2,433,556 results match your criteria including

LncRNA DLGAP1-AS1 accelerates glioblastoma cell proliferation through targeting miR-515-5p/ROCK1/NFE2L1 axis and activating Wnt signaling pathway.

Brain Behav 2021 Sep 18:e2321. Epub 2021 Sep 18.

Department of Pediatric Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Introduction: Glioblastoma (GBM), the primary malignant tumor in the central nervous system, features high aggressiveness and mortality. Long noncoding RNAs (lncRNAs) can exert the crucial function in regulating various human diseases, including GBM. However, the function and mechanism of lncRNA DLGAP1 antisense RNA 1 (DLGAP1-AS1) in GBM remain still unknown. Read More

View Article and Full-Text PDF
September 2021

Direct immunofluorescence analysis of oral Tzanck smears for pemphigus vulgaris: A diagnostic test.

J Oral Pathol Med 2021 Sep 18. Epub 2021 Sep 18.

Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, China.

Background: Pemphigus vulgaris (PV) is a rare and potentially fatal autoimmune blistering disease. Direct immunofluorescence (DIF) is an important method for PV diagnosis, but oral tissue biopsy for DIF analysis is difficult to perform because of the fragile characteristics of oral mucosa. However, no well-designed diagnostic studies addressing the validity of DIF analysis of oral Tzanck smears for the diagnosis of PV exist. Read More

View Article and Full-Text PDF
September 2021

The Use of Radiomic Analysis of Magnetic Resonance Imaging in Predicting Distant Metastases of Rectal Carcinoma following Surgical Resection - A Systematic Review & Meta-Analysis.

Colorectal Dis 2021 Sep 18. Epub 2021 Sep 18.

Discipline of Surgery, Galway University Hospitals, Galway, H91 YR71, Ireland.

Background: Estimating prognosis in rectal carcinoma (RC) is challenging, with distant recurrence (DR) occurring in up to 30% of cases. Radiomics is a novel field using diagnostic imaging to investigate the tumour heterogeneity of cancers and may have the potential to predict DR.

Aims: To perform a systematic review of current literature evaluating the use of radiomics in predicting DR in patients with resected RC. Read More

View Article and Full-Text PDF
September 2021

Can zirconia crowns be the first restorative choice after endodontic treatment of primary teeth?

Int J Clin Pract 2021 Sep 18:e14888. Epub 2021 Sep 18.

Department of Pediatric Dentistry, Faculty of Dentistry, Erciyes University, Kayseri, Turkey.

Objective: Early loss of primary teeth due to caries can cause temporary or permanent problems, such as occlusion disorders, aesthetic problems, nutritional problems, abnormal habits, and speech disorders. Although the development of restorative materials has led to significant improvements, failures are still frequent. The purpose of this study was to evaluate the success rates of zirconia crown with compare to other crowns for the restoration of endodonticly treated teeth with excessive loss of material. Read More

View Article and Full-Text PDF
September 2021

Recent progress of electrocatalysts for oxygen reduction in fuel cells.

J Colloid Interface Sci 2021 Sep 6;607(Pt 1):791-815. Epub 2021 Sep 6.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, P. R. China. Electronic address:

Oxygen reduction reaction (ORR) has gradually been in the limelight in recent years because of its great application potential for fuel cells and rechargeable metal-air batteries. Therefore, significant issues are increasingly focused on developing effective and economical ORR electrocatalysts. This review begins with the reaction mechanisms and theoretical calculations of ORR in acidic and alkaline media. Read More

View Article and Full-Text PDF
September 2021

Synthesis and pharmacological evaluation of choroquine derivatives bearing long aminated side chains as antivirus and anti-inflammatory agents.

Bioorg Chem 2021 Sep 11;116:105346. Epub 2021 Sep 11.

School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023 China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica (SIMM), Chinese Academy of Sciences, Shanghai 201203, China; Pharm-X Center, School of Pharmaceutical Sciences, Shanghai Jiao Tong University, Shanghai 200240, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address:

Starting from the antimalarial drugs chloroquine and hydroxychloroquine, we conducted a structural optimization on the side chain of chloroquine by introducing amino substituted longer chains thus leading to a series of novel aminochloroquine derivatives. Anti-infectious effects against SARS-Cov2 spike glycoprotein as well as immunosuppressive and anti-inflammatory activities of the new compounds were evaluated. Distinguished immunosuppressive activities on the responses of T cell, B cell and macrophages upon mitogen and pathogenic signaling were manifested. Read More

View Article and Full-Text PDF
September 2021

Complications and prognosis associated with intra-tympanic steroid injection to treat sudden sensorineural hearing impairment.

