482,843 results match your criteria in-vitro in-vivo


Prognostic value and non-neuroendocrine role of INSM1 in small cell lung cancer.

Pathol Res Pract 2021 Nov 22;229:153693. Epub 2021 Nov 22.

Institute of Pathology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China; Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:

Background: Small cell lung cancer (SCLC) is a malignant lung neuroendocrine tumor with early metastasis, rapid progression, and poor outcomes. Insulinoma-associated protein 1 (INSM1) has been an excellent marker for neuroendocrine (NE) differentiation and widely used in the diagnosis of NE neoplasms, including SCLC. However, its role beyond NE diagnostic marker remained little reported. Read More

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November 2021

Overcoming sunitinib resistance with tocilizumab in renal cell carcinoma: Discordance between in vitro and in vivo effects.

Biochem Biophys Res Commun 2021 Nov 20;586:42-48. Epub 2021 Nov 20.

Department of Urology, Princess Alexandra Hospital, Brisbane, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, Australia. Electronic address:

Sunitinib is one of the first-line multi-tyrosine kinase inhibitors for metastatic renal cell carcinoma, and resistance to sunitinib continues to be a limiting factor for the successful treatment. As interleukin-6 (IL-6) is overexpressed in sunitinib-resistant cells, the purpose of this study was to explore the potential of IL-6 inhibition with tocilizumab, an IL-6 receptor inhibitor, to overcome resistance. In vitro, two sunitinib-resistant renal cell carcinoma cell lines (Caki-1 and SN12K1) were treated with tocilizumab. Read More

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November 2021

TRAM2 promotes the malignant progression of glioma through PI3K/AKT/mTOR pathway.

Biochem Biophys Res Commun 2021 Nov 19;586:34-41. Epub 2021 Nov 19.

Department of Neurosurgery, Affiliated Hospital of Jiujiang University, Jiujiang, Jiangxi, 332000, China; Jiujiang Clinical Precision Medicine Research Center, Jiujiang, Jiangxi, 332000, China.

Molecular biomarkers play an important guidance role in the diagnosis and treatment of glioma. It has been found that TRAM2 (translocation associated membrane protein 2) drives human cancers development. Here we report that TRAM2 activity is required for malignancy properties of glioma. Read More

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November 2021

Pectins from various sources inhibit galectin-3-related cardiac fibrosis.

Curr Res Transl Med 2021 Nov 23;70(1):103321. Epub 2021 Nov 23.

Department of Cardiology, University of Groningen, University Medical Center Groningen, 9700 RB, Groningen, the Netherlands. Electronic address:

Purpose Of The Study: A major challenge in cardiology remains in finding a therapy for cardiac fibrosis. Inhibition of galectin-3 with pectins attenuates fibrosis in animal models of heart failure. The purpose of this study is to identify pectins with the strongest galectin-3 inhibitory capacity. Read More

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November 2021

Kinetically guided, ratiometric tuning of fatty acid biosynthesis.

Metab Eng 2021 Nov 23. Epub 2021 Nov 23.

Department of Chemical and Biological Engineering, University of Colorado, Boulder, 3415 Colorado Avenue, Boulder, CO, 80303, USA. Electronic address:

Cellular metabolism is a nonlinear reaction network in which dynamic shifts in enzyme concentration help regulate the flux of carbon to different products. Despite the apparent simplicity of these biochemical adjustments, their influence on metabolite biosynthesis tends to be context-dependent, difficult to predict, and challenging to exploit in metabolic engineering. This study combines a detailed kinetic model with a systematic set of in vitro and in vivo analyses to explore the use of enzyme concentration as a control parameter in fatty acid synthesis, an essential metabolic process with important applications in oleochemical production. Read More

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November 2021

HuaChanSu suppresses tumor growth and interferes with glucose metabolism in hepatocellular carcinoma cells by restraining Hexokinase-2.

Int J Biochem Cell Biol 2021 Nov 23:106123. Epub 2021 Nov 23.

