207,334 results match your criteria immunosorbent assay

How to interpret and use COVID-19 serology and immunology tests.

Clin Microbiol Infect 2021 May 8. Epub 2021 May 8.

Institute of Laboratory Medicine, Universities of Giessen and Marburg Lung Centre (UGMLC), Philipps University Marburg, German Centre for Lung Research (DZL) Marburg, Germany.

Background: Although molecular tests are considered the gold standard for coronavirus disease 2019 (COVID-19) diagnostics, serological and immunological tests may be useful in specific settings.

Objectives: This review summarises the underlying principles and performance of COVID-19 serological and immunological testing.

Sources: Selected peer-reviewed publications on COVID-19 related serology and immunology published between December 2019 and March 2021. Read More

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Senescence-associated secretory phenotype and activation of NF-κB in splenocytes of old mice exposed to irradiation at a young age.

Dev Comp Immunol 2021 May 8:104124. Epub 2021 May 8.

Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan. Electronic address:

DNA damage-induced cellular senescence is involved in aging. We reported previously that p53 mice subjected to irradiation at a young age exhibited an increased number of splenic lymphocytes in the S and G2/M phases. However, the detailed nature of splenic disorders in these mice is not fully understood. Read More

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Gender differences in food allergy depend on the PPAR γ/NF-κB in the intestines of mice.

Life Sci 2021 May 8:119606. Epub 2021 May 8.

Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address:

Aims: Epidemiology shows that gender affects the incidence of food allergy. However, there is a lack of evidence of gender differences in food allergies and little is known about the mechanisms. The aim of this study was to excavate potential reasons for gender differences in food allergy based on estrogen. Read More

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Arbutin inhibits inflammation and apoptosis by enhancing autophagy via SIRT1.

Adv Clin Exp Med 2021 May 11. Epub 2021 May 11.

Department of Ophthalmology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, China.

Background: SIRT1 plays a protective role against diabetic retinopathy as it regulates inflammation, apoptosis and autophagy of cells.

Objectives: This study was designed to investigate the effects of arbutin and to identify a potential mechanism of action. Adult human retinal pigment epithelial (ARPE-19) cells were exposed to high glucose (HG) or treated with different concentrations of arbutin. Read More

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Genetic variants in are related to lower galectin-3 serum levels and clinical outcomes in systemic sclerosis patients: A case-control study.

Autoimmunity 2021 May 11:1-8. Epub 2021 May 11.

Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica - Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.

Introduction: Systemic sclerosis (SSc) is a rare complex disease characterized by vascular damage, autoimmunity, and extensive skin and internal organs fibrosis. Galectin-3 (Gal-3) is encoded by gene (Lectin, Galactoside-Binding, Soluble, 3; 14q22.3) and it has been reported to play a central role in self-tolerance, inflammation, and fibrosis. Read More

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Evaluation of CVC1302 for Improved Efficacy of FMD-Inactivated Vaccine in Oxidative Stressed Mice Generated with PCV2 Infection.

Viral Immunol 2021 May 10. Epub 2021 May 10.

National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

This study aimed to explore the effect of the immunopotentiator CVC1302 on foot-and-mouth disease (FMD) vaccination in animals placed under oxidative stress. We established oxidative stress models using porcine circovirus type 2 (PCV2)-infected PK-15 cells and mice model both and , respectively. The efficacy of CVC1302 on PK-15 cells or in addition to the FMD vaccine was evaluated by quantitative real-time polymerase chain reaction, histopathological and enzyme-linked immunosorbent assay (ELISA) analysis. Read More

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Protective effect of miR-138-5p inhibition modified human mesenchymal stem cell on ovalbumin-induced allergic rhinitis and asthma syndrome.

J Cell Mol Med 2021 May 11. Epub 2021 May 11.

Department of International Clinic, Qingdao Municipal Hospital, Qingdao, China.

The objective of the study is to evaluate the protective effects of human mesenchymal stem cells (hMSCs) modified with miR-138-5p inhibitor against the allergic rhinitis and asthma syndrome (ARAS). MiR-138-5p or negative control was transfected into hMSCs, and fluorescence-activated cell sorting was used to evaluate hMSC surface markers. Quantitative real-time PCR (qRT-PCR) was used to evaluate miR-138-5p, SIRT1, caspase-3, IL-6, IL-1β and TNF-α levels after TNF-α and IL-6 stimulations. Read More

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Serological responses to koi herpesvirus (KHV) in a non-cyprinid reservoir host.

J Fish Dis 2021 May 11. Epub 2021 May 11.

Aquatic Vaccine Unit, School of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, UK.

Koi herpesvirus (KHV) is a highly contagious virus that causes KHV disease (KHVD) inducing high mortality in carp and koi (Cyprinus carpio L.). In the late stage, latency occurs with very low, often non-detectable virus concentrations, which represents a challenge for virus detection. Read More

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Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system.

