966 results match your criteria impact centrifugation

Impact of centrifugation time and pneumatic tube transport on plasma concentrations of direct oral anticoagulants.

Int J Lab Hematol 2021 Oct 12. Epub 2021 Oct 12.

Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.

Introduction: Rapid results are needed when plasma concentrations of direct oral anticoagulants (DOACs) are required in acute clinical settings. We evaluated the impact of centrifugation time and pneumatic tube transport on DOAC plasma concentrations with the overall aim of reducing turnaround time.

Methods: Blood samples were spiked with rivaroxaban, apixaban or dabigatran in a low and a high concentration prior to centrifugation for 25 minutes (3163 g) or 5 minutes (3000 g) (n = 20 for each DOAC). Read More

View Article and Full-Text PDF
October 2021

Extracellular vesicles from maternal uterine cells exposed to risk factors cause fetal inflammatory response.

Cell Commun Signal 2021 Oct 7;19(1):100. Epub 2021 Oct 7.

Division of Maternal-Fetal Medicine and Perinatal Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, 301 University Blvd., Galveston, TX, 77555-1062, USA.

Background: Fetal cell-derived exosomes (extracellular vesicles, 40-160 nm) are communication channels that can signal parturition by inducing inflammatory changes in maternal decidua and myometrium. Little is known about maternal cell-derived exosomes and their functional roles on the fetal side. This study isolated and characterized exosomes from decidual and myometrial cells grown under normal and inflammatory/oxidative stress conditions and determined their impact on fetal membrane cells. Read More

View Article and Full-Text PDF
October 2021

How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.

J Biotechnol 2021 Sep 29;341:121-128. Epub 2021 Sep 29.

Department of Materials and Bioprocesses Engineering, School of Chemical Engineering, University of Campinas, P.O. Box 6066, 13083-852, Campinas, SP, Brazil.

Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas. Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components. Read More

View Article and Full-Text PDF
September 2021

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury.

J Proteome Res 2021 Sep 23. Epub 2021 Sep 23.

SIGNATOPE GmbH, Reutlingen 72770, Germany.

Macrophage colony stimulating factor 1 receptor (MCSF1R), osteopontin (OPN), high-mobility group protein B1 (HMGB1), glutamate dehydrogenase (GLDH), keratin 18 (K18), and caspase-cleaved keratin 18 (ccK18) are considered promising mechanistic biomarkers for the diagnosis of drug-induced liver injury. Here, we aim to elucidate the impact of the sample matrix and handling on the quantification of these emerging protein biomarkers. We investigated effects such as time from collection to centrifugation during serum (± gel) or EDTA plasma preparation on two assay platforms: immunoaffinity liquid chromatography mass spectrometric assays and sandwich immunoassays. Read More

View Article and Full-Text PDF
September 2021

Application of Oleogel and Conventional Fats for Ultrasound-assisted Obtaining of Vegan Creams.

J Oleo Sci 2021 Oct 8;70(10):1495-1507. Epub 2021 Sep 8.

Department of Physicochemistry and Material Technology, Kazimierz Pulaski University of Technologies and Humanities.

The purpose of this study was to determine the impact of the fat system type (milk fat - MF, palm oil - PO or oleogel - OG, i.e. RO-LO - rapeseed oil and linseed oil mixture structured by candelilla wax) on the properties of soy creams, in comparison with dairy cream. Read More

View Article and Full-Text PDF
October 2021

Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma.

BMC Cancer 2021 Sep 8;21(1):1003. Epub 2021 Sep 8.

Department of Otorhinolaryngology Head and Neck surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.

Background: Nasopharyngeal carcinoma (NPC) is one of the most common malignancies in head and neck. Platinum-based chemotherapy is an important treatment for NPC. However, the molecular mechanism of resistance to platinum drug remains unknown. Read More

View Article and Full-Text PDF
September 2021

Downregulation of let-7 by Electrical Acupuncture Increases Protein Synthesis in Mice.

