153,667 results match your criteria injury rat

Integrated analysis of long noncoding RNAs and mRNA expression profiles reveals the potential role of lncRNAs in early stage of post-peripheral nerve injury in Sprague-Dawley rats.

Aging (Albany NY) 2021 May 8;13. Epub 2021 May 8.

Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.

The regulatory role of lncRNAs in the early stage post peripheral nerve injury (PNI) is not yet clear. In this study, next-generation sequencing was used to perform deep sequencing on normal sciatic nerves (control) and lesional tissues derived on the 4th (D4) and 7th days (D7) after sciatic nerve injury in rats. Time-point unique differentially expressed lncRNAs (DElncRNAs) were analyzed for functional enrichment. Read More

View Article and Full-Text PDF

Synergistic cardioptotection by tilianin and syringin in diabetic cardiomyopathy involves interaction of TLR4/NF-κB/NLRP3 and PGC1a/SIRT3 pathways.

Int Immunopharmacol 2021 May 7;96:107728. Epub 2021 May 7.

Department of Endocrinology and Metabolism, Changzhou No.2 People's Hospital, Changzhou, Jiangsu 213003, China. Electronic address:

Diabetic cardiomyopathy (DCM) is a chronic multifactorial complication of type-2 diabetes mellitus, leading to heart failure. A combination of multifaceted therapeutics for the management of DCM is needed. Here, we investigated the combined effect of syringin and tilianin on DCM by evaluating cardiac function, inflammation, oxidative stress, apoptosis and mitochondrial function, and explored the contribution of TLR4/NF-κB/NLRP3 and PGC1α/SIRT3 pathways in diabetic rats and hyperglycemic-H9c2 cells. Read More

View Article and Full-Text PDF

JTE-013 Alleviates Inflammatory Injury and Endothelial Dysfunction Induced by Sepsis In Vivo and Vitro.

J Surg Res 2021 May 7;265:323-332. Epub 2021 May 7.

Special Functions Section, The Fourth Sanatorium Area of Hangzhou Special Service Sanatorium Center of Air Force, Nanjing City, Jiangsu Province, China. Electronic address:

Background: Nowadays, there is no approved targeted agent for lung injury induced by sepsis. S1PR2 is confirmed to be a promising diagnosis and treatment target. JTE-013 as S1PR2 antagonists may be an agent of great potential. Read More

View Article and Full-Text PDF

Macrophage activation in the lung during the progression of nitrogen mustard induced injury is associated with histone modifications and altered miRNA expression.

Toxicol Appl Pharmacol 2021 May 7:115569. Epub 2021 May 7.

Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, 08854, USA; Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, NJ 08854, USA. Electronic address:

Activated macrophages have been implicated in lung injury and fibrosis induced by the cytotoxic alkylating agent, nitrogen mustard (NM). Herein, we determined if macrophage activation is associated with histone modifications and altered miRNA expression. Treatment of rats with NM (0. Read More

View Article and Full-Text PDF

PU.1 Interaction with p50 Promotes Microglial-mediated Inflammation in Secondary Spinal Cord Injury in SCI Rats.

Int J Neurosci 2021 May 10:1-15. Epub 2021 May 10.

Department of Orthopedics, Changzhou Seventh People's Hospital, Changzhou, Jiangsu Province 213000, China.

Secondary spinal cord injury is the inflammatory damage to surrounding tissues caused by activated microglial-mediated neuroinflammatory responses. The nuclear factor-κB (p65/p50) pathway and PU.1 are closely correlated with inflammatory responses; thus, we examined the relationship and function between PU. Read More

View Article and Full-Text PDF

Withania coagulans extract attenuates oxidative stress-mediated apoptosis of cerebellar purkinje neurons after ischemia/reperfusion injury.

Metab Brain Dis 2021 May 10. Epub 2021 May 10.

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, IR, Iran.

Cerebral ischemia/reperfusion (I/R) is known to increase reactive oxygen species (ROS) generation, consequences of oxidative stress (OS), and neuronal death in the susceptible brain areas including the cerebellum. Newly, remarkable attention has been paid to a natural diet with the capability to scavenge ROS. Withania coagulans root extract (WCE) is rich in components with antioxidants properties. Read More

View Article and Full-Text PDF

Multiple Particle Tracking Detects Changes in Brain Extracellular Matrix and Predicts Neurodevelopmental Age.

