73,441 results match your criteria interacting protein


Organophosphorus pesticides exert estrogen receptor agonistic effect determined using Organization for Economic Cooperation and Development PBTG455, and induce estrogen receptor-dependent adipogenesis of 3T3-L1 adipocytes.

Environ Pollut 2021 Apr 10;283:117090. Epub 2021 Apr 10.

Department of Food Science and Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea. Electronic address:

Various chemicals containing pesticides can induce adipogenesis and cause obesity. Organophosphorus pesticides have been used for pest control. Here, we investigated the estrogen receptor (ER)-dependent adipogenesis-inducing effect of representative organophosphorus pesticides (OPs), diazinon, phoxim, terbufos and tolclofos-methyl in 3T3-L1 adipocytes. Read More

View Article and Full-Text PDF

Frontiers in PROTACs.

Drug Discov Today 2021 Apr 16. Epub 2021 Apr 16.

School ofBiological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK. Electronic address:

Targeting protein-protein interactions (PPI) is a key focus in the development of new and emerging small-molecule therapeutics. Shallow interacting surfaces can render PPI targeting notoriously difficult. This leaves many therapeutically captivating targets 'undruggable'. Read More

View Article and Full-Text PDF

Nimodipine inhibits intestinal and aortic smooth muscle contraction by regulating Ca-activated Cl channels.

Toxicol Appl Pharmacol 2021 Apr 16:115543. Epub 2021 Apr 16.

Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, School of Life Sciences, Liaoning Normal University, Dalian 116000, PR China. Electronic address:

Nimodipine is a clinically used dihydropyridine L-type calcium channel antagonist that effectively inhibits transmembrane Ca influx following the depolarization of smooth muscle cells, but the detailed effect on smooth muscle contraction is not fully understood. Ca-activated Cl channels (CaCCs) in vascular smooth muscle cells (VSMCs) may regulate vascular contractility. We found that nimodipine can inhibit transmembrane protein 16A (TMEM16A) activity in a concentration-dependent manner by cell-based fluorescence-quenching assay and short-circuit current analysis, with an IC value of ~5 μM. Read More

View Article and Full-Text PDF

The actin nucleation factors JMY and WHAMM enable a rapid Arp2/3 complex-mediated intrinsic pathway of apoptosis.

PLoS Genet 2021 Apr 19;17(4):e1009512. Epub 2021 Apr 19.

Department of Molecular and Cell Biology, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, United States of America.

The actin cytoskeleton is a well-known player in most vital cellular processes, but comparably little is understood about how the actin assembly machinery impacts programmed cell death pathways. In the current study, we explored roles for the human Wiskott-Aldrich Syndrome Protein (WASP) family of actin nucleation factors in DNA damage-induced apoptosis. Inactivation of each WASP-family gene revealed that two of them, JMY and WHAMM, are necessary for rapid apoptotic responses. Read More

View Article and Full-Text PDF

Acinar cell NLRP3 inflammasome and GSDMD activation mediates pyroptosis and systemic inflammation in acute pancreatitis.

Br J Pharmacol 2021 Apr 19. Epub 2021 Apr 19.

Center of Severe Acute Pancreatitis (CASP), Department of Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background And Purpose: Pyroptosis is a lytic form of proinflammatory cell death characterised as caspase-1-dependent with canonical NLRP3 inflammasome-induced gasdermin D (GSDMD) activation. We aimed to investigate the role of acinar pyroptotic cell death in pancreatic injury and systemic inflammation in acute pancreatitis (AP).

Experimental Approach: Pancreatic acinar pyroptotic cell death pathway activation upon pancreatic toxin stimulation in vitro and in vivo were investigated. Read More

View Article and Full-Text PDF

Enterovirus-A71 exploits peripherin and Rac1 to invade the central nervous system.

EMBO Rep 2021 Apr 19:e51777. Epub 2021 Apr 19.

Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Enterovirus-A71 (EV-A71) has been associated with severe neurological forms of hand, foot, and mouth disease (HFMD). EV-A71 infects motor neurons at neuromuscular junctions (NMJs) to invade the central nervous system (CNS). Here, we investigate the role of peripherin (PRPH) during EV-A71 infection, a type III intermediate neurofilament involved in neurodegenerative conditions. Read More

View Article and Full-Text PDF

LIM domain only 1: an oncogenic transcription cofactor contributing to the tumorigenesis of multiple cancer types.

Chin Med J (Engl) 2021 Apr 16. Epub 2021 Apr 16.

Department of Medical Oncology, Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, China Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA.

Abstract: The LIM domain only 1 (LMO1) gene belongs to the LMO family of genes that encodes a group of transcriptional cofactors. This group of transcriptional cofactors regulates gene transcription by acting as a key "connector" or "scaffold" in transcription complexes. All LMOs, including LMO1, are important players in the process of tumorigenesis. Read More

View Article and Full-Text PDF

Pharmacological Properties of Violacein at the Human Serotonin 2C Receptor.

EC Pharmacol Toxicol 2019 Jan 1;30(Suppl 1):103-111. Epub 2016 Apr 1.

Department of Biological Sciences, Tennessee State University, United States of America.

The monoamine neurotransmitter serotonin (5-HT) plays a role in many physiological responses by interacting with various receptor subtypes. The 5-HT receptor subtype is a 7-transmembrane, G protein-coupled receptor (GPCR) that is involved in neuronal excitability, spatial learning, mood, and appetite. The microorganism produces a purple pigment, violacein, which can be extracted and purified. Read More

View Article and Full-Text PDF
January 2019

PLK1-mediated S369 phosphorylation of RIPK3 during G2 and M phases enables its ripoptosome incorporation and activity.

Authors:
Kartik Gupta Bo Liu

iScience 2021 Apr 17;24(4):102320. Epub 2021 Mar 17.

Division of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI 53705, USA.

Receptor-interacting protein kinase 3 executes a form of regulated necrosis called necroptosis. Upon induction of an altered conformation by chemical inhibitors or via mutations in its kinase site, RIPK3 associates with a multiprotein complex called the ripoptosome-a signaling platform containing FADD, RIPK1, caspase 8, and cFLIP-and becomes decisive in the execution of apoptosis. Surprisingly, in contexts not completely understood, the ripoptosome itself cleaves RIPK3, highlighting an apparent conundrum on how RIPK3 fulfills its role via the complex responsible for its own degradation. Read More

View Article and Full-Text PDF

Identification of a Gene Prognostic Signature for Oral Squamous Cell Carcinoma by RNA Sequencing and Bioinformatics.

Biomed Res Int 2021 1;2021:6657767. Epub 2021 Apr 1.

Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China.

Objectives: Oral squamous cell carcinoma (OSCC) is the most common oral cancer and has a poor prognosis. We aimed to identify new biomarkers or potential therapeutic targets for OSCC.

Materials And Methods: Four pairs of tumor and adjacent normal tissues were collected from OSCC patients, and differentially expressed genes (DEGs) were screened via high-throughput RNA sequencing (RNA-seq). Read More

View Article and Full-Text PDF

METTL3-Mediated N6-Methyladenosine Modification Is Involved in the Dysregulation of NRIP1 Expression in Down Syndrome.

Front Cell Dev Biol 2021 1;9:621374. Epub 2021 Apr 1.

Henan Provincial People's Hospital, Medical Genetics Institute of Henan Province, Henan Provincial Key Laboratory of Genetic Diseases and Functional Genomics, People's Hospital of Zhengzhou University, Zhengzhou, China.

Down syndrome (DS) is a common genetic condition in which a person is born with an extra copy of chromosome 21. Intellectual disability is the most common characteristic of DS. N6-methyladenosine (m6A) is a common RNA modification that is implicated in many biological processes. Read More

View Article and Full-Text PDF

RRM2B Is Frequently Amplified Across Multiple Tumor Types: Implications for DNA Repair, Cellular Survival, and Cancer Therapy.

Front Genet 2021 12;12:628758. Epub 2021 Mar 12.

