Food Chem 2021 Mar 13;357:129588. Epub 2021 Mar 13.
National Food Institute, Technical University of Denmark, Research Group for Bioactives - Analysis and Application, Kemitorvet, 2800 Kgs. Lyngby, Denmark. Electronic address:
Deuterated vitamin D standards are used commonly as internal standards in LC-MS/MS analysis of vitamin D and 25-hydroxyvitamin D in food. However, the use of various eluent additives, such as methylamine, formic acid and ammonium formate, also contributes to matrix effects and the performance of analysis by affecting accuracy and robustness. For the first time, continuous post-column infusion experiments of isotopically labelled vitamin D-[d] were performed to evaluate ion-suppression in a wide variety of food (salmon, cheese, pork fat, pork meat, and egg yolk). Read More