1,434 results match your criteria infiltration epidermis

A simple and efficient agroinfiltration method for transient gene expression in Citrus.

Plant Cell Rep 2021 May 4. Epub 2021 May 4.

Citrus Research and Education Center, University of Florida/IFAS, Lake Alfred, FL, USA.

Key Message: Microwounding pre-treatment facilitates agroinfiltration and transient gene expression in hard-to-agroinfiltrate citrus varieties. Agrobacterium infiltration is a widely used method for transient expression studies in plants, but this method is not used extensively in citrus because of its low efficiency. In this study, we developed an easy, cheap, and reliable agroinfiltration method for transient gene expression in citrus. Read More

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Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice.

Molecules 2021 Apr 27;26(9). Epub 2021 Apr 27.

Department of Dermatology, Medical University of Warsaw, 02-091 Warsaw, Poland.

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. Read More

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Preparation of Topical Itraconazole with Enhanced Skin/Nail Permeability and In Vivo Antifungal Efficacy against Superficial Mycosis.

Pharmaceutics 2021 Apr 27;13(5). Epub 2021 Apr 27.

Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Jeonnam 58554, Korea.

In this study, a stable and highly skin-permeable topical delivery system for itraconazole (ITZ) was designed to provide effective treatment against superficial mycosis. Herein, ITZ was incorporated into a solution composed of ethanol, benzyl alcohol, hydrochloric acid, Transcutol P, and cyclomethicone as a delivery vehicle, solubilizer, protonating agent, permeation enhancer, and spreading agent, respectively. At 72 h, the optimal topical ITZ formulation (ITZ-TF#11) exhibited 135% enhanced skin permeability, which led to increases in drug deposition in the stratum corneum, epidermis, and dermis of 479%, 739%, and 2024%, respectively, compared with the deposition of 1% ITZ in ethanol (control). Read More

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A Case of Generalized Lichen Sclerosus et Atrophicus.

Ann Dermatol 2020 Aug 30;32(4):327-330. Epub 2020 Jun 30.

Department of Dermatology, College of Medicine, Hallym University, Anyang, Korea.

A 62-year-old female, with previous history of asthma and hypertension, presented with generalized hyperpigmented skin lesion, found a year ago. Physical examination revealed brown colored lichenified and sclerotic patches on the lower abdomen and flexural areas of extremities. Punch biopsy was performed and histopathological examination revealed hyperkeratosis, follicular plugging and thinning in epidermis. Read More

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Improved imiquimod-induced psoriasis like dermatitis using microneedles in mice.

Eur J Pharm Biopharm 2021 Apr 22. Epub 2021 Apr 22.

Beijing Laboratory of Biomedical Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P.R. China. Electronic address:

Psoriasis is a chronic inflammatory skin disease, in which the key features are epidermis hyperplasia, hyper-keratinization, leading to low drug absorption. As an approach of transdermal drug delivery, the microneedle (MN) has received increasing attentions for its painless penetration and efficient administration. In this study, we fabricated polylactic acid polymer MNs with hot-press method and established a psoriasis-like skin inflammation model in ear and dorsal skin of mice by topical application of imiquimod (IMQ). Read More

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Licoricidin Abrogates T Cell Activation by Modulating PTPN1 Activity and Attenuates Atopic Dermatitis In Vivo.

J Invest Dermatol 2021 Apr 12. Epub 2021 Apr 12.

School of Life Sciences, Immune Synapse and Cell Therapy Research Center, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.

Licoricidin, the fifth-highest fraction among the isolated 48 molecules from Glycyrrhiza uralensis extracts, has been known as anti-inflammatory bioactive molecules, however, few studies have shown its inhibitory effect on T cell activation and atopic dermatitis. The present study examined the therapeutic potential of licoricidin in atopic dermatitis by modulating T cell activation with molecular mechanism. Licoricidin attenuated the expression of IL-2 mRNA in stimulated T cells without cytotoxicity. Read More

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High-Frequency Ultrasound for Evaluation of the Pathological Invasion Level of Extramammary Paget Disease.

J Ultrasound Med 2021 Apr 15. Epub 2021 Apr 15.

Department of Medical Ultrasound, Shanghai Skin Disease Hospital, Ultrasound Research and Education Institute, Tongji University School of Medicine, Shanghai, China.

