through numerous cellular mechanisms. TAM cognate ligands GAS6 and PROS1 (for TYRO3 and MERTK) are secreted by host immune cells, an interaction which may support tumor progression. Here we reveal an unexpected anti-metastatic role for myeloid-derived PROS1, directly suppressing the metastatic potential of lung and breast tumor models. Read More

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Sowing methods and seeding rates effects on yield and yield components of Tef( [Zucc.] Trotter) at Adet, North West Ethiopia.

Heliyon 2021 Mar 20;7(3):e06519. Epub 2021 Mar 20.

College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar, Ethiopia.

Tef is one of the most important staple cereal crops in Ethiopia. However, the production and productivity of the crop remain low due to lack of appropriate agronomic recommendations. Lack of optimum sowing methods and seed rates are key agronomic factors limit the productivities of the crop. Read More

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Interactions between magnetic particles and polyaluminum chloride on the coagulation behavior in humic acid-kaolin synthetic water treatment.

Environ Res 2021 Apr 1;197:111093. Epub 2021 Apr 1.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China. Electronic address:

Understanding the interactions between magnetic particles (MPs) and polyaluminum chloride (PACl) is essential to elucidate the magnetic seeding coagulation (MSC) process. However, little is known about how MPs interact with the different Al species coexisting in the PACl. Here, the relationships among pollutants removal, residual Al distribution, and floc properties were comparatively studied in the MSC and traditional coagulation (TC) processes to address this issue. Read More

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Sequential seeding in multilayer networks.

Chaos 2021 Mar;31(3):033130

Department of Computational Intelligence, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland.

Multilayer networks are the underlying structures of multiple real-world systems where we have more than one type of interaction/relation between nodes: social, biological, computer, or communication, to name only a few. In many cases, they are helpful in modeling processes that happen on top of them, which leads to gaining more knowledge about these phenomena. One example of such a process is the spread of influence. Read More

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Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation.

Micromachines (Basel) 2021 Mar 20;12(3). Epub 2021 Mar 20.

Department of Applied Physics, Science for Life Laboratory, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.

In cancer research and drug screening, multicellular tumor spheroids (MCTSs) are a popular model to bridge the gap between in vitro and in vivo. However, the current techniques to culture mixed co-culture MCTSs do not mimic the structural architecture and cellular spatial distribution in solid tumors. In this study we present an acoustic trapping-based core-shell MCTSs culture method using sequential seeding of the core and shell cells into microwells coated with a protein repellent coating. Read More

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Construction of dominant rice population under dry cultivation by seeding rate and nitrogen rate interaction.

Sci Rep 2021 Mar 30;11(1):7189. Epub 2021 Mar 30.

Agronomy College, Jilin Agricultural University, Changchun, 130118, Jilin, China.

This study used the rice cultivar Suijing 18 to investigate the effects of morphological characteristics, photosynthetic changes, yield, as well as nitrogen absorption and utilization. The interaction between seeding rate and nitrogen rate was also assessed to identify the most suitable values of the dominant population for both factors under dry cultivation. Furthermore, the photosynthetic physiological characteristics of the upper three leaves in the dominant population were also explored. Read More

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Bone marrow niches in the regulation of bone metastasis.

Br J Cancer 2021 Mar 23. Epub 2021 Mar 23.

Department of Molecular Biology, Princeton University, Princeton, NJ, USA.

The bone marrow has been widely recognised to host a unique microenvironment that facilitates tumour colonisation. Bone metastasis frequently occurs in the late stages of malignant diseases such as breast, prostate and lung cancers. The biology of bone metastasis is determined by tumour-cell-intrinsic traits as well as their interaction with the microenvironment. Read More

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The Charge-State and Structural Stability of Peptides Conferred by Microsolvating Environments in Differential Mobility Spectrometry.

J Am Soc Mass Spectrom 2021 Apr 18;32(4):956-968. Epub 2021 Mar 18.

Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo N2L 3G1, Ontario, Canada.

The presence of solvent vapor in a differential mobility spectrometry (DMS) cell creates a microsolvating environment that can mitigate complications associated with field-induced heating. In the case of peptides, the microsolvation of protonation sites results in a stabilization of charge density through localized solvent clustering, sheltering the ion from collisional activation. Seeding the DMS carrier gas (N) with a solvent vapor prevented nearly all field-induced fragmentation of the protonated peptides GGG, AAA, and the Lys-rich Polybia-MP1 (IDWKKLLDAAKQIL-NH). Read More

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Dual amyloid cross-seeding reveals steric zipper-facilitated fibrillization and pathological links between protein misfolding diseases.

J Mater Chem B 2021 Apr 2;9(15):3300-3316. Epub 2021 Mar 2.