ACS Nano 2021 May 10. Epub 2021 May 10.

Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, United States.

Brain extracellular matrix (ECM) structure mediates many aspects of neural development and function. Probing structural changes in brain ECM could thus provide insights into mechanisms of neurodevelopment, the loss of neural function in response to injury, and the detrimental effects of pathological aging and neurological disease. We demonstrate the ability to probe changes in brain ECM microstructure using multiple particle tracking (MPT). Read More

View Article and Full-Text PDF

Frequency selective neuronal modulation triggers spreading depolarizations in the rat endothelin-1 model of stroke.

J Cereb Blood Flow Metab 2021 May 9:271678X211013656. Epub 2021 May 9.

Sunnybrook Research Institute, Physical Sciences, Toronto, ON, Canada.

Ischemia is one of the most common causes of acquired brain injury. Central to its noxious sequelae are spreading depolarizations (SDs), waves of persistent depolarizations which start at the location of the flow obstruction and expand outwards leading to excitotoxic damage. The majority of acute stage of stroke studies to date have focused on the phenomenology of SDs and their association with brain damage. Read More

View Article and Full-Text PDF

Protective effect of aldehyde dehydrogenase 2 against rat corneal dysfunction caused by streptozotocin-induced type I diabetes.

Exp Biol Med (Maywood) 2021 May 8:15353702211013308. Epub 2021 May 8.

Traditional Chinese Medicine (TCM) Department, General Hospital of Western Theater Command, Chengdu 610083, PR China.

Aldehyde dehydrogenase 2 plays a pivotal role in detoxifying aldehydes, and our previous study revealed that aldehyde dehydrogenase 2 could alleviate diabetic retinopathy-associated damage. We aimed to characterize the potential role of aldehyde dehydrogenase 2 in diabetic keratopathy. Twenty-four rats with streptozotocin-induced (60 mg/kg, single intraperitoneal injection) type 1 diabetes mellitus (T1DM) were divided the T1DM group and the T1DM + Alda1 (an activator of aldehyde dehydrogenase 2) group (5 mg/kg/d, intraperitoneal injection, 1/2/3 months), while an additional 12 healthy rats served as the control group. Read More

View Article and Full-Text PDF

Pre-existing hypertension and pregnancy induced hypertension reveal molecular differences in placental proteome in rodents.

Physiol Genomics 2021 May 10. Epub 2021 May 10.

BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Scotlandgrid.8756.c.

Pre-existing or new onset of hypertension affects pregnancy and is one of the leading causes of maternal and fetal morbidity and mortality. In certain cases, it also leads to long term maternal cardiovascular complications. The placenta is a key player in the pathogenesis of complicated hypertensive pregnancies, however the pathomechanisms leading to an abnormal placenta are poorly understood. Read More

View Article and Full-Text PDF

MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7.

Folia Neuropathol 2021 ;59(1):67-80

Department of Neurology, The First Affiliated Hospital of Baotou Medical College, China.

Introduction: MicroRNA (miRNA) is known to be involved in nerve injury. Our study aimed to identify the role and mechanism of miR-1b in rat neuron proliferation, regeneration and apoptosis.

Material And Methods: Neurons were successfully separated and identified using a microscope and immunofluorescence staining of microtubule-associated protein 2 (MAP-2). Read More

View Article and Full-Text PDF
January 2021

Bone marrow stromal cells reverse the microglia type from pro-inflammatory tumour necrosis factor a microglia to anti-inflammatory CD206 microglia of middle cerebral artery occlusion rats through triggering secretion of CX3CL1.

Folia Neuropathol 2021 ;59(1):20-31

Geriatric Neurological Department of the Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese People's Liberation Army General Hospital, Beijing, China.

The middle cerebral artery occlusion (MCAO) model has been extensively applied to study ischaemic stroke. This study attempted to clarify effect of bone marrow stromal cells (BMSCs) on infarct injury of MCAO rats. BMSCs were isolated and identified by staining CD29/CD44 and CD31/CD45. Read More

View Article and Full-Text PDF
January 2021

Acetovanillone prevents cyclophosphamide-induced acute lung injury by modulating PI3K/Akt/mTOR and Nrf2 signaling in rats.

