119,878 results match your criteria intact cells


Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.

Protein Cell 2021 Dec 5. Epub 2021 Dec 5.

State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

The use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. Read More

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December 2021

Novel carotenogenic gene combinations from red yeasts enhanced lycopene and beta-carotene production in Saccharomyces cerevisiae from the low-cost substrate sucrose.

FEMS Yeast Res 2021 Dec 4. Epub 2021 Dec 4.

National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.

Carotenoids (C40H56) including lycopene and beta-carotene are relatively strong antioxidants that provide benefits to human health. Here, we screened highly efficient crt variants from red yeasts to improve lycopene and beta-carotene production in Saccharomyces cerevisiae. We identified that crt variants from Sporidiobolus pararoseus TBRC-BCC 63403 isolated from rice leaf in Thailand exhibited the highest activity in term of lycopene and beta-carotene production in the context of yeast. Read More

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December 2021

Genes for tRNA recycling are upregulated in response to infection with Theiler's mouse encephalitis virus.

Biochem Biophys Res Commun 2021 Nov 30;587:63-68. Epub 2021 Nov 30.

Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Niigata, 956-8603, Japan.

The concept of tRNA recycling has recently emerged from the studies of ribosome-associated quality control. Therein tRNase Z removes the 2', 3'>p from the ANKZF1-cleaved tRNA and the subsequent TRNT1 action re-generates the intact tRNA. To know the roles of the tRNA recycling in vivo, we investigated how viral infection affects the tRNA recycling system by analyzing the mRNA levels of tRNase Z and TRNT1. Read More

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November 2021

Bioelectricity generation from live marine photosynthetic macroalgae.

Biosens Bioelectron 2021 Nov 27;198:113824. Epub 2021 Nov 27.

Grand Technion Energy Program, Technion, Haifa, 32000, Israel; Schulich Faculty of Chemistry, Technion, Haifa, 320000, Israel. Electronic address:

The conversion of solar energy into electrical current by photosynthetic organisms has the potential to produce clean energy. Bio-photoelectrochemical cells (BPECs) utilizing unicellular photosynthetic microorganisms have been studied, however similar harvesting of electrons from more evolved intact photosynthetic organisms has not been previously reported. In this study, we describe for the first time BPECs containing intact live marine macroalgae (seaweeds) in natural seawater or saline buffer. Read More

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November 2021

Dual-modified nanoparticles overcome sequential absorption barriers for oral insulin delivery.

J Control Release 2021 Dec 2. Epub 2021 Dec 2.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China; NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients, National Institutes for Food and Drug Control, Beijing 100050, China. Electronic address:

The efficacy of oral insulin drug delivery is seriously hampered by multiple gastrointestinal barriers, especially transepithelial barriers, including apical endocytosis, lysosomal degradation, cytosolic diffusion and basolateral exocytosis. In this study, a functional nanoparticle (PG-FAPEP) with dual-modification was constructed to sequentially address these important absorption obstacles for improved oral insulin delivery. The dual surface decorations folate and charge-convertible tripeptide endowed PG-FAPEP with the ability to target the apical and basolateral sides of enterocytes, respectively. Read More

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December 2021

Theoretical study of Myriocin binding mechanism targeting Serine Palmitoyltransferase.

Chem Biol Drug Des 2021 Dec 3. Epub 2021 Dec 3.

School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Sphingolipids (SLs) are vital for cells as forming membrane and transducing signals. The first step for de novo biosynthesis of SLs is catalyzed by the pyridoxal-5'-phosphate (PLP)-dependent enzyme Serine palmitoyltransferase (SPT), which has been proven to be a promising drug target for treating various diseases. However, there are few SPT-specific inhibitors have been identified so far. Read More

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December 2021

Metaphase-Based Cytogenetic Approach Identifies Radiation-Induced Chromosome and Chromatid Aberrations in Zebrafish Embryos.

Radiat Res 2021 Dec 3. Epub 2021 Dec 3.

Singapore Nuclear Research and Safety Initiative, National University of Singapore, Singapore.

