3,043 results match your criteria induction foxp3+


Induction of CD4 regulatory T cells by stimulation with Staphylococcal Enterotoxin C2 through different signaling pathways.

Biomed Pharmacother 2021 Sep 21;143:112204. Epub 2021 Sep 21.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China; Key Laboratory of Superantigen Research, Shenyang Bureau of Science and Technology, Shenyang, China.

As a member of superantigens, Staphylococcal Enterotoxin C2 (SEC2) can potently activate T cells expressing specific Vβ repertoires and has been applied in clinic for tumor immunotherapy in China for more than 20 years. However, excessive activation of T cells by over-stimulation with superantigen are always followed by eliciting regulatory T cells (Tregs) induction and functional immunosuppression, which brings uncertainties to SEC2 application in tumor immunotherapy. In this study, we found that SEC2 could induce CD4CD25Foxp3 Tregs from the murine splenocytes in dose and time related manners. Read More

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September 2021

Augmented Expansion of Treg Cells From Healthy and Autoimmune Subjects Adult Progenitor Cell Co-Culture.

Front Immunol 2021 1;12:716606. Epub 2021 Sep 1.

Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, United Kingdom.

Recent clinical experience has demonstrated that adoptive regulatory T (Treg) cell therapy is a safe and feasible strategy to suppress immunopathology induction of host tolerance to allo- and autoantigens. However, clinical trials continue to be compromised due to an inability to manufacture a sufficient Treg cell dose. Multipotent adult progenitor cells (MAPC) promote Treg cell differentiation , suggesting they may be repurposed to enhance expansion of Tregs for adoptive cellular therapy. Read More

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September 2021

Prophylactic and Therapeutic Effects of MOG-Conjugated PLGA Nanoparticles in C57Bl/6 Mouse Model of Multiple Sclerosis.

Adv Pharm Bull 2021 May 26;11(3):505-513. Epub 2020 Jul 26.

Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.

Multiple sclerosis (MS) is a debilitating neuroinflammatory disorder of the central nervous system. It is believed to result from an impaired immune response against myelin components especially myelin oligodendrocyte glycoprotein (MOG). Some efforts have been made to bioconjugate the MOG peptides to tolerogenic particles like poly (lactic-co-glycolic acid) (PLGA) for treating animal models of autoimmune disorders. Read More

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Pre-incubation of corneal donor tissue with sCD83 improves graft survival via the induction of alternatively activated macrophages and tolerogenic dendritic cells.

Am J Transplant 2021 Sep 1. Epub 2021 Sep 1.

Department of Experimental Ophthalmology, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany.

Immune responses reflect a complex interplay of cellular and extracellular components which define the microenvironment of a tissue. Therefore, factors that locally influence the microenvironment and re-establish tolerance might be beneficial to mitigate immune-mediated reactions, including the rejection of a transplant. In this study, we demonstrate that pre-incubation of donor tissue with the immune modulator soluble CD83 (sCD83) significantly improves graft survival using a high-risk corneal transplantation model. Read More

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September 2021

Predictive value of immunological markers after bacille Calmette-Guérin induction in bladder cancer.

BJU Int 2021 Aug 27. Epub 2021 Aug 27.

Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.

Objectives: To investigate the predictive value of different immunological markers on treatment outcomes after bacille Calmette-Guérin (BCG) induction in high-risk non-muscle-invasive bladder cancer (NMIBC).

Patients And Methods: Patients who underwent transurethral resection of bladder tumour for NMIBC were assessed for study eligibility. Urine and blood samples were taken from patients at baseline (immediately before first dose of induction) and after induction (4 h after last [sixth] dose). Read More

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Infection by Strongyloides venezuelensis attenuates chronic colitis induced by Dextran Sodium Sulfate ingestion in BALB/c mice.

Immunobiology 2021 Sep 17;226(5):152129. Epub 2021 Aug 17.

Departamento de Parasitologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil. Electronic address:

Inflammatory bowel diseases (IBD) are chronic health problems of difficult management and treatment. Epidemiological studies indicate an inverse association between helminth infections and IBD, and experimental data confirm that helminth infections modulate the severity of experimental acute colitis in mice. However, the effects of helminth infections on chronic colitis, which is clinically more relevant, have been poorly explored. Read More

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September 2021

Extracellular vesicles derived from small intestinal lamina propria reduce antigen-specific immune response.

