2,574 results match your criteria implantation window


Prevalence of Macrophages Within the Cochlear Vessels Following Cochlear Implantation in the Human: An Immunohistopathological Study Using Anti-Iba1 Antibody.

Otol Neurotol 2021 Jul 28. Epub 2021 Jul 28.

Otopathology Laboratory, Department of Otolaryngology-Head and Neck Surgery, Massachusetts Eye and Ear Department of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, Massachusetts Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara, Nara, Japan.

Hypothesis: The prevalence of monocyte-derived macrophages within cochlear vessels may increase following cochlear implantation.

Background: Recently, we reported an increase in the number of ionized calcium-binding adaptor molecule 1 (Iba1)-positive macrophages in selected cochlear sites such as the osseous spiral lamina and Rosenthal's canal following cochlear implantation. Activation of the immune system induces the recruitment of monocyte-derived macrophages. Read More

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Screening of Variants in the Transcript Profile of Eutopic Endometrium from Infertile Women with Endometriosis during the Implantation Window.

Rev Bras Ginecol Obstet 2021 Jun 27;43(6):457-466. Epub 2021 Jul 27.

Department of Gynecology and Obstetrics, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

Objective:  Abnormalities in the eutopic endometrium of women with endometriosis may be related to disease-associated infertility. Although previous RNA-sequencing analysis did not show differential expression in endometrial transcripts of endometriosis patients, other molecular alterations could impact protein synthesis and endometrial receptivity. Our aim was to screen for functional mutations in the transcripts of eutopic endometria of infertile women with endometriosis and controls during the implantation window. Read More

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Cardiac Arrest Secondary to Traumatic Aortopulmonary Window During Transcatheter Pulmonary Valve Implantation in Supported Ross.

JACC Case Rep 2019 Dec 18;1(5):746-750. Epub 2019 Dec 18.

Herma Heart Institute, Children's Hospital of Wisconsin and Medical College of Wisconsin, Milwaukee, Wisconsin.

We present a patient with a supported Ross procedure and severe pulmonary homograft stenosis who developed cardiac arrest while undergoing transcatheter pulmonary valve replacement and was found to have a large iatrogenic aortopulmonary window. Cardiopulmonary resuscitation was initiated followed by covered stent placement, extracorporeal membrane oxygenation support, and ultimately emergent surgery with a good outcome. (). Read More

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December 2019

In vivo imaging of the microglial landscape after whole brain radiation therapy.

Int J Radiat Oncol Biol Phys 2021 Jul 24. Epub 2021 Jul 24.

Department of Radiation Oncology, University of Rochester, Rochester, NY. Electronic address:

Purpose: Whole brain radiation therapy (WBRT) is an important treatment for patients with multiple brain metastases, but can also cause cognitive deterioration. Microglia, the resident immune cells of the brain, promote a pro-inflammatory environment and likely contribute to cognitive decline after WBRT. To investigate the temporal dynamics of the microglial reaction in individual mice to WBRT, we developed a novel in vivo experimental model using cranial window implants and longitudinal imaging. Read More

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Morphological features of the secretory phase endometrium in women with unexplained infertility.

Ultrastruct Pathol 2021 Jul 27:1-14. Epub 2021 Jul 27.

Histology and Embryology Department, Çukurova University, Faculty of Medicine, Adana, Turkey.

In this study, we evaluated the structural features of the endometrial tissues, the immunohistochemical expression of MUC-1, which plays an important role in implantation, and the biochemical markers during the implantation window. Randomly chosen 18 fertile and 18 unexplained infertile women that have 27-32 days long menstrual cycle, normal hormonal values, normal USG findings of ovary and endometrium were included. Five, six, and seven days after ovulation, endometrial biopsies were taken and prepared in accordance with light and electron microscopy tissue preparation methods. Read More

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Round window anatomy predicts ease of cochlear implantation in children.

Int J Pediatr Otorhinolaryngol 2021 Jul 21;149:110852. Epub 2021 Jul 21.

Division of Pediatric Otolaryngology, Children's National Health System, Washington, DC, USA. Electronic address:

Objectives: We aim to evaluate the utility of the Round Window Angle (RWA) as a predictor of difficulty and operative time in cochlear implantation.

