19,935 results match your criteria inexpensive assay

C3dg Quantification by PEG Precipitation and or TRIFMA.

Methods Mol Biol 2021 ;2227:43-49

Department of Biomedicine, Aarhus University, Aarhus, Denmark.

Detection of complement activation products can be carried out in a number of ways, and different methods are used in different laboratories. No international standard for measuring complement activation in the clinical setting has been agreed upon.Here we describe a modified assay for measuring C3dg. Read More

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January 2021

Adsorptive decontamination of paper mill effluent by nano fly ash: response surface methodology, adsorption isotherm and reusability studies.

Water Sci Technol 2021 Apr;83(7):1662-1676

Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125 Noida 201313, Uttar Pradesh, India E-mail:

In the present study, adsorption of colour and other pollutants from agro-based paper mill effluent onto fabricated coal fly ash nanoparticles (CFA-N) have been investigated. Response surface methodology was applied to evaluate the operational conditions for maximum ouster of colour from effluent by nano structured CFA-N. Maximum reduction in colour (92. Read More

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Generalized Rhythmic Delta Activity Frontally Predominant Differentiates Dementia With Lewy Bodies From Alzheimer's Disease and Parkinson's Disease Dementia: A Conventional Electroencephalography Visual Analysis.

Clin EEG Neurosci 2021 Apr 12:1550059421997147. Epub 2021 Apr 12.

Neurology Unit, 18630Azienda Ospedaliero-Universitaria of Parma, Parma, Emilia-Romagna, Italy.

An easily accessible and inexpensive neurophysiological technique such as conventional electroencephalography may provide an accurate and generally applicable biomarker capable of differentiating dementia with Lewy bodies (DLB) from Alzheimer's disease (AD) and Parkinson's disease-associated dementia (PDD). We carried out a retrospective visual analysis of resting-state electroencephalography (EEG) recording of 22 patients with a clinical diagnosis of 19 probable and 3 possible DLB, 22 patients with probable AD and 21 with PDD, matched for age, duration, and severity of cognitive impairment. By using the grand total EEG scoring method, the total score and generalized rhythmic delta activity frontally predominant (GRDAfp) alone or, even better, coupled with a slowing of frequency of background activity (FBA) and its reduced reactivity differentiated DLB from AD at an individual level with an high accuracy similar to that obtained with quantitative EEG (qEEG). Read More

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Green Synthesized Silver Nanoparticles Using Leaf Extract, Evaluation of Their Antibacterial, Anticancer and Antioxidant Activities.

Iran J Pharm Res 2020 ;19(4):306-320

Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, School of Pharmacy , Mazandaran University of Medical Sciences, Sari, Iran .

Biogenic synthesis of silver nanoparticles (SNPs) has great attention of scientists, as it provides clean, biocompatible, non-toxic and inexpensive fabrication. In this study, leaf extract was used for synthesizing SNPs which reduces silver nitrate into silver zero-valent. SNPs were characterized by UV, FTIR, XRD, SEM-EDS, and TEM analysis. Read More

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January 2020

Monitoring Sleep Changes via a Smartphone App in Bipolar Disorder: Practical Issues and Validation of a Potential Diagnostic Tool.

Front Psychiatry 2021 24;12:641241. Epub 2021 Mar 24.

Department of Psychiatry & Psychotherapeutic Medicine, Medical University Graz, Graz, Austria.

Sleep disturbances are common early warning signs of an episode of bipolar disorder, and early recognition can favorably impact the illness course. Symptom monitoring via a smartphone app is an inexpensive and feasible method to detect an early indication of changes such as sleep. The study aims were (1) to assess the acceptance of apps and (2) to validate sleeping times measured by the smartphone app . Read More

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Rapid Point-of-Care Testing for SARS-CoV-2 Virus Nucleic Acid Detection by an Isothermal and Nonenzymatic Signal Amplification System Coupled with a Lateral Flow Immunoassay Strip.

Sens Actuators B Chem 2021 Apr 3:129899. Epub 2021 Apr 3.

Medical School of Southeast University, Nanjing, 210009, People's Republic of China.

An outbreak of a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began in December 2019. Accurate, rapid, convenient, and relatively inexpensive diagnostic methods for SARS-CoV-2 infection are important for public health and optimal clinical care. The current gold standard for diagnosing SARS-CoV-2 infection is reverse transcription-polymerase chain reaction (RT-PCR). Read More

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[Contribution of respiratory relaxation techniques during intravitreal injections: A pilot study].

J Fr Ophtalmol 2021 Apr 8. Epub 2021 Apr 8.

Service de chirurgie ambulatoire, centre hospitalier d'Avranches-Granville, Granville, France. Electronic address:

Purpose: To study the effects of breathing techniques for anxiety, perceived pain, and patient satisfaction while receiving intravitreal injections.

Method: This prospective, randomized clinical study included patients admitted for intravitreal anti-VEGF injections. They were randomized into two groups: a relaxation group who listened to a prerecorded relaxation breathing session before and during the injection, and a control group who received the injection without a relaxation session. Read More

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Prognostic value of D-dimer levels in patients with gastric cancer undergoing gastrectomy.