Korean J Intern Med 2021 Aug 25. Epub 2021 Aug 25.

Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.

Background/aims: Extracellular vesicles (EVs) are secreted from various types of cells and have specific functions related to their origin. EVs are observed in the small intestinal lamina propria (lpEVs), but their function remains unclear. This study aimed to investigate the role of lpEVs. Read More

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Intestinal Regulatory T Cells as Specialized Tissue-Restricted Immune Cells in Intestinal Immune Homeostasis and Disease.

Front Immunol 2021 4;12:716499. Epub 2021 Aug 4.

Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN, United States.

FOXP3 regulatory T cells (Treg cells) are a specialized population of CD4 T cells that restrict immune activation and are essential to prevent systemic autoimmunity. In the intestine, the major function of Treg cells is to regulate inflammation as shown by a wide array of mechanistic studies in mice. While Treg cells originating from the thymus can home to the intestine, the majority of Treg cells residing in the intestine are induced from FOXP3 conventional CD4 T cells to elicit tolerogenic responses to microbiota and food antigens. Read More

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Nucleic acid vaccines and CpG oligodeoxynucleotides for allergen immunotherapy.

Authors:
Alain Jacquet

Curr Opin Allergy Clin Immunol 2021 Aug 12. Epub 2021 Aug 12.

Center of Excellence in Vaccine Research and Development, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Purpose Of Review: Molecular forms of allergen-specific immunotherapy (AIT) are continuously emerging to improve the efficacy of the treatment, to shorten the duration of protocols and to prevent any side effects. The present review covers the recent progress in the development of AIT based on nucleic acid encoding allergens or CpG oligodeoxynucleotides (CpG-ODN).

Recent Findings: Therapeutic vaccinations with plasmid deoxyribonucleic acid (DNA) encoding major shrimp Met e 1 or insect For t 2 allergen were effective for the treatment of food or insect bite allergy in respective animal models. Read More

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Hyperlipidemia-induced metabolic changes in regulatory T cells result in altered function.

Eur J Immunol 2021 Aug 7. Epub 2021 Aug 7.

Tufts University School of Medicine, and The Graduate School of Biomedical Sciences Boston, Boston, MA, USA.

Regulatory T cells (Tregs) play a critical role in maintaining self-tolerance and controlling inflammation. However, physiologically relevant conditions that alter Treg function and drive disease pathogenesis are poorly understood and few have been defined. We have previously shown that induction of hyperlipidemia in mice results in changes in Tregs that reduce their function. Read More

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Case Report: Regulatory T Cell-Independent Induction of Remission in a Patient With Collagenous Colitis.

Front Med (Lausanne) 2021 19;8:678268. Epub 2021 Jul 19.

Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, Osaka, Japan.

Collagenous colitis (CC), a prototypical microscopic colitis, is a chronic inflammatory disorder of the colon. The diagnosis of CC depends on the pathological examination. The colonic mucosa of patients with CC is characterized by the presence of a substantially thickened collagen band (>10μm) under the surface epithelium. Read More

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Perspective of Immunopathogenesis and Immunotherapies for Kawasaki Disease.

Front Pediatr 2021 19;9:697632. Epub 2021 Jul 19.

Department of Pediatrics, MacKay Memorial Hospital, Taipei, Taiwan.

Kawasaki Disease (KD) is an acute inflammatory illness that mostly occurs in children below 5 years of age, with intractable fever, mucocutaneous lesions, lymphadenopathy, and lesions of the coronary artery (CAL). KD is sharing clinical symptoms with systemic inflammatory syndrome in children (MIS-C) which is related to COVID-19. Certain genes are identified to be associated with KD, but the findings usually differ between countries and races. Read More

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CD4+CD25+CD127-Foxp3+ and CD8+CD28- Tregs in Renal Transplant Recipients: Phenotypic Patterns, Association With Immunosuppressive Drugs, and Interaction With Effector CD8+ T Cells and CD19+IL-10+ Bregs.

Front Immunol 2021 15;12:716559. Epub 2021 Jul 15.

Transplantation Immunology, Institute of Immunology, University Hospital Heidelberg, Heidelberg, Germany.