Objectives: Pathological invasion level of extramammary Paget disease (EMPD) is strongly related with its risk staging, treatment, and prognosis. However, the current evaluation before treatments fails to evaluate pathological invasion level of EMPD. High-frequency ultrasound (HFUS) may play a key role to solve this problem. Read More

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The Histogenetic Model of Melanoma in the Modern Era of Personalized Medicine.

Acta Dermatovenerol Croat 2020 Dec;28(7):236-237

Prof. Luca Roncati, MD, DMLS, PhD , Polyclinic Hospital, Largo del Pozzo 71 - 41124 , Modena (MO), Italy;

Malignant melanoma (M) can be defined, quite simply, as a malignant neoplasm derived from melanocytes; however, there is great histological and, consequently, clinical variability from case to case (1). In order to try to overcome this intrinsic difficulty, various classification systems have been proposed over the years; as part of this effort, the World Health Organization (WHO) introduced its famous classification about half a century ago (2). Currently, the International Classification of Diseases for Oncology (ICD-O), provided by the WHO International Agency for Research on Cancer (IARC), distinguishes the in situ forms from invasive ones, recognizing four main morphological subtypes: nodular M, superficial spreading M, lentigo maligna M, and acral lentiginous M (3). Read More

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December 2020

Effect of on DNCB-Induced Allergic Contact Dermatitis-Like Skin Lesion in Balb/c Mice.

Nutrients 2021 Mar 4;13(3). Epub 2021 Mar 4.

Department of Anatomy, College of Korean Medicine, Kyung Hee University, 26, Kyunghee dae-ro, Dongdaemun-gu, Seoul 02447, Korea.

(CP), or Korean red pinecone, is a cluster of fruit. CP has also been verified in several studies to have anti-oxidation, anti-fungal, anti-bacterial, and anti-melanogenic effects. However, anti-inflammatory effects have not yet been confirmed in the inflammatory responses of pinecones to allergic contact dermatitis. Read More

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Increased Therapeutic Efficacy of SLN Containing Etofenamate and Ibuprofen in Topical Treatment of Inflammation.

Pharmaceutics 2021 Mar 3;13(3). Epub 2021 Mar 3.

Faculty of Pharmacy, Research Institute for Medicines (iMed.ULisboa), Universidade de Lisboa, 1649-003 Lisbon, Portugal.

Innovative formulations, including solid lipid nanoparticles (SLNs), have been sought to improve skin permeation of non-steroidal anti-inflammatory drugs (NSAIDs). The present study explores the use of SLNs, prepared using a fusion-emulsification method, to increase skin permeation and in vivo activity of two relevant NSAIDs: A liquid molecule (etofenamate) and a solid one (ibuprofen), formulated in a 2% hydroxypropyl methylcellulose gel through the gelation of SLN suspensions. Compritol 888 ATO and Tween 80 were used as a solid lipid and a surfactant, respectively. Read More

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Not All Cases of Mammary Paget's Disease are Cytokeratin-7 Positive: A Challenging Diagnosis!

Int J Surg Pathol 2021 Mar 22:10668969211002920. Epub 2021 Mar 22.

Department of Pathology, Cliniques Universitaires Saint-Luc, Brussels, Belgium.

Mammary Paget's disease accounts for 1% to 3% of all breast tumors and manifests as a chronic eczematous lesion of the areolar skin. It can occur without any underlying neoplasia or can be present in association with an underlying invasive and/or in situ carcinoma of the breast. The present report describes a challenging nipple punch biopsy showing an infiltration of the lower third to two-thirds of the epidermis by large, ovoid, neoplastic cells. Read More

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T cell infiltration on local CpG-B delivery in early-stage melanoma is predominantly related to CLEC9ACD141 cDC1 and CD14 antigen-presenting cell recruitment.

J Immunother Cancer 2021 Mar;9(3)

Department of Medical Oncology, Amsterdam University Medical Centers, Vrije Universiteit, Cancer Center Amsterdam, Amsterdam, The Netherlands

Background: We previously reported CpG-B injection at the primary tumor excision site prior to re-excision and sentinel node biopsy to result in immune activation of the sentinel lymph node (SLN), increased melanoma-specific CD8 T cell rates in peripheral blood, and prolonged recurrence-free survival. Here, we assessed recruitment and activation of antigen-presenting cell (APC) subsets in the SLN and at the injection site in relation to T cell infiltration.

