1,128 results match your criteria import component


Augmenter of Liver Regeneration regulates cellular iron homeostatis by modulating mitochondrial transport of ATP-binding cassette B8.

Elife 2021 Apr 9;10. Epub 2021 Apr 9.

Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, United States.

Chronic loss of Augmenter of Liver Regeneration (ALR) results in mitochondrial myopathy with cataracts, however, the mechanism for this disorder remains unclear. Here, we demonstrate that loss of ALR, a principal component of the MIA40/ALR protein import pathway, results in impaired cytosolic Fe/S cluster biogenesis in mammalian cells. Mechanistically, MIA40/ALR facilitates the mitochondrial import of ATP binding cassette (ABC)-B8, an inner mitochondrial membrane protein required for cytoplasmic Fe/S cluster maturation, through physical interaction with ABCB8. Read More

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Role of Cholesterol and Lipid Rafts in Cancer Signaling: A Promising Therapeutic Opportunity?

Front Cell Dev Biol 2021 19;9:622908. Epub 2021 Mar 19.

Center for Gender-Specific Medicine, Istituto Superiore di Sanità [Italian National Institute of Health], Rome, Italy.

Cholesterol is a lipid molecule that plays an essential role in a number of biological processes, both physiological and pathological. It is an essential structural constituent of cell membranes, and it is fundamental for biosynthesis, integrity, and functions of biological membranes, including membrane trafficking and signaling. Moreover, cholesterol is the major lipid component of lipid rafts, a sort of lipid-based structures that regulate the assembly and functioning of numerous cell signaling pathways, including those related to cancer, such as tumor cell growth, adhesion, migration, invasion, and apoptosis. Read More

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c-di-AMP, a likely master regulator of bacterial K homeostasis machinery, activates a K exporter.

Proc Natl Acad Sci U S A 2021 Apr;118(14)

Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal;

bis-(3',5')-cyclic diadenosine monophosphate (c-di-AMP) is a second messenger with roles in virulence, cell wall and biofilm formation, and surveillance of DNA integrity in many bacterial species, including pathogens. Strikingly, it has also been proposed to coordinate the activity of the components of K homeostasis machinery, inhibiting K import, and activating K export. However, there is a lack of quantitative evidence supporting the direct functional impact of c-di-AMP on K transporters. Read More

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The Vhl E3 ubiquitin ligase complex regulates melanisation via sima, cnc and the copper import protein Ctr1A.

Biochim Biophys Acta Mol Cell Res 2021 Mar 26;1868(7):119022. Epub 2021 Mar 26.

School of Biological Sciences, Monash University, Wellington Rd Clayton, 3800 VIC, Australia. Electronic address:

VHL encodes a tumour suppressor, which possesses E3 ubiquitin ligase activity in complex with EloC and Cul2. In tumour cells or in response to hypoxia, VHL activity is lost, causing accumulation of the transcription factor HIF-1alpha. In this study, we demonstrated that in Drosophila, Rpn9, a regulatory component of the 26 s proteasome, participates in the Vhl-induced proteasomal degradation of sima, the Drosophila orthologue of HIF-1alpha. Read More

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Determining afforestation areas by using social, economic and ecological scales.

Environ Monit Assess 2021 Mar 22;193(4):205. Epub 2021 Mar 22.

Çankırı Karatekin University Yapraklı Vocational School, Department of Forestry, 18200, Cankiri, Turkey.

Global anthropogenic damage is caused when humans aim to improve their welfare by social and economic activities. From this vantage, this paper seeks to determine priority locations for afforestation areas and carbon sinks by using socio-economic and ecological variables. Factor analysis is performed on degraded forest areas (DEGFRST), the ratio of non-forest areas to provincial general area (NFL), average of total monthly rainfall (ATMR), air pollution (PM10), the amount of migration (AMGR), annual average population density (AAPD), gross domestic product by industrial activity (I_GDP), socio-economic development index (SEDI) of provinces, export (EXP) and import (IMP) amount of provinces, average number of cars per one thousand people (ACNPT), and average electricity consumption per person (AECPP) variables for all provinces in Turkey (KMO = 0. Read More

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Hyaluronan/CD44 Axis Regulates S100A4 Mediated Mesenchymal Progenitor Cell Fibrogenicity in Idiopathic Pulmonary Fibrosis.

