991,236 results match your criteria inhibition


Islet Gene View-a tool to facilitate islet research.

Life Sci Alliance 2022 Dec 10;5(12). Epub 2022 Aug 10.

Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden

Characterization of gene expression in pancreatic islets and its alteration in type 2 diabetes (T2D) are vital in understanding islet function and T2D pathogenesis. We leveraged RNA sequencing and genome-wide genotyping in islets from 188 donors to create the Islet Gene View (IGW) platform to make this information easily accessible to the scientific community. Expression data were related to islet phenotypes, diabetes status, other islet-expressed genes, islet hormone-encoding genes and for expression in insulin target tissues. Read More

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December 2022

Stability of hen egg-white lysozyme during embryonic development.

Biosci Biotechnol Biochem 2022 Aug 10. Epub 2022 Aug 10.

Faculty of Food and Nutrition, Kyushu Nutrition and Welfare University, Kokura-Kitaku, Kitakyushu 803-8511, Japan.

It is of interest to determine whether and how egg-white proteins are maintained in fertile eggs. We previously observed that egg-white ovalbumin attained high stability during embryogenesis. Herein, we observed that the total mass of egg white and that of its gross protein content showed a decrease according to the days of incubation. Read More

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Radiation combined with KRAS-MEK inhibitors enhances anticancer immunity in KRAS-mutated tumor models.

Transl Res 2022 Aug 7. Epub 2022 Aug 7.

Department of Radiation Oncology, Shandong Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan 250117, Shandong, P. R. China; Department of Oncology, Shandong Cancer Hospital and Institute, Shandong University, Jinan 250021, Shandong, P. R. China. Electronic address:

KRAS mutation is a common driver in solid tumors, and KRAS-mutated tumors are relatively resistant to radiotherapy. Therefore, we investigated the combined effect of radiation and KRAS-MEK inhibitors (AMG510 and trametinib) in KRAS-mutated tumors. The expression of programmed death-ligand 1 (PD-L1), major histocompatibility complex (MHC) class I molecules, and cytokines in KRAS-mutated cell lines was assessed using flow cytometry, western blot analysis, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay. Read More

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Single-injection COVID-19 subunit vaccine elicits potent immune responses.

Acta Biomater 2022 Aug 7. Epub 2022 Aug 7.

Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China; School of Chemistry, Tiangong University, Tianjin 300387, China. Electronic address:

Current vaccination schedules, including COVID-19 vaccines, require multiple doses to be administered. Single injection vaccines eliciting equivalent immune response are highly desirable. Unfortunately because unconventional release kinetics are difficult to achieve it still remains a huge challenge. Read More

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Pharmacological evidence that potassium channels mediate hydrogen sulfide-induced inhibition of the vasopressor sympathetic outflow in pithed rats.

Eur J Pharmacol 2022 Aug 7:175160. Epub 2022 Aug 7.

Departamento de Farmacobiología, Cinvestav-Coapa, Czda. de Los Tenorios 235, Col. Granjas-Coapa, Del, Tlalpan, C.P. 14330, México D.F, Mexico. Electronic address:

Hydrogen sulfide (HS) is a gasotransmitter that modulates neurotransmission. Indeed, it has been recently demonstrated that HS inhibits the sympathetic outflow in male rats, although the mechanisms remain elusive. Thus, this study evaluated the role of potassium channels on NaHS-induced sympathoinhibition. Read More

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Anti-inflammatory withajardins from the leaves of Athenaea velutina.

Phytochemistry 2022 Aug 7:113338. Epub 2022 Aug 7.

Departamento de Química Orgânica e Inorgânica, Centro de Ciências,Universidade Federal Do Ceará, 60021-970, Fortaleza, CE, Brazil. Electronic address:

Withajardins, uncommon modified withanolide-type steroids, have been isolated exclusively from plants of the Solanaceae family so far. Two undescribed withajardins and the known tuboanosigenin were isolated from the hexane/EtOAc 1:1 extract from Athenaea velutina leaves. Their structures were established by an extensive analysis of 1D and 2D-NMR and HRMS data. Read More

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Recent advances in natural products as potential inhibitors of dengue virus with a special emphasis on NS2b/NS3 protease.