Metaphase-based cytogenetic methods based on scoring of chromosome aberrations for the estimation of the radiation dose received provide a powerful approach for evaluating the associated risk upon radiation exposure and form the bulk of our current knowledge of radiation-induced chromosome damages. They mainly rely on inducing quiescent peripheral lymphocytes into proliferation and blocking them at metaphases to quantify the damages at the chromosome level. However, human organs and tissues demonstrate various sensitivity towards radiation and within them, self-proliferating progenitor/stem cells are believed to be the most sensitive populations. Read More

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December 2021

Answers and Questions: Forty Years in Bioelectromagnetics.

Authors:
Ben Greenebaum

Bioelectromagnetics 2021 Dec 2. Epub 2021 Dec 2.

Physics Department, University of Wisconsin-Parkside, Kenosha, Wisconsin.

The work began in 1972 when three young assistant professors used a slime mold to see if electromagnetic fields would affect it. The fields did, though the effects were small and hard to tease out of the noise. The cell cycle was lengthened and there were changes in respiration. Read More

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December 2021

Intact Preparation of the Lymph Gland for a Comprehensive Analysis of Larval Hematopoiesis.

Bio Protoc 2021 Nov 5;11(21):e4204. Epub 2021 Nov 5.

Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.

Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver, and spleen, rendering a comprehensive analysis of the entire HSPC pool nearly impossible. The blood system is well studied and has developmental, molecular, and functional parallels with that of vertebrates. Read More

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November 2021

Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells.

Front Cell Dev Biol 2021 11;9:728383. Epub 2021 Nov 11.

Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Actomyosin contractility regulates various cellular processes including proliferation and differentiation while dysregulation of actomyosin activity contributes to cancer development and progression. Previously, we have reported that actomyosin-generated tension at adherens junctions is required for cell density-dependent inhibition of proliferation of normal skin keratinocytes. However, it remains unclear how actomyosin contractility affects the hyperproliferation ability of cutaneous squamous cell carcinoma (cSCC) cells. Read More

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November 2021

Key Role of Staphylococcal Fibronectin-Binding Proteins During the Initial Stage of Keratitis in Humans.

Front Cell Infect Microbiol 2021 9;11:745659. Epub 2021 Nov 9.

CIRI, Centre International de Recherche en Infectiologie, GIMAP Team, University of Lyon, University of St-Etienne, INSERM U1111, CNRS UMR5308, ENS de Lyon, UCBL1, St-Etienne, France.

Objectives: is one of the main causes of bacterial keratitis in humans. This study was aimed at investigating the mechanisms of adhesion to the human corneal epithelium involved during the initial stage of infectious keratitis.

Methods: Human corneas stored in a specific active storage machine that restores a normal pluristratified epithelium were used to assess adhesion level to intact and injured tissues using immunostaining. Read More

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November 2021

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors.

Nat Commun 2021 Dec 2;12(1):7019. Epub 2021 Dec 2.

Stem Cell Biology Group, Cancer Research UK Manchester Institute, The University of Manchester, Macclesfield, SK10 4TG, UK.

Yolk sac (YS) hematopoiesis is critical for the survival of the embryo and a major source of tissue-resident macrophages that persist into adulthood. Yet, the transcriptional and epigenetic regulation of YS hematopoiesis remains poorly characterized. Here we report that the epigenetic regulator Ezh2 is essential for YS hematopoiesis but dispensable for subsequent aorta-gonad-mesonephros (AGM) blood development. Read More

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December 2021

Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks.

Nat Commun 2021 Dec 2;12(1):7036. Epub 2021 Dec 2.

Department of Health Sciences and Technology (D-HEST), ETH Zurich, Institute of Translational Medicine (ITM), Zurich, Switzerland.

The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). Read More

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December 2021

DropletQC: improved identification of empty droplets and damaged cells in single-cell RNA-seq data.

Genome Biol 2021 Dec 2;22(1):329. Epub 2021 Dec 2.