Am J Physiol Lung Cell Mol Physiol 2021 Mar 10. Epub 2021 Mar 10.

Department of Medicine, University of Minnesota Medical School, United States.

Despite modest improvement in patient outcomes from recent advances in pharmacotherapy targeting fibrogenic signaling pathways, IPF remains a major unsolved clinical problem. One reason for this is that available anti-fibrotic agents slow but do not arrest fibrotic progression. To arrest fibrotic progression, its obligatory drivers need to be identified. Read More

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Video laryngoscopy for out of hospital cardiac arrest.

Resuscitation 2021 Feb 25;162:143-148. Epub 2021 Feb 25.

Department of Emergency Medicine, The Ohio State University, Columbus, OH, United States.

Introduction: Endotracheal intubation is an import component of out-of-hospital cardiac arrest (OHCA) resuscitation. In this analysis, we evaluate the association of video laryngoscopy (VL) with first pass success and return of spontaneous circulation (ROSC) using a national OHCA cohort.

Methods: We analyzed 2018 data from ESO Inc. Read More

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February 2021

CHCHD4 (MIA40) and the mitochondrial disulfide relay system.

Biochem Soc Trans 2021 Feb;49(1):17-27

Department of Medicine, University of Cambridge, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, U.K.

Mitochondria are pivotal for normal cellular physiology, as they perform a crucial role in diverse cellular functions and processes, including respiration and the regulation of bioenergetic and biosynthetic pathways, as well as regulating cellular signalling and transcriptional networks. In this way, mitochondria are central to the cell's homeostatic machinery, and as such mitochondrial dysfunction underlies the pathology of a diverse range of diseases including mitochondrial disease and cancer. Mitochondrial import pathways and targeting mechanisms provide the means to transport into mitochondria the hundreds of nuclear-encoded mitochondrial proteins that are critical for the organelle's many functions. Read More

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February 2021

Structure, Function, and Interactions of the HIV-1 Capsid Protein.

Life (Basel) 2021 Jan 29;11(2). Epub 2021 Jan 29.

Department of Biochemistry & Molecular Biology, Drexel University College of Medicine, Rooms 10307, 10309, and 10315, 245 North 15th Street, Philadelphia, PA 19102, USA.

The capsid (CA) protein of the human immunodeficiency virus type 1 (HIV-1) is an essential structural component of a virion and facilitates many crucial life cycle steps through interactions with host cell factors. Capsid shields the reverse transcription complex from restriction factors while it enables trafficking to the nucleus by hijacking various adaptor proteins, such as FEZ1 and BICD2. In addition, the capsid facilitates the import and localization of the viral complex in the nucleus through interaction with NUP153, NUP358, TNPO3, and CPSF-6. Read More

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January 2021

Global hunger and climate change adaptation through international trade.

Nat Clim Chang 2020 Jul;10:829-835

Department of Earth and Environmental Sciences, University of Leuven (KU Leuven), Heverlee, Belgium.

International trade enables us to exploit regional differences in climate change impacts and is increasingly regarded as a potential adaptation mechanism. Here, we focus on hunger reduction through international trade under alternative trade scenarios for a wide range of climate futures. Under the current level of trade integration, climate change would lead to up to 55 million people who are undernourished in 2050. Read More

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Evolution of thyroid hormone distributor proteins in fish.

Authors:
Kiyoshi Yamauchi

Gen Comp Endocrinol 2021 May 4;305:113735. Epub 2021 Feb 4.

Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address:

In plasma, thyroid hormone (TH) is bound to several TH distributor proteins (THDPs), constituting a TH delivery/distribution network. Extensive studies of THDPs from tetrapods has proposed an evolutionary scenario concerning structural and functional changes in THDPs, especially for transthyretin (TTR). When assessing, in an evolutionary context, the roles of THDPs as a component constituting part of the vertebrate thyroid system, the data from fish THDPs are critical. Read More

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Activated PKB/GSK-3 synergizes with PKC- signaling in attenuating myocardial ischemia/reperfusion injury potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I.