Methods: A retrospective study of pediatric patients that underwent cochlear implantation and CT temporal bone imaging from January 2008 to November 2019. Correlation, univariate, and multivariate analysis were conducted. Read More

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Endoscopy-Assisted Transmeatal Cochlear Implantation in Multiple Ear Deformities.

J Int Adv Otol 2021 Jul;17(4):376-379

Department of Otolaryngology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

To study congenitally deaf children with inner ear malformations that usually have comorbid anomalous facial nerves and middle ear deformities. To determine the feasibility of endoscopy-assisted transmeatal cochlear implantation with the purpose of reducing the risks of iatrogenic facial nerve injury. This report presents a unique technique in a pediatric case with multiple ear anomalies: microtia, cochlear hypoplasia with an aberrant facial nerve, a sigmoid sinus deformity leading to a narrow mastoid cavity, and a flat promontory wall without round window. Read More

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A Composite Lactide-Mineral 3D-Printed Scaffold for Bone Repair and Regeneration.

Front Cell Dev Biol 2021 9;9:654518. Epub 2021 Jul 9.

Department of Surgery, Division of Orthopaedic Surgery, McGill University, Montreal, QC, Canada.

Orthopedic tumor resection, trauma, or degenerative disease surgeries can result in large bone defects and often require bone grafting. However, standard autologous bone grafting has been associated with donor site morbidity and/or limited quantity. As an alternate, allografts with or without metallic or polyether-etherketone have been used as grafting substitutes. Read More

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Preparation of the Endometrium for Frozen Embryo Transfer: A Systematic Review.

Front Endocrinol (Lausanne) 2021 9;12:688237. Epub 2021 Jul 9.

Department of Obstetrics and Gynecology, Hacettepe University School of Medicine, Ankara, Turkey.

Despite the worldwide increase in frozen embryo transfer, the search for the best protocol to prime endometrium continues. Well-designed trials comparing various frozen embryo transfer protocols in terms of live birth rates, maternal, obstetric and neonatal outcome are urgently required. Currently, low-quality evidence indicates that, natural cycle, either true natural cycle or modified natural cycle, is superior to hormone replacement treatment protocol. Read More

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Trophoblast derived extracellular vesicles specifically alter the transcriptome of endometrial cells and may constitute a critical component of embryo-maternal communication.

Reprod Biol Endocrinol 2021 Jul 21;19(1):115. Epub 2021 Jul 21.

Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, Tartu University, Tartu, Estonia.

Background: The period of time when the embryo and the endometrium undergo significant morphological alterations to facilitate a successful implantation-known as "window of implantation"-is a critical moment in human reproduction. Embryo and the endometrium communicate extensively during this period, and lipid bilayer bound nanoscale extracellular vesicles (EVs) are purported to be integral to this communication.

Methods: To investigate the nature of the EV-mediated embryo-maternal communication, we have supplemented trophoblast analogue spheroid (JAr) derived EVs to an endometrial analogue (RL 95-2) cell layer and characterized the transcriptomic alterations using RNA sequencing. Read More

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A Permanent Window for Investigating Cancer Metastasis to the Lung.

J Vis Exp 2021 Jul 1(173). Epub 2021 Jul 1.

Department of Anatomy and Structural Biology, Einstein College of Medicine / Montefiore Medical Center; Gruss-Lipper Biophotonics Center, Einstein College of Medicine / Montefiore Medical Center; Integrated Imaging Program, Einstein College of Medicine / Montefiore Medical Cente;

Metastasis, accounting for ~90% of cancer-related mortality, involves the systemic spread of cancer cells from primary tumors to secondary sites such as the bone, brain, and lung. Although extensively studied, the mechanistic details of this process remain poorly understood. While common imaging modalities, including computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI), offer varying degrees of gross visualization, each lacks the temporal and spatial resolution necessary to detect the dynamics of individual tumor cells. Read More

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Identifying Biomarkers from Transcriptomic Signatures in Renal Allograft Biopsies Using Deceased and Living Donors.

Front Immunol 2021 1;12:657860. Epub 2021 Jul 1.

Department of Cardiovascular Sciences, University of Leicester, Leicester, United Kingdom.