J Zhejiang Univ Sci B 2021 May;22(5):421-430

College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. Read More

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Evaluation of laboratory assays for anti-Platelet Factor 4 antibodies after ChAdOx1 nCOV-19 vaccination.

J Thromb Haemost 2021 May 10. Epub 2021 May 10.

Oxford University Hospitals NHS Foundation Trust.

Vaccine-induced immune thrombocytopenia and thrombosis (VITT) following ChAdOx1 nCOV-19 vaccine has been described, associated with unusual site thrombosis, thrombocytopenia, raised D-dimer and high titre immunoglobulin-G (IgG) class anti-Platelet Factor 4 (PF4) antibodies. Enzyme linked immunosorbent assays (ELISA) have been shown to detect anti-PF4 in patients with VITT, but chemiluminesence assays do not reliably detect them. ELISA assays are not widely available in diagnostic laboratories, and, globally, very few laboratories perform platelet activation assays. Read More

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TRESK Regulates Gm11874 to Induce Apoptosis of Spinal Cord Neurons via ATP5i Mediated Oxidative Stress and DNA Damage.

Neurochem Res 2021 May 10. Epub 2021 May 10.

Department of Anesthesiology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000, Guangdong Province, China.

Reportedly, TWIK-related spinal cord K (TRESK) deficiency in spinal cord neurons positively correlates with the mechanism underlying neuropathic pain (NP). However, the precise effects of TRESK on neurons of the spinal cord remain elusive. In the present study, we investigated the impact of TRESK silencing on spinal cord neurons to further elucidate the downstream mechanisms of TRESK. Read More

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Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation.

Med Sci Monit Basic Res 2021 May 11;27:e930887. Epub 2021 May 11.

National-Local Joint Engineering Research Center of Entomoceutics, Dali, Yunnan, China (mainland).

BACKGROUND The aim of this study was to determine the effect of kangfuxin liquid (KFXL) on inflammatory response, and its underlying mechanism in treating acute ulcerative colitis (UC) in mice induced by dextran sulfate sodium (DSS). MATERIAL AND METHODS Mice were provided drinking water containing DSS (3%) for 7 days to induce acute enteritis. The mice were divided into 6 groups: a control group, a DSS-induced (vehicle) group, a sulfasalazine (SASP) group, and low-, medium-, and high-dose kangfuxin liquid groups. Read More

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Early arteriosclerosis and its risk factors in subjects with prediabetes and new-onset diabetes.

Endocr Connect 2021 May 1. Epub 2021 May 1.

Q Lu, Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, China.

Background: We aimed to investigate early arteriosclerosis and its risk factors in populations with prediabetes and new-onset diabetes.

Materials And Methods: A total of 148 participants who did not have diabetes mellitus were assigned to three groups through an oral glucose tolerance test: the normal glucose tolerance group; the impaired glucose regulation, also known as prediabetes, group; and the new-onset type 2 diabetes mellitus group. The insulin resistance index was assessed using the Homeostatic Model Assessment of Insulin Resistance. Read More

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Sex differences in the association between serum BDNF and cognitive impairment in schizophrenia patients using various antipsychotics.

J Psychiatr Res 2021 Apr 30;138:492-499. Epub 2021 Apr 30.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Background: Cognitive impairment is one of the core symptoms of schizophrenia patients. There are often various differences in the efficacy of different antipsychotics in the treatment of cognitive impairment by sex. The purpose of this study was to explore whether there are gender differences in the association between serum BDNF levels and cognitive performance in patients with schizophrenia taking different antipsychotics. Read More

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Improving laboratory diagnostics in myasthenia gravis.

Expert Rev Mol Diagn 2021 May 10. Epub 2021 May 10.

Autoimmunity Laboratory, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, 16132 Genova (GE), Italy.

: Myasthenia gravis (MG) is a prototypical autoimmune disease, characterized by pathogenic autoantibodies targeting structures of the neuromuscular junction. Radioimmunoprecipitation assays (RIPAs) represent the gold standard for their detection. However, new methods are emerging to complement, or overcome RIPAs, also with the perspective of eliminating the use of radioactive reagents. Read More

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PU.1 Interaction with p50 Promotes Microglial-mediated Inflammation in Secondary Spinal Cord Injury in SCI Rats.

Int J Neurosci 2021 May 10:1-15. Epub 2021 May 10.

Department of Orthopedics, Changzhou Seventh People's Hospital, Changzhou, Jiangsu Province 213000, China.

Secondary spinal cord injury is the inflammatory damage to surrounding tissues caused by activated microglial-mediated neuroinflammatory responses. The nuclear factor-κB (p65/p50) pathway and PU.1 are closely correlated with inflammatory responses; thus, we examined the relationship and function between PU. Read More

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Macrophage inflammatory protein-1α in amniotic and cervical fluids in spontaneous preterm labor with intact membranes with respect to intra-amniotic inflammation.