Acta Pharm Sin B 2021 Jan 18;11(1):71-88. Epub 2020 Sep 18.

State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China.

Disrupted redox status primarily contributes to myocardial ischemia/reperfusion injury (MIRI). NRF2, the endogenous antioxidant regulator, might provide therapeutic benefits. Dihydrotanshinone-I (DT) is an active component in with NRF2 induction potency. Read More

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January 2021

The Hippo pathway component Wwc2 is a key regulator of embryonic development and angiogenesis in mice.

Cell Death Dis 2021 Jan 22;12(1):117. Epub 2021 Jan 22.

Department of Nephrology, Hypertension and Rheumatology, University Hospital Münster, Münster, Germany.

The WW-and-C2-domain-containing (WWC) protein family is involved in the regulation of cell differentiation, cell proliferation, and organ growth control. As upstream components of the Hippo signaling pathway, WWC proteins activate the Large tumor suppressor (LATS) kinase that in turn phosphorylates Yes-associated protein (YAP) and its paralog Transcriptional coactivator-with-PDZ-binding motif (TAZ) preventing their nuclear import and transcriptional activity. Inhibition of WWC expression leads to downregulation of the Hippo pathway, increased expression of YAP/TAZ target genes and enhanced organ growth. Read More

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January 2021

HIV Capsid and Integration Targeting.

Authors:
Alan N Engelman

Viruses 2021 Jan 18;13(1). Epub 2021 Jan 18.

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.

Integration of retroviral reverse transcripts into the chromosomes of the cells that they infect is required for efficient viral gene expression and the inheritance of viral genomes to daughter cells. Before integration can occur, retroviral reverse transcription complexes (RTCs) must access the nuclear environment where the chromosomes reside. Retroviral integration is non-random, with different types of virus-host interactions impacting where in the host chromatin integration takes place. Read More

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January 2021

Antivirals that target the host IMPα/β1-virus interface.

Biochem Soc Trans 2021 02;49(1):281-295

Nuclear Signaling Lab., Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Melbourne, Australia.

Although transport into the nucleus mediated by the importin (IMP) α/β1-heterodimer is central to viral infection, small molecule inhibitors of IMPα/β1-dependent nuclear import have only been described and shown to have antiviral activity in the last decade. Their robust antiviral activity is due to the strong reliance of many different viruses, including RNA viruses such as human immunodeficiency virus-1 (HIV-1), dengue (DENV), and Zika (ZIKV), on the IMPα/β1-virus interface. High-throughput compound screens have identified many agents that specifically target this interface. Read More

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February 2021

The nuclear lamina is a hub for the nuclear function of Jacob.

Mol Brain 2021 01 12;14(1). Epub 2021 Jan 12.

RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany.

Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold electron microscopy and proximity ligation assay combined with STED imaging revealed preferential association of Jacob with the inner nuclear membrane where it directly binds to LaminB1, an intermediate filament and core component of the inner nuclear membrane (INM). Read More

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January 2021

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit.

J Biol Chem 2020 Dec 3:100117. Epub 2020 Dec 3.

Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS; Ophthalmology, University of Kansas Medical Center, Kansas City, KS; Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth College, Hanover, NH. Electronic address:

Kinesin is part of the microtubule (MT)-binding motor protein superfamily, which serves important roles in cell division and intraorganellar transport. The heterotrimeric kinesin-2, consisting of the heterodimeric motor subunits KIF3A/3B and kinesin-associated protein KAP3, is highly conserved across species from the unicellular eukaryote Chlamydomonas to humans. It plays diverse roles in cargo transport including anterograde (base to tip) trafficking in cilia. Read More

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December 2020

C1QBP inhibits proliferation of porcine circovirus type 2 by restricting nuclear import of the capsid protein.

Arch Virol 2021 Mar 9;166(3):767-778. Epub 2021 Jan 9.

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.