Phytochemistry 2022 Aug 7:113362. Epub 2022 Aug 7.

Pharmaceutical Design and Simulation (PhDS) Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia; Discipline of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia. Electronic address:

Dengue virus (DENV) is an arbovirus widespread through tropical and subtropical areas. It is transmitted to humans through Aedes mosquitoes. Infections with DENV can lead to a series of complications, including dengue fever, dengue haemorrhagic fever, or dengue shock syndrome, which might manifest through secondary infections because of a vulnerable immune system. Read More

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Diagnosis of drug-induced liver injury in model mice by studying the inhibitory effect of serum components on P450 inhibition assay.

Chem Biol Interact 2022 Aug 7:110075. Epub 2022 Aug 7.

Laboratory of Response to Environmental Materials, Division of Signal Responses, Biosignal Research Center, Kobe University, Japan. Electronic address:

Drug-induced liver injury (DILI) causes abortive drug development in both clinical and non-clinical phases. Therefore, it is important to develop an evaluation system that can be used to accurately and easily assess DILI during drug development and at an early stage. The diagnosis of diseases using P450 inhibition assays, with focus on changes in cytochrome P450 (CYP, P450) during disease onset, has been previously validated in mouse models of ulcerative colitis and type 2 diabetes. Read More

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Nitrogen-dependent luteolin effect on Microcystis growth and microcystin-pollution risk - Novel mechanism insights unveiled by comparative proteomics and gene expression.

Environ Pollut 2022 Aug 7:119848. Epub 2022 Aug 7.

College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, 100193, China.

Phytogenic allelochemical luteolin has potential to mitigate Microcystis-dominated cyanobacterial blooms (MCBs), but its algicidal effect against toxigenic Microcystis may be impacted by natural factors, especially nitrogen (N) level in waters. This study innovatively explored N-dependent effect of luteolin on Microcystis growth and its microcystins (MCs) production/release, and elucidated underlying mechanisms from proteomics and gene expression views. Generally, at each N level, rising luteolin dose progressively inhibited Microcystis growth by inhibiting proteins syntheses and genes expression involving light-capturing, photosynthetic electron transfer, Calvin cycle and phosphorus (P) acquisition, according to comparative proteomics and gene expression. Read More

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Combined MEK and STAT3 inhibition uncovers stromal plasticity by enriching for cancer-associated fibroblasts with mesenchymal stem cell-like features to overcome immunotherapy resistance in pancreatic cancer.

Gastroenterology 2022 Aug 7. Epub 2022 Aug 7.

Department of Surgery, University of Miami Miller School of Medicine, Miami, FL, USA; Sylvester Comprehensive Cancer Center, Miami, FL, USA. Electronic address:

Background: We have shown that reciprocally activated RAS/MEK and JAK/STAT3 pathways mediate therapeutic resistance in pancreatic ductal adenocarcinoma (PDAC), while combined MEK and STAT3 inhibition (MEKi+STAT3i) overcomes such resistance and alters stromal architecture. We now determine if MEKi+STAT3i reprograms the cancer-associated fibroblast (CAF) and immune microenvironment to overcome resistance to immune checkpoint inhibition in PDAC.

Methods: CAF and immune cell transcriptomes in MEKi (trametinib)+STAT3i(ruxolitinib)- vs. Read More

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Fine particulate matter induces heart defects via AHR/ROS-mediated endoplasmic reticulum stress.

Chemosphere 2022 Aug 7:135962. Epub 2022 Aug 7.

Suzhou Medical College of Soochow University, Suzhou, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, China. Electronic address:

Accumulating body of evidence indicates that exposure to fine particulate matter (PM) is closely associated with congenital heart disease in the offspring, but the underlying molecular mechanisms remain to be elucidated. We previously reported that extractable organic matter (EOM) from PM induces reactive oxygen species (ROS) overproduction by activating aromatic hydrocarbon receptor (AHR), leading to heart defects in zebrafish embryos. We hypothesized that endoplasmic reticulum (ER) stress might be elicited by the excessive ROS production and thereby contribute to the cardiac developmental toxicity of PM. Read More

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Novel Antagonist of the Type 2 Lysophosphatidic Acid Receptor (LPA), UCM-14216, Ameliorates Spinal Cord Injury in Mice.

