7,452 results match your criteria including 230


Patient perspective on telemedicine replacing physical consultations in urology during the COVID-19 lockdown in Denmark.

Scand J Urol 2021 May 11:1-7. Epub 2021 May 11.

Department of Urology, Odense University Hospital, Odense, Denmark.

Objective: In March-April 2020, during the coronavirus disease 2019 (COVID-19) pandemic lockdown in Denmark, the Danish Health Authorities recommended that, where possible, face-to-face patient-physician consultations be replaced by telephone consultations. The aim of this study was to obtain patients' evaluation of their telemedicine experience.

Methods: Patients who were candidates for telemedicine consultations were recruited based on their urological ailment, necessity for follow-up and comorbidity. Read More

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Spray-induced gene silencing as a potential tool to control potato late blight disease.

Phytopathology 2021 May 11. Epub 2021 May 11.

Swedish University of Agricultural Sciences , Plant Protection Biologu, Växtskyddsvägen 1, Box 102, Horticum, Alnarp, Sweden, SE-230 53;

Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target P. infestans genes that are essential for infection. Read More

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The complete chloroplast genome of : molecular structures and comparative analysis.

Mitochondrial DNA B Resour 2021 Apr 19;6(4):1431-1439. Epub 2021 Apr 19.

Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

is a common ornamental arbor in the genus (family Rosaceae). Here, we sequenced and assembled the complete plastome of using the next-generation DNA sequencing technology. And we then compared it with nine species using a range of bioinformatics software tools. Read More

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Plasma Metabolites Alert Patients With Chest Pain to Occurrence of Myocardial Infarction.

Front Cardiovasc Med 2021 23;8:652746. Epub 2021 Apr 23.

Laboratory of Metabolomics, Jiangsu Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.

Myocardial infarction (MI) is one of the leading causes of death worldwide, and knowing the early warning signs of MI is lifesaving. To expand our knowledge of MI, we analyzed plasma metabolites in MI and non-MI chest pain cases to identify markers for alerting about MI occurrence based on metabolomics. A total of 230 volunteers were recruited, consisting of 146 chest pain patients admitted with suspected MI (85 MIs and 61 non-MI chest pain cases) and 84 control individuals. Read More

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Genome-wide identification, characterization and expression profiles of the gene family in species.

3 Biotech 2021 May 1;11(5):249. Epub 2021 May 1.

State Key Laboratory of Cotton Biology, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng, China.

Carotenoid cleavage dioxygenases (CCDs) are a group of enzymes that catalyze the selective oxidative cleavage steps from carotenoids to apocarotenoids, which are essential for the synthesis of biologically important molecules such as retinoids, and the phytohormones abscisic acid (ABA) and strigolactones. In addition, CCDs play important roles in plant biotic and abiotic stress responses. Till now, a comprehensive characterization of the gene family in the economically important crop cotton ( spp. Read More

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Experimental design optimization of simultaneous enantiomeric separation of atenolol and chlorthalidone binary mixture by high-performance liquid chromatography using polysaccharide-based stationary phases.

Chirality 2021 May 8. Epub 2021 May 8.

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

In this work, enantiomeric separation of a drug combination of two chiral drugs, namely, atenolol and chlorthalidone, is described. Prior investigation of the effect of different variables on the resolution of the enantiomers' peaks and the total run time represented by the retention time of the last eluted peak was conducted using face-centered composite design. Twenty-two experiments were carried out by varying the chiral stationary phase type as a categorical factor and mobile phase composition including the percentage of ethanol and percentage of diethylamine as continuous factors. Read More

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Draft nuclear genome and complete mitogenome of the Mediterranean corn borer, Sesamia nonagrioides, a major pest of maize.

G3 (Bethesda) 2021 May 7. Epub 2021 May 7.

Université Paris-Saclay, CNRS, IRD, UMR Évolution, Génomes, Comportement et Écologie, 91198, Gif-sur-Yvette, France.