Complement component 1 Q subcomponent-binding protein (C1QBP) has been shown to interact with the porcine circovirus type 2 (PCV2) Cap protein. Here, using yeast two-hybrid (Y2H) and co-immunoprecipitation assays, as well as laser confocal microscopy, the interaction between C1QBP and Cap was confirmed. Furthermore, overexpression of C1QBP in cells altered the intracellular location of Cap, which was observed using confocal microscopy and verified by detection of Cap in nuclear protein extracts in a Western blot assay. Read More

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L-arabinose induces the formation of viable non-proliferating spheroplasts in .

Appl Environ Microbiol 2020 Dec 18. Epub 2020 Dec 18.

Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France

, the agent of the deadly human disease cholera, propagates as a curved rod-shaped bacterium in warm waters. It is sensitive to cold, but persists in cold waters under the form of viable but non-dividing coccoidal shaped cells. Additionally, is able to form non-proliferating spherical cells in response to cell wall damage. Read More

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December 2020

In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters.

Elife 2020 12 22;9. Epub 2020 Dec 22.

Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands.

Energy-coupling factor (ECF) transporters mediate import of micronutrients in prokaryotes. They consist of an integral membrane S-component (that binds substrate) and ECF module (that powers transport by ATP hydrolysis). It has been proposed that different S-components compete for docking onto the same ECF module, but a minimal liposome-reconstituted system, required to substantiate this idea, is lacking. Read More

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December 2020

Moral reasoning perspectives of community pharmacists in situations of drug shortages.

Res Social Adm Pharm 2020 Nov 21. Epub 2020 Nov 21.

Division of Pharmacoepidemiology and Clinical Pharmacology, Department of Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB, Utrecht, the Netherlands. Electronic address:

Background: Drug shortages affect health systems worldwide. Research in community pharmacy has focused on the nature, extent and impact of these shortages on patients and pharmacists. However, pharmacists' moral reasoning in situations of drug shortages has not been addressed. Read More

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November 2020

Phosphatidylinositol synthesis, its selective salvage, and inter-regulation of anionic phospholipids in Toxoplasma gondii.

Commun Biol 2020 Dec 10;3(1):750. Epub 2020 Dec 10.

Department of Molecular Parasitology, Faculty of Life Sciences, Humboldt University, Berlin, Germany.

Phosphatidylinositol (PtdIns) serves as an integral component of eukaryotic membranes; however, its biosynthesis in apicomplexan parasites remains poorly understood. Here we show that Toxoplasma gondii-a common intracellular pathogen of humans and animals-can import and co-utilize myo-inositol with the endogenous CDP-diacylglycerol to synthesize PtdIns. Equally, the parasite harbors a functional PtdIns synthase (PIS) containing a catalytically-vital CDP-diacylglycerol phosphotransferase motif in the Golgi apparatus. Read More

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December 2020

Tim17 Updates: A Comprehensive Review of an Ancient Mitochondrial Protein Translocator.

Biomolecules 2020 12 7;10(12). Epub 2020 Dec 7.

Meharry Medical College, Department of Microbiology, Immunology, and Physiology, 1005 Dr. D.B. Todd, Jr., Blvd, Nashville, TN 37208, USA.

The translocases of the mitochondrial outer and inner membranes, the TOM and TIMs, import hundreds of nucleus-encoded proteins into mitochondria. TOM and TIMs are multi-subunit protein complexes that work in cooperation with other complexes to import proteins in different sub-mitochondrial destinations. The overall architecture of these protein complexes is conserved among yeast/fungi, animals, and plants. Read More

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December 2020

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit.

J Biol Chem 2020 Nov 24. Epub 2020 Nov 24.

Geisel School of Medicine at Dartmouth College, United States.

Kinesin is part of the microtubule (MT)-binding motor protein superfamily, which serves important roles in cell division and intraorganellar transport. The heterotrimeric kinesin-2, consisting of the heterodimeric motor subunits KIF3A/3B and kinesin-associated protein KAP3, is highly conserved across species from the unicellular eukaryote to humans. It plays diverse roles in cargo transport including anterograde (base to tip) trafficking in cilia. Read More

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November 2020

MFSD12 mediates the import of cysteine into melanosomes and lysosomes.

Nature 2020 12 18;588(7839):699-704. Epub 2020 Nov 18.

Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

Dozens of genes contribute to the wide variation in human pigmentation. Many of these genes encode proteins that localize to the melanosome-the organelle, related to the lysosome, that synthesizes pigment-but have unclear functions. Here we describe MelanoIP, a method for rapidly isolating melanosomes and profiling their labile metabolite contents. Read More

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December 2020

Expansion of the evolutionarily conserved network of J-domain proteins in the Arabidopsis mitochondrial import complex.

Plant Mol Biol 2021 Mar 18;105(4-5):385-403. Epub 2020 Nov 18.

Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, Room Number 117 AB3, IISER Bhopal, Bhopal Bypass Road, Bhopal, MP, 462066, India.

Key Message: We report that discriminate interaction between the expanded mitochondrial chaperone network and variability in their expression might determine their functional specificities and impart robustness to mitochondrial import processes in plants. Mitochondrial Hsp70 (mtHsp70), the central component of the pre-sequence associated motor (PAM) complex, is crucial for the import of proteins to the mitochondrial matrix. Activity of mtHsp70 is regulated by a heterodimeric complex of two J-domain proteins (JDPs), Pam18 and Pam16. Read More

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Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex.

J Mol Biol 2020 12 14;432(24):166713. Epub 2020 Nov 14.

Department of Medicine, University of Udine, 33100 Udine, Italy; Laboratory of Mitochondrial Biogenesis, Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland. Electronic address:

Mitochondria are essential cellular organelles that import the majority of proteins to sustain their function in cellular metabolism and homeostasis. Due to their role in oxidative phosphorylation, mitochondria are constantly affected by oxidative stress. Stability of mitochondrial DNA (mtDNA) is essential for mitochondrial physiology and cellular well-being and for this reason mtDNA lesions have to be rapidly recognized and repaired. Read More

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December 2020

Synthetic Monopartite Peptide That Enables the Nuclear Import of Genes Delivered by the Neurotensin-Polyplex Vector.

Mol Pharm 2020 12 30;17(12):4572-4588. Epub 2020 Oct 30.

Programa de Doctorado en Nanociencias y Nanotecnología, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional, No. 2508, San Pedro Zacatenco, 07360 Ciudad de México, Mexico.

Neurotensin (NTS)-polyplex is a multicomponent nonviral vector that enables gene delivery via internalization of the neurotensin type 1 receptor (NTSR1) to dopaminergic neurons and cancer cells. An approach to improving its therapeutic safety is replacing the viral karyophilic component (peptide KPSV40; MAPTKRKGSCPGAAPNKPK), which performs the nuclear import activity, by a shorter synthetic peptide (KPRa; KMAPKKRK). We explored this issue and the mechanism of plasmid DNA translocation through the expression of the green fluorescent protein or red fluorescent protein fused with KPRa and internalization assays and whole-cell patch-clamp configuration experiments in a single cell together with importin α/β pathway blockers. Read More

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December 2020

The selectivity filter of the mitochondrial protein import machinery.

BMC Biol 2020 10 29;18(1):156. Epub 2020 Oct 29.

Institute for Biochemistry and Pathobiochemistry, Ruhr-University Bochum, 44780, Bochum, Germany.

Background: The uptake of newly synthesized nuclear-encoded mitochondrial proteins from the cytosol is mediated by a complex of mitochondrial outer membrane proteins comprising a central pore-forming component and associated receptor proteins. Distinct fractions of proteins initially bind to the receptor proteins and are subsequently transferred to the pore-forming component for import. The aim of this study was the identification of the decisive elements of this machinery that determine the specific selection of the proteins that should be imported. Read More

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October 2020

CosinorPy: a python package for cosinor-based rhythmometry.

Authors:
Miha Moškon

BMC Bioinformatics 2020 Oct 29;21(1):485. Epub 2020 Oct 29.

Faculty of Computer and Information Science, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia.

Background: Even though several computational methods for rhythmicity detection and analysis of biological data have been proposed in recent years, classical trigonometric regression based on cosinor still has several advantages over these methods and is still widely used. Different software packages for cosinor-based rhythmometry exist, but lack certain functionalities and require data in different, non-unified input formats.

Results: We present CosinorPy, a Python implementation of cosinor-based methods for rhythmicity detection and analysis. Read More

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October 2020