207,729 results match your criteria incubation reduction

Differential effects of androgens and estrogens over cellular GH sensitivity in HEPG2 cells.

Growth Horm IGF Res 2021 May 4;57-58:101390. Epub 2021 May 4.

Institute of Maternal and Child Research (IDIMI), School of Medicine, University of Chile, Santiago, Chile.

Testosterone and estrogen concentrations progressively increase during puberty, and in association with growth hormone (GH), lead to the increase in height velocity known as the pubertal growth spurt. Very limited information is available however, regarding the possible effects of sex steroids over GH cellular sensitivity.

Objective: To investigate the effects of different concentrations of testosterone, estradiol and dihydrotestosterone over the GH intracellular signaling pathway. Read More

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Effect of different rearing system on eggs production, hatchability, and offspring quality in layer breeders.

Poult Sci 2021 Mar 11;100(6):101101. Epub 2021 Mar 11.

Department of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec 36 A, Magdalenka, Poland.

This study analyzed the influence of an aviary system, in comparison with battery cages, on rearing and reproduction of parent-stock (PS) laying-type chickens. ISA Brown PS chicks were reared for 16 wk in battery cages or in an aviary system. Chickens reared in cages were kept there throughout the rearing period, whereas those reared in the aviary were released after 7 wk. Read More

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Modulations of lipid metabolism and development of the Atlantic salmon (Salmo salar) fry in response to egg-to-fry rearing conditions.

Fish Physiol Biochem 2021 May 11. Epub 2021 May 11.

Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, University of Namur (UNamur), 5000, Namur, Belgium.

In stocking program, the use of artificial incubation conditions in hatcheries from the fertilisation of eggs to the release of unfed fry could reduce their ability to adapt to the natural environment. This study evaluates the effects of three factors on the fitness and physiology of salmon fry at their emergence, the origin of water (river vs drilling), the type of support in the incubator (support matrix vs plastic sheets) and the type of incubators (Californian vs vertical trays), and compares them to a semi-natural incubation method in river. Key biological functions including nutritional and immune status were compared among experimental conditions using biometric parameters, lipid composition and gene expression analyses. Read More

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Rapid in vitro differentiation of bacteria by ion mobility spectrometry.

Appl Microbiol Biotechnol 2021 May 11. Epub 2021 May 11.

MicroDiagnostics, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.

Rapid screening of infected people plays a crucial role in interrupting infection chains. However, the current methods for identification of bacteria are very tedious and labor intense. Fast on-site screening for pathogens based on volatile organic compounds (VOCs) by ion mobility spectrometry (IMS) could help to differentiate between healthy and potentially infected subjects. Read More

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Fine-mapping and candidate gene analysis for the foxglove aphid resistance gene Raso2 from wild soybean PI 366121.

Theor Appl Genet 2021 May 11. Epub 2021 May 11.

Department of Crop Science and Biotechnology, Dankook University, Cheonan, 31116, Korea.

Key Message: The foxglove aphid resistance gene Raso2 from PI 366121 was fine-mapped to 77 Kb region, and one candidate gene was identified. The foxglove aphid (FA: Aulacorthum solani Kaltenbach) is an important insect pest that causes serious yield losses in soybean. The FA resistance gene Raso2 from wild soybean PI 366121 was previously mapped to a 13 cM interval on soybean chromosome 7. Read More

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THE PI3K/Akt/mTOR Signaling Pathway Plays a Role in Regulating Autophagy in Mouse Oocytes During Vitrification-warming and in vitro Maturation.

Cryo Letters 2020 Jan-Feb;41(1):31-37

Hospital for Reproductive Medicine Affiliated to Shandong University, Jinan, China.

Background: It has been shown that vitrified-warmed oocytes exhibit autophagic activation. However, its regulatory molecular mechanism of autophagy needs to be clarified.

Objective: To determine the role of signaling pathway of PI3K/Akt/mTOR in the regulation of autophagy in oocytes during vitrification-warming and IVM. Read More

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Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK-FoxO1-TFEB signalling axis.

J Cell Mol Med 2021 May 10. Epub 2021 May 10.

Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, China.

Abnormal accumulation of lipids and massive deposition of foam cells is a primary event in the pathogenesis of atherosclerosis. Recent studies have demonstrated that autophagy and lysosomal function of atherosclerotic macrophages are impaired, which exacerbates the accumulation of lipid in macrophages and formation of foam cells. Gastrodin, a major active component of Gastrodia elata Bl. Read More

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Lactobacillus plantarum as feed additive to improvement in vitro ruminal biofermentation and digestibility of some tropical tree leaves.

J Appl Microbiol 2021 May 10. Epub 2021 May 10.

Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Estado de México, México.

