Cancer Sci 2021 Jul 12. Epub 2021 Jul 12.
Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.
Although the role of bromodomain-containing protein 4 (BRD4) in ovarian cancer, pancreatic cancer, multiple lymphoma and many other diseases is well known, its function in cutaneous melanoma is only partially understood. Here, our results show that the BRD4 inhibitor JQ1 promotes the apoptosis of B16 melanoma cells by altering mitochondrial dynamics, thereby inducing mitochondrial dysfunction and increasing oxidative stress. We found that treatment of B16 cells with different concentrations of JQ1 (125 nM or 250 nM), significantly downregulated the expression of protein subunits involved in mitochondrial respiratory chain complexes I, III, IV, and V, increased reactive oxygen species (ROS), induced energy metabolism dysfunction, significantly enhanced apoptosis and activated the mitochondrial apoptosis pathway. Read More