The Mediterranean corn borer (Sesamia nonagrioides, Noctuidae, Lepidoptera) is a major pest of maize in Europe and Africa. Here, we report an assembly of the nuclear and mitochondrial genome of a pool of inbred males and females third instar larvae, based on short- and long-read sequencing. The complete mitochondrial genome is 15,330 bp and contains all expected 13 and 24 protein-coding and RNA genes, respectively. Read More

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Japanese carotenoid database with α- and β-carotene, β-cryptoxanthin, lutein, zeaxanthin, lycopene, and fucoxanthin and intake in adult women.

Int J Vitam Nutr Res 2021 May 7:1-12. Epub 2021 May 7.

Ochanomizu University, Tokyo, Japan.

Carotenoid intake is associated with low mortality and cancer risks; data on non-provitamin carotenoid intake is limited especially in Asians. We aimed to estimate carotenoid intake in Japanese adult women. Carotenoid content database comprises 196 food items, including 39 fruits, 87 vegetables and mushrooms, and 11 seaweeds, and was established using data from the literature and analyses of foods available in Japan. Read More

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Effectiveness of Pfizer-BioNTech and Moderna Vaccines Against COVID-19 Among Hospitalized Adults Aged ≥65 Years - United States, January-March 2021.

MMWR Morb Mortal Wkly Rep 2021 May 7;70(18):674-679. Epub 2021 May 7.

Adults aged ≥65 years are at increased risk for severe outcomes from COVID-19 and were identified as a priority group to receive the first COVID-19 vaccines approved for use under an Emergency Use Authorization (EUA) in the United States (1-3). In an evaluation at 24 hospitals in 14 states,* the effectiveness of partial or full vaccination with Pfizer-BioNTech or Moderna vaccines against COVID-19-associated hospitalization was assessed among adults aged ≥65 years. Among 417 hospitalized adults aged ≥65 years (including 187 case-patients and 230 controls), the median age was 73 years, 48% were female, 73% were non-Hispanic White, 17% were non-Hispanic Black, 6% were Hispanic, and 4% lived in a long-term care facility. Read More

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Percutaneous endovascular abdominal aortic aneurysm repair with monitored anesthesia care decreases operative time but not pulmonary complications.

Vascular 2021 May 3:17085381211012908. Epub 2021 May 3.

Vascular & Endovascular Surgery, University of Miami & Miami VAMC, Miami, FL, USA.

Objectives: To report our experience and compare the results of percutaneous endovascular aortic aneurysm repair (PEVAR) performed under monitored anesthesia care (MAC) to PEVAR under general anesthesia (GA).

Methods: A retrospective review of patients who underwent non-emergency endovascular abdominal aortic aneurysm repair (EVAR) was completed. Patients were excluded if they had a complex repair, including fenestrated, branched, or parallel endografting. Read More

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Chemical profiling and multi-components quantitative analysis of Panzerina lanata by ultra-fast liquid chromatography with tandem mass spectrometry.

J Sep Sci 2021 May 3. Epub 2021 May 3.

School of Pharmacy, Key Laboratory of Ethnomedicine of Ministry of Education, Minzu University of China, Beijing, China.

Panzerina lanata is a Chinese medicine with the bioactivity of detumescence and detoxification. In this study, novel qualitative and quantitative methods were established by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry and ultra-high-performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry, respectively. As a result, 20 compounds were identified or tentatively characterized, including flavonoids, organic acids, alkaloids, and lignans, 5 of which were identified for the first time based on the reference standards. Read More

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A comparison of intravenous methylprednisolone and hydrocortisone for the treatment of acute inflammatory bowel disease.

J Gastroenterol Hepatol 2021 May 3. Epub 2021 May 3.

Department of Gastroenterology, North Shore Hospital, Auckland, New Zealand.

Background And Aim: Despite widespread recommendations and use of intravenous corticosteroids (IVCS) for the treatment of acute flares of ulcerative colitis and Crohn's disease, limited evidence exists comparing outcomes of the two most common regimens, intravenous methylprednisolone (IVMP) and intravenous hydrocortisone (IVHC). IVHC has stronger mineralocorticoid effects compared with IVMP and may cause higher rates of hypokalemia. We aimed to determine differences in clinical outcomes including requirement for inpatient rescue therapy, bowel resection, and rates of hypokalemia. Read More

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Deaths and Severe Adverse Events after the use of Mifepristone as an Abortifacient from September 2000 to February 2019.

