23,952 results match your criteria inhibit bacterial

4-Chloro-2-Isopropyl-5-Methylphenol Exhibits Antimicrobial and Adjuvant Activity against Methicillin-Resistant .

J Microbiol Biotechnol 2022 May 6;32(6):1-10. Epub 2022 May 6.

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.

Methicillin-resistant (MRSA) causes severe infections and poses a global healthcare challenge. The utilization of novel molecules which confer synergistical effects to existing MRSA-directed antibiotics is one of the well-accepted strategies in lieu of de novo development of new antibiotics. Thymol is a key component of the essential oil of plants in the Thymus and Origanum genera. Read More

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Thymol Reduces -Mediated Virulence Factor Phenol-Soluble Modulin Production in .

Biomed Res Int 2022 9;2022:8221622. Epub 2022 May 9.

Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul 05355, Republic of Korea.

is a major human bacterial pathogen that carries a large number of virulence factors. Many virulence factors of are regulated by the accessory gene regulator () quorum-sensing system. Phenol-soluble modulins (PSMs) are one of the -mediated virulence determinants known to play a significant role in pathogenesis. Read More

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Computational screening of natural compounds as putative quorum sensing inhibitors targeting drug resistance bacteria: Molecular docking and molecular dynamics simulations.

Comput Biol Med 2022 Jun 12;145:105517. Epub 2022 Apr 12.

Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Center of Artificial Intelligence in Precision Medicines, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.

Quorum sensing (QS) is a bacterial communication strategy controlling cells density, biofilm formation, virulence, sporulation, and survival. Since QS is considered a virulence factor in drug-resistant pathogenic bacteria, inhibition of QS can contribute to control the spread of these bacteria. We propose in this study to test in silico, 19 natural compounds for their potential to inhibit QS transcriptional regulators of Pseudomonas aeruginosa (LasR and PqsE) and Chromobacterium violaceum (CviR and CviR'). Read More

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Bioactive Glass in a Multi Drug Resistance Osteomyelitis in Diabetic Foot: Case Report.

Int J Low Extrem Wounds 2022 May 18:15347346221102643. Epub 2022 May 18.

60259Department of Systems Medicine, 9318University of Rome "Tor Vergata", Rome, Italy.

Diabetic foot osteomyelitis (DFO) is a clinical problem with high risk of amputation. The treatment of DFO is still an unsolved challenge. Surgical therapy, antibiotic therapy or conservative treatment are still debated for the timing and the consequences. Read More

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Afr J Infect Dis 2022 6;16(2):71-79. Epub 2022 May 6.

Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia.

Background: Development a granuloma model resembling latent tuberculosis is needed with a fast and efficient time to be used as an effective therapy. This study aimed to form efficient granulomas, increase cellular immunity and humoral immunity, and evaluate growth on media using recombinant protein antibody Ag38kDa, Rifampicin, and a combination of both. Peripheral Blood Mononuclear Cell (PBMC) is derived from a healthy individual separated from monocytes and lymphocytes. Read More

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Degree of Gelatination on Ag-Nanoparticles to Inactivate Multi-drug Resistant Bacterial Biofilm Isolated from Sewage Treatment Plant.

Curr Drug Deliv 2022 May 9. Epub 2022 May 9.

Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai 603 103.

Introduction: Overuse and improper dosage of antibiotics have generated antimicrobial resistance (AMR) worldwide. Pseudomonas aeruginosa (PA), a well-known bacterial strain can establish MDR leading to a variety of infections in humans. Furthermore, these PA strains hold the ability to form biofilms by generating extracellular polymeric substances on the surface of medical tools and critical care units. Read More

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Optimization of the antimicrobial peptide Bac7 by deep mutational scanning.

BMC Biol 2022 May 16;20(1):114. Epub 2022 May 16.

Bioprocess Laboratory, Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.

Background: Intracellularly active antimicrobial peptides are promising candidates for the development of antibiotics for human applications. However, drug development using peptides is challenging as, owing to their large size, an enormous sequence space is spanned. We built a high-throughput platform that incorporates rapid investigation of the sequence-activity relationship of peptides and enables rational optimization of their antimicrobial activity. Read More

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Coumarin thiazoles as unique structural skeleton of potential antimicrobial agents.

Bioorg Chem 2022 May 10;124:105855. Epub 2022 May 10.