Aim: This study was performed to investigate the effects of Lactobacillus plantarum (LP) on the in vitro gas production (GP) kinetics and ruminal fermentation parameter of three species of tropical forage Ziziphus mauritiana, Acacia victoria and Moringa oleifera.

Methods And Results: Experiment treatments were 1) Z. mauritiana (Z) without LP (-ZLP); 2) Z. Read More

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Enhanced empirical likelihood estimation of incubation period of COVID-19 by integrating published information.

Stat Med 2021 May 11. Epub 2021 May 11.

Key Laboratory of Advanced Theory and Application in Statistics and Data Science, MOE, Shanghai, China.

Since the outbreak of the new coronavirus disease (COVID-19), a large number of scientific studies and data analysis reports have been published in the International Journal of Medicine and Statistics. Taking the estimation of the incubation period as an example, we propose a low-cost method to integrate external research results and available internal data together. By using empirical likelihood method, we can effectively incorporate summarized information even if it may be derived from a misspecified model. Read More

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The Incubation Period of COVID-19: Current Understanding and Modeling Technique.

Char Leung

Adv Exp Med Biol 2021 ;1318:81-90

Deakin University, Burwood, VIC, Australia.

This chapter aims to answer the following questions regarding the incubation period of COVID-19. Why is understanding the incubation period of COVID-19 important? How long is the incubation time, and what are the associating factors? How should the incubation period be modeled given the current pandemic situation? Where should we go from here? As a critical epidemiological metric, the incubation period is of public health and clinical importance. While the incubation time of COVID-19 is generally similar to that of SARS and MERS, recent studies identifying factors that impact the incubation period of COVID-19, travel history, for example, only tell part of the story. Read More

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January 2021

Divergent Anatomical Correlates and Functional Network Connectivity Patterns in Temporal Lobe Epilepsy with and Without Depression.

Brain Topogr 2021 May 10. Epub 2021 May 10.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Epilepsy and depression were proposed to facilitate each other reciprocally through common neurobiological anomalies, especially the prefrontal-limbic-subcortical abnormalities. Yet neuroimaging patterns of higher-order cognitive networks and neuroanatomical correlates were rarely compared in temporal lobe epilepsy patients with (TLE-D) and without depression (TLE-N). We collected T1-weighted structural and resting-state functional MRI data from 20 TLE-D, 31 TLE-N and 20 healthy controls (HCs) and performed analyses including hippocampal volume (HCV), cortical thickness, gray matter volume (GMV) and whole-brain functional network connectivity (FNC) across three groups. Read More

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A hemagglutination-based, semi-quantitative test for point-of-care determination of SARS-CoV-2 antibody levels.

medRxiv 2021 May 4. Epub 2021 May 4.

Serologic, point-of-care tests to detect antibodies against SARS-CoV-2 are an important tool in the COVID-19 pandemic. The majority of current point-of-care antibody tests developed for SARS-CoV-2 rely on lateral flow assays, but these do not offer quantitative information. To address this, we developed a new method of COVID-19 antibody testing employing hemagglutination tested on a dry card, similar to that which is already available for rapid typing of ABO blood groups. Read More

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An aptasensor using ceria electrodeposited-screen-printed carbon electrode for detection of epithelial sodium channel protein as a hypertension biomarker.

R Soc Open Sci 2021 Feb 17;8(2):202040. Epub 2021 Feb 17.

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia.

Epithelial sodium channel (ENaC) is a transmembrane protein that has an essential role in maintaining the levels of sodium in blood plasma. A person with a family history of hypertension has a high enough amount of ENaC protein in the kidneys or other organs, so that the ENaC protein acts as a marker that a person is susceptible to hypertension. An aptasensor involves aptamers, which are oligonucleotides that function similar to antibodies, as sensing elements. Read More

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February 2021

The importance of ongoing international surveillance for Creutzfeldt-Jakob disease.

Nat Rev Neurol 2021 May 10. Epub 2021 May 10.

National CJD Research & Surveillance Unit, Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, UK.

Creutzfeldt-Jakob disease (CJD) is a rapidly progressive, fatal and transmissible neurodegenerative disease associated with the accumulation of misfolded prion protein in the CNS. International CJD surveillance programmes have been active since the emergence, in the mid-1990s, of variant CJD (vCJD), a disease linked to bovine spongiform encephalopathy. Control measures have now successfully contained bovine spongiform encephalopathy and the incidence of vCJD has declined, leading to questions about the requirement for ongoing surveillance. Read More

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AFM study of changes in properties of horseradish peroxidase after incubation of its solution near a pyramidal structure.

Sci Rep 2021 May 10;11(1):9907. Epub 2021 May 10.

Institute of Biomedical Chemistry, Pogodinskaya str., 10, Moscow, 119121, Russia.