J Med Chem 2022 Aug 10. Epub 2022 Aug 10.

Departamento de Química Orgánica I, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Madrid E-28040, Spain.

Spinal cord injuries (SCIs) irreversibly disrupt spinal connectivity, leading to permanent neurological disabilities. Current medical treatments for reducing the secondary damage that follows the initial injury are limited to surgical decompression and anti-inflammatory drugs, so there is a pressing need for new therapeutic strategies. Inhibition of the type 2 lysophosphatidic acid receptor (LPA) has recently emerged as a new potential pharmacological approach to decrease SCI-associated damage. Read More

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Bone Loss in Intestinal Inflammation Disease Yields to Osteoclastogenesis Inhibition.

Authors:
Warren Strober

Cell Mol Gastroenterol Hepatol 2022 Aug 7. Epub 2022 Aug 7.

Mucosal Immunity Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. Electronic address:

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Comprehensive Transcriptomic Analysis of Novel Class I HDAC Proteolysis Targeting Chimeras (PROTACs).

Biochemistry 2022 Aug 10. Epub 2022 Aug 10.

Department of Molecular and Cell Biology, University of Leicester, Leicester LE1 7RH, U.K.

The class I histone deacetylase (HDAC) enzymes;HDAC1,2 and 3 form the catalytic engine of at least seven structurally distinct multiprotein complexes in cells. These molecular machines play a vital role in the regulation of chromatin accessibility and gene activity via the removal of acetyl moieties from lysine residues within histone tails. Their inhibition via small molecule inhibitors has beneficial effects in a number of disease types, including the clinical treatment of hematological cancers. Read More

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Associations between isoflavone exposure and reproductive damage in adult males: evidence from human and model system studies.

Biol Reprod 2022 Aug 10. Epub 2022 Aug 10.

Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Gaotanyan St 30, Chongqing 400038, China.

It's controversial whether exposure to isoflavones, constituents of certain plants such as soy bean, exerts male reproductive toxicity. This study was designed to investigate whether isoflavone exposure during adulthood could have deleterious impacts on male reproductive health by the cross-sectional study, animal experiments, and in vitro tests. In the cross-sectional study, we observed that urinary isoflavones were not significantly associated with semen quality including sperm concentrations, sperm count, progressive motility, and total motility, respectively (All P-value for trend>0. Read More

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The transcription factor E2F1 controls the GLP-1 receptor pathway in pancreatic β cells.

Cell Rep 2022 Aug;40(6):111170

Université de Lille, INSERM, CNRS, CHU Lille, Institut Pasteur de Lille, U1283 - UMR 8199 - EGID, 59000 Lille, France; Université de Lille, INSERM, CHU Lille, Institut Pasteur de Lille, U1167 - RID-AGE - Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, 59000 Lille, France. Electronic address:

The glucagon-like peptide 1 (Glp-1) has emerged as a hormone with broad pharmacological potential in type 2 diabetes (T2D) treatment, notably by improving β cell functions. The cell-cycle regulator and transcription factor E2f1 is involved in glucose homeostasis by modulating β cell mass and function. Here, we report that β cell-specific genetic ablation of E2f1 (E2f1) impairs glucose homeostasis associated with decreased expression of the Glp-1 receptor (Glp1r) in E2f1 pancreatic islets. Read More

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Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity.

Cell Rep 2022 Aug;40(6):111167

Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, UK. Electronic address:

Many bacterial pathogens antagonize host defense responses by translocating effector proteins into cells. It remains an open question how those pathogens not encoding effectors counteract anti-bacterial immunity. Here, we show that Klebsiella pneumoniae exploits the evolutionary conserved innate protein SARM1 to regulate negatively MyD88- and TRIF-governed inflammation, and the activation of the MAP kinases ERK and JNK. Read More

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Arabidopsis thaliana serine hydroxymethyltransferases: functions, structures, and perspectives.

Plant Physiol Biochem 2022 Jul 31;187:37-49. Epub 2022 Jul 31.