Cancer Prevention and Control Program, Fox Chase Cancer Center, Philadelphia, PA, United States.

plays a crucial role in DNA replication, repair and oxidative stress. While germline mutations have been implicated in mitochondrial disorders, its relevance to cancer has not been established. Here, using TCGA studies, we investigated alterations in cancer. Read More

View Article and Full-Text PDF

General Control Non-derepressible 1 (AtGCN1) Is Important for Flowering Time, Plant Growth, Seed Development, and the Transcription/Translation of Specific Genes in .

Front Plant Sci 2021 31;12:630311. Epub 2021 Mar 31.

College of Life Sciences, Henan Agricultural University, Zhengzhou, China.

We have previously demonstrated that General Control Non-derepressible 1 (AtGCN1) is essential for translation inhibition under cold stress through interacting with GCN2 to phosphorylate eukaryotic translation initiation factor 2 (eIF2). Here, we report that the flower time of the mutant is later than that of the wild type (WT), and some siliques of cannot develop and produce seeds. Total and polysomal RNA of and wild type (WT) after cold treatments were sequenced. Read More

View Article and Full-Text PDF

RIM-Binding Proteins Are Required for Normal Sound-Encoding at Afferent Inner Hair Cell Synapses.

Front Mol Neurosci 2021 23;14:651935. Epub 2021 Mar 23.

Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany.

The afferent synapses between inner hair cells (IHC) and spiral ganglion neurons are specialized to faithfully encode sound with sub-millisecond precision over prolonged periods of time. Here, we studied the role of Rab3 interacting molecule-binding proteins (RIM-BP) 1 and 2 - multidomain proteins of the active zone known to directly interact with RIMs, Bassoon and Ca 1.3 - in IHC presynaptic function and hearing. Read More

View Article and Full-Text PDF

LncRNA OIP5-AS1 accelerates ox-LDL-treated HUVECs injury by NF-κB pathway via miR-30c-5p.

Clin Hemorheol Microcirc 2021 Apr 13. Epub 2021 Apr 13.

Department of Cardiology, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong, China.

Background: Oxidized low-density lipoprotein (ox-LDL) could induce endothelial injury and played a vital role in the progression and development of atherosclerosis. This study aimed to investigate the role of Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) in ox-LDL-induced human umbilical vascular endothelial cells (HUVECs) injury and the potential mechanisms.

Methods: Cell proliferation and apoptosis were evaluated by Cell Counting Kit-8 (CCK-8) assay and flow cytometry assay, respectively. Read More

View Article and Full-Text PDF

The impact of epigenetic information on genome evolution.

Philos Trans R Soc Lond B Biol Sci 2021 Jun 19;376(1826):20200114. Epub 2021 Apr 19.

School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Epigenetic information affects gene function by interacting with chromatin, while not changing the DNA sequence itself. However, it has become apparent that the interactions between epigenetic information and chromatin can, in fact, indirectly lead to DNA mutations and ultimately influence genome evolution. This review evaluates the ways in which epigenetic information affects genome sequence and evolution. Read More

View Article and Full-Text PDF

Cucumber Mosaic Virus 1a Protein Interacts with the Tobacco SHE1 Transcription Factor and Partitions between the Nucleus and the Tonoplast Membrane.

Plant Pathol J 2021 Apr 1;37(2):182-193. Epub 2021 Apr 1.

Department of Horticulture Sciences, Seoul Women's University, Seoul 01797, Korea.

The transcription factor SHE1 was identified as an interacting partner with the cucumber mosaic virus (CMV) 1a protein in the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nucleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but in the presence of the CMV 1a protein was partitioned between the nucleus and the tonoplast membrane. Read More

View Article and Full-Text PDF

In silico analysis and prediction of transcription factors of the proteins interacting with astrocyte elevated gene-1.

Comput Biol Chem 2021 Mar 29;92:107478. Epub 2021 Mar 29.

Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Kelambakkam, Chennai, 603 103, India. Electronic address:

Multifunctional in nature, the protein Astrocyte Elevated Gene-1 (AEG-1) controls several cancers through protein-protein interactions. Although, specific physiological processes and molecular functions linked with AEG-1 interactors remain unclear. In our present study, we procured the data of AEG-1 interacting proteins and evaluated their biological functions, associated pathways, and interaction networks using bioinformatic tools. Read More

View Article and Full-Text PDF

Structural basis of DEPTOR to recognize phosphatidic acid using its tandem DEP domains.

J Mol Biol 2021 Apr 15:166989. Epub 2021 Apr 15.

School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, the Sixth Affiliated Hospital, Guangzhou 510275, China. Electronic address:

DEP domain containing mTOR-interacting protein (DEPTOR) plays pivotal roles in regulating metabolism, growth, autophagy and apoptosis by functions as an endogenous inhibitor of mTOR signaling pathway. Activated by phosphatidic acid, a second messenger in mTOR signaling, DEPTOR dissociates from mTORC1 complex with unknown mechanism. Here, we present a 1. Read More

View Article and Full-Text PDF

Characterization of interactions between whey protein isolate and hyaluronic acid in aqueous solution: Effects of pH and mixing ratio.

Colloids Surf B Biointerfaces 2021 Apr 10;203:111758. Epub 2021 Apr 10.

Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun, Jilin, 130062, China. Electronic address:

Interactions between whey protein isolate (WPI) and hyaluronic acid (HA) were characterized as functions of pH (6.0-1.0) and protein to polysaccharide ratio (R, 1:4-10:1). Read More

View Article and Full-Text PDF

GENERALIZED AND TISSUE SPECIFIC GLUCOCORTICOID RESISTANCE.

Mol Cell Endocrinol 2021 Apr 14:111277. Epub 2021 Apr 14.

Department of Internal Medicine - Ribeirao Preto Medical School - University of Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address:

Glucocorticoids (GCs) are steroid hormones that influence several physiologic functions and are among the most frequently prescribed drugs worldwide. Resistance to GCs has been observed in the context of the familial generalized GC resistance (Chrousos' syndrome) or tissue specific GC resistance in chronic inflammatory states. In this review, we have summarized the major factors that influence individual glucocorticoid sensitivity/resistance. Read More

View Article and Full-Text PDF

Host PDZ-containing proteins targeted by SARS-Cov-2.

FEBS J 2021 Apr 17. Epub 2021 Apr 17.

Institut Pasteur, Unité Récepteurs-Canaux, UMR CNRS 3571, 75015, Paris, France.

Small linear motif targeting protein interacting domains called PDZ have been identified at the C-terminus of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins E, 3a, and N. Using a high-throughput approach of affinity-profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS-CoV-2 proteins E, 3A and N showing significant interactions with dissociation constants values ranging from 3 μM to 82 μM. Six of them (TJP1, PTPN13, HTRA1, PARD3, MLLT4, LNX2) are also recognized by SARS-CoV while three (NHERF1, MAST2, RADIL) are specific to SARS-CoV-2 E protein. Read More

View Article and Full-Text PDF

Methamphetamine exposure induces neuronal programmed necrosis by activating the receptor-interacting protein kinase 3 -related signalling pathway.

FASEB J 2021 May;35(5):e21561

School of Forensic Medicine, Southern Medical University, Guangzhou, P.R. China.

Methamphetamine (METH) is a synthetic drug with severe neurotoxicity, however, the regulation of METH-induced neuronal programmed necrosis remains poorly understood. The aim of this study was to identify the molecular mechanisms of METH-induced neuronal programmed necrosis. We found that neuronal programmed necrosis occurred in the striatum of brain samples from human and mice that were exposed to METH. Read More

View Article and Full-Text PDF

Expression of ISL1 and its partners in prostate cancer progression and neuroendocrine differentiation.

J Cancer Res Clin Oncol 2021 Apr 16. Epub 2021 Apr 16.

The Prostate Cancer Center, Calgary, AB, Canada.