Am J Otolaryngol 2021 Sep 10;43(1):103221. Epub 2021 Sep 10.

Division of Otology, Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan; School of Medicine, Chang Gung University, Taiwan. Electronic address:

Purpose: The purpose of this study was to measure the incidence of complications in sudden sensorineural hearing loss (SSNHL) patients treated with intra-tympanic steroid injection (ITSI) and compare hearing recovery rates.

Materials And Methods: 123 patients with unilateral SSNHL receiving ITSIs were included in this study. Post-ITSI complications were documented including otalgia, dysgeusia, vertigo (duration>1 h), and persistent eardrum perforation. Read More

View Article and Full-Text PDF
September 2021

Primary external auditory canal meningioma: Case report and review of the literature.

Am J Otolaryngol 2021 Sep 11;43(1):103215. Epub 2021 Sep 11.

Puget Sound Ear, Nose and Throat, 21911 76th Ave W, Edmonds, WA, 98026, United States of America.

Meningiomas are tumors that arise from arachnoid cells attached to both the pia mater and the inner portion of the arachnoid. They are common intracranial tumors, representing 12-25% of intracranial neoplasms. Intracranial meningiomas can spread extracranially to involve surrounding structures, including the ear and temporal bone. Read More

View Article and Full-Text PDF
September 2021

New onset tinnitus in the absence of hearing changes following COVID-19 infection.

Am J Otolaryngol 2021 Sep 10;43(1):103208. Epub 2021 Sep 10.

Department of Otolaryngology-Head and Neck Surgery, Mayo Clinic, Rochester, MN, United States of America. Electronic address:

Background: A variety of neurosensory symptoms including tinnitus have been associated with COVID-19 infection. While most cases of tinnitus are associated with hearing loss, here we report a case of severe tinnitus following COVID-19 infection with normal thresholds through 8000 Hz.

Case Report: A 49-year-old male presented with new onset severe tinnitus following COVID-19 infection. Read More

View Article and Full-Text PDF
September 2021

Evaluation of the impact of deep neuromuscular blockade on surgical conditions for laryngeal microsurgery with High Frequency Jet Ventilation. A comparison with no block during intravenous general anesthesia with topical lidocaine.

Am J Otolaryngol 2021 Sep 4;43(1):103187. Epub 2021 Sep 4.

Université catholique de Louvain, CHU UCL Namur, Department of Anesthesiology, Avenue Dr G Thérasse 1, 5530 Yvoir, Belgium.

Objective: Laryngeal transoral surgery classically requires a neuromuscular block (NMB) to facilitate tracheal intubation and to improve surgical conditions. However, the short duration of most procedures and the potential complications of residual NMB lead to consider a no block approach. The hypothesis that intravenous anesthesia (remifentanil and propofol infusions) without NMB but including glottis topical lidocaine anesthesia would allow clinically acceptable laryngeal exposure and good surgical conditions was tested in the specific context of procedures undergone with High Frequency Jet Ventilation (HFJV). Read More

View Article and Full-Text PDF
September 2021

Whole-genome analysis of SARS-CoV-2 samples indicate no tissue specific genetic adaptation of the virus in COVID-19 patients' upper and lower respiratory tract.

Diagn Microbiol Infect Dis 2021 Aug 20;101(4):115520. Epub 2021 Aug 20.

Department of General Pathology and Molecular Diagnostics, University of Augsburg, Augsburg, Germany.

Sample panels of SARS-CoV-2 cases were retrospectively whole-genome sequenced. In three individuals, samples of upper and lower respiratory tract resulted in identical sequences suggesting virus stability including the spike protein cleavage site. In a fourth case, low-level intra-host genomic evolution and a unique 5-nucleotide deletion was observed. Read More

View Article and Full-Text PDF

Stable carbon isotopic characteristics of fossil fuels in China.

Sci Total Environ 2021 Sep 10;805:150240. Epub 2021 Sep 10.

State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an 710061, China; Shaanxi Key Laboratory of Accelerator Mass Spectrometry Technology and Application, Xi'an AMS Center, Xi'an 710061, China.

Good knowledge on the stable carbon isotopic composition (δC) of fossil fuels is critical for the estimation of atmospheric CO sources. Here, we complied a comprehensive δC database including 336 coal, 580 oil, and 1160 natural gas data based on the extensive literature search, and conducted field measurements in two megacities, to characterize the δC signatures of Chinese fossil fuels. Results show that coal exhibits a narrow range and the most enriched in δC signature, oil displays intermediate variations both in the distribution and value of δC. Read More

View Article and Full-Text PDF
September 2021

Concentrations, spatial distribution, sources and environmental health risks of potentially toxic elements in urban road dust across China.