Department of Chemical, Biomolecular, and Corrosion Engineering The University of Akron, Ohio, USA.

Amyloid cross-seeding, as a result of direct interaction and co-aggregation between different disease-causative peptides, is considered as a main mechanism for the spread of the overlapping pathology across different cells and tissues between different protein-misfolding diseases (PMDs). Despite the biomedical significance of amyloid cross-seeding in amyloidogenesis, it remains a great challenge to discover amyloid cross-seeding systems and reveal their cross-seeding structures and mechanisms. Herein, we are the first to report that GNNQQNY - a short fragment from yeast prion protein Sup35 - can cross-seed with both amyloid-β (Aβ, associated with Alzheimer's disease) and human islet amyloid polypeptide (hIAPP, associated with type II diabetes) to form β-structure-rich assemblies and to accelerate amyloid fibrillization. Read More

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Amyloid cross-sequence interaction between Aβ(1-40) and αA(66-80) in relation to the pathogenesis of cataract.

Int J Biol Macromol 2021 May 21;179:61-70. Epub 2021 Feb 21.

Ulm University, Institute of Protein Biochemistry, Helmholtzstraße 8/1, 89081 Ulm, Germany; Department of Biotechnology, Central University of Rajasthan, NH-8 Bandarsindri, Kishangarh, Ajmer 305817, Rajasthan, India. Electronic address:

Alzheimer's disease (AD) and cataract represent two common protein misfolding diseases closely associated with aging. Growing evidence suggests that these two diseases may be interrelated with each other through cross-sequence interactions between β-amyloid (Aβ) peptide and the short aggregating peptides derived from proteolytic breakdown of α-crystallin. αΑ(66-80) is one of several peptides produced by the proteolytic breakdown of α-crystallin in aged eye lens. Read More

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Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay.

J Immunol Methods 2021 May 18;492:113000. Epub 2021 Feb 18.

Blood and Coagulation Research, US Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, United States of America. Electronic address:

Background: Immunomodulation by mesenchymal stromal cells (MSCs) is a potentially important therapeutic modality. MSCs suppress peripheral blood mononuclear cell (PBMC) proliferation in vitro, suggesting a mechanism for suppressing inflammatory responses in vivo. This study details the interactions of PBMCs and MSCs. Read More

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Peripheral sensory neurons promote angiogenesis in neurovascular models derived from hESCs.

Stem Cell Res 2021 Apr 10;52:102231. Epub 2021 Feb 10.

Faculty of Dentistry, National University of Singapore, Singapore. Electronic address:

In the adult tissues, blood vessels traverse the body with neurons side by side; and share common signaling molecules. Developmental studies on animal models have shown that peripheral sensory neurons (PSNs) secrete angiogenic factors and endothelial cells (ECs) secrete neurotrophic factors which contribute to their coexistence, thereby forming the peripheral neurovascular (PNV) unit. Despite the large number of studies showing that innervation and vascularization complement each other, the interaction between human PSNs and ECs is still largely unknown. Read More

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Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

J Clin Invest 2021 Feb;131(4)

Department of Pharmaceutical Sciences, University of South Florida, Tampa, Florida, USA.

Tauopathies display a spectrum of phenotypes from cognitive to affective behavioral impairments; however, mechanisms promoting tau pathology and how tau elicits behavioral impairment remain unclear. We report a unique interaction between polyamine metabolism, behavioral impairment, and tau fate. Polyamines are ubiquitous aliphatic molecules that support neuronal function, axonal integrity, and cognitive processing. Read More

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February 2021

Sub-10 nm Substrate Roughness Promotes the Cellular Uptake of Nanoparticles by Upregulating Endocytosis-Related Genes.

Nano Lett 2021 02 15;21(4):1839-1847. Epub 2021 Feb 15.

Nanosubstrate engineering is an established approach for modulating cellular responses, but it remains infrequently exploited to facilitate the intracellular delivery of nanoparticles (NPs). We report nanoscale roughness of the extracellular environment as a critical parameter for regulating the cellular uptake of NPs. After seeding cells atop a substrate that contains randomly immobilized gold NPs (termed AuNP-S) with sub-10 nm surface roughness, we demonstrate that such cells internalize up to ∼100-fold more poly(ethylene glycol)-coated AuNPs (Au@PEG NPs) than those cells seeded on a conventional flat culture plate. Read More

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February 2021

Metastatic Dissemination Mimicked in a Multicellular Transwell Assay.

Methods Mol Biol 2021 ;2235:181-190

Vascular Oncology and Metastasis, German Cancer Research Center Heidelberg (DKFZ-ZMBH Alliance), Heidelberg, Germany.