Institute of Bioorganic & Medicinal Chemistry, Key Laboratory of Applied Chemistry of Chongqing Municipality, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address:

A novel type of coumarin thiazoles as unique multi-targeting antimicrobial agents were developed through four steps including cyclization, nucleophilic substitution and condensation starting from commercial resorcine. Most of the prepared coumarin thiazoles displayed favorable inhibitory potency against the tested strains. Noticeably, methyl oxime V-a exerted potent inhibitory efficacy against methicillin-resistant Staphylococcus aureus (MRSA) at low concentration (1 μg/mL) and showed broad antimicrobial spectrum. Read More

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Lipid-rich endo-metabolites from a vertically transmitted fungal endophyte Penicillium sp. PM031 attenuate virulence factors of phytopathogenic Ralstonia solanacearum.

Microbiol Res 2022 May 10;261:127058. Epub 2022 May 10.

Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address:

The bacterial wilt caused by Ralstonia solanacearum is a destructive plant disease globally. Since a completely non-biological control measure could be a matter of environmental concern, investigations of developing eco-friendly strategies are required to control this phytopathogen. Attenuation of the bacterial virulence in addition to destroying the pathogen may be an alternative and overarching approach to control this disease. Read More

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A novel antibacterial gold nanoparticle layer with a self-cleaning ability through the production of oxygen bubbles.

J Mater Chem B 2022 May 16. Epub 2022 May 16.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, P. R. China.

Bacterial contamination of medical devices not only constitutes a serious threat to the health of patients, but also promotes the evolution of bacterial drug-resistance. Here, a new strategy to fabricate a highly efficient self-cleaning antibacterial coating is reported. Briefly, a nano-topological structure is formed on the substrate surface by the deposition of a gold nanoparticle layer (GNPL), and then antimicrobial peptide mimetics (AMPMs) with optimized structure and catalase (CAT) are modified on the surface. Read More

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in Cancer Therapy: Current Knowledge, Challenges and Future Perspectives.

Front Oncol 2022 28;12:891187. Epub 2022 Apr 28.

School of Art & Design, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.

Drug resistance, undesirable toxicity and lack of selectivity are the major challenges of conventional cancer therapies, which cause poor clinical outcomes and high mortality in many cancer patients. Development of alternative cancer therapeutics are highly required for the patients who are resistant to the conventional cancer therapies, including radiotherapy and chemotherapy. The success of a new cancer therapy depends on its high specificity to cancer cells and low toxicity to normal cells. Read More

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Traditional Chinese Medicine Tanreqing Targets Both Cell Division and Virulence in .

Front Cell Infect Microbiol 2022 27;12:884045. Epub 2022 Apr 27.

Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China.

has been recognized as an important human pathogen and poses a serious health threat worldwide. With the advent of antibiotic resistance, such as the increased number of methicillin-resistant (MRSA), there is an urgent need to develop new therapeutical agents. In this study, Chinese traditional medicine Tanreqing (TRQ) has been used as an alternative treating agent against MRSA and we aim to unravel the mode of action of TRQ underlying MRSA inhibition. Read More

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A Surface Exposed, Two-Domain Lipoprotein Cargo of a Type XI Secretion System Promotes Colonization of Host Intestinal Epithelia Expressing Glycans.

Front Microbiol 2022 29;13:800366. Epub 2022 Apr 29.

Department of Microbiology, The University of Tennessee, Knoxville, Knoxville, TN, United States.

The only known required component of the newly described Type XI secretion system (TXISS) is an outer membrane protein (OMP) of the DUF560 family. TXISS are broadly distributed across proteobacteria, but properties of the cargo proteins they secrete are largely unexplored. We report biophysical, histochemical, and phenotypic evidence that NilC is surface exposed. Read More

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Using to Model Therapeutic Interventions of Neurodegenerative Diseases Targeting Microbe-Host Interactions.

Front Pharmacol 2022 28;13:875349. Epub 2022 Apr 28.

School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China.

Emerging evidence from both clinical studies and animal models indicates the importance of the interaction between the gut microbiome and the brain in the pathogenesis of neurodegenerative diseases (NDs). Although how microbes modulate neurodegeneration is still mostly unclear, recent studies have started to probe into the mechanisms for the communication between microbes and hosts in NDs. In this review, we highlight the advantages of using (C. Read More

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Anti-virulence properties of catechin-in-cyclodextrin-in-phospholipid liposome through down-regulation of gene expression in MRSA strains.

Microb Pathog 2022 May 13;167:105585. Epub 2022 May 13.

Chemical Biology Lab (ASK-II-409), School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamilnadu, India. Electronic address:

Methicillin resistant Staphylococcus aureus (MRSA) is a prime pathogen responsible for various infections in human beings. Expression of virulence factors is a biggest challenge in MRSA, which results in failure of conventional antibiotic therapy. In connection to the search for natural and safe anti-virulence compounds, the present study focused to evaluate the anti-virulence potential of catechin-in-cyclodextrin-in-phospholipid liposome (CCPL) on MRSA strains. Read More

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An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation.