Front Physiol 2021 20;12:697139. Epub 2021 Aug 20.

Renal Division, Department of Medicine, Emory University, Atlanta, GA, United States.

Background: Our previous study found that acupuncture with low frequency electrical stimulation (Acu/LFES) prevents muscle atrophy by attenuation of protein degradation in mice. The current study examines the impact of Acu/LFES on protein synthesis.

Method: C57/BL6 mice received Acu/LFES treatment on hindlimb for 30 min once. Read More

View Article and Full-Text PDF

The effect of the anticoagulant on the cellular composition and growth factor content of platelet-rich plasma.

Cell Tissue Bank 2021 Aug 28. Epub 2021 Aug 28.

Lokman Hekim Medical Services, Alab Laboratories, Ankara, Turkey.

Background: The cellular and biochemical composition of the platelet rich plasma (PRP) may impact its regenerative capacity. PRP composition have been shown to vary substantially among different separation systems and protocols. The type and the dose of anticoagulant might affect the content of PRP. Read More

View Article and Full-Text PDF

Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression.

Stem Cell Res Ther 2021 Aug 28;12(1):481. Epub 2021 Aug 28.

Department of Medical and Surgical Sciences for Children and Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy.

Introduction: Adipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Read More

View Article and Full-Text PDF

Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.

RNA 2021 Aug 27. Epub 2021 Aug 27.

Rowan University, SOM

Stress-induced molecular damage to ribosomes can impact protein synthesis in cells, but cell-based assays do not provide a clear way to distinguish the effects of ribosome damage from stress responses and damage to other parts of the translation machinery. Here we describe a detailed protocol for the separation of yeast ribosomes from other translational machinery constituents, followed by reconstitution of the translation mixture in vitro. This technique, which we refer to as Ribosome Separation and Reconstitution (RSR), allows chemical modifications of yeast ribosomes without compromising other key translational components. Read More

View Article and Full-Text PDF

Mesenchymal Stem Cells and Platelet Rich Plasma Therapy to Treat Leak After Sleeve Gastrectomy.

J Surg Res 2021 Aug 17;268:405-410. Epub 2021 Aug 17.

Digestive Surgery and Liver Transplantation Unit, University Hospital of Nice, Nice, France.

Background: One of the most feared and life-threatening complications after sleeve gastrectomy (SG) is staple line leak, with an incidence between 1 and 4%. Stable patients may be managed conservatively, with antibiotics, percutaneous drainage and endoscopy-based treatment. We propose mesenchymal stem cells (MSC) and platelet rich plasma (PRP) therapy as an innovative technique to treat leak after SG. Read More

View Article and Full-Text PDF

Development of a Method to Detect in the Blood of Farmed Deer Using Actiphage® Rapid.

Front Vet Sci 2021 29;8:665697. Epub 2021 Jul 29.

School of Biosciences, University of Nottingham, Loughborough, United Kingdom.

subsp paratuberculosis (MAP) is the causative agent of Johne's disease, which is an economically and clinically relevant pathogen for commercial deer production. The purpose of this study was to develop a method that could be used to rapidly detect MAP infection in deer using the Actiphage Rapid blood test. This test has previously been used to detect MAP in cattle blood following the purification of buffy coat using Ficoll gradients, however this method is quite laborious and costly. Read More

View Article and Full-Text PDF

Pu-239 Accumulation in E. Coli and P. Putida Grown in Liquid Cultures.

Health Phys 2021 Nov;121(5):484-493

Center for Nuclear Environmental Engineering Sciences and Radioactive Waste Management (NEESRWM), Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC.

Abstract: Understanding of the behavior and effects of plutonium (Pu) in the environment is an important aspect of developing responsible and effective strategies for remediation and environmental stewardship. This work studies the sorption and uptake of 239Pu by common environmental bacteria, Escherichia coli DH10β and Pseudomonas putida KT-2440. Plutonium was directly incorporated into growth media prior to inoculation (0. Read More

View Article and Full-Text PDF
November 2021

Effect of graphene oxide flakes size and number of layers on photocatalytic hydrogen production.