Phytother Res 2021 May 9. Epub 2021 May 9.

Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt.

Cyclophosphamide (CP) is a medication used as an anticancer drug and to suppress the immune system. However, its clinical applications are restricted because of the toxic and adverse side effects. The present study investigated the protective effect of acetovanillone (AV), a natural NADPH oxidase inhibitor, against acute lung injury (ALI) induced by CP. Read More

View Article and Full-Text PDF

Effects of omega 3 polyunsaturated fatty acids, antioxidants and/or non-steroidal inflammatory drugs in the brain of neonatal rats exposed to intermittent hypoxia.

Int J Dev Neurosci 2021 May 9. Epub 2021 May 9.

Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, 11203, USA.

Preterm infants experience frequent arterial oxygen desaturations during oxygen therapy, or intermittent hypoxia (IH). Neonatal IH increases oxidative distress which contributes to neuroinflammation and brain injury. We tested the hypotheses that exposure to neonatal IH is detrimental to the immature brain, and that early supplementation with antioxidants and/or omega 3 polyunsaturated fatty acids (n-3 PUFAs) combined with non-steroidal anti-inflammatory drugs (NSAIDs) is protective. Read More

View Article and Full-Text PDF

Remifentanil preconditioning alleviates myocardial ischemia/reperfusion injury in rats via activating Jagged-1/Notch signaling pathway.

Biosci Rep 2021 May 10. Epub 2021 May 10.

Chengdu Second People's Hospital, Chengdu, China.

Ischemic heart diseases have emerged as great threats to human health. Nowadays, restoration of cardiac blood flow supply is widely regarded as a feasible treatment choice for ischemic heart diseases; however, this intervention would contradictorily elicit reperfusion injury. Recently, myocardial ischemia/reperfusion injury (MI/RI) has aroused widespread public concerns. Read More

View Article and Full-Text PDF

The interplay between HIF-1α and long noncoding GAS5 regulates the JAK1/STAT3 signalling pathway in hypoxia-induced injury in myocardial cells.

Cardiovasc Diagn Ther 2021 Apr;11(2):422-434

Liaoning Jinzhou Inspection and Testing Certification Center, Jinzhou, China.

Background: Long non-coding RNA (lncRNA) GAS5 is associated with hypoxia-induced diseases whereas hypoxia-inducible factor-1α (HIF-1α) plays an important role in hypoxic injury of cells. The current study explores the regulatory functions of GAS5/HIF-1α which co-play in anoxic injury among rat cardiomyocytes H9C2 cells.

Methods: Hypoxia model was established through anoxic incubation while normal culture of H9C2 cells was considered as control. Read More

View Article and Full-Text PDF

Plasma Small Extracellular Vesicle-Carried miRNA-501-5p Promotes Vascular Smooth Muscle Cell Phenotypic Modulation-Mediated In-Stent Restenosis.

Oxid Med Cell Longev 2021 21;2021:6644970. Epub 2021 Apr 21.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Vascular smooth muscle cell (VSMC) phenotypic modulation plays an important role in the occurrence and development of in-stent restenosis (ISR), the underlying mechanism of which remains a key issue needing to be urgently addressed. This study is designed to investigate the role of plasma small extracellular vesicles (sEV) in VSMC phenotypic modulation. sEV were isolated from the plasma of patients with ISR (ISR-sEV) or not (Ctl-sEV) 1 year after coronary stent implantation using differential ultracentrifugation. Read More

View Article and Full-Text PDF

Fisetin Alleviates Neointimal Hyperplasia via PPAR/PON2 Antioxidative Pathway in SHR Rat Artery Injury Model.

Oxid Med Cell Longev 2021 21;2021:6625517. Epub 2021 Apr 21.

Medical and Healthcare Center, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, China.

