5,959 results match your criteria immunostaining vitro

An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual.

Stem Cell Res 2021 Apr 8;53:102336. Epub 2021 Apr 8.

Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan 410008, China. Electronic address:

We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. Read More

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Metalloelastase-12 is involved in the temporomandibular joint inflammatory response as well as cartilage degradation by aggrecanases in STR/Ort mice.

Biomed Rep 2021 Jun 1;14(6):51. Epub 2021 Apr 1.

Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920-8640, Japan.

Temporomandibular joint dysfunction (TMJD) is characterised by clinical symptoms involving both the masticatory muscles and the temporomandibular joint (TMJ). Disc internal derangement and osteoarthritis (OA) are the most common forms of TMJD. Currently, the molecular process associated with degenerative changes in the TMJ is unclear. Read More

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Identification of the canonical and noncanonical role of miR-143/145 in estrogen-deficient bone loss.

Theranostics 2021 13;11(11):5491-5510. Epub 2021 Mar 13.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

Postmenopausal-induced bone loss is mainly caused by declining core transcription factors (TFs) of bone mesenchymal stem cells (BMSCs), but little is known about how miRNAs regulate chromatin structure remodeling of TFs gene to maintain BMSCs function in bone homeostasis. We examined the serum, salivary and bone samples from Pre- and Post-menopause women by paired analysis and confirmed canonical ceRNA role of MIR143HG and miR-143/145 complexes in cytoplasm and noncanonical role for SOX2 transcription in nucleus (FISH, qRT-PCR, immunostaining, Luciferase assays and ChIP). Moreover, we took advantage of transgenic mice under OVX-induced osteoporosis, studying the and effect of miR-143/145 deletion on BMSCs function and bone homeostasis. Read More

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Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly.

Theranostics 2021 5;11(11):5197-5213. Epub 2021 Mar 5.

College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China.

Zearalenone (ZEN), a pollutant in our daily diet, seriously threatens the reproductive health of humans and animals. The primordial follicle (PF) assembly in the mouse occurs during the perinatal period, which determines the whole ovarian reserve in reproductive lifespan. In the current investigation, we administered ZEN orally to perinatal mice from 16. Read More

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Scaffold-Free Engineering of Human Cartilage Implants.

Cartilage 2021 Apr 15:19476035211007923. Epub 2021 Apr 15.

Department of Immunology, Oslo University Hospital, Oslo, Norway.

Objective: Despite new strategies in tissue engineering, cartilage repair remains a major challenge. Our aim is to treat patients with focal lesions of articular cartilage with autologous hyaline cartilage implants using a scaffold-free approach. In this article, we describe experiments to optimize production of scaffold-free cartilage discs. Read More

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Antisense oligonucleotides targeting alternative splicing of Nrcam exon 10 suppress neurite outgrowth of ganglion sensory neurons in vitro.

Neuroreport 2021 May;32(7):548-554

Department of Physiology and Pathophysiology, School of Basic Medical Sciences.

Neuron-glial-related cell adhesion molecule (NrCAM) is a neuronal cell adhesion molecule that has been shown to be involved in several cellular processes in the peripheral nervous system, including neurite outgrowth. We recently reported that alternative splicing of Nrcam mRNA at exon 10 in the dorsal root ganglion (DRG) contributes to the peripheral mechanism of neuropathic pain. Specially, Nrcam antisense oligonucleotides (ASO) targeting Nrcam exon 10, attenuated neuropathic pain hypersensitivities in mice. Read More

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3D-Printed Design of Antibiotic-Releasing Esophageal Patches for Animicrobial Activity Prevention.

Tissue Eng Part A 2021 Apr 13. Epub 2021 Apr 13.

