23 results match your criteria immunostainings fish

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Molecular diagnosis in non-small-cell lung cancer: expert opinion on and testing.

J Clin Pathol 2021 Apr 19. Epub 2021 Apr 19.

Department of Pathology, Hospital Universitario Virgen del Rocío, Sevilla, Spain.

The effectiveness of targeted therapies with tyrosine kinase inhibitors in non-small-cell lung cancer (NSCLC) depends on the accurate determination of the genomic status of the tumour. For this reason, molecular analyses to detect genetic rearrangements in some genes (ie, , , ) have become standard in patients with advanced disease. Since immunohistochemistry is easier to implement and interpret, it is normally used as the screening procedure, while fluorescence in situ hybridisation (FISH) is used to confirm the rearrangement and decide on ambiguous immunostainings. Read More

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Clinicopathological Findings on 28 Cases with XP11.2 Renal Cell Carcinoma.

Pathol Oncol Res 2020 Oct 18;26(4):2123-2133. Epub 2020 Jan 18.

Department of Pathology, University of Szeged, 1 Állomás Street, Szeged, H-6725, Hungary.

Xp11.2 translocation carcinoma is a distinct subtype of renal cell carcinoma characterized by translocations involving the TFE3 gene. Our study included the morphological, immunohistochemical and clinicopathological examination of 28 Xp11. Read More

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October 2020

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species.

Aquat Toxicol 2020 Feb 17;219:105384. Epub 2019 Dec 17.

Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PSE-ENV/SRTE/LECO, Cadarache, 13115, Saint-Paul-lez-Durance, France. Electronic address:

Tritium (H), a radioactive isotope of hydrogen, is ubiquitously present in the environment. In a previous study, we highlighted a mis-regulation of genes involved in muscle contraction, eye transparency and response to DNA damages after exposure of zebrafish embryo-larvae from 3 hpf to 96 hpf at 0.4 and 4 mGy/h of tritiated water (HTO). Read More

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February 2020

A practical guide to optimization in X10 expansion microscopy.

Nat Protoc 2019 03;14(3):832-863

Institute of Science and Technology Austria, Klosterneuburg, Austria.

Expansion microscopy is a relatively new approach to super-resolution imaging that uses expandable hydrogels to isotropically increase the physical distance between fluorophores in biological samples such as cell cultures or tissue slices. The classic gel recipe results in an expansion factor of ~4×, with a resolution of 60-80 nm. We have recently developed X10 microscopy, which uses a gel that achieves an expansion factor of ~10×, with a resolution of ~25 nm. Read More

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Glial Cells in the Fish Retinal Nerve Fiber Layer Form Tight Junctions, Separating and Surrounding Axons.

Front Mol Neurosci 2018 10;11:367. Epub 2018 Oct 10.

Institut für klinische Anatomie und Zellanalytik, Universität Tübingen, Tübingen, Germany.

In the retina of teleost fish, cell addition continues throughout life involving proliferation and axonal growth. To study how this is achieved in a fully functioning retina, we investigated the nerve fiber layer (NFL) of the cichlid fish for components that might regulate the extracellular environment. We hypothesized that growing axons are surrounded by different cell structures than signal conducting axons. Read More

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October 2018

Modulation of Rb phosphorylation and antiproliferative response to palbociclib: the preoperative-palbociclib (POP) randomized clinical trial.

Ann Oncol 2018 08;29(8):1755-1762

Department of Cancer Medicine, Breast Cancer Committee, Gustave Roussy, Villejuif, France; INSERM Unit 981, Gustave Roussy, Villejuif, France.

Background: The cyclin-dependent kinase 4 (CDK4)/6 inhibitor Palbociclib is a new standard treatment in hormone-receptor positive breast cancer patients. No predictive biomarkers have been identified and no pharmacodynamics has properly been described so far.

Patients And Methods: Patients with early-breast cancer were randomized 3 : 1 to oral palbociclib 125 mg daily for 14 days until the day before the surgery versus no treatment. Read More

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Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve.