Issues Law Med 2021 ;36(1):3-26

Associate Professor of Family Medicine, Liberty University College of Osteopathic Medicine.

Objectives: Primary: Analyze the Adverse Events (AEs) reported to the Food and Drug Administration (FDA) after use of mifepristone as an abortifacient. Secondary: Analyze maternal intent after ongoing pregnancy and investigate hemorrhage after mifepristone alone.

Methods: Adverse Event Reports (AERs) for mifepristone used as an abortifacient, submitted to the FDA from September 2000 to February 2019, were analyzed using the National Cancer Institute's Common Terminology Criteria for Adverse Events (CTCAEv3). Read More

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January 2021

Targeted dual inhibition of c-Met/VEGFR2 signalling by foretinib improves antitumour effects of nanoparticle paclitaxel in gastric cancer models.

J Cell Mol Med 2021 May 3. Epub 2021 May 3.

Department of Surgery, Indiana University School of Medicine, South Bend, IN, USA.

Elevated expression of multiple growth factors and receptors including c-Met and VEGFR has been reported in gastric adenocarcinoma (GAC) and thus provides a potentially useful therapeutic target. The therapeutic efficacy of foretinib, a c-Met/VEGFR2 inhibitor, was determined in combination with nanoparticle paclitaxel (NPT) in GAC. Animal studies were conducted in NOD/SCID mice in subcutaneous and peritoneal dissemination xenografts. Read More

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A Novel Clinical Nomogram to Predict Transient Symptomatic Associated with Infarction: The ABCD3-SLOPE Score.

Biomed Res Int 2021 14;2021:5597155. Epub 2021 Apr 14.

Educational Office, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Background: It is hard to differentiate transient symptoms associated with infarction (TSI) from transient ischemic stroke (TIA) without MRI in the early onset. However, they have distinct clinical outcomes and respond differently to therapeutics. Therefore, we aimed to develop a risk prediction model based on the clinical features to identify TSI. Read More

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Preoperative Prediction of Gartland IV Supracondylar Fractures of Humerus: Is it Possible?

Rev Bras Ortop (Sao Paulo) 2021 Apr 31;56(2):230-234. Epub 2021 Mar 31.

Departamento de Ortopedia, All India Institute of Medical Sciences, Rishikesh, Índia.

 The present study aims to identify preoperative characteristics of the patient, of the injury, as well as of imaging, which would point towards a type IV fracture. The present study shall help the operating team to predict more accurately the type IV pattern preoperatively, leading to improved counselling of the caregivers, planning of surgery, as well as preparedness regarding open reduction, if such situation arises.  A retrospective study was conducted, including patients that met the following criteria: 1) age < 16 years old; 2) Gartland type-III and type-IV supracondylar fractures; and 3) with complete records. Read More

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Analysis of C9orf72 Intermediate Alleles in a Retrospective Cohort of Neurological Patients: Risk Factors for Alzheimer's Disease?

J Alzheimers Dis 2021 Apr 28. Epub 2021 Apr 28.

Fondazione IRCCS Ca' Granda, Ospedale Policlinico, Milan, Italy.

Background: C9orf72 hexanucleotide GGGGCC (G4C2) large repeat expansions within the first intron of the gene are a major cause of familial frontotemporal dementia, but also of apparently sporadic cases. Alleles with >  30 repeats are often considered pathogenic, but the repeat length threshold is still undefined. It is also unclear if C9orf72 intermediate alleles (9-30 repeats) have clinically significant effects. Read More

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Label free structure-switching fluorescence polarization detection of chloramphenicol with truncated aptamer.