School of Pharmacy, Binzhou Medical University, Yantai 264003, China. Electronic address:

Hepatocellular carcinoma (HCC) has become the sixth highly diagnosed cancer and the fourth main reason of cancer deaths worldwide. HuaChanSu, an extract from dried toad skin, exhibits good anticancer effects and has been widely used in the treatment of liver cancer. The reprogramming of glucose metabolism is one remarkable feature of hepatocellular carcinoma, and the effects of HuaChanSu on the abnormal glucose metabolism of cancer cells have not been elucidated. Read More

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November 2021

Near-infrared Triggered Ropivacaine Liposomal Gel for Adjustable and Prolonged Local Anaesthesia.

Int J Pharm 2021 Nov 23:121315. Epub 2021 Nov 23.

Department of Anesthesiology, The Second Hospital of Jilin University, Changchun, Jilin, 130012, China. Electronic address:

Local analgesics effectively allow patients to relieve postoperative pain and reduce the need for inhaled general anesthetics or opioids. Compared with other similar long-acting local anesthetics, ropivacaine (Rop) is widely used due to its potential to minimize cardiotoxicity. However, the relatively short duration of Rop efficacy, which lasts for several hours after injection, is considered insufficient for long-term acute and chronic pain treatment. Read More

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November 2021

KIAA1522 is a new biomarker of promoting the tumorigenesis and distant metastasis of colorectal carcinoma.

Cell Signal 2021 Nov 23:110202. Epub 2021 Nov 23.

Department of General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, 107 Wenhua Road, Jinan, Shandong 250012, China. Electronic address:

Purpose: Our research was absorbed into exploring the expression, clinicopathological value, biological significance and signaling pathway of KIAA1522 in colorectal carcinoma and its distant metastasis.

Materials And Methods: The expression of KIAA1522 and survival analysis in colorectal carcinoma (CRC) were assessed using GEPIA databases. Then we evaluated the expression of KIAA1522 immunohistochemically in tissue samples of 57 patients with colorectal carcinoma liver metastasis (CRLM). Read More

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November 2021

Norfloxacin suppresses Leptospira-induced inflammation through inhibiting p65 and ERK phosphorylation and NLRP3 inflammasome activation.

Microb Pathog 2021 Nov 23:105315. Epub 2021 Nov 23.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, 130062, People's Republic of China. Electronic address:

Leptospirosis is a worldwide re-emerging zoonosis caused by pathogenic Leptospira. Inflammatory storms induced by Leptospira are the reason to induce immunoparalysis and organ failures. Antibiotics are still the current mainstream treatment for leptospirosis. Read More

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November 2021

Iron overload inhibits cell proliferation and promotes autophagy via PARP1/SIRT1 signaling in endometriosis and adenomyosis.

Toxicology 2021 Nov 23:153050. Epub 2021 Nov 23.

Department of Laboratory Medicine, Huangyan Hospital of Wenzhou Medical University, Taizhou First People's Hospital, Taizhou, Zhejiang Province, China. Electronic address:

Emerging evidence suggests that excess iron accumulates in endometriotic and adenomyotic lesions. However, the role iron overload plays in the pathogenesis of endometriosis or adenomyosis remains unknown. Primary human eutopic endometrial stromal cells (EuESCs) from endometriosis or adenomyosis patients were used as the in vitro model of endometriosis or adenomyosis in this study. Read More

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November 2021

Total flavonoids of Oxytropis falcata Bunge have a positive effect on idiopathic pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway.

J Ethnopharmacol 2021 Nov 23:114858. Epub 2021 Nov 23.

Basic Medical College, Gansu University of Chinese Medicine, Gansu, Lanzhou, 730000, China; Basic Subjects of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Gansu, Lanzhou, 730000, China; Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu, Lanzhou, 730000, China. Electronic address:

Ethnopharmacological Relevance: Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease with unknown etiology. Oxytropis falcata Bunge (O. falcata) is a 1-35 cm high perennial clustered herb, also known as edaxia, has viscosity and a special smell, and is mainly distributed in the western areas of China. Read More

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November 2021

Preclinical characterization of AB-506, an inhibitor of HBV replication targeting the viral core protein.

Antiviral Res 2021 Nov 23:105211. Epub 2021 Nov 23.

Arbutus Biopharma Inc., 701 Veterans Circle, Warminster, PA, 18974, USA.