J Comp Neurol 2017 Oct 23;525(14):2991-3009. Epub 2017 Jun 23.

Department of Biology, University of Konstanz, Konstanz, Germany.

This study explored why lesioned retinal ganglion cell (RGC) axons regenerate successfully in the zebrafish optic nerve despite the presence of Rtn4b, the homologue of the rat neurite growth inhibitor RTN4-A/Nogo-A. Rat Nogo-A and zebrafish Rtn4b possess characteristic motifs (M1-4) in the Nogo-A-specific region, which contains delta20, the most inhibitory region of rat Nogo-A. To determine whether zebrafish M1-4 is inhibitory as rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. Read More

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October 2017

Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure.

Int J Mol Sci 2017 May 7;18(5). Epub 2017 May 7.

Kirchhoff-Institute for Physics, Heidelberg University, Im Neuenheimer Feld 227, 69120 Heidelberg, Germany.

Immunostaining and fluorescence in situ hybridization (FISH) are well established methods for specific labelling of chromatin in the cell nucleus. COMBO-FISH (combinatorial oligonucleotide fluorescence in situ hybridization) is a FISH method using computer designed oligonucleotide probes specifically co-localizing at given target sites. In combination with super resolution microscopy which achieves spatial resolution far beyond the Abbe Limit, it allows new insights into the nano-scaled structure and organization of the chromatin of the nucleus. Read More

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Diffuse leptomeningeal glioneuronal tumor (DLGNT) mimicking Whipple's disease: a case report and literature review.

Childs Nerv Syst 2017 Aug 5;33(8):1411-1414. Epub 2017 Apr 5.

Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Introduction: Diffuse leptomeningeal glioneuronal tumor is a new entity under the neuronal and mixed neuronal-glial tumors in the WHO 2016 updated classification and commonly found in children and adolescents. The initial diagnosis is challenging because of its non-specific radiologic feature and negative CSF cytology analysis. A 17 years male was presented with intractable headache subsequently followed by back pain and joint pain. Read More

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The Brain of the Archerfish : A Nissl-Based Neuroanatomical Atlas and Catecholaminergic/Cholinergic Systems.

Front Neuroanat 2016 11;10:106. Epub 2016 Nov 11.

Graduate School of Systemic Neurosciences and Division of Neurobiology, Department Biology II, Ludwig-Maximilians-University of Munich Munich, Germany.

Over recent years, the seven-spot archerfish () has emerged as a new model for studies in visual and behavioral neuroscience thanks to its unique hunting strategy. Its natural ability to spit at insects outside of water can be used in the laboratory for well controlled behavioral experiments where the fish is trained to aim at targets on a screen. The need for a documentation of the neuroanatomy of this animal became critical as more research groups use it as a model. Read More

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November 2016

Donor-derived stem-cells and epithelial mesenchymal transition in squamous cell carcinoma in transplant recipients.

Oncotarget 2015 Dec;6(39):41497-507

INSERM, UMR_S1165, Paris, F-75010, France.

Background: Skin squamous-cell-carcinoma (SCC), is the main complication in long-term kidney-transplant recipients, and it can include donor-derived cells. Preclinical models demonstrated the involvement of epithelial mesenchymal transition (EMT) in the progression of skin SCC, and the role of Snail, an EMT transcription factor, in cancer stem-cell survival and expansion.Here, we studied stem-cells and EMT expression in SCCs and concomitant actinic keratoses (AK) in kidney-transplant recipients. Read More

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December 2015

Matrix metalloproteinases as promising regulators of axonal regrowth in the injured adult zebrafish retinotectal system.

J Comp Neurol 2016 May 18;524(7):1472-93. Epub 2015 Nov 18.

Laboratory of Neural Circuit Development and Regeneration, Biology Department, KU Leuven, Leuven, Belgium.