In our present paper, the influence of a pyramidal structure on physicochemical properties of a protein in buffer solution has been studied. The pyramidal structure employed herein was similar to those produced industrially for anechoic chambers. Pyramidal structures are also used as elements of biosensors. Read More

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The combination of dantrolene and nimodipine effectively reduces 5-HT-induced vasospasms in diabetic rats.

Sci Rep 2021 May 10;11(1):9852. Epub 2021 May 10.

Department of Physiology, University of Puerto Rico-School of Medicine, GPO Box 365067, San Juan, PR, 00936-5067, USA.

Diabetics have a higher risk of developing cerebral vasospasms (CVSP) after subarachnoid hemorrhagic stroke than non-diabetics. Serotonin (5-HT) is one of the key vasoconstrictors released in the hemorrhagic blood and an important contributor to the etiology of CVSP. The combination of the ryanodine receptor blocker dantrolene and the Ca2+ channel blocker nimodipine significantly reduces phenylephrine (PHE)-induced vascular contraction in both diabetic and nondiabetic rats, but the effectiveness of this drug combination in reducing 5-HT-induced contraction is unknown. Read More

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Pharmacokinetics and antifungal activity of echinocandins in ascites fluid of critically ill patients.

Antimicrob Agents Chemother 2021 May 10. Epub 2021 May 10.

Clinical Pharmacokinetics Unit, Division of Medical Intensive Care and Emergency Medicine, Department of Internal Medicine I, Medical University of Innsbruck, Innsbruck, Austria

Pharmacokinetics and antifungal activity of the echinocandins anidulafungin (AFG), micafungin (MFG) and caspofungin (CAS) were assessed in ascites fluid and plasma of critically ill adults treated for suspected or proven invasive candidiasis. Ascites fluid was obtained from ascites drains or during paracentesis. The antifungal activity of the echinocandins in ascites fluid was assessed by incubation of and at concentrations of 0. Read More

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LPS decreases CFTR open probability and mucociliary transport through generation of reactive oxygen species.

Redox Biol 2021 Apr 30;43:101998. Epub 2021 Apr 30.

Department of Otolaryngology Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, AL, USA; Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address:

Lipopolysaccharide (LPS) serves as the interface between gram-negative bacteria (GNB) and the innate immune response in respiratory epithelial cells (REC). Herein, we describe a novel biological role of LPS that permits GNB to persist in the respiratory tract through inducing CFTR and mucociliary dysfunction. LPS reduced cystic fibrosis transmembrane conductance regulater (CFTR)-mediated short-circuit current in mammalian REC in Ussing chambers and nearly abrogated CFTR single channel activity (defined as forskolin-activated Cl currents) in patch clamp studies, effects of which were blocked with toll-like receptor (TLR)-4 inhibitor. Read More

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Novel approach for Monte Carlo simulation of the new COVID-19 spread dynamics.

Infect Genet Evol 2021 May 7:104896. Epub 2021 May 7.

National Technical University of Athens, Physics Department, Greece.

A Monte Carlo simulation in a novel approach is used for studying the problem of the outbreak and spread dynamics of the new COVID-19 pandemic in this work. In particular, our goal was to generate epidemiological data based on natural mechanism of transmission of this disease assuming random interactions of a large-finite number of individuals in very short distance ranges. In the simulation we also take into account the stochastic character of the individuals in a finite population and given densities of people. Read More

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Static and dynamic culture of human endothelial cells encapsulated inside alginate-gelatin microspheres.

Microvasc Res 2021 May 7:104174. Epub 2021 May 7.

Maragheh University of Medical Sciences, Maragheh, Iran.

This study aimed to explore the angiogenesis potential of human endothelial cells encapsulated inside alginate-gelatin microspheres under static and dynamic culture systems after 7 days. Human umbilical vein endothelial cells were encapsulated inside alginate (1%) and gelatin (1.2%) using an electrostatic encapsulation method. Read More

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Production of chemotherapeutic enzyme L-asparaginase from fungal source.

Curr Pharm Biotechnol 2021 Feb 11. Epub 2021 Feb 11.

Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749. Korea.

Background: L-Asparaginase is an antineoplastic agent used in the treatment of acute myeloid and acute lymphoblastic leukemia. The present study deals with the production of this chemotherapeutic enzyme drug from Aspergillus flavus NCIM 526. The production of enzymes was carried out using oil-extracted cakes in shake flask culture. Read More

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February 2021

Derivation and characterization of putative embryonic stem cells isolated from blastoderms of Taiwan Country chicken for the production of chimeric chickens.

Anim Biotechnol 2020 Dec 2:1-10. Epub 2020 Dec 2.

Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.

The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed's adaptability to the area and disease resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regenerative medicine and species conservation, the aims of this study were to isolate and characterize ESCs of TCC. Read More

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December 2020

Biocatalytic asymmetric synthesis of (R)-1-tetralol using Lactobacillus paracasei BD101.