Sci Rep 2021 Aug 5;11(1):15969. Epub 2021 Aug 5.

Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, 61-614, Poznan, Poland.

The present study explored the correlation between the photocatalytic activity toward hydrogen production of the graphene-based materials and graphene oxide (GO) morphology. In this work we applied the technique based on the combination of time-dependent sonication and iterative centrifugation cascades, which were designed to achieve nanosheets size and the number of layers selection. First such obtained GO dispersions were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and optical spectroscopy. Read More

View Article and Full-Text PDF

Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.

Front Vet Sci 2021 19;8:668158. Epub 2021 Jul 19.

Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Barcelona, Spain.

RNA-Seq data from human semen suggests that the study of the sperm transcriptome requires the previous elimination from the ejaculates of somatic cells carrying a larger load of RNA. Semen purification is also carried to study the sperm transcriptome in other species including swine and it is often done by density gradient centrifugation to obtain viable spermatozoa from fresh ejaculates or artificial insemination doses, thereby limiting the throughput and remoteness of the samples that can be processed in one study. The aim of this work was to evaluate the impact of purification with density gradient centrifugation by BoviPure on porcine sperm. Read More

View Article and Full-Text PDF

Serum index rules prevent risk of analysing uncentrifuged tubes on automated biochemistry analysers.

Scand J Clin Lab Invest 2021 Aug 4:1-6. Epub 2021 Aug 4.

Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

Around 1.5% of the total clinical biochemistry tests performed in laboratories are affected by preanalytical errors. Large, automated chemistry analysers prevent errors and interference by using control systems such as spectrophotometric measurements to evaluate serum indices, i. Read More

View Article and Full-Text PDF

Quantitation of Plasma Membrane Drug Transporters in Kidney Tissue and Cell Lines Using a Novel Proteomic Approach Enabled a Prospective Prediction of Metformin Disposition.

Drug Metab Dispos 2021 Oct 30;49(10):938-946. Epub 2021 Jul 30.

Drug Metabolism and Pharmacokinetics, AbbVie Inc., North Chicago, Illinois

The successful prospective incorporation of in vitro transporter kinetics in physiologically based pharmacokinetic (PBPK) models to describe drug disposition remains challenging. Although determination of scaling factors to extrapolate in vitro to in vivo transporter kinetics has been facilitated by quantitative proteomics, no robust assessment comparing membrane recoveries between different cells/tissues has been made. HEK293 cells overexpressing OCT2, MATE1, and MATE2K or human kidney cortex were homogenized and centrifuged to obtain the total membrane fractions, which were subsequently subjected to liquid-liquid extraction followed by centrifugation and precipitation to isolate plasma membrane fractions. Read More

View Article and Full-Text PDF
October 2021

Techno-economic assessment of a sustainable and cost-effective bioprocess for large scale production of polyhydroxybutyrate.

Chemosphere 2021 Dec 30;284:131371. Epub 2021 Jun 30.

Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India. Electronic address:

The rapid depletion of crude-oil resource which sustains a conventional petroleum refinery together with its environmental impact has led to the search for more sustainable alternatives. In this context, biorefinery serves to fulfil the aim by utilizing waste resources. Hence, this study focused on techno-economic assessment of PHB production at large scale from waste carob pods in a closed-loop biorefinery setup. Read More

View Article and Full-Text PDF
December 2021

Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples.

Biomed Res Int 2021 8;2021:5585148. Epub 2021 Jul 8.

Department of Hematology, Aalborg University Hospital, Denmark.