Introduction And Objectives: ISL1 serves as a biomarker of metastasis and neuroendocrine neoplasia in multiple tumors. However, the expression and relation of ISL1 to other biomarkers in prostate cancer have not been fully elucidated. Here, we characterize the expression of ISL1 and its partners in PCa and document its association to disease progression and post castration resistance neuroendocrine differentiation. Read More

View Article and Full-Text PDF

ILDR2 stabilization is regulated by its interaction with GRP78.

Sci Rep 2021 Apr 16;11(1):8414. Epub 2021 Apr 16.

Division of Human Genetics, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.

Ildr2 was initially identified as a genetic modifier of diabetes susceptibility in B6.DBA Lep congenic mice, and was associated with decreased β-cell replication rates, reduced β-cell mass, and persistent mild hypoinsulinemic hyperglycemia. However, the molecular mechanisms of how the ILDR2 protein is involved in these effects are largely unknown. Read More

View Article and Full-Text PDF

Structural insight into the molecular mechanism of p53-mediated mitochondrial apoptosis.

Nat Commun 2021 04 16;12(1):2280. Epub 2021 Apr 16.

Department of Oncology, NHC Key Laboratory of Cancer Proteomics, Laboratory of Structural Biology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

The tumor suppressor p53 is mutated in approximately half of all human cancers. p53 can induce apoptosis through mitochondrial membrane permeabilization by interacting with and antagonizing the anti-apoptotic proteins BCL-xL and BCL-2. However, the mechanisms by which p53 induces mitochondrial apoptosis remain elusive. Read More

View Article and Full-Text PDF

The shared susceptibility epitope of HLA-DR4 binds citrullinated self-antigens and the TCR.

Sci Immunol 2021 Apr;6(58)

Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia.

Individuals expressing HLA-DR4 bearing the shared susceptibility epitope (SE) have an increased risk of developing rheumatoid arthritis (RA). Posttranslational modification of self-proteins via citrullination leads to the formation of neoantigens that can be presented by HLA-DR4 SE allomorphs. However, in T cell-mediated autoimmunity, the interplay between the HLA molecule, posttranslationally modified epitope(s), and the responding T cell repertoire remains unclear. Read More

View Article and Full-Text PDF

Down-regulation of pro-necroptotic molecules blunts necroptosis during myogenesis.

Biochem Biophys Res Commun 2021 Apr 13;557:33-39. Epub 2021 Apr 13.

Department of Applied Life Sciences, Graduate School, BK21 Program, Konkuk University, Chungju, 27478, Republic of Korea; Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju, 27487, Republic of Korea.

Cell death and differentiation are closely related at the molecular level. Differentiation of skeletal muscle cells attenuates susceptibility to apoptosis. Necroptosis has recently been recognized as a form of regulated cell death but its role in myogenesis has not been studied. Read More

View Article and Full-Text PDF

Mapping Interactions between Glycans and Glycan-Binding Proteins by Live Cell Proximity Tagging.

Curr Protoc 2021 Apr;1(4):e104

Department of Molecular Medicine, Scripps Research Institute, Jupiter, Florida.

Interactions between glycans and glycan-binding proteins (GBPs) consist of weak, noncovalent, and transient binding events, making them difficult to study in live cells void of a static, isolated system. Furthermore, the glycans are often presented as protein glycoconjugates, but there are limited efforts to identify these proteins. Proximity labeling permits covalent tagging of the glycoprotein interactors to query GBP in live cells. Read More

View Article and Full-Text PDF

Current Advances in Covalent Stabilization of Macromolecular Complexes for Structural Biology.

Bioconjug Chem 2021 Apr 16. Epub 2021 Apr 16.

Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Technical University of Munich, 85747 Garching, Germany.

Structural characterization of macromolecular assemblies is often limited by the transient nature of the interactions. The development of specific chemical tools to covalently tether interacting proteins to each other has played a major role in various fundamental discoveries in recent years. To this end, protein engineering techniques such as mutagenesis, incorporation of unnatural amino acids, and methods using synthetic substrate/cosubstrate derivatives were employed. Read More

View Article and Full-Text PDF