Sci Total Environ 2021 Sep 10;805:150266. Epub 2021 Sep 10.

Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Potentially toxic element (PTE) pollution is widespread in road dust across China, and the effects of PTEs in road dust on health cannot be ignored. In this study, the concentrations of six PTEs (Pb, Cd, Cr, Cu, Zn and Ni) in 4336 road dust samples from 58 cities in 31 provincial regions of China taken after 2000 were obtained from the literatures. Based on these data, the spatial distribution, pollution sources, and ecological and human health risks of PTEs in road dust were comprehensively assessed and the main pollution factors and areas of high risk were identified. Read More

View Article and Full-Text PDF
September 2021

Artificial intelligence-based hybrid deep learning models for image classification: The first narrative review.

Comput Biol Med 2021 Aug 27;137:104803. Epub 2021 Aug 27.

Stroke Diagnosis and Monitoring Division, AtheroPoint™, Roseville, CA, USA. Electronic address:

Background: Artificial intelligence (AI) has served humanity in many applications since its inception. Currently, it dominates the imaging field-in particular, image classification. The task of image classification became much easier with machine learning (ML) and subsequently got automated and more accurate by using deep learning (DL). Read More

View Article and Full-Text PDF

Distinct phosphorylation profiles of tau in brains of patients with different tauopathies.

Neurobiol Aging 2021 Aug 21;108:72-79. Epub 2021 Aug 21.

Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan; Department of Dementia and Higher Brain Function, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, Japan. Electronic address:

Tauopathies are neurodegenerative diseases that are characterized by pathological accumulation of tau protein. Tau is hyperphosphorylated in the brain of tauopathy patients, and this phosphorylation is proposed to play a role in disease development. However, it has been unclear whether phosphorylation is different among different tauopathies. Read More

View Article and Full-Text PDF

Primary total ankle replacement surgery is a cost-effective intervention.

Foot (Edinb) 2021 Sep 15;49:101830. Epub 2021 Sep 15.

Department of Orthopaedics - Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, Scotland, United Kingdom.

Aims: The primary aim was to assess the cost-effectiveness of primary total ankle replacements (PTAR) in the UK. Secondary aim was to identify predictors associated with increased cost-effectiveness of PTAR.

Methods: Pre-operative and six-month post-operative data was obtained over a 90-month period across the two centres receiving adult referrals in the UK. Read More

View Article and Full-Text PDF
September 2021

High diversity of natural Dalmatian pyrethrum based on pyrethrin composition at intra- and interpopulation level.

Phytochemistry 2021 Sep 15;192:112934. Epub 2021 Sep 15.

University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska cesta 25, HR-10000 Zagreb, Croatia; Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Svetošimunska cesta 25, HR-10000 Zagreb, Croatia. Electronic address:

Seeds collected from 10 Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip. Read More

View Article and Full-Text PDF
September 2021

The CopeNYP program: A model for brief treatment of psychological distress among healthcare workers and hospital staff.

Gen Hosp Psychiatry 2021 Sep 6;73:24-29. Epub 2021 Sep 6.

Weill Cornell Medicine, Department of Psychiatry, New York Presbyterian Hospital, USA.

In the midst of the Spring 2020 initial surge of the COVID-19 pandemic in New York, members of the Psychiatry Department of Weill Cornell Medicine/NewYork-Presbyterian Hospital rapidly created and implemented a brief, behavioral skills-based intervention program, "CopeNYP", to address the immediate mental health needs of the employees of the hospital and medical school. We describe the development, implementation and evolution of this telehealth-delivered program staffed primarily by in-house clinical psychologists, postdoctoral fellows, pre-doctoral interns and counselors who were redeployed or volunteered their time to provide urgent support for employees. We discuss the challenges and lessons learned in providing brief, skills-based psychological interventions for employees subjected to chronic stress. Read More

View Article and Full-Text PDF
September 2021

Inflammatory biomarkers in very preterm infants during early intravenous paracetamol administration.

Early Hum Dev 2021 Sep 7;161:105464. Epub 2021 Sep 7.

PEDEGO Research Unit and MRC Oulu, University of Oulu, Oulu, Finland; Department of Children and Adolescents, Oulu University Hospital, Oulu, Finland.

Background: Paracetamol promotes early closure of patent ductus arteriosus (PDA), and it may affect inflammation after preterm birth.

Objective: The aim of this study was to evaluate the association between paracetamol treatment and serum inflammatory biomarkers in very preterm infants with respiratory distress.