Methods: Re-excision skin specimens from patients with clinical stage I-II melanoma, collected 7 days after intradermal injection of either saline (n=10) or 8 mg CpG-B (CPG7909, n=12), were examined by immunohistochemistry, quantifying immune subsets in the epidermis, papillary, and reticular dermis. Read More

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Lumenato protects normal human dermal fibroblasts from neutrophil-induced collagen-3 damage in co-cultures.

PLoS One 2021 17;16(3):e0248183. Epub 2021 Mar 17.

Immunology and Infectious Diseases Laboratory, Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Collagen is the major structural protein in the extracellular matrix of skin produced by fibroblasts. UV exposure results in infiltration of neutrophils within the epidermis and dermis, inducing collagen damage and contributing to the process of photo-aging. Collagen-3 is an integral structural component with collagen-1, and is an important regulator of collagen-1 fibrillogenesis. Read More

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Derma-Hc, a New Developed Herbal Formula, Ameliorates Cutaneous Lichenification in Atopic Dermatitis.

Int J Mol Sci 2021 Feb 26;22(5). Epub 2021 Feb 26.

Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.

Atopic dermatitis (AD) is a chronic cutaneous disorder that is characterized by severe eczematous inflammation, swelling, and lichenification. Activation of T helper (Th)-22 cells by allergens leads to epidermal hyperplasia with hyperkeratosis at the chronic phase of AD. Derma-Hc is composed of five natural herbs with anti-AD effects, such as BUNGE, Briq. Read More

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February 2021

Tropical ulcers: the first imported cases and review of the literature.

Eur J Dermatol 2021 Feb;31(1):75-80

U.O. Dermatology, ASST Valle Olona, Presidio di Gallarate, Italy.

Background: A tropical ulcer is a bacterial necrotizing disease of the skin, with an acute or chronic clinical course, caused by anaerobic bacteria, notably Fusobacteria spp.

Objectives: We present six Italian tourists who acquired tropical ulcers in tropical and subtropical countries.

Materials & Methods: Four males and two females acquired a skin ulcer during trips to Brazil, Malaysia, Fiji Islands, Zambia, Tanzania and India. Read More

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February 2021

The frequently dismissed importance of properly sampling skin bruises.

Leg Med (Tokyo) 2021 May 23;50:101867. Epub 2021 Feb 23.

LABANOF, Laboratorio di Antropologia e Odontologia Forense, Sezione di Medicina Legale, Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, via Luigi Mangiagalli 37, 20133 Milan, Italy.

The correct interpretation of skin lesions is a crucial issue in forensic medicine. Many macroscopic and microscopic vital reaction markers and molecules have been studied in the past years in order to estimate the timing of injuries. However, literature lacks information regarding how to perform sampling to obtain a more reliable and accurate response. Read More

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Factors Affecting the Efficacy of Suction Curettage Using an Arthroscopic Shaver for Bromhidrosis.

Dermatol Surg 2021 02;47(2):245-249

Concordia Middle School, Chia-Yi County, Taiwan.

Background: Suction-curettage using an arthroscopic shaver is the most effective surgical treatment for bromhidrosis; however, information regarding the procedure is limited. This study investigated the factors that affect the efficacy of suction-curettage.

Patients And Methods: We retrospectively evaluated data for 215 patients (430 axillae) with bromhidrosis treated with suction-curettage using an arthroscopic shaver between 2011 and 2019. Read More

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February 2021

Epithelialized tunnels are a source of inflammation in hidradenitis suppurativa.

J Allergy Clin Immunol 2021 Feb 3. Epub 2021 Feb 3.

Laboratory of Investigative Dermatology, The Rockefeller University, New York, NY. Electronic address:

Background: Hidradenitis suppurativa (HS), also known as acne inversa, is a chronic, painful, and burdensome inflammatory disease manifesting in nodules and abscesses, with progression to chronically draining tunnels in later-stage disease.

Objective: We sought to determine whether HS tunnels are immunologically active participants in disease activity.

Methods: Skin biopsy specimens were obtained by using ultrasound guidance in untreated patients with HS and those enrolled in an open-label study of brodalumab (ClinicalTrials. Read More

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February 2021

Sophoricoside from Styphnolobium japonicum improves experimental atopic dermatitis in mice.

Phytomedicine 2021 Feb 14;82:153463. Epub 2021 Jan 14.

Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. Electronic address:

Background: Abnormal immune responses, specifically excessive differentiation of Th2 cells, are associated with the development of atopic dermatitis (AD). Sophoricoside, the genistein-4'-β-D-glucoside isolated from Styphnolobium japonicum, has previously demonstrated anti-inflammatory and immunosuppressive effects along with IL-3 and IL-5 inhibitory activities. Therefore, we speculated that sophoricoside could regulate AD by regulating abnormal immune responses. Read More

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February 2021

Pentacyclic triterpene compounds from loquat leaves reduce skin inflammation and epidermal hyperplasia in psoriasis via inhibiting the Th17 cells.

Mol Immunol 2021 04 2;132:30-40. Epub 2021 Feb 2.

Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China; Key Laboratory of Tropical Disease Control (Sun Yat-Sen University), Ministry of Education, Guangzhou, China. Electronic address:

Psoriasis is a refractory inflammatory skin disease affecting 2 %-3 % of the world population, characterized by the infiltration and hyper-proliferation of inflammatory cells and aberrant differentiation of keratinocytes. Targeting the IL-23/ Th17 axis has been well recognized as a promising therapeutic strategy, as the IL-23/ Th17 signal plays a vital role in the pathology of psoriasis. Three pentacyclic triterpene compounds isolated from loquat leaves have been reported with significant inhibitory effects on RORγt transcription activity and Th17 cell differentiation, and excellent performance in preventing lupus nephritis pathogenesis. Read More

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Role of CYR61 in psoriatic lesional and perilesional skin: A clinical and immunohistochemical study.

J Cosmet Dermatol 2021 Jan 22. Epub 2021 Jan 22.

Department of Pathology, Faculty of Medicine, Menoufia University, Shebin El Kom, Egypt.

Background: Psoriasis is considered as an immune-mediated disorder with significant epidermal hyperplasia and inflammation. Cysteine-rich angiogenic inducer 61 (CYR61), known as CCN family member 1 (CCN1), plays an important role in cell proliferation and neovascularization which may trigger psoriasis development.

Aims: This study aimed to assess the immunostaining of CYR61 in psoriatic skin (lesional and perilesional) compared to control skin. Read More

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January 2021

DZ2002 alleviates psoriasis-like skin lesions via differentially regulating methylation of GATA3 and LCN2 promoters.

Int Immunopharmacol 2021 Feb 4;91:107334. Epub 2021 Jan 4.

Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Zhangjiang, Shanghai 201203, China; University of Chinese Academy of Sciences, NO.19A Yuquan Road, Beijing 100049, China; Laboratory of Immunology and Virology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Psoriasis is the most prevalent inflammatory skin disorders, affecting 1-3% of the worldwide population. We previously reported that topical application of methyl 4-(adenin-9-yl)-2-hydroxybutanoate (DZ2002), a reversible S-adenosyl-l-homocysteine hydrolase (SAHH) inhibitor, was a viable treatment in murine psoriatic skin inflammation. In current study, we further explored the mechanisms of DZ2002 on keratinocyte dysfunction and skin infiltration, the key pathogenic events in psoriasis. Read More

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February 2021

Effects of wet-to-dry bandages on second intention healing of surgical wounds on the skin of goats.

J Adv Vet Anim Res 2020 Dec 7;7(4):647-654. Epub 2020 Oct 7.

Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Objectives: This study aimed to examine the effects of wet-to-dry bandages for the treatment of surgical wounds in goats.

Materials And Methods: Three types of bandages, i.e. Read More

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December 2020

Immune responses and therapeutic options in psoriasis.

Cell Mol Life Sci 2021 Mar 2;78(6):2709-2727. Epub 2021 Jan 2.

Unit of Molecular Signal Transduction in Inflammation, Center for Inflammation Research, Ghent University - VIB, Technologiepark 71, B-9052, Ghent, Belgium.

Psoriasis is a chronic inflammatory disease of the skin that affects about 2-3% of the population and greatly impairs the quality of life of affected individuals. Psoriatic skin is characterized by excessive proliferation and aberrant differentiation of keratinocytes, as well as redness caused by increased dilation of the dermal blood vessels and infiltration of immune cells. Although the pathogenesis of psoriasis has not yet been completely elucidated, it is generally believed to arise from a complex interplay between hyperproliferating keratinocytes and infiltrating, activated immune cells. Read More

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Protocol for Flow Cytometric Detection of Immune Cell Infiltration in the Epidermis and Dermis of a Psoriasis Mouse Model.