Garvan Weizmann Centre for Cellular Genomics, Garvan Institute of Medical Research, The Kinghorn Cancer Centre, Darlinghurst, NSW, 2010, Australia.

Background: Advances in droplet-based single-cell RNA-sequencing (scRNA-seq) have dramatically increased throughput, allowing tens of thousands of cells to be routinely sequenced in a single experiment. In addition to cells, droplets capture cell-free "ambient" RNA predominantly caused by lysis of cells during sample preparation. Samples with high ambient RNA concentration can create challenges in accurately distinguishing cell-containing droplets and droplets containing ambient RNA. Read More

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December 2021

Development of a mouse model of ascending infection and preterm birth.

PLoS One 2021 2;16(12):e0260370. Epub 2021 Dec 2.

Division of Maternal-Fetal Medicine and Perinatal Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America.

Background: Microbial invasion of the intraamniotic cavity and intraamniotic inflammation are factors associated with spontaneous preterm birth. Understanding the route and kinetics of infection, sites of colonization, and mechanisms of host inflammatory response is critical to reducing preterm birth risk.

Objectives: This study developed an animal model of ascending infection and preterm birth with live bacteria (E. Read More

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December 2021

Advantages of the Liposomal Form of Xymedon in Leukopoiesis Restoration against the Background of Myelosuppressive Therapy with Liposomal Antineoplastic Drugs in Experiment.

Bull Exp Biol Med 2021 Dec 2;172(2):195-201. Epub 2021 Dec 2.

N. P. Ogarev National Research Mordovia State University, Saransk, Republic of Mordovia, Russia.

We analyzed advantages of the liposomal form of Xymedon (50 and 100 mg/kg) over free Xymedon (in the corresponding doses) in leukopoiesis restoration in rats with Walker-256 carcinoma treated with liposomal combination of doxorubicin (4 mg/kg) and cyclophosphamide (45 mg/kg) (single intravenous injection on day 11 after transplantation of tumor cells). Liposomal and free Xymedon were injected intravenously over 5 days starting from day 11 of the experiment. Changes in leukopoiesis in peripheral blood and myelograms were assessed on days 3 and 7 after chemotherapy. Read More

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December 2021

Crosstalk between protein kinases A and C regulates sea urchin sperm motility.

Zygote 2021 Dec 2:1-12. Epub 2021 Dec 2.

Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Apdo. Postal 510-3, Cuernavaca, Morelos, México62210.

Fertilization, a crucial event for species preservation, in sea urchins, as in many other organisms, requires sperm motility regulation. In Strongylocentrotus purpuratus sea urchins, speract, a sperm chemoattractant component released to seawater from the outer egg layer, attracts sperm after binding to its receptor in the sperm flagellum. Previous experiments performed in demembranated sperm indicated that motility regulation in these cells involved protein phosphorylation mainly due to the cAMP-dependent protein kinase (PKA). Read More

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December 2021

VgrG-dependent effectors and chaperones modulate the assembly of the type VI secretion system.

PLoS Pathog 2021 Dec 1;17(12):e1010116. Epub 2021 Dec 1.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

The type VI secretion system (T6SS) is a spear-like nanomachine found in gram-negative pathogens for delivery of toxic effectors to neighboring bacterial and host cells. Its assembly requires a tip spike complex consisting of a VgrG-trimer, a PAAR protein, and the interacting effectors. However, how the spike controls T6SS assembly remains elusive. Read More

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December 2021

Direct evaluation of cohesin-mediated sister kinetochore associations at meiosis I in fission yeast.

J Cell Sci 2021 Dec 1. Epub 2021 Dec 1.

Faculty of Science, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.

Kinetochores drive chromosome segregation by mediating chromosome interactions with the spindle. In higher eukaryotes, sister kinetochores are separately positioned on opposite sides of sister centromeres during mitosis, but associate with each other during meiosis I. Kinetochore association facilitates the attachment of sister chromatids to the same pole, enabling the segregation of homologous chromosomes toward opposite poles. Read More

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December 2021