Seoul National University Hospital, 58927, Department of Otorhinolaryngology - Head and Neck Surgery, 101 Daehakro, Jongno-gu, Seoul, College of Medicine, Jongno-gu, Korea (the Republic of), 110-744;

Pharyngo-esophageal defects can cause exposure to various bacterial flora and severe inflammation. We fabricated a biodegradable polycaprolactone (PCL) patch composed of both thin film and 3D printed lattice, and then investigated the efficacy of pharyngo-esophageal reconstruction by using 3D-printed antibiotic-releasing PCL patches that inhibited early inflammation by sustained tetracycline (TCN) release from both thin PCL films and printed rods implanted in esophageal partial defects. PCL was 3D printed in lattice form on a presolution casted PCL thin film at approximately 100 µm resolution. Read More

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Inhibitory neurons exhibit high controlling ability in the cortical microconnectome.

PLoS Comput Biol 2021 Apr 8;17(4):e1008846. Epub 2021 Apr 8.

Graduate Schools of Medicine, Kyoto University, Kyoto, Japan.

The brain is a network system in which excitatory and inhibitory neurons keep activity balanced in the highly non-random connectivity pattern of the microconnectome. It is well known that the relative percentage of inhibitory neurons is much smaller than excitatory neurons in the cortex. So, in general, how inhibitory neurons can keep the balance with the surrounding excitatory neurons is an important question. Read More

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Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6.

Oxid Med Cell Longev 2021 20;2021:6628957. Epub 2021 Mar 20.

South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.

Background: Doxorubicin (DOX) is a commonly used chemotherapeutic drug but is limited in clinical applications by its cardiotoxicity. acupoint (PC6) is a well-recognized acupoint for the treatment of cardiothoracic disease. However, whether acupuncture at PC6 could be effective in preventing DOX-induced cardiotoxicity is still unknown. Read More

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The Expression and Function of Human Ribonuclease 4 in the Kidney and Urinary Tract.

Am J Physiol Renal Physiol 2021 Apr 5. Epub 2021 Apr 5.

Pediatrics, Nationwide Children's Hospital, United States.

Antimicrobial peptides are essential host defense mechanisms that prevent urinary tract infections. Recent studies demonstrate that peptides in the Ribonuclease A Superfamily have antimicrobial activity against uropathogens and protect the urinary tract from uropathogenic Escherichia coli (UPEC). Little is known is about the antibacterial function or expression of Ribonuclease 4 in the human urinary tract. Read More

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Claudin-1 down-regulation in the prostate is associated with aging and increased infiltration of inflammatory cells in BPH.

Am J Clin Exp Urol 2021 15;9(1):53-64. Epub 2021 Feb 15.

Department of Urology, University of Pittsburgh School of Medicine Pittsburgh, PA, USA.

Introduction And Objective: Benign prostatic hyperplasia (BPH) is an age-related disease that is frequently associated with chronic prostatic inflammation. In previous studies, we detected the presence of PSA protein in the stroma of BPH nodules and down-regulation of junction proteins E-cadherin and claudin-1. Transmission electron microscopy (TEM) imaging showed a decrease in tight junctions suggesting the luminal epithelial barrier in BPH tissues may be compromised. Read More

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February 2021

NF-κB activation in retinal microglia is involved in the inflammatory and neovascularization signaling in laser-induced choroidal neovascularization in mice.

Exp Cell Res 2021 Mar 31;403(1):112581. Epub 2021 Mar 31.

Department of Ophthalmology, School of Medicine, Sapporo Medical University, Sapporo, Japan.

Purpose: To evaluate Nuclear Factor NF-κB (NF-κB) signaling on microglia activation, migration, and angiogenesis in laser-induced choroidal neovascularization (CNV).

Methods: Nine-week-old C57BL/6 male mice were randomly assigned to IMD-0354 treated or untreated groups (5 mice, 10 eyes per group). CNV was induced with a 532-nm laser. Read More

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Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase.

Int J Mol Sci 2021 Mar 29;22(7). Epub 2021 Mar 29.

Research Institute of Sciences & Engineering, University of Sharjah, Sharjah 27272, United Arab Emirates.