Study Design: The infants were randomly assigned to intravenous paracetamol or placebo during the first 4 days of life, and others received a lower dose of paracetamol unblinded. Read More

View Article and Full-Text PDF
September 2021

Integrative effects based on behavior, physiology and gene expression of tritiated water on zebrafish.

Ecotoxicol Environ Saf 2021 Sep 15;225:112770. Epub 2021 Sep 15.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, PR China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, PR China. Electronic address:

Tritium is a water-soluble hydrogen isotope that releases beta rays during decay. In nature, tritium primarily exists as tritiated water (HTO), and its main source is nuclear power/processing plants. In recent decades, with the development of nuclear power industry, it is necessary to evaluate the impact of tritium on organisms. Read More

View Article and Full-Text PDF
September 2021

Considering the type and timing of breast reconstruction after mastectomy: Qualitative insights into women's decision-making.

Eur J Oncol Nurs 2021 Aug 28;54:102024. Epub 2021 Aug 28.

The University of Sydney, Faculty of Science, School of Psychology, NSW, Australia; Centre for Medical Psychology & Evidence-based Decision-making (CeMPED), School of Psychology, The University of Sydney, NSW, Australia; Psycho-oncology Co-operative Research Group (PoCoG), School of Psychology, Faculty of Science, The University of Sydney, NSW, Australia. Electronic address:

Purpose: The information women receive about the type and timing of breast reconstruction (BR) from healthcare providers is crucial to help them make an informed decision, and this is particularly important in complex cases and/or high-risk cases. This study sought to provide qualitative insights into Australian women's BR decision-making experiences.

Method: Twenty-nine women who had received a mastectomy and made decisions about BR, including the type (expander, implant and/or autologous) and timing (immediate, delayed or immediate-delayed), participated in semi-structured telephone interviews. Read More

View Article and Full-Text PDF

In vitro digestion of eight types of wholegrains and their dietary recommendations for different populations.

Food Chem 2021 Sep 7;370:131069. Epub 2021 Sep 7.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang 330047, China. Electronic address:

Wholegrains have been promoted for human consumption due to their various health benefits. However, different wholegrains vary in nutritional composition and their beneficial impact on health. In this study, we compared the in vitro starch and protein digestibility, as well as dietary fiber content of eight different wholegrains including barley, buckwheat, coix seed, foxtail millet, oat, proso millet, quinoa, and sorghum and their porridges. Read More

View Article and Full-Text PDF
September 2021

Neuromyelitis optica spectrum disorder (NMOSD) associated with cancer: A systematic review.

Mult Scler Relat Disord 2021 Aug 27;56:103227. Epub 2021 Aug 27.

Multiple Sclerosis Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

NMOSD: is a disease shown to be highly associated with other diseases such as autoimmune diseases. There are a few reports of this association with cancer. So, this systematic review aimed to obtain a broad understanding on the cancers associated in NMOSD, including the source of common perceptions and assumptions in this regard. Read More

View Article and Full-Text PDF

Borrelia duttonii-like spirochetes parasitize Meriones persicus in East Azerbaijan Province of Iran.

Ticks Tick Borne Dis 2021 Sep 4;12(6):101825. Epub 2021 Sep 4.

Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran; National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran.

In Iran, Borrelia persica and Borrelia microti/microti-like borreliae have been established as causative agents of tickborne relapsing fever (TBRF). However, the epidemiology of two previously described species, Borrelia balthazardi and Borrelia latyschewii (latychevi), has remained elusive for many years. We investigated Borrelia infection in various rodents and small mammals in the TBRF endemic East Azerbaijan Province, northwestern Iran, where B. Read More

View Article and Full-Text PDF
September 2021

Effect of ultrasound-guided selective sensory nerve blockade of the knee on pain management compared with periarticular injection for patients undergoing total knee arthroplasty: A prospective randomized controlled trial.

Knee 2021 Sep 15;33:1-10. Epub 2021 Sep 15.

Department of Anesthesiology, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society and Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Background: Ultrasound-guided selective sensory nerve blockade (SSNB) of the knee, including an adductor canal block (ACB), anterior femoral cutaneous nerve block, and infiltration between the popliteal artery and posterior capsule of the knee may provide effective motor-sparing knee analgesia for total knee arthroplasty (TKA). We hypothesized that the SSNB would manage pain better on ambulation 24 hours postoperatively compared to periarticular infiltration (PAI), when combined with postoperative continuous ACB.

Methods: Seventy-two patients undergoing elective TKA under spinal anesthesia were randomly assigned to either SSNB (SSNB group) or intraoperative PAI (PAI group). Read More

View Article and Full-Text PDF
September 2021

Reactive oxygen species (ROS) in cancer pathogenesis and therapy: An update on the role of ROS in anticancer action of benzophenanthridine alkaloids.