Tumor progression and metastasis are multistep processes that are critically dependent on the interaction of metastasizing tumor cells with other cells of the local microenvironment. Mimicking the single steps of the metastatic cascade in vitro is therefore challenging when investigating not only tumor cell behavior alone but also cellular crosstalk between different cell populations. In particular, the crosstalk of tumor cells with pericytes and endothelial cells when accessing the bloodstream is of great importance for successful intravasation and metastatic dissemination. Read More

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The expanding scope of amyloid signalling.

Prion 2021 Dec;15(1):21-28

Institut de Biochimie et de Génétique Cellulaire (CNRS UMR 5095, Université de Bordeaux) , France.

Formation of higher-order supramolecular complexes has emerged as a common principle underlying activity of a number of immune and regulated cell-death signalling pathways in animals, plants and fungi. Some of these signalosomes employ functional amyloid motifs in their assembly process. The description of such systems in fungi finds its origin in earlier studies on a fungal prion termed [Het-s], originally identified as a non-Mendelian cytoplasmic infectious element. Read More

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December 2021

Partial Prion Cross-Seeding between Fungal and Mammalian Amyloid Signaling Motifs.

mBio 2021 02 9;12(1). Epub 2021 Feb 9.

Non-Self Recognition in Fungi, Institut de Biochimie et de Génétique Cellulaire (CNRS UMR 5095), Université de Bordeaux, Bordeaux, France

In filamentous fungi, NLR-based signalosomes activate downstream membrane-targeting cell death-inducing proteins by a mechanism of amyloid templating. In the species , two such signalosomes, NWD2/HET-S and FNT1/HELLF, have been described. An analogous system involving a distinct amyloid signaling motif, termed PP, was also identified in the genome of the species and studied using heterologous expression in The PP motif bears resemblance to the RIP homotypic interaction motif (RHIM) and to RHIM-like motifs controlling necroptosis in mammals and innate immunity in flies. Read More

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February 2021

Fully absorbable poly-4-hydroxybutyrate implants exhibit more favorable cell-matrix interactions than polypropylene.

Mater Sci Eng C Mater Biol Appl 2021 Jan 11;120:111702. Epub 2020 Nov 11.

Department of Obstetrics and Gynecology, Amsterdam Reproduction and Development, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam, the Netherlands. Electronic address:

Pelvic organ prolapse (POP) is a multifactorial condition characterized by the descent of the pelvic organs due to the loss of supportive tissue strength. This is presumably caused by the decreased fibroblast function and the subsequent change in the quality of the extracellular matrix. The correction of POP using an implant intends to provide mechanical support to the pelvic organs and to stimulate a moderate host response. Read More

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January 2021

Title: insoluble proteins catch heterologous soluble proteins into inclusion bodies by intermolecular interaction of aggregating peptides.

Microb Cell Fact 2021 Feb 2;20(1):30. Epub 2021 Feb 2.

Institute for Biotechnology and Biomedicine, Autonomous University of Barcelona, 08193, Bellaterra, Barcelona, Spain.

Background: Protein aggregation is a biological event observed in expression systems in which the recombinant protein is produced under stressful conditions surpassing the homeostasis of the protein quality control system. In addition, protein aggregation is also related to conformational diseases in animals as transmissible prion diseases or non-transmissible neurodegenerative diseases including Alzheimer, Parkinson's disease, amyloidosis and multiple system atrophy among others. At the molecular level, the presence of aggregation-prone domains in protein molecules act as seeding igniters to induce the accumulation of protein molecules in protease-resistant clusters by intermolecular interactions. Read More

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February 2021

The association of circulating amylin with β-amyloid in familial Alzheimer's disease.

Alzheimers Dement (N Y) 2021 20;7(1):e12130. Epub 2021 Jan 20.

Department of Pharmacology and Nutritional Sciences University of Kentucky Lexington Kentucky USA.

Introduction: This study assessed the hypothesis that circulating human amylin (amyloid-forming) cross-seeds with amyloid beta (Aβ) in early Alzheimer's disease (AD).

Methods: Evidence of amylin-AD pathology interaction was tested in brains of 31 familial AD mutation carriers and 20 cognitively unaffected individuals, in cerebrospinal fluid (CSF) (98 diseased and 117 control samples) and in genetic databases. For functional testing, we genetically manipulated amylin secretion in APP/PS1 and non-APP/PS1 rats. Read More

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January 2021

Biocompatibility Test of Multiwall Carbon Nanotubes with Human Osteoblast Cells: Potential Application for Bone Implant Interface Reinforcement.