DNA released from cells into the peripheral blood is known as cell-free DNA (cfDNA), representing a promising noninvasive source of biomarkers that could be utilized to manage Diffuse Large B-Cell Lymphoma (DLBCL), among other diseases. The procedure for purification and handling of cfDNA is not yet standardized, and various preanalytical variables may affect the yield and analysis of cfDNA, including the purification kits, blood collection tubes, and centrifugation regime. Therefore, we aimed to investigate the impact of these preanalytical variables on the yield of cfDNA by comparing three different purification kits DNeasy Blood & Tissue Kit (Qiagen), QIAamp Circulating Nucleic Acid Kit (Qiagen), and Quick-cfDNA Serum & Plasma Kit (Zymo Research). Read More

View Article and Full-Text PDF
September 2021

[Preparation and in vitro evaluation of forsythoside A-loaded exosomes].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(11):2824-2829

School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.

A drug delivery system of forsythoside A-loaded exosomes(FTA-Exos) with high biocompatibility and low immunogenicity was established to investigate its impact on the migration of human lung epithelial adenocarcinoma A549 cells. The exosomes from A549 cells were extracted and purified by ultra-high speed centrifugation and ultrafiltration. FTA-Exos were prepared by ultrasonic incubation, and characterized by particle size analysis, transmission electron microscopy, and Western blot assay. Read More

View Article and Full-Text PDF

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor.

J Vis Exp 2021 06 29(172). Epub 2021 Jun 29.

School of Biological Sciences, Illinois State University;

The exposure of living organisms to environmental and cellular stresses often causes disruptions in protein homeostasis and can result in protein aggregation. The accumulation of protein aggregates in bacterial cells can lead to significant alterations in the cellular phenotypic behavior, including a reduction in growth rates, stress resistance, and virulence. Several experimental procedures exist for the examination of these stressor-mediated phenotypes. Read More

View Article and Full-Text PDF

Exosomes miR-15a promotes nucleus pulposus-mesenchymal stem cells chondrogenic differentiation by targeting MMP-3.

Cell Signal 2021 Oct 10;86:110083. Epub 2021 Jul 10.

Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213003, China. Electronic address:

The physiology of the nucleus pulposus (NP) in intervertebral disc degeneration (IVD) has been studied widely. However, interactions involving nucleus pulposus -mesenchymal stem cells (NP-MSCs) are less understood. MicroRNA 15a (miR-15a) is known to target and modulate genes involved in cellular proliferation and apoptosis. Read More

View Article and Full-Text PDF
October 2021

Impact of centrifugation on thrombin generation in healthy subjects and in patients treated with direct oral anticoagulants.

Int J Lab Hematol 2021 Jul 12. Epub 2021 Jul 12.

Université catholique de Louvain, CHU UCL Namur, Namur Thrombosis and Hemostasis Center (NTHC), Hematology Laboratory, Yvoir, Belgium.

Introduction: Double centrifugation before freezing is recommended before thrombin generation assays (TGA). However, this procedure is not mandatory for routine hemostasis tests, precluding the use of these samples for TGA. The aim of this study is to assess the impact of single and double centrifugation on TGA performed on frozen samples from healthy volunteers (HVs) and patients receiving direct oral anticoagulants (DOACs). Read More

View Article and Full-Text PDF

Graphene-Assisted Synthesis of 2D Polyglycerols as Innovative Platforms for Multivalent Virus Interactions.

Adv Funct Mater 2021 May 26;31(22):2009003. Epub 2021 Mar 26.

Department of Chemistry Faculty of Science Lorestan University Khorramabad Iran.

2D nanomaterials have garnered widespread attention in biomedicine and bioengineering due to their unique physicochemical properties. However, poor functionality, low solubility, intrinsic toxicity, and nonspecific interactions at biointerfaces have hampered their application in vivo. Here, biocompatible polyglycerol units are crosslinked in two dimensions using a graphene-assisted strategy leading to highly functional and water-soluble polyglycerols nanosheets with 263 ± 53 nm and 2. Read More

View Article and Full-Text PDF

[Applications of separation technology in novel coronavirus research, epidemic prevention and detection].