Introduction: Gaps still exist regarding knowledge on regulatory cells in transplant recipients. We studied the phenotypic patterns of CD4+, CD8+CD28- Tregs, and CD19+IL-10+ Bregs in the blood of healthy controls (HC), end-stage kidney disease patients (ESKD), early and late stable renal transplant recipients (Tx), and transplant recipients with steroid-treated acute cellular rejection 1 week-3 months after successful treatment. We also investigated the relationship between immunosuppressive drugs and the aforementioned regulatory cells in transplant recipients. Read More

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IL-33 Drives Expansion of Type 2 Innate Lymphoid Cells and Regulatory T Cells and Protects Mice From Severe, Acute Colitis.

Front Immunol 2021 15;12:669787. Epub 2021 Jul 15.

Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.

The hallmarks of inflammatory bowel disease are mucosal damage and ulceration, which are known to be high-risk conditions for the development of colorectal cancer. Recently, interleukin (IL)-33 and its receptor ST2 have emerged as critical modulators in inflammatory disorders. Even though several studies highlight the IL-33/ST2 pathway as a key factor in colitis, a detailed mode of action remains elusive. Read More

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Oral probiotic promotes indoleamine 2,3-dioxygenase- and TGF-β-Producing plasmacytoid dendritic cells to initiate protection against type 1 diabetes.

Immunol Lett 2021 Jul 30;239:12-19. Epub 2021 Jul 30.

Department of Infectious Diseases and Immunology, University of Florida, 2015 SW 16th Ave, Gainesville, FL, United States. Electronic address:

Colonization factor antigen I (CFA/I) fimbria, an adhesin from enterotoxigenic Escherichia coli, confers protection in murine autoimmune models for type 1 diabetes (T1D), multiple sclerosis, and rheumatoid arthritis. Although CFA/I fimbriae's initial mode of action is in a bystander or in an antigen (Ag)-independent fashion, protection is ultimately dependent upon the induction and/or activation of auto-Ag-specific regulatory T cells (Tregs). However, little is known about how protection transitions from bystander suppression to Ag-specific Tregs. Read More

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Respiratory syncytial virus nonstructural protein 1 breaks immune tolerance in mice by downregulating Tregs through TSLP-OX40/OX40L-mTOR axis.

Mol Immunol 2021 Oct 28;138:20-30. Epub 2021 Jul 28.

Department of Pediatrics, Children's Digital Health and Data Center, Zhongnan Hospital of Wuhan University, Wuhan, China. Electronic address:

Respiratory syncytial virus (RSV) infection in early life is associated strongly with the subsequent development and exacerbation of asthma, however, the mechanism is still ambiguous. In this study, we identified that RSV nonstructural protein (NS) 1 plays a critical role. Plasmid-mediated overexpression of NS1 induced significant airway hyperresponsiveness, eosinophilia, and mucus hyperproduction in mice. Read More

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October 2021

Transcriptional and posttranslational regulation of Th17/Treg balance in health and disease.

Eur J Immunol 2021 Sep 15;51(9):2137-2150. Epub 2021 Aug 15.

Department of Immunology and Microbiology, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Regulatory T (Treg) cells and T helper type 17 (Th17) cells play important roles in adaptive immune responses, antagonizing each other in immune disorders. Th17/Treg balance is critical to maintaining the immune homeostasis of human bodies and is tightly regulated under healthy conditions. The transcription factors that are required for driving Th17 and Treg cell lineages differentiation respectively, RORγt and FOXP3 are tightly regulated under different tissue microenvironment, especially the transcriptional induction, posttranslational modifications, and dynamic enzymatic cofactors binding. Read More

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September 2021

NR4A3 Mediates Thymic Negative Selection.

J Immunol 2021 08 26;207(4):1055-1064. Epub 2021 Jul 26.

Maisonneuve-Rosemont Hospital Research Center, Montreal, Quebec, Canada;

Central tolerance aims to limit the production of T lymphocytes bearing TCR with high affinity for self-peptide presented by MHC molecules. The accumulation of thymocytes with such receptors is limited by negative selection or by diversion into alternative differentiation, including T regulatory cell commitment. A role for the orphan nuclear receptor NR4A3 in negative selection has been suggested, but its function in this process has never been investigated. Read More

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In Vivo Induction of Regulatory T Cells Via CTLA-4 Signaling Peptide to Control Autoimmune Encephalomyelitis and Prevent Disease Relapse.

Adv Sci (Weinh) 2021 07 5;8(14):2004973. Epub 2021 May 5.