The phenotypic transformation of proliferation and migration in vascular smooth muscle cells (VSMCs) from media to intima is the basic pathology of neointimal hyperplasia after angioplasty in hypertensive patients. Angiotensin II (AngII) stimulates oxidative stress in VSMC, inducing VSMC proliferation and migration, which is a critical factor in both developments of hypertension and angioplasty-induced arterial restenosis. Fisetin, a plant flavonoid polyphenol, has been reported to be antioxidative and potent senolytic. Read More

View Article and Full-Text PDF

Ghrelin Alleviates Intestinal Dysfunction in Sepsis Through the KLF4/MMP2 Regulatory Axis by Activating SIRT1.

Front Immunol 2021 23;12:646775. Epub 2021 Apr 23.

Department of Respiratory, The First Hospital of Lanzhou University (The First School of Clinical Medicine), Lanzhou, China.

Intestinal barrier dysfunction is an important contributor to morbidity caused by sepsis. This study investigates the molecular mechanism by which Ghrelin affects intestinal dysfunction in rat model of sepsis. A rat model of sepsis was established by cecal ligation and puncture (CLP), revealing that Ghrelin was downregulated when sepsis occurs. Read More

View Article and Full-Text PDF

Pro-Resolving Mediator Annexin A1 Regulates Intracellular Ca and Mucin Secretion in Cultured Goblet Cells Suggesting a New Use in Inflammatory Conjunctival Diseases.

Front Immunol 2021 22;12:618653. Epub 2021 Apr 22.

Schepens Eye Research institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, United States.

The amount of mucin secreted by conjunctival goblet cells is regulated to ensure the optimal level for protection of the ocular surface. Under physiological conditions lipid specialized pro-resolving mediators (SPM) are essential for maintaining tissue homeostasis including the conjunctiva. The protein Annexin A1 (AnxA1) can act as an SPM. Read More

View Article and Full-Text PDF

Transforming Growth Factor-β3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats.

Front Pharmacol 2021 23;12:678322. Epub 2021 Apr 23.

Institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China.

Patients with a skull defect are at risk of developing cerebrospinal fluid leakage and ascending bacterial meningitis at >10% per year. However, treatment with stem cells has brought great hope to large-area cranial defects. Having found that transforming growth factor (TGF)-β3 can promote the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), we designed a hybrid TGF-β3/recombinant human-like collagen recombinant human collagen/chitosan (CS) freeze-dried sponge (TRFS) loading hPDLSCs (TRFS-h) to repair skull defects in rats. Read More

View Article and Full-Text PDF

Yi-Qi-Jian-Pi Formula Suppresses RIPK1/RIPK3-Complex-Dependent Necroptosis of Hepatocytes Through ROS Signaling and Attenuates Liver Injury and .

Front Pharmacol 2021 23;12:658811. Epub 2021 Apr 23.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.

Acute-on-chronic liver failure (ACLF) is described as a characteristic of acute jaundice and coagulation dysfunction. Effective treatments for ACLF are unavailable and hence are urgently required. We aimed to define the effect of Yi-Qi-Jian-Pi Formula (YQJPF) on liver injury and further examine the molecular mechanisms. Read More

View Article and Full-Text PDF

Emodin Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury by Inhibiting the Cold-Inducible RNA-Binding Protein (CIRP)-Mediated Activation of the NLRP3/IL-1/CXCL1 Signaling.

Front Pharmacol 2021 23;12:655372. Epub 2021 Apr 23.

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

Severe acute pancreatitis (SAP) can lead to acute lung injury (ALI). This study investigated the therapeutic effect of emodin and its molecular mechanisms in a rat model of SAP-ALI. Forty male Sprague-Dawley rats were randomly divided into the groups: Control (CON), SAP (SAP), emodin (EMO), and C23 (C23). Read More

View Article and Full-Text PDF

Pathogenic Functions of Tumor Necrosis Factor Receptor- Factor 6 Signaling Traumatic Brain Injury.

Front Mol Neurosci 2021 21;14:629910. Epub 2021 Apr 21.

Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong, China.

Neuroinflammation contributes to delayed (secondary) neurodegeneration following traumatic brain injury (TBI). Tumor necrosis factor receptor-associated factor 6 (TRAF6) signaling may promote post-TBI neuroinflammation, thereby exacerbating secondary injury. This study investigated the pathogenic functions of TRAF6 signaling following TBI and . Read More

View Article and Full-Text PDF

Synaptic changes and the response of microglia in a light-induced photoreceptor degeneration model.