J Nanosci Nanotechnol 2021 Apr;21(4):2394-2403

Department of Oral Biology and Experimental Dental Research University of Szeged, Faculty of Dentistry, H-6720 Szeged, Tisza Lajos krt. 64., Hungary.

Application of multiwall carbon nanotubes (MWCNT) as a filler component in composite materials can lead to remarkable increase in mechanical strength. It is a challenging application to form a living bone tissue biocomposite that is reinforced with MWCNTs at a dental implant-bone interface. The successful biointegration of MWCNT and the implant material depends on the processes of osseointegration, namely surface interactions at the molecular and cellular level. Read More

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Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

J Biol Chem 2020 11 24:100121. Epub 2020 Nov 24.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, China;. Electronic address:

Cancer stem-like cells (CSCs) contribute to the high rate of tumor heterogeneity, metastasis, therapeutic resistance, and recurrence. Histone lysine demethylase 4D (KDM4D or JMJD2D) is highly expressed in colon and liver tumors, where it promotes cancer progression; however, the role of JMJD2D in CSCs remains unclear. Here, we show that JMJD2D expression was increased in liver cancer stem-like cells (LCSCs); downregulation of JMJD2D inhibited the self-renewal of LCSCs in vitro and in vivo and inhibited the lung metastasis of LCSCs by reducing the survival and the early lung seeding of circulating LCSCs. Read More

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November 2020

Gene expression profiles of human adipose-derived mesenchymal stem cells dynamically seeded on clinically available processed nerve allografts and collagen nerve guides.

Neural Regen Res 2021 Aug;16(8):1613-1621

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.

It was hypothesized that mesenchymal stem cells (MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance® Nerve Grafts or NeuraGen® Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance® Nerve Grafts and 30 NeuraGen® Nerve Guides for 12 hours. Read More

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The Roles of Stroma-Derived Chemokine in Different Stages of Cancer Metastases.

Front Immunol 2020 22;11:598532. Epub 2020 Dec 22.

Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States.

The intricate interplay between malignant cells and host cellular and non-cellular components play crucial role in different stages of tumor development, progression, and metastases. Tumor and stromal cells communicate to each other through receptors such as integrins and secretion of signaling molecules like growth factors, cytokines, chemokines and inflammatory mediators. Chemokines mediated signaling pathways have emerged as major mechanisms underlying multifaceted roles played by host cells during tumor progression. Read More

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December 2020

Magnetic seeding coagulation: Effect of Al species and magnetic particles on coagulation efficiency, residual Al, and floc properties.

Chemosphere 2021 Apr 17;268:129363. Epub 2020 Dec 17.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China. Electronic address:

Magnetic seeding coagulation (MSC) process has been used to accelerate flocs sedimentation with an applied magnetic field, offering large handling capacity and low energy consumption. The interactions of three typical Al species, aluminum chloride (AlCl), AlO(OH) polymer (Al), and (AlO)Al(OH) polymer (Al), with magnetic particles (MPs) were examined to clarify the MSC process. In traditional coagulation (TC) process, the aggregation of primary Al-dissolved organic matter (DOM) complexes with in-situ-formed polynuclear species generated a large average floc size (226 μm), which was proved to be efficient for DOC removal (52. Read More

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Unpatterned Bioactive Poly(Butylene 1,4-Cyclohexanedicarboxylate)-Based Film Fast Induced Neuronal-Like Differentiation of Human Bone Marrow-Mesenchymal Stem Cells.

Int J Mol Sci 2020 Dec 4;21(23). Epub 2020 Dec 4.

Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy.

Herein, we present poly(butylene 1,4-cyclohexanedicarboxylate) (PBCE) films characterized by an unpatterned microstructure and a specific hydrophobicity, capable of boosting a drastic cytoskeleton architecture remodeling, culminating with the neuronal-like differentiation of human bone marrow-mesenchymal stem cells (hBM-MSCs). We have used two different filming procedures to prepare the films, solvent casting (PBCE) and compression-moulding (PBCE*). PBCE film had a rough and porous surface with spherulite-like aggregations (Ø = 10-20 μm) and was characterized by a water contact angle = 100°. Read More

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December 2020

Multi-year weed community dynamics and rice yields as influenced by tillage, crop establishment, and weed control: Implications for rice-maize rotations in the eastern Gangetic plains.

Crop Prot 2020 Dec;138:105334

International Maize and Wheat Improvement Centre (CIMMYT), Sustainable Intensification Program, House 10/B. Road 53, Gulshan-2, Dhaka, 1213, Bangladesh.

In South Asia's rice-based cropping systems, most farmers flood and repetitively till their fields before transplanting. This establishment method, commonly termed puddled transplanted rice (TPR), is costly. In addition, it is labor and energy intensive. Read More

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December 2020