J Matern Fetal Neonatal Med 2021 May 9:1-9. Epub 2021 May 9.

Institute of Clinical Immunology and Allergy, University Hospital Hradec Kralove, Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czech Republic.

Objective: Macrophage inflammatory protein 1α is a chemokine produced by various immune, epithelial, mesothelial, and fibroblast cells after exposure to bacterial lipopolysaccharide or pro-inflammatory molecules. The primary aim of this study was to determine MIP-1α concentrations in amniotic and cervical fluids from pregnancy with spontaneous preterm labor with intact membranes (PTL) with respect to the presence of intra-amniotic infection (both microbial invasion of the amniotic cavity and intra-amniotic inflammation) and sterile intra-amniotic inflammation (intra-amniotic inflammation alone). The secondary aim was to assess the diagnostic indices of MIP-1α in predicting intra-amniotic infection. Read More

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Dipeptidyl peptidase IV is required for endometrial carcinoma cell proliferation and tumorigenesis via the IL-6/STAT3 pathway.

J Obstet Gynaecol Res 2021 May 9. Epub 2021 May 9.

Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Aim: To study the functions and signaling pathways controlled by dipeptidyl peptidase IV (DPPIV) in endometrial carcinoma (EC).

Methods: DPPIV expression in EC cells was detected by flow cytometry, reverse transcription-polymerase chain reaction analysis and Western blot. Interleukin-6 (IL-6) expression in the supernatant was measured by enzyme-linked immunosorbent assay. Read More

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3D Printing of Monolithic Capillarity-Driven Microfluidic Devices for Diagnostics.

Adv Mater 2021 May 10:e2008712. Epub 2021 May 10.

Centre for Membrane Separations, Adsorption, Catalysis and Spectroscopy (cMACS), KU Leuven, Celestijnenlaan 200 F, Leuven, Box 2454, Belgium.

Rapid diagnostic testing at the site of the patient is essential when a fully equipped laboratory is not accessible. To maximize the impact of this approach, low-cost, disposable tests that require minimal user-interference and external equipment are desired. Fluid transport by capillary wicking removes the need for bulky ancillary equipment to actuate and control fluid flow. Read More

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Serum and cerebrospinal fluid phosphorylated neurofilament heavy protein concentrations in equine neurodegenerative diseases.

Equine Vet J 2021 May 9. Epub 2021 May 9.

Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA.

Background: Currently, there is little information regarding the concentrations of phosphorylated neurofilament heavy protein (pNfH) in the serum and cerebrospinal fluid (CSF) of horses with neurodegenerative diseases. Specifically, pNfH concentrations have not yet been evaluated in horses with equine neuroaxonal dystrophy/equine degenerative myeloencephalopathy (eNAD/EDM).

Objectives: To determine pNfH concentrations using a commercial enzyme-linked immunosorbent assay (ELISA) in serum and CSF from control horses and horses with eNAD/EDM, cervical vertebral compressive myelopathy (CVCM) and Shivers. Read More

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Development of an RT-PCR assay to detect genetically divergent wheat streak mosaic virus isolates for plant quarantine inspections in South Korea.

Virusdisease 2021 Mar 17;32(1):150-154. Epub 2021 Mar 17.

Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon, 39660 Republic of Korea.

Wheat streak mosaic virus (WSMV), a member of the genus in the family , has been designated as a plant quarantine pathogen in South Korea. Several diagnostic methods can be applied to diagnose viral infections in plants, but polymerase chain reaction and enzyme-linked immunosorbent assay, which can identify viral species with speed and accuracy, are mainly used in Korean plant quarantine. Many variants of different viral species with highly divergent genomic sequences are constantly being discovered by high-throughput sequencing technology. Read More

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Para-Clinical and Immunological Evaluation in Buerger's Disease as a Suspected Autoimmune Disease: Case Series.

Rep Biochem Mol Biol 2021 Jan;9(4):379-384

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: Autoimmunity causes the loss of normal immune homeostasis and involves the presence of autoantibodies and inflammation. Thromboangiitis obliterans or Buerger's disease (BD) refers to a type of vascular obstructive syndrome, with tobacco exposure accounting for disease formation and progression. However, the current understanding of autoimmunity is unclear in the context of BD, and the scientific findings are not enough to support autoimmune mechanisms. Read More

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January 2021

Mechanism of and Ameliorates Hypertensive Renal Damage.

Biomed Res Int 2021 21;2021:5598351. Epub 2021 Apr 21.

Department of Nephrology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.

Hypertensive-induced renal damage (HRD) is an important public health and socioeconomic problem worldwide. The herb pair - (RA-) (RS) is a common prescribed herbal formula for the treatment of HRD. However, the underlying mechanisms are unclear. Read More

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The significance of coagulation and fibrinolysis-related parameters in predicting postoperative venous thrombosis in patients with breast cancer.