Mol Vis 2021 1;27:206-220. Epub 2021 May 1.

Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Purpose: To explore synaptic changes and the response of microglia in a light-induced photoreceptor degeneration model.

Methods: Sprague-Dawley rats were euthanized 1 h, 1 day, 3 days, 7 days, and 14 days after being exposed to intense blue light for 24 h. Hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used to evaluate changes in the outer nuclear layer (ONL). Read More

View Article and Full-Text PDF

Enterococcus faecalis contributes to hypertension and renal injury in Sprague-Dawley rats by disturbing lipid metabolism.

J Hypertens 2021 Jun;39(6):1112-1124

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine.

Objective: Increasing studies have demonstrated that gut microbiota play vital roles in the development of hypertension. However, the underlying mechanism is not fully understood.

Methods: The relative abundance of Enterococcus faecalis was determined in the faecal samples of angiotensin II or deoxycorticosterone acetate/salt-induced hypertensive rats. Read More

View Article and Full-Text PDF

Animal models for study on rotator cuff injury.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):426-431

Department of Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Rotator cuff injuries are the most common cause of shoulder pain and dysfunction. Ideal animal shoulder models should have similar shoulder anatomy and function as human, and are able to replicate the microenvironment change after tendon injury. At present, a variety of animal models including rat, mouse, rabbit, sheep, canine, bovine, and primate have been used to study the mechanism of rotator cuff injury, effects of different repair techniques, and factors affecting tendon to bone healing. Read More

View Article and Full-Text PDF

Transcriptome analysis of mild hypothermia protection against radiationinduced rat lung injury based on RNAseq.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):345-350

Drug Safety Evaluation Center of China Institute for Radiation Protection; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury; Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China.

Objectives: To analyze the differentially expressed genes (DEGs) with radiation-induced rat lung injury, and to reveal the protective mechanism for mild hypothermia in the radiation-induced lung injury in rats at the transcriptome level.

Methods: A total of 10 male SD rats aged 6-8 weeks were randomly divided into 2 groups to establish a rat model of radiation-induced lung injury, and one group was treated with mild hypothermia. RNA was extracted from left lung tissue of each group, and sequenced by BGISEQ-500 platform. Read More

View Article and Full-Text PDF

The Molecular Mechanism of Scutellaria baicalensis Georgi Stems and Leaves Flavonoids in Promoting Neurogenesis and Improving Memory Impairment by the PI3K-AKT-CREB Signaling Pathway in Rats.

Comb Chem High Throughput Screen 2021 May 6. Epub 2021 May 6.

The Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, China.

Aim: The aim of this study was to investigate the effect, and molecular mechanism of Scutellaria Baicalensis Georgi stems and leaves flavonoids (SSF) in promoting neurogenesis and improving memory impairment induced by the PI3K-AKT-CREB signaling pathway.

Methods: Alzheimer's disease (AD) was induced in the male Wistar rats by intracerebroventricular injection of amyloid beta-peptide 25-35 (Aβ25-35) in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1(RHTGF-β1) (composited Aβ). The Morris water maze was used to screen the successful establishment of the memory impairment model of rats. Read More

View Article and Full-Text PDF

The protective effects of topiramate on intestinal injury induced with infrarenal aortic occlusion via oxidative stress and apoptosis.

Clin Exp Hypertens 2021 May 10:1-6. Epub 2021 May 10.

Department of General Surgery, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.

: Prolonged surgical procedures and some clinical conditions such as surgeries of thoracoabdominal aorta, mesenteric ischemia, cardiopulmonary bypass, strangulated hernias and neonatal necrotizing enterocolitis may cause decreased perfusion and injury of relevant organs and tissues. After reperfusion, injuries may get worse, leading to ischemia-reperfusion (I/R) injury. Reperfusion following arterial clamping allows oxygen to ischemic tissues and produce injury by multiple mechanisms, including neutrophilic infiltration, intracellular adhesion molecules, and generation of reactive oxygen radicals. Read More

View Article and Full-Text PDF