Biomed Pharmacother 2021 Sep 15;143:112142. Epub 2021 Sep 15.

Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, Qatar; Dermatology Institute, Academic Health System, Hamad Medical Corporation, Doha 3050, Qatar; Department of Dermatology and Venereology, Rumailah Hospital, Hamad Medical Corporation, Doha 3050, Qatar; Laboratory Animal Research Center, Qatar University, Doha 2713, Qatar. Electronic address:

Reactive oxygen species play crucial role in biological homeostasis and pathogenesis of human diseases including cancer. In this line, now it has become evident that ROS level/concentration is a major factor in the growth, progression and stemness of cancer cells. Moreover, cancer cells maintain a delicate balance between ROS and antioxidants to promote pathogenesis and clinical challenges via targeting a battery of signaling pathways converging to cancer hallmarks. Read More

View Article and Full-Text PDF
September 2021

MAPK /ERK signaling pathway: A potential target for the treatment of intervertebral disc degeneration.

Biomed Pharmacother 2021 Sep 15;143:112170. Epub 2021 Sep 15.

Second Provincial People's Hospital of Gansu, 1 Hezheng West Street, Lanzhou 730000, PR China; Affiliated Hospital of Northwest Minzu Univsity, Lanzhou 730000, PR China. Electronic address:

Intervertebral disc degeneration (IDD) is a chronic skeletal muscle degenerative disease, which is considered the main cause of low back pain. It seriously affects the quality of life of patients and consequently brings a heavy economic burden to their families and the society. Although IDD is considered a natural process in degenerative lesions, it is mainly caused by aging, trauma, genetic susceptibility and other factors. Read More

View Article and Full-Text PDF
September 2021

Nephroprotective effect of naringin in methotrexate induced renal toxicity in male rats.

Biomed Pharmacother 2021 Sep 15;143:112180. Epub 2021 Sep 15.

Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon. Electronic address:

The current work aims to study the nephroprotective potential of naringin (NG), a flavanone derived from citrus fruits, in methotrexate (MTX)-induced renal toxicity. Thirty male rats were divided into five groups; control group (IP saline), MTX group (IP single dose, 20 mg/kg), and three groups co-treated with MTX and naringin (IP daily dose; 20, 40, and 80 mg/kg, respectively). Kidney tissues were used to investigate renal function, oxidative stress, lipid peroxidation, and caspase-3 activity. Read More

View Article and Full-Text PDF
September 2021

Polycystic ovary syndrome and cardiovascular risk. Could trimethylamine N-oxide (TMAO) be a major player? A potential upgrade forward in the DOGMA theory.

Biomed Pharmacother 2021 Sep 15;143:112171. Epub 2021 Sep 15.

NGN Healthcare - New Generation Nutraceuticals s.r.l., Torrette Via Nazionale 207, 83013 Mercogliano, Avellino, Italy. Electronic address:

Several studies reported an increase in cardiovascular risk (CVR) in women with polycystic ovary syndrome (PCOS), considered primarily as the result of the combination of all the clinical features that characterize the syndrome, including hyperandrogenism, insulin resistance, diabetes, obesity chronic low-grade inflammation. Interestingly, in 2012 it has been proposed the so-called DOGMA theory, suggesting the pivotal role played by microbiota alteration in the development of PCOS. Subsequently, several authors evidenced the existence in PCOS women of a marked dysbiosis, which is related to the development of metabolic diseases and cardiovascular complications, mainly due to the production of bacteria-derived metabolites that interfere with various pathways. Read More

View Article and Full-Text PDF
September 2021

Detecting attomolar concentrations of microRNA related to myelodysplastic syndromes in blood plasma using a novel sandwich assay with nanoparticle release.

Biosens Bioelectron 2021 Sep 3;194:113613. Epub 2021 Sep 3.

Institute of Photonics and Electronics of the Czech Academy of Sciences, Chaberská 1014/57, 182 51 Prague, Czech Republic. Electronic address:

Microribonucleic acids (miRNAs) are short noncoding ribonucleic acids that have been linked with a multitude of human diseases including lung, breast, and hematological cancers. In this work, we present a novel, extremely sensitive assay for the label-free optical biosensor-based detection of miRNAs, which is based on the oligonucleotide-triggered release of nanoparticles from a sensor surface. We combine this assay (herein referred to as the nanoparticle-release (NPR) assay) with a surface plasmon resonance biosensor and show that the assay is able to enhance the specific sensor response associated with the binding of target miRNA while suppressing the interfering effects caused by the non-specific binding. Read More

View Article and Full-Text PDF
September 2021