Chirality 2021 May 10. Epub 2021 May 10.

Faculty of Health Sciences, Department of Nutrition and Dietetics, Bayburt University, Bayburt, Turkey.

Asymmetric bioreduction of ketones is a fundamental process in the production of organic molecules. Compounds containing tetralone rings are found in the structure of many biologically active and pharmaceutical molecules. Biocatalytic reduction of ketones is one of the most promising and significant routes to prepare optically active alcohols. Read More

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First Report of Causing Target Spot on Soybean in Taiwan.

Plant Dis 2021 May 10. Epub 2021 May 10.

Michigan State University, Plant, Soil and Microbial Sciences, 578 Wilson Rd, CIPS104, East Lansing, United States, 48824;

Soybean ( [L.] Merr.) is an important crop in Taiwan. Read More

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J Orthop Res 2021 May 10. Epub 2021 May 10.

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.

Meniscus allograft transplantations (MAT) represent established surgical procedures with proven outcomes. Yet, storage as frozen specimens and limited cellular repopulation may impair graft viability. This proof of concept study tests the feasibility of injecting allogeneic mesenchymal stromal/stem cells (MSCs) in meniscus allograft tissue. Read More

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The interplay between HIF-1α and long noncoding GAS5 regulates the JAK1/STAT3 signalling pathway in hypoxia-induced injury in myocardial cells.

Cardiovasc Diagn Ther 2021 Apr;11(2):422-434

Liaoning Jinzhou Inspection and Testing Certification Center, Jinzhou, China.

Background: Long non-coding RNA (lncRNA) GAS5 is associated with hypoxia-induced diseases whereas hypoxia-inducible factor-1α (HIF-1α) plays an important role in hypoxic injury of cells. The current study explores the regulatory functions of GAS5/HIF-1α which co-play in anoxic injury among rat cardiomyocytes H9C2 cells.

Methods: Hypoxia model was established through anoxic incubation while normal culture of H9C2 cells was considered as control. Read More

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Secreted frizzled-related protein 4 promotes brown adipocyte differentiation.

Exp Ther Med 2021 Jun 15;21(6):637. Epub 2021 Apr 15.

Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. China.

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of frizzled proteins. In the present report, the effects of SFRP4 on murine brown adipocyte differentiation were evaluated, which exhibited an intrinsic capacity to differentiate with high efficiency. Brown preadipocytes were isolated from the scapular region of brown adipose tissue, which showed that the overexpression of recombinant active SFRP4 protein at three concentrations (1, 10 and 100 ng/ml) significantly increased the expression of adipocyte differentiation-associated genes (C/EBPα, C/EBPβ, UCP-1, PRDM16, PGC1α and GLUT4) in a dose-dependent manner compared with the control group. Read More

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Regulates Differentiation of Infection Structures Induced by Physicochemical Signals From Pear Fruit Cuticular Wax, Secondary Metabolism, and Pathogenicity of .

Front Plant Sci 2021 21;12:642601. Epub 2021 Apr 21.

College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.

, the casual agent of black rot of pear fruit, can sense and respond to the physicochemical cues from the host surface and form infection structures during infection. To evaluate the role of cyclic AMP-dependent protein kinase (cAMP-PKA) signaling in surface sensing of , we isolated and functionally characterized the cyclic adenosine monophosphate-dependent protein kinase A catalytic subunit gene (). Gene expression results showed that was strongly expressed during the early stages of appressorium formation on hydrophobic surfaces. Read More

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Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of , and Their Ability to Bind Aflatoxin B, Ochratoxin A, and Zearalenone .

Front Microbiol 2021 22;12:655386. Epub 2021 Apr 22.

Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.

The increased consumption of plant-based foods has intensified the concern related to mycotoxin intoxication. This study aimed to investigate the effect of selected lactic acid bacteria (LAB) strains on the growth of NRRL 2999 and its production of aflatoxin (AF). The ability of the heat-killed (100°C for 1 h) LAB strains to bind aflatoxin M (AFM) in milk and aflatoxin B (AFB), ochratoxin A (OTA), and zearalenone (ZEN) in potassium phosphate buffer (PPB) was also evaluated . Read More

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Glycated Albumin Triggers an Inflammatory Response in the Human Airway Epithelium and Causes an Increase in Ciliary Beat Frequency.

Front Physiol 2021 23;12:653177. Epub 2021 Apr 23.

Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile.

The role of inflammation in airway epithelial cells and its regulation are important in several respiratory diseases. When disease is present, the barrier between the pulmonary circulation and the airway epithelium is damaged, allowing serum proteins to enter the airways. We identified that human glycated albumin (GA) is a molecule in human serum that triggers an inflammatory response in human airway epithelial cultures. Read More

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