It is evident that depletion of interstitial cells and dysfunction of nitric oxide (NO) pathways are key players in development of several gastrointestinal (GI) motility disorders such as diabetic gastroparesis (DGP). One of the main limitations of DGP research is the lack of isolation methods that are specific to interstitial cells, and therefore conducting functional studies is not feasible. The present study aims (i) to differentiate telomerase transformed mesenchymal stromal cells (iMSCs) into platelet-derived growth factor receptor-α-positive cells (PDGFRα-positive cells) using connective tissue growth factor (CTGF) and L-ascorbic acids; (ii) to investigate the effects of NO donor and inhibitor on the survival rate of differentiated PDGFRα-positive cells; and (iii) to evaluate the impact of increased glucose concentrations, mimicking diabetic hyperglycemia, on the gene expression of neuronal nitric oxide synthase (nNOS). Read More

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Interferon regulatory factor 6 correlates with the progression of non-small cell lung cancer and can be regulated by miR-320.

J Pharm Pharmacol 2021 Mar;73(5):682-691

Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin Province, P.R. China.

Objectives: The expression of interferon regulatory factor 6 (IRF6) has been reported in several cancer types, but its roles underlying the progression of lung cancer have not been detailedly investigated.

Methods: The pairs of lung cancer tissues and para-carcinoma tissues and The Cancer Genome Atlas database were collected to detect IRF6 expression. Cell counting kit-8, transwell and terminal-deoxynucleoitidyl transferase-mediated nick end labelling assays were used to evaluate cell proliferation, migration and apoptosis. Read More

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Dicer increases the indication for trastuzumab treatment in gastric cancer patients via overexpression of human epidermal growth factor receptor 2.

Sci Rep 2021 Mar 26;11(1):6993. Epub 2021 Mar 26.

Department of Immunology and Rheumatology, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang, 050011, People's Republic of China.

The low proportion of gastric cancer (GC) patients with high HER2 expression limits the clinical application of trastuzumab, a humanized epidermal growth factor receptor 2 (HER2) antibody targeting for GC treatment. We found that Dicer was positively correlated with HER2 expression in GC tissue by immunostaining as well as induce HER2 overexpression without increasing invasiveness of GC cell. In addition, both the growth of GC referring to cell proliferation, invasion, migration and apoptosis was inhibited by Dicer overexpression. Read More

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The difficulty to model Huntington's disease in vitro using striatal medium spiny neurons differentiated from human induced pluripotent stem cells.

Sci Rep 2021 Mar 25;11(1):6934. Epub 2021 Mar 25.

Institute of Physiology, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany.

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expanded polyglutamine repeat in the huntingtin gene. The neuropathology of HD is characterized by the decline of a specific neuronal population within the brain, the striatal medium spiny neurons (MSNs). The origins of this extreme vulnerability remain unknown. Read More

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The hepatokine fetuin-A disrupts functional maturation of pancreatic beta cells.

Diabetologia 2021 Mar 25. Epub 2021 Mar 25.

Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Center Munich at the Eberhard Karls University of Tuebingen (IDM), Tuebingen, Germany.

Aims/hypothesis: Neonatal beta cells carry out a programme of postnatal functional maturation to achieve full glucose responsiveness. A partial loss of the mature phenotype of adult beta cells may contribute to a reduction of functional beta cell mass and accelerate the onset of type 2 diabetes. We previously found that fetuin-A, a hepatokine increasingly secreted by the fatty liver and a determinant of type 2 diabetes, inhibits glucose-stimulated insulin secretion (GSIS) of human islets. Read More

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FOXD1 regulates cell division in clear cell renal cell carcinoma.

BMC Cancer 2021 Mar 24;21(1):312. Epub 2021 Mar 24.

The Rogosin Institute, 310 East 67th Street, New York, NY, 10065, USA.

Background: Forkhead transcription factors control cell growth in multiple cancer types. Foxd1 is essential for kidney development and mitochondrial metabolism, but its significance in renal cell carcinoma (ccRCC) has not been reported.

Methods: Transcriptome data from the TCGA database was used to correlate FOXD1 expression with patient survival. Read More

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Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion.

Cancer Biol Med 2021 Mar 23. Epub 2021 Mar 23.

Department of Biology, Huck Institutes of Life Sciences, Pennsylvania State University, University Park, PA 16802, USA.