J Nanobiotechnology 2022 May 14;20(1):232. Epub 2022 May 14.

Department of Orthopaedics, First Affiliated Hospital of University of Science and Technology of China, Hefei, 230001, China.

Background: The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function and potential infection risk. Immune interventions in diabetic wounds face a possible contradiction between simultaneous establishment of the pro-inflammatory microenvironment in response to potential bacterial invasion and the anti-inflammatory microenvironment required for tissue repair. To study this contradiction and accelerate diabetic-wound healing, we developed a photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres ([email protected]) and silver nanoparticles (Ag NPs). Read More

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Improvement of the Cd and Zn phytoremediation efficiency of rice (Oryza sativa) through the inoculation of a metal-resistant PGPR strain.

Chemosphere 2022 May 11;302:134900. Epub 2022 May 11.

School of Environmental Science and Engineering, Tianjin University, 92 Weijin Road, Tianjin, 300072, China. Electronic address:

Cadmium (Cd) and zinc (Zn) in contaminated soil inhibit rice yield and produce toxic effects on human body through rice accumulation. Plant growth promoting rhizobacteria (PGPR) assisted phytoremediation is an effective ecological measure to improve the remediation efficiency of heavy metal contaminated soil. The purpose of this study was to investigate the efficiency of the combination of rice and Cd/Zn-tolerant PGPR strain Bacillus sp. Read More

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Identification, expression patterns, evolutionary characteristics and recombinant protein activities analysis of CD209 gene from Megalobrama amblycephala.

Fish Shellfish Immunol 2022 May 11. Epub 2022 May 11.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China. Electronic address:

CD209 is a type II transmembrane protein in the C-type lectin family, which is involved in the regulation of innate and adaptive immune system. Although it has been widely studied in mammals, but little has been reported about fish CD209 genes. In the present study, Megalobrama amblycephala CD209 (MaCD209) gene was cloned and characterized, its expression patterns, evolutionary characteristics, agglutinative and bacteriostatic activities were analyzed. Read More

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A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.

Cell 2022 May 11. Epub 2022 May 11.

Department of Infectious Diseases, School of Immunology and Microbial Sciences, King's College London, London, UK. Electronic address:

Interferons (IFNs) induce an antimicrobial state, protecting tissues from infection. Many viruses inhibit IFN signaling, but whether bacterial pathogens evade IFN responses remains unclear. Here, we demonstrate that the Shigella OspC family of type-III-secreted effectors blocks IFN signaling independently of cell death inhibitory activity. Read More

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Pogostone Enhances the Antibacterial Activity of Colistin against MCR-1-Positive Bacteria by Inhibiting the Biological Function of MCR-1.

Molecules 2022 Apr 28;27(9). Epub 2022 Apr 28.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

The emergence of the plasmid-mediated colistin resistance gene has resulted in the loss of available treatments for certain severe infections. Here we identified a potential inhibitor of MCR-1 for the treatment of infections caused by MCR-1-positive drug-resistant bacteria, especially MCR-1-positive carbapenem-resistant (CRE). A checkerboard minimum inhibitory concentration (MIC) test, a killing curve test, a growth curve test, bacterial live/dead assays, scanning electron microscope (SEM) analysis, cytotoxicity tests, molecular dynamics simulation analysis, and animal studies were used to confirm the in vivo/in vitro synergistic effects of pogostone and colistin. Read More

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Essential Oil: Exploring the Antibacterial Multitarget Mechanisms, Chemcomputational Toxicity Prediction, and Safety Assessment in Zebrafish Embryos.

Molecules 2022 Apr 19;27(9). Epub 2022 Apr 19.

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Center of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Nowadays, increasing interest has recently been given to the exploration of new food preservatives to avoid foodborne outbreaks or food spoilage. Likewise, new compounds that substitute the commonly used synthetic food preservatives are required to restrain the rising problem of microbial resistance. Accordingly, the present study was conducted to examine the chemical composition and the mechanism(s) of action of the essential oil (CSEO) against Typhimuriumand . Read More

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Anti-Inflammatory and Anti-Bacterial Potential of Mulberry Leaf Extract on Oral Microorganisms.

Int J Environ Res Public Health 2022 Apr 20;19(9). Epub 2022 Apr 20.

Department of Dental Hygiene, Konyang University, Daejeon 35365, Korea.

Mulberry leaves extract ( extracts; MAE) is known to have therapeutic potentials for numerous human diseases, including diabetes, neurological disorders, cardiovascular diseases, and cancers. However, there has not been sufficient research proving therapeutic effects on oral disease and its related oral risk factors. Thus, we investigated whether MAE has any anti-inflammatory and anti-bacterial effects on risk factors causing oral infectious diseases. Read More

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Characterization of Interactions between CTX-M-15 and Clavulanic Acid, Desfuroylceftiofur, Ceftiofur, Ampicillin, and Nitrocefin.