Gland Surg 2021 Apr;10(4):1439-1446

Department of Laboratory, The Second Hospital of Jilin University, Changchun, China.

Background: To explore the expression level of coagulation and fibrinolysis-related indexes in the plasma of breast cancer patients after surgery, and explore their predictive value for deep venous thrombosis (DVT).

Methods: From May 2016 to May 2019, 63 patients with lower extremity DVT after radical mastectomy in our hospital were selected as the thrombus group, and 69 patients without venous thrombosis after radical mastectomy were selected as the control group. The levels of D-dimer (D-D) and fibrinolytic product (FDP) were measured by latex enhanced immunoturbidimetry, Fibrinogen (FIB) levels were measured using the von Clauss method, thrombin antithrombin complex (TAT) and thrombomodulin (TM) levels were measured by enzyme-linked immunosorbent assay (ELISA), and the evaluation value of coagulation markers on tumor thrombosis was analyzed by receiver operating characteristic curve (ROC) curve analysis. Read More

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Increased Circulatory Interleukin-17A Levels in Patients with Progressive and Leukotrichial Vitiligo.

Dermatol Res Pract 2021 21;2021:5524566. Epub 2021 Apr 21.

Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.

Background: Vitiligo is a chronic condition characterized by skin depigmentation. Although not life-threatening, it significantly impacts quality of life. The pathophysiology of vitiligo remains poorly understood, and treatment options are limited. Read More

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A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1.

Front Immunol 2021 23;12:670626. Epub 2021 Apr 23.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Fibrinogen-like protein 1 (FGL1), a member of the fibrinogen family, is a specific hepatocyte mitogen. Recently, it has been reported that FGL1 is the main inhibitory ligand of lymphocyte activating gene 3 (LAG3). Furthermore, the FGL1-LAG3 pathway has a synergistic effect with programmed death 1 (PD-1)/programmed death ligand 1 (PD-L1) pathway and is regarded as a promising immunotherapeutic target. Read More

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Discovering Panel of Autoantibodies for Early Detection of Lung Cancer Based on Focused Protein Array.

Front Immunol 2021 23;12:658922. Epub 2021 Apr 23.

Department of Oncology, Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.

Substantial studies indicate that autoantibodies to tumor-associated antigens (TAAbs) arise in early stage of lung cancer (LC). However, since single TAAbs as non-invasive biomarkers reveal low diagnostic performances, a panel approach is needed to provide more clues for early detection of LC. In the present research, potential TAAbs were screened in 150 serum samples by focused protein array based on 154 proteins encoded by cancer driver genes. Read More

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Huanglong Antitussive Granule Relieves Acute Asthma Through Regulating Pulmonary Lipid Homeostasis.

Front Pharmacol 2021 23;12:656756. Epub 2021 Apr 23.

Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Asthma is a respiratory disease with chronic airway inflammatory, and individuals with asthma exacerbations is one of the most frequent causes of hospitalization. Huanglong antitussive granule (HL Granule), a Chinese proprietary herbal medicine, has been proved to be effective in the clinical treatment of pulmonary disease. This study is devoted to the pharmacodynamics of HL Granule in acute asthma and the possible mechanism from the perspective of lipidomics. Read More

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β-Caryophyllene Ameliorates MSU-Induced Gouty Arthritis and Inflammation Through Inhibiting NLRP3 and NF-κB Signal Pathway: In Silico and In Vivo.

Front Pharmacol 2021 23;12:651305. Epub 2021 Apr 23.

Xiangya School of Public Health, Central South University, Changsha, China.

Gouty arthritis serves as an acute reaction initiated by the deposition of monosodium urate (MSU) crystals around the joints. In this study, the anti-inflammatory effects of phytochemical β-caryophyllene on MSU crystal-induced acute gouty arthritis and in silico were explored. Through bioinformatics methods and molecular docking, it screened the specific influence pathway of β-caryophyllene on gout. Read More

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Protective effect and mechanisms of action of Mongolian medicine Sulongga-4 on pyloric ligation-induced gastroduodenal ulcer in rats.

World J Gastroenterol 2021 Apr;27(16):1770-1784

School of Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia Autonomous Region, China.

Background: Sulongga-4 (SL-4) is a herbal formula used in traditional Mongolian medical clinics for the treatment of peptic ulcers and gastroenteritis, even though its pharmacological mechanism has not been well characterized.

Aim: To evaluate the protective effect and identify the mechanisms of action of SL-4 on gastroduodenal ulcer induced by pyloric ligation (PL) in rats.

Methods: PL was performed to induce gastric and duodenal ulcers in rats, which were then treated with oral SL-4 (1. Read More

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