The survival of transplant kidneys using deceased donors (DD) is inferior to living donors (LD). In this study, we conducted a whole-transcriptome expression analysis of 24 human kidney biopsies paired at 30 minutes and 3 months post-transplantation using DD and LD. The transcriptome profile was found significantly different between two time points regardless of donor types. Read More

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Iatrogenic Third Window After Retrosigmoid Approach to a Vestibular Schwannoma Managed with Cochlear Implantation.

Otol Neurotol 2021 Jul 9. Epub 2021 Jul 9.

Department of Otolaryngology-Head & Neck Surgery, Mayo Clinic, Phoenix, Arizona Department of Otolaryngology-Head & Neck Surgery, NYU School of Medicine, New York, New York.

Objective: To present a case of an iatrogenic inner ear third window after vestibular schwannoma microsurgery.

Patients: A 42-year-old male presented 9-months after left-sided retrosigmoid approach for an intracanalicular vestibular schwannoma with hearing-preservation attempt performed elsewhere. Immediately postoperatively, he developed the following disabling and persistent symptoms on the ipsilateral side: autophony, pulsatile tinnitus, high-pitched ringing tinnitus, and hearing his footsteps. Read More

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The Low Window Technique: Comparison between Manual and CAD/CAM-guided Design.

J Contemp Dent Pract 2021 Apr 1;22(4):400-405. Epub 2021 Apr 1.

Department of Cariology and Comprehensive Care, New York University College of Dentistry, New York, United States.

Aim: The aim of this bench study was to provide quantitative data addressing the difference between the manual low window design and the corresponding computer-aided design and computer-aided manufacturing (CAD/CAM)-guided design.

Materials And Methods: Five cone-beam computed tomography (CBCT) scans of as many patients (two males and three females, age range: 61-78 years) with partially edentulous maxilla to be rehabilitated through sinus augmentation, one- or two-step implant placement, and implant-supported prostheses were used to 3D-print the corresponding five maxillary stereolithographic models. Five independent highly skilled maxillofacial surgeons, who were provided with the patients' orthopantomographs, drew on the models the access windows for sinus augmentation according to the "high" (standard) and the low window design both by free-hand and using a surgical guide prepared by computer-guided design. Read More

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Inconsistencies between endometrial receptivity assay (ERA) and Adhesio-RT test for the window of implantation in women with repetitive failed donor oocyte embryo transfers.

Obstet Gynecol Sci 2021 Jul 15. Epub 2021 Jul 15.

Department of Obstetrics and Gynecology, McGill University Health Centre Reproductive Centre, Montreal, Canada.

Two commercially available second-generation endometrial receptivity assays using microarray analysis or next-generation sequencing are available in the market: endometrial receptivity assay (ERA) (Igenomix Laboratories) and Adhesio RT (OVO Laboratories). Little is known about how the results of these tests compare. We present a case of a subject with repetitive failed donor oocyte embryo transfer, who underwent evaluation of endometrial receptivity using both the Adhesio and ERA tests. Read More

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Severe male factor in fertilization: definition, prevalence, and treatment. An update.

Asian J Androl 2021 Jul 6. Epub 2021 Jul 6.

Clinica Valle Giulia, GeneraLife IVF Centers, Via G. De Notaris, Rome 2B 00197, Italy.

Infertility affects 10%-15% of couples worldwide. Of all infertility cases, 20%-70% are due to male factors. In the past, men with severe male factor (SMF) were considered sterile. Read More

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MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development.

Reprod Biol Endocrinol 2021 Jul 13;19(1):109. Epub 2021 Jul 13.

Department of Immunobioquímica, Instituto Nacional de Perinatología "Isidro Espinosa de los Reyes" (INPerIER), Ciudad de México, México.

Background: Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos have aberrant morphology. Read More

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Jagged1 regulates endometrial receptivity in both humans and mice.

FASEB J 2021 08;35(8):e21784

Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia.

The human endometrium undergoes cycle-dependent changes and is only receptive to an implanting blastocyst within a narrow window of 2-4 days in the mid-secretory phase. Such functional changes require delicate interplay between a diversity of factors including cytokines and signaling pathways. The Notch signaling pathway members are expressed in human endometrium. Read More

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Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice.