AB-506, a small-molecule inhibitor targeting the HBV core protein, inhibits viral replication in vitro (HepAD38 cells: EC of 0.077 μM, CC > 25 μM) and in vivo (HBV mouse model: ∼3.0 log reductions in serum HBV DNA compared to the vehicle control). Read More

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November 2021

Blockade of kappa-opioid receptors amplifies microglia-mediated inflammatory responses.

Pharmacol Biochem Behav 2021 Nov 23:173301. Epub 2021 Nov 23.

Department of Psychiatry, Harvard Medical School, McLean Hospital, Belmont, MA 02478, USA. Electronic address:

Brain kappa-opioid receptors (KORs) are implicated in the pathophysiology of depressive and anxiety disorders, stimulating interest in the therapeutic potential of KOR antagonists. Research on KOR function has tended to focus on KOR-expressing neurons and pathways such as the mesocorticolimbic dopamine system. However, KORs are also expressed on non-neuronal cells including microglia, the resident immune cells in the brain. Read More

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November 2021

Evidence of immunogenic cancer cell death induced by honey-processed Astragalus polysaccharides in vitro and in vivo.

Exp Cell Res 2021 Nov 23:112948. Epub 2021 Nov 23.

Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, PR China; .GDPU-HKU Zhongshan Biomedical Innovation Plaform, Zhongshan, 528437, Guangdong Province, PR China; Guangdong Engineering & Technology Research Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangzhou, 510006, Guangdong Province, PR China; Guangdong Cosmetics Engineering & Technology Research Center, Guangzhou, 510006, Guangdong Province, PR China. Electronic address:

Honey-processed Astragalus is a dosage form of Radix Astragalus mixed with honey by a traditional Chinese medicine processing method which improves immune activity. This pharmacological activity of honey-processed Astragalus polysaccharide (HP-APS) might be due to structural changes during the honey roasting process. Previously, we have prepared and characterized HP-APS and preliminarily found its anti-inflammatory effects. Read More

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November 2021

Hypoxia inducible factor-3α promotes osteosarcoma progression by activating KDM3A-mediated demethylation of SOX9.

Chem Biol Interact 2021 Nov 23:109759. Epub 2021 Nov 23.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, 450052, China.

Hypoxia/oxygen-sensing signally is closely associated with many tumor progressions, including osteosarcoma (OS). Previous research principally focused on the function of hypoxia-inducible factor (HIF)-1α and HIF-2α as the major hypoxia-associated transcription factors in OS, however, the role of HIF-3α has not been investigated. Our study found that HIF-3α was upregulated in OS tissues and cell lines. Read More

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November 2021

p-Phenylenediamine induces immediate contact allergy and non-histaminergic itch via MRGPRX2.

Chem Biol Interact 2021 Nov 23:109751. Epub 2021 Nov 23.

School of Pharmacy, Xi'an Jiaotong University, Xi'an, China; Pazhou Lab., Guangzhou, China. Electronic address:

p-phenylenediamine (PPD) is a common component of hair dye known to induce immediate allergy, even acute dermatitis and contact dermatitis. MAS-related G protein coupled receptor-X2 (MRGPRX2) in mast cells (MCs) mediates small molecular substances-induced pseudo-allergic reactions. However, the role of MRGPRX2 in PPD-induced immediate contact allergy needs further exploration. Read More

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November 2021

Exosomes from dental pulp cells attenuate bone loss in mouse experimental periodontitis.

J Periodontal Res 2021 Nov 26. Epub 2021 Nov 26.

Department of Stem Cell and Regenerative Medicine, Gifu University Graduate School of Medicine, Gifu, Japan.

Background And Objective: Exosomes are small vesicles secreted from many cell types. Their biological effects largely depend on their cellular origin and the physiological state of the originating cells. Exosomes secreted by mesenchymal stem cells exert therapeutic effects against multiple diseases and may serve as potential alternatives to stem cell therapies. Read More

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November 2021

Small intestinal sampling capsule for inflammatory bowel disease type detection and management.

Lab Chip 2021 Nov 26. Epub 2021 Nov 26.