Objective: Glioblastoma (GBM) is the most prevalent and aggressive adult primary cancer in the central nervous system. Therapeutic approaches for GBM treatment are under intense investigation, including the use of emerging immunotherapies. Here, we propose an alternative approach to treat GBM through reprogramming proliferative GBM cells into non-proliferative neurons. Read More

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Ultrasound-mediated augmented exosome release from astrocytes alleviates amyloid-β-induced neurotoxicity.

Theranostics 2021 25;11(9):4351-4362. Epub 2021 Feb 25.

Paul C.Lauterbur research center for biomedical imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People's Republic of China. 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, 518055, China.

Extracellular vesicles, including exosomes, are secreted by a variety of cell types in the central nervous system. Exosomes play a role in removing intracellular materials from the endosomal system. Alzheimer's disease (AD) is caused by an overproduction or reduced amyloid-beta (Aβ) peptide clearance. Read More

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February 2021

Eosinophil ETosis-mediated release of galectin-10 in eosinophilic granulomatosis with polyangiitis.

Arthritis Rheumatol 2021 Mar 22. Epub 2021 Mar 22.

National Hospital Organization Sagamihara National Hospital, Clinical Research Center, Kanagawa, Japan.

Objective: Eosinophils are tissue-dwelling immune cells. Accumulating evidence indicates that a type of cell death termed ETosis is an important cell fate involved in the pathophysiology of inflammatory diseases. Although the critical role of eosinophils, in eosinophilic granulomatosis with polyangiitis (EGPA, formerly Churg-Strauss syndrome) is well established, the presence of eosinophil ETosis (EETosis) is poorly understood. Read More

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Qingda granule exerts neuroprotective effects against ischemia/reperfusion-induced cerebral injury via lncRNA GAS5/miR-137 signaling pathway.

Int J Med Sci 2021 6;18(7):1687-1698. Epub 2021 Feb 6.

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, 1 Qiuyang Road, Minhou Shangjie, Fuzhou 350122, China.

Ischemic stroke is the second leading cause of death and disability worldwide, which needs to develop new pharmaceuticals for its prevention and treatment. Qingda granule (QDG), a traditional Chinese medicine formulation, could improve angiotensin II-induced brain injury and decrease systemic inflammation. In this study, we aimed to evaluate the neuroprotective effect of QDG against ischemia/reperfusion-induced cerebral injury and illustrate the potential mechanisms. Read More

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February 2021

Beclin-1 improves mitochondria-associated membranes in the heart during endotoxemia.

FASEB Bioadv 2021 Mar 25;3(3):123-135. Epub 2021 Jan 25.

Department of Surgery University of Texas Southwestern Medical Center Dallas TX USA.

Mitochondria-associated membranes (MAMs) are essential to mitochondria. This study was to determine whether endotoxemia rearranges MAMs in the heart, and whether Beclin-1 regulates this process. Wild-type mice and mice with a cardiac-specific overexpression of Beclin-1 (-), or a heterozygous knockout of Beclin-1 () were given lipopolysaccharide (LPS) challenge. Read More

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Neuroprotection of Exendin-4 by Enhanced Autophagy in a Parkinsonian Rat Model of α-Synucleinopathy.

Neurotherapeutics 2021 Mar 15. Epub 2021 Mar 15.

Department of Neurology & National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China.

Glucagon-like peptide-1 (GLP-1) receptor stimulation ameliorates parkinsonian motor and non-motor deficits in both experimental animals and patients; however, the disease-modifying mechanisms of GLP-1 receptor activation have remained unknown. The present study investigated whether exendin-4 (a GLP-1 analogue) can rescue motor deficits and exert disease-modifying effects in a parkinsonian rat model of α-synucleinopathy. This model was established by unilaterally injecting AAV-9-A53T-α-synuclein into the right substantia nigra pars compacta, followed by 4 or 8 weeks of twice-daily intraperitoneal injections of exendin-4 (5 μg/kg/day) starting at 2 weeks after AAV-9-A53T-α-synuclein injections. Read More

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Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action.

Am J Chin Med 2021 10;49(3):677-703. Epub 2021 Mar 10.