Surg Oncol 2021 Apr 5;37:101570. Epub 2021 Apr 5.

Department of Surgery, Seoul St. Mary Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea. Electronic address:

Background: Plasma D-dimer levels have been associated with tumor progression and oncological outcomes in several cancers. This study assessed the relationships of D-dimer levels with clinicopathological features and survival outcomes in patients with gastric cancer undergoing gastrectomy.

Methods: Data from 666 patients with gastric cancer who underwent gastrectomy between June 2012 and December 2015 were collected and analyzed; these data were acquired during a previous randomized clinical trial (PROTECTOR trial, NCT01448746). Read More

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Development and clinical validation of loop-mediated isothermal amplification (LAMP) assay to diagnose high HBV DNA levels in resource-limited settings.

Clin Microbiol Infect 2021 Apr 7. Epub 2021 Apr 7.

Unité d'Epidémiologie des Maladies Émergentes, Institut Pasteur, Paris, France. Electronic address:

Objectives: A massive scale-up of testing and treatment is indicated to globally eliminate hepatitis B virus (HBV) infection. However, access to a polymerase chain reaction (PCR), a key test to quantify HBV DNA levels and determine treatment eligibility, is limited in resource-limited countries. We developed and evaluated loop-mediated isothermal amplification (LAMP) assay to diagnose clinically important HBV DNA thresholds defined by WHO (≥20,000 and ≥200,000 IU/mL). Read More

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Cost savings of paper analytical devices (PADs) to detect substandard and falsified antibiotics: Kenya case study.

Med Access Point Care 2021 Jan-Dec;5. Epub 2021 Mar 8.

Division of Practice Advancement and Clinical Education, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, USA.

Background: Over 10% of antibiotics in low- and middle-income countries (LMICs) are substandard or falsified. Detection of poor-quality antibiotics via the gold standard method, high-performance liquid chromatography (HPLC), is slow and costly. Paper analytical devices (PADs) and antibiotic paper analytical devices (aPADs) have been developed as an inexpensive way to estimate antibiotic quality in LMICs. Read More

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Force transmission analysis of surface coating materials for multi-fingered robotic grippers.

Gökhan Erdemir

PeerJ Comput Sci 2021 18;7:e401. Epub 2021 Mar 18.

Electrical and Electronics Engineering Department, Istanbul Sabahattin Zaim University, İstanbul, Turkey.

Robotic systems are generally used for grasping, carrying, holding, and many similar operations, typically in industrial applications. One of the most important components of robotic systems is robot grippers for the aforementioned operations, which are not only mission-critical but also represent a significant operational cost due to the time and expense associated with replacement. Grasping operations require sensitive and dexterous manipulation ability. Read More

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Hyperspectral interference tomography of nacre.

Proc Natl Acad Sci U S A 2021 Apr;118(15)

Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, WI 53706;

Structural characterization of biologically formed materials is essential for understanding biological phenomena and their enviro-nment, and for generating new bio-inspired engineering concepts. For example, nacre-the inner lining of some mollusk shells-encodes local environmental conditions throughout its formation and has exceptional strength due to its nanoscale brick-and-mortar structure. This layered structure, comprising alternating transparent aragonite (CaCO) tablets and thinner organic polymer layers, also results in stunning interference colors. Read More

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Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of .

Anal Chem 2021 Apr 7. Epub 2021 Apr 7.

Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey.

Several different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage () as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of () (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, concentration, and reaction time. Read More

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MLPA analysis of 32 foetuses with a congenital heart defect and 1 foetus with renal defects - pilot study. The significant frequency rate of presented pathological CNV.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2021 Mar 31. Epub 2021 Mar 31.

Department of Medical Genetics, University Hospital Olomouc, Czech Republic.

Aims: The aim of this retrospective study was to determine the detection rate of the pathogenic copy number variants (CNVs) in a cohort of 33 foetuses - 32 with CHD (congenital heart defects) and 1 with kidney defect, after exclusion of common aneuploidies (trisomy 13, 18, 21, and monosomy X) by karyotyping, Multiplex ligation - dependent probe amplification (MLPA) and chromosomal microarray analysis (CMA). We also assess the effectivity of MLPA as a method of the first tier for quick and inexpensive detection of mutations, causing congenital malformations in foetuses.

Methods: MLPA with probe mixes P070, P036 - Telomere 3 and 5, P245 - microdeletions, P250 - DiGeorge syndrome, and P311 - CHD (Congenital heart defects) was performed in 33 samples of amniotic fluid and chorionic villi. Read More

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Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum.

ACS Sens 2021 Apr 6. Epub 2021 Apr 6.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Rapid and inexpensive serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies are essential to conduct large-scale seroprevalence surveys and can potentially complement nucleic acid or antigen tests at the point of care. During the COVID-19 pandemic, extreme demand for traditional lateral flow tests has stressed manufacturing capacity and supply chains. Motivated by this limitation, we developed a SARS-CoV-2 antibody test using cellulose, an alternative membrane material, and a double-antigen sandwich format. Read More

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Growth of the acetogenic bacterium Acetobacterium woodii on glycerol and dihydroxyacetone.