Synchrotron Radiation Research Section of MCL, National Cancer Institute, Argonne, IL, USA; Department of Structural Biology of Eukaryotes, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland. Electronic address:

Serine hydroxymethyltransferase (SHM) is one of the hallmarks of one-carbon metabolism. In plants, isoforms of SHM participate in photorespiration and/or transfer the one-carbon unit from L-serine to tetrahydrofolate (THF), hence producing 5,10-CH-THF that is needed, e.g. Read More

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Gaining insight into the role of FoxO1 in the progression of disuse-induced skeletal muscle atrophy.

Adv Biol Regul 2022 Jul 31;85:100903. Epub 2022 Jul 31.

Department of Paediatrics, University of Oxford, Children's Hospital, John Radcliffe, Oxford, OX3 9DU, UK; Institute of Developmental and Regenerative Medicine, Roosevelt Dr, IMS-Tetsuya Nakamura Building, Oxford, OX3 7TY, UK; MDUK Oxford Neuromuscular Centre, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK. Electronic address:

Expression of FoxO transcription factors increases during certain forms of atrophy. In a dephosphorylated state, FoxOs participate in ubiquitin-mediated proteasomal degradation through the transcriptional activation of E3-ubiquitin ligases such as MAFbx/atrogin-1 and MuRF1. There is exhaustive research demonstrating that FoxO3a is sufficient to induce MAFbx/atrogin-1 and MuRF-1 expressions. Read More

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The association between personality traits and placebo effects: a preregistered systematic review and meta-analysis.

Pain 2022 Aug 10. Epub 2022 Aug 10.

Department of Psychology, University of Oslo, Norway.

Abstract: Placebo effects are ubiquitous yet highly variable between individuals, and therefore strongly impact clinical trial outcomes such as pain relief. It is unclear whether dispositional psychological traits influence responsiveness to placebo. This preregistered meta-analysis and systematic review synthesized the literature investigating the association between personality traits and placebo effects. Read More

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The Effect of Acute Administration of the Aqueous Calyx Extract of Hibiscus sabdariffa on Blood pressure, Heart rate and Double Product of Apparently Healthy Human Subjects during Different Postures.

Niger J Physiol Sci 2022 Jun 30;37(1):147-152. Epub 2022 Jun 30.

University of Ibadan, Nigeria.

The aqueous calyx extract of Hibiscus sabdariffa (HS) is widely consumed as a beverage in Northern Nigeria and other parts of the world. HS has been reported to lower blood pressure (BP) in animals and man. However, not much is known about the effect of HS on BP in different postures. Read More

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A novel gedunin-2-hydroxypropyl-β-cyclodextrin inclusion complex improves anti-nociceptive and anti-inflammatory activities of gedunin in rodents.

Niger J Physiol Sci 2022 Jun 30;37(1):9-19. Epub 2022 Jun 30.

University of Ilorin, Ilorin.

Gedunin is a bioactive compound, obtained from Entandrophragma angolense (EA), which has limited therapeutic usefulness due to poor aqueous solubility and first-pass effects. Cyclodextrins are cyclic oligosaccharides that form complexes with poorly soluble compounds, thus enhancing their pharmacological activity. In this article, we evaluated the pharmacological activities of gedunin-2-hydroxypropyl-β-cyclodextrin complex (GCD) in rodents. Read More

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Nonlinear manipulation and analysis of large DNA datasets.

Nucleic Acids Res 2022 Aug 10. Epub 2022 Aug 10.

B CUBE, Center for Molecular Bioengineering, Technische Universität Dresden, Dresden, Germany.

Information processing functions are essential for organisms to perceive and react to their complex environment, and for humans to analyze and rationalize them. While our brain is extraordinary at processing complex information, winner-take-all, as a type of biased competition is one of the simplest models of lateral inhibition and competition among biological neurons. It has been implemented as DNA-based neural networks, for example, to mimic pattern recognition. Read More

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The hypotensive effect of the aqueous calyx extract of Hibiscus sabdariffa may occur through the attenuation of Autonomic nervous system activity.

Niger J Physiol Sci 2021 Dec 31;36(2):203-209. Epub 2021 Dec 31.

Usmanu Danfodiyo University, Sokoto, Nigeria.