Clinical and Health Sciences, University of South Australia, Adelaide 5000, South Australia, Australia.

To investigate the therapeutic efficacy of Scutellarin (SCU) on neurite growth and neurological functional recovery in neonatal hypoxic-ischemic (HI) rats. Primary cortical neurons were cultured to detect the effect of SCU on cell viability of neurons under oxygen-glucose deprivation (OGD). Double immunofluorescence staining of Tuj1 and TUNEL then observed the neurite growth and cell apoptosis and double immunofluorescence staining of NEUN and TUNEL was performed to examine the neuronal apoptosis and cell apoptosis in brain tissues after HI . Read More

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Lactate Dehydrogenase A as a Potential New Biomarker for Thyroid Cancer.

Endocrinol Metab (Seoul) 2021 Feb 24;36(1):96-105. Epub 2021 Feb 24.

Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul, Korea.

Background: Several cancers show increased levels of lactate dehydrogenase A (LDHA), which are associated with cancer progression. However, it remains unclear whether LDHA levels are associated with papillary thyroid cancer (PTC) aggressiveness or with the presence of the PTC prognostic marker, the BRAFV600E mutation. This study aimed to evaluate the potential of LDHA as a PTC prognostic marker. Read More

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February 2021

Long non-coding RNA THRIL inhibits miRNA-24-3p to upregulate neuropilin-1 to aggravate cerebral ischemia-reperfusion injury through regulating the nuclear factor κB p65 signaling.

Aging (Albany NY) 2021 03 6;13(6):9071-9084. Epub 2021 Mar 6.

Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

Purpose: The aim of this study was to investigate the role of the tumor necrosis factor and HNRNPL related immunoregulatory long non-coding RNA (THRIL) in cerebral ischemia-reperfusion injury.

Methods: A rat middle cerebral artery occlusion/ischemia-reperfusion (MCAO/IR) model and an oxygen glucose deprivation/reoxygenation (OGD/R) cell model were constructed. THRIL was knocked down using siTHRIL. Read More

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Oxidatively Modified LDL Suppresses Lymphangiogenesis via CD36 Signaling.

Antioxidants (Basel) 2021 Feb 23;10(2). Epub 2021 Feb 23.

Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.

Arterial accumulation of plasma-derived LDL and its subsequent oxidation contributes to atherosclerosis. Lymphatic vessel (LV)-mediated removal of arterial cholesterol has been shown to reduce atherosclerotic lesion formation. However, the precise mechanisms that regulate LV density and function in atherosclerotic vessels remain to be identified. Read More

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February 2021

Regulation and function of leptin during ovarian follicular development in cows.

Anim Reprod Sci 2021 Apr 7;227:106689. Epub 2021 Jan 7.

ReproPel, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil. Electronic address:

Although it is well documented that leptin signals the body nutritional status to the brain, mechanisms of leptin regulation at the ovary are not well understood. This study was conducted to determine whether there was leptin and the receptor for leptin (LEPR) in cattle ovarian follicles and to investigate potential actions of leptin on follicular growth in vivo and on regulation of granulosa cell functions in vitro. There was leptin and LEPR in granulosa and theca cells of dominant and subordinate follicles, with greater immunostaining for leptin in granulosa cells of subordinate follicles. Read More

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Hesperetin promotes DOT1L degradation and reduces histone H3K79 methylation to inhibit gastric cancer metastasis.

Phytomedicine 2021 Apr 10;84:153499. Epub 2021 Feb 10.

Quzhou Hospital, Zhejiang University School of Medicine, Quzhou 324000, China; Department of Core Facility, Quzhou People's Hospital, Quzhou 324000, China; Zhejiang University School of Medicine, Hangzhou 310058, China; Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou 324000, China. Electronic address:

Background: There have been many researches on the effects of flavonoids on tumor treatment or adjuvant therapy, but there are few studies revealing their epigenetic effect on tumors. Hesperetin is a common citrus flavanone widely distributed among citrus fruits. The role of hesperetin in gastric cancer metastasis is unclear. Read More

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