Talanta 2021 Aug 23;230:122349. Epub 2021 Mar 23.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China; Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi, 214122, China. Electronic address:

In this study, the original chloramphenicol aptamer containing 80 bases was truncated to 30 bases with high affinity by the SYBR Green I assay. It was found that the ionic strength and type affect the recognition of aptamers, especially magnesium ion played a vital role in the binding process. Furthermore, the binding performance of aptamer, including binding mode, key binding sites and conformational changes were further investigated by circular dichroism spectroscopy, UV-vis absorption spectrum and molecular docking. Read More

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Quantitative quadrupolar NMR (qQNMR) using nitrogen-14 for the determination of choline in complex matrixes.

Talanta 2021 Aug 23;230:122344. Epub 2021 Mar 23.

Department of Chemistry and Physics, Research Centre CIAIMBITAL, University of Almería, Ctra. Sacramento, s/n, 04120, Almería, Spain. Electronic address:

NMR offers the unique potential to selectively excite the chosen nuclei avoiding in an extraordinary way the matrix effect. Quantitative Nitrogen-14 NMR (N qNMR) spectroscopy has been introduced for the first time as a robust and validated method to determine choline in a variety of matrixes including quinoa grains, instant coffee and food supplements. A study about the ion pairing of choline bitartrate in aqueous solution by means of diffusion PGSE, NOESY and HOESY NMR have been also provided. Read More

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Selective analysis of interferon-alpha in human serum with boronate affinity oriented imprinting based plastic antibody.

Authors:
Zijun Bie Yang Chen

Talanta 2021 Aug 23;230:122338. Epub 2021 Mar 23.

Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China; School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233000, China. Electronic address:

Interferons are important biomolecules in human immune system. Cytokine interferon alpha (IFN-α), a type I interferon, is one of the major components of the innate immune response involved in autoimmune diseases. Thus, the analysis of interferons is of great importance for both biological and pharmaceutical purposes. Read More

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Handheld laser-induced fluorescence detection systems with different optical configurations.

Talanta 2021 Aug 23;230:122329. Epub 2021 Mar 23.

Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou, 310007, China; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Electronic address:

There is a growing urgent requirement for miniaturized laser-induced fluorescence (LIF) detection systems in many research fields. In this work, miniaturized LIF detectors with three different optical configurations of orthogonal, confocal, and oblique were developed, using a laser diode as the excitation source and a photodiode as the photodetector. The computer simulation and experimental methods were used to investigate the distributions of laser scattered light and fluorescent light near the detection window. Read More

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A rapid and sensitive method for characterization and quantification of polyglycerol esters by supercritical fluid chromatography coupled to high-resolution mass spectrometry (SFC-HRMS).

Talanta 2021 Aug 24;230:122316. Epub 2021 Mar 24.

Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 91198, Gif-sur-Yvette, France. Electronic address:

Polyglycerol esters of fatty acids (PGEs), a very complex mixture of various isomers, are widely used as green surfactants in different industrial fields such as in cosmetic, pharmaceutic and food industries. However, no study related to the purification and the absolute quantification of these compounds has been described yet. In this study, we developed a rapid and efficient method for characterization and quantification of PGEs using Supercritical Fluid CO coupled to High-Resolution Mass Spectrometry (SFC-HRMS). Read More

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A novel vortex assisted dispersive solid phase extraction of some trace elements in essential oils and fish oil.

Talanta 2021 Aug 17;230:122312. Epub 2021 Mar 17.

Chemistry Department, Faculty of Science and Arts, BalıkesirUniversity, Ça ğış Campus, Balıkesir, 10145, Turkey. Electronic address:

In this paper, a novel vortex assisted solid phase extraction procedure combined with inductively coupled plasma optic emission spectrometry was presented for determination of Mn, Cu, Ni, Cr, Cd and Pb directly in oily matrix. The suggested separation and preconcentration method was based on simultaneous sorption of the target analytes from real essential oil matrix onto γ-AlO functionalized with fluorescein (Al-Flr) and transfer to aqueous eluent prior to quantification. The effect of different parameters including sorption and elution time, sample volume, eluent type and concentration and interfering ions were investigated. Read More

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Metal-coded hydrogel magnetic molecularly imprinted polymer for preconcentration and cleanup of sarcosine: Determination in urine; coupled to on-column capillary electrophoresis.

Talanta 2021 Aug 20;230:122309. Epub 2021 Mar 20.

Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

In this study, sarcosine metal-coded hydrogel magnetic molecularly imprinted polymer (Hydro-MeC-MMIP) has been fabricated and coupled to on-column derivatization capillary electrophoresis (CE). As a metal-coding approach, sarcosine-Cu-ligand (Sar-Cu-L) chelate complex was introduced as a template to overcome the problems associated with the fabrication of MMIP for a small molecule having limited functional groups such as sarcosine. To our best knowledge, it is the first time that methacrylamide (MA) coated FeO (FeO@MA) with abounded reactive double-bound on the surface has been used as a magnetic core in the one-pot synthesis of MMIPs. Read More

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Construction of a ternary nano-architecture based graphene oxide sheets, toward electrocatalytic determination of tumor-associated anti-p53 autoantibodies in human serum.

Talanta 2021 Aug 19;230:122276. Epub 2021 Mar 19.

Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Almost 13% of all death in the world is related to cancer. One of the major reasons for failing cancer treatment is the late diagnosis of the tumors. Thus, diagnosis at the early stages could be vital for the treatment. Read More

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Effectof Myd88 deficiency on gene expression profiling in salivary glands of female non-obese diabetic (NOD) mice.

J Oral Biosci 2021 Apr 29. Epub 2021 Apr 29.

Department of Oral Microbiology, Division of Oral Infection Health Sciences, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu 501-0296, Japan. Electronic address:

Objectives: Sjögren's syndrome (SS) is a chronic autoimmune disease characterized by inflammatory lesions in the salivary and lacrimal glands, which are caused by distinct lymphocytic infiltrates. Female non-obese diabetic (NOD) mice spontaneously develop inflammatory lesions of the salivary glands with SS-like pathological features. Previous studies have shown that MyD88, a crucial adaptor protein that activates innate immune signaling, affects lymphocytic infiltration, but its detailed role remains unclear. Read More

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Association between Compliance with Movement Behavior Guidelines and Obesity among Malaysian Preschoolers.

Int J Environ Res Public Health 2021 Apr 27;18(9). Epub 2021 Apr 27.

Center for Community Health Studies (ReaCH), Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.

A daily balance of physical activities, sedentary behaviors and sleep are important for maintaining the health of young children. The aim of this study is to explore the association between 24-h movement behavior of Malaysian children aged 4 to 6 years with weight status. A total of 230 preschoolers were recruited from 22 kindergartens in Kuala Lumpur. Read More

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Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon.

Viruses 2021 04 16;13(4). Epub 2021 Apr 16.

Departamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-000, Brazil.

Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples ( = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapá. Read More

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Development of a Robust High-Throughput Screening Platform for Inhibitors of the Striatal-Enriched Tyrosine Phosphatase (STEP).

Int J Mol Sci 2021 Apr 23;22(9). Epub 2021 Apr 23.

Sanford Burnham Prebys Medical Discovery Institute, NCI-Designated Cancer Center, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA.

Many human diseases are the result of abnormal expression or activation of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). Not surprisingly, more than 30 tyrosine kinase inhibitors (TKIs) are currently in clinical use and provide unique treatment options for many patients. PTPs on the other hand have long been regarded as "undruggable" and only recently have gained increased attention in drug discovery. Read More

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Synthesis of Polystyrene-Based Cationic Nanomaterials with Pro-Oxidant Cytotoxic Activity on Etoposide-Resistant Neuroblastoma Cells.

Nanomaterials (Basel) 2021 Apr 10;11(4). Epub 2021 Apr 10.

Department of Experimental Medicine (DIMES), University of Genova, Via Alberti L.B., 16132 Genoa, Italy.

Drug resistance is a multifactorial phenomenon that limits the action of antibiotics and chemotherapeutics. Therefore, it is essential to develop new therapeutic strategies capable of inducing cytotoxic effects circumventing chemoresistance. In this regard, the employment of natural and synthetic cationic peptides and polymers has given satisfactory results both in microbiology, as antibacterial agents, but also in the oncological field, resulting in effective treatment against several tumors, including neuroblastoma (NB). Read More

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