J Vis Exp 2021 Jun 22(172). Epub 2021 Jun 22.

Department of Neurological Sciences, University of Nebraska Medical Center;

To fully understand the cellular physiology of neurons and glia in behaving animals, it is necessary to visualize their morphology and record their activity in vivo in behaving mice. This paper describes a method for the implantation of a chronic cranial window to allow for the longitudinal imaging of brain cells in awake, head-restrained mice. In combination with genetic strategies and viral injections, it is possible to label specific cells and regions of interest with structural or physiological markers. Read More

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GLUT4 in Mouse Endometrial Epithelium: Roles in Embryonic Development and Implantation.

Front Physiol 2021 25;12:674924. Epub 2021 Jun 25.

Department of Physiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

GLUT4 is involved in rapid glucose uptake among various kinds of cells to contribute to glucose homeostasis. Prior data have reported that aberrant glucose metabolism by GLUT4 dysfunction in the uterus could be responsible for infertility and increased miscarriage. However, the expression and precise functions of GLUT4 in the endometrium under physiological conditions remain unknown or controversial. Read More

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A neck-preserving short stem better reconstructs the centre of rotation than straight stems: a computed tomography-based cadaver study.

Arch Orthop Trauma Surg 2021 Jul 6. Epub 2021 Jul 6.

Expert and Product Management Department, BBraun Aesculap, Tuttlingen, Germany.

Introduction: Short femoral hip stems with a metaphyseal anchoring concept have been designed to treat younger patients with good bone quality. The aim of this study was to reconstruct the centre of rotation and soft tissue balancing and preserve bone in the long-term perspective.

Materials And Methods: Eighteen human femurs were randomised into three groups: (1) metaphyseal anchoring short stem, (2) shortened straight stem, (3) straight stem). Read More

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Electrically evoked auditory brainstem responses to electrical stimulation at round window membrane in congenitally deaf children at different ages.

Int J Pediatr Otorhinolaryngol 2021 Jun 30;148:110821. Epub 2021 Jun 30.

Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China. Electronic address:

Objectives: To investigate the usefulness of measuring the electrically evoked auditory brainstem responses (eABRs) to electrical stimulation at the round window niche (RWN) and round window membrane (RWM) and the effect of deafness duration on functions of the auditory pathway to the level of the brainstem.

Methods: According to the age at cochlear implantation (CI), 99 children with profound sensorineural hearing loss were divided into four groups: ≤12 months (group A), 13-36 months (group B), 37-60 months (group C) and >60 months (group D). The eABRs were evoked by electrical stimulation at RWN and RWM during the operation of CI. Read More

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Age-Dependent Maturation Efficacy of Human Oocytes - Is There an Optimal Age?

Front Cell Dev Biol 2021 18;9:667682. Epub 2021 Jun 18.

Fertility Preservation Service, Department of Obstetrics and Gynecology, Hadassah Ein Kerem Medical Center and Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.

maturation of oocytes from antral follicles seen during tissue harvesting is a fertility preservation technique with potential advantages over ovarian tissue cryopreservation (OTC), as mature frozen and later thawed oocyte used for fertilization poses decreased risk of malignant cells re-seeding, as compared to ovarian tissue implantation. We previously demonstrated that maturation (IVM) performed following OTC in fertility preservation patients, even in pre-menarche girls, yields a fair amount of oocytes available for IVM and freezing for future use. We conducted a retrospective cohort study, evaluating IVM outcomes in chemotherapy naïve patients referred for fertility preservation by OTC that had oocyte collected from the medium with attempted IVM. Read More

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Endometrial gene expression profiling of recurrent implantation failure after in vitro fertilization.

Mol Biol Rep 2021 Jun 3;48(6):5075-5082. Epub 2021 Jul 3.

Molecular Biology and Genetics Department, Faculty of Science, Istanbul University, 34134, Istanbul, Fatih, Turkey.

Recurrent implantation failure (RIF) is diagnosed when good-quality embryos repeatedly fail to implant after transfer in several in vitro fertilization (IVF) treatment cycles. Different expression profiles in maternal mRNAs could be referring to many diseases including RIF. This study aimed to reveal significantly dysregulated selected genes expression between healthy fertile women and RIF patients in the implantation window days of the natural menstrual cycle. Read More

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Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol.