This study tested the hypothesis that the hypotensive effect of the aqueous calyx extract of Hibiscus sabdariffa (HS) occurs through autonomic mechanisms that may be associated with a reduction in the double product (DP) of the heart. Experiments were performed in accordance with the Principles of the Declaration of Helsinki. Following ethical approval and informed consent, the Harvard step test (HST) was performed in healthy subjects (n=14) to activate the autonomic nervous system before and after the oral administration of 15mg/kg HS. Read More

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December 2021

Differentiation of crescent-forming kidney progenitor cells into podocytes attenuates severe glomerulonephritis in mice.

Sci Transl Med 2022 Aug 10;14(657):eabg3277. Epub 2022 Aug 10.

Excellence Center for Research, Transfer and High Education for the Development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, Italy.

Crescentic glomerulonephritis is characterized by vascular necrosis and parietal epithelial cell hyperplasia in the space surrounding the glomerulus, resulting in the formation of crescents. Little is known about the molecular mechanisms driving this process. Inducing crescentic glomerulonephritis in two Pax2Cre reporter mouse models revealed that crescents derive from clonal expansion of single immature parietal epithelial cells. Read More

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Neuroinflammation inhibition by small-molecule targeting USP7 noncatalytic domain for neurodegenerative disease therapy.

Sci Adv 2022 Aug 10;8(32):eabo0789. Epub 2022 Aug 10.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.

Neuroinflammation is a fundamental contributor to progressive neuronal damage, which arouses a heightened interest in neurodegenerative disease therapy. Ubiquitin-specific protease 7 (USP7) has a crucial role in regulating protein stability in multiple biological processes; however, the potential role of USP7 in neurodegenerative progression is poorly understood. Here, we discover the natural small molecule eupalinolide B (EB), which targets USP7 to inhibit microglia activation. Read More

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Plasticity in astrocyte subpopulations regulates heroin relapse.

Sci Adv 2022 Aug 10;8(32):eabo7044. Epub 2022 Aug 10.

Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA.

Opioid use disorder (OUD) produces detrimental personal and societal consequences. Astrocytes are a major cell group in the brain that receives little attention in mediating OUD. We determined how astrocytes and the astroglial glutamate transporter, GLT-1, in the nucleus accumbens core adapt and contribute to heroin seeking in rats. Read More

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Newly synthesized mRNA escapes translational repression during the acute phase of the mammalian unfolded protein response.

PLoS One 2022 10;17(8):e0271695. Epub 2022 Aug 10.

Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, Ohio, United States of America.

Endoplasmic Reticulum (ER) stress, caused by the accumulation of misfolded proteins in the ER, elicits a homeostatic mechanism known as the Unfolded Protein Response (UPR). The UPR reprograms gene expression to promote adaptation to chronic ER stress. The UPR comprises an acute phase involving inhibition of bulk protein synthesis and a chronic phase of transcriptional induction coupled with the partial recovery of protein synthesis. Read More

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MiR-100-5p inhibits osteogenic differentiation of human bone mesenchymal stromal cells by targeting TMEM135.

Hum Cell 2022 Aug 10. Epub 2022 Aug 10.

Department of Endocrinology, The Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China.

Osteoporosis is a disorder characterized by reduced bone mass, disruption of bone microarchitecture, and a propensity to fracture. The osteogenic differentiation of human bone mesenchymal stromal cells (hBMSCs) exerts a critical effect on preventing bone loss during osteoporosis. Herein, the study recognized miR-100-5p as a deregulated miRNA during osteoporosis (upregulated) and BMSC osteogenic differentiation (downregulated). Read More

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Mercury (Hg) Detection in Aqueous Media, Photocatalyst, and Antibacterial Applications of CdTe/ZnS Quantum Dots.

J Fluoresc 2022 Aug 10. Epub 2022 Aug 10.

Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.

In the present work, CdTe/ZnS high luminescence quantum dots (QDs) were synthesized by a facile, fast, one-pot, and room temperature photochemical method. Synthesized QDs were characterized by different structural and optical analyses such as X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FT-IR), Raman, photoluminescence (PL) and UV-visible (UV-vis) spectroscopies. The results confirmed the successful growth of the ZnS shell and formation of CdTe/ZnS core/shell structure. Read More

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