Se Pu 2021 Jul;39(7):679-685

School of Life Science, Beijing Institute of Technology, "Molecular Medicine and Biological Diagnosis" Key Laboratory of Ministry of Industry and Information Technology, Beijing 100081, China.

The novel coronavirus disease 2019 (COVID-19) outbreak has brought to light unprecedented challenges to global public health security. Researchers have devoted their efforts to in-depth research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to bring the epidemic under control as rapidly as possible. Among the many areas of burgeoning SARS-CoV-2 related research, various analytical technologies have been applied to the advancement of virus detection, and development of vaccines and innovative therapies. Read More

View Article and Full-Text PDF

[Determination of nine -nitrosamines in animal derived foods by QuEChERS-isotope dilution combined with gas chromatography-tandem mass spectrometry].

Se Pu 2021 Jan;39(1):96-103

Fisheries College of Jimei University, Xiamen 361021, China;2. Xiamen Customs Technology Center, Xiamen 361000, China.

In this study, a comprehensive analytical method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of nine -nitrosamines in animal derived foods. There are many kinds of -nitrosamines in foods that are harmful to human health. However, the national standard GB 5009. Read More

View Article and Full-Text PDF
January 2021

[Size exclusion-reverse liquid column chromatography-mass spectrometry and its application in the identification of post-translationally modified proteins in rat kidney].

Se Pu 2021 Jan;39(1):87-95

Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China.

Proteomics is an emerging field that has been shown to play a crucial role in unveiling the mechanisms underlying physiological and pathological processes, and liquid chromatography-mass spectrometry (LC-MS) is one of the most important methods employed in this field. However, in complex biological systems, such as eukaryotes, it is challenging to perform a comprehensive and unbiased proteome analysis due to the high complexity of biological samples and enormous differences in sample contents. For example, post-translational modifications (PTMs) in proteins are imperative for cell signaling, but post-translationally modified proteins account for about 1% of the total proteins in a single cell, making their identification extremely difficult. Read More

View Article and Full-Text PDF
January 2021

Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients.

Sci Rep 2021 06 30;11(1):13581. Epub 2021 Jun 30.

Department of Sports Medicine, Rehabilitation and Disease Prevention, University of Mainz, Albert-Schweitzer Str. 22, 55128, Mainz, Germany.

Circulating cell-free DNA (cfDNA) has been investigated as a screening tool for many diseases. To avoid expensive and time-consuming DNA isolation, direct quantification PCR assays can be established. However, rigorous validation is required to provide reliable data in the clinical and non-clinical context. Read More

View Article and Full-Text PDF

Relationships between inflammatory and metabolic markers, exercise, and body composition in young individuals.

J Clin Transl Res 2021 Jun 14;7(3):289-296. Epub 2021 May 14.

Department of Biology, University of La Verne, La Verne, California.

Background And Aims: Physical exercise may help combat disease and elicits a possible "protective" anti-inflammatory effect on the body. Inflammatory cytokines, C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-α (TNFα), along with transcription factor, nuclear factor-kappa B (NFB) in young (n=16, 21.1±2. Read More

View Article and Full-Text PDF

Impact of sample collection on prokaryotic and eukaryotic diversity of niche environments of the oil-sand mining impacted Athabasca River.

Can J Microbiol 2021 Jun 25:1-14. Epub 2021 Jun 25.

Environment and Climate Change Canada, Canada Centre for Inland Waters, Burlington, ON, Canada.

Microbial communities are an important aspect of overall riverine ecology; however, appreciation of the effects of anthropogenic activities on unique riverine microbial niches, and how the collection of these samples affects the observed diversity and community profile is lacking. We analyzed prokaryotic and eukaryotic communities from surface water, biofilms, and suspended load niches along a gradient of oil sands-related contamination in the Athabasca River (Alberta, Canada), with suspended load or particle-associated communities collected either via Kenney Sampler or centrifugation manifold. At the phylum level, different niche communities were highly similar to each other and across locations. Read More

View Article and Full-Text PDF