Int J Biol Macromol 2021 Jul 20;187:1-8. Epub 2021 Jul 20.
College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China; Sugar Industry Collaborative Innovation Center, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China. Electronic address:
The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered Bacillus subtilis. Then, the 5'-untranslated region (5'-UTR) structure of the mRNA translational initiation region was optimized for the precise control of DPEase expression. Read More