Department of Life Science College of Natural Sciences Hanyang University Research institute for Natural Sciences Research Institute for Convergence of Basic Sciences Hanyang University Seoul 04763 Republic of Korea.

Regulatory T cells play a key role in immune tolerance to self-antigens, thereby preventing autoimmune diseases. However, no drugs targeting Treg cells have been approved for clinical trials yet. Here, a chimeric peptide is generated by conjugation of the cytoplasmic domain of CTLA-4 (ctCTLA-4) with dNP2 for intracellular delivery, dNP2-ctCTLA-4, and evaluated Foxp3 expression during Th0, Th1, Treg, and Th17 differentiation dependent on TGF-. Read More

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The Role of Regulatory T Cells in Epicutaneous Immunotherapy for Food Allergy.

Front Immunol 2021 8;12:660974. Epub 2021 Jul 8.

Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

In recent decades, a rapid increase in the prevalence of food allergies has led to extensive research on novel treatment strategies and their mechanisms. Mouse models have provided preliminary insights into the mechanism of epicutaneous immunotherapy (EPIT)-induced immune tolerance. In EPIT, antigen applied on the skin surface can be captured, processed, and presented in the lymph nodes (LNs) by Antigen-presenting cells (APCs). Read More

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Effects of Atorvastatin on T-Cell Activation and Apoptosis in Systemic Lupus Erythematosus and Novel Simulated Interactions With C-Reactive Protein and Interleukin 6.

ACR Open Rheumatol 2021 Sep 23;3(9):642-653. Epub 2021 Jul 23.

Karolinska Institutet, Stockholm, Sweden.

Objective: We study activation of T helper 17 (Th17) and regulatory T (Treg) cells and induction of apoptosis in cells from patients with systemic lupus erythematosus (SLE) compared with controls and effects of atorvastatin and its simulated interactions with other compounds.

Methods: Mononuclear cells from 10 patients with SLE and 10 controls were cultured in conditions that induce Th17 and/or Treg cell polarization and/or apoptosis and were studied by FACScan. Gene expression was determined by quantitative real-time reverse transcription-polymerase chain reaction. Read More

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September 2021

Programmed death ligand 1 (PD-L1) plays a vital part in DC tolerogenicity induced by IFN-γ.

Int Immunopharmacol 2021 Oct 20;99:107978. Epub 2021 Jul 20.

Blood Transfusion Center of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia.

Interferon-γ (IFN-γ) is the sole representative of type II IFNs, with well recognized role in numerous inflammatory processes. Lately, its significant pleiotropic nature has been recognized in many scenarios, where IFN-γ contributes to maintenance or induction of tolerogenic responses in context of various immune cell types. In this manuscript we demonstrate, that IFN-γ-mediated induction of programmed death ligand 1 (PD-L1) on human monocyte-derived dendritic cells (DCs) represents an important tolerogenic aspect in immunological network of type II IFNs. Read More

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October 2021

BCG therapy is associated with long-term, durable induction of Treg signature genes by epigenetic modulation.

Sci Rep 2021 Jul 22;11(1):14933. Epub 2021 Jul 22.

Immunobiology Laboratories, Massachusetts General Hospital, Bldg 149, 13th Street, Boston, MA, 02116, USA.

Induction of immunosuppressive T-regulatory cells (Tregs) is a desirable goal in autoimmunity, and perhaps other immune diseases of activation. One promising avenue is with the bacille-calmette-guérin (BCG) vaccine in autoimmune type 1 diabetes (T1D). Its administration is associated with gradual clinical improvements in human autoimmunity over a 2-3 year post-vaccination period. Read More

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Enhanced Susceptibility of Galectin-1 Deficient Mice to Experimental Colitis.

Front Immunol 2021 28;12:687443. Epub 2021 Jun 28.

Division of Hematopoietic Innovative Therapies, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain.

Galectin-1 is a -galactoside-binding lectin, ubiquitously expressed in stromal, epithelial, and different subsets of immune cells. Galectin-1 is the prototype member of the galectin family which shares specificity with -galactoside containing proteins and lipids. Immunomodulatory functions have been ascribed to endogenous galectin-1 due to its induction of T cell apoptosis, inhibitory effects of neutrophils and T cell trafficking. Read More

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Conversion of Anergic T Cells Into Foxp3 IL-10 Regulatory T Cells by a Second Antigen Stimulus .

Front Immunol 2021 25;12:704578. Epub 2021 Jun 25.

Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.

T cell anergy is a common mechanism of T cell tolerance. However, although anergic T cells are retained for longer time periods in their hosts, they remain functionally passive. Here, we describe the induction of anergic CD4 T cells by intravenous application of high doses of antigen and their subsequent conversion into suppressive Foxp3 IL-10 Tr1 cells but not Foxp3 Tregs. Read More

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Systemic T Cell Subsets and Cytokines in Patients With Homozygous Sickle Cell Disease and Asymptomatic Urinary Tract Infections in Togo.

Ochsner J 2021 ;21(2):163-172

Unité de Recherche en Immunologie et Immuno-modulation (UR2IM)/Laboratoire de Microbiologie et de Contrôle Qualité des Denrées Alimentaires (LAMICODA)/Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA)-Université de Lomé, Togo, West Africa.

In sickle cell disease (SCD), cytokine expression influences the pivotal pathways that contribute to disease pathogenesis. Additional infection could affect the immune profile of patients with SCD and increase disease mortality. The aim of this study was to investigate the cytokines and T helper cells profile in patients with asymptomatic urinary tract infection and homozygous SCD (HbSS). Read More

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January 2021

Prophylactic Dendritic Cell Vaccination in Experimental Breast Cancer Controls Immunity and Hepatic Metastases.

Anticancer Res 2021 Jul;41(7):3419-3427

Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Triangulo Mineiro (Universidade Federal do Triângulo Mineiro, UFTM), Uberaba, MG, Brazil;

Background/aim: Liver metastases are among the principal mortality causes in cancer patients. Dendritic cell immunotherapies have shown promising results in some tumors by mediating immunological mechanisms that could be involved in liver metastases during primary tumor growth. The present study aimed to evaluate the impact of prophylactic dendritic cell vaccination on the liver of mice with 4T1 mouse breast carcinoma. Read More

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MiR-146a regulates regulatory T cells to suppress heart transplant rejection in mice.

Cell Death Discov 2021 Jun 17;7(1):165. Epub 2021 Jun 17.

Department of General Surgery, Tianjin Medical University General Hospital, Anshan Road, Tianjin, 300052, China.

Regulatory T cells (Tregs), which characteristically express forkhead box protein 3 (Foxp3), are essential for the induction of immune tolerance. Here, we investigated microRNA-146a (miR-146a), a miRNA that is widely expressed in Tregs and closely related to their homeostasis and function, with the aim of enhancing the function of Tregs by regulating miR-146a and then suppressing transplant rejection. The effect of the absence of miR-146a on Treg function in the presence or absence of rapamycin was detected in both a mouse heart transplantation model and cell co-cultures in vitro. Read More

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Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis.

Cell Mol Gastroenterol Hepatol 2021 Jun 29;12(4):1251-1266. Epub 2021 Jun 29.

BC Children's Hospital Research Institute, University of British Columbia, Vancouver, British Columbia, Canada; Division of Gastroenterology, Hepatology and Nutrition, BC Children's Hospital, Vancouver, Canada. Electronic address:

Background & Aims: Exclusive enteral nutrition (EEN) is used to treat pediatric Crohn's disease (CD), but therapeutic benefits are variable, and EEN can lead to microbial dysbiosis. Because of reported lower efficacy EEN is not routinely used to treat pediatric ulcerative colitis (UC). Inulin-type fructans (IN) beneficially modulate the gut microbiome and promote expansion of anti-inflammatory immune cells. Read More

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Forkhead Box P3 (Foxp3) serum level and Foxp3 Gene-Promoter polymorphisms in Egyptian rheumatoid arthritis patients: A case-control study.

Egypt J Immunol 2021 Apr;28(2):53-64

Department of Medical Microbiology & Immunology , Faculty of Medicine ,Zagazig University , Zagazig, Egypt.

Regulatory T (Treg) cells are the chief player in induction of autotolerance and the transcription factor, Forkhead Box P3 (Foxp3), is the master regulator of their development and function. Polymorphisms in Foxp3 locus affect Foxp3 expression and can influence Treg cell function. This study aimed to determine the frequency of -3279C/A and -924A/G polymorphisms in the promoter region of the Foxp3 gene in Egyptian rheumatoid arthritis (RA) patients in comparison to apparently healthy controls, to test their association with Foxp3 serum levels as well as with patients' clinical and laboratory features. Read More

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