Environ Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University Frankfurt/Main, ax-von-Laue-Str. 9, 60438, Frankfurt, Germany.

More than two million tons of glycerol are produced during industrial processes each year and, therefore, glycerol is an inexpensive feedstock to produce biocommodities by bacterial fermentation. Acetogenic bacteria are interesting production platforms and there have been few reports in the literature on glycerol utilization by this ecophysiologically important group of strictly anaerobic bacteria. Here, we show that the model acetogen Acetobacterium woodii DSM1030 is able to grow on glycerol, but contrary to expectations, only for 2-3 transfers. Read More

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Robotic Telemedicine for Mental Health: A Multimodal Approach to Improve Human-Robot Engagement.

Front Robot AI 2021 18;8:618866. Epub 2021 Mar 18.

Department of Mechanical Engineering, Imperial College London, and UK Dementia Research Institute-Care Research and Technology Centre, London, United Kingdom.

COVID-19 has severely impacted mental health in vulnerable demographics, in particular older adults, who face unprecedented isolation. Consequences, while globally severe, are acutely pronounced in low- and middle-income countries (LMICs) confronting pronounced gaps in resources and clinician accessibility. Social robots are well-recognized for their potential to support mental health, yet user compliance (i. Read More

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Functional paper-based materials for diagnostics.

ChemTexts 2021 31;7(2):14. Epub 2021 Mar 31.

Merck Lab @ TU Darmstadt, Technische Universität Darmstadt, 64287 Darmstadt, Germany.

Functional papers are the subject of extensive research efforts and have already become an irreplaceable part of our modern society. Among other issues, they enable fast and inexpensive detection of a plethora of analytes and simplify laboratory work, for example in medical tests. This article focuses on the molecular and structural fundamentals of paper and the possibilities of functionalization, commercially available assays and their production, as well as on current and future challenges in research in this field. Read More

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Nanopore sequencing and its application to the study of microbial communities.

Comput Struct Biotechnol J 2021 7;19:1497-1511. Epub 2021 Mar 7.

Research Unit, Hospital Universitario N.S. de Candelaria, Universidad de La Laguna, 38010 Santa Cruz de Tenerife, Spain.

Since its introduction, nanopore sequencing has enhanced our ability to study complex microbial samples through the possibility to sequence long reads in real time using inexpensive and portable technologies. The use of long reads has allowed to address several previously unsolved issues in the field, such as the resolution of complex genomic structures, and facilitated the access to metagenome assembled genomes (MAGs). Furthermore, the low cost and portability of platforms together with the development of rapid protocols and analysis pipelines have featured nanopore technology as an attractive and ever-growing tool for real-time in-field sequencing for environmental microbial analysis. Read More

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A application for automated H-Reflex measurements and analyses.

Biomed Signal Process Control 2021 Apr 22;66. Epub 2021 Feb 22.

Temple University, College of Engineering, Philadelphia, PA, USA.

Objective: H-Reflex is a test that is carried out to measure the relative excitability of reflex pathways. Although reliable, conventional methods consist of performing many small steps, which requires a high level of attentiveness, and thus can carry an elevated risk of human error, despite proper training. Equipment that is available to perform those tests with different levels of automation are typically proprietary, inextensible by the user, and expensive. Read More

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Evaluation of minimal fracture liaison service resource: costs and survival in secondary fracture prevention-a prospective one-year study in South-Finland.

Aging Clin Exp Res 2021 Apr 3. Epub 2021 Apr 3.

National Institute for Health and Welfare, Helsinki, Finland.

Background: Fracture liaison service (FLS) is a secondary prevention model for identification of patients at risk for fragility fractures.

Aims: This study was conducted to evaluate the number and costs of secondary prevention of low-energy fractures in the city of Kouvola in Finland.

Methods: Women aged ≥ 45 years and men ≥ 60 years treated in the emergency department with a low-energy fracture were identified. Read More

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Solvothermal Synthesis of Multiple Dihydropyrimidinones at a Time as Inhibitors of Eg5.

Molecules 2021 Mar 30;26(7). Epub 2021 Mar 30.

Department of Pharmacy, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, China.

Solvothermal synthesis of multiple dihydropyrimidinones at a time has been developed in inexpensive and green bio-based solvent lactic acid without any additional catalysts or additives. By this method, thirty new dihydropyrimidinone derivatives were synthesized in two batches and characterized. All of the compounds were screened by Eg5 motor protein ATPase assay, and the positive compounds were tested against the Caco-2 cell line, HeLa cell line, L929 cell line and T24 cell line in vitro. Read More

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Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2.

Viruses 2021 03 5;13(3). Epub 2021 Mar 5.

CONICET-Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires 1425, Argentina.

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55-100) positive samples and 104/105 (99. Read More

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