School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Although serum and fecal biomarkers (, lactoferrin, and calprotectin) have been used in management and distinction between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS), none are proven to be a differential diagnostic tool between Crohn's disease (CD) and ulcerative colitis (UC). The main challenge with laboratory-based biomarkers in the stool test is the inability to indicate the location of the disease/inflammation in the gastrointestinal (GI) tract due to the homogenous nature of the collected fecal sample. For the first time, we have designed and developed a battery-free smart capsule that will allow targeted sampling of inflammatory biomarkers inside the gut lumen of the small intestine. Read More

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November 2021

Revisiting techniques to evaluate drug permeation through skin.

Expert Opin Drug Deliv 2021 Nov 26. Epub 2021 Nov 26.

Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science, Pilani, India, 333031.

Introduction: Investigating the transportation of a drug molecule through various layers of skin and determining the amount of drug retention in skin layers is of prime importance in transdermal and topical drug delivery. The information regarding drug permeation and retention in skin layers aids in optimizing a formulation and provides insight into the therapeutic efficacy of a formulation.

Areas Covered: This perspective covers various methods that have been explored to estimate drug/therapeutics in skin layers using , and conditions. Read More

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November 2021

Mechanism of sensitivity to TPF chemoagents and its potential alternative of erbB2 in oral cancer with GDF15 overexpression.

Cancer Sci 2021 Nov 26. Epub 2021 Nov 26.

Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.

The aim of this study was to: 1) explore the potential mechanism of cancer cell sensitivity to cisplatin, docetaxel, and 5-fluorouracil (TPF) in oral squamous cell carcinoma (OSCC) patients overexpressing growth differentiation factor 15 (GDF15); and 2) identify potential alternative agents for patients who might not benefit from inductive TPF chemotherapy. The results indicated that OSCC cells overexpressing GDF15 were sensitive to docetaxel, cisplatin, and 5-fluorouracil through a caspase-9-dependent pathway both in vitro and in vivo. Immunoprecipitation combined with mass spectrometry revealed that the erbB2 protein was a potential GDF15-binding protein, which was verified by coimmunoprecipitation. Read More

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November 2021

A composite polyphenol-rich extract improved growth performance, ruminal fermentation and immunity, while decreasing methanogenesis and excretion of nitrogen and phosphorus in growing buffaloes.

Environ Sci Pollut Res Int 2021 Nov 26. Epub 2021 Nov 26.

Department of Animal Nutrition, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana, 141004, Punjab, India.

The effects of a composite polyphenolic-rich extract (CPRE) on ruminal fermentation, nutrient utilisation, growth performance, excretion of nitrogen and phosphorus and methane emission were studied in growing buffaloes. Four herbal dry extracts prepared from Acacia arabica (babul; bark), Acacia catechu (cutch; bark), Punica granatum (pomegranate; peel) and Eugenia jambolana (Indian blackberry; seeds) were mixed in an equal proportion (1:1:1:1) to prepare the CPRE that contained mainly phenolic compounds (146 g/kg), flavonoids (41.7 g/kg) and saponins (40. Read More

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November 2021

miR27a, a fine-tuning molecule, interacts with growth hormone (GH) signaling and ornithine decarboxylase (ODC) via targeting STAT5.

Amino Acids 2021 Nov 26. Epub 2021 Nov 26.

Biotechnology Institute, Gebze Technical University, Istanbul, Turkey.

Autocrine growth hormone (GH) expression triggers cell proliferation, invasion-metastasis in vitro and in vivo models, but GH gene mutations inhibit postnatal growth. Natural polyamines (PA); putrescine, spermidine, spermine trigger cell growth and differentiation. The importance of miR27a has shown to exert a suppressive effect on ornithine decarboxylase (ODC) expression in dwarf mice models. Read More

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November 2021

Association of capsular polysaccharide locus 2 with prognosis of bacteraemia.

Emerg Microbes Infect 2021 Nov 26:1-28. Epub 2021 Nov 26.

Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.

causes healthcare-associated infections worldwide. Capsular polysaccharide (CPS) is shown an important virulence factor of both and . Capsule locus 2 (KL2) for CPS is the most common KL type and is associated with carbapenem resistance. Read More

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November 2021

Extracellular vesicles derived from adipose-derived stem cells accelerate diabetic wound healing by suppressing the expression of matrix metalloproteinase-9.

Curr Pharm Biotechnol 2021 Jul 19. Epub 2021 Jul 19.