Reprod Biol Endocrinol 2021 Jul 2;19(1):99. Epub 2021 Jul 2.

Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, China.

Background: The gonadotropin-releasing hormone (GnRH) antagonist protocol for in vitro fertilization (IVF) often leads to lower pregnancy rates compared to the GnRH agonist protocol. Decreased endometrial receptivity is one reason for the lower success rate, but the mechanisms underlying this phenomenon remain poorly understood. The S100 calcium protein P (S100P) is a biomarker for endometrial receptivity. Read More

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Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.

Front Vet Sci 2021 15;8:680539. Epub 2021 Jun 15.

Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), Madrid, Spain.

Embryonic losses constitute a major burden for reproductive efficiency of farm animals. Pregnancy losses in ungulate species, which include cattle, pigs, sheep and goats, majorly occur during the second week of gestation, when the embryo experiences a series of cell differentiation, proliferation, and migration processes encompassed under the term conceptus elongation. Conceptus elongation takes place following blastocyst hatching and involves a massive proliferation of the extraembryonic membranes trophoblast and hypoblast, and the formation of flat embryonic disc derived from the epiblast, which ultimately gastrulates generating the three germ layers. Read More

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A Wirelessly Controlled Scalable 3D-Printed Microsystem for Drug Delivery.

Pharmaceuticals (Basel) 2021 Jun 4;14(6). Epub 2021 Jun 4.

Department of Microsystems Engineering, Rochester Institute of Technology, Rochester, NY 14623, USA.

Here we present a 3D-printed, wirelessly controlled microsystem for drug delivery, comprising a refillable microreservoir and a phase-change peristaltic micropump. The micropump structure was inkjet-printed on the back of a printed circuit board around a catheter microtubing. The enclosure of the microsystem was fabricated using stereolithography 3D printing, with an embedded microreservoir structure and integrated micropump. Read More

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Morphological classification of crista fenestra of round window corridor during pediatric cochlear implantation.

Int J Pediatr Otorhinolaryngol 2021 Jun 26;148:110816. Epub 2021 Jun 26.

Otolaryngology, Otolaryngology Department, Alexandria University, Egypt.

Objectives: This study aimed to document the observation of the Crista Fenestra's morphological types (CF) of the round window and to detect its impact during cochlear implant operation.

Study Design: A prospective descriptive cohort study.

Settings: We conducted this study at tertiary referral institutions in Egypt. Read More

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Early pediatric Cochlear implantation: An update.

Laryngoscope Investig Otolaryngol 2021 Jun 12;6(3):512-521. Epub 2021 May 12.

Division of Pediatric Otolaryngology Nationwide Children's Hospital Columbus Ohio USA.

The criteria and candidacy for pediatric cochlear implantation (CI) has significantly transformed over the past few decades and continues to evolve with technological advancements, and recognition of benefit in more diverse populations. Prolonged auditory deprivation among patients with profound sensorineural hearing loss has been shown to cause widespread degeneration in the central auditory system. Thus, there is increasing evidence advocating for earlier implantation within a critical neuroplastic window. Read More

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Global transcriptomic changes occur in uterine fluid-derived extracellular vesicles during the endometrial window for embryo implantation.

Hum Reprod 2021 Jul;36(8):2249-2274

Reproductive Sciences Laboratory, Obstetrics and Gynecology Unit, IRCCS San Raffaele Scientific Institute, Milan, Ital, Milan, Italy.

Study Question: Are uterine fluid-derived extracellular vesicles (UF-EVs) a 'liquid biopsy' reservoir of biomarkers for real-time monitoring of endometrial status?

Summary Answer: The transcriptomic cargo of UF-EVs reflects the RNA profile of the endometrial tissue as well as changes between the non-receptive and the receptive phase, possibly supporting its use for a novel endometrial receptivity test.

What Is Known Already: EVs have been previously isolated from uterine fluid, where they likely contribute to the embryo-endometrium crosstalk during implantation. Based on a meta-analysis of studies on endometrial tissue implantation-associated genes and the human exosomes database, 28 of the 57 transcripts considered as receptivity markers refer to proteins present in human exosomes. Read More

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