Int J Mol Sci 2022 May 7;23(9). Epub 2022 May 7.

Department of Chemistry, Washington State University, Pullman, WA 99164, USA.

Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamases (ESBLs) are commonly associated with Gram-negative, hospital-acquired infections worldwide. Several beta-lactamase inhibitors, such as clavulanate, are used to inhibit the activity of these enzymes. To understand the mechanism of CTX-M-15 activity, we have determined the crystal structures of CTX-M-15 in complex with two specific classes of beta-lactam compounds, desfuroylceftiofur (DFC) and ampicillin, and an inhibitor, clavulanic acid. Read More

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Evaluation of a Gel Containing a Extract in an In Vivo Model of Wound Healing.

Int J Mol Sci 2022 Apr 24;23(9). Epub 2022 Apr 24.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31-98166 Messina, Italy.

Inappropriate wound healing (WH) management can cause significant comorbidities, especially in patients affected by chronic and metabolic diseases, such as diabetes. WH involves several different, partially overlapping processes, including hemostasis, inflammation, cell proliferation, and remodeling. Oxidative stress in WH contributes to WH impairment because of the overexpression of radical oxygen species (ROS) and nitrogen species (RNS). Read More

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Isolation of Potato Endophytes and Screening of Antimicrobial Genes.

Int J Mol Sci 2022 Apr 21;23(9). Epub 2022 Apr 21.

Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.

Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophytes from potato tubers. Read More

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-Induced Receptor-like Kinase Genes Deletion Provides Broad-Spectrum Resistance to Bacterial Blight in Rice.

Int J Mol Sci 2022 Apr 20;23(9). Epub 2022 Apr 20.

College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.

Rice bacterial blight caused by pv. () seriously affects rice yield production. The discovery and application of broad-spectrum resistance genes are of great advance for disease resistance breeding. Read More

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Human α-Defensin-6 Neutralizes Toxins TcdA and TcdB by Direct Binding.

Int J Mol Sci 2022 Apr 19;23(9). Epub 2022 Apr 19.

Institute of Pharmacology and Toxicology, Ulm University Medical Center, 89081 Ulm, Germany.

Rising incidences and mortalities have drawn attention to infections (CDIs) in recent years. The main virulence factors of this bacterium are the exotoxins TcdA and TcdB, which glucosylate Rho-GTPases and thereby inhibit Rho/actin-mediated processes in cells. This results in cell rounding, gut barrier disruption and characteristic clinical symptoms. Read More

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Biofilm-Associated Agr and Sar Quorum Sensing Systems of Are Inhibited by 3-Hydroxybenzoic Acid Derived from .

ACS Omega 2022 May 20;7(17):14653-14665. Epub 2022 Apr 20.

Infection Biology, Department of Life Sciences, Central University of Tamil Nadu, Neelakudi, Tiruvarur 610005, Tamilnadu, India.

Biofilm-producing () is less sensitive to conventional antibiotics than free-living planktonic cells. Here, we evaluated the antibiofilm activity of () and one of its constituent compounds 3-hydroxybenzoic acid (3-HBA) against multi-drug-resistant . We performed gas chromatography-mass spectroscopy (GC-MS) to identify the major constituents in the methanolic extract of . Read More

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An improved selective/differential medium for culturing the group from wastewater.

Anal Methods 2022 May 13. Epub 2022 May 13.

Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-720 Olsztyn, Poland.

The aim of this study was to develop an effective selective/differential medium for culturing environmental strains of the group (BFG). This goal was achieved by modifying standard commercial Bile Esculin Agar (BBE Agar). Bile Esculin Agar was combined with substances that inhibit the growth of non-BFG bacteria, mostly and . Read More

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A designed self-microemulsion delivery system for dihydromyricetin and its dietary intervention effect on high-fat-diet fed mice.

Food Chem 2022 Apr 11;390:132954. Epub 2022 Apr 11.

College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China. Electronic address:

The present study aims to design a self-microemulsion delivery system (d-α-tocopheryl polyethylene glycol 1000 succinate - quillaja saponin) to enhance the absorptivity of dihydromyricetin (DMY-S), and to investigate its dietary intervention effect on high-fat-diet (HFD) fed mice. We find DMY-S can inhibit the increase of body weight and fat mass, preventing non-alcoholic fatty liver disease. Compared to the model group, the abundance of mice intestinal flora is mainly changed in certain bacterial genera of Firmicutes and Bacteroides, including norank_f_Muribaculaceae and Blautia. Read More

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