Department of Plastic Surgery, The Second Affiliated Hospital of Nanchang University, NO.1 of Minde Road of Nanchang, Jiangxi, 330006. China.

Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Read More

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Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition.

Integr Cancer Ther 2021 Jan-Dec;20:15347354211060369

Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

Recent reports have shown that bromelain (BL), a pineapple extract, acts as an adjuvant therapy in cancer treatment and prevention of carcinogenesis. The present study was designed to investigate the possible mechanisms by which BL could radiosensitize tumor cells and in a mouse tumor model. BL has shown a significant reduction in the viability of the radioresistant human breast carcinoma (MCF-7) cell line using cell proliferation assay. Read More

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November 2021

Ovarian extracellular matrix-based hydrogel for human ovarian follicle survival in vivo: A pilot work.

J Biomed Mater Res B Appl Biomater 2021 Nov 26. Epub 2021 Nov 26.

Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.

To successfully assemble a bio-engineered ovary, we need to create a three-dimensional matrix able to accommodate isolated follicles and cells. The goal of this study was to develop an extracellular matrix hydrogel (oECM) derived from decellularized bovine ovaries able to support, in combination with alginate, human ovarian follicle survival and growth in vitro. Two different hydrogels (oECM1, oECM2) were produced and compared in terms of decellularization efficiency (dsDNA), ECM preservation (collagen and glycosaminoglycan levels), ultrastructure, rigidity, and cytotoxicity. Read More

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November 2021

Polyphenols: Potential anti-inflammatory agents for treatment of metabolic disorders.

Phytother Res 2021 Nov 25. Epub 2021 Nov 25.

Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Ample evidence highlights the potential benefits of polyphenols in health status especially in obesity-related metabolic disorders such as insulin resistance, type 2 diabetes, and cardiovascular diseases. Mechanistically, due to the key role of "Metainflammation" in the pathomechanism of metabolic disorders, recently much focus has been placed on the properties of polyphenols in obesity-related morbidities. This narrative review summarizes the current knowledge on the role of polyphenols, including genistein, chlorogenic acid, ellagic acid, caffeic acid, and silymarin in inflammatory responses pertinent to metabolic disorders and discusses the implications of this evidence for future directions. Read More

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November 2021

Convallatoxin inhibits IL-1β production by suppressing zinc finger protein 91-mediated pro-IL-1β ubiquitination and caspase-8 inflammasome activity.

Br J Pharmacol 2021 Nov 26. Epub 2021 Nov 26.

Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin Province, China.

Background And Purpose: ZFP91 positively regulates IL-1β production in macrophages and may be a potential therapeutic target to treat inflammatory-related diseases. Therefore, we investigated whether this process is modulated by convallatoxin, which is a cardiac glycoside isolated from the traditional Chinese medicinal plant Adonis amurensis Regel et Radde.

Experimental Approach: In vitro, the underlying mechanisms by which convallatoxin inhibits ZFP91-regulated IL-1β expression were investigated using molecular docking, western blotting, RT-PCR, ELISA, immunofluorescence, and immunoprecipitation assays. Read More

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November 2021

Atorvastatin restores imbalance of cluster of differentiation 4 (CD4) T cells in immune thrombocytopenia in vivo and in vitro.

Br J Haematol 2021 Nov 25. Epub 2021 Nov 25.

Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Immune thrombocytopenia (ITP) is an autoimmune haemorrhagic disease, in which the overactivation of T cells is crucial in the pathogenesis. Atorvastatin (AT), a lipid-lowering medicine, has shown promising immunomodulatory effects in certain inflammatory conditions. However, the immunoregulatory role of AT in ITP remains elusive. Read More

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November 2021

Delphinidin diminishes in vitro interferon-γ and interleukin-17 producing cells in patients with psoriatic disease.

Immunol Res 2021 Nov 25. Epub 2021 Nov 25.

Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.

The anthocyanidin delphinidin reduces psoriasiform lesions and inflammatory mediators in human cell culture systems. Its role in psoriatic disease has not yet been investigated. We assessed delphinidin's in vitro immunomodulatory effect on ex vivo stimulated peripheral blood mononuclear cells (PBMCs) from 50 individuals [26 with psoriasis, 10 with psoriatic arthritis (PsA) and 14 healthy controls (HCs)]. Read More

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November 2021