STAR Protoc 2020 Dec 17;1(3):100115. Epub 2020 Sep 17.

Shanghai Institute of Immunology, Translational Medicine Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Psoriasis is an incurable chronic inflammatory skin disorder. The imiquimod (IMQ)-induced mouse model of psoriasis is the most widely used model for drug discovery and pre-clinical studies of psoriasis. The inflamed and thickened skin frequently compromises the quality of single-cell suspensions generated from IMQ-induced skin lesions, which has an impact on subsequent analyses by flow cytometry. Read More

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December 2020

Reactive perforating leprosy: A rare case report of type 2 leprosy reaction.

Int J Mycobacteriol 2020 Oct-Dec;9(4):451-453

Department of Dermatology and Venereology, Faculty of Medicine, Padjadjaran University, Dr Hasan Sadikin Hospital, Bandung, Indonesia.

Reactive perforating leprosy (RPL) is a rare clinical manifestation of type 2 leprosy reaction. A case of lepromatous leprosy (LL) with type 2 leprosy reaction presented as RPL in one patient was reported. A physical examination showed multiple punched-out ulcers with regular border, without undermined edge, and dermal base. Read More

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December 2020

Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study.

Mater Sci Eng C Mater Biol Appl 2021 Feb 1;119:111586. Epub 2020 Oct 1.

Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UK. Electronic address:

The combination of oral antidiabetic drugs, pioglitazone, metformin, and glibenclamide, which also exhibit the strongest anti-inflammatory action among oral antidiabetic drugs, were loaded into chitosan/gelatin/polycaprolactone (PCL) by electrospinning and polyvinyl pyrrolidone (PVP)/PCL composite nanofibrous scaffolds by pressurized gyration to compare the diabetic wound healing effect. The combination therapies significantly accelerated diabetic wound healing in type-1 diabetic rats and organized densely packed collagen fibers in the dermis, it also showed better regeneration of the dermis and epidermis than single drug-loaded scaffolds with less inflammatory cell infiltration and edema. The formation of the hair follicles started in 14 days only in the combination therapy and lower proinflammatory cytokine levels were observed compared to single drug-loaded treatment groups. Read More

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February 2021

Small interfering RNA targeting of keratin 17 reduces inflammation in imiquimod-induced psoriasis-like dermatitis.

Chin Med J (Engl) 2020 Nov 20;133(24):2910-2918. Epub 2020 Nov 20.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Background: Psoriasis is a common chronic inflammatory skin disease with 2% to 3% prevalence worldwide and a heavy social-psychological burden for patients and their families. As the exact pathogenesis of psoriasis is still unknown, the current treatment is far from satisfactory. Thus, there is an urgent need to find a more effective therapy for this disease. Read More

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November 2020

B-Cell Acute Lymphoblastic Leukemia Presenting as Leukemia Cutis: A Case Report.

Cureus 2020 Oct 19;12(10):e11032. Epub 2020 Oct 19.

Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, USA.

Leukemia cutis (LC) is a manifestation of leukemia with infiltration of the dermis, epidermis, or subcutis by malignant leukocytes resulting in papules, plaques, nodules, or ulcers. It is usually associated with acute and chronic myeloid leukemia as well as T-cell acute lymphoblastic leukemia (T-ALL) but is very rare in patients with B-cell acute lymphoblastic leukemia (B-ALL). We report a case of a 58-year-old Hispanic male who presented with a non-healing leg ulcer of three months along with patches on the face, left arm, and bilateral legs with white blood cell (WBC) count of 50800/mm with 83% blasts, and flow cytometry findings of B-ALL. Read More

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October 2020

Mesenchymal Stem Cells Application in Wound Tissue Healing in Old Animals.

Stem Cells Cloning 2020 11;13:103-116. Epub 2020 Nov 11.

Department of Hospital Surgery №1, Pirogov Russian National Research Medical University, Moscow, Russia.

Purpose: An assessment of the effectiveness of progenitor mesenchymal stem cell as injections and as part of a polymer hydrogel for the wounds treatment.

Materials And Methods: Fixed-size wounds (average area of 135.8 mm) were modeled on the back of white Wistar rats, aged 9 months. Read More

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November 2020