Overcoming the failure of axon regeneration in the mammalian central nervous system (CNS) after injury remains a major challenge, which makes the search for proregenerative molecules essential. Matrix metalloproteinases (MMPs) have been implicated in axonal outgrowth during CNS development and show increased expression levels during vertebrate CNS repair. In mammals, MMPs are believed to alter the suppressive extracellular matrix to become more permissive for axon regrowth. Read More

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Chromosome instability predicts progression of premalignant lesions of the larynx.

Pathology 2014 Apr;46(3):216-24

1Department of Otorhinolaryngology and Head and Neck Surgery, GROW-School for Oncology & Developmental Biology, Maastricht University Medical Centre 2Department of Otorhinolaryngology and Head and Neck Surgery, Atrium Medical Centre, Heerlen 3Departments of Molecular Cell Biology 4Pathology 5Epidemiology 6School for Mental Health and Neuroscience 7Department of Methodology and Statistics, Faculty of Health, Medicine and Life Sciences, Maastricht University Medical Centre 8Department of Pathology, University Medical Centre Groningen, The University of Groningen, The Netherlands.

The histopathology of premalignant laryngeal lesions does not provide reliable information on the risk of malignant transformation, hence we examined new molecular markers which can easily be implemented in clinical practice. Dual-target fluorescence in situ hybridisation (FISH) for chromosome 1 and 7 centromeres was performed on tissue sections of laryngeal premalignancies in 69 patients. Chromosome instability was indicated by numerical imbalances and/or polysomy for chromosomes 1 and 7. Read More

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The toxicity of silver nanoparticles to zebrafish embryos increases through sewage treatment processes.

Ecotoxicology 2013 Oct 22;22(8):1264-77. Epub 2013 Aug 22.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074, Aachen, Germany.

Silver nanoparticles (AgNPs) are widely believed to be retained in the sewage sludge during sewage treatment. The AgNPs and their derivatives, however, re-enter the environment with the sludge and via the effluent. AgNP were shown to occur in surface water, while evidence of a potential toxicity of AgNPs in aquatic organisms is growing. Read More

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October 2013

Integration of HPV6 and downregulation of AKR1C3 expression mark malignant transformation in a patient with juvenile-onset laryngeal papillomatosis.

PLoS One 2013 20;8(2):e57207. Epub 2013 Feb 20.

Jean-Uhrmacher-Institute for Otorhinolaryngological Research, University of Cologne, Cologne, Germany.

Juvenile-onset recurrent respiratory papillomatosis (RRP) is associated with low risk human papillomavirus (HPV) types 6 and 11. Malignant transformation has been reported solely for HPV11-associated RRP in 2-4% of all RRP-cases, but not for HPV6. The molecular mechanisms in the carcinogenesis of low risk HPV-associated cancers are to date unknown. Read More

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The classification of motor neuron defects in the zebrafish embryo toxicity test (ZFET) as an animal alternative approach to assess developmental neurotoxicity.

Neurotoxicol Teratol 2012 Jul 24;34(4):413-24. Epub 2012 Apr 24.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen and Schmallenberg, Germany.

Rodents are widely used to test the developmental neurotoxicity potential of chemical substances. The regulatory test procedures are elaborate and the requirement of numerous animals is ethically disputable. Therefore, non-animal alternatives are highly desirable, but appropriate test systems that meet regulatory demands are not yet available. Read More

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EZH2, Ki-67 and MCM7 are prognostic markers in prostatectomy treated patients.

Int J Cancer 2008 Feb;122(3):595-602

Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.

The aim of the study was to evaluate the prognostic value of Ki-67, EZH2, MCM7 and EIF3S3 in prostatectomy treated patients. A retrospective population-based material of 249 radical prostatectomy specimens on tissue microarrays was utilized. The median follow-up of the patients was approximately 5. Read More

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February 2008

Cellular resolution expression profiling using confocal detection of NBT/BCIP precipitate by reflection microscopy.

Biotechniques 2007 Jun;42(6):751-5

Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

The determination of gene expression patterns in three dimensions with cellular resolution is an important goal in developmental biology. However the most sensitive, efficient, and widely used staining technique for whole-mount in situ hybridization (WMISH), nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) precipitation by alkaline phosphatase, could not yet be combined with the most precise, high-resolution detection technique, confocal laser-scanning microscopy (CLSM). Here we report the efficient visualization of the NBT/BCIP precipitate using confocal reflection microscopy for WMISH samples of Drosophila, zebrafish, and the marine annelid worm, Platynereis dumerilii. Read More

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HER2 evaluation using the novel rabbit monoclonal antibody SP3 and CISH in tissue microarrays of invasive breast carcinomas.

J Clin Pathol 2007 Sep 8;60(9):1001-5. Epub 2006 Dec 8.

IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal.

Background: Laboratory methods for HER2 assessment currently include immunohistochemical (IHC) methods (measuring protein overexpression) and fluorescence in situ hybridisation (FISH) (measuring gene amplification). The measure of HER2 protein by IHC is usually assessed by the mouse monoclonal antibody CB11, and polyclonal antibodies (Herceptest) directed against the internal portion of the receptor. Recently, chromogenic in situ hybridisation (CISH), in which HER2 is detected by a peroxidase reaction and the gene amplification can be determined by regular bright-field microscopy, has emerged as an alternative to FISH. Read More

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September 2007

Selecting antibodies to detect HER2 overexpression by immunohistochemistry in invasive mammary carcinomas.

Appl Immunohistochem Mol Morphol 2006 Mar;14(1):103-8

Department of Anatomic Pathology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

There is an increasing clinical demand for HER2 analysis in breast cancer, especially since the release of trastuzumab. The authors assessed the ability of immunohistochemistry to detect HER2 overexpression in invasive mammary carcinomas (IMC) using five antibodies. Paraffin-embedded samples of 86 IMCs (T2N0) were used to compare the immunohistochemical overexpression of HER2 using two polyclonal antibodies (HercepTest [DAKO] and A0485 [DAKO]) and three monoclonal antibodies (CB11 from two different laboratories, Biogenex and Novocastra, and 4D5 [Genentech]). Read More

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A dopamine- and protein kinase A-dependent mechanism for network adaptation in retinal ganglion cells.

J Neurosci 2001 Nov;21(21):8624-35

Section of Neurobiology, Physiology, and Behavior, University of California, Davis, California 95616, USA.

Vertebrates can detect light intensity changes in vastly different photic environments, in part, because postreceptoral neurons undergo "network adaptation." Previous data implicated dopaminergic, cAMP-dependent inhibition of retinal ganglion cells in this process yet left unclear how this occurs and whether this occurs in darkness versus light. To test for light- and dopamine-dependent changes in ganglion cell cAMP levels in situ, we immunostained dark- and light-adapted retinas with anti-cAMP antisera in the presence and absence of various dopamine receptor ligands. Read More

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November 2001

E587 antigen is upregulated by goldfish oligodendrocytes after optic nerve lesion and supports retinal axon regeneration.

Glia 1998 Jul;23(3):257-70

Division of Biology, University of Konstanz, Germany.

The properties of glial cells in lesioned nerves contribute quite substantially to success or failure of axon regeneration in the CNS. Goldfish retinal axons regenerate after optic nerve lesion (ONS) and express the L1-like cell adhesion protein E587 antigen on their surfaces. Goldfish oligodendrocytes in vitro also produce E587 antigen and promote growth of both fish and rat retinal axons. Read More

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Coexistence of corticotropin-releasing factor-like immunoreactivity and vasotocin in perikarya of the preoptic nucleus in the eel.

Gen Comp Endocrinol 1988 Apr;70(1):41-8

Laboratoire de Physiologie, Institut Océanographique, Paris, France.

A corticotropin-releasing factor (CRF)-like system has been identified in eels (Anguilla anguilla, A. rostrata, and A. japonica